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- EMDB-21107: RSC core -

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Basic information

Entry
Database: EMDB / ID: EMD-21107
TitleRSC core
Map dataRSC core structure
Sample
  • Complex: Remodeling the Structure of Chromatin (RSC)
    • Protein or peptide: x 18 types
  • Ligand: x 1 types
KeywordsChromatin remodeler / RSC / GENE REGULATION
Function / homology
Function and homology information


regulation of sporulation resulting in formation of a cellular spore / chromatin remodeling at centromere / positive regulation of pseudohyphal growth by positive regulation of transcription from RNA polymerase II promoter / regulation of nuclear cell cycle DNA replication / plasmid maintenance / DNA translocase activity / nucleosome disassembly / RSC-type complex / UV-damage excision repair / SWI/SNF complex ...regulation of sporulation resulting in formation of a cellular spore / chromatin remodeling at centromere / positive regulation of pseudohyphal growth by positive regulation of transcription from RNA polymerase II promoter / regulation of nuclear cell cycle DNA replication / plasmid maintenance / DNA translocase activity / nucleosome disassembly / RSC-type complex / UV-damage excision repair / SWI/SNF complex / ATP-dependent chromatin remodeler activity / sister chromatid cohesion / nuclear chromosome / sporulation resulting in formation of a cellular spore / rRNA transcription / chromosome, centromeric region / ATP-dependent activity, acting on DNA / cytoskeleton organization / meiotic cell cycle / chromosome segregation / helicase activity / transcription elongation by RNA polymerase II / positive regulation of transcription elongation by RNA polymerase II / transcription coregulator activity / double-strand break repair via homologous recombination / lysine-acetylated histone binding / base-excision repair / chromatin DNA binding / double-strand break repair via nonhomologous end joining / G2/M transition of mitotic cell cycle / double-strand break repair / histone binding / DNA helicase / sequence-specific DNA binding / DNA-binding transcription factor activity, RNA polymerase II-specific / chromatin remodeling / RNA polymerase II cis-regulatory region sequence-specific DNA binding / chromatin binding / chromatin / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / ATP hydrolysis activity / positive regulation of transcription by RNA polymerase II / DNA binding / zinc ion binding / ATP binding / nucleus
Similarity search - Function
Chromatin structure-remodeling complex protein Rsc14 / RSC complex, Rsc14/Ldb7 subunit / Rsc1/Rsc2, bromodomain / : / Rsc8/Ssr1/Ssr2, zinc finger, ZZ-type / Chromatin-remodelling complex, RSC SWI/SNF subunit Rsc7/Swp82 / Chromatin remodelling complex Rsc7/Swp82 subunit / DNA-binding RFX-type winged-helix domain / RFX-type winged-helix DNA-binding domain profile. / Chromatin-remodeling complex component Sfh1/SNF5 ...Chromatin structure-remodeling complex protein Rsc14 / RSC complex, Rsc14/Ldb7 subunit / Rsc1/Rsc2, bromodomain / : / Rsc8/Ssr1/Ssr2, zinc finger, ZZ-type / Chromatin-remodelling complex, RSC SWI/SNF subunit Rsc7/Swp82 / Chromatin remodelling complex Rsc7/Swp82 subunit / DNA-binding RFX-type winged-helix domain / RFX-type winged-helix DNA-binding domain profile. / Chromatin-remodeling complex component Sfh1/SNF5 / SMARCC, C-terminal / SWIRM-associated region 1 / SNF5/SMARCB1/INI1 / SNF5 / SMARCB1 / INI1 / Remodelling complex subunit Rsc/polybromo / Zn(2)-C6 fungal-type DNA-binding domain signature. / Fungal Zn(2)-Cys(6) binuclear cluster domain / Zn(2)-C6 fungal-type DNA-binding domain superfamily / Zn(2)-C6 fungal-type DNA-binding domain profile. / GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain / Zn(2)-C6 fungal-type DNA-binding domain / Snf2, ATP coupling domain / Snf2-ATP coupling, chromatin remodelling complex / Snf2-ATP coupling, chromatin remodelling complex / HSA domain / Helicase/SANT-associated domain / HSA domain profile. / SWIRM domain / SWIRM domain / SWIRM domain profile. / Bromo adjacent homology (BAH) domain superfamily / Bromo adjacent homology domain / Bromo adjacent homology (BAH) domain / BAH domain / BAH domain profile. / SANT domain profile. / SWIB/MDM2 domain superfamily / SANT domain / : / SNF2-like, N-terminal domain superfamily / SNF2, N-terminal / SNF2-related domain / Zinc-binding domain, present in Dystrophin, CREB-binding protein. / Zinc finger, ZZ type / Myb-like DNA-binding domain / Zinc finger, ZZ-type / Zinc finger, ZZ-type superfamily / Zinc finger ZZ-type profile. / SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains / SANT/Myb domain / Helicase conserved C-terminal domain / Homeobox-like domain superfamily / Bromodomain, conserved site / Bromodomain signature. / Bromodomain / Bromodomain profile. / bromo domain / Bromodomain / Bromodomain-like superfamily / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / Winged helix-like DNA-binding domain superfamily / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Chromatin structure-remodeling complex protein RSC6 / Nuclear protein STH1/NPS1 / Chromatin structure-remodeling complex subunit RSC7 / Chromatin structure-remodeling complex protein RSC14 / Chromatin structure-remodeling complex protein RSC30 / Chromatin structure-remodeling complex protein RSC8 / Chromatin structure-remodeling complex subunit RSC4 / Chromatin structure-remodeling complex subunit RSC9 / Chromatin structure-remodeling complex subunit SFH1 / Chromatin structure-remodeling complex subunit RSC2 ...Chromatin structure-remodeling complex protein RSC6 / Nuclear protein STH1/NPS1 / Chromatin structure-remodeling complex subunit RSC7 / Chromatin structure-remodeling complex protein RSC14 / Chromatin structure-remodeling complex protein RSC30 / Chromatin structure-remodeling complex protein RSC8 / Chromatin structure-remodeling complex subunit RSC4 / Chromatin structure-remodeling complex subunit RSC9 / Chromatin structure-remodeling complex subunit SFH1 / Chromatin structure-remodeling complex subunit RSC2 / Chromatin structure-remodeling complex protein RSC3 / Chromatin structure-remodeling complex protein RSC58 / High temperature lethal protein 1
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.07 Å
AuthorsPatel AB / Moore CM
Funding support United States, 3 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01-GM63072 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35-GM127018 United States
National Institutes of Health/National Center for Research Resources (NIH/NCRR)U24GM129547 United States
CitationJournal: Elife / Year: 2019
Title: Architecture of the chromatin remodeler RSC and insights into its nucleosome engagement.
Authors: Avinash B Patel / Camille M Moore / Basil J Greber / Jie Luo / Stefan A Zukin / Jeff Ranish / Eva Nogales /
Abstract: Eukaryotic DNA is packaged into nucleosome arrays, which are repositioned by chromatin remodeling complexes to control DNA accessibility. The RSC (emodeling the tructure of hromatin) complex, a ...Eukaryotic DNA is packaged into nucleosome arrays, which are repositioned by chromatin remodeling complexes to control DNA accessibility. The RSC (emodeling the tructure of hromatin) complex, a member of the SWI/SNF chromatin remodeler family, plays critical roles in genome maintenance, transcription, and DNA repair. Here, we report cryo-electron microscopy (cryo-EM) and crosslinking mass spectrometry (CLMS) studies of yeast RSC complex and show that RSC is composed of a rigid tripartite core and two flexible lobes. The core structure is scaffolded by an asymmetric Rsc8 dimer and built with the evolutionarily conserved subunits Sfh1, Rsc6, Rsc9 and Sth1. The flexible ATPase lobe, composed of helicase subunit Sth1, Arp7, Arp9 and Rtt102, is anchored to this core by the N-terminus of Sth1. Our cryo-EM analysis of RSC bound to a nucleosome core particle shows that in addition to the expected nucleosome-Sth1 interactions, RSC engages histones and nucleosomal DNA through one arm of the core structure, composed of the Rsc8 SWIRM domains, Sfh1 and Npl6. Our findings provide structural insights into the conserved assembly process for all members of the SWI/SNF family of remodelers, and illustrate how RSC selects, engages, and remodels nucleosomes.
History
DepositionDec 11, 2019-
Header (metadata) releaseJan 15, 2020-
Map releaseJan 15, 2020-
UpdateMar 6, 2024-
Current statusMar 6, 2024Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6v8o
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_21107.png

Downloads & links

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Map

FileDownload / File: emd_21107.map.gz / Format: CCP4 / Size: 91.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationRSC core structure
Voxel sizeX=Y=Z: 1.439 Å
Density
Contour LevelBy AUTHOR: 0.03 / Movie #1: 0.03
Minimum - Maximum-0.12098607 - 0.23140849
Average (Standard dev.)-0.0000356088 (±0.0048622275)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions288288288
Spacing288288288
CellA=B=C: 414.432 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.4391.4391.439
M x/y/z288288288
origin x/y/z0.0000.0000.000
length x/y/z414.432414.432414.432
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS288288288
D min/max/mean-0.1210.231-0.000

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Supplemental data

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Mask #1

Fileemd_21107_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: RSC core structure

Fileemd_21107_half_map_1.map
AnnotationRSC core structure
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: RSC core structure

Fileemd_21107_half_map_2.map
AnnotationRSC core structure
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Remodeling the Structure of Chromatin (RSC)

EntireName: Remodeling the Structure of Chromatin (RSC)
Components
  • Complex: Remodeling the Structure of Chromatin (RSC)
    • Protein or peptide: High temperature lethal protein 1
    • Protein or peptide: Chromatin structure-remodeling complex protein RSC14
    • Protein or peptide: Chromatin structure-remodeling complex subunit RSC7
    • Protein or peptide: Chromatin structure-remodeling complex subunit RSC2
    • Protein or peptide: Chromatin structure-remodeling complex protein RSC3
    • Protein or peptide: Chromatin structure-remodeling complex subunit RSC4
    • Protein or peptide: Chromatin structure-remodeling complex protein RSC8
    • Protein or peptide: Chromatin structure-remodeling complex protein RSC6
    • Protein or peptide: Chromatin structure-remodeling complex subunit RSC9
    • Protein or peptide: Chromatin structure-remodeling complex protein RSC58
    • Protein or peptide: Chromatin structure-remodeling complex subunit SFH1
    • Protein or peptide: Nuclear protein STH1/NPS1
    • Protein or peptide: Chromatin structure-remodeling complex protein RSC30
    • Protein or peptide: Unknown protein
    • Protein or peptide: Unknown Protein
    • Protein or peptide: Unknown protein
    • Protein or peptide: Unknown Protein
    • Protein or peptide: Unknown Protein
  • Ligand: ZINC ION

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Supramolecule #1: Remodeling the Structure of Chromatin (RSC)

SupramoleculeName: Remodeling the Structure of Chromatin (RSC) / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#18
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Molecular weightTheoretical: 1.1 MDa

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Macromolecule #1: High temperature lethal protein 1

MacromoleculeName: High temperature lethal protein 1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 9.192524 KDa
SequenceString:
MSQNNTISSM NPERAYNNVT LKNLTAFQLL SQRENICELL NLVESTERHN SIINPERQRM SLEEMKKMLD ALKNERKK

UniProtKB: High temperature lethal protein 1

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Macromolecule #2: Chromatin structure-remodeling complex protein RSC14

MacromoleculeName: Chromatin structure-remodeling complex protein RSC14 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 19.825398 KDa
SequenceString:
MSGSNMGYYD VLAGLSALEK SSQVVFSATE LQQLTQQSHA TDKGIEGSEN SKAKVSKPKR VAVHGYLGGK VSLADAAQVE YEVGHSLLG SYVPRQQLEA LSSVDFSHHF HRTLECKAAL ETHDVFLAGA GQLSLPFQSH IESPRNSEAK RKRKVIICKR C QSRFIGSH RRSQLREHAC VD

UniProtKB: Chromatin structure-remodeling complex protein RSC14

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Macromolecule #3: Chromatin structure-remodeling complex subunit RSC7

MacromoleculeName: Chromatin structure-remodeling complex subunit RSC7 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 49.71652 KDa
SequenceString: MSDSEGGLAS EVEHEKRSRS TSNRPNYAID TEDLDIDEND ENEDDDYREE EANEGVNEEE ISDEEEQINK SGRNKRRHVD EEEDLSEDK GVTRSRNRSK FKKPVFPGID DAEENLNPLK VVNEEYVLPD DPEGETKITA DGDLLGGREF LVRTFTLTEK G NRKFMLAT ...String:
MSDSEGGLAS EVEHEKRSRS TSNRPNYAID TEDLDIDEND ENEDDDYREE EANEGVNEEE ISDEEEQINK SGRNKRRHVD EEEDLSEDK GVTRSRNRSK FKKPVFPGID DAEENLNPLK VVNEEYVLPD DPEGETKITA DGDLLGGREF LVRTFTLTEK G NRKFMLAT EPARIVGFRD SYLFFQTHPN LYKFILNQTQ KNDLIDRGVL PYSYRNRQIA LVTARGVFKE FGAKIIRGGK HI TDDYYAS ELRTKGNVIE GKLAGDPIDK SARALETMMY PASENGINPA KNQVEFFEHR PHGHMSNSNI IASGSKLSST NWL YQHSAA CSRFNSDLFY DRVKVLLVDQ QGLRDAYTNI LHIPESTQST TVLGWRRSKN DSPSDTSIVY ETVIHDNDLN KPKT GLSEI PKEIYEDVVD EDVLRAITEQ QNFEKCNEYI

UniProtKB: Chromatin structure-remodeling complex subunit RSC7

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Macromolecule #4: Chromatin structure-remodeling complex subunit RSC2

MacromoleculeName: Chromatin structure-remodeling complex subunit RSC2 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 102.443664 KDa
SequenceString: MMPDDNSNSS TQNSSALYKD LRKEYESLFT LKEDSGLEIS PIFNVLPPKK DYPDYYAVIK NPVSFNTLKK RIPHYTDAQQ FMNDVVQIP WNAKTYNTRD SGIYKYALVL EKYLKDTIYP NLKEKYPQLV YPDLGPLPDE PGYEEFQQKL REKAEEVARA N AARAESSS ...String:
MMPDDNSNSS TQNSSALYKD LRKEYESLFT LKEDSGLEIS PIFNVLPPKK DYPDYYAVIK NPVSFNTLKK RIPHYTDAQQ FMNDVVQIP WNAKTYNTRD SGIYKYALVL EKYLKDTIYP NLKEKYPQLV YPDLGPLPDE PGYEEFQQKL REKAEEVARA N AARAESSS SMNSTEAARR LRKTRTSVKR ESEPGTDTNN DEDYEATDMD IDNPKDADFP DLIRKPLINI NPYTRKPLRD NR STTPSHS GTPQPLGPRH RQVSRTQVKR GRPPIIDLPY IQRMKNVMKV LKKEVLDSGI GLTDLFERLP DRHRDANYYI MIA NPISLQ DINKKVKTRR YKTFQEFQND FNLMLTNFRI SHRGDPESIK ISNILEKTFT SLARFELSKP DRSFIPEGEL RYPL DEVIV NNISYHVGDW ALLRNQNDPQ KPIVGQIFRL WKTPDGKQWL NACWYYRPEQ TVHRVDRLFY KNEVMKTGQY RDHLV SNLV GKCYVIHFTR YQRGNPDMKL EGPLFVCEFR YNESDKIFNK IRTWKACLPE EIRDLDEATI PVNGRKFFKY PSPIRH LLP ANATPHDRVP EPTMGSPDAP PLVGAVYMRP KMQRDDLGEY ATSDDCPRYI IRPNDSPEEG QVDIETGTIT TNTPTAN AL PKTGYSSSKL SSLRYNRSSM SLENQNAIGQ QQIPLSRVGS PGAGGPLTVQ GLKQHQLQRL QQQQHQYQQQ KRSQASRY N IPTIIDDLTS QASRGNLGNI MIDAASSFVL PISITKNVDV LQRTDLHSQT KRSGREEMFP WKKTKGEILW FRGPSVIVN ERIINSGDPH LSLPLNRWFT TNKKRKLEYE EVEETMEDVT GKDKDDDGLE PDVENEKESL PGPFVLGLRP SAKFTAHRLS MLRPPSSSS

UniProtKB: Chromatin structure-remodeling complex subunit RSC2

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Macromolecule #5: Chromatin structure-remodeling complex protein RSC3

MacromoleculeName: Chromatin structure-remodeling complex protein RSC3 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 101.819938 KDa
SequenceString: MDIRGRKMKK PPACVQCRKR KIGCDRVKPI CGNCMKHNKM DCFYPDVPGQ YVPSSSSSSN TRQVANGPYL NSYYASRRVS KETAALLQK NPELASLEQI REYNTRLQLL NAQNQLNNRS SAANATLNQQ HTQYIPKSVP SLESKPVTSA NESSTPLNWV Q GPAIFHML ...String:
MDIRGRKMKK PPACVQCRKR KIGCDRVKPI CGNCMKHNKM DCFYPDVPGQ YVPSSSSSSN TRQVANGPYL NSYYASRRVS KETAALLQK NPELASLEQI REYNTRLQLL NAQNQLNNRS SAANATLNQQ HTQYIPKSVP SLESKPVTSA NESSTPLNWV Q GPAIFHML TSPYTQDEII NHEMNFLKGR LLELQEITGK KITGVNLDLK QDSSAQMQSS HSNRNQEEFL TIKKRKLSED GV TDGDGKP IPESERRPHL NEFKDLDPQF LDTNKVFNVF NSAISEEGRN RLWLLPKNIN KSSIFQIQYL IERDPFLFKF FND LNILIE TQFNGPLHDL VASRNSIERN SGISQILKFP SQSITQTLIN KYLSTITETN SILPILKPKR LLPIVEQLFP SNTI NKPNS KDFETIFQVF SVTNDQLLNL GFITLCLLIL FESLNSTVLI PLRDDEHLQL FNVLFNYLPL LKSNLTTLRF EIEKR SMCN IETLRFISLW KYYQFVMDTS SSSSFVIDYD EDMHMACLLS LNHETQNQSH ILTWNFIFKN YCWRHLFLGQ LPLLMS EPF TNSTPIIDPL LNNDFELIDF EVNLMKYLQS KDQQLSIDKI IQLIKLLKNK NIEVSQGCLT TPSIINNIMD SLIYRNS ML YLNFYLLLQF ETLKNYAKFN EILEDFLELS RETLFFVFSN LANIKFAGHE FTFINKSIVV LQTLVLMLLA LYQRSFDS S KRTNDANEIS EQTDIHSNND NSKRIKNKNV IHLIINKIAM LLSDYSKNCK KQNKLIENLI IKIKTISKYI KNLEENKVT TSADSNYSIN NGFSGISAEQ LIKLNHELSK ISESLIKTDF YEQRKNSTVS NGVLGAAAPV DSDANSDTFG LTKENFNEVF EAIRS

UniProtKB: Chromatin structure-remodeling complex protein RSC3

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Macromolecule #6: Chromatin structure-remodeling complex subunit RSC4

MacromoleculeName: Chromatin structure-remodeling complex subunit RSC4 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 72.372375 KDa
SequenceString: MVVKKRKLAT EAGGSDERPK YLPGKHPKNQ EKTPHVDYNA PLNPKSELFL DDWHIPKFNR FISFTLDVLI DKYKDIFKDF IKLPSRKFH PQYYYKIQQP MSINEIKSRD YEYEDGPSNF LLDVELLTKN CQAYNEYDSL IVKNSMQVVM LIEFEVLKAK N LKRNYLIN ...String:
MVVKKRKLAT EAGGSDERPK YLPGKHPKNQ EKTPHVDYNA PLNPKSELFL DDWHIPKFNR FISFTLDVLI DKYKDIFKDF IKLPSRKFH PQYYYKIQQP MSINEIKSRD YEYEDGPSNF LLDVELLTKN CQAYNEYDSL IVKNSMQVVM LIEFEVLKAK N LKRNYLIN SEVKAKLLHY LNKLVDATEK KINQALLGAS SPKNLDDKVK LSEPFMELVD KDELPEYYEI VHSPMALSIV KQ NLEIGQY SKIYDFIIDM LLVFQNAHIF NDPSALIYKD ATTLTNYFNY LIQKEFFPEL QDLNERGEIN LEFDKFEFEN YLA IGGGGP AAAGALAISA LDNDIEPESN REDLIDQADY DFNHFEGLGN GYNRSLLTED YLLNPNNFKK LIAKPETVQS EVKN ERSTT SDIEKTNSLE SEHLKIPKYN VIKSMQKEMQ SLSEQHTMEY KPYKLIQQIY IFSSKNLYSQ ATKPLLGSRP SCNQN WVEY IFNGNELSQN ENAFSFMLQP MQTFLTLQSH LTSSLKDTET LLTINKEPVK SRTSNVNSNL SQPQQQENDV IGNDTK QDI ENLTIGGGNN NDIVGNDNDK RNNITEIFDI RLSEGLNHLM FRCEDKISHE TEFMNFWINV LP

UniProtKB: Chromatin structure-remodeling complex subunit RSC4

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Macromolecule #7: Chromatin structure-remodeling complex protein RSC8

MacromoleculeName: Chromatin structure-remodeling complex protein RSC8 / type: protein_or_peptide / ID: 7 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 63.253965 KDa
SequenceString: MSDTEKDKDV PMVDSHEATE EPPTTSTNTP SFPHLAQEQA KEESATLGAE VAHKKINYEQ EAQKLEEKAL RFLAKQTHPV IIPSFASWF DISKIHEIEK RSNPDFFNDS SRFKTPKAYK DTRNFIINTY RLSPYEYLTI TAVRRNVAMD VASIVKIHAF L EKWGLINY ...String:
MSDTEKDKDV PMVDSHEATE EPPTTSTNTP SFPHLAQEQA KEESATLGAE VAHKKINYEQ EAQKLEEKAL RFLAKQTHPV IIPSFASWF DISKIHEIEK RSNPDFFNDS SRFKTPKAYK DTRNFIINTY RLSPYEYLTI TAVRRNVAMD VASIVKIHAF L EKWGLINY QIDPRTKPSL IGPSFTGHFQ VVLDTPQGLK PFLPENVIKQ EVEGGDGAEP QVKKEFPVNL TIKKNVYDSA QD FNALQDE SRNSRQIHKV YICHTCGNES INVRYHNLRA RDTNLCSRCF QEGHFGANFQ SSDFIRLENN GNSVKKNWSD QEM LLLLEG IEMYEDQWEK IADHVGGHKR VEDCIEKFLS LPIEDNYIRE VVGSTLNGKG GDSRDGSVSG SKLMECVNDA VQTL LQGDD KLGKVSDKSR EISEKYIEES QAIIQELVKL TMEKLESKFT KLCDLETQLE MEKLKYVKES EKMLNDRLSL SKQIL DLNK SLEELNVSKK LVLISEQVDS GIQLVEKDQE GDDEDGNTAT GHGVKRVGKE GEEVGEGDSI AKLQPQVYKP WSL

UniProtKB: Chromatin structure-remodeling complex protein RSC8

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Macromolecule #8: Chromatin structure-remodeling complex protein RSC6

MacromoleculeName: Chromatin structure-remodeling complex protein RSC6 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 54.222691 KDa
SequenceString: MVTQTNPVPV TYPTDAYIPT YLPDDKVSNL ADLKKLIEMD SRLDLYLTRR RLDTSINLPT NTKTKDHPPN KEMLRIYVYN TTESSPRSD SGTPADSGKT TWTLRIEGKL LHESANGKHP FSEFLEGVAV DFKRLKPLGM GKKRKRDSSL SLPLNLQQPE Y NDQDSTMG ...String:
MVTQTNPVPV TYPTDAYIPT YLPDDKVSNL ADLKKLIEMD SRLDLYLTRR RLDTSINLPT NTKTKDHPPN KEMLRIYVYN TTESSPRSD SGTPADSGKT TWTLRIEGKL LHESANGKHP FSEFLEGVAV DFKRLKPLGM GKKRKRDSSL SLPLNLQQPE Y NDQDSTMG DNDNGEDEDS AEAESREEIV DALEWNYDEN NVVEFDGIDI KRQGKDNLRC SITIQLRGVD GGKVQYSPNL AT LIGMQTG SVNDAVYSIY KYILINNLFV TEQTEAQDGS NDAEDSSNEN NNKNGAGDDD GVEGSTPKDK PELGEVKLDS LLQ KVLDTN AAHLPLMNVV QTVNKLVSPL PPIILDYTID LSKDTTYGAT TLDVDVSHIL HQPQPQPNLQ KEEETDAEDT AKLR EITKL ALQLNSSAQK YQFFHELSLH PRETLTHYLW SSKQNELVLQ GDQYFNEDAA RTSDIYSNNN NDRSLMGNIS LLYSQ GRL

UniProtKB: Chromatin structure-remodeling complex protein RSC6

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Macromolecule #9: Chromatin structure-remodeling complex subunit RSC9

MacromoleculeName: Chromatin structure-remodeling complex subunit RSC9 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 65.289309 KDa
SequenceString: MNSLASNTPL NGTPVSEAPA TSSEPVNMFE TMVANPIKVS RLQSNGVLTG PAANTKSIHY SLANFNVFQS LPKETARGVD DLTRMEMAL LSGIPEEIKW SLKKYLTYSN KAPYMISLRT LPDLLPLFKT FILPLERIVE GLNKSSICDS KAMDSLQMGL N ALLILRNL ...String:
MNSLASNTPL NGTPVSEAPA TSSEPVNMFE TMVANPIKVS RLQSNGVLTG PAANTKSIHY SLANFNVFQS LPKETARGVD DLTRMEMAL LSGIPEEIKW SLKKYLTYSN KAPYMISLRT LPDLLPLFKT FILPLERIVE GLNKSSICDS KAMDSLQMGL N ALLILRNL AQDTDSVQIL VKDREIKSFI LFILKKFQCV ATGDNKWQLY EGNATFFNEL THYTLDLMEA ISSYIAPAMK DD HYFQTLV SILNYTKDRY MVISILRSLS RLLVRSKANE ESAADNLDHK TLSLIVSFLL LECDSELIIA SLDFLYQYIL PGS QRITEL FKSKECSLIL EATLPNLLSY NIATPDYHLL QKHKIRLIKR LKPPAPKEPP NLSEDLFQQL FKLNEPLRST AWLR CCFEP VQEAEFTQIS LWRSYESKFG QPVRESGRKL LPAVEFIKNV SNAFNNAAAI VITDPVTGKK RFVIKGIQPR FKALG IADG ERESQVPISA LKSKFLNDSK EITPARQNSI PEVKFPQELS DVSKVACTFL CLLSNDTDDG AGSAFCQRIR PLVLHK LAD IPPLTLALSE YMENTSGL

UniProtKB: Chromatin structure-remodeling complex subunit RSC9

+
Macromolecule #10: Chromatin structure-remodeling complex protein RSC58

MacromoleculeName: Chromatin structure-remodeling complex protein RSC58 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 57.871309 KDa
SequenceString: MTESVGGNKL VDFLVNVQSI LNAASVKCHV VDESFPAKFF EKNPDKIYES YCKFIKNRSN SEGLIRNEDK LVLTTINKRF ENGEYEPIQ GGFYKLYHDI KLVCTILIHF YPQGTRNYQL VDKFYKFSSE LLLRECCRIG IALTQTNNIK SRSGKLLSGN E MDEYDDDD ...String:
MTESVGGNKL VDFLVNVQSI LNAASVKCHV VDESFPAKFF EKNPDKIYES YCKFIKNRSN SEGLIRNEDK LVLTTINKRF ENGEYEPIQ GGFYKLYHDI KLVCTILIHF YPQGTRNYQL VDKFYKFSSE LLLRECCRIG IALTQTNNIK SRSGKLLSGN E MDEYDDDD ATELDKIISY DFIKISMNYT VPISQTYQIR TKDMDLFSSI ISKSNLDKRP HELPNTNFKI NNVLPQTDIE NE APRLGFV GANTSNIPDP TLPPTEMMTR FLHPNWYALP TTVWLKYGNY NSWAPSFNEN GTVVDSTTRG LIWLERIGYM DLY EKNEKK VKQEELLNTN EEGINRKQND ENNKNVDGKS NGVQDDGGDN DNDATIASAN SESTENKEQF IIKLQNLYNW TPSN YIGDD EIENFRNGTP DKLVSDSLLK LKRLRKERIL NKVLKPTTEE RELYFKVKRI LKEVILAKKV SKVPINNVRA FPVLQ TNYN GSIPVVRAQP GRKRKHKK

UniProtKB: Chromatin structure-remodeling complex protein RSC58

+
Macromolecule #11: Chromatin structure-remodeling complex subunit SFH1

MacromoleculeName: Chromatin structure-remodeling complex subunit SFH1 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 48.83318 KDa
SequenceString: MSHQNQLIPQ AYISNFHNRL TNEDDGIPIF TMAQQTRQHK RAKVVNYAEY DNDLFDEFNM NGSNFNNADT HYKDNAVSHE NTPALTNGV TMDGSEYNVL ENMNGADSII SNNKYDAGSN MVVESLSGLN SNNNASNGPS NKAQAQDIGN AVLPDLQDQH H NPFNILRY ...String:
MSHQNQLIPQ AYISNFHNRL TNEDDGIPIF TMAQQTRQHK RAKVVNYAEY DNDLFDEFNM NGSNFNNADT HYKDNAVSHE NTPALTNGV TMDGSEYNVL ENMNGADSII SNNKYDAGSN MVVESLSGLN SNNNASNGPS NKAQAQDIGN AVLPDLQDQH H NPFNILRY PKIRDTFING KVVSPYRLNT DQETKANANS GEAIMIPITL DIEHMGHTIK DQFLWNYNDD SISPEEFASI YC KDLDMTS ATLQTQIANI IKEQLKDLEN IAATEIMSDL HVIINLTCNL QDRFFEDNFQ WNLNDKSLTP ERFATSIVQD LGL TREFIP LISQSLHETI LKIKKDWVDG HLIQDHVPND AAFGYLSGIR LDIDELGSNW CPRVEILTKE EIQKREIEKE RNLR RLKRE TDRLSRRGRR RLDDLETTMR M

UniProtKB: Chromatin structure-remodeling complex subunit SFH1

+
Macromolecule #12: Nuclear protein STH1/NPS1

MacromoleculeName: Nuclear protein STH1/NPS1 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 156.982406 KDa
SequenceString: MLQEQSELMS TVMNNTPTTV AALAAVAAAS ETNGKLGSEE QPEITIPKPR SSAQLEQLLY RYRAIQNHPK ENKLEIKAIE DTFRNISRD QDIYETKLDT LRKSIDKGFQ YDEDLLNKHL VALQLLEKDT DVPDYFLDLP DTKNDNTTAI EVDYSEKKPI K ISADFNAK ...String:
MLQEQSELMS TVMNNTPTTV AALAAVAAAS ETNGKLGSEE QPEITIPKPR SSAQLEQLLY RYRAIQNHPK ENKLEIKAIE DTFRNISRD QDIYETKLDT LRKSIDKGFQ YDEDLLNKHL VALQLLEKDT DVPDYFLDLP DTKNDNTTAI EVDYSEKKPI K ISADFNAK AKSLGLESKF SNATKTALGD PDTEIRISAR ISNRINELER LPANLGTYSL DDCLEFITKD DLSSRMDTFK IK ALVELKS LKLLTKQKSI RQKLINNVAS QAHHNIPYLR DSPFTAAAQR SVQIRSKVIV PQTVRLAEEL ERQQLLEKRK KER NLHLQK INSIIDFIKE RQSEQWSRQE RCFQFGRLGA SLHNQMEKDE QKRIERTAKQ RLAALKSNDE EAYLKLLDQT KDTR ITQLL RQTNSFLDSL SEAVRAQQNE AKILHGEEVQ PITDEEREKT DYYEVAHRIK EKIDKQPSIL VGGTLKEYQL RGLEW MVSL YNNHLNGILA DEMGLGKTIQ SISLITYLYE VKKDIGPFLV IVPLSTITNW TLEFEKWAPS LNTIIYKGTP NQRHSL QHQ IRVGNFDVLL TTYEYIIKDK SLLSKHDWAH MIIDEGHRMK NAQSKLSFTI SHYYRTRNRL ILTGTPLQNN LPELWAL LN FVLPKIFNSA KTFEDWFNTP FANTGTQEKL ELTEEETLLI IRRLHKVLRP FLLRRLKKEV EKDLPDKVEK VIKCKLSG L QQQLYQQMLK HNALFVGAGT EGATKGGIKG LNNKIMQLRK ICNHPFVFDE VEGVVNPSRG NSDLLFRVAG KFELLDRVL PKFKASGHRV LMFFQMTQVM DIMEDFLRMK DLKYMRLDGS TKTEERTEML NAFNAPDSDY FCFLLSTRAG GLGLNLQTAD TVIIFDTDW NPHQDLQAQD RAHRIGQKNE VRILRLITTD SVEEVILERA MQKLDIDGKV IQAGKFDNKS TAEEQEAFLR R LIESETNR DDDDKAELDD DELNDTLARS ADEKILFDKI DKERMNQERA DAKAQGLRVP PPRLIQLDEL PKVFREDIEE HF KKEDSEP LGRIRQKKRV YYDDGLTEEQ FLEAVEDDNM SLEDAIKKRR EARERRRLRQ NGTKENEIET LENTPEASET SLI ENNSFT AAVDEETNAD KETTASRSKR RSSRKKRTIS IVTAEDKENT QEESTSQENG GAKVEEEVKS SSVEIINGSE SKKK KPKLT VKIKLNKTTV LENNDGKRAE EKPESKSPAK KTAAKKTKTK SKSLGIFPTV EKLVEEMREQ LDEVDSHPRT SIFEK LPSK RDYPDYFKVI EKPMAIDIIL KNCKNGTYKT LEEVRQALQT MFENARFYNE EGSWVYVDAD KLNEFTDEWF KEHSS

UniProtKB: Nuclear protein STH1/NPS1

+
Macromolecule #13: Chromatin structure-remodeling complex protein RSC30

MacromoleculeName: Chromatin structure-remodeling complex protein RSC30 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 101.448211 KDa
SequenceString: MMDMQVRKVR KPPACTQCRK RKIGCDRAKP ICGNCVKYNK PDCFYPDGPG KMVAVPSASG MSTHGNGQGS NHFSQGNGVN QKNVMIQTQ YPIMQTSIEA FNFSFNPSVD TAMQWTKAAS YQNNNTNNNT APRQNSSTVS SNVHGNTIVR SDSPDVPSMD Q IREYNTRL ...String:
MMDMQVRKVR KPPACTQCRK RKIGCDRAKP ICGNCVKYNK PDCFYPDGPG KMVAVPSASG MSTHGNGQGS NHFSQGNGVN QKNVMIQTQ YPIMQTSIEA FNFSFNPSVD TAMQWTKAAS YQNNNTNNNT APRQNSSTVS SNVHGNTIVR SDSPDVPSMD Q IREYNTRL QLVNAQSFDY TDNPYSFNVG INQDSAVFDL MTSPFTQEEV LIKEIDFLKN KLLDLQSLQL KSLKEKSNLN AD NTTANKI NKTGENSKKG KVDGKRAGFD HQTSRTSQSS QKYFTALTIT DVQSLVQVKP LKDTPNYLFT KNFIIFRDHY LFK FYNILH DICHINQFKV SPPNNKNHQQ YMEVCKVNFP PKAIIIETLN SESLNNLNIE EFLPIFDKTL LLEFVHNSFP NGDT CPSFS TVDLPLSQLT KLGELTVLLL LLNDSMTLFN KQAINNHVSA LMNNLRLIRS QITLINLEYY DQETIKFIAI TKFYE SLYM HDDHKSSLDE DLSCLLSFQI KDFKLFHFLK KMYYSRHSLL GQSSFMVPAA ENLSPIPASI DTNDIPLIAN DLKLLE TQA KLINILQGVP FYLPVNLTKI ESLLETLTMG VSNTVDLYFH DNEVRKEWKD TLNFINTIVY TNFFLFVQNE SSLSMAV QH SSNNNKTSNS ERCAKDLMKI ISNMHIFYSI TFNFIFPIKS IKSFSSGNNR FHSNGKEFLF ANHFIEILQN FIAITFAI F QRCEVILYDE FYKNLSNEEI NVQLLLIHDK ILEILKKIEI IVSFLRDEMN SNGSFKSIKG FNKVLNLIKY MLRFSKKKQ NFARNSDNNN VTDYSQSAKN KNVLLKFPVS ELNRIYLKFK EISDFLMERE VVQRSIIIDK DLESDNLGIT TANFNDFYDA FYN

UniProtKB: Chromatin structure-remodeling complex protein RSC30

+
Macromolecule #14: Unknown protein

MacromoleculeName: Unknown protein / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 2.400951 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

+
Macromolecule #15: Unknown Protein

MacromoleculeName: Unknown Protein / type: protein_or_peptide / ID: 15 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 1.635006 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

+
Macromolecule #16: Unknown protein

MacromoleculeName: Unknown protein / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 1.209482 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

+
Macromolecule #17: Unknown Protein

MacromoleculeName: Unknown Protein / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 1.294587 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

+
Macromolecule #18: Unknown Protein

MacromoleculeName: Unknown Protein / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 4.188154 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

+
Macromolecule #19: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 19 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.9
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 40.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Startup modelType of model: OTHER
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.07 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 1920066
FSC plot (resolution estimation)

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