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Yorodumi- EMDB-26615: Complex of UBE2O with NAP1L1 and ubiquitylated uL2 (focused on UB... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-26615 | ||||||||||||||||||
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Title | Complex of UBE2O with NAP1L1 and ubiquitylated uL2 (focused on UBE2O-Ub) | ||||||||||||||||||
Map data | Sharpened map | ||||||||||||||||||
Sample |
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Biological species | Homo sapiens (human) | ||||||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.5 Å | ||||||||||||||||||
Authors | Yip MCJ / Sedor SF / Shao S | ||||||||||||||||||
Funding support | United States, 5 items
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Citation | Journal: Nat Struct Mol Biol / Year: 2022 Title: Mechanism of client selection by the protein quality-control factor UBE2O. Authors: Matthew C J Yip / Samantha F Sedor / Sichen Shao / Abstract: The E2/E3 enzyme UBE2O ubiquitylates diverse clients to mediate important processes, including targeting unassembled 'orphan' proteins for quality control and clearing ribosomes during erythropoiesis. ...The E2/E3 enzyme UBE2O ubiquitylates diverse clients to mediate important processes, including targeting unassembled 'orphan' proteins for quality control and clearing ribosomes during erythropoiesis. How quality-control factors, such as UBE2O, select clients on the basis of heterogeneous features is largely unknown. Here, we show that UBE2O client selection is regulated by ubiquitin binding and a cofactor, NAP1L1. Attaching a single ubiquitin onto a client enhances UBE2O binding and multi-mono-ubiquitylation. UBE2O also repurposes the histone chaperone NAP1L1 as an adapter to recruit a subset of clients. Cryo-EM structures of human UBE2O in complex with NAP1L1 reveal a malleable client recruitment interface that is autoinhibited by the intrinsically reactive UBC domain. Adding a ubiquitylated client identifies a distinct ubiquitin-binding SH3-like domain required for client selection. Our findings reveal how multivalency and a feed-forward mechanism drive the selection of protein quality-control clients. | ||||||||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_26615.map.gz | 13.3 MB | EMDB map data format | |
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Header (meta data) | emd-26615-v30.xml emd-26615.xml | 17.7 KB 17.7 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_26615_fsc.xml | 12.1 KB | Display | FSC data file |
Images | emd_26615.png | 77.6 KB | ||
Others | emd_26615_additional_1.map.gz emd_26615_additional_2.map.gz emd_26615_half_map_1.map.gz emd_26615_half_map_2.map.gz | 133 MB 117.1 MB 118.3 MB 118.3 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-26615 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-26615 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_26615.map.gz / Format: CCP4 / Size: 149.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | Sharpened map | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.825 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: deepEMhancer sharpened map
File | emd_26615_additional_1.map | ||||||||||||
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Annotation | deepEMhancer sharpened map | ||||||||||||
Projections & Slices |
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Density Histograms |
-Additional map: Unsharpened map
File | emd_26615_additional_2.map | ||||||||||||
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Annotation | Unsharpened map | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map 1
File | emd_26615_half_map_1.map | ||||||||||||
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Annotation | Half map 1 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map 2
File | emd_26615_half_map_2.map | ||||||||||||
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Annotation | Half map 2 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Complex of UBE2O with NAP1L1 and ubiquitylated uL2 (focused on UB...
Entire | Name: Complex of UBE2O with NAP1L1 and ubiquitylated uL2 (focused on UBE2O-Ub) |
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Components |
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-Supramolecule #1: Complex of UBE2O with NAP1L1 and ubiquitylated uL2 (focused on UB...
Supramolecule | Name: Complex of UBE2O with NAP1L1 and ubiquitylated uL2 (focused on UBE2O-Ub) type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: #1-#4 |
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Source (natural) | Organism: Homo sapiens (human) |
Recombinant expression | Organism: Homo sapiens (human) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 1 mg/mL |
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Buffer | pH: 7.5 |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.4000000000000001 µm |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 59.6 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |