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- EMDB-2093: Three Dimensional Structure of the Epstein-Barr Virus Capsid. -

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Basic information

Entry
Database: EMDB / ID: EMD-2093
TitleThree Dimensional Structure of the Epstein-Barr Virus Capsid.
Map dataEBV capsid purified over saccharose
Sample
  • Sample: Epstein-Barr capsid purified over saccharose gradient
  • Virus: Human herpesvirus 4 (Epstein-Barr virus)
KeywordsStructure / cryo-electron microscopy / Epstein-Barr virus / purification
Biological speciesHuman herpesvirus 4 (Epstein-Barr virus)
Methodsingle particle reconstruction / cryo EM / negative staining / Resolution: 19.6 Å
AuthorsGermi R / Effantin G / Grossi L / Ruigrok RWH / Morand P / Schoehn G
CitationJournal: J Gen Virol / Year: 2012
Title: Three-dimensional structure of the Epstein-Barr virus capsid.
Authors: Raphaele Germi / Gregory Effantin / Laurence Grossi / Rob W H Ruigrok / Patrice Morand / Guy Schoehn /
Abstract: Epstein-Barr virus (EBV), a gammaherpesvirus, infects >90 % of the world's population. Primary infection by EBV can lead to infectious mononucleosis, and EBV persistence is associated with several ...Epstein-Barr virus (EBV), a gammaherpesvirus, infects >90 % of the world's population. Primary infection by EBV can lead to infectious mononucleosis, and EBV persistence is associated with several malignancies. Despite its importance for human health, little structural information is available on EBV. Here we report the purification of the EBV capsid by CsCl- or sucrose density-gradient centrifugation. Cryo-electron microscopy and image analysis resulted in two slightly different three-dimensional structures at about 20 Å resolution. These structures were compared with that of human herpesvirus 8, another gammaherpesvirus. CsCl-gradient purification leads to the removal of part of the triplex complex around the fivefold axes, whereas the complexes between hexons remained in place. This may be due to local differences in stability resulting from variation in quasi-equivalent interactions between pentons and hexons compared with those between hexons only.
History
DepositionMay 4, 2012-
Header (metadata) releaseSep 26, 2012-
Map releaseSep 26, 2012-
UpdateSep 26, 2012-
Current statusSep 26, 2012Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 4
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 4
  • Imaged by UCSF Chimera
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Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

EMD-2093.png

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Map

FileDownload / File: emd_2093.map.gz / Format: CCP4 / Size: 127.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationEBV capsid purified over saccharose
Voxel sizeX=Y=Z: 4.52 Å
Density
Contour LevelBy AUTHOR: 3.8 / Movie #1: 4
Minimum - Maximum-32.205112460000002 - 26.601320269999999
Average (Standard dev.)0.04438958 (±3.16710472)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions325325325
Spacing325325325
CellA=B=C: 1469.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z4.524.524.52
M x/y/z325325325
origin x/y/z0.0000.0000.000
length x/y/z1469.0001469.0001469.000
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ128128168
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS325325325
D min/max/mean-32.20526.6010.044

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Supplemental data

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Sample components

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Entire : Epstein-Barr capsid purified over saccharose gradient

EntireName: Epstein-Barr capsid purified over saccharose gradient
Components
  • Sample: Epstein-Barr capsid purified over saccharose gradient
  • Virus: Human herpesvirus 4 (Epstein-Barr virus)

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Supramolecule #1000: Epstein-Barr capsid purified over saccharose gradient

SupramoleculeName: Epstein-Barr capsid purified over saccharose gradient / type: sample / ID: 1000 / Number unique components: 1

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Supramolecule #1: Human herpesvirus 4

SupramoleculeName: Human herpesvirus 4 / type: virus / ID: 1
Details: Peripentonal triplex keep in position during purification
NCBI-ID: 10376 / Sci species name: Human herpesvirus 4 / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: Yes / Virus empty: No
Host (natural)Organism: Homo sapiens (human) / synonym: VERTEBRATES
Virus shellShell ID: 1 / Name: EBV / Diameter: 1250 Å / T number (triangulation number): 16

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Experimental details

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Structure determination

Methodnegative staining, cryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4 / Details: 0.05 M Tris 0.15 M NaCl, pH 7.4
StainingType: NEGATIVE / Details: Cryo
GridDetails: Quantifoil 300 mesh R2/1
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV / Method: Blot for 1.5 second before plunging

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Electron microscopy

MicroscopeFEI POLARA 300
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.2 µm / Nominal magnification: 31000
Sample stageSpecimen holder model: GATAN LIQUID NITROGEN
Alignment procedureLegacy - Astigmatism: Objective lens astigmatism was corrected at 100,000 times magnification
DateJun 3, 2011
Image recordingCategory: FILM / Film or detector model: KODAK SO-163 FILM / Digitization - Scanner: ZEISS SCAI / Digitization - Sampling interval: 7 µm / Number real images: 36 / Average electron dose: 20 e/Å2 / Details: binned / Bits/pixel: 8
Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company

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Image processing

CTF correctionDetails: Each particle
Final reconstructionApplied symmetry - Point group: I (icosahedral) / Algorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 19.6 Å / Resolution method: OTHER / Software - Name: PFT2, em3dr2 / Number images used: 370
Detailspft2 and em3dr2

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