[English] 日本語
Yorodumi
- EMDB-16533: Cryo-EM structure of native Otu2-bound ubiquitinated 48S initiati... -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: EMDB / ID: EMD-16533
TitleCryo-EM structure of native Otu2-bound ubiquitinated 48S initiation complex (partial)
Map dataCryo-EM map of Otu2-bound ubiquitinated 48S initiation complex
Sample
  • Complex: Yeast native 48S initiation complex (partial) with deubiquitinating enzyme Otu2 bound to ubiquitinated eS7
    • Protein or peptide: x 47 types
    • RNA: x 3 types
  • Ligand: x 7 types
Function / homology
Function and homology information


eukaryotic translation initiation factor 3 complex, eIF3e / eukaryotic translation initiation factor 3 complex, eIF3m / translation reinitiation / incipient cellular bud site / multi-eIF complex / eukaryotic translation initiation factor 3 complex / eukaryotic 43S preinitiation complex / protein-synthesizing GTPase / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / formation of cytoplasmic translation initiation complex ...eukaryotic translation initiation factor 3 complex, eIF3e / eukaryotic translation initiation factor 3 complex, eIF3m / translation reinitiation / incipient cellular bud site / multi-eIF complex / eukaryotic translation initiation factor 3 complex / eukaryotic 43S preinitiation complex / protein-synthesizing GTPase / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / formation of cytoplasmic translation initiation complex / eukaryotic 48S preinitiation complex / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / protein metabolic process / Protein methylation / RMTs methylate histone arginines / positive regulation of translational fidelity / mTORC1-mediated signalling / ribosome-associated ubiquitin-dependent protein catabolic process / Protein hydroxylation / GDP-dissociation inhibitor activity / : / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / preribosome, small subunit precursor / mRNA destabilization / Major pathway of rRNA processing in the nucleolus and cytosol / SRP-dependent cotranslational protein targeting to membrane / 90S preribosome / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / ribosomal small subunit export from nucleus / L13a-mediated translational silencing of Ceruloplasmin expression / translation regulator activity / ribosomal large subunit export from nucleus / G-protein alpha-subunit binding / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of translational fidelity / positive regulation of protein kinase activity / rescue of stalled ribosome / translation initiation factor binding / maturation of SSU-rRNA / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translational initiation / DNA-(apurinic or apyrimidinic site) endonuclease activity / cellular response to amino acid starvation / ribosome assembly / translation initiation factor activity / small-subunit processome / protein kinase C binding / maintenance of translational fidelity / modification-dependent protein catabolic process / ribosomal small subunit biogenesis / ribosomal large subunit assembly / small ribosomal subunit rRNA binding / protein tag activity / ribosomal small subunit assembly / rRNA processing / cytoplasmic stress granule / cytosolic small ribosomal subunit / ribosome binding / ribosome biogenesis / small ribosomal subunit / cytoplasmic translation / cytosolic large ribosomal subunit / cysteine-type deubiquitinase activity / tRNA binding / negative regulation of translation / rRNA binding / protein ubiquitination / ribosome / structural constituent of ribosome / positive regulation of protein phosphorylation / translation / G protein-coupled receptor signaling pathway / negative regulation of gene expression / mRNA binding / GTPase activity / ubiquitin protein ligase binding / GTP binding / nucleolus / ATP hydrolysis activity / mitochondrion / RNA binding / zinc ion binding / nucleoplasm / ATP binding / metal ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
: / Eukaryotic translation initiation factor 3 subunit G / Eukaryotic translation initiation factor 3 subunit G, N-terminal / eIF3G, RNA recognition motif / Eukaryotic translation initiation factor 3 subunit G / Eukaryotic translation initiation factor 3 subunit I / RLI, domain 1 / RLI1 / RNase L inhibitor RLI-like, possible metal-binding domain / Eukaryotic translation initiation factor 3 subunit B ...: / Eukaryotic translation initiation factor 3 subunit G / Eukaryotic translation initiation factor 3 subunit G, N-terminal / eIF3G, RNA recognition motif / Eukaryotic translation initiation factor 3 subunit G / Eukaryotic translation initiation factor 3 subunit I / RLI, domain 1 / RLI1 / RNase L inhibitor RLI-like, possible metal-binding domain / Eukaryotic translation initiation factor 3 subunit B / eIF3B, RNA recognition motif / Possible Fer4-like domain in RNase L inhibitor, RLI / Eukaryotic translation initiation factor 3 subunit C, N-terminal domain / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor 3 subunit 8 N-terminus / Translation initiation factor, beta propellor-like domain / Eukaryotic translation initiation factor 3 subunit A / OTU-like cysteine protease / Eukaryotic translation initiation factor eIF2A / Translation initiation factor IF2/IF5 / Translation initiation factor IF2/IF5 domain / Translation initiation factor IF2/IF5, N-terminal / Translation initiation factor IF2/IF5, zinc-binding / Domain found in IF2B/IF5 / domain present in translation initiation factor eIF2B and eIF5 / Translation initiation factor 1A (eIF-1A), conserved site / Eukaryotic initiation factor 1A signature. / OTU domain / OTU domain profile. / eukaryotic translation initiation factor 1A / Translation initiation factor 1A (eIF-1A) / eIF4-gamma/eIF5/eIF2-epsilon / Domain at the C-termini of GCD6, eIF-2B epsilon, eIF-4 gamma and eIF-5 / W2 domain / W2 domain profile. / IF2a, S1-like domain / Translation initiation factor 2, alpha subunit / Translation initiation factor 2, alpha subunit, middle domain superfamily / Translation initiation factor 2, alpha subunit, C-terminal / Eukaryotic translation initiation factor 2 alpha subunit / Initiation factor eIF2 gamma, domain 2 / Initiation factor eIF2 gamma, GTP-binding domain / Initiation factor eIF2 gamma, C-terminal / Initiation factor eIF2 gamma, C terminal / RNA-binding domain, S1, IF1 type / Translation initiation factor 1A / IF-1 / S1 domain IF1 type profile. / 4Fe-4S binding domain / RNA-binding domain, S1 / PCI/PINT associated module / motif in proteasome subunits, Int-6, Nip-1 and TRIP-15 / PCI domain / Proteasome component (PCI) domain / PCI domain profile. / Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal / : / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S12e / : / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S21e, conserved site / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S19e, conserved site / S27a-like superfamily / Ribosomal protein S26e / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S25 / Ribosomal protein S26e signature. / S1 domain profile. / Ribosomal protein S17e, conserved site / : / Ribosomal protein S2, eukaryotic / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S8e subdomain, eukaryotes / S25 ribosomal protein / Ribosomal protein S19A/S15e / Ribosomal protein S21e signature. / Ribosomal protein S30 / Ribosomal protein S3Ae, conserved site / Ribosomal protein S12e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S8e, conserved site / 40S ribosomal protein S11, N-terminal / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S6, eukaryotic
Similarity search - Domain/homology
Eukaryotic translation initiation factor 3 subunit I / Eukaryotic translation initiation factor 4C / SUI2 isoform 1 / protein-synthesizing GTPase / RLI1 isoform 1 / TIF5 isoform 1 / SUI3 isoform 1 / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor 3 subunit B / Eukaryotic translation initiation factor 3 subunit G ...Eukaryotic translation initiation factor 3 subunit I / Eukaryotic translation initiation factor 4C / SUI2 isoform 1 / protein-synthesizing GTPase / RLI1 isoform 1 / TIF5 isoform 1 / SUI3 isoform 1 / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor 3 subunit B / Eukaryotic translation initiation factor 3 subunit G / Small ribosomal subunit protein uS4A / Small ribosomal subunit protein eS17A / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS15 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein uS11A / Small ribosomal subunit protein eS19A / Small ribosomal subunit protein eS21A / Small ribosomal subunit protein uS8A / Ubiquitin-ribosomal protein eL40A fusion protein / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A / Small ribosomal subunit protein eS30A / Small ribosomal subunit protein eS4B / Small ribosomal subunit protein eS6B / Small ribosomal subunit protein eS8A / Small ribosomal subunit protein uS17A / Small ribosomal subunit protein uS9A / Small ribosomal subunit protein uS13A / Small ribosomal subunit protein eS32A / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS7A / Small ribosomal subunit protein uS2A / Small ribosomal subunit protein eS1A / Small ribosomal subunit protein eS27A / Small ribosomal subunit protein RACK1 / Eukaryotic translation initiation factor 3 subunit A / Small ribosomal subunit protein uS10 / OTU domain-containing protein 2 / Small ribosomal subunit protein eS26A / Small ribosomal subunit protein uS14A / Small ribosomal subunit protein eS12 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein eS10A / Small ribosomal subunit protein eS25A / Small ribosomal subunit protein eS28A
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast) / Saccharomyces cerevisiae W303 (yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.3 Å
AuthorsIkeuchi K / Buschauer R / Cheng J / Berninghausen O / Becker T / Beckmann R
Funding support Germany, Japan, 5 items
OrganizationGrant numberCountry
German Research Foundation (DFG)SFB/TRR-174 Germany
German Research Foundation (DFG)BE1814/15-1 Germany
German Research Foundation (DFG)BE1814/1-1 Germany
Japan Society for the Promotion of Science (JSPS)JSPS Overseas Research Fellowships Japan
Boehringer Ingelheim Fonds (BIF)PhD fellowship Germany
CitationJournal: Nat Commun / Year: 2023
Title: Molecular basis for recognition and deubiquitination of 40S ribosomes by Otu2.
Authors: Ken Ikeuchi / Nives Ivic / Robert Buschauer / Jingdong Cheng / Thomas Fröhlich / Yoshitaka Matsuo / Otto Berninghausen / Toshifumi Inada / Thomas Becker / Roland Beckmann /
Abstract: In actively translating 80S ribosomes the ribosomal protein eS7 of the 40S subunit is monoubiquitinated by the E3 ligase Not4 and deubiquitinated by Otu2 upon ribosomal subunit recycling. Despite its ...In actively translating 80S ribosomes the ribosomal protein eS7 of the 40S subunit is monoubiquitinated by the E3 ligase Not4 and deubiquitinated by Otu2 upon ribosomal subunit recycling. Despite its importance for translation efficiency the exact role and structural basis for this translational reset is poorly understood. Here, structural analysis by cryo-electron microscopy of native and reconstituted Otu2-bound ribosomal complexes reveals that Otu2 engages 40S subunits mainly between ribosome recycling and initiation stages. Otu2 binds to several sites on the intersubunit surface of the 40S that are not occupied by any other 40S-binding factors. This binding mode explains the discrimination against 80S ribosomes via the largely helical N-terminal domain of Otu2 as well as the specificity for mono-ubiquitinated eS7 on 40S. Collectively, this study reveals mechanistic insights into the Otu2-driven deubiquitination steps for translational reset during ribosome recycling/(re)initiation.
History
DepositionJan 24, 2023-
Header (metadata) releaseMay 24, 2023-
Map releaseMay 24, 2023-
UpdateMay 24, 2023-
Current statusMay 24, 2023Processing site: PDBe / Status: Released

-
Structure visualization

Supplemental images

emd_16533.png

Downloads & links

-
Map

FileDownload / File: emd_16533.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationCryo-EM map of Otu2-bound ubiquitinated 48S initiation complex
Voxel sizeX=Y=Z: 1.045 Å
Density
Contour LevelBy AUTHOR: 0.1
Minimum - Maximum-1.8571861 - 3.5959535
Average (Standard dev.)0.006660883 (±0.080036215)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions420420420
Spacing420420420
CellA=B=C: 438.9 Å
α=β=γ: 90.0 °

-
Supplemental data

-
Half map: Half-map A

Fileemd_16533_half_map_1.map
AnnotationHalf-map A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

-
Half map: Half-map B

Fileemd_16533_half_map_2.map
AnnotationHalf-map B
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

-
Sample components

+
Entire : Yeast native 48S initiation complex (partial) with deubiquitinati...

EntireName: Yeast native 48S initiation complex (partial) with deubiquitinating enzyme Otu2 bound to ubiquitinated eS7
Components
  • Complex: Yeast native 48S initiation complex (partial) with deubiquitinating enzyme Otu2 bound to ubiquitinated eS7
    • Protein or peptide: 40S ribosomal protein S0-ARibosome
    • Protein or peptide: 40S ribosomal protein S1-ARibosome
    • Protein or peptide: 40S ribosomal protein S2
    • Protein or peptide: 40S ribosomal protein S4-BRibosome
    • Protein or peptide: 40S ribosomal protein S6-BRibosome
    • Protein or peptide: 40S ribosomal protein S8-ARibosome
    • Protein or peptide: 40S ribosomal protein S9-ARibosome
    • Protein or peptide: 40S ribosomal protein S11-ARibosome
    • Protein or peptide: 40S ribosomal protein S13
    • Protein or peptide: 40S ribosomal protein S14-ARibosome
    • Protein or peptide: 40S ribosomal protein S21-ARibosome
    • Protein or peptide: 40S ribosomal protein S22-ARibosome
    • Protein or peptide: 40S ribosomal protein S23-ARibosome
    • Protein or peptide: 40S ribosomal protein S24-ARibosome
    • Protein or peptide: 40S ribosomal protein S26-ARibosome
    • Protein or peptide: 40S ribosomal protein S27-ARibosome
    • Protein or peptide: 40S ribosomal protein S30-ARibosome
    • Protein or peptide: RPS15 isoform 1
    • Protein or peptide: 40S ribosomal protein S3
    • Protein or peptide: 40S ribosomal protein S10-ARibosome
    • Protein or peptide: 40S ribosomal protein S12
    • Protein or peptide: 40S ribosomal protein S16-ARibosome
    • Protein or peptide: 40S ribosomal protein S17-ARibosome
    • Protein or peptide: 40S ribosomal protein S18-ARibosome
    • Protein or peptide: 40S ribosomal protein S19-ARibosome
    • Protein or peptide: 40S ribosomal protein S20
    • Protein or peptide: 40S ribosomal protein S25-ARibosome
    • Protein or peptide: RPS29A isoform 1
    • Protein or peptide: 40S ribosomal protein S31
    • Protein or peptide: Guanine nucleotide-binding protein subunit beta-like protein
    • Protein or peptide: 40S ribosomal protein S28-ARibosome
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit IEukaryotic initiation factor 3
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit GEukaryotic initiation factor 3
    • RNA: tRNATransfer RNA
    • Protein or peptide: TIF5 isoform 1
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit AEukaryotic initiation factor 3
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit BEukaryotic initiation factor 3
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit CEukaryotic initiation factor 3
    • RNA: mRNAMessenger RNA
    • Protein or peptide: Eukaryotic translation initiation factor 4C
    • Protein or peptide: RLI1 isoform 1
    • RNA: 18S ribosomal RNA
    • Protein or peptide: 40S ribosomal protein S5
    • Protein or peptide: 60S ribosomal protein L40-ARibosome
    • Protein or peptide: 60S ribosomal protein L41-ARibosome
    • Protein or peptide: SUI2 isoform 1
    • Protein or peptide: protein-synthesizing GTPase
    • Protein or peptide: SUI3 isoform 1
    • Protein or peptide: OTU domain-containing protein 2
    • Protein or peptide: 40S ribosomal protein S7-ARibosome
  • Ligand: ZINC ION
  • Ligand: ADENOSINE-5'-DIPHOSPHATE
  • Ligand: MAGNESIUM ION
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: IRON/SULFUR CLUSTERIron–sulfur cluster
  • Ligand: METHIONINE
  • Ligand: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER

+
Supramolecule #1: Yeast native 48S initiation complex (partial) with deubiquitinati...

SupramoleculeName: Yeast native 48S initiation complex (partial) with deubiquitinating enzyme Otu2 bound to ubiquitinated eS7
type: complex / ID: 1 / Chimera: Yes / Parent: 0 / Macromolecule list: #1-#50
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: W303

+
Macromolecule #1: 40S ribosomal protein S0-A

MacromoleculeName: 40S ribosomal protein S0-A / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 28.05133 KDa
SequenceString: MSLPATFDLT PEDAQLLLAA NTHLGARNVQ VHQEPYVFNA RPDGVHVINV GKTWEKLVLA ARIIAAIPNP EDVVAISSRT FGQRAVLKF AAHTGATPIA GRFTPGSFTN YITRSFKEPR LVIVTDPRSD AQAIKEASYV NIPVIALTDL DSPSEFVDVA I PCNNRGKH ...String:
MSLPATFDLT PEDAQLLLAA NTHLGARNVQ VHQEPYVFNA RPDGVHVINV GKTWEKLVLA ARIIAAIPNP EDVVAISSRT FGQRAVLKF AAHTGATPIA GRFTPGSFTN YITRSFKEPR LVIVTDPRSD AQAIKEASYV NIPVIALTDL DSPSEFVDVA I PCNNRGKH SIGLIWYLLA REVLRLRGAL VDRTQPWSIM PDLYFYRDPE EVEQQVAEEA TTEEAGEEEA KEEVTEEQAE AT EWAEENA DNVEW

+
Macromolecule #2: 40S ribosomal protein S1-A

MacromoleculeName: 40S ribosomal protein S1-A / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 28.798467 KDa
SequenceString: MAVGKNKRLS KGKKGQKKRV VDPFTRKEWF DIKAPSTFEN RNVGKTLVNK STGLKSASDA LKGRVVEVCL ADLQGSEDHS FRKIKLRVD EVQGKNLLTN FHGMDFTTDK LRSMVRKWQT LIEANVTVKT SDDYVLRIFA IAFTRKQANQ VKRHSYAQSS H IRAIRKVI ...String:
MAVGKNKRLS KGKKGQKKRV VDPFTRKEWF DIKAPSTFEN RNVGKTLVNK STGLKSASDA LKGRVVEVCL ADLQGSEDHS FRKIKLRVD EVQGKNLLTN FHGMDFTTDK LRSMVRKWQT LIEANVTVKT SDDYVLRIFA IAFTRKQANQ VKRHSYAQSS H IRAIRKVI SEILTKEVQG STLAQLTSKL IPEVINKEIE NATKDIFPLQ NIHVRKVKLL KQPKFDVGAL MALHGEGSGE EK GKKVTGF KDEVLETV

+
Macromolecule #3: 40S ribosomal protein S2

MacromoleculeName: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 27.490826 KDa
SequenceString: MSAPEAQQQK RGGFGGRNRG RPNRRGPRNT EEKGWVPVTK LGRLVKAGKI TTIEEIFLHS LPVKEFQIID TLLPGLQDEV MNIKPVQKQ TRAGQRTRFK AVVVVGDSNG HVGLGIKTAK EVAGAIRAGI IIAKLSVIPI RRGYWGTNLG QPHSLATKTT G KCGSVTVR ...String:
MSAPEAQQQK RGGFGGRNRG RPNRRGPRNT EEKGWVPVTK LGRLVKAGKI TTIEEIFLHS LPVKEFQIID TLLPGLQDEV MNIKPVQKQ TRAGQRTRFK AVVVVGDSNG HVGLGIKTAK EVAGAIRAGI IIAKLSVIPI RRGYWGTNLG QPHSLATKTT G KCGSVTVR LIPAPRGSGI VASPAVKKLL QLAGVEDVYT QSNGKTRTLE NTLKAAFVAI GNTYGFLTPN LWAEQPLPVS PL DIYSDEA SAQKKRF

+
Macromolecule #4: 40S ribosomal protein S4-B

MacromoleculeName: 40S ribosomal protein S4-B / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 29.46933 KDa
SequenceString: MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE VKAILMQRHV KVDGKVRTDT TYPAGFMDV ITLDATNENF RLVYDVKGRF AVHRITDEEA SYKLGKVKKV QLGKKGVPYV VTHDGRTIRY PDPNIKVNDT V KIDLASGK ...String:
MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE VKAILMQRHV KVDGKVRTDT TYPAGFMDV ITLDATNENF RLVYDVKGRF AVHRITDEEA SYKLGKVKKV QLGKKGVPYV VTHDGRTIRY PDPNIKVNDT V KIDLASGK ITDFIKFDAG KLVYVTGGRN LGRIGTIVHK ERHDGGFDLV HIKDSLDNTF VTRLNNVFVI GEQGKPYISL PK GKGIKLS IAEERDRRRA QQGL

+
Macromolecule #5: 40S ribosomal protein S6-B

MacromoleculeName: 40S ribosomal protein S6-B / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 27.054486 KDa
SequenceString: MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRD GERKRKSVRG AIVGPDLAVL ALVIVKKGEQ ELEGLTDTTV PKRLGPKRAN NIRKFFGLSK EDDVRDFVIR R EVTKGEKT ...String:
MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRD GERKRKSVRG AIVGPDLAVL ALVIVKKGEQ ELEGLTDTTV PKRLGPKRAN NIRKFFGLSK EDDVRDFVIR R EVTKGEKT YTKAPKIQRL VTPQRLQRKR HQRALKVRNA QAQREAAAEY AQLLAKRLSE RKAEKAEIRK RRASSLKA

+
Macromolecule #6: 40S ribosomal protein S8-A

MacromoleculeName: 40S ribosomal protein S8-A / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 22.537803 KDa
SequenceString: MGISRDSRHK RSATGAKRAQ FRKKRKFELG RQPANTKIGA KRIHSVRTRG GNKKYRALRI ETGNFSWASE GISKKTRIAG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWFEAHYGQ TLGKKKNVKE EETVAKSKNA ERKWAARAAS AKIESSVESQ F SAGRLYAC ...String:
MGISRDSRHK RSATGAKRAQ FRKKRKFELG RQPANTKIGA KRIHSVRTRG GNKKYRALRI ETGNFSWASE GISKKTRIAG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWFEAHYGQ TLGKKKNVKE EETVAKSKNA ERKWAARAAS AKIESSVESQ F SAGRLYAC ISSRPGQSGR CDGYILEGEE LAFYLRRLTA KK

+
Macromolecule #7: 40S ribosomal protein S9-A

MacromoleculeName: 40S ribosomal protein S9-A / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 22.487893 KDa
SequenceString: MPRAPRTYSK TYSTPKRPYE SSRLDAELKL AGEFGLKNKK EIYRISFQLS KIRRAARDLL TRDEKDPKRL FEGNALIRRL VRVGVLSED KKKLDYVLAL KVEDFLERRL QTQVYKLGLA KSVHHARVLI TQRHIAVGKQ IVNIPSFMVR LDSEKHIDFA P TSPFGGAR ...String:
MPRAPRTYSK TYSTPKRPYE SSRLDAELKL AGEFGLKNKK EIYRISFQLS KIRRAARDLL TRDEKDPKRL FEGNALIRRL VRVGVLSED KKKLDYVLAL KVEDFLERRL QTQVYKLGLA KSVHHARVLI TQRHIAVGKQ IVNIPSFMVR LDSEKHIDFA P TSPFGGAR PGRVARRNAA RKAEASGEAA DEADEADEE

+
Macromolecule #8: 40S ribosomal protein S11-A

MacromoleculeName: 40S ribosomal protein S11-A / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 17.785934 KDa
SequenceString:
MSTELTVQSE RAFQKQPHIF NNPKVKTSKR TKRWYKNAGL GFKTPKTAIE GSYIDKKCPF TGLVSIRGKI LTGTVVSTKM HRTIVIRRA YLHYIPKYNR YEKRHKNVPV HVSPAFRVQV GDIVTVGQCR PISKTVRFNV VKVSAAAGKA NKQFAKF

+
Macromolecule #9: 40S ribosomal protein S13

MacromoleculeName: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 17.059945 KDa
SequenceString:
MGRMHSAGKG ISSSAIPYSR NAPAWFKLSS ESVIEQIVKY ARKGLTPSQI GVLLRDAHGV TQARVITGNK IMRILKSNGL APEIPEDLY YLIKKAVSVR KHLERNRKDK DAKFRLILIE SRIHRLARYY RTVAVLPPNW KYESATASAL VN

+
Macromolecule #10: 40S ribosomal protein S14-A

MacromoleculeName: 40S ribosomal protein S14-A / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 14.562655 KDa
SequenceString:
MSNVVQARDN SQVFGVARIY ASFNDTFVHV TDLSGKETIA RVTGGMKVKA DRDESSPYAA MLAAQDVAAK CKEVGITAVH VKIRATGGT RTKTPGPGGQ AALRALARSG LRIGRIEDVT PVPSDSTRKK GGRRGRRL

+
Macromolecule #11: 40S ribosomal protein S21-A

MacromoleculeName: 40S ribosomal protein S21-A / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 9.758829 KDa
SequenceString:
MENDKGQLVE LYVPRKCSAT NRIIKADDHA SVQINVAKVD EEGRAIPGEY VTYALSGYVR SRGESDDSLN RLAQNDGLLK NVWSYSR

+
Macromolecule #12: 40S ribosomal protein S22-A

MacromoleculeName: 40S ribosomal protein S22-A / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 14.650062 KDa
SequenceString:
MTRSSVLADA LNAINNAEKT GKRQVLIRPS SKVIIKFLQV MQKHGYIGEF EYIDDHRSGK IVVQLNGRLN KCGVISPRFN VKIGDIEKW TANLLPARQF GYVILTTSAG IMDHEEARRK HVSGKILGFV Y

+
Macromolecule #13: 40S ribosomal protein S23-A

MacromoleculeName: 40S ribosomal protein S23-A / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 16.073896 KDa
SequenceString:
MGKGKPRGLN SARKLRVHRR NNRWAENNYK KRLLGTAFKS SPFGGSSHAK GIVLEKLGIE SKQPNSAIRK CVRVQLIKNG KKVTAFVPN DGCLNFVDEN DEVLLAGFGR KGKAKGDIPG VRFKVVKVSG VSLLALWKEK KEKPRS

+
Macromolecule #14: 40S ribosomal protein S24-A

MacromoleculeName: 40S ribosomal protein S24-A / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 15.362848 KDa
SequenceString:
MSDAVTIRTR KVISNPLLAR KQFVVDVLHP NRANVSKDEL REKLAEVYKA EKDAVSVFGF RTQFGGGKSV GFGLVYNSVA EAKKFEPTY RLVRYGLAEK VEKASRQQRK QKKNRDKKIF GTGKRLAKKV ARRNAD

+
Macromolecule #15: 40S ribosomal protein S26-A

MacromoleculeName: 40S ribosomal protein S26-A / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 13.538922 KDa
SequenceString:
MPKKRASNGR NKKGRGHVKP VRCVNCSKSI PKDKAIKRMA IRNIVEAAAV RDLSEASVYP EYALPKTYNK LHYCVSCAIH ARIVRVRSR EDRKNRAPPQ RPRFNRENKV SPADAAKKAL

+
Macromolecule #16: 40S ribosomal protein S27-A

MacromoleculeName: 40S ribosomal protein S27-A / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 8.893391 KDa
SequenceString:
MVLVQDLLHP TAASEARKHK LKTLVQGPRS YFLDVKCPGC LNITTVFSHA QTAVTCESCS TILCTPTGGK AKLSEGTSFR RK

+
Macromolecule #17: 40S ribosomal protein S30-A

MacromoleculeName: 40S ribosomal protein S30-A / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 7.137541 KDa
SequenceString:
MAKVHGSLAR AGKVKSQTPK VEKTEKPKKP KGRAYKRLLY TRRFVNVTLV NGKRRMNPGP SVQ

+
Macromolecule #18: RPS15 isoform 1

MacromoleculeName: RPS15 isoform 1 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 16.031907 KDa
SequenceString:
MSQAVNAKKR VFKTHSYRGV DLEKLLEMST EDFVKLAPAR VRRRFARGMT SKPAGFMKKL RAAKLAAPEN EKPAPVRTHM RNMIIVPEM IGSVVGIYNG KAFNQVEIRP EMLGHYLGEF SITYTPVRHG RAGATTSRFI PLK

+
Macromolecule #19: 40S ribosomal protein S3

MacromoleculeName: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 26.542789 KDa
SequenceString: MVALISKKRK LVADGVFYAE LNEFFTRELA EEGYSGVEVR VTPTKTEVII RATRTQDVLG ENGRRINELT LLVQKRFKYA PGTIVLYAE RVQDRGLSAV AQAESMKFKL LNGLAIRRAA YGVVRYVMES GAKGCEVVVS GKLRAARAKA MKFADGFLIH S GQPVNDFI ...String:
MVALISKKRK LVADGVFYAE LNEFFTRELA EEGYSGVEVR VTPTKTEVII RATRTQDVLG ENGRRINELT LLVQKRFKYA PGTIVLYAE RVQDRGLSAV AQAESMKFKL LNGLAIRRAA YGVVRYVMES GAKGCEVVVS GKLRAARAKA MKFADGFLIH S GQPVNDFI DTATRHVLMR QGVLGIKVKI MRDPAKSRTG PKALPDAVTI IEPKEEEPIL APSVKDYRPA EETEAQAEPV EA

+
Macromolecule #20: 40S ribosomal protein S10-A

MacromoleculeName: 40S ribosomal protein S10-A / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 12.757445 KDa
SequenceString:
MLMPKEDRNK IHQYLFQEGV VVAKKDFNQA KHEEIDTKNL YVIKALQSLT SKGYVKTQFS WQYYYYTLTE EGVEYLREYL NLPEHIVPG TYIQERNPTQ RPQRRY

+
Macromolecule #21: 40S ribosomal protein S12

MacromoleculeName: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 15.488631 KDa
SequenceString:
MSDVEEVVEV QEETVVEQTA EVTIEDALKV VLRTALVHDG LARGLRESTK ALTRGEALLV VLVSSVTEAN IIKLVEGLAN DPENKVPLI KVADAKQLGE WAGLGKIDRE GNARKVVGAS VVVVKNWGAE TDELSMIMEH FSQQ

+
Macromolecule #22: 40S ribosomal protein S16-A

MacromoleculeName: 40S ribosomal protein S16-A / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 15.87749 KDa
SequenceString:
MSAVPSVQTF GKKKSATAVA HVKAGKGLIK VNGSPITLVE PEILRFKVYE PLLLVGLDKF SNIDIRVRVT GGGHVSQVYA IRQAIAKGL VAYHQKYVDE QSKNELKKAF TSYDRTLLIA DSRRPEPKKF GGKGARSRFQ KSYR

+
Macromolecule #23: 40S ribosomal protein S17-A

MacromoleculeName: 40S ribosomal protein S17-A / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 15.820413 KDa
SequenceString:
MGRVRTKTVK RASKALIERY YPKLTLDFQT NKRLCDEIAT IQSKRLRNKI AGYTTHLMKR IQKGPVRGIS FKLQEEERER KDQYVPEVS ALDLSRSNGV LNVDNQTSDL VKSLGLKLPL SVINVSAQRD RRYRKRV

+
Macromolecule #24: 40S ribosomal protein S18-A

MacromoleculeName: 40S ribosomal protein S18-A / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 17.071641 KDa
SequenceString:
MSLVVQEQGS FQHILRLLNT NVDGNIKIVY ALTTIKGVGR RYSNLVCKKA DVDLHKRAGE LTQEELERIV QIMQNPTHYK IPAWFLNRQ NDITDGKDYH TLANNVESKL RDDLERLKKI RAHRGIRHFW GLRVRGQHTK TTGRRRA

+
Macromolecule #25: 40S ribosomal protein S19-A

MacromoleculeName: 40S ribosomal protein S19-A / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 15.942125 KDa
SequenceString:
MPGVSVRDVA AQDFINAYAS FLQRQGKLEV PGYVDIVKTS SGNEMPPQDA EGWFYKRAAS VARHIYMRKQ VGVGKLNKLY GGAKSRGVR PYKHIDASGS INRKVLQALE KIGIVEISPK GGRRISENGQ RDLDRIAAQT LEEDE

+
Macromolecule #26: 40S ribosomal protein S20

MacromoleculeName: 40S ribosomal protein S20 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 13.929044 KDa
SequenceString:
MSDFQKEKVE EQEQQQQQII KIRITLTSTK VKQLENVSSN IVKNAEQHNL VKKGPVRLPT KVLKISTRKT PNGEGSKTWE TYEMRIHKR YIDLEAPVQI VKRITQITIE PGVDVEVVVA SN

+
Macromolecule #27: 40S ribosomal protein S25-A

MacromoleculeName: 40S ribosomal protein S25-A / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 12.067272 KDa
SequenceString:
MPPKQQLSKA AKAAAALAGG KKSKKKWSKK SMKDRAQHAV ILDQEKYDRI LKEVPTYRYV SVSVLVDRLK IGGSLARIAL RHLEKEGII KPISKHSKQA IYTRATASE

+
Macromolecule #28: RPS29A isoform 1

MacromoleculeName: RPS29A isoform 1 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 6.675723 KDa
SequenceString:
MAHENVWFSH PRRYGKGSRQ CRVCSSHTGL IRKYGLNICR QCFREKANDI GFNKFR

+
Macromolecule #29: 40S ribosomal protein S31

MacromoleculeName: 40S ribosomal protein S31 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 8.703462 KDa
SequenceString:
GKKRKKKVYT TPKKIKHKHK KVKLAVLSYY KVDAEGKVTK LRRECSNPTC GAGVFLANHK DRLYCGKCHS VYKVNA

+
Macromolecule #30: Guanine nucleotide-binding protein subunit beta-like protein

MacromoleculeName: Guanine nucleotide-binding protein subunit beta-like protein
type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 34.841219 KDa
SequenceString: MASNEVLVLR GTLEGHNGWV TSLATSAGQP NLLLSASRDK TLISWKLTGD DQKFGVPVRS FKGHSHIVQD CTLTADGAYA LSASWDKTL RLWDVATGET YQRFVGHKSD VMSVDIDKKA SMIISGSRDK TIKVWTIKGQ CLATLLGHND WVSQVRVVPN E KADDDSVT ...String:
MASNEVLVLR GTLEGHNGWV TSLATSAGQP NLLLSASRDK TLISWKLTGD DQKFGVPVRS FKGHSHIVQD CTLTADGAYA LSASWDKTL RLWDVATGET YQRFVGHKSD VMSVDIDKKA SMIISGSRDK TIKVWTIKGQ CLATLLGHND WVSQVRVVPN E KADDDSVT IISAGNDKMV KAWNLNQFQI EADFIGHNSN INTLTASPDG TLIASAGKDG EIMLWNLAAK KAMYTLSAQD EV FSLAFSP NRYWLAAATA TGIKVFSLDP QYLVDDLRPE FAGYSKAAEP HAVSLAWSAD GQTLFAGYTD NVIRVWQVMT AN

+
Macromolecule #31: 40S ribosomal protein S28-A

MacromoleculeName: 40S ribosomal protein S28-A / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 7.605847 KDa
SequenceString:
MDNKTPVTLA KVIKVLGRTG SRGGVTQVRV EFLEDTSRTI VRNVKGPVRE NDILVLMESE REARRLR

+
Macromolecule #32: Eukaryotic translation initiation factor 3 subunit I

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit I / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 38.803375 KDa
SequenceString: MKAIKLTGHE RPLTQVKYNK EGDLLFSCSK DSSASVWYSL NGERLGTLDG HTGTIWSIDV DCFTKYCVTG SADYSIKLWD VSNGQCVAT WKSPVPVKRV EFSPCGNYFL AILDNVMKNP GSINIYEIER DSATHELTKV SEEPIHKIIT HEGLDAATVA G WSTKGKYI ...String:
MKAIKLTGHE RPLTQVKYNK EGDLLFSCSK DSSASVWYSL NGERLGTLDG HTGTIWSIDV DCFTKYCVTG SADYSIKLWD VSNGQCVAT WKSPVPVKRV EFSPCGNYFL AILDNVMKNP GSINIYEIER DSATHELTKV SEEPIHKIIT HEGLDAATVA G WSTKGKYI IAGHKDGKIS KYDVSNNYEY VDSIDLHEKS ISDMQFSPDL TYFITSSRDT NSFLVDVSTL QVLKKYETDC PL NTAVITP LKEFIILGGG QEAKDVTTTS ANEGKFEARF YHKIFEEEIG RVQGHFGPLN TVAISPQGTS YASGGEDGFI RLH HFEKSY FDFKYDVEKA AEAKEHMQEA N

+
Macromolecule #33: Eukaryotic translation initiation factor 3 subunit G

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit G / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 30.547525 KDa
SequenceString: MSEVAPEEII ENADGSRSII TYKIEDGVKY KITQKVKEVK VLEKVHKSVA ERKNWHKYGS EKGSPAGPSA VTARLGEEVE LRLSRNWKQ AEEERIQKEK ASLTKTGLQC RLCGNDHMTM NCPFKTILSE LSALEDPATN EGGVEAASEE KAGQVGGAGS I PGQYVPPS ...String:
MSEVAPEEII ENADGSRSII TYKIEDGVKY KITQKVKEVK VLEKVHKSVA ERKNWHKYGS EKGSPAGPSA VTARLGEEVE LRLSRNWKQ AEEERIQKEK ASLTKTGLQC RLCGNDHMTM NCPFKTILSE LSALEDPATN EGGVEAASEE KAGQVGGAGS I PGQYVPPS RRAGARDPSS DAYRDSRERD DMCTLKIMQV NENADENSLR EELLFPFAPI PRVSVVRNKE TGKSRGLAFV TF SSEEVAE QALRFLDGRG YMNLILRVEW SKPKVKE

+
Macromolecule #35: TIF5 isoform 1

MacromoleculeName: TIF5 isoform 1 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 45.321977 KDa
SequenceString: MSINICRDNH DPFYRYKMPP IQAKVEGRGN GIKTAVLNVA DISHALNRPA PYIVKYFGFE LGAQTSISVD KDRYLVNGVH EPAKLQDVL DGFINKFVLC GSCKNPETEI IITKDNDLVR DCKACGKRTP MDLRHKLSSF ILKNPPDSVS GSKKKKKAAT A SANVRGGG ...String:
MSINICRDNH DPFYRYKMPP IQAKVEGRGN GIKTAVLNVA DISHALNRPA PYIVKYFGFE LGAQTSISVD KDRYLVNGVH EPAKLQDVL DGFINKFVLC GSCKNPETEI IITKDNDLVR DCKACGKRTP MDLRHKLSSF ILKNPPDSVS GSKKKKKAAT A SANVRGGG LSISDIAQGK SQNAPSDGTG SSTPQHHDED EDELSRQIKA AASTLEDIEV KDDEWAVDMS EEAIRARAKE LE VNSELTQ LDEYGEWILE QAGEDKENLP SDVELYKKAA ELDVLNDPKI GCVLAQCLFD EDIVNEIAEH NAFFTKILVT PEY EKNFMG GIERFLGLEH KDLIPLLPKI LVQLYNNDII SEEEIMRFGT KSSKKFVPKE VSKKVRRAAK PFITWLETAE SDDD EEDDE

+
Macromolecule #36: Eukaryotic translation initiation factor 3 subunit A

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit A / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 110.517641 KDa
SequenceString: MAPPPFRPEN AIKRADELIS VGEKQAALQS LHDFITARRI RWATPSTVEP VVFKFLEIGV ELKKGKLLKD GLHQYKKLIQ GSTEGLVSV GAVARKFIDL VESKIASEQT RADELQKQEI DDDLEGGVTP ENLLISVYES DQSVAGFNDE AITSWLRFTW E SYRAVLDL ...String:
MAPPPFRPEN AIKRADELIS VGEKQAALQS LHDFITARRI RWATPSTVEP VVFKFLEIGV ELKKGKLLKD GLHQYKKLIQ GSTEGLVSV GAVARKFIDL VESKIASEQT RADELQKQEI DDDLEGGVTP ENLLISVYES DQSVAGFNDE AITSWLRFTW E SYRAVLDL LRNNALLEIT YSGVVKKTMH FCLKYQRKNE FKRLAEMLRQ HLDAANYQQS KSGNNLVDLS DADTLQRYLD QR FQQVDVS VKLELWHEAY RSIEDVFHLM KISKRAPKPS TLANYYENLV KVFFVSGDPL LHTTAWKKFY KLYSTNPRAT EEE FKTYSS TIFLSAISTQ LDEIPSIGYD PHLRMYRLLN LDAKPTRKEM LQSIIEDESI YGKVDEELKE LYDIIEVNFD VDTV KQQLE NLLVKLSSKT YFSQYIAPLR DVIMRRVFVA ASQKFTTVSQ SELYKLATLP APLDLSAWDI EKSLLQAAVE DYVSI TIDH ESAKVTFAKD PFDIFASTAS KEVSEEENTE PEVQEEKEET DEALGPQETE DGEEKEEESD PVIIRNSYIH NKLLEL SNV LHDVDSFNNA SYMEKVRIAR ETLIKKNKDD LEKISKIVDE RVKRSQEQKQ KHMEHAALHA EQDAEVRQQR ILEEKAA IE AKLEEEAHRR LIEKKKREFE AIKEREITKM ITEVNAKGHV YIDPNEAKSL DLDTIKQVII AEVSKNKSEL ESRMEYAM K KLDHTERALR KVELPLLQKE VDKLQETDTA NYEAMKKKIV DAAKAEYEAR MADRKNLVMV YDDYLKFKEH VSGTKESEL AAIRNQKKAE LEAAKKARIE EVRKRRYEEA IARRKEEIAN AERQKRAQEL AEATRKQREI EEAAAKKSTP YSFRAGNREP PSTPSTLPK ATVSPDKAKL DMIAQKQREM EEAIEQRLAG RTAGGSSPAT PATPATPATP TPSSGPKKMT MAEKLRAKRL A KGGR

+
Macromolecule #37: Eukaryotic translation initiation factor 3 subunit B

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit B / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 88.241766 KDa
SequenceString: MKNFLPRTLK NIYELYFNNI SVHSIVSRNT QLKRSKIIQM TTETFEDIKL EDIPVDDIDF SDLEEQYKVT EEFNFDQYIV VNGAPVIPS AKVPVLKKAL TSLFSKAGKV VNMEFPIDEA TGKTKGFLFV ECGSMNDAKK IIKSFHGKRL DLKHRLFLYT M KDVERYNS ...String:
MKNFLPRTLK NIYELYFNNI SVHSIVSRNT QLKRSKIIQM TTETFEDIKL EDIPVDDIDF SDLEEQYKVT EEFNFDQYIV VNGAPVIPS AKVPVLKKAL TSLFSKAGKV VNMEFPIDEA TGKTKGFLFV ECGSMNDAKK IIKSFHGKRL DLKHRLFLYT M KDVERYNS DDFDTEFREP DMPTFVPSSS LKSWLMDDKV RDQFVLQDDV KTSVFWNSMF NEEDSLVESR ENWSTNYVRF SP KGTYLFS YHQQGVTAWG GPNFDRLRRF YHPDVRNSSV SPNEKYLVTF STEPIIVEED NEFSPFTKKN EGHQLCIWDI ASG LLMATF PVIKSPYLKW PLVRWSYNDK YCARMVGDSL IVHDATKNFM PLEAKALKPS GIRDFSFAPE GVKLQPFRNG DEPS VLLAY WTPETNNSAC TATIAEVPRG RVLKTVNLVQ VSNVTLHWQN QAEFLCFNVE RHTKSGKTQF SNLQICRLTE RDIPV EKVE LKDSVFEFGW EPHGNRFVTI SVHEVADMNY AIPANTIRFY APETKEKTDV IKRWSLVKEI PKTFANTVSW SPAGRF VVV GALVGPNMRR SDLQFYDMDY PGEKNINDNN DVSASLKDVA HPTYSAATNI TWDPSGRYVT AWSSSLKHKV EHGYKIF NI AGNLVKEDII AGFKNFAWRP RPASILSNAE RKKVRKNLRE WSAQFEEQDA MEADTAMRDL ILHQRELLKQ WTEYREKI G QEMEKSMNFK IFDVQPEDAS DDFTTIEEIV EEVLEETKEK VE

+
Macromolecule #38: Eukaryotic translation initiation factor 3 subunit C

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit C / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 93.310172 KDa
SequenceString: MSRFFSSNYE YDVASSSSEE DLLSSSEEDL LSSSSSESEL DQESDDSFFN ESESESEADV DSDDSDAKPY GPDWFKKSEF RKQGGGSNK FLKSSNYDSS DEESDEEDGK KVVKSAKEKL LDEMQDVYNK ISQAENSDDW LTISNEFDLI SRLLVRAQQQ N WGTPNIFI ...String:
MSRFFSSNYE YDVASSSSEE DLLSSSEEDL LSSSSSESEL DQESDDSFFN ESESESEADV DSDDSDAKPY GPDWFKKSEF RKQGGGSNK FLKSSNYDSS DEESDEEDGK KVVKSAKEKL LDEMQDVYNK ISQAENSDDW LTISNEFDLI SRLLVRAQQQ N WGTPNIFI KVVAQVEDAV NNTQQADLKN KAVARAYNTT KQRVKKVSRE NEDSMAKFRN DPESFDKEPT ADLDISANGF TI SSSQGND QAVQEDFFTR LQTIIDSRGK KTVNQQSLIS TLEELLTVAE KPYEFIMAYL TLIPSRFDAS ANLSYQPIDQ WKS SFNDIS KLLSILDQTI DTYQVNEFAD PIDFIEDEPK EDSDGVKRIL GSIFSFVERL DDEFMKSLLN IDPHSSDYLI RLRD EQSIY NLILRTQLYF EATLKDEHDL ERALTRPFVK RLDHIYYKSE NLIKIMETAA WNIIPAQFKS KFTSKDQLDS ADYVD NLID GLSTILSKQN NIAVQKRAIL YNIYYTALNK DFQTAKDMLL TSQVQTNINQ FDSSLQILFN RVVVQLGLSA FKLCLI EEC HQILNDLLSS SHLREILGQQ SLHRISLNSS NNASADERAR QCLPYHQHIN LDLIDVVFLT CSLLIEIPRM TAFYSGI KV KRIPYSPKSI RRSLEHYDKL SFQGPPETLR DYVLFAAKSM QKGNWRDSVK YLREIKSWAL LPNMETVLNS LTERVQVE S LKTYFFSFKR FYSSFSVAKL AELFDLPENK VVEVLQSVIA ELEIPAKLND EKTIFVVEKG DEITKLEEAM VKLNKEYKI AKERLNPPSN RR

+
Macromolecule #40: Eukaryotic translation initiation factor 4C

MacromoleculeName: Eukaryotic translation initiation factor 4C / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 17.462168 KDa
SequenceString:
MGKKNTKGGK KGRRGKNDSD GPKRELIYKE EGQEYAQITK MLGNGRVEAS CFDGNKRMAH IRGKLRKKVW MGQGDIILVS LRDFQDDQC DVVHKYNLDE ARTLKNQGEL PENAKINETD NFGFESDEDV NFEFGNADED DEEGEDEELD IDDI

+
Macromolecule #41: RLI1 isoform 1

MacromoleculeName: RLI1 isoform 1 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 68.433242 KDa
SequenceString: MSDKNSRIAI VSADKCKPKK CRQECKRSCP VVKTGKLCIE VTPTSKIAFI SEILCIGCGI CVKKCPFDAI QIINLPTNLE AHVTHRYSA NSFKLHRLPT PRPGQVLGLV GTNGIGKSTA LKILAGKQKP NLGRFDDPPE WQEIIKYFRG SELQNYFTKM L EDDIKAII ...String:
MSDKNSRIAI VSADKCKPKK CRQECKRSCP VVKTGKLCIE VTPTSKIAFI SEILCIGCGI CVKKCPFDAI QIINLPTNLE AHVTHRYSA NSFKLHRLPT PRPGQVLGLV GTNGIGKSTA LKILAGKQKP NLGRFDDPPE WQEIIKYFRG SELQNYFTKM L EDDIKAII KPQYVDNIPR AIKGPVQKVG ELLKLRMEKS PEDVKRYIKI LQLENVLKRD IEKLSGGELQ RFAIGMSCVQ EA DVYMFDE PSSYLDVKQR LNAAQIIRSL LAPTKYVICV EHDLSVLDYL SDFVCIIYGV PSVYGVVTLP ASVREGINIF LDG HIPAEN LRFRTEALQF RIADATEDLQ NDSASRAFSY PSLKKTQGDF VLNVEEGEFS DSEILVMMGE NGTGKTTLIK LLAG ALKPD EGQDIPKLNV SMKPQKIAPK FPGTVRQLFF KKIRGQFLNP QFQTDVVKPL RIDDIIDQEV QHLSGGELQR VAIVL ALGI PADIYLIDEP SAYLDSEQRI ICSKVIRRFI LHNKKTAFIV EHDFIMATYL ADKVIVFEGI PSKNAHARAP ESLLTG CNR FLKNLNVTFR RDPNSFRPRI NKLDSQMDKE QKSSGNYFFL DNTGI

+
Macromolecule #43: 40S ribosomal protein S5

MacromoleculeName: 40S ribosomal protein S5 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 25.0726 KDa
SequenceString: MSDTEAPVEV QEDFEVVEEF TPVVLATPIP EEVQQAQTEI KLFNKWSFEE VEVKDASLVD YVQVRQPIFV AHTAGRYANK RFRKAQCPI IERLTNSLMM NGRNNGKKLK AVRIIKHTLD IINVLTDQNP IQVVVDAITN TGPREDTTRV GGGGAARRQA V DVSPLRRV ...String:
MSDTEAPVEV QEDFEVVEEF TPVVLATPIP EEVQQAQTEI KLFNKWSFEE VEVKDASLVD YVQVRQPIFV AHTAGRYANK RFRKAQCPI IERLTNSLMM NGRNNGKKLK AVRIIKHTLD IINVLTDQNP IQVVVDAITN TGPREDTTRV GGGGAARRQA V DVSPLRRV NQAIALLTIG AREAAFRNIK TIAETLAEEL INAAKGSSTS YAIKKKDELE RVAKSNR

+
Macromolecule #44: 60S ribosomal protein L40-A

MacromoleculeName: 60S ribosomal protein L40-A / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 8.576831 KDa
SequenceString:
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

+
Macromolecule #45: 60S ribosomal protein L41-A

MacromoleculeName: 60S ribosomal protein L41-A / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 3.354243 KDa
SequenceString:
MRAKWRKKRT RRLKRKRRKV RARSK

+
Macromolecule #46: SUI2 isoform 1

MacromoleculeName: SUI2 isoform 1 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 34.763652 KDa
SequenceString: MSTSHCRFYE NKYPEIDDIV MVNVQQIAEM GAYVKLLEYD NIEGMILLSE LSRRRIRSIQ KLIRVGKNDV AVVLRVDKEK GYIDLSKRR VSSEDIIKCE EKYQKSKTVH SILRYCAEKF QIPLEELYKT IAWPLSRKFG HAYEAFKLSI IDETVWEGIE P PSKDVLDE ...String:
MSTSHCRFYE NKYPEIDDIV MVNVQQIAEM GAYVKLLEYD NIEGMILLSE LSRRRIRSIQ KLIRVGKNDV AVVLRVDKEK GYIDLSKRR VSSEDIIKCE EKYQKSKTVH SILRYCAEKF QIPLEELYKT IAWPLSRKFG HAYEAFKLSI IDETVWEGIE P PSKDVLDE LKNYISKRLT PQAVKIRADV EVSCFSYEGI DAIKDALKSA EDMSTEQMQV KVKLVAAPLY VLTTQALDKQ KG IEQLESA IEKITEVITK YGGVCNITMP PKAVTATEDA ELQALLESKE LDNRSDSEDD EDESDDE

+
Macromolecule #47: protein-synthesizing GTPase

MacromoleculeName: protein-synthesizing GTPase / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO / EC number: protein-synthesizing GTPase
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 57.942699 KDa
SequenceString: MSDLQDQEPS IIINGNLEPV GEPDIVEETE VVAQETQETQ DADKPKKKVA FTGLEEDGET EEEKRKREFE EGGGLPEQPL NPDFSKLNP LSAEIINRQA TINIGTIGHV AHGKSTVVRA ISGVQTVRFK DELERNITIK LGYANAKIYK CQEPTCPEPD C YRSFKSDK ...String:
MSDLQDQEPS IIINGNLEPV GEPDIVEETE VVAQETQETQ DADKPKKKVA FTGLEEDGET EEEKRKREFE EGGGLPEQPL NPDFSKLNP LSAEIINRQA TINIGTIGHV AHGKSTVVRA ISGVQTVRFK DELERNITIK LGYANAKIYK CQEPTCPEPD C YRSFKSDK EISPKCQRPG CPGRYKLVRH VSFVDCPGHD ILMSTMLSGA AVMDAALLLI AGNESCPQPQ TSEHLAAIEI MK LKHVIIL QNKVDLMREE SALEHQKSIL KFIRGTIADG APIVPISAQL KYNIDAVNEF IVKTIPVPPR DFMISPRLIV IRS FDVNKP GAEIEDLKGG VAGGSILNGV FKLGDEIEIR PGIVTKDDKG KIQCKPIFSN IVSLFAEQND LKFAVPGGLI GVGT KVDPT LCRADRLVGQ VVGAKGHLPN IYTDIEINYF LLRRLLGVKT DGQKQAKVRK LEPNEVLMVN IGSTATGARV VAVKA DMAR LQLTSPACTE INEKIALSRR IEKHWRLIGW ATIKKGTTLE PIA

+
Macromolecule #48: SUI3 isoform 1

MacromoleculeName: SUI3 isoform 1 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 31.631309 KDa
SequenceString: MSSDLAAELG FDPALKKKKK TKKVIPDDFD AAVNGKENGS GDDLFAGLKK KKKKSKSVSA DAEAEKEPTD DIAEALGELS LKKKKKKTK DSSVDAFEKE LAKAGLDNVD AESKEGTPSA NSSIQQEVGL PYSELLSRFF NILRTNNPEL AGDRSGPKFR I PPPVCLRD ...String:
MSSDLAAELG FDPALKKKKK TKKVIPDDFD AAVNGKENGS GDDLFAGLKK KKKKSKSVSA DAEAEKEPTD DIAEALGELS LKKKKKKTK DSSVDAFEKE LAKAGLDNVD AESKEGTPSA NSSIQQEVGL PYSELLSRFF NILRTNNPEL AGDRSGPKFR I PPPVCLRD GKKTIFSNIQ DIAEKLHRSP EHLIQYLFAE LGTSGSVDGQ KRLVIKGKFQ SKQMENVLRR YILEYVTCKT CK SINTELK REQSNRLFFM VCKSCGSTRS VSSIKTGFQA TVGKRRRM

+
Macromolecule #49: OTU domain-containing protein 2

MacromoleculeName: OTU domain-containing protein 2 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 36.157016 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae W303 (yeast)
SequenceString: MTGMESGENL ENMEDILARH RKENKDLQNK ITGMKKQATK SKRKEVNSKC LDLQDKLKTK QENEIRDWKI ANNEVFDAEQ EDEVTPEKL LEQLSISRDE KEQQNVPVQQ QQQGQTKKRR NRQKERLAKR DAAIAKMKEE AALEASKQPD LKKMEQESID Q LCELKKLK ...String:
MTGMESGENL ENMEDILARH RKENKDLQNK ITGMKKQATK SKRKEVNSKC LDLQDKLKTK QENEIRDWKI ANNEVFDAEQ EDEVTPEKL LEQLSISRDE KEQQNVPVQQ QQQGQTKKRR NRQKERLAKR DAAIAKMKEE AALEASKQPD LKKMEQESID Q LCELKKLK QFDIQPDGHS LFASILDQLK LRHDPKKLDQ DMDVMKLRWL SCNYVQEHRD DFIPYLFDEE TMKMKDIDEY TK EMEHTAQ WGGEIEILAL SHVFDCPISI LMSGRPIQVY NECGKNPELK LVYYKHSYAL GEHYNSLHDS

+
Macromolecule #50: 40S ribosomal protein S7-A

MacromoleculeName: 40S ribosomal protein S7-A / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 21.658209 KDa
SequenceString:
MSAPQAKILS QAPTELELQV AQAFVELENS SPELKAELRP LQFKSIREID VAGGKKALAI FVPVPSLAGF HKVQTKLTRE LEKKFQDRH VIFLAERRIL PKPSRTSRQV QKRPRSRTLT AVHDKILEDL VFPTEIVGKR VRYLVGGNKI QKVLLDSKDV Q QIDYKLES FQAVYNKLTG KQIVFEIPSE TH

+
Macromolecule #34: tRNA

MacromoleculeName: tRNA / type: rna / ID: 34 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 24.712961 KDa
SequenceString:
AGCGCCGU(1MG)(2MG) CGCAG(H2U)GGAA GCGC(M2G)CAGGG CUCAU(T6A)ACCC UGAU(G7M)(H2U)(5MC) (5MC)U CGGAUCG(1MA)AA CCG(RIA)GCGGCG CUACCA

+
Macromolecule #39: mRNA

MacromoleculeName: mRNA / type: rna / ID: 39 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 2.534585 KDa
SequenceString:
AAAAUGUC

+
Macromolecule #42: 18S ribosomal RNA

MacromoleculeName: 18S ribosomal RNA / type: rna / ID: 42 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae W303 (yeast)
Molecular weightTheoretical: 579.761938 KDa
SequenceString: UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGUAUAAC UGUGGUAAUU C UAGAGCUA ...String:
UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGUAUAAC UGUGGUAAUU C UAGAGCUA AUACAUGCUU AAAAUCUCGA CCCUUUGGAA GAGAUGUAUU UAUUAGAUAA AAAAUCAAUG UCUUCGGACU CU UUGAUGA UUCAUAAUAA CUUUUCGAAU CGCAUGGCCU UGUGCUGGCG AUGGUUCAUU CAAAUUUCUG CCCUAUCAAC UUU CGAUGG UAGGAUAGUG GCCUACCAUG GUUUCAACGG GUAACGGGGA AUAAGGGUUC GAUUCCGGAG AGGGAGCCUG AGAA ACGGC UACCACAUCC AAGGAAGGCA GCAGGCGCGC AAAUUACCCA AUCCUAAUUC AGGGAGGUAG UGACAAUAAA UAACG AUAC AGGGCCCAUU CGGGUCUUGU AAUUGGAAUG AGUACAAUGU AAAUACCUUA ACGAGGAACA AUUGGAGGGC AAGUCU GGU GCCAGCAGCC GCGGUAAUUC CAGCUCCAAU AGCGUAUAUU AAAGUUGUUG CAGUUAAAAA GCUCGUAGUU GAACUUU GG GCCCGGUUGG CCGGUCCGAU UUUUUCGUGU ACUGGAUUUC CAACGGGGCC UUUCCUUCUG GCUAACCUUG AGUCCUUG U GGCUCUUGGC GAACCAGGAC UUUUACUUUG AAAAAAUUAG AGUGUUCAAA GCAGGCGUAU UGCUCGAAUA UAUUAGCAU GGAAUAAUAG AAUAGGACGU UUGGUUCUAU UUUGUUGGUU UCUAGGACCA UCGUAAUGAU UAAUAGGGAC GGUCGGGGGC AUCAGUAUU CAAUUGUCAG AGGUGAAAUU CUUGGAUUUA UUGAAGACUA ACUACUGCGA AAGCAUUUGC CAAGGACGUU U UCAUUAAU CAAGAACGAA AGUUAGGGGA UCGAAGAUGA UCAGAUACCG UCGUAGUCUU AACCAUAAAC UAUGCCGACU AG GGAUCGG GUGGUGUUUU UUUAAUGACC CACUCGGCAC CUUACGAGAA AUCAAAGUCU UUGGGUUCUG GGGGGAGUAU GGU CGCAAG GCUGAAACUU AAAGGAAUUG ACGGAAGGGC ACCACCAGGA GUGGAGCCUG CGGCUUAAUU UGACUCAACA CGGG GAAAC UCACCAGGUC CAGACACAAU AAGGAUUGAC AGAUUGAGAG CUCUUUCUUG AUUUUGUGGG UGGUGGUGCA UGGCC GUUC UUAGUUGGUG GAGUGAUUUG UCUGCUUAAU UGCGAUAACG AACGAGACCU UAACCUACUA AAUAGUGGUG CUAGCA UUU GCUGGUUAUC CACUUCUUAG AGGGACUAUC GGUUUCAAGC CGAUGGAAGU UUGAGGCAAU AACAGGUCUG UGAUGCC CU UAGACGUUCU GGGCCGCACG CGCGCUACAC UGACGGAGCC AGCGAGUCUA ACCUUGGCCG AGAGGUCUUG GUAAUCUU G UGAAACUCCG UCGUGCUGGG GAUAGAGCAU UGUAAUUAUU GCUCUUCAAC GAGGAAUUCC UAGUAAGCGC AAGUCAUCA GCUUGCGUUG AUUACGUCCC UGCCCUUUGU ACACACCGCC CGUCGCUAGU ACCGAUUGAA UGGCUUAGUG AGGCCUCAGG AUCUGCUUA GAGAAGGGGG CAACUCCAUC UCAGAGCGGA GAAUUUGGAC AAACUUGGUC AUUUAGAGGA ACUAAAAGUC G UAACAAGG UUUCCGUAGG UGAACCUGCG GAAGGAUCAU UA

+
Macromolecule #51: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 51 / Number of copies: 4 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #52: ADENOSINE-5'-DIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 52 / Number of copies: 1 / Formula: ADP
Molecular weightTheoretical: 427.201 Da
Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

+
Macromolecule #53: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 53 / Number of copies: 5 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #54: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 54 / Number of copies: 1 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

+
Macromolecule #55: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 55 / Number of copies: 2 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

+
Macromolecule #56: METHIONINE

MacromoleculeName: METHIONINE / type: ligand / ID: 56 / Number of copies: 1 / Formula: MET
Molecular weightTheoretical: 149.211 Da
Chemical component information

ChemComp-MET:
METHIONINE / Methionine

+
Macromolecule #57: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER

MacromoleculeName: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / type: ligand / ID: 57 / Number of copies: 1 / Formula: GCP
Molecular weightTheoretical: 521.208 Da
Chemical component information

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.5 µm
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 46.4 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Initial angle assignmentType: OTHER / Details: Relion
Final angle assignmentType: OTHER / Details: Relion
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 12059
FSC plot (resolution estimation)

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more