EMDB-25381: Composite map of ciliary C1 central pair apparatus isolated from ...

Basic information

Entry

Database: EMDB / ID: EMD-25381

• Title: Composite map of ciliary C1 central pair apparatus isolated from Chlamydomonas reinhardtii
• Map data: Ciliary C1 central pair apparatus isolated from Chlamydomonas reinhardtii

Sample

• Complex: C1 central pair apparatus complex
  • Protein or peptide: x 41 types
• Ligand: x 5 types

Function / homology

Function:

axonemal central apparatus / axonemal central pair projection / axonemal central apparatus assembly / cilium movement involved in cell motility / cilium movement / axoneme assembly / guanylate kinase activity / calcium-dependent cysteine-type endopeptidase activity / phosphopyruvate hydratase / phosphopyruvate hydratase complex / phosphopyruvate hydratase activity / adenylate kinase / adenylate kinase activity / misfolded protein binding / motile cilium / myosin phosphatase activity / protein serine/threonine phosphatase activity / protein-serine/threonine phosphatase / mitochondrial respiratory chain complex I / axoneme / chaperone cofactor-dependent protein refolding / cilium assembly / cellular response to unfolded protein / enzyme regulator activity / protein folding chaperone / heat shock protein binding / tubulin binding / electron transport chain / ATP-dependent protein folding chaperone / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / glycolytic process / cilium / structural constituent of cytoskeleton / microtubule cytoskeleton organization / microtubule cytoskeleton / unfolded protein binding / mitotic cell cycle / protein refolding / microtubule binding / microtubule / transcription coactivator activity / cytoskeleton / calmodulin binding / hydrolase activity / negative regulation of cell population proliferation / phosphorylation / GTPase activity / calcium ion binding / regulation of DNA-templated transcription / GTP binding / magnesium ion binding / ATP hydrolysis activity / mitochondrion / proteolysis / ATP binding / membrane / metal ion binding / nucleus / cytosol / cytoplasm

Domain/homology:

Sperm-associated antigen 17 / Coiled-coil domain-containing protein 180 / Cilia- and flagella-associated protein 54 / Domain of unknown function DUF4456 / Domain of unknown function DUF4455 / Primary ciliary dyskinesia protein 1 / Flagellar C1a complex subunit C1a-32 / Deleted in lung and esophageal cancer protein 1 / Cilia- and flagella-associated protein 99 / Cilia- and flagella-associated protein 46 / Leucine-rich repeat-containing protein 72 / Domain of unknown function (DUF4455) / Domain of unknown function (DUF4456) / Flagellar C1a complex subunit C1a-32 / Cilia- and flagella-associated protein 54 / Associate of Myc 1 / Hydrocephalus-inducing-like / CH-like domain in sperm protein / CH-like domain in sperm protein / Adenylate kinase, active site lid domain superfamily / Peptidase C2, calpain, catalytic domain / Calpain family cysteine protease / Cysteine proteinase, calpain-type, catalytic domain profile. / Calpain-like thiol protease family. / Possible plasma membrane-binding motif in junctophilins, PIP-5-kinases and protein kinases. / MORN motif / MORN repeat / Serine-threonine protein phosphatase, N-terminal / Serine-threonine protein phosphatase N-terminal domain / Transglutaminase/protease-like homologues / Transglutaminase-like / Adenylate kinase, conserved site / Adenylate kinase signature. / Adenylate kinase/UMP-CMP kinase / Enolase / Enolase, conserved site / Enolase, C-terminal TIM barrel domain / Enolase, N-terminal / Enolase, C-terminal TIM barrel domain / Enolase, N-terminal domain / Enolase signature. / Enolase, C-terminal TIM barrel domain / Enolase, N-terminal domain / Serine/threonine specific protein phosphatases signature. / Protein phosphatase 2A homologues, catalytic domain. / Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase / IQ calmodulin-binding motif / Guanylate kinase-like domain profile. / Guanylate kinase-like domain / Heat shock hsp70 proteins family signature 2. / Heat shock hsp70 proteins family signature 1. / Heat shock hsp70 proteins family signature 3. / Heat shock protein 70, conserved site / Guanylate kinase/L-type calcium channel beta subunit / Guanylate kinase / Guanylate kinase homologues. / Heat shock protein 70kD, peptide-binding domain superfamily / Heat shock protein 70 family / Hsp70 protein / Calponin homology domain / Heat shock protein 70kD, C-terminal domain superfamily / Armadillo/beta-catenin-like repeat / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / Calponin homology (CH) domain / Short calmodulin-binding motif containing conserved Ile and Gln residues. / Enolase-like, N-terminal / Calponin homology domain / Enolase-like, C-terminal domain superfamily / CH domain superfamily / Calponin homology (CH) domain profile. / Calcineurin-like phosphoesterase domain, ApaH type / Calcineurin-like phosphoesterase / IQ motif profile. / Armadillo/beta-catenin-like repeats / Armadillo / Metallo-dependent phosphatase-like / IQ motif, EF-hand binding site / Leucine rich repeat / Leucine-rich repeat, typical subtype / Leucine-rich repeats, typical (most populated) subfamily / Tubulin-beta mRNA autoregulation signal. / Alpha tubulin / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Leucine-rich repeat profile. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / Leucine-rich repeat / EF-hand domain pair

Component:

Calponin-homology (CH) domain-containing protein / Uncharacterized protein / EF-hand domain-containing protein / adenylate kinase / Flagellar associated protein / Spermatogenesis-associated protein 17 / DUF4455 domain-containing protein / Sperm-associated antigen 6 / LRRcap domain-containing protein / Deleted in lung and esophageal cancer protein 1 / EH domain-containing protein / MYND-type domain-containing protein / ASH domain-containing protein / V-SNARE coiled-coil homology domain-containing protein / Flagellar associated protein 174 / Cilia- and flagella-associated protein 46 / Calmodulin / Predicted protein / Cilia- and flagella-associated protein 99 / Centrosomal protein of 162 kDa / Cilia- and flagella-associated protein 54 / Flagellar associated protein / Heat shock protein 70 / Flagellar associated protein / phosphopyruvate hydratase / FAP221 / Cilia- and flagella-associated protein 74 / Tubulin beta-1/beta-2 chain / Tubulin alpha-1 chain / Central apparatus associated protein C1a-86 / Central apparatus associated protein C1a-34 / Central apparatus associated protein C1a-32 / Central apparatus associated protein C1a-18 / adenylate kinase / PF6 protein / Serine/threonine-protein phosphatase

• Biological species: Chlamydomonas reinhardtii (plant) / Chlamydomonas smithii (plant)
• Method: single particle reconstruction / cryo EM / Resolution: 3.8 Å
• Authors: Gui M / Wang X / Dutcher SK / Brown A / Zhang R

Funding support

United States, 1 items

Organization	Grant number	Country
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)		United States

• Citation: Journal: Nat Struct Mol Biol / Year: 2022; Title: Ciliary central apparatus structure reveals mechanisms of microtubule patterning.; Authors: Miao Gui / Xiangli Wang / Susan K Dutcher / Alan Brown / Rui Zhang / ; Abstract: A pair of extensively modified microtubules form the central apparatus (CA) of the axoneme of most motile cilia, where they regulate ciliary motility. The external surfaces of both CA microtubules are patterned asymmetrically with large protein complexes that repeat every 16 or 32 nm. The composition of these projections and the mechanisms that establish asymmetry and longitudinal periodicity are unknown. Here, by determining cryo-EM structures of the CA microtubules, we identify 48 different CA-associated proteins, which in turn reveal mechanisms for asymmetric and periodic protein binding to microtubules. We identify arc-MIPs, a novel class of microtubule inner protein, that bind laterally across protofilaments and remodel tubulin structure and lattice contacts. The binding mechanisms utilized by CA proteins may be generalizable to other microtubule-associated proteins. These structures establish a foundation to elucidate the contributions of individual CA proteins to ciliary motility and ciliopathies.

History

Deposition	Nov 5, 2021	-
Header (metadata) release	Apr 13, 2022	-
Map release	Apr 13, 2022	-
Update	Jun 8, 2022	-
Current status	Jun 8, 2022	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_25381.map.gz / Format: CCP4 / Size: 1000 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Ciliary C1 central pair apparatus isolated from Chlamydomonas reinhardtii
• Voxel size: X=Y=Z: 1.39 Å

Density

Contour Level	By AUTHOR: 0.2
Minimum - Maximum	0.0 - 2.0726204
Average (Standard dev.)	0.0069481703 (±0.053195074)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 640 640 640 Spacing 640 640 640
Cell	A=B=C: 889.6 Å α=β=γ: 90.0 °

Supplemental data

Additional map: wedge mask, core

• File: emd_25381_additional_1.map
• Annotation: wedge mask, core

Additional map: wedge mask, outer shell

• File: emd_25381_additional_10.map
• Annotation: wedge mask, outer shell

Additional map: wedge mask, outer shell

• File: emd_25381_additional_11.map
• Annotation: wedge mask, outer shell

Additional map: wedge mask, outer shell

• File: emd_25381_additional_12.map
• Annotation: wedge mask, outer shell

Additional map: wedge mask, outer shell

• File: emd_25381_additional_13.map
• Annotation: wedge mask, outer shell

Additional map: wedge mask, outer shell

• File: emd_25381_additional_14.map
• Annotation: wedge mask, outer shell

Additional map: consensus refinement

• File: emd_25381_additional_15.map
• Annotation: consensus refinement

Additional map: mask for fsc

• File: emd_25381_additional_16.map
• Annotation: mask for fsc

Additional map: mask for consensus refinement

• File: emd_25381_additional_17.map
• Annotation: mask for consensus refinement

Additional map: wedge mask, core

• File: emd_25381_additional_2.map
• Annotation: wedge mask, core

Additional map: wedge mask, core

• File: emd_25381_additional_3.map
• Annotation: wedge mask, core

Additional map: wedge mask, core

• File: emd_25381_additional_4.map
• Annotation: wedge mask, core

Additional map: wedge mask, core

• File: emd_25381_additional_5.map
• Annotation: wedge mask, core

Additional map: wedge mask, core

• File: emd_25381_additional_6.map
• Annotation: wedge mask, core

Additional map: wedge mask, core

• File: emd_25381_additional_7.map
• Annotation: wedge mask, core

Additional map: wedge mask, outer shell

• File: emd_25381_additional_8.map
• Annotation: wedge mask, outer shell

Additional map: wedge mask, outer shell

• File: emd_25381_additional_9.map
• Annotation: wedge mask, outer shell

Sample components

Entire : C1 central pair apparatus complex

• Entire: Name: C1 central pair apparatus complex

Components

• Complex: C1 central pair apparatus complex
  • Protein or peptide: Flagellar associated protein
  • Protein or peptide: FAP42
  • Protein or peptide: FAP7
  • Protein or peptide: FAP81
  • Protein or peptide: FAP216
  • Protein or peptide: FAP297
  • Protein or peptide: FAP114
  • Protein or peptide: FAP305
  • Protein or peptide: FAP119
  • Protein or peptide: Calmodulin
  • Protein or peptide: FAP101
  • Protein or peptide: FAP15
  • Protein or peptide: FAP227
  • Protein or peptide: FAP105
  • Protein or peptide: PF6
  • Protein or peptide: FAP219
  • Protein or peptide: FAP99
  • Protein or peptide: CPC1
  • Protein or peptide: PF16
  • Protein or peptide: FAP108
  • Protein or peptide: Hydin
  • Protein or peptide: FAP221
  • Protein or peptide: FAP194
  • Protein or peptide: Tubulin alpha
  • Protein or peptide: Tubulin beta
  • Protein or peptide: FAP74
  • Protein or peptide: FAP69
  • Protein or peptide: FAP54
  • Protein or peptide: FAP46
  • Protein or peptide: FAP275
  • Protein or peptide: Unknown protein
  • Protein or peptide: FAP47
  • Protein or peptide: FAP279
  • Protein or peptide: FAP266
  • Protein or peptide: FAP76
  • Protein or peptide: Unknown protein
  • Protein or peptide: Unknown protein
  • Protein or peptide: Unknown protein
  • Protein or peptide: FAP246
  • Protein or peptide: Phosphopyruvate hydrataseEnolase
  • Protein or peptide: Heat shock protein 70AHeat shock response
• Ligand: ADENOSINE-5'-DIPHOSPHATE
• Ligand: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
• Ligand: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate
• Ligand: MAGNESIUM ION
• Ligand: GUANOSINE-5'-DIPHOSPHATE

Supramolecule #1: C1 central pair apparatus complex

• Supramolecule: Name: C1 central pair apparatus complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#41
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)

Macromolecule #1: Flagellar associated protein

• Macromolecule: Name: Flagellar associated protein / type: protein_or_peptide / ID: 1 / Number of copies: 12 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 10.336616 KDa

Sequence

String:

MSESQKETFR KYLEQAGAID VLVKVLVQLY EEPSKPKTAL DYIKQCLGSP TPAEYEAVVA ERDGLQKQLE EANQLIAELQ SRVQSLEAA AETA

Macromolecule #2: FAP42

• Macromolecule: Name: FAP42 / type: protein_or_peptide / ID: 2 / Number of copies: 4 / Enantiomer: LEVO / EC number: adenylate kinase
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 269.1725 KDa

Sequence

String:

MAPKAPAPGA KPGDPKAKGA APVERVSFLD KAFPTWVDLP PDKDPPSNEK YEDLAGLVLP PETQMFLDTW KRPEELVLNS PDVPMVTTV APPPPPEPTG GKEGKADKAA AKNAPVVLSR DLEGSLQAGH RTFEWLQAVF VMVISAQKAI KPGEYLWELI Y PKDKDGQA TKSPNGKYKI KLYIMDAWRT VVVDDRIPVD LFGRPLLVNA RPIQLWPLLL SKAVLKVLAA NRILHCGLPH QA AAFQLLT GWPQEDLLDS LSGTRLSGGR LFDRLEEAVR GNEERTERHA VAAVTLVKRA LPERPPPRLI VLVGPSGVGR GAL LQRLVG ELPDKFGLTV SHTTRPPREH EVQGGDYFFC EMGAFREEAA AGRLLENAPV PSANDGVHLY GTSFATVREV AATG KLCLM GLDVQGVRSL RANKRIDGLY VFVSPPSLDE LERRQRGRLK EAETTIAKRL AWARAELDKA AGGKSVHSGV PAGGA GSAS GGAGAVIDYV IDNGDDGDKV YLDVKEAIST LSPIIRNRLH GLPAYVLDYA DLIAPNLVEK PFLKPVVITG PTTGER RAL MEQLVREFPD VFAYPRHTTT RPAHEDALYR VDAADSPPEQ VEFEVIKTKD GEEVKMSRPT FTSVSPTEFT SAARSGA LL EHHTELFKHP LVTRQWGVTA DAIKEVIRAG RLPLMECETE GAEMLKKRGI DCLTLFLKPP SMDVFETRLR DHLTETDE E IAARLDMARR EMEAAAAAGS PFDATIVNDD PEAAYAELTR LISRCRPDII VPEEDRQAEP PPPPKQPVLV LCGPSAAGR SALAKQLLST FPDKFTAPGI TTDRKPTKGE ISTAACTFVG PKDLVKMQAE GLIAYMRAPE EKGAGTTAIT NAALLRVAGE NKVAVLELP DGGAVVPELR KGAVLKDALY VFVASPGQLR ESLEREAEAA AAEAAMKRSK STKGGAAAAA AAAAGGAGAD V DAALAELD TQAGGAASSG ELYDTVVRED DIMDLISAVR MALAAHVPNV VPPPYRPLVV AGAFGTGKRK LLARLFDALP GR FAVPVIT TTRQPGPTDP DQAREGMAII SKAEADAVTA AGGWATRREV LGEVYGITVA AVKKVGASGR VPIIEVDHVE DAA ALRARG FDAAYLFIGM EDMGKLFHVI NEELSANPPL GYELQDAVNQ FFAAAKAEMA ASRQPGLFDE WVHHVHDAPD PSFI RLAEA VHRSYPDVVT RHFVWGYGRQ LWDEAVRVHG HRPLKVMVLG PAASGKSTQC DMLAAHFGMP HVNVGDLLFE EVRKK TPLG LEAKEYMDAS KTVPDRFFFE VLTQRLAEPD CVARGWLLDG FPHTAEQCEE LGRRGISPDK VLLLEGEHAV LLDRSR YRR YDPATGKVYH MPGDNDDALS PPIQPERPDG NLDAEVVARL VPRHDDSDEN VSARLALSDA HVAALRDAYE DICLRLN ST SDPRVMFQQA LDYLTLEARV PELAVVPSTS LKELQYVVAT TLRHRRQQLL QLQQDDGRTY WVDSKEVLGA NAHCALLC Q DPALFPTSHQ LRRVDTGKLS RVALLYVDSP EPVKLLSSLF TGPQYGLLED PPTPRNVLVL AGPAGVGKTA VLRMLLQQL GEQLELVPVV TSRPPLRREL GTRVVLHPAA ADGTGGEVAE GQPGLGPADR DTIATACCVS LDAMEDAEAA GQLLGTCEGW DGHRYGVSR AALQAAWAAG KLPVVEGPLE LALALKELNS VMVPLQSAHP PALSTQPSGH PALSTQASGH PGALSTQASG H PGALSAQP STVHDLHGHG TESGEAGHGT EMGPLLSARV VYLAVDVAEQ DVRLRLQDQR EEAAVGVCIA AAVREAETIR AM QKALDDH AAAVKAAANK PPSRAVTPPQ GGRTAGAAAA AAAAAAAAAA AAAPPPPPPS LDVVMQATDA VVAFHAAKRL AAD SWRRPR AQVAGQLVLE AFDWRAPGGR PGRTVQRLRT LMANAGLLEL PRGRHVLRIN SDPLFLHAVT FMSSTPCTVG EYSQ VMPLC APEAHVVPLE GRYGEAPAGA VGVLFRYCFA LHQAATLSAY LSINGEDMRA ATRLLLMDRA SGVARPVPSN RLHAT ELPA SGAGYCLLAL YDTGSRGVNE EGTYCLTVTC TSPLAGAAAG AGALTEVPCH RLDSFVEAYR PNSRATLSRH VITCTT TTQ LALVAAAEPR LPFRLTLQEA PTGREITWLT SPDYPTLVSA ASVRGVTPAS VVPAPVGAEG AAAGAGASPP PVPDGFA LV ADVTLKPGKY LVSCTLDAAD CPAGMQPDPR TGALPEGAEP VKLRLWVAPS ADEKSCTVLA DNALAKYVQG VYDKWNTA P VQLIAQPPPG GAATKGKGGA GAPTAGGGAK AAVGARPAVA ANMLEKYKAE VANGGAAVAP AAVGGDGSPL PGAVTRVIK DGSTLVLDPT AQLRVLPAGG KPGSGPVLLS AEQLAERQAA ATQAAASEGA ARLASVGAAL QASKGERAGF KQRQANNFAE WRASLMAAQ REAASKRHEL ASQVKAAAVP PPTPPGPEPS APSASHGKGG AHGGAGRPAL A

Macromolecule #3: FAP7

• Macromolecule: Name: FAP7 / type: protein_or_peptide / ID: 3 / Number of copies: 20 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 54.721914 KDa

Sequence

String:

MPSPKRSGLG SGRLIGGQAS TTSLGSPGAG TSQFQINHEN TLRKNRNHFQ GQIEQYSIDY HSSHNKALAE LPVLQEQHAL EIEEYQNAI EETVTRHLGR IAQLRQDYSL LIKEASRRHM ELQQRRFAGA RVDIPQVAQQ PIAGLPALSA QPQIVAPSAV P NGSLAASR SGNVSTSSEQ AGSHAMGQVN PNAYLRTLNN DAAAAMSQYN TRPLPATPGG IAALSISTPA SPLTARLATP SE ATARNSD FARLEAMHEK YMGRTKSQHE QGIAARINEV SNWQQTFAAC MAALAQHQSS ALAHLSSCVE EAEAWHAQQR GAL SAAHDE VMTEAERLSR QLEAAQTAAA AKLNGLLASF LERVLPSGEV GLAEAQASYT RSAANLRNEH VAALEAAEAA LRAL VPRHA AMRQHMSASY SSGLASHEAA LAAAGGHYDS RGIPELRRQY QDAESRHRDT LAAIRAEHLK GLAGSRDGWM GEAAA LLEE YRARMQELKQ QYMLAYDVNL TEV

Macromolecule #4: FAP81

• Macromolecule: Name: FAP81 / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 234.483781 KDa

Sequence

String:

MSSAHILSTF QSTFPGLYQA PKKGEDEPPP EAPAPEPVTQ HDDEPDQYST RIAGITSKFE RMRASADEME QYLRSAAEDA KEAEARALA KADEDFTPAW RNVGLPLKPS HLHLDHGAMA GARLVNPKAI VQEYQAIKGR EVLNPPRIAE YADTSAKPNY L KSTHAMEE RKMRTMSPDR TARIQALSAR HLAWQTLTPE EVAAKMEEAE QRRRQLGLKM PRAQFELEQK IIQSMHHKLT FL RNPRHPL PPAVKTLMEL RPDANRWVGP RTTVLEGVKP PIKADMTSRP DQVFVVEPAE VTFTNYAVGR AYEQVVRVRN VTA VSRSLR IFPPASQYFH ASLPRFPGEV GVLAPGMAAE VTLRFCPDSL GDYEDAIAVD ATHSRQTVPL RARRPPPSLT LPEE IDMGQ VVIGNVKTEQ VTFKNMGGAG RFRIVPEAHW PDFAMDAPTD RAVVGQFKIW PLYFEMAAGE QLGLNVSYEP TEWGN TEER LVLVCDNCQV KTFSLSGNAV GVDVLLHSVD GRMLEPRELD LPLWFGECAP GAGFSKTVSV RNTTKLPFAF EWGLTK FPQ VQNRRRANEP LQTEAQYDEE QDDEGHVLLV DNKSLRGTSP LRLGTGGGGA AAAPPAVGGG AGGGAGGGAG GVSSSAA GV NGSVAQAPGG GVAGAKPPGP MKALAGAENA GTPWGVHCGN EAHGPDPLAL GAVVEDLFRV VPRSGVLQPG EVMEFLVT F TPPGQARYER WAQLRVDRKP ISVPSGASPM VRGSGSGTGR SHAAIAASAT CDVLVAEVGL EGLGCPVQLS AAPRLVSLP GKLMPAEGTT RHVTLRNPTR AQVVVRATVD NPAIAVSPSE FRMPSLGAIS LAVTVRAPPD AAPGPLSGRV LLEVEHGPPV PIEVRAAVG SSYARLITPR INFGDVPLSG SSEQRLVIRN MSATCPTPWS IRELTPALVA AEKSRLLRAQ LLQSSRMLDP Q AAAAAIDA LVQEEEEAEA AAAAREEEEA VGYRGASVTG HHARFAEAQR SPAASTSGAL VALPASAAER RAVFAAGRHT DS SLAQALP PPDTTHVTFE PSSGVLEPNQ ELTVRVTCHA LTDGRHRSII QLRSGAPHAG GGPDGGLHME CLEAFACVVT PAC VVDRPV MDLGVTFVGV QVRQTLYLTN LSQLPVLYRW TAEAEDEGSQ TAGLAELKIK PDHGELEPGE DVEIQVRYTP RYPG PCVMY GVCELEGAPE PLGFRVSSAI HGLDVTYDLL TQEQYDDYMA HDQAAAALGS TGPKGAAAMA VAAAAAGGAN TGSGG YYDS ESGEVAGAGG EGGGPSRAEE IAAALDKVNF MRVGRHGKAE VDVEAFSGLT HLPPDVLSRV ASHRHASTSA AGSKAA SRR ASARPGAQAR PRSGAAAGGG VAAWPPPTPQ RHLVADFGHN VPLGETRQMY LVVTNRTAMH TSIRTWLERF GVADASR FV RGTESGAAPP GGGADKAGGA EGGAGKGGAS RRQTKDEDAH HPQGPKLSRY SKYTPIKLAG TDAEHRAPFR ADKGNEMM A TRRLQEEADE ALGNKGLAVS VTPPESTLEP WSRLVLTVSC FNDMCGAYMD MMHVKVGDLP ARDIPVLVGV SGTPLVVQR ERVLVRGLRA RSWRTDLEWG QVPQGVEQTR TFYVFNTGSL DMHLAWEARR YHDYVDLERL PPTTDVGAGG TGQHDSLWGG APGTLRDTR AGMKLFDVKL QPDERAGCVR LATERHADPT DDVPFRVEPE EQVIKGNTTA KFTVTFCASE SRRHGGYLHG T QRVFSPES PLELRVWTAG ENADRVGALL SGTFHPYAGA PPTPLQPLRV DLGAQAQACR LEPDGQTDLS WVVTSIQQPG SH AAFVRSV TLSNTAHCPQ VFSLDVEGPW DMVAASPSVP QDPVAYRGTS TLLGPAAASG RLGTSAADGG LTFLPPGESV DVT LRFSPG KGDMEALPVR FAAMQAKQRV IETVNDYKNT GALCITFANG DSQSLPLVAE MLHPRLEVKP RKLDFKKVHL QSPK EMFVM LSNPTNVDAA WAVTVEGHKP RFPTLPGAGA AASAAAAKEA KEEAAAAAAA AGGGGSASAQ PTPRSASGGN LAGEA SAPD SRAASAVSGA SRPATVDGGA AGAAAPAGGV PPPPKLPGAG GPGTLPGVTG IIAEARIGPY VVKPASGVLS GRGLGM PRS QRISITFAPT EAEAYEGELI FAVLRGKQCS VDVDGEGSIE ETDETKGNLF VI

Macromolecule #5: FAP216

• Macromolecule: Name: FAP216 / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 79.183719 KDa

Sequence

String:

MASIGSAPGA DPLRPSANAA AKKCLEDLYE KLEATVKKRL RIKLGGVQTV DWSKLSVALN SAQPKPKPPI LPSSRTWRTD LAVEMKPEI KQQLREVFIK YCGWGDKQNF MFISRSQFIR FARDMGMCNE EIDALGLSGL FDKILLGSLD PGAASRLAMS D FVLAVTGL GTKLCPSPTV QESFETVVTR YVLPRLGQAL PEPDPIDSAL LHLDVMGTLE RLRPKLVVLW ETYMQQQHHS GT GSGHDSP TAGNSSGGGE YRMSLSTFLR FAVDFEITVV LLSKVQLIHS FKRSKFGAHD EPIDSNPHTR IKLNFQEWLD CLA RCAMLA YDYKPPPVIV PTLDFKACYH TNAKQMWLAE AVAGHHTGRG GLVGDDDELD EASELEMPAA VDYALGNEDR PHYD EVMSH APSIRITRSA GPGAGPGPHS PGAASVYGRD RLISRAVSIA QGRTRTQEPG GAEAMLIHPE PSTMQQDPNK PVHLQ NFTC DWVGKPGLTL SETGRLRQRS SDPFIAAGIY HTQVGVDPAY AARAAEMEAA RLVREQHVEA LKLHYQAERR QEIRDT TKA AAARRQDLEL TSGQALARVM GSLGASSSIS GGGRARSGRR SVSPQEQDAT AAADGVAAMT GAVGRRQGSG GGRRSVP PQ SMRSGSAPRL SGALGSGGGA GTVDGGGAGG GTIVPGLEAV MARTGGRSAL HAATTGSVSP AKHSRTLLQE LQQALDVE S KLPQHATTIA GHGQIRFG

Macromolecule #6: FAP297

• Macromolecule: Name: FAP297 / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 99.187336 KDa

Sequence

String:

MPPLPTLWDP AGAVDKLPQP FRMIDKILAD IVEQVVDMIG TRESQRRAED ASRVDLTFAP HAVMEVAPET CCFLPIGIAG IAAVAMPDG EVQVRSARDP SICFSDRTHT SPVTAMEAGM AVCTSGRLLA AASRTALTLH EVDIKTCEIS LLATVPLPAD P DDTSPPVR LHWSDNLGHL AVCRRSGALA LFTLSLPPPN VSLESVGAFV ALSLKAFGET RVEELLRVPA ATVRAFCLGS AA VGPSNLV WQLQQRPDKS PHSDRYHKSA RGAYVWWEGA NRLLLLDFEA AAGAAAAAGG AGGAVVPPPE VEAAVKAGAA GTS KPSTPA TKAASSKSVA PPGEASSLLT ASASAAAAAY AGPERPPEVP QIAPMARDWL LPHDVTAAAT TSDHKTMAWG LADG SVVIW DDRSCCSTKV LPRLKGGITA LSWVNGVAHK LVCASAGGHI FIADVIKPED SSQKPYEFPQ AIHEVHTLPN EPFAL CICR GHTSSDGEIA STHRGGRGGP SILSTMSGHG PGGGGHDVMR VPPQRPRVFW YNVLEEKPVA ELMGPRAEQG FGLACC VPP PPSLAPPPPR PDTAATDAGA ADGKSAAASA AATPAPGAAP KGKGGAAAPA PPSGGGGGGA APAASQEAPS EPAMSEA QR ALIKAALDAM GANSTTPVLV PVKLHVPAGQ VVSYPACVFR DTYLLAGGDV VDKVVRSNFA DDDAVPQRVT QLYMYKVD A LLRHLLPEDE STSRLGKVVL DRLLADLEAP KMNRKKGKRV KMDVEDPDAP KLSSAMRKPD PFDTTGSRPG SRAANRHIT FGGGDADGLF DDVLEGGRAK PKKETKVFPK ETKKGLKTGP GDVKMIDKNA SGRPRLAPLD LEKAKEAPLP FSERSQSPPW HHTNPLARI HPDWEEAPVL VRIMDRIGSK GGGRKRRDKR LEALTTELMT KYSKEAGAKP NLLVPT

Macromolecule #7: FAP114

• Macromolecule: Name: FAP114 / type: protein_or_peptide / ID: 7 / Number of copies: 7 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 31.575461 KDa

Sequence

String:

MTQASLWTHF TEDELKEYIS KLAEPETAAA FLASVLKLDN YRFDARQAIL LDYASYALEF AHKQGYGPTR TASWYNVAHG VLQTCISGA AYTECEAHFK GLMLQLVRPR PGTDTPHFSP DQVAAAAAFM ARGLLRHYRL YQHVFSTAQA HTEYTAELMV E TPVVPSFE AALGQADWAA LHDQRRAEAE AARKAAEEAE AARQEAEAKA AEDEARRLEE EARKAELARK PATLEEAIEH LV ATRLENE KESLAAAYKA KEAELLAKIN SLEEAAAAKK PVSAVGAKK

Macromolecule #8: FAP305

• Macromolecule: Name: FAP305 / type: protein_or_peptide / ID: 8 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 47.031668 KDa

Sequence

String:

MAGLAELQRV AAPALIAEYF SKCRLAEDRR DVENKAIVVI QSYARGFLVR RRLQRFIMTT VTIQRYWRGY LGRLRARRAL EEYNRALRK AYFNAMATVI QRWWRGFYSR KHIHDYYARK RYLKQVKERN DHIRAELNLE AERAIIVQRQ EAEERARRLF A DKVSKLHH LVSTAAQPGI FNSPYSVATG TLPVIMSLPV EEHLKGAMRA QLGPELDWLS SSAGAAGGGG GKSGPMLGAG SG RLERPGG AGGGGGGGSP STTGRLPPLR GDGRTQPGPG GASGGMGATG GPASAGGSLR RKSTKLAQEE IYPPVAVVAG RVV PARYTL RQAADYEAVR RQELLEDKIH RGEMLSAHPQ AFTTALGPPR PPPLDSQTNR NLVPFADPYD PTVGLRGPRF TPDQ QKVST AAFTRYLKRK PMFDKNLATE AY

Macromolecule #9: FAP119

• Macromolecule: Name: FAP119 / type: protein_or_peptide / ID: 9 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 33.850367 KDa

Sequence

String:

MARNLVWKDL SSAQLESIRH PHPKKGLTGR DLLAKYTGLN DYQTNPKTAI HLDLYVHTLQ FGDTLKFTDD KLSGLFSIVK LVHHRSIDE QLTMERSFLL FKELLLAHSV QRPPYSVGLF SFMEMQKVMD WMLDSYYRHY KLYMYSYTNR VTLSAASAHP S DVVELAPE LPPLQEALTQ EQHEAILTEE ERKREQEEAA AAAAAAEAEE AERQARLQEE YEAAIPEEVK DRVASAVEKE LA RLQKAME EQFTAQTATL MAKIATLEAG GGPPAPAAAA AAGAVRPPSS QPPAAAAAAA VKPPSAKPS

Macromolecule #10: Calmodulin

• Macromolecule: Name: Calmodulin / type: protein_or_peptide / ID: 10 / Number of copies: 12 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 18.317215 KDa

Sequence

String:

MAANTEQLTE EQIAEFKEAF ALFDKDGDGT ITTKELGTVM RSLGQNPTEA ELQDMISEVD ADGNGTIDFP EFLMLMARKM KETDHEDEL REAFKVFDKD GNGFISAAEL RHVMTNLGEK LSEEEVDEMI READVDGDGQ VNYEEFVRMM TSGATDDKDK K GHK

Macromolecule #11: FAP101

• Macromolecule: Name: FAP101 / type: protein_or_peptide / ID: 11 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 85.796789 KDa

Sequence

String:

MDVDNEFAAP AHAAADSAEP VEPALPPAKR LAVRPRYLSH GWNHPPGNLV AVYIEACERH KVPANSAVIS QLQRRVRLMQ AGPWQRVAP SGPEADALLG ATGVSAFSNH LGSPRSRLGA AAAAAGDPLL AQQSSYLSAA SATAAEALSR PRTALTSAAT T QDLLDLVV GHVYPLTLSA SLRPATANGG ANSGAPASTS ENHAATVSAA AIALAAGGPG GQALYAARAV AAVCALLDPR TQ WESQLAA VGLAPDVRVL TESQFHGLVE GAAAALTQGE LRQAALEVLG RPVGHGTLDV EGLCGALFDY LDVCGEGFIP ASE LRNVVA AFSPSAAVDM YGLLTQHPQL GRPAAAPVAR DEFVSVLVAL APGLVPAGSS AGRIRDKTEI VINTLLDSKI REPF GYDFS RCYLGPRGLL PVLDALSTDG SFVSLSLAHC GLGCSSVERL ADWLAGHPTL TSLDLTNNLI ADRGGLALAR LLQNN TRIV QLGIGNTMLL PPRSSRTHHD QLGPSPCEDA GPVLEALTAN CAAHRSSPAE IVRALRQHGP ELRAMCYSLL ARPVSS SSA TRSGGGGVNG ANGGSELMAA DGRVPLSDLR EALTEVTAEW GFTPDALEEV LSHERLLGTA TAGRTADASG RLGVTYE EL MHFLSSDDQV VRVARAFRNH LAEAKQLFFR LAPDPATAAA VAPAGVAGPR TALSTVRQSL MEAAAAPGSG WDVSEGDL E AVLSPAVLRK HYRAAATGYG GALAVAPPGA VLTSTGLTVG ADGLLVPEWR VAAAAAAAAA GVGAPGSGLG RDEDEGGLL PVPEPWQGPV AAAVAADMLI SWPELANTIL YLFNR

Macromolecule #12: FAP15

• Macromolecule: Name: FAP15 / type: protein_or_peptide / ID: 12 / Number of copies: 4 / Enantiomer: LEVO / EC number: protein-serine/threonine phosphatase
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 34.888031 KDa

Sequence

String:

MDIAQVDEII ERLLDVRNGR PGKQVQLAEN EIRLLCLTAK EIFMSQPNLL ELEAPIKICG DIHGQYSDLL RLFEYGGFPP EANYLFLGD YVDRGKQSLE TICLLLSFKI KYPENFFLLR GNHECASINR IYGFYDECKR RYNIRLWRTF TDCFNCLPVA A LIDEKILC MHGGLSPELK SLEQIKRITR PTDVPDSGLL CDLLWADPDK DIQGWGENDR GVSYTFGPDC VTEFLQKHDL DL VCRAHQV VEEGYEFFAK RQLVTIFSAP NYCGEFENAG AMMSVDETLM CSFQILKPAE TKAKGRR

Macromolecule #13: FAP227

• Macromolecule: Name: FAP227 / type: protein_or_peptide / ID: 13 / Number of copies: 12 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 19.550742 KDa

Sequence

String:

MPPKVVEEPP PEQTPPLELE GTGKFYYPSG AIYEGQWKLL NPPLPPPVED PKDKKKKPAK DEPPPPPPEP PKRVRHGKGV YREGDYVYD GEWFEDAMQG VGTFTYASGA SYSGEWLANK YQGKGTYAWP DGRRYEGQWE ENRMHGLGVY RDARGHKWEG Q FFNGSGPG LTCQL

Macromolecule #14: FAP105

• Macromolecule: Name: FAP105 / type: protein_or_peptide / ID: 14 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 31.382559 KDa

Sequence

String:

MSGVRACLQP NEGPACPIGQ TYGEVGGSSP NFRGNFCDAG RKHVSGPASE LATQGFSRWE QSSGNRDPPL RRHAEQPAST SYAEPSVMG GHASYYGRRA VGEGEDGTAY RRTMKAVPQP VRQDRPEGKK AIPEPYGAPP AHPRGTRPPP DDVRFREAES A PEPNLPRL GRPDGISGLR ESGDQQYQFE SSLGRKIRVG QDSYRGAGRA GDRSLVYGAP ARTEDDPTYF RSMKDSPTFT RF CNSLPAK PAVSPHQRRE EGMRRAAEEE RRREAALVST LDIQGVPDDD

Macromolecule #15: PF6

• Macromolecule: Name: PF6 / type: protein_or_peptide / ID: 15 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 237.723016 KDa

Sequence

String:

MAPKPKKPEA APPPPPEPSP DDNLPQDIKE LTECPFEVTW KPVVSIVDYG VGADAKGIQA TLKRKELARY IVLHEAVLRL DPATPLGQE AKAVLDAHIA AGQGPETCTF GPELSSKLLA DKFARDQLAD WRVELHRRRT RLQAAQDDAT AAAAGLTAAH E AVKAAGEA LAAYHRGVEE EEARKAAQRA EEGEEEPPTP LDDEEGAAPV EREAPAEEVA LEQAKAAVVA AEVQVRETAE TA SRLAAVQ NEPFVCYQTY ILSGLTLSLA SVRKALEEGT PLRGVLWAKA AAPAAPADGA AAAPDAAAAA AAAAEPAPWY AAV KKAAQK ALWEDVLAQL VPLEVQVPAY TPPPPPEAEP AKGKKPSSAK PTAAKGAKGA AAAAAAAVAA AAVEAPPAPP EDSP GWDAV SEVLKTLAYN VRTYDEWRAK VKVYDASKYD EVPAPPPEPE PEAPPPPPPE PVLPPPPAAG AKTPSAKGGR TTPPA PPPP PPPPPEPEPE PTPPPPPPAG PMPPPHNLGY YSALLDGVPL ERVSVPLVMH ALLEQVARNM ASGEAGEDLT EAGGMA AAE MAALSALDGA LSALDTELQE AVFAPPKPEA ADYSLVCEVD GLAAAAAGLT TGWVRAAPPR PESPTSHLDA AAAVAAE PS VLGAPASEAS GGAGSVFGGA SYGTGPEGLF THSGTLDVEA VEAHMLRCMM LPGAQSFADA RAAAGELAAS GSFGMGTG L DALSKSLRRT ALQAEAEALP VATIERYQLL QAAVDLLPPS AKLSHEDAIL ARHWHEPLDG PRLKQALAEA VTELPSMSS VYYGPADSVV VACYAAENLR STAADLPVAM NLSAFVRRAA AAAPGPVDLP AKVYKMAPEL AAGVEAHQRT AFLYPQTACI VSAPMPAVT QEAAAESVPP GVQTAATLAS LAAEGSAPQP TGSLAISRGG VALRWREGSG APADKAPGEG ANDPSGAPLL L GTLPGGSV LSAFHVVDPA AAEAARLAEE EAARLAEEEE AKKKAEAEAA AAEEGSEDSG PAFKPMMSAA EALKAGNRGM FE DEDEDED EDYGGGGDDL NTLLNKDKKP PPVTALQVTT PSGHLIRMTT DGQVLVAPAD DPSLAPPLAP FRGVAVHGAP LPG ADLAAL RAPATPNAFR AVTRDGLLVS AARGAVARVL CPDGSVSERL DAVEGWLGPD GAAAARGWLR TALDGTRLRE PDEG SKPKP PPTPPPEPES VAEPEPEVKG KGAKAGAKPA SKDTLKKGKG KGKGGDDEPT PPPTPPPESR RPQPPPPPPF TLELP AIGS ATLTDPDTGA RVTTREDNVM VVAYPAGDVL VQDYDGTRIT RAADGAWSVE MDGFAPILGS PAGLTISPCP GVTLSW DAT TGVVTGTLPD GSAVLAAPHA AAFAPAGVQP PPLATLASEP EALDEDEDDE DEDEDGNMRK KPKKEDPVAE AVAAANL LD ELAANPDLSG IFLFDPRGGS CAFYESEQLR FFLLGPLARG AAQAAAKAKA GADDDEEDDD DGGRPAKVFD VPKPYWPP P PPLLHIHIPE LPPPPPPPPV VEEDEEEVKP AGGSRRPSLM DPDAGSDAED EYGNSRRGTA DGATGAGDGE EAAPPPPPR PPTPPPPEPV ITYLPVAPHI KPRIFVIRND GSGFEVLDTQ LVSSYLAGRR QMAAESTARP GSAAKPGAAP GAAYGMAVEL RREELHGGE EEGAVMHSVL VQVPQRPPAL EPLPVGGAAL LQRYRPHLEE LMPAFKRLAQ LVKPEPGAGL STAQYLPRVM S YEPPVVRA AAPLPVLVYR ELLELPALSG ETIATIREVL AQQARTEQTQ AMMYAQAVPY SDPRPSETAA QDADVLAGIL AM RSKPPIG RVRSVAAEVR RRRETSDAAA VAREQAARER EMATAINSEK EKRPWPVKVG PFDHTPKRPE EGMILPYFGS EEG ILALEN DPIIQQPINR RMLPPRLPPP AATPGAPSTY SPGVYVADRP APPPGGSDLG AGAAAGVEYS SPSRLGATGA SIMS RTGGA AGTDFAYPEL AHSQILAQHT ERAGPGPSLK SHAYDVYGQP RTLPPPVPRQ HRADEAEAAL NESYLRAEAD AVRLG KTSS TQLIRAAGKA LRQFALTPSH LHLGTVPVGA VAHRTARLAN VSTGAARFSV VRPELPLRAI YKPGPVAAGM EALITV EFV AEKVGDFVGE VTVKSELNVL TITVSAKVVP AQDGGDEAAV AAHDAGSSPA RRGLESAGGG QLPPVTSGRK SASQSAV GS RLTSPLAGAS RGASPAVGGH NVGGLDIPSL DETKTLNQVI HGDATGGAGA EEPAPEPAP

Macromolecule #16: FAP219

• Macromolecule: Name: FAP219 / type: protein_or_peptide / ID: 16 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas smithii (plant)
• Molecular weight: Theoretical: 29.104484 KDa

Sequence

String:

MDDTGSVIDD LPPPSPNRSL VATPTPAIPG RTGKLDYGLH ESAMRVPVLD KVKEARKATI ESKPDILGVR GPVWNETVAL NPGKHHGKF SHNLLSNTLT PELINSTDVR KLTGTTAARG DPAAATLDRS LSPAAGGPSG WNTSTTLPSS NDRQRQLEAG L NASLAATA RRRASPTPHY VDPVARQTAY SETIRAIKAN SGADMGELTA RYGPDGAEAM AAILAMPAKE SRPRIRTTRA DL QAVAALD AFSAGRDDAE EEEQGEAVPL SSPMPGAER

Macromolecule #17: FAP99

• Macromolecule: Name: FAP99 / type: protein_or_peptide / ID: 17 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 90.340258 KDa

Sequence

String:

MESRPGSASS YHSHAAGNTQ SRPGSATSVE SSNRLSGKPL GAEKLMAACE KIYKSFNPAQ VTLDTHVDNC IGQLSVHNSF DDSFIRQVV YGTVRYRRLL GALMDSFYHY NGGAASREDV DMYKLYSYLT IFRLEELNFS NFQRLVDAMT PQKMYVLLKY M FNPQYMRE VVREDWLKLY DKEFVDEIID RLLSWKSESK EMLGRLEYKV TLSRKEDDEK ETLGTGYAAA RSTTVPQPFN LT QPKPRPL PVEDPPPPPI RAKPAPRPRE GPTKEEVALA AAREANRAAA ERKAAKAAPF KLRVLERPTN IDKIREELEA ERT RELTFK GIRAAPPPPV PNAQVRLNAA AILREDALYR RKQQEEADAL KRYEAELRDA SSFKAWQNAM LEQDEAARAA SVER RRQEM AAAQENAIRA RMAAQEANAE LARAAKEEAK RIEEDLKRDR EEQARLNALR RDAVVEARQN VQAAVEKMSQ ERRLA AEEE RRKQQEDARA RAEVAAREMA ERRDIILQLK ALEKVPKQRV KEFDPTETGP DHGLLETMSL VELRERLNVA KRRQRE EEE RQRAEILRQK QERESALLEK AANIQRVRRV AAAQAAQRRA TSAETIQRKN TEVSKAREAD VLQLADKLDA KRAALAA ER ARLAAEQKRT RFEQMQAAAG AAVVEETKFR ELRAGAQREA KTRQENALAS ATVYEATKAR QQNVRLKNVR QELKAKDD F MRAYDEKLAA LRGQAGAESA ADLARRTQMA QTQRAAEATV RNRTTTTAYR PYEGGSTSMQ ARLAALGQGM ELED

Macromolecule #18: CPC1

• Macromolecule: Name: CPC1 / type: protein_or_peptide / ID: 18 / Number of copies: 5 / Enantiomer: LEVO / EC number: adenylate kinase
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 205.400188 KDa

Sequence

String:

MSELLRKWLA DELGLPVTDN LEQDFASGFL FAQLFSKYNL QPDVDHFDTK RMPDAMINNY TRLQPTFTNL GVHMDTRVAN MLIREESGV APRLLYSIKQ NLGSIQKTLT KHMHTGNLGR TLGASVSPSR GLLEAQKHNT SKEKFESGTH RHFEDTLRSQ A ANPNAIME SMALAKFTEE GIRQQREALG GLLRDRAQHT AQRDTFRANQ MDKLGSARSD KAQRLERDEA VHAALLKRKE EM EREELRV ELALSEKARR RKLEELHHAA LDVHGGIDAF EINMKRLVRG DQGQEGEEVV APPAGRTPLE HMEQLKSRAA ATA KLLEDT AAYMRGVKDA RADEVASKRE REARRRKMVV EQQAAASSAE RKAQMESLLT ALQRQSAEEQ RLAARLWQVS QEKE VMREN RLLRERQYAE RRERDWEETL RREVELHRSM RETYEAEAAL EMEAWRAAQQ ARAEAKSAKH AAFARDVAYQ LVSLA ERSA EYRAATGNLV PRREWREWLT MFVAGDPQLG PPAPPVGAEA EAAEAEVREA CLRVGRALVD DYLACAGDWL RVDGPI GHD ELLGQIVGEL AVLARQPPPQ PELPAIETLP LRLAVLGAPF AGKTCVAQEL SRRYKLRVLE PEALVAEAMA AAQEFEA AT GVPADQVPEA APQEDGDAAA AAELQAGSAS RPGTAATAAA GPSAKARLGA QIAAALKAGG DVPDEALVGL VVLGMKES K DWAPPVEVDP KAKGKAAPKP AAPPAGSKSA GAPAAPHPDT GRGFVVDGFP RTAAQAVLLE RMLTGLDLDS EQALIDAAS VIAPPPASAL PQVARPLVSG LDAVVVCGLA DPELALKRAL GRRLDPQTGR VYHLEFDPPP SNDPGLSARL QEVADASNDA QQIQHRLMS QAELAGPLDD WLRRFSRLRR PVDGSGPLGE VLASAGDIAE GLLRAKAAAA SCRSAAEAAH KARASAEQAH E FAELAQAA AESAARELLT AKKAEIQAAA LLAGGKNPDP AATEVLKAQA AAKCAEQLKV ARGAVADANG HAERAANSAA AA SEAVDRA HKSLGDAEIS AHAETEAAAA ATEAEKAARG AQEAASKALA AKEAAAVAAA EAERFAAASD LPADATVNLG HRP DAHATG AHAVSTSGGA PASGNGTASG EAAAPAPEPL VRELAASLHE EWKTLETGYL EGLALGFASL AEQHAEAQRH FAGL RGRFQ DLLQRPDEKP ALVARFLTTF NAVEADLRGN KEARGELLLR AEELRDALWA LCDKKMEEAE AQRAAVASDT FVPDH CATL AQQFVALAQV EGDRFAAALN FLRSHAHSKW LPLFPPGREP PSLALPDVAA PDLLSGAVPP ELKDKIDPKA KTSVPM PAW VASLEQRAAP LALATKLLLA LAKTTATSWE PGAEDPKKAK KDAAAKAGAG AKGKGKGGEE EAPEADPRVV EGLNGEL LD GCGRELAILE ARLQLLAERC LGQMDEMAAL ASGTVSRLGE WVRERYRSEC AAVAALDKVC KAAAAAGQPL PHDLRLQD E FLLLDEGSLL VQEDAALPRP YPREGVPAGG LLSTKQLGAL TAAFLSAAPS GYMRLQEAAD MLCRLAAEGA LPEPWRGTS VASMLGALRL YDPLHSTYLD WREFVAHLVV ASFPLIARAD CAEMADQIDI CAEVDKDHDG LLSQEEWSTA ELWFQYRAAQ PGALQAEVP EASGEAGAEA GTGSGSESDA GPRSTLLADG EKWPEADTVS RAGTPPPDDE QYDSPAALKQ VLWALFSAPA A DGTPRLDY RACLLYLCAD RDAFAGIKKA FSVATANISN NARASADQVF RIAYPLGAEP GQELHRSPFS KVDVAAVVKA VW ESRAGQP AVAAGAAGTG PAGARGATPP QAATAAARGA SAKNTAGAAA PAASGEASVT AEQLMYSAGG ERFVKRMLER YAW KDAFVA TRL

Macromolecule #19: PF16

• Macromolecule: Name: PF16 / type: protein_or_peptide / ID: 19 / Number of copies: 36 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 54.71059 KDa

Sequence

String:

MATRAVLQTF ERYQKERVAF VTAVAEMAKN PQNIEALQQA GAMALLRPLL LDNVPSIQQS AALALGRLAN YSDDLAEAVV QNEILPQLV YSLSEQNRFY KQAAAFCLRA VARHSPELAQ SVIDSGALDS LVTCLEEFDP GVKEASAWTL GYIAGHNAEL A QQVVDAGA VPLLVLCVQE PELSLKRIAA SALSDISKHT PELAQAVVDA GAVAYLAPLV INQDAKLKRQ VCCALSQIAK HS VDLAEVV VGAEIFPKIL TCLKFPDEFV KKHSATVVRE VAKHTPELAQ LVVGNGGVGA LVDYISDSAG NNRLPGIMAL GYI AAFSET LALSVIAEKA LPPLVSALNE EPEDHLKSAT AWTLGQIGRH TPDHAKAVAD TGCLATLVSL ESDGASSDDL KTKC RRALK SVIAKLTHLP ALDALVHRQL PESVMKMVLE QVGKVLANDA AGRAQFVHSG GLAAVQQMAE APGSKLKEAV EIINS CYPE EIVKYYSPSY SQQLLEKLES MAATTTAY

Macromolecule #20: FAP108

• Macromolecule: Name: FAP108 / type: protein_or_peptide / ID: 20 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 47.789062 KDa

Sequence

String:

MPLYFEEVAP DPKAKKERDA KQQRPAILVE RKGPPPAPMH LESQVIPTLI RKVGDWKTGR ISQAMCEAYL DRHTLVFDRE LLTKLFKEA DYQKEGSLDT RALTIAIAGR FPKREHTPEW RLLTALLLGL PELVLTTDAE VTTLRTTHER PVGGGTYNSG N FWDSPPPP LPPVRRRTGS GRSTVGKVTA HEPSPEWLDT LNRTAAAASM SAGGSPSASM AGSFAGAASL NASMLRTGSV GA MDPGGAG VVGTTGGLKQ TTQIADEARL NAALMGGAAS TFATQREFAD WSRGLEVMPR LAADTAGPGP GSEFGGGVRT ATH LGSPKA PVRVWAAPLP PSAISLPSSA LRTLRETVRS TASTKPDFVK GVKPLDSHEL DLKKTLGEPL DVGMSLARVE PVRD TKVLP NADYVTWGDY AANCRTGPTG WYSKHPTAQA QDTGEHKYPW C

Macromolecule #21: Hydin

• Macromolecule: Name: Hydin / type: protein_or_peptide / ID: 21 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 528.934062 KDa

Sequence

String:

MVAGSRGGSL RHQESPPKAT IKYKKQAGGN DPTTQAKKLP SQQVAELSQP VKDVEGWRPR PRIIEHLNIA DFSHHVDCKV PIDEPIFQP FPPEVTFHNY EPFQTYEATL YLRNNDNVSR RVKVLPMDSP YFSVRRAAPP AAGKEDNKVA TGMEVAFTVT F KPESRDDY SCDLVVCTER EKFVVPVLAV GASAALDFPD LVDFGSVATK VETKQTLFVR NVGSKAAHFS LSAPPPFTVT PT AGHLSPG ETLQCSVVFE PPSTGRYEGE LEIQYDSGRC VYAQLVGAGH ELEVGLSQGV VTMLSTFVTK SSQKTFKIIN NSD VSVKYS VKQRSTAEQD MALTAQRLGT LTATQIGGGY HSGGAGADNG DGDASSEDED TILAAAGPQL TRRLHKAQRD ALLD SYLFT DRNFSVNPAE GTIWPRSEVE VVVTFSPDHA REYEVVAYVD VAGRADRLPV VFKGRGLGPA AVFSYDVLDV GDTWV NTLH QYEVELQNRG KIDVDYRLVP PGSAFGTRFS FEPPAGRLSG GQIQVIKVKL LSDLLGTFDE TFAWQIKGSS EPLSLQ FKG RVCAPSFEVD VEGLDFGVCS YGFRYTKEFT LTNTSEIPLR FAWRVPSDTE EPKEFQILPG KGTILPHGKV KINVEFV SR TVQRYRTELV MDIPGVAERQ LVLPLLGECA VPKLSLYSRT MEFGECSLRF PYKQEMRIVN ESKLPAKFEV VPQDSASL G LATFTVEPSS GGIPARGEQV VELTLTTHTL GRIQIPVKVK ALGSKAAPLE FIVNAKCVGP QLDFGDDPDN RTTTPSLSF DKVPVLKEHS LPLVINNCSA IPADFKLFIE SKDSVFSVEP RQAHLEPGDS LTAHVMVKMD ESMDFADTLH VLIQEGADMA IPLTSSGVG SAIVAEEFAS GLLDFGYQFV GRPFTKEVTV YNMGRKMVML AWSSSRYEEL KKEYAKANRG AGKKFDIVLI P QEQQPVFS ITPDKVQLGP KEAATFTVTG NALAAGEVRE ALTCNGTFGN NAKGTKKVFE TTMRADVATP LLHFSDRLMH FR YTYKKGA AIETQTRPLT IKNVSPLPLT FGLRPQPPFT VDRTSWTLDL EESGTVNVTF DPNFKDDLQS MQSRTKLSVV YSD NPQRDS VDLHGDIDFP NLAFETTTVD FGSVLLDTTR RVPVRVVNSS NVDVVYSWAW DKSSVQEDVN SIASMSLRQG RPKP PPTQL FDVMPIRGVL RPGEAETMTF SFFAYPGMRA SCSAVCQVEG GPLYTVQLAG ESNNIKYSVE PQGMDFGQQL YDRAV EREL TLTNSGKVPF TFNFNYSRLS RPGIVEATPS SGAIAPLTKE VIKVRVCPGI PEKLTETLLV EVAHFEPIPL AISVEG TYA AVSLNLPRHR DETFVDCLEK ARAAIIAAAL SHVPADEPNA PDEAAMSLAG GRDGTGSVAS PTRPTAAGRS IRGGPDA VG SRSIAGARSV AGAGSRSLAA SKSMARPPGT AMQRAQSAHS TAGHGGAQLS SEKKIEVEAE RLRLITLLME REAEKRRQ Q EQANAQPGLP DAVAAADGNR GGQPSPGALA ALQRQHGNAD GNQSPTASQA GDARRRRASG GGAASVAPSA LRRQRSNNS LAISTVTGFA GRAPAAVVDP LAPEEEGRRA RPLSLQPALP SLAVAHYVLD FGYVVKGLTR TRKFKLTNTS NQQVSFRFDK TLLENNGFK VDPEVVSRLP GAPEFASVEV AVTLQANKST VQPGLLELSY PITIKGSPPV LLTLKAHVQV PDLKLSTELV D FGACQTGQ CKVFTVQLHN HKQVPCEFAI KKPAEVIKAK DWQFFVCEPS EGTLEPDQRM NLKVMFTPIL NRDAPYAQGI PL KINLNPR MKELQASGRG LTPRVIFSPT FVDCGAILPA FEGQEPNEAK VIMSNPCAFP IEVVSLDFDP RYSRDEEALR AME GYNDNG VLFLPPLKPG DTLWPEVAEA AAQKKRMEAA SAEAQAAQDA AAVSAGGAPP AGAGREEEGV GSLEAVAGAG PLGA HFSAG NVGAGPQKVL AVVTGPSKVG ATTQAKLLGT RYGVPVSNLD DLLMAAADLD PPPEEPAAAP PPALMEPMDG EPGSD GEGA PPPVESMEAP PPPFDTEIAD LLYDHVLSDP DHEGKPDYVP PHTKLKQPEL YGLVVRGLRH LLVQQPVAYG KGLVLD GLA SRYLPPPLVA KALLEALGME QVMPPPPPPP AEPVADAKGK KAALKKGEEP PPPTPPPAVE PLGWRGPVQV FFVQLSA SP ETLVTRSRLA AVEAAAALDG AAFDLLVPTA SVNAPSAPAA AAGALPQGSR LGTPLMSLTG EAAPNAAGPP PSTPPPAT S SGAAPLVDIS PSPVPGEELP APVPAPALDE AGKRQVEEAA RAIADYEASV AKLVRELGPA GPENAVLHRS VSAEGAEDK VHKLAVGMAF RMGVLSTALP AAPCDKELVP PRFAMQIVPK PRPRAARTPV QRFKVFTLID YTKEGAPPPP PPPPPPPPPP TPPGKSRGK GKEEPPPPPP PPPPPTRALV SDTRWVIPAG GSVELLLQFA SPEVGKFTET LAFDVLGGER LNTLVVTGTC D YPHISTDY RNVFYKKVKA RPQTPLIRGQ YIINKGQFEF GPLLHSKDAT GYLEGSHPDN TARIRITNNG LFDNHVEFSL KS VEQARAE EEAAAAAKGG KKPDPKKPEP KKPDPKAKDG KGGKGGKGGA ETPPPGLTLD NVFTLFPVTL DLKVDETQEL SVY AFPVDD GLVEDVIVCR IKDNPTPVEF PISVIGAKPQ VQVRLNDGTP PPDPAAPPAT PPPNKLLTEG IMFERLLVNK KDVK AFTIT NPGLLPIKWR LANTATLPKE FTVYPQSGEL AARSDVVVTV EFAAVEKRLC EAKLALEVLD VQELQGVAHS IPLPI KGEA YKIEIDINFP QANFPGVDYG TLRVVDDVAK PIVIKNTGKY AISYNFSMKP LSVLTGLLTI TPASGNIDPG KDARIE MAW NKDKSLRNEV MLMGNSDVTL SIIEPLTTNR EDTIPIQISG HAVFSKYAVT PARGIHFGPV TYNTASKPKV VEITNLG QF EFKFRLFNHA NGPPPPIPVE APAAGKGKGG AAAAAPPPAA KGKGAPVTAM TIGQFTFDPP EGSIPPGGRR EVAVVFNA V NASVYSEVLG IDISERDFGD QPAGIPIEVA GESCIPGIDA ENTVAIFEEH TISASLDPFN PINNEFSTRE RVFNFGAVL AKLGAPEGSD GSATPPRAST PPKGAAKGKG KDEVEASPGV AAAPHAVRAN LKFINPVKVP CVVNFSIKPR GNLPPGQALP MEISPSQLV IPPLEYRYTA LYFAPRAIQS YLATFEATVE NGGDPKTKSF TCEVRGEGTL PTLTIAEPTV VDPQGRPLVK F ARLLRGRT ATARITLKNN GILPAKARIE MAPHGSFRLE GGGATGSMGP QGEAFTVESK RSVTYTVAFT PEAVGPAAHE LK LRVANNP FEDYRFLLTG EGYQEDVTFD HLPNDALDEL RLQDGPVGRP VQAVFTLRNH SASKHFRFRW PAAGAAGSNP CLA FSPAVG HLMAGSSKDV TLTFSAEAPV KLSPHDVKLT IAQITHAAEA HAVDWDDRSV VVDYGLGVGP DGLPVAKPEP EPQV TEVAG SGRELLLHVF ATADNARYTS EAGPIMFKPT MMFQTRTYTF PLTNTSTARM DYKFTVTQPD GNTADASGLY TVSPA GGVV EAGATATITV KFSPTEVDDC ARVLTCHIPH LDASCEPFAR PLGGRVLRPW AHFELPDSDY LSGGRRSPDM PGPSGA IEP LDPGTRVLEA ESLGVRVRNT KRFFVLNPTG ISYEFFWTPV NKAQEAGPFT CTTLRGVIGG GRRFEMVFEY TPATDAV VE SFWTFRIPEQ NIEVPFLLVG LVSEPRVMLD RPSVNFGQIL IGCKGHATLT LVNNEHLPFQ FAFDKNSYDA TEELIKAT G LRPVVELEPS SGVVPAQGSV SIAATFTPRL ERDINYNLLC TVKNKPTRLT LNVKGEGTAI HEALQLENAD GTTVTMAPR QSNTVDFGQV LVNERCVRAL ALVNSGQVNF NFIWDVGTNP RVNISPEGGT VPRGERLVCE LSYNPHGPDR LKDYKVSCQV LNGPKYTLL LNGVGHKPRL DLSWFNHDFG LQPVFIPGMT PAVKALRLRN DDAQPISVDP HWDANAPSAG DWQVDCGALV L QPGESREW VVTFRPRGAA VCAMSLPLEI NGLYTVHVEA KGEGSPLRVE VANPAHRAVN FGPVAGGGSS TRVVPIINRG RT TAVLSLA PSAELLARCA VDVIPAPTTE LLLRPRESAD LTFFFKPQAR MRPFTEELVV NLCGVPTPLA TLTGACLGTE LRL ASDSLP FGPVVLGSRV VKRLQLENTG DVGTKFVWDT RALGSQFSIF PADGFLAPGQ DVKLDVTFHP TEVNPDIRVD KIRL KVEGG ADSTLTLTGA CVATAAQPEV VNFSCNVRAQ AQQTITITNS TSSAWALRPV VANDFFSGPE SLSVAANSKA TYQVT FRPL TMSTPDRPHE GSVFFPIPDG SGLLYRLVGR AEAPVPEGKL ERQIVAKTQH TEVLKCHNWL HKPQRFRVLV ERKGGD KST QLSTPEYVDV PPLSSKDIKL GVYSYTASTT LANVTFKNES SGEYLLYELK LVASPPASRG TLSLECPVRT QTSCKVT VA NPLPEDVTVK AASSNKQVVV PATVVLRANA STDVEVRYRP LVVGASEATL KLESAELGLF EWGLRLAGTP TNPERSLG F NVPLGGRETQ VFRFTHWLDE KADYKVSFKS SGTNTATGGA FTAPPTVSAT PAASGAGQAG TEASLEVAFE PTAIGENIR DMLILTSATG GEYQCPLVGR CIPPKPQGPV DVSKGSAALP FSNVFSADAD FQLAVDNPAF QVKPVERIGS KKASNIGITF KPPDAKAGG GKGKDVAAAE VPAGPVSRTG KLTISCPSQT SSQWVYYLQA

Macromolecule #22: FAP221

• Macromolecule: Name: FAP221 / type: protein_or_peptide / ID: 22 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 103.833531 KDa

Sequence

String:

MFASSGLPPA MEGLVEDAPL QNPNTLTYNR VYSKVGNTRI LQAEPAVLNF GGYELGKVYS QVLRIRNVRA SGTRFHIIPP STPFFKATC PAKKGLLAPG MTEEVAVEFC PTQYRYYYDC VRVHCEEENL LIPLHAYPVA NEALFPTRVD FGRVALGQEV V RSHTLECK VPVDFEYEIV EVKPNPAFRV EPARGVVPGR GRVTVDMWFC PLALTTEEAV IEVRIAEFNS KPVQCRLTGS AF PGAGRDA ALAATTAANT AAAAAVAATL GRGERTAAAA TANGKHGGGG GATASGLKGA DLATMEALEG GLTMQLAAKG LPV NPGGGT LRGGAGNGDA YTVAVMSHRA EAAAARDCRR DGPIRSKPPL LPATAGETEV AGVYVPDTML LSAAEVGYVL NQSP GRLRT KDVRGAIEAR KAAAAEQQAA LEKVLGGSAG GGGGGRAGGG GLGGIHPLER PDIPADVKGA LFRLQLALAE EAARK VALG TAPHSGQQLL TAEQVRRMQR QRQEQHRRHQ LSEEKRAARR LAPELEAGGA VHQPSAPVGS SSSGGGGGSD PAFKPE WRL IEGSDWHKRT RVLDKFMRAV WRVVTHQRLQ RRLERIKEVL AHLGYDKQRL AEEAANPVLL VSESDRPGTA PTKYLRP EM VRVRPLPLYR DVLFQVHHAT DLSHYTDFDE LAPFTSKVPL EYRLLGYAPE PLPGLTPYLP PLADQPLLEG AVEEVAAA G ERLVPSGLVP PLSDYPTMPD ACKNMPYITL EIGNRYGDDR VYGAPDPSYS PYGSLDVDYA VQPRQYDVYD SARHEAVAS GGVRSLRGGP GLSDSWLVRQ DTWAVQLGAE VVPALMSGPG AGELPEDRPE DGDKPKICPA VPTDEQLAKY LPTSSRAQEL PPPPGTAAS AATTHTQLDA TTTPRGDPSA SPSSPSTSSA APTAYRLVRD RHVSDLEAAK AAAAAAALQA WERRLEDYNA L LRRPCQFA LHL

Macromolecule #23: FAP194

• Macromolecule: Name: FAP194 / type: protein_or_peptide / ID: 23 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 57.764508 KDa

Sequence

String:

MPTATAKLTA VAEVAGTLKQ VDQAFTAFQV ARNTFVNVLA GFLERLDDQN VMLEALMKKD VLATLCCPLA QDPVPGIQAS ALSSLSKLA GCDPLLSQAV VSCGVLDSVV LSMSHESSPV QAGANMVLAA VAGSSVDFAR RVLEAGAMPG ILTQLRVGTP A VKEAAVKN LNSLIGSHGD HARLIADEAL LSTLVAMLSA ADSPHSLCKA VVHTLATTAR YSSEMAAAVI KANALPPVSL LV RGGSTPP DLRAAAINCL AHIASHSEEL ASQVSATGLV GPTVGHLADK MVPKVRRAAA ALLLQLGRKT PPLAAEVCSG GCP AALAKY LAMEKDGGEG CSTGVTLGGT LASYSPVTAK AVVDAGVGAE VVAALRRGGA GGADAGGGAL GATGKSGGGT KLLT MKSTA AGGASAGVTN VPVPAAAAWA LEQMSQHGDE TTMPLIAQGA LTAVLDGYVA SADGAAGSPD SPDAAIFGRC KAALK SLIR NCATTAPLEP LVMDSTPPAI LKHVLRRCEP LLAKDPKARQ QFVTSGGLMR LQELEGRLCG KGRAYLEAIN KLFPAD VVN YYRQGGGK

Macromolecule #24: Tubulin alpha

• Macromolecule: Name: Tubulin alpha / type: protein_or_peptide / ID: 24 / Number of copies: 97 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 49.638008 KDa

Sequence

String:

MREVISIHIG QAGIQVGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDAFN TFFSETGAGK HVPRCIFLDL EPTVVDEVRT GTYRQLFHP EQLISGKEDA ANNFARGHYT IGKEIVDLAL DRIRKLADNC TGLQGFLVFN AVGGGTGSGL GSLLLERLSV D YGKKSKLG FTVYPSPQVS TAVVEPYNSV LSTHSLLEHT DVAVMLDNEA IYDICRRSLD IERPTYTNLN RLIAQVISSL TA SLRFDGA LNVDITEFQT NLVPYPRIHF MLSSYAPIIS AEKAYHEQLS VAEITNAAFE PASMMVKCDP RHGKYMACCL MYR GDVVPK DVNASVATIK TKRTIQFVDW CPTGFKCGIN YQPPTVVPGG DLAKVQRAVC MISNSTAIGE IFSRLDHKFD LMYA KRAFV HWYVGEGMEE GEFSEAREDL AALEKDFEEV GAESAEGAGE GEGEEY

Macromolecule #25: Tubulin beta

• Macromolecule: Name: Tubulin beta / type: protein_or_peptide / ID: 25 / Number of copies: 96 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 49.665809 KDa

Sequence

String:

MREIVHIQGG QCGNQIGAKF WEVVSDEHGI DPTGTYHGDS DLQLERINVY FNEATGGRYV PRAILMDLEP GTMDSVRSGP YGQIFRPDN FVFGQTGAGN NWAKGHYTEG AELIDSVLDV VRKEAESCDC LQGFQVCHSL GGGTGSGMGT LLISKIREEY P DRMMLTFS VVPSPKVSDT VVEPYNATLS VHQLVENADE CMVLDNEALY DICFRTLKLT TPTFGDLNHL ISAVMSGITC CL RFPGQLN ADLRKLAVNL IPFPRLHFFM VGFTPLTSRG SQQYRALTVP ELTQQMWDAK NMMCAADPRH GRYLTASALF RGR MSTKEV DEQMLNVQNK NSSYFVEWIP NNVKSSVCDI PPKGLKMSAT FIGNSTAIQE MFKRVSEQFT AMFRRKAFLH WYTG EGMDE MEFTEAESNM NDLVSEYQQY QDASAEEEGE FEGEEEEA

Macromolecule #26: FAP74

• Macromolecule: Name: FAP74 / type: protein_or_peptide / ID: 26 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 204.036094 KDa

Sequence

String:

MALTAEEAYL EELWREEDEQ SEEATAMQQM LAHQHEQQHD ALPSYQELMG QGSPAVTLRR AKAAAAANGT SSPGIRGSPS PARGPGGRL PSLAMGPTVG ANVEQLKRRL QTVVAEVEGH QQRYDKVLLE ANKATDLVHS MEAEIESLYV EAEELARRVP P AAERQRQV ATWLAQRSAA LEAQRQVLGG KQLELIEAEA ALRAAETELD FMAEARNEIA AAQAAETAAL SAAAATLRGK ED KAVAGYR RKQAAEAARL ADVAAAAEDL AQRRIQAAEE AQAQAMERLK ANTERIREGI ASGRDLLAAE QSRRREAVLG LKE SLAAVQ ADVAQQAELA RVLQRQRRDL QEKEFGALVE AGMNPYEVFR RRDQEAAAAR QHEAITSNIA TRQLEVAAQL AREQ EAYAQ KLAARKREKK TREIFDREMG PAAQQARTEA YMQAHTVGGV SMLDPTGRVQ PFPSEAVVVK TRRFGRGGAP EEVLQ QQLQ RLGDDTQPKQ LLLPAKYRNT ADTDAAAVAG LGRGGGSDDE DGMGSPPRSP SLTQQLAATQ QSLTATASLA QQQQQA AGD RYKQLATRAL TKHEQGLMEA AKTRHKGGIG APQTQMGRTF GGDAFLPSPA VVEFKDFDVG RTVSASVQII NRSYKKN TF RVTGIPVEYC DVLDFAYKLP GYLAPGVSGE VVISFTPKAP VDIDTSLQLL ADTGPFEVPI RCRTKRAALS VSPSAVDF G AGVTLGECAT RTFTIANDGA LEVEFRLDSP QLSEDERALL KSTANMAHLM TSATVAAAAA AAAATASGQG PRSARSFAN MGVADGAAPE TDTGPLVAPR EERRLAHGGF TVFPCVGHLK GYSKVTFTVT FAPVVAAPAK LLLHASYKAP AMKRLALPHH EVTLTGTGR DVPVYVERPL IDFQCCMLGH LYRDVLVVRN GGKSAMKVMV VPRPELEGFF EFSPDFGFVQ AGDSFPITIR F KPTPALLH ACRKHVVEPE EEQILEIGMR LSVPDQSLPV PFTLRAQITN TDLLFQPPAL DFGDCVMGER TAVKLLVRNP GR LPQTFGF VGLPSGLHFT PNDGFGYVLP GEALERLVSF QPPIAGPQTF SITAKTLAGR SFTLPCRANG VAPPVVLSSN RVR MPATPI GDTRTVSVVL INKTDQPQSY EFAVPADSDL TLSPHVGRVP PQSRLRVQID YSPRPPVDGE QQGALPPPSA PNTA RGPRD GGGGGAMANG NGSGGYEHYN DDGEPEDGEG DADGHTDTDS FQSPSARASP VQPLGRGGRG GRGGAAGEDE DEDED AGEL PVRGKSSSSS KASSGRRSSS PQLGGRQGLA MGRINEVPDD DDADAEAEAA AEAEQRTALA ALKRIQSMGG GDPGWY RWR EHAITCYIKP QSQNPTPSQS QSGQAPAASA PSDGASGAAA AAETAASSGP AAAPPPQEIH LQVATCAVLP DLVLLAP PD LPYVPLHQCH CLDFGPVPVG QRVVRRLELA NEGEEPLVLG AESMDSREVF GLVNALRPVR PGASFRVMVS FTPQARTE Y LEVLVLKSAR TRLRLALKGA GIAPELQLGP EGVTATGLDM GDVLVGEAAA RTLTVTNVCP FPLSFTMRLA GKASAADPN PTMRQAFSCH PAEGTLAQGE SCEVAVSFTP FSQRPYFEDV LQVVVPNQQD QLLVPLRGRG WREAVFVAGP DYPEPQPDPF LLHHLAKQA GVALPPSVSG GGAAAGGAAV AAAAAGDKPS TPAPGIKPPA TPPGGASKGK AAAAAAAAAA AAAAGASEPR E LTLNFPHA IYPGEVATAS FDVGSLKSTA SGSAPGEVGV AELPAEAREA GWAVTDPPTA GAGGGGKVPL AAGERKPITL SY SAPAAPH PGMVAAYGHA EYRVLKLQLT LKGGLAALSG GPEGRKVVVA ARCRLLPGSR PPGEPVPPGV TPAPEPVAAS GPG AGAAGV KKLVPPPSPP KKAV

Macromolecule #27: FAP69

• Macromolecule: Name: FAP69 / type: protein_or_peptide / ID: 27 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 114.362844 KDa

Sequence

String:

MTQSMGGAAA GATVELEKLI ALFTGPNSAD LYDRHVAAMQ RLCRNNAAGF AIRDLPKVQQ VLELSVALLK RGSTGFVEPL CELVGTFSK PFIRRTATDE FKMLNHITSL LMTVGDIFRS PGMPPQLVAV AAQTISTFAN AYGNRPNALD LASQHVEAEG P QRQYLTNQ GLLGKSGVVA DVIVALARSA LADREPPTSP SARGGAPDPA EQARALAAPL LTALLAVSYN AESCTAMVEA GV LQCIAAL LGRGPHADTS GATVELLWNL LEQAPALARA ALSRSLPSLE ALAAGAGLPV PAGGKASARA NGEEAAEQQR NGS QDGSAG AGAGASFSGG GGGSRSALPP MASGAGSRLV NGSRLGSPGG GAGIGSIPVS RGSVAASGLS GIAEGSARGD GASS DAGAG SEPASPSRGA GHDDGSDWQQ LPDGASAGSG SVYEAAVSLR ASSPGGASAL DEFGEEPDIR PLRGGGGLAM VAEES SGYG SEPPPPPPDA PVVQALADAL GGLLRELLLN GFSKADKELR NDCMLVMGLL LEDAAFAAAA SYSGVFEALI AAGTCP ELG SAPALVANYA LSTDVLDHEL RLLVWAALVQ GCLRPEVLEQ AVASGLVRVL LLYVSPGEPH PAVRRWNSDQ LATLRSG AL SRLHTLSPLC PEEYERAGGP GTLLAFAGSS PGATHLEAAL RHLHRLFSMV PETRDALGSA GIIPVLLAVV ADTVGSGG G GGTMGHLAGE RAGNGHGALT GVGSLQPEPV RHFALLCLTA LCSVHAENQR RLRKAGGVGV LLGALEKLRG LDPLLPAPY AVAVLDCIWA AVVPDRKSAA KLLVDGGMEA LLGHLEQGNK SHRPIVLRVL SDLLENPRSH PFFHDWQSDL NKQTAAHLLL SVWMEEDSL RGITGPDGML ANTARPLAGL GKRTKWLPPE NVAYGNMSPA KKETLSIMLD ALPSEIVLAK IYGIFKVLGF D ACPYLDPA DHAVLAAVEK YAKFRQGEVW RAIQAQFAES GVKPTAPDRA RLESGIEMSE SLAAAVREAQ ARLLGRAQEG QR AAEARLF EGMRQQARLE AEYRTIALQE KTPLTLAEMR RAKEQKSSML KNSLQSFQFQ HGEDD

Macromolecule #28: FAP54

• Macromolecule: Name: FAP54 / type: protein_or_peptide / ID: 28 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 332.696375 KDa

Sequence

String:

MASEVIALCH SFEQELAKSL NVLPPVSASK PDAHDAHLNH HRLSQRIAES VSYYAGRLPA YASVPRILVF GDKLFRAEQY QLALQACYK HIRGLELHSS RENLPRMDAQ ARLSSHVQAC FGCAACEAAL LLASDGSVKH PDTLQWLVSC LAQLRAAMSL A LPDERLYW LVLNGTVHVY GIAKAMITAG FAEQALPALV FCIKALEGHV AFAAPKYLPW RTQLYTWAVY GLADCGAVEQ AR ALLADGL KRLEMLVALQ KLDPVPAAPA VQAAFAAARG ALVGLQLRVE MAAGAAVTPV LAQLSAGAAG AGAVGAAGPT ARA GLAALV EALHVPHRRV VRTEAVSGGP LKELFDAAMA VAAPLIGDLK KATEAAAAAD AAAAAAAVAA DAASAEAETA GGAD AAAPA DTGAQQQASA AAAAADEAAV AASAALGAAQ EALPCALHKG LLCAAYNLEQ WKQFEELASL ATSRSDVVYA DAVPT GTGS TTTTAAAAAD VDQTAAQLGT AASILTALRQ LSTAPGVESL RTLAAMLQQA LSRGITVSGT AGAAPPPPPR RVSMTG MLA GGSASPPNGS SGAGGAAATL TATAAGITQT AGSPSHNGGA AGATASAGGG AAGAPPRPQQ SSLAPWQQLR DLIADAS LA LYGSARPLID GVFCADDKEA GLVAELLAAC HSAWAAVELD DGELRVAAAL KLALLLEEEG RLLAAREVLM QAKSVVEQ S RVELMVANRR APDEHLRWVT ASRSQPSDDT AALVQGMTAS EQELACLQVD VLALLARVEL GLGVSEQQGR ATARRTAVM EEQAKRTAQS SIFGQRNAAE LARDEARLVA AGATPPNPQM KERELLTACA KNPYERAVVL MQMTSFQGAD AGRKTQLLQE AGDLLVKAQ AAEDGLFAAQ QPDLRARRDV PPQPKLLQRS PTSVTLVAHP PGPGLKQPPG ARKPPSRYVA YCKSFGAGVG L SINKTATE YPGSGVSVPL GQPVTIQGLR TNDTYLFAVA LYDDDGNIVG GLGTSTPEVL VALPLPLLMC WTHLLAAAVR CG AAGAAAA KRAASVLLPH FVVTTPDVPL WRSNPMDAQR LHRAHVAAAA RPLLRALVQA IYLYGGAALL RNGGGGGGGG GGS TAPGAP VPQVALPCTL PQLRAPFLEE QVARLKSARL LVLGMEVAAL LPDEPLMQEG ALRTYGLLAP LLALRAPRSH LLHK ALAAC HAVLASLTNL VQDSLYRQEH QRSLAARVAA AVTYQLLRLS DEAGEVGAAA HFGRLQLELL KAYDPRFALA GRPAL LPGA ELQEEASQLH DVLLQHPKLS EWAPEPLVER QKDASDLVAR VLPVLGTPAP MDTWSQALGF ETAAEHPRWV ELVVRM VEA AVRKGNPGNA TVVAEQLTWW VRARLRRPPP PPLDAYEAEA AEAAAAGGTA PPWPAKWKLE EAAAALDAAT VAASFEP PP VPSTEGMSPE EASAALARHA AVAAAAEATA RQRLAAVLLL QKRMPALLAR KRLIEKMREN RKRWSPWMAR LNLVLGLQ A AADARRYAAN APARARAAAA AAEAAAAAAD APGPETSGVA GSRPGTAAVN MPPPVPDPSA AGPPPLTPPE GITPPLQAM MHFARAAHLA ARGGAWVEVG NAARHAWNLA RATLSADPAL TAPLPPVRWE RGDAPQPAVA PESILVPAGA GDAGGKKGSK KGGKDDKKK DDKAPAKTPS SPKGGARSSA RSKKGFPVEE APPPPRTYPV VVGARPNMQR AARSLADAVL ELVSALRDGL Q VYTWVAPV NPRHRFDGPP PRRAALAASA STAGAGLGEE ERPVTARVTD SEFSYGSDLQ ADIWFKEGPL DLAWVSRFLG LA AVVLSRG ERWHALVEAG RQWARLSEGA FNERIMPWLL QAAPKAGVDP APFQSAMDAL IRDKNQALDQ LDKVRTLVRE RLG DTPLMA QSMGHKVRKR KTRAAILAAG PGSGAGGGGG SGLPDSDRAS LVSAAYTYRT ASTYKTKASQ PDFLRIPGEY EKVI EVLKR RNEKGAMLLA LHELGDVHAH FGNWGGAATS WNDTLDTLLG PYQALRNWRG RLDGMSPAGT LQAYGLHGLL LGCVL AGKL ARYVHHDHLH LRLEAHRLTA RLAFCAFSAH LGHPQRRAGF ATYTPRELWA AGTDAWLVWS DPYRCPVVDL AGALEG AAA ALLDAGLALE ALPVLALWEH VTRHVLRNLH GTVLCRLLRV RALVALGLLA EAVEVTGGLM GAAALPDPTL DSDYVLK DT SGAVVEPVPA PPYDNSKLPG EPGNKAALTH IADTPLAPAV EKLYGSWLVA HLALARAQVL MLAGSVPNQW RGVDWRTG E RTAAPKPASP AKGAAKGAAA AAPEPPGAGL PEPVEPVMLE RAHVLLRKAL AMASGEDQPP SEEPSSAGKP AAAGKPAPA RAPSPGRAKS PTGKGKGKGG AGDGAGSAAA APPEVPGPPP PPPPSASQRA EVNVRALLLM SELEQLRWMP SRGLAHALEA ARFLGEHAD HVNTPQQTDN DELERYTLAP ALWFLARGAA VRCAAALGHA AHVHELVAAA TSAHAPSEPG SAKVPSGLPE L LHCTGMAH VAALTLAAEG RTSDALAALA AVAARYRSLC VYDGRLAAVL LDAAALRDRL GLREDAAELT AQGLAVAEGY CL ELGLGEA LEAPELTNVY LDGTALYAHA LGAAAVHASR RQQHAEAERC AARAVLLLRS HTRALPATHA AALLLLGRTC RMV ALCGDG VPVDGQPTLA SGAPPASTAT LTSAGAGAPG DPAATAVAAG TARAAAGGFN ASAGALARTA TAGRGGGSAG SSGG GVAAT AAKLSAARSA LCASITLAAV DGGHLRSLMR GALLELGSIF IAGLDARSAA ACLRAGHAAA AKADLVALSS HTLAP VAAA QLPDWALAHV RGQEALFGKK SSNGAISGAA GAAGSARPVA TSSSGARPPG TPPGGKPGAG GGSGDGLSDA DAARMV FCL LGGLLKGLEA LPVGGGARAR GEAQVAALHA ALRAACAKYG TDACFAEPPL PPSPPDAVPP PPEGSVIVQW HCQDGCW QE ARSWRAEGSS GAPDGPLSDS ALLALQPVPA YASLLFVVAA PSHDGSPGPH CGEVTFAVKD VRELQRRAKA LRARVEAP K AATDILGYAA PSQVELGELL RAAERLLSAV PRNSEDGSSS AGFSAADSGL GGFGSSELME GEVRPELDVA FLCKLEALL ALEAGLDVND KTLGSWLVQT LPVMM

Macromolecule #29: FAP46

• Macromolecule: Name: FAP46 / type: protein_or_peptide / ID: 29 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 289.610406 KDa

Sequence

String:

MAQPETLHQV LAQPADASRV KQLQTVHEAI TASLSGQQPG ARVGLETLVL CAEAALKVGS LDVAHDCLER YFLEQRRYTV GMAPVELRD QYLCRAHFAR GLLVSERSKG LKGKALIDGT LEGVKHVMAG LEVAAANQPR YQFLVYNASV HHWRVVAPLH R DGLRHHLL ASTDRVVQAL DKVPGHEEWK VRIYTALALC QSDAATVAAA QAEAAGGKAP AKGGGGAAAT HDDAAKTLQR AY DTAAAAK LTELQKDVAR LQVHLATLAA AAGGKGAKAG GGGAAGGAGK PGAKGAAAAL DEEGALRLIQ AVLTGRPDRA VAE QQLREA VAKVDPKGPD GPVKGALLRP VSRAAWAAAL AGLPELAERW ASRAAASSDS GPRTWSDMTR VQLALQALGP AGDT SLAPV VVAAHVKALE QLEDVLTTFT KLADVEGIHA AARLAWNAGL LLLQPGLRKH VKRAFNAAAR ALAVAASPLT RLRAA LHLE AGKCDAAEEA LIKAGQEVGK ALALDYLPPP AEAAAVPWLA RPLDRWAAPL ARALALRTSA EPANPEEEAV SLIERS KES RNPAIKSDLL ARAREKLRDL PHPPPPAPTD PPGPDRDAAH AAARRRTAVW AELMRSAASS RMDEHVLAAA PHALCVS WD PAVDREMVLL QAQLAHYEAE AAISALRRRR ADISPPTRPS PPEVDGEGVR QPPATTEQLQ ELVVQASVRS MRGAMSVN E PWLTLNNAVQ LYNAALPLMQ QHRYADLYRW LRPVAEALVA LPVADSDGPL AVAVAEALGR AAEHRLLLAT LRAKAARDA GQELEDDDDE DSLDEDGNPP PAGDAGPHFN RRSPAYKPLP DARVAASGLD PRGFPALARL LASIGTVTAV AEAALERAGG ASTQGLLEM YARLQQYRGV SSGLPAGAAA ASAATSRVVS AIEALSSANR VNEEKQPAGA GAEKGGGDKG RKPHGSLAAD A AAAIALLR ALPGGPPLEL WAKMARAVAD AGVWPAALEC SAAALAALPG AGRDLDVLRL EAPSDVPEMT PAGWFWASVA LS VRAAALL TLVDLPSQGA ATALTVRREA LLHAAQAARC AAFVNKADLC ESACRVAWNA ALPFTTKPLL RAALIRPLGT AVE ALNRVG PADKGFQVRM NCLYVESLAA AKRWGDAVAA CDAASRAVKS RSLHRPLMGW KAACLAMLGK NVNAEMTKVK EHPP EAQAY AWSVLSHHSA ARYDQIAAHK AAGEAVEHHG WLKAVALAGY AEWLLSSSDD KEAAEDALLA AADALLEFDT GDLDG DGTD DEDDATKAKP RSRSGGGSSS GRAGGGFRRV LSRSYSRGGG GSADGARPSE GGEGAGPAAP DPNRVPEELG STHLER LAR LYVMACQAAP NATDHTDYML AAHHNFMRLL TQALHSAAHT AFVAARDSYN GEVAAAALEG RDPPPETPLP KPYAVVP DT LIGWGTWQIT PELLETLASD AATGAVTALS RELLPQAELT LAYLELLQAC LRARGYHCHC LGLAQLQRVL ARMVLRDE G MYVATSLGLV AALDELGLSR EAGEVEAALG DVACIGPQEE AQAAEQAALA DLVLAAAGKL EAARPRSALA VGRLSFLAG GVRRANNAAN AAAAAPLPPV AAASQAAAAA DAAAAASFTA AGGGRGGRES PSPHDDGIHY IGGPAPGDSH GQLPEWMEDA ARELGNVDL AGSAGGLLSH VSGQLLLRPF TLHDVWLKKG DYLLRRGHYA AARQLLSRAR AHAADCGNRE AEARCLLALS R TELAAHNP VEAVALVQAA QRFGGDIDFW AELLVQYVDC RLAGAHSTTS DAREALQGGI AMFLALARDD RAAEKPASAA GA VLRVRLA RLLLTDMEML RGQGVSTWRK SYDGAVTLVN QAIMALAARE AGLPHIEALL VQAELMLAEP THIKDLRPRL KKV EKVLLA AEEMAVRFHA EATPRDLAPR CVTPTARLLA CVRCRLAEVQ LAAAEERERL AGADREKARP KFPHMRGKRD VQVV IDFID EAGGAPPAPP GLLPEDSALA LATGAAALVA AAPRDRARAL LIAGRCLAFK FMMAAPEALD PLRPFVPPPK PPGAP KRPP AGAEEEEDEE GPDTAAADAA AEAAEAAATP EGAAALRLQA QAAAMLSSAL ASATSVQDWA LGEQCALALS TLYGQL SPG LACRSLAAAQ ACRAAAGGQA LLRAAAPAQQ PEVLALAQRD KLSEVLPAPQ DNVHYSALRR MLSGLKGAAA RLDAAAA PP VEQQLAALPQ DLRVLMLYLS PDGGRLYAAA LNIPDATAEP TPPLNAEKSK KKTDASAPAA AGPRLSLLHV VEVDPAAV E ALVAECRAYR RGVERTLREA IASAAAKGVG VPPEDDRPDS PSKKGKKPGS ATKRPGSKQG PKSGPGAAAA AAAAAAAAG EAGKVQVFDS RLNEEWSGIL EQFEEWLAPL APWLEAAVPA LPLPPPGSPD GKKEKKDKKE AAGPTKHKVA LLLDPALQSL PWEAARHLA TTCSEVSRSP SLQALAACHT LPRADSAAAS EHAAAAAAAP AALPPLDLGR LTVIVDPRHE CSTAQQARGP Y TAQLIPAL SAPELTQVLP AASWWGPGGP GGGLEGVPGR APSPDTYASL LAGQATGGPC TGLLFLGVGR FAAHVPPAVL AS APLGGCE AALLFDRCNT DDAYWAQLYR DNRKSAEQRR LESPGRVATL LLAKGVRTVL VMSAAAPPAA VVKLMHGVMA GLA AGRVLG EVVYSLLTGG GGSGGAGAGV LDEFELAHLR ACLQVWGAPG LLGAVLTQGN KAAKGAKK

Macromolecule #30: FAP275

• Macromolecule: Name: FAP275 / type: protein_or_peptide / ID: 30 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 18.171365 KDa

Sequence

String:

MAAKGKQQWD FLKADANTPA SPAHYYEPLN AKKEGEFKPG WNTKRRGPAW EAERQAAIMT KEQKNIGCVA LRSERLNNAQ QQSGFNPIA HTERAADGSW VPATNAWMHQ KVGVKQQDPR AAAADTLKHQ AEGASRAAAI AEMRKERIAA GGASRPAAGG G VKDALTWG

Macromolecule #31: Unknown protein

• Macromolecule: Name: Unknown protein / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 3.252 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #32: FAP47

• Macromolecule: Name: FAP47 / type: protein_or_peptide / ID: 32 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 319.999812 KDa

Sequence

String:

MAQLQVTPAQ LEFANVQPGQ VLSAWVTVKN LDARARTVKV RPPKTKYFAL REFFKPHSLA GGLETRFEVF FDGDKFEADR QYAQDKIVI QTDSGSYDLQ IKASTPKADV RIVGDLNMGL LPAESKATKR FQLVNQGAAT AAFKIDYDRA LPISITPTAG A LEPGGEGQ DVAVTITVGE PGSVAGEVTV AVAGEPPKKV MLTATVVRQS FDLVDSTGAV TSELDFGSLY YGQAMEREVT LF NNGPVEA RYVISYGTLA EMRSKVDDDA AANADPTDPY ADVIMAARQR QKARSTGDNP FHVTPLSGVV PPYSKASVSV RYT PTQPAA VKGFNSLMPT SDQAMRTYEY FAVVELLGMP GKLKLPIRAR GMPGGLRMSP PNMFFGDVPT YAWADQLLTI TNTN TLMPA RYQMVKSTPY FAVEPSSGTV LPGGNVQVLV RYQPKAMGKH KGAIPIKVMS EAGQLIQEMT LELGGSSLRV GDKVA PVGG TDKLPEDFTK PRHFVDDEQA MLSTLQAHQK TNRLLAKPWE KPDLADTFAD ASNKNTLSRG EAEAMAAHRD KYTAAM RTQ RTAREYQKKN GRVAEDDVNL GMDTRSGLAA PEPKLSRTVD PLWRVDSTKE AAPRMLRQLG PADLEGTHQW KARPQSE QE RIDVTRELTK EELSRLVVGP RVVDFGKVSM GSTNTRHLVV HNTLDTSLNV VLDLKPLEML RESPSISQVI PPGARGAF P LVLQASEIRS VNEKIEYVVN GNHVLSFPLS ADIVPVNLDF SAEDLNFQFS LDNWDEAVEK LLILDNPHKF PVSFTVEST NPTFQISSGG GGVIRSQSSV EVVVRWAPDM LGPPKQEGHL IIKMVGGEAP KRIYMSGDLP EGNVRPKDKE LVAPVAPVGT QQSVMMQIK NHGSRDTAFR FHANDVLTIK PSGGRIGTEE TLDIEVFFIA HDPGVLASTL VLEMRGGNVV RIPFRAEAVV P HVEVLQDE FVFNQVFVGS SSRLPLTLHN TSPVSATLMC DLVQHPEFQL LCPKDAWSTD EYEACPLEAI GATGQQVLGS RF GSARGSR QGSRRQSSSL GFRGMSNEGY KYKITINPGK ELPLLLNFRP AEQKHSDVEL AFTLVTAGGY NVPPVRRVVT GSG ETPRVI LSKTLVDFGT KIVIRSNQIK APYSLDLYIT NQEEEPVTVA FGEPVVAPHA AACPVKGIFS MDLAPLAPLQ VGGQ EMTTF QLRFCPRDAR TYEALIPVYL DGDMDEPYLN IEVQGVGQYP RLAFDVRECL LPPVPLGLRS TTTFYVLNNG YDNLE LKYK LPADEGHLPM EVEFPEGTII GLAKDRLPVV VSFMSPKPMS FTANIDFMDE DGKRFSMPVT GTTDNSLMTH HAFHQL NKS HLVFSHEVGM PVFLEEAAYE LPPPSMCLGP SWPCRGVARY LNATTTRGPF DDLAKQMLGS RGKLLVEVVE MLSGKNL PG KVFKLSNNRK EAVKQLLGQY DAVLVFLKSH GCLLNAVKPE MLLEFDDFCR VMNERSTAAT DNHDELEALE MWAEVESN F DLVSGQAWNA TIMQIFKVFV LGRVTLKSLR ALPGLEGCEL PPDSSIIGSN YYSVSESSLL AWMSAHCLRE FGPKFPRIT NFDSDLSDAS VLFALLSGYW PGLDKKKKML RLDCRDEQDK RENAEMVLKI MTDLGLPYEI TVDDIVLPNP QDMMVFVLYL HHTLPQFVP RAAVDFACKL GETQCKELEL TNPSKKAISY SARLEGHRDF SIESSSIRIE PKASAKLLIR CTPTNTLSQA A RLVLMSKR EGGVHAATLV FALQCKVNTR APLKRVRTEG PLYDMQQFEF TVTNPFPADG DFTIQILHEP AEPLPKKEED PA ATKNAKG NKSTNRRTGR KSTGLDATLG HEDPASKLGR VWPDAFGLDR NRLRLKQNAS ERIKGFFLPF SMTTAYCTVL FRD KDQGEF CYELVGEPGL PAPIMEVKGN VPLEAPQQVY DLMLPFPNSA LEGAKRAFTE KHPLAKDKEQ VALLKQDPFK GMEV MYEVT QTNSLMSSPG HIVLSARPSM GAAAADGALP GGGPVSPPPG NATGGGLGST QRPTTRNPNA LPPPEPNTLR MHLNP IGTG IYPTRIMLTS PLDVRVVDLE LTAQTMTQSF VLEFNTSARQ AITQEIPLVN TGDAQMTVTA TINGSAWSGP REVSVS AGA TVGYPLQFKP LMPGDVKGSL ELAINATGER NTYTLVGRAA EPTAEGQIII ECQARKPAVK QFNVPNIVGT GTEYKVL CD LDFMVGVPSI KVPPGMASQY KITACPQRSG TYNGTITFTT ADGQFVWYTL EVRASEPPEV GTIDVRATVR TAAAVTVG V VNPLRKDMEL AVRYSEVALC GPPSISLPGS QEPVPFEFFY APLVPGTSTG SVVLVNDDVG EFRYLINMTA DPAPPGDLP AMTAELGKSV SQPVVVENPL DTPVVLAASS SNPRNFAITP AMVTLPAYGR VELAVEYTPS TLDHEEEATI TVDGDVVGCW EYNVRGRGE LPTIMEAISV SAMLGGQGQG VVQWRNPFPE PASVSVQLRT DEPAGVFELL RISGGPQLPG AGASSSHSIG S GLGPTTSH TSLGGGPSAA SLNAGGRQAA ARSGSSVATI QETTVQPYGT LLVPLRYTPA ALKTCHCEVL VAVEGSSYSL NT LEWRFPV RALTEASLSG VVFKYKCKAR ARIEETMEVV LNGLQKVGPE DEFTHEVVLP PEHRGFLEQA LRVEPVVAAP VRL ARPDAP LRYRVCFAPQ RMLPPTSLEL VINKSTGGRW RFEMQLQATE PDLDGVLTIE AAVGATSYIP LRLYASGSEP QPFT ASFPN DTPMVFHVIP AKGLLPPAPK PGEPEPEPPL QVSYSCREFG KVVRGRLYVQ TSDMQFSYEL RGRMPTYVPP TPAQF APSV DNKLSAELGA KLSLGRPTGK NFVAANLRAA KAGLAAAGAG GAAGGPEKEP ANRRR

Macromolecule #33: FAP279

• Macromolecule: Name: FAP279 / type: protein_or_peptide / ID: 33 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 43.267051 KDa

Sequence

String:

MASVVSEPHK ELVGGGENAI YIKECTELHL GSKGIEKLRG FEVFVNLESL WLNGNKLKKL NNLDAQTRLK ALYAQDNQIC TLKGSLEHF KFLETLDLSN NQLRDLDKQI KVLEKFKFIK ELNLKGNPLC EEPDYRLIVI HRMPALKVLD QHVITALERR K AEGLIGGD VATLTVAFGK RLPPYDPAWD EKVPERSALE QQMSKEAATI RDTLRHEAYM KERGMFLHDP HPQAPRGSSL PP NAGTVRA MQIWRQQLEA TGQHVSQSGS APASPAYGGA DRSSPAGTTG RGGGVGSSAQ GGRLRHTSGS GRGASLSPSP NRT GGLAGT GAGAGGEGPY TSKDKLVLYT LRSGSDPLAT GPAAATLTRP PPGTIKFEAE EYQQFLTTRA AGAGGWQVGK TMVP L

Macromolecule #34: FAP266

• Macromolecule: Name: FAP266 / type: protein_or_peptide / ID: 34 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 81.663047 KDa

Sequence

String:

MEAELEAVVS TSYEGSVDVQ GRWDGHGRVS FKSGATYDGT WRAGRMHGHG KFVFPDGISY EGEFQDNQLT GTGVYTWPSG AQYEGTVVR GRREGRGRLS FTKSAAVYEG DWRDGLRHGQ GVLYTNVQRS SYYRGQWLDD TKHGAGVMRY DNGDVYDGQW E ADKKRGLG TMRWESSRQQ YAGEWDNNVP NGAGLHVWFS EQEAGIPTHA QLLMHNRYYG HFKNGRRHGY GVLYYATGAR YE GYWQGDL KHGQGCYVFE NGEVWAGAFA HDRPGLAPGE AFAPASTGVQ LQIDDLVEEE ENPTASRRAI SNLLLCYNTE LRM LYDKYC KRPSHQLPPK MPRLSFSVLA AQVWELANDC RLLSPALTLS GLDAICATSR LMPERLRQFR SQETPKAMDY LTPE QVRFW RHLATEHAPG GGHSPVADLT FRAFAEVLVR AAAARFAHLP SLERRLHQAL TLHVLHLVNK AKATLPSPPS PTPVL PPNI AVEAAVAALD AVRPRMAEIF LAAAGAEPPL SKEQQQQEAA APEARGGATA AAGGPTDPVY GVTAPARALA AALEAA AAA GGRLAYPLGF KGALLQLLWN YVSRNAYGPV QRMTMEPVGS GDGSEGAGFR TAAESLAAED EQELMFVMDQ PLSWAEF VD GVWRIATDWA ADVSDAASHA VVPLSDRLGA VLGLEPEPEP EPPAVGGGGH GRAKSAKGGA GGAVLAKGLS RKGLGVRG A GVGEGGEDGG GGEGYGGGSA LQSLESGERP DMAAGGADAA

Macromolecule #35: FAP76

• Macromolecule: Name: FAP76 / type: protein_or_peptide / ID: 35 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 172.707297 KDa

Sequence

String:

MDWEKKTVAR ASLREAAADV FNDTKTPGQA LLSALETAET IRKDRYGSTV AAKMKAAREY LPALEVRHPP RSDTTIFGKP GAEVASLLP DGRPRPGGKV TLDNVKSMME EQMERHKQAL DSFVSTRDDA RAELEAAVAA AAADFQSQLA ADDAGIAEDM G ALDDDKVM RLTEQEVHQV WDCVAQRIPQ RARWIEECAA ALEAAEEHRR ETVEAALVSL CADLNEAAVV SEGEVERLVE KE AMALNVA ILENRRAYAD VVHRLQVAEV EAERARRAAW TEGYRKWRTL RTQHAIKVFV DRIRSPEFAE PADRLAAFGN LRD RQAGVL SGLAAHWARA GAVTPPHLSA KKVAGWTADA TALDAAWARE RQQMLASLRA AEDALDSRAA ALLAALREEV GEYQ GYRPE ELDALVAGSA GEAVAARRAA ALEVLAAAEA FLDEQAAGWA AVSVALSAWL NRLCAMFDEH RKLTGADESE VRGGL KAAR EAFNAADAER EAALDAAVAA VTRGSGEAAL DARVAEALKR LDDIEEGYRG FHRDMTALAR AYPARVRGAN DTYHVS LCD QLGCKAQTGA AGWPALNGGG KKQVLGNIKL PDGRSFALVT DMLQHLLHPE DEEKKAKEAQ AAEEAAAAAA AAAAAAA AA PPAPASGRAA TPPKSAKKGA AAPPPEPPPP PPPPPEPEPT PTPPPAESPL SNAGTPLCRS IALPEDILRR TIESIQSG L LSDMMTFCQR SLEAAEDWSA EREVALTEEL DSWLRHHRPR AGRIEEEVRM ARSVELVAQR RRVELHLKAQ STAVKAQAA AFEEGLRALE SEARKGVSRL RATEGFLGQC LSSKAIAIRQ REAEALRVRL DKELAARVAE LGEKTHAAIA RITDANRKFE SDELKTFES GGKYADENAA AYKRSLAAID AHLERAWSEQ RGRLEASRAA LAGEADSSLS EVVGLVPSHL EDMGLLEKMD S LVEGARRK AAGELDRNKA AAAAITAQLD QLDALLDGRK AAAAPPRRRR VSGSGPAPGA DGRRPDSPGA ASVASSARPG SV GGGPPGR SPGSSASMAG PAGMRSSPSA ASMQRGSPGT ANGGGGGSPP GRRGDGSGGK PAPAEVPAAP LERCRAILEC ADS LRKALL RQAKVLDFLQ SSGLVAPPVD LRVDAAGVDP PPPPLGSPEA LATMGGGAAG GSPGGRPGST LAGAKRPTTP SNGK PGAAA GAGAAEAPPV KLATDVEAVI DACKKETEAV AKTYYAAKDK ARPIRYPARI PAAVEELLRG TDAVLEGLRQ QTLSY VTEA VKELRTQVIR SYRSLERLPA VALALLCEHE VGLLAAAHAG LVGELEGRRG ALLKLVEVHR GSLHPGMMMP ARRPEL AAV KSSESDRAAA HAALLSTYGG QVLDMARRQA PALQGRLLRL SSQLAALLDT FLMPQDLPPP GESDSWVYDL PHKDTKQ LL RLALAQAEAE VEAPPPVDAK GGKPGAAAGA KPAAGAAGAK AGAAAGGKGK DAAPVPDRPY SPGEWALPAG NFDLVSLG W LDEEGKPPHG LPQPQQVEAA APTKASKPAP AGKRPASAKP EGAPGRTPLV PVPAPLVALD TPCHRCVVRA YRSAVQRLE ESLRSAMSGH RLRLSSWAKD EELWGRTWAQ LVGRLEGL

Macromolecule #36: Unknown protein

• Macromolecule: Name: Unknown protein / type: protein_or_peptide / ID: 36 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 5.805147 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)

Macromolecule #37: Unknown protein

• Macromolecule: Name: Unknown protein / type: protein_or_peptide / ID: 37 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 12.698645 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #38: Unknown protein

• Macromolecule: Name: Unknown protein / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 4.358364 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

Macromolecule #39: FAP246

• Macromolecule: Name: FAP246 / type: protein_or_peptide / ID: 39 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 120.452891 KDa

Sequence

String:

MPSDGASVAG SSRSSMSRAS RASKRGAKKS LPPPPPPEPV EEPKDAFQPS MLSAGLSQLG RTADGLKPAY LTLSLTGTDL ANADVLGAF PHLQTLVLRD NRLVELRGLA ALRHLTAVDV SGNKLTQVLD LRLPADGASG PTNLRSADFS RNALDMLRDL S PFSRLTSL SAAHNRLERV GEGLTSLTLL KVLDLSHNRL VSVRGLERCA NLRELRLGHN ALQSLEPLAG LSQLQVLDVS HN RLAQLSG AAGLSSLRTL DVSCNRLGRL EELAVVRGAS LLGTLDVRGN PLDKAMCLRL HVVHLLPQVV MLDGVAVESK EKV LAANMH GADADSLRLI RRKYFPNGEL DDGGGAIPPL AAGLVASAAE EEAAADGPGP GDGAYVRLDA WAASIKPEWV LGGG GGNVV SLADQLASVC APPRPTTTAA AATANGSTHN STSGAAPGAG AANGHHGVRG AGVGAAPSGA AAGGKPSAAN EAAWR RAQL ERARVCWRWV VTHVAATAKL ETTWDVAAPL FGHTAQAAAI ERVLLGNGEP APPAAPPPPP QLLGAPLAPP AAAPLP GGG TWSERVARLF TLLCRACELE AVPIPGYWKH AALPPGSRVH AHNHCWAGVK VNGRWRLVDP TAAALAGGHF PFFVPPD AF IYSYWPLEAA WQLLQEPLSQ EVWWQLPYAS VAFFAEGCRL GDASLAAVNT LLPIRQGGVL PAFALAVAAP RRRGCHLA Q RLYDRARRVV AEWPPREPGA PAYAFQQVVA GPHGQVEVSF GADSHAAQLH QMYFSLPAPG EYEMEVSHVR ELPGGGLTL AVEGLPAPGL ALRVVEEQQL LRVKVTLPGI QPHDEAEDGI LHSPAVLRTP LPFGSPHWYE AGCQLISPPP HRPLEADRSE PFKLVVPGA SRVGLMAEGL QQPIELAAAD DDSFAFSTSL QVPRCRAATL MAYMHNDHLS SCAWVPLVQL AVLPQSQHMV Y TPIAVEVP EVGDDDPAAH FAREIFKAMD KNLDGGVCRR EILAAFRRNR QHADILKCPA RIRQDDGTFE RFVDVFMQID TS KLGTFTF NELAFYMGVL PTGYYQYSDD EDEDEDFDSG DEQDLDPEER AGRQAELDAE LDAPDDEGEG GGGGPGGGEA SGY TGDSAG GGGGEGEGPS EA

Macromolecule #40: Phosphopyruvate hydratase

• Macromolecule: Name: Phosphopyruvate hydratase / type: protein_or_peptide / ID: 40 / Number of copies: 8 / Enantiomer: LEVO / EC number: phosphopyruvate hydratase
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 51.664762 KDa

Sequence

String:

MSVQEYIEKH GLEKKVEEVL NLCVKEKPEE PLSFMAKALG QLTPPEIVKV VGRQIIDSRG NPTVEADVYT RKGMFRAAVP SGASTGVHE AVELRDGDKS KYLGKGVTKA VENINAIIAP ALKGMDPVKQ AEIDQKMKDL DGTDNKGKLG ANAILAVSMA V CKAGAAEK GVPLYKHIAD LAGNSKLILP VPSFNIINGG SHAGNALAMQ EFMILPVGAS SFSEAMRMGC EVYHALKGLI KA KYGQDAC NVGDEGGFAP NIGSNDEGLN LVNEAIEKAG YTGKVKIGMD VASSEFYTED GMYDLDFKNQ PNDGSQKKTK EQM LELYNE FCKKYPVISI EDPFEQDDWE PCAKLTTENI CQVVGDDILV TNPVRVKKAI DAKAVNALLL KVNQIGTITE SIEA VRMAK EAGWGVMTSH RSGETEDSFI ADLAVGLASG QIKTGAPCRS ERNAKYNQLL RIEEELGENA VYAGESWRHI GW

Macromolecule #41: Heat shock protein 70A

• Macromolecule: Name: Heat shock protein 70A / type: protein_or_peptide / ID: 41 / Number of copies: 4 / Enantiomer: LEVO / EC number: ec: 3.6.1.3
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Molecular weight: Theoretical: 71.303758 KDa

Sequence

String:

MGKEAPAIGI DLGTTYSCVG VWQNDRVEII ANDQGNRTTP SYVAFTDTER LIGDAAKNQV AMNPRHTVFD AKRLIGRKFS DPIVQADIK LWPFQVRAGA HDVPEIVVSY KNEEKVFKAE EISSMVLIKM KETAQAFLGA DREVKKAVVT VPAYFNDSQR Q ATKDAGMI AGLEVLRIIN EPTAAAIAYG LDKKDSGLGE RNVLIFDLGG GTFDVSLLTI EEGIFEVKAT AGDTHLGGED FD ERLVNHF ANEFQRKYKK DLKTSPRALR RLRTACERAK RTLSSAAQTT IELDSLFEGV DFATSITRAR FEELCMDLFR KCM DPVEKC LRDAKMDKMT VHDVVLVGGS TRIPKVQQLL QDFFNGKELN KSINPDEAVA YGAAVQAAIL TGEGGEKVQD LLLL DVTPL SLGLETAGGV MTVLIPRNTT IPTKKEQVFS TYSDNQPGVL IQVYEGERAR TKDNNLLGKF ELTGIPPAPR GVPQI NVIF DIDANGILNV SAEDKTTGNK NKITITNDKG RLSKDEIERM VQEAEKYKAD DEQLKKKVEA KNSLENYAYN MRNTIR EDK VASQLSASDK ESMEKALTAA MDWLEANQMA EVEEFEHHLK ELEGVCNPII TRLYQGGAGA GGMPGGAPGA GAAPSGG SG AGPKIEEVD

Macromolecule #42: ADENOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 42 / Number of copies: 4 / Formula: ADP
• Molecular weight: Theoretical: 427.201 Da

Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

Macromolecule #43: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER

• Macromolecule: Name: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / type: ligand / ID: 43 / Number of copies: 8 / Formula: ANP
• Molecular weight: Theoretical: 506.196 Da

Chemical component information

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

Macromolecule #44: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 44 / Number of copies: 97 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

Macromolecule #45: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 45 / Number of copies: 97 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #46: GUANOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 46 / Number of copies: 96 / Formula: GDP
• Molecular weight: Theoretical: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: filament

Sample preparation

• Buffer: pH: 7.4
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.5 µm
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Image recording: Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 39.6 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

Startup model

Type of model: EMDB MAP
EMDB ID:

9385

• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 80007