EMDB-16127: Yeast 80S, ES7s delta, eIF5A, Stm1 containing

Basic information

Entry

Database: EMDB / ID: EMD-16127

• Title: Yeast 80S, ES7s delta, eIF5A, Stm1 containing

Sample

• Complex: Dormant yeast 80S ribosome
  • RNA: x 2 types
  • Protein or peptide: x 70 types
• Protein or peptide: x 7 types
• RNA: x 2 types
• Ligand: x 6 types

• Keywords: eIF5A / Stm1 / Rpl41A / delta ES7s / RIBOSOME

Function / homology

Function:

positive regulation of translational termination / positive regulation of translational elongation / ribosomal subunit / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / mTORC1-mediated signalling / ribosome-associated ubiquitin-dependent protein catabolic process / Protein hydroxylation / GDP-dissociation inhibitor activity / : / pre-mRNA 5'-splice site binding / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Ribosomal scanning and start codon recognition / response to cycloheximide / mRNA destabilization / Major pathway of rRNA processing in the nucleolus and cytosol / SRP-dependent cotranslational protein targeting to membrane / 90S preribosome / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / preribosome, large subunit precursor / L13a-mediated translational silencing of Ceruloplasmin expression / translation regulator activity / ribosomal large subunit export from nucleus / G-protein alpha-subunit binding / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of translational fidelity / positive regulation of protein kinase activity / translation elongation factor activity / rescue of stalled ribosome / maturation of SSU-rRNA / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / cellular response to amino acid starvation / small-subunit processome / cytosolic ribosome / protein kinase C binding / maintenance of translational fidelity / macroautophagy / modification-dependent protein catabolic process / ribosomal small subunit biogenesis / ribosomal large subunit assembly / small ribosomal subunit rRNA binding / protein tag activity / ribosomal small subunit assembly / rRNA processing / cytoplasmic stress granule / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / ribosome binding / ribosome biogenesis / cytoplasmic translation / small ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit / negative regulation of translation / nucleic acid binding / rRNA binding / protein ubiquitination / ribosome / structural constituent of ribosome / positive regulation of protein phosphorylation / ribonucleoprotein complex / translation / G protein-coupled receptor signaling pathway / negative regulation of gene expression / response to antibiotic / mRNA binding / ubiquitin protein ligase binding / nucleolus / mitochondrion / RNA binding / zinc ion binding / nucleoplasm / metal ion binding / nucleus / cytosol / cytoplasm

Domain/homology:

Hyaluronan / mRNA binding family / Translation elongation factor, IF5A, hypusine site / Eukaryotic initiation factor 5A hypusine signature. / Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold / Translation elongation factor IF5A-like / Translation elongation factor, IF5A C-terminal / Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold / Stm1-like, N-terminal / Stm1 / Hyaluronan/mRNA-binding protein / Hyaluronan / mRNA binding family / Ribosomal protein L41 / Ribosomal protein L41 / : / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S21e, conserved site / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S19e, conserved site / S27a-like superfamily / Ribosomal protein S26e / Ribosomal protein L29e / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S25 / Ribosomal protein S26e signature. / Ribosomal protein S17e, conserved site / : / Ribosomal protein S2, eukaryotic / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal L29e protein family / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S8e subdomain, eukaryotes / S25 ribosomal protein / Ribosomal protein L10e, conserved site / Ribosomal protein L10e / Ribosomal protein L27e, conserved site / Ribosomal protein S19A/S15e / Ribosomal protein S21e signature. / Ribosomal protein S30 / Ribosomal protein S3Ae, conserved site / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S8e, conserved site / 40S ribosomal protein S11, N-terminal / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S6, eukaryotic / Ribosomal protein L24e, conserved site / Ribosomal protein S7e / 40S ribosomal protein S4, C-terminal domain / Ribosomal protein L34e, conserved site / Ribosomal protein L1, conserved site / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal S17 / Ribosomal protein S19e signature. / Ribosomal protein L44e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal protein S19e / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal L38e protein family / Ribosomal L22e protein family / Ribosomal protein L23/L25, N-terminal / 40S Ribosomal protein S10 / 60S ribosomal protein L35 / Ribosomal protein L1 / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S27 / Ribosomal protein S28e conserved site / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S28e / Ribosomal protein L35Ae, conserved site / Ribosomal protein L30e, conserved site / 40S ribosomal protein S4 C-terminus / Ribosomal protein S4e, N-terminal / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal_S17 N-terminal / Plectin/S10, N-terminal / Ribosomal protein S3Ae / Ribosomal S3Ae family / Ribosomal protein L44 / Ribosomal protein L34Ae / Ribosomal protein S7e / Ribosomal protein L23, N-terminal domain / Ribosomal protein L13e, conserved site

Component:

40S ribosomal protein S8 / ES17 / RPS5 isoform 1 / Small ribosomal subunit protein eS1 / RPS22A isoform 1 / RPS31 isoform 1 / RPL38 isoform 1 / RPL10 isoform 1 / RPS29A isoform 1 / RPS20 isoform 1 / RPS2 isoform 1 / 60S ribosomal protein L29 / RPL24A isoform 1 / RPL11B isoform 1 / 40S ribosomal protein S25 / 40S ribosomal protein S26 / RPL5 isoform 1 / RPL32 isoform 1 / 40S ribosomal protein S3 / RPL4A isoform 1 / Large ribosomal subunit protein uL3 / RPS15 isoform 1 / RPS28A isoform 1 / Ribosomal protein L15 / STM1 isoform 1 / RPL9A isoform 1 / Eukaryotic translation initiation factor 5A / Small ribosomal subunit protein uS4A / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL22A / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein eS19A / Small ribosomal subunit protein eS21A / Large ribosomal subunit protein eL27A / Large ribosomal subunit protein eL31A / Ubiquitin-ribosomal protein eL40A fusion protein / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein eL43A / Large ribosomal subunit protein eL42A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A / Small ribosomal subunit protein eS30A / Small ribosomal subunit protein eS4A / Small ribosomal subunit protein eS6A / Large ribosomal subunit protein uL14A / Large ribosomal subunit protein uL1A / Large ribosomal subunit protein uL2A / Small ribosomal subunit protein uS17A / Large ribosomal subunit protein eL18A / Small ribosomal subunit protein uS9A / Small ribosomal subunit protein uS13A / Large ribosomal subunit protein eL19A / Large ribosomal subunit protein uL29A / Small ribosomal subunit protein eS32B / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein eL8A / Large ribosomal subunit protein uL13A / Small ribosomal subunit protein eS7A / Small ribosomal subunit protein uS2A / Small ribosomal subunit protein eS27A / Large ribosomal subunit protein eL14A / Small ribosomal subunit protein RACK1 / Small ribosomal subunit protein uS11B / Large ribosomal subunit protein eL37A / Large ribosomal subunit protein eL34A / Large ribosomal subunit protein eL21A / Small ribosomal subunit protein eS10A / Large ribosomal subunit protein eL13A

• Biological species: Saccharomyces cerevisiae (brewer's yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 2.4 Å
• Authors: Dimitrova-Paternoga L / Paternoga H / Wilson DN

Funding support

Germany, Switzerland, 2 items

Organization	Grant number	Country
German Research Foundation (DFG)	WI3285/11-1	Germany
Swiss National Science Foundation	188969	Switzerland

• Citation: Journal: Nucleic Acids Res / Year: 2024; Title: Evolving precision: rRNA expansion segment 7S modulates translation velocity and accuracy in eukaryal ribosomes.; Authors: Robert Rauscher / Cristian Eggers / Lyudmila Dimitrova-Paternoga / Vaishnavi Shankar / Alessia Rosina / Marina Cristodero / Helge Paternoga / Daniel N Wilson / Sebastian A Leidel / Norbert Polacek / ; Abstract: Ribosome-enhanced translational miscoding of the genetic code causes protein dysfunction and loss of cellular fitness. During evolution, open reading frame length increased, necessitating mechanisms for enhanced translation fidelity. Indeed, eukaryal ribosomes are more accurate than bacterial counterparts, despite their virtually identical, conserved active centers. During the evolution of eukaryotic organisms ribosome expansions at the rRNA and protein level occurred, which potentially increases the options for translation regulation and cotranslational events. Here we tested the hypothesis that ribosomal RNA expansions can modulate the core function of the ribosome, faithful protein synthesis. We demonstrate that a short expansion segment present in all eukaryotes' small subunit, ES7S, is crucial for accurate protein synthesis as its presence adjusts codon-specific velocities and guarantees high levels of cognate tRNA selection. Deletion of ES7S in yeast enhances mistranslation and causes protein destabilization and aggregation, dramatically reducing cellular fitness. Removal of ES7S did not alter ribosome architecture but altered the structural dynamics of inter-subunit bridges thus affecting A-tRNA selection. Exchanging the yeast ES7S sequence with the human ES7S increases accuracy whereas shortening causes the opposite effect. Our study demonstrates that ES7S provided eukaryal ribosomes with higher accuracy without perturbing the structurally conserved decoding center.

History

Deposition	Nov 12, 2022	-
Header (metadata) release	Jan 10, 2024	-
Map release	Jan 10, 2024	-
Update	Feb 21, 2024	-
Current status	Feb 21, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_16127.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 0.783 Å

Density

Contour Level	By AUTHOR: 0.0143
Minimum - Maximum	-0.016028821 - 0.08283466
Average (Standard dev.)	0.00041571428 (±0.0037988264)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 480 480 480 Spacing 480 480 480
Cell	A=B=C: 375.84 Å α=β=γ: 90.0 °

Supplemental data

Half map: #2

• File: emd_16127_half_map_1.map

Half map: #1

• File: emd_16127_half_map_2.map

Sample components

Entire : Dormant yeast 80S ribosome

• Entire: Name: Dormant yeast 80S ribosome

Components

• Complex: Dormant yeast 80S ribosome
  • RNA: 5S ribosomal RNA
  • RNA: 5.8S ribosomal RNA
  • Protein or peptide: 60S ribosomal protein L1-ARibosome
  • Protein or peptide: 60S ribosomal protein L41-ARibosome
  • Protein or peptide: Small ribosomal subunit protein uS2A
  • Protein or peptide: Small ribosomal subunit protein eS1
  • Protein or peptide: RPS2 isoform 1
  • Protein or peptide: 40S ribosomal protein S3
  • Protein or peptide: Small ribosomal subunit protein eS4A
  • Protein or peptide: Rps5p
  • Protein or peptide: Small ribosomal subunit protein eS6A
  • Protein or peptide: 40S ribosomal protein S7-ARibosome
  • Protein or peptide: 40S ribosomal protein S8
  • Protein or peptide: Small ribosomal subunit protein uS4A
  • Protein or peptide: Small ribosomal subunit protein eS10A
  • Protein or peptide: Small ribosomal subunit protein uS17A
  • Protein or peptide: 40S ribosomal protein S13
  • Protein or peptide: 40S ribosomal protein S14-BRibosome
  • Protein or peptide: RPS15 isoform 1
  • Protein or peptide: 40S ribosomal protein S16-ARibosome
  • Protein or peptide: ES17
  • Protein or peptide: 40S ribosomal protein S18-ARibosome
  • Protein or peptide: 40S ribosomal protein S19-ARibosome
  • Protein or peptide: RPS20 isoform 1
  • Protein or peptide: Small ribosomal subunit protein eS21A
  • Protein or peptide: RPS22A isoform 1
  • Protein or peptide: 40S ribosomal protein S23-ARibosome
  • Protein or peptide: 40S ribosomal protein S24-ARibosome
  • Protein or peptide: 40S ribosomal protein S25
  • Protein or peptide: RPS26B isoform 1
  • Protein or peptide: 40S ribosomal protein S27-ARibosome
  • Protein or peptide: RPS28A isoform 1
  • Protein or peptide: RPS29A isoform 1
  • Protein or peptide: 40S ribosomal protein S30-ARibosome
  • Protein or peptide: RPS31 isoform 1
  • Protein or peptide: Small ribosomal subunit protein RACK1
  • Protein or peptide: 60S ribosomal protein L3
  • Protein or peptide: RPL5 isoform 1
  • Protein or peptide: 60S ribosomal protein L6-ARibosome
  • Protein or peptide: 60S ribosomal protein L7-ARibosome
  • Protein or peptide: 60S ribosomal protein L8-ARibosome
  • Protein or peptide: RPL9A isoform 1
  • Protein or peptide: RPL11B isoform 1
  • Protein or peptide: 60S ribosomal protein L13-ARibosome
  • Protein or peptide: 60S ribosomal protein L14-ARibosome
  • Protein or peptide: 60S ribosomal protein L16-ARibosome
  • Protein or peptide: 60S ribosomal protein L17-ARibosome
  • Protein or peptide: 60S ribosomal protein L18-ARibosome
  • Protein or peptide: 60S ribosomal protein L19-ARibosome
  • Protein or peptide: 60S ribosomal protein L20-ARibosome
  • Protein or peptide: 60S ribosomal protein L21-ARibosome
  • Protein or peptide: 60S ribosomal protein L22-ARibosome
  • Protein or peptide: 60S ribosomal protein L23-ARibosome
  • Protein or peptide: RPL24A isoform 1
  • Protein or peptide: 60S ribosomal protein L25
  • Protein or peptide: Large ribosomal subunit protein uL24A
  • Protein or peptide: 60S ribosomal protein L27-ARibosome
  • Protein or peptide: 60S ribosomal protein L28
  • Protein or peptide: 60S ribosomal protein L30
  • Protein or peptide: 60S ribosomal protein L31-ARibosome
  • Protein or peptide: RPL32 isoform 1
  • Protein or peptide: 60S ribosomal protein L33-ARibosome
  • Protein or peptide: 60S ribosomal protein L35-ARibosome
  • Protein or peptide: 60S ribosomal protein L36-ARibosome
  • Protein or peptide: RPL38 isoform 1
  • Protein or peptide: 60S ribosomal protein L39
  • Protein or peptide: 60S ribosomal protein L40-ARibosome
  • Protein or peptide: Large ribosomal subunit protein eL41B
  • Protein or peptide: 60S ribosomal protein L42-ARibosome
  • Protein or peptide: 60S ribosomal protein L43-ARibosome
  • Protein or peptide: STM1 isoform 1
  • Protein or peptide: Eukaryotic translation initiation factor 5A
• Protein or peptide: 60S ribosomal protein L2-ARibosome
• Protein or peptide: RPL4A isoform 1
• Protein or peptide: RPL10 isoform 1
• Protein or peptide: Ribosomal protein L15
• Protein or peptide: 60S ribosomal protein L29
• Protein or peptide: Large ribosomal subunit protein eL34A
• Protein or peptide: Large ribosomal subunit protein eL37A
• RNA: 25S ribosomal RNA
• RNA: 18S ribosomal RNA
• Ligand: MAGNESIUM ION
• Ligand: POTASSIUM IONPotassium
• Ligand: 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione
• Ligand: SPERMIDINE
• Ligand: ZINC ION
• Ligand: water

Supramolecule #1: Dormant yeast 80S ribosome

• Supramolecule: Name: Dormant yeast 80S ribosome / type: complex / ID: 1 / Parent: 0 ; Macromolecule list: #11-#12, #1, #10, #15-#18, #80, #2, #19-#22, #3, #23-#24, #4-#5, #25-#26, #6, #27, #7, #28-#31, #8, #32-#33, #9, #34-#35, #81, #36, #38, #40-#44, #46-#48, #50-#62, #64-#67, #69-#70, #72-#79
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 3.3 MDa

Macromolecule #1: 60S ribosomal protein L1-A

• Macromolecule: Name: 60S ribosomal protein L1-A / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 24.524799 KDa

Sequence

String:

MSKITSSQVR EHVKELLKYS NETKKRNFLE TVELQVGLKN YDPQRDKRFS GSLKLPNCPR PNMSICIFGD AFDVDRAKSC GVDAMSVDD LKKLNKNKKL IKKLSKKYNA FIASEVLIKQ VPRLLGPQLS KAGKFPTPVS HNDDLYGKVT DVRSTIKFQL K KVLCLAVA VGNVEMEEDV LVNQILMSVN FFVSLLKKNW QNVGSLVVKS SMGPAFRLY

UniProtKB: Large ribosomal subunit protein uL1A

Macromolecule #2: Rps5p

• Macromolecule: Name: Rps5p / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 22.908338 KDa

Sequence

String:

FTPVVLATPI PEEVQQAQTE IKLFNKWSFE EVEVKDASLV DYVQVRQPIF VAHTAGRYAN KRFRKAQCPI IERLTNSLMM NGRNNGKKL KAVRIIKHTL DIINVLTDQN PIQVVVDAIT NTGPREDTTR VGGGGAARRQ AVDVSPLRRV NQAIALLTIG A REAAFRNI KTIAETLAEE LINAAKGSST SYAIKKKDEL ERVAKSNR

UniProtKB: RPS5 isoform 1

Macromolecule #3: Small ribosomal subunit protein eS10A

• Macromolecule: Name: Small ribosomal subunit protein eS10A / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 11.585125 KDa

Sequence

String:

MLMPKEDRNK IHQYLFQEGV VVAKKDFNQA KHEEIDTKNL YVIKALQSLT SKGYVKTQFS WQYYYYTLTE EGVEYLREYL NLPEHIVPA TYIQERN

UniProtKB: Small ribosomal subunit protein eS10A

Macromolecule #4: 40S ribosomal protein S14-B

• Macromolecule: Name: 40S ribosomal protein S14-B / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 13.446426 KDa

Sequence

String:

SQVFGVARIY ASFNDTFVHV TDLSGKETIA RVTGGMKVKA DRDESSPYAA MLAAQDVAAK CKEVGITAVH VKIRATGGTR TKTPGPGGQ AALRALARSG LRIGRIEDVT PVPSDSTRKK GGRRGRRL

UniProtKB: Small ribosomal subunit protein uS11B

Macromolecule #5: RPS15 isoform 1

• Macromolecule: Name: RPS15 isoform 1 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.15572 KDa

Sequence

String:

KKRVFKTHSY RGVDLEKLLE MSTEDFVKLA PARVRRRFAR GMTSKPAGFM KKLRAAKLAA PENEKPAPVR THMRNMIIVP EMIGSVVGI YNGKAFNQVE IRPEMLGHYL GEFSITYTPV RHGRA

UniProtKB: RPS15 isoform 1

Macromolecule #6: 40S ribosomal protein S18-A

• Macromolecule: Name: 40S ribosomal protein S18-A / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 16.940443 KDa

Sequence

String:

SLVVQEQGSF QHILRLLNTN VDGNIKIVYA LTTIKGVGRR YSNLVCKKAD VDLHKRAGEL TQEELERIVQ IMQNPTHYKI PAWFLNRQN DITDGKDYHT LANNVESKLR DDLERLKKIR AHRGIRHFWG LRVRGQHTKT TGRRRA

UniProtKB: Small ribosomal subunit protein uS13A

Macromolecule #7: RPS20 isoform 1

• Macromolecule: Name: RPS20 isoform 1 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 12.190175 KDa

Sequence

String:

QQQQIIKIRI TLTSTKVKQL ENVSSNIVKN AEQHNLVKKG PVRLPTKVLK ISTRKTPNGE GSKTWETYEM RIHKRYIDLE APVQIVKRI TQITIEPGVD VEVVVASN

UniProtKB: RPS20 isoform 1

Macromolecule #8: 40S ribosomal protein S25

• Macromolecule: Name: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 8.001377 KDa

Sequence

String:

AQHAVILDQE KYDRILKEVP TYRYVSVSVL VDRLKIGGSL ARIALRHLEK EGIIKPISKH SKQAIYTRAT

UniProtKB: 40S ribosomal protein S25

Macromolecule #9: RPS28A isoform 1

• Macromolecule: Name: RPS28A isoform 1 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 7.116281 KDa

Sequence

String:

TPVTLAKVIK VLGRTGSRGG VTQVRVEFLE DTSRTIVRNV KGPVRENDIL VLMESEREAR RLR

UniProtKB: RPS28A isoform 1

Macromolecule #10: 60S ribosomal protein L41-A

• Macromolecule: Name: 60S ribosomal protein L41-A / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 2.108656 KDa

Sequence

String:

MRARWRKKRT RRLKR

Macromolecule #15: Small ribosomal subunit protein uS2A

• Macromolecule: Name: Small ribosomal subunit protein uS2A / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 27.920133 KDa

Sequence

String:

SLPATFDLTP EDAQLLLAAN THLGARNVQV HQEPYVFNAR PDGVHVINVG KTWEKLVLAA RIIAAIPNPE DVVAISSRTF GQRAVLKFA AHTGATPIAG RFTPGSFTNY ITRSFKEPRL VIVTDPRSDA QAIKEASYVN IPVIALTDLD SPSEFVDVAI P CNNRGKHS IGLIWYLLAR EVLRLRGALV DRTQPWSIMP DLYFYRDPEE VEQQVAEEAT TEEAGEEEAK EEVTEEQAEA TE WAEENAD NVEW

UniProtKB: Small ribosomal subunit protein uS2A

Macromolecule #16: Small ribosomal subunit protein eS1

• Macromolecule: Name: Small ribosomal subunit protein eS1 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 24.312199 KDa

Sequence

String:

VVDPFTRKEW FDIKAPSTFE NRNVGKTLVN KSTGLKSASD ALKGRVVEVC LADLQGSEDH SFRKIKLRVD EVQGKNLLTN FHGMDFTTD KLRSMVRKWQ TLIEANVTVK TSDDYVLRIF AIAFTRKQAN QVKRHSYAQS SHIRAIRKVI SEILTKEVQG S TLAQLTSK LIPEVINKEI ENATKDIFPL QNIHVRKVKL LKQPKFDVGA LMALHG

UniProtKB: Small ribosomal subunit protein eS1

Macromolecule #17: RPS2 isoform 1

• Macromolecule: Name: RPS2 isoform 1 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 23.212979 KDa

Sequence

String:

GWVPVTKLGR LVKAGKITTI EEIFLHSLPV KEFQIIDTLL PGLQDEVMNI KPVQKQTRAG QRTRFKAVVV VGDSNGHVGL GIKTAKEVA GAIRAGIIIA KLSVIPIRRG YWGTNLGQPH SLATKTTGKC GSVTVRLIPA PRGSGIVASP AVKKLLQLAG V EDVYTQSN GKTRTLENTL KAAFVAIGNT YGFLTPNLWA EQPLPVSPLD IYSDEASAQ

UniProtKB: RPS2 isoform 1

Macromolecule #18: 40S ribosomal protein S3

• Macromolecule: Name: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 24.702791 KDa

Sequence

String:

ALISKKRKLV ADGVFYAELN EFFTRELAEE GYSGVEVRVT PTKTEVIIRA TRTQDVLGEN GRRINELTLL VQKRFKYAPG TIVLYAERV QDRGLSAVAQ AESMKFKLLN GLAIRRAAYG VVRYVMESGA KGCEVVVSGK LRAARAKAMK FADGFLIHSG Q PVNDFIDT ATRHVLMRQG VLGIKVKIMR DPAKSRTGPK ALPDAVTIIE PKEEEPILAP SVKDY

UniProtKB: 40S ribosomal protein S3

Macromolecule #19: Small ribosomal subunit protein eS6A

• Macromolecule: Name: Small ribosomal subunit protein eS6A / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 25.895072 KDa

Sequence

String:

MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRD GERKRKSVRG AIVGPDLAVL ALVIVKKGEQ ELEGLTDTTV PKRLGPKRAN NIRKFFGLSK EDDVRDFVIR R EVTKGEKT YTKAPKIQRL VTPQRLQRKR HQRALKVRNA QAQREAAAEY AQLLAKRLSE RKAEKAEI

UniProtKB: Small ribosomal subunit protein eS6A

Macromolecule #20: 40S ribosomal protein S7-A

• Macromolecule: Name: 40S ribosomal protein S7-A / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.000492 KDa

Sequence

String:

PQAKILSQAP TELELQVAQA FVELENSSPE LKAELRPLQF KSIREIDVAG GKKALAIFVP VPSLAGFHKV QTKLTRELEK KFQDRHVIF LAERRILPKP SRTSRQVQKR PRSRTLTAVH DKILEDLVFP TEIVGKRVRY LVGGNKIQKV LLDSKDVQQI D YKLESFQA VYNKLTGKQI VFEIPS

UniProtKB: Small ribosomal subunit protein eS7A

Macromolecule #21: 40S ribosomal protein S8

• Macromolecule: Name: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.14718 KDa

Sequence

String:

GISRDSRHKR SATGAKRAQF RKKRKFELGR QPANTKIGAK RIHSVRTRGG NKKYRALRIE TGNFSWASEG ISKKTRIAGV VYHPSNNEL VRTNTLTKAA IVQIDATPFR QWFEAHYGQT LGKKSKNAER KWAARAASAK IESSVESQFS AGRLYACISS R PGQSGRCD GYILEGEELA FYLRRLTAKK

UniProtKB: 40S ribosomal protein S8

Macromolecule #22: Small ribosomal subunit protein uS4A

• Macromolecule: Name: Small ribosomal subunit protein uS4A / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.210662 KDa

Sequence

String:

PRAPRTYSKT YSTPKRPYES SRLDAELKLA GEFGLKNKKE IYRISFQLSK IRRAARDLLT RDEKDPKRLF EGNALIRRLV RVGVLSEDK KKLDYVLALK VEDFLERRLQ TQVYKLGLAK SVHHARVLIT QRHIAVGKQI VNIPSFMVRL DSEKHIDFAP T SPFGGARP GRVARRNAAR KAEASGE

UniProtKB: Small ribosomal subunit protein uS4A

Macromolecule #23: Small ribosomal subunit protein uS17A

• Macromolecule: Name: Small ribosomal subunit protein uS17A / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 17.668766 KDa

Sequence

String:

STELTVQSER AFQKQPHIFN NPKVKTSKRT KRWYKNAGLG FKTPKTAIEG SYIDKKCPFT GLVSIRGKIL TGTVVSTKMH RTIVIRRAY LHYIPKYNRY EKRHKNVPVH VSPAFRVQVG DIVTVGQCRP ISKTVRFNVV KVSAAAAKAN KQFAKF

UniProtKB: Small ribosomal subunit protein uS17A

Macromolecule #24: 40S ribosomal protein S13

• Macromolecule: Name: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 16.928748 KDa

Sequence

String:

GRMHSAGKGI SSSAIPYSRN APAWFKLSSE SVIEQIVKYA RKGLTPSQIG VLLRDAHGVT QARVITGNKI MRILKSNGLA PEIPEDLYY LIKKAVSVRK HLERNRKDKD AKFRLILIES RIHRLARYYR TVAVLPPNWK YESATASALV N

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #25: 40S ribosomal protein S16-A

• Macromolecule: Name: 40S ribosomal protein S16-A / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 15.659216 KDa

Sequence

String:

AVPSVQTFGK KKSATAVAHV KAGKGLIKVN GSPITLVEPE ILRFKVYEPL LLVGLDKFSN IDIRVRVTGG GHVSQVYAIR QAIAKGLVA YHQKYVDEQS KNELKKAFTS YDRTLLIADS RRPEPKKFGG KGARSRFQKS YR

UniProtKB: Small ribosomal subunit protein uS9A

Macromolecule #26: ES17

• Macromolecule: Name: ES17 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 13.768985 KDa

Sequence

String:

GRVRTKTVKR ASKALIERYY PKLTLDFQTN KRLCDEIATI QSKRLRNKIA GYTTHLMKRI QKGPVRGISF KLQEEERERK DQYVPEVSR SNGVLNVDNQ TSDLVKSLGL KLPLSVINVS A

UniProtKB: ES17

Macromolecule #27: 40S ribosomal protein S19-A

• Macromolecule: Name: 40S ribosomal protein S19-A / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 15.81093 KDa

Sequence

String:

PGVSVRDVAA QDFINAYASF LQRQGKLEVP GYVDIVKTSS GNEMPPQDAE GWFYKRAASV ARHIYMRKQV GVGKLNKLYG GAKSRGVRP YKHIDASGSI NRKVLQALEK IGIVEISPKG GRRISENGQR DLDRIAAQTL EEDE

UniProtKB: Small ribosomal subunit protein eS19A

Macromolecule #28: Small ribosomal subunit protein eS21A

• Macromolecule: Name: Small ribosomal subunit protein eS21A / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 9.758829 KDa

Sequence

String:

MENDKGQLVE LYVPRKCSAT NRIIKADDHA SVQINVAKVD EEGRAIPGEY VTYALSGYVR SRGESDDSLN RLAQNDGLLK NVWSYSR

UniProtKB: Small ribosomal subunit protein eS21A

Macromolecule #29: RPS22A isoform 1

• Macromolecule: Name: RPS22A isoform 1 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.518867 KDa

Sequence

String:

TRSSVLADAL NAINNAEKTG KRQVLIRPSS KVIIKFLQVM QKHGYIGEFE YIDDHRSGKI VVQLNGRLNK CGVISPRFNV KIGDIEKWT ANLLPARQFG YVILTTSAGI MDHEEARRKH VSGKILGFVY

UniProtKB: RPS22A isoform 1

Macromolecule #30: 40S ribosomal protein S23-A

• Macromolecule: Name: 40S ribosomal protein S23-A / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 15.942699 KDa

Sequence

String:

GKGKPRGLNS ARKLRVHRRN NRWAENNYKK RLLGTAFKSS PFGGSSHAKG IVLEKLGIES KQPNSAIRKC VRVQLIKNGK KVTAFVPND GCLNFVDEND EVLLAGFGRK GKAKGDIPGV RFKVVKVSGV SLLALWKEKK EKPRS

UniProtKB: Small ribosomal subunit protein uS12A

Macromolecule #31: 40S ribosomal protein S24-A

• Macromolecule: Name: 40S ribosomal protein S24-A / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 15.23165 KDa

Sequence

String:

SDAVTIRTRK VISNPLLARK QFVVDVLHPN RANVSKDELR EKLAEVYKAE KDAVSVFGFR TQFGGGKSVG FGLVYNSVAE AKKFEPTYR LVRYGLAEKV EKASRQQRKQ KKNRDKKIFG TGKRLAKKVA RRNAD

UniProtKB: Small ribosomal subunit protein eS24A

Macromolecule #32: RPS26B isoform 1

• Macromolecule: Name: RPS26B isoform 1 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 11.022989 KDa

Sequence

String:

PKKRASNGRN KKGRGHVKPV RCVNCSKSIP KDKAIKRMAI RNIVEAAAVR DLSEASVYPE YALPKTYNKL HYCVSCAIHA RIVRVRSRE DRKNRAPP

UniProtKB: 40S ribosomal protein S26

Macromolecule #33: 40S ribosomal protein S27-A

• Macromolecule: Name: 40S ribosomal protein S27-A / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 8.762195 KDa

Sequence

String:

VLVQDLLHPT AASEARKHKL KTLVQGPRSY FLDVKCPGCL NITTVFSHAQ TAVTCESCST ILCTPTGGKA KLSEGTSFRR K

UniProtKB: Small ribosomal subunit protein eS27A

Macromolecule #34: RPS29A isoform 1

• Macromolecule: Name: RPS29A isoform 1 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 6.335303 KDa

Sequence

String:

ENVWFSHPRR YGKGSRQCRV CSSHTGLIRK YGLNICRQCF REKANDIGFN KFR

UniProtKB: RPS29A isoform 1

Macromolecule #35: 40S ribosomal protein S30-A

• Macromolecule: Name: 40S ribosomal protein S30-A / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 6.779086 KDa

Sequence

String:

AKVHGSLARA GKVKSQTPKV EKTEKPKKPK GRAYKRLLYT RRFVNVTLVN GKRRMNPGPS

UniProtKB: Small ribosomal subunit protein eS30A

Macromolecule #36: Small ribosomal subunit protein RACK1

• Macromolecule: Name: Small ribosomal subunit protein RACK1 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 34.710023 KDa

Sequence

String:

ASNEVLVLRG TLEGHNGWVT SLATSAGQPN LLLSASRDKT LISWKLTGDD QKFGVPVRSF KGHSHIVQDC TLTADGAYAL SASWDKTLR LWDVATGETY QRFVGHKSDV MSVDIDKKAS MIISGSRDKT IKVWTIKGQC LATLLGHNDW VSQVRVVPNE K ADDDSVTI ISAGNDKMVK AWNLNQFQIE ADFIGHNSNI NTLTASPDGT LIASAGKDGE IMLWNLAAKK AMYTLSAQDE VF SLAFSPN RYWLAAATAT GIKVFSLDPQ YLVDDLRPEF AGYSKAAEPH AVSLAWSADG QTLFAGYTDN VIRVWQVMTA N

UniProtKB: Small ribosomal subunit protein RACK1

Macromolecule #37: 60S ribosomal protein L2-A

• Macromolecule: Name: 60S ribosomal protein L2-A / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 27.217289 KDa

Sequence

String:

GRVIRNQRKG AGSIFTSHTR LRQGAAKLRT LDYAERHGYI RGIVKQIVHD SGRGAPLAKV VFRDPYKYRL REEIFIANEG VHTGQFIYA GKKASLNVGN VLPLGSVPEG TIVSNVEEKP GDRGALARAS GNYVIIIGHN PDENKTRVRL PSGAKKVISS D ARGVIGVI AGGGRVDKPL LKAGRAFHKY RLKRNSWPKT RGVAMNPVDH PHGGGNHQHI GKASTISRGA VSGQKAGLIA AR RTGLLRG SQKTQ

UniProtKB: Large ribosomal subunit protein uL2A

Macromolecule #38: 60S ribosomal protein L3

• Macromolecule: Name: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 43.719602 KDa

Sequence

String:

SHRKYEAPRH GHLGFLPRKR AASIRARVKA FPKDDRSKPV ALTSFLGYKA GMTTIVRDLD RPGSKFHKRE VVEAVTVVDT PPVVVVGVV GYVETPRGLR SLTTVWAEHL SDEVKRRFYK NWYKSKKKAF TKYSAKYAQD GAGIERELAR IKKYASVVRV L VHTQIRKT PLAQKKAHLA EIQLNGGSIS EKVDWAREHF EKTVAVDSVF EQNEMIDAIA VTKGHGFEGV THRWGTKKLP RK THRGLRK VACIGAWHPA HVMWSVARAG QRGYHSRTSI NHKIYRVGKG DDEANGATSF DRTKKTITPM GGFVHYGEIK NDF IMVKGC IPGNRKRIVT LRKSLYTNTS RKALEEVSLK WIDTASKFGK GRFQTPAEKH AFMGTLKKDL

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #39: RPL4A isoform 1

• Macromolecule: Name: RPL4A isoform 1 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 39.027926 KDa

Sequence

String:

SRPQVTVHSL TGEATANALP LPAVFSAPIR PDIVHTVFTS VNKNKRQAYA VSEKAGHQTS AESWGTGRAV ARIPRVGGGG TGRSGQGAF GNMCRGGRMF APTKTWRKWN VKVNHNEKRY ATASAIAATA VASLVLARGH RVEKIPEIPL VVSTDLESIQ K TKEAVAAL KAVGAHSDLL KVLKSKKLRA GKGKYRNRRW TQRRGPLVVY AEDNGIVKAL RNVPGVETAN VASLNLLQLA PG AHLGRFV IWTEAAFTKL DQVWGSETVA SSKVGYTLPS HIISTSDVTR IINSSEIQSA IRPAGQATQK RTHVLKKNPL KNK QVLLRL NPYAKVFAAE KLGSKKAEKT GTKPAAVFTE TLKHD

UniProtKB: RPL4A isoform 1

Macromolecule #40: RPL5 isoform 1

• Macromolecule: Name: RPL5 isoform 1 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 33.505504 KDa

Sequence

String:

AFQKDAKSSA YSSRFQTPFR RRREGKTDYY QRKRLVTQHK AKYNTPKYRL VVRFTNKDII CQIISSTITG DVVLAAAYSH ELPRYGITH GLTNWAAAYA TGLLIARRTL QKLGLDETYK GVEEVEGEYE LTEAVEDGPR PFKVFLDIGL QRTTTGARVF G ALKGASDG GLYVPHSENR FPGWDFETEE IDPELLRSYI FGGHVSQYME ELADDDEERF SELFKGYLAD DIDADSLEDI YT SAHEAIR ADPAFKPTEK KFTKEQYAAE SKKYRQTKLS KEERAARVAA KIAALAGQ

UniProtKB: RPL5 isoform 1

Macromolecule #41: 60S ribosomal protein L6-A

• Macromolecule: Name: 60S ribosomal protein L6-A / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 17.579693 KDa

Sequence

String:

SAQKAPKWYP SEDVAALKKT RKAARPQKLR ASLVPGTVLI LLAGRFRGKR VVYLKHLEDN TLLISGPFKV NGVPLRRVNA RYVIATSTK VSVEGVNVEK FNVEYFAKEE IKAERVEDQK VVDKALIAEI KKTPLLKQYL SASFSLKNGD KPHMLKF

Macromolecule #42: 60S ribosomal protein L7-A

• Macromolecule: Name: 60S ribosomal protein L7-A / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 25.240377 KDa

Sequence

String:

AEQVAAERAA RKAANKEKRA IILERNAAYQ KEYETAERNI IQAKRDAKAA GSYYVEAQHK LVFVVRIKGI NKIPPKPRKV LQLLRLTRI NSGTFVKVTK ATLELLKLIE PYVAYGYPSY STIRQLVYKR GFGKINKQRV PLSDNAIIEA NLGKYGILSI D DLIHEIIT VGPHFKQANN FLWPFKLSNP SGGWGVPRKF KHFIQGGSFG NREEFINKLV KSMN

UniProtKB: Large ribosomal subunit protein uL30A

Macromolecule #43: 60S ribosomal protein L8-A

• Macromolecule: Name: 60S ribosomal protein L8-A / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 25.814998 KDa

Sequence

String:

NPLTHSTPKN FGIGQAVQPK RNLSRYVKWP EYVRVQRQKK ILSIRLKVPP TIAQFQYTLD RNTAAETFKL FNKYRPETAA EKKERLTKE AAAVAEGKSK QDASPKPYAV KYGLNHVVAL IENKKAKLVL IANDVDPIEL VVFLPALCKK MGVPYAIVKG K ARLGTLVN QKTSAVAALT EVRAEDEAAL AKLVSTIDAN FADKYDEVKK HWGGGILGNK AQAKMDKRAK NSDSA

UniProtKB: Large ribosomal subunit protein eL8A

Macromolecule #44: RPL9A isoform 1

• Macromolecule: Name: RPL9A isoform 1 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.376814 KDa

Sequence

String:

MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN QLIKVAVHNG GRKHVAALRT VKSLVDNMIT GVTKGYKYK MRYVYAHFPI NVNIVEKDGA KFIEVRNFLG DKKIRNVPVR DGVTIEFSTN VKDEIVLSGN SVEDVSQNAA D LQQICRVR NKDIRKFLDG IYVSHKGFIT E

UniProtKB: RPL9A isoform 1

Macromolecule #45: RPL10 isoform 1

• Macromolecule: Name: RPL10 isoform 1 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 24.192033 KDa

Sequence

String:

ARRPARCYRY QKNKPYPKSR YNRAVPDSKI RIYDLGKKKA TVDEFPLCVH LVSNELEQLS SEALEAARIC ANKYMTTVSG RDAFHLRVR VHPFHVLRIN KMQQGMRGAW GKPHGLAARV DIGQIIFSVR TKDSNKDVVV EGLRRARYKF PGQQKIILSK K WGFTNLDR PEYLKKREAG EVKDDGAFVK FLSKKGSLEN NIREFPEYFA A

UniProtKB: RPL10 isoform 1

Macromolecule #46: RPL11B isoform 1

• Macromolecule: Name: RPL11B isoform 1 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 19.1369 KDa

Sequence

String:

QNPMRDLKIE KLVLNISVGE SGDRLTRASK VLEQLSGQTP VQSKARYTVR TFGIRRNEKI AVHVTVRGPK AEEILERGLK VKEYQLRDR NFSATGNFGF GIDEHIDLGI KYDPSIGIFG MDFYVVMNRP GARVTRRKRC KGTVGNSHKT TKEDTVSWFK Q KYDADVLD

UniProtKB: RPL11B isoform 1

Macromolecule #47: 60S ribosomal protein L13-A

• Macromolecule: Name: 60S ribosomal protein L13-A / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.885234 KDa

Sequence

String:

AISKNLPILK NHFRKHWQER VKVHFDQAGK KVSRRNARAT RAAKIAPRPL DLLRPVVRAP TVKYNRKVRA GRGFTLAEVK AAGLTAAYA RTIGIAVDHR RQNRNQEIFD ANVQRLKEYQ SKIIVFPRNG KAPEAEQVLS AAATFPIAQP ATDVEARAVQ D NGESAFRT LRLARSEKKF RGIREKRARE KAEAE

UniProtKB: Large ribosomal subunit protein eL13A

Macromolecule #48: 60S ribosomal protein L14-A

• Macromolecule: Name: 60S ribosomal protein L14-A / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.976794 KDa

Sequence

String:

TDSIVKASNW RLVEVGRVVL IKKGQSAGKL AAIVEIIDQK KVLIDGPKAG VPRQAINLGQ VVLTPLTFAL PRGARTATVS KKWAAAAVC EKWAASSWAK KIAQRERRAA LTDFERFQVM VLRKQKRYTV KKALAKA

UniProtKB: Large ribosomal subunit protein eL14A

Macromolecule #49: Ribosomal protein L15

• Macromolecule: Name: Ribosomal protein L15 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 24.221982 KDa

Sequence

String:

GAYKYLEELQ RKKQSDVLRF LQRVRVWEYR QKNVIHRAAR PTRPDKARRL GYKAKQGFVI YRVRVRRGNR KRPVPKGATY GKPTNQGVN ELKYQRSLRA TAEERVGRRA ANLRVLNSYW VNQDSTYKYF EVILVDPQHK AIRRDARYNW ICDPVHKHRE A RGLTATGK KSRGINKGHK FNNTKAGRRK TWKRQNTLSL WRYR

UniProtKB: Ribosomal protein L15

Macromolecule #50: 60S ribosomal protein L16-A

• Macromolecule: Name: 60S ribosomal protein L16-A / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 22.028951 KDa

Sequence

String:

VEPVVVIDGK GHLVGRLASV VAKQLLNGQK IVVVRAEELN ISGEFFRNKL KYHDFLRKAT AFNKTRGPFH FRAPSRIFYK ALRGMVSHK TARGKAALER LKVFEGIPPP YDKKKRVVVP QALRVLRLKP GRKYTTLGKL STSVGWKYED VVAKLEAKRK V SSAEYYAK KRAFTKKVAS ANATAAESDV AKQLAALGY

UniProtKB: Large ribosomal subunit protein uL13A

Macromolecule #51: 60S ribosomal protein L17-A

• Macromolecule: Name: 60S ribosomal protein L17-A / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 20.458322 KDa

Sequence

String:

ARYGATSTNP AKSASARGSY LRVSFKNTRE TAQAINGWEL TKAQKYLEQV LDHQRAIPFR RFNSSIGRTA QGKEFGVTKA RWPAKSVKF VQGLLQNAAA NAEAKGLDAT KLYVSHIQVN QAPKQRRRTY RAHGRINKYE SSPSHIELVV TEKEEAVAKA A EKKVVRLT SRQRGRIAAQ KRIAA

UniProtKB: Large ribosomal subunit protein uL22A

Macromolecule #52: 60S ribosomal protein L18-A

• Macromolecule: Name: 60S ribosomal protein L18-A / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 20.478059 KDa

Sequence

String:

GIDHTSKQHK RSGHRTAPKS DNVYLKLLVK LYTFLARRTD APFNKVVLKA LFLSKINRPP VSVSRIARAL KQEGAANKTV VVVGTVTDD ARIFEFPKTT VAALRFTAGA RAKIVKAGGE CITLDQLAVR APKGQNTLIL RGPRNSREAV RHFGMGPHKG K APRILSTG RKFERARGRR RSKGFKV

UniProtKB: Large ribosomal subunit protein eL18A

Macromolecule #53: 60S ribosomal protein L19-A

• Macromolecule: Name: 60S ribosomal protein L19-A / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.631121 KDa

Sequence

String:

ANLRTQKRLA ASVVGVGKRK VWLDPNETSE IAQANSRNAI RKLVKNGTIV KKAVTVHSKS RTRAHAQSKR EGRHSGYGKR KGTREARLP SQVVWIRRLR VLRRLLAKYR DAGKIDKHLY HVLYKESKGN AFKHKRALVE HIIQAKADAQ REKALNEEAE A RRLKNRAA RDRRAQRVAE KRDALLKEDA

UniProtKB: Large ribosomal subunit protein eL19A

Macromolecule #54: 60S ribosomal protein L20-A

• Macromolecule: Name: 60S ribosomal protein L20-A / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 20.478852 KDa

Sequence

String:

MAHFKEYQVI GRRLPTESVP EPKLFRMRIF ASNEVIAKSR YWYFLQKLHK VKKASGEIVS INQINEAHPT KVKNFGVWVR YDSRSGTHN MYKEIRDVSR VAAVETLYQD MAARHRARFR SIHILKVAEI EKTADVKRQY VKQFLTKDLK FPLPHRVQKS T KTFSYKRP STFY

UniProtKB: Large ribosomal subunit protein eL20A

Macromolecule #55: 60S ribosomal protein L21-A

• Macromolecule: Name: 60S ribosomal protein L21-A / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 18.148066 KDa

Sequence

String:

GKSHGYRSRT RYMFQRDFRK HGAVHLSTYL KVYKVGDIVD IKANGSIQKG MPHKFYQGKT GVVYNVTKSS VGVIINKMVG NRYLEKRLN LRVEHIKHSK CRQEFLERVK ANAAKRAEAK AQGVAVQLKR QPAQPRESRI VSTEGNVPQT LAPVPYETFI

UniProtKB: Large ribosomal subunit protein eL21A

Macromolecule #56: 60S ribosomal protein L22-A

• Macromolecule: Name: 60S ribosomal protein L22-A / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 11.263863 KDa

Sequence

String:

QKIAKTFTVD VSSPTENGVF DPASYAKYLI DHIKVEGAVG NLGNAVTVTE DGTVVTVVST AKFSGKYLKY LTKKYLKKNQ LRDWIRFVS TKTNEYRLAF Y

UniProtKB: Large ribosomal subunit protein eL22A

Macromolecule #57: 60S ribosomal protein L23-A

• Macromolecule: Name: 60S ribosomal protein L23-A / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.362755 KDa

Sequence

String:

SGNGAQGTKF RISLGLPVGA IMNCADNSGA RNLYIIAVKG SGSRLNRLPA ASLGDMVMAT VKKGKPELRK KVMPAIVVRQ AKSWRRRDG VFLYFEDNAG VIANPKGEMK GSAITGPVGK ECADLWPRVA SNSGVVV

UniProtKB: Large ribosomal subunit protein uL14A

Macromolecule #58: RPL24A isoform 1

• Macromolecule: Name: RPL24A isoform 1 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 11.369424 KDa

Sequence

String:

MKVEIDSFSG AKIYPGRGTL FVRGDSKIFR FQNSKSASLF KQRKNPRRIA WTVLFRKHHK KGITEEVAKK RSRKTVKAQR PITGASLDL IKERRSLKP

UniProtKB: RPL24A isoform 1

Macromolecule #59: 60S ribosomal protein L25

• Macromolecule: Name: 60S ribosomal protein L25 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 13.771176 KDa

Sequence

String:

KALKVRTSAT FRLPKTLKLA RAPKYASKAV PHYNRLDSYK VIEQPITSET AMKKVEDGNI LVFQVSMKAN KYQIKKAVKE LYEVDVLKV NTLVRPNGTK KAYVRLTADY DALDIANRIG YI

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #60: Large ribosomal subunit protein uL24A

• Macromolecule: Name: Large ribosomal subunit protein uL24A / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.134588 KDa

Sequence

String:

AKQSLDVSSD RRKARKAYFT APSSQRRVLL SAPLSKELRA QYGIKALPIR RDDEVLVVRG SKKGQEGKIS SVYRLKFAVQ VDKVTKEKV NGASVPINLH PSKLVITKLH LDKDRKALIQ RKGGKLE

UniProtKB: Large ribosomal subunit protein uL24A

Macromolecule #61: 60S ribosomal protein L27-A

• Macromolecule: Name: 60S ribosomal protein L27-A / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 15.437166 KDa

Sequence

String:

AKFLKAGKVA VVVRGRYAGK KVVIVKPHDE GSKSHPFGHA LVAGIERYPL KVTKKHGAKK VAKRTKIKPF IKVVNYNHLL PTRYTLDVE AFKSVVSTET FEQPSQREEA KKVVKKAFEE RHQAGKNQWF FSKLRF

UniProtKB: Large ribosomal subunit protein eL27A

Macromolecule #62: 60S ribosomal protein L28

• Macromolecule: Name: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 16.630471 KDa

Sequence

String:

PSRFTKTRKH RGHVSAGKGR IGKHRKHPGG RGMAGGQHHH RINMDKYHPG YFGKVGMRYF HKQQAHFWKP VLNLDKLWTL IPEDKRDQY LKSASKETAP VIDTLAAGYG KILGKGRIPN VPVIVKARFV SKLAEEKIRA AGGVVELIA

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #63: 60S ribosomal protein L29

• Macromolecule: Name: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 6.302329 KDa

Sequence

String:

AKSKNHTAHN QTRKAHRNGI KKPKTYKYPS LKGVDPKFRR NHKHALHGTA KALAAA

UniProtKB: 60S ribosomal protein L29

Macromolecule #64: 60S ribosomal protein L30

• Macromolecule: Name: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 10.558344 KDa

Sequence

String:

SINQKLALVI KSGKYTLGYK STVKSLRQGK SKLIIIAANT PVLRKSELEY YAMLSKTKVY YFQGGNNELG TAVGKLFRVG VVSILEAGD SDILTTLA

UniProtKB: Large ribosomal subunit protein eL30

Macromolecule #65: 60S ribosomal protein L31-A

• Macromolecule: Name: 60S ribosomal protein L31-A / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 12.649754 KDa

Sequence

String:

LKDVVTREYT INLHKRLHGV SFKKRAPRAV KEIKKFAKLH MGTDDVRLAP ELNQAIWKRG VKGVEYRLRL RISRKRNEEE DAKNPLFSY VEPVLVASAK GLQTVVVEED

UniProtKB: Large ribosomal subunit protein eL31A

Macromolecule #66: RPL32 isoform 1

• Macromolecule: Name: RPL32 isoform 1 / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.478054 KDa

Sequence

String:

ASLPHPKIVK KHTKKFKRHH SDRYHRVAEN WRKQKGIDSV VRRRFRGNIS QPKIGYGSNK KTKFLSPSGH KTFLVANVKD LETLTMHTK TYAAEIAHNI SAKNRVVILA RAKALGIKVT NPKGRLAL

UniProtKB: RPL32 isoform 1

Macromolecule #67: 60S ribosomal protein L33-A

• Macromolecule: Name: 60S ribosomal protein L33-A / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 12.045935 KDa

Sequence

String:

AESHRLYVKG KHLSYQRSKR VNNPNVSLIK IEGVATPQDA QFYLGKRIAY VYRASKEVRG SKIRVMWGKV TRTHGNSGVV RATFRNNLP AKTFGASVRI FLYPSNI

UniProtKB: Large ribosomal subunit protein eL33A

Macromolecule #68: Large ribosomal subunit protein eL34A

• Macromolecule: Name: Large ribosomal subunit protein eL34A / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 12.479651 KDa

Sequence

String:

AQRVTFRRRN PYNTRSNKIK VVKTPGGILR AQHVKKLATR PKCGDCGSAL QGISTLRPRQ YATVSKTHKT VSRAYGGSRC ANCVKERII RAFLIEEQKI VKKVVKEQTE AA

UniProtKB: Large ribosomal subunit protein eL34A

Macromolecule #69: 60S ribosomal protein L35-A

• Macromolecule: Name: 60S ribosomal protein L35-A / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 13.811444 KDa

Sequence

String:

AGVKAYELRT KSKEQLASQL VDLKKELAEL KVQKLSRPSL PKIKTVRKSI ACVLTVINEQ QREAVRQLYK GKKYQPKDLR AKKTRALRR ALTKFEASQV TEKQRKKQIA FPQRKYAIKA

UniProtKB: Large ribosomal subunit protein uL29A

Macromolecule #70: 60S ribosomal protein L36-A

• Macromolecule: Name: 60S ribosomal protein L36-A / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 11.020063 KDa

Sequence

String:

TVKTGIAIGL NKGKKVTSMT PAPKISYKKG AASNRTKFVR SLVREIAGLS PYERRLIDLI RNSGEKRARK VAKKRLGSFT RAKAKVEEM NNIIAASRRH

UniProtKB: Large ribosomal subunit protein eL36A

Macromolecule #71: Large ribosomal subunit protein eL37A

• Macromolecule: Name: Large ribosomal subunit protein eL37A / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 9.675122 KDa

Sequence

String:

GKGTPSFGKR HNKSHTLCNR CGRRSFHVQK KTCSSCGYPA AKTRSYNWGA KAKRRHTTGT GRMRYLKHVS RRFKNGFQTG SASKAS

UniProtKB: Large ribosomal subunit protein eL37A

Macromolecule #72: RPL38 isoform 1

• Macromolecule: Name: RPL38 isoform 1 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 8.714363 KDa

Sequence

String:

AREITDIKQF LELTRRADVK TATVKINKKL NKAGKPFRQT KFKVRGSSSL YTLVINDAGK AKKLIQSLPP TLKVNRL

UniProtKB: RPL38 isoform 1

Macromolecule #73: 60S ribosomal protein L39

• Macromolecule: Name: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 6.227444 KDa

Sequence

String:

AAQKSFRIKQ KMAKAKKQNR PLPQWIRLRT NNTIRYNAKR RNWRRTKMNI

UniProtKB: Large ribosomal subunit protein eL39

Macromolecule #74: 60S ribosomal protein L40-A

• Macromolecule: Name: 60S ribosomal protein L40-A / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 6.032321 KDa

Sequence

String:

IIEPSLKALA SKYNCDKSVC RKCYARLPPR ATNCRKRKCG HTNQLRPKKK LK

UniProtKB: Ubiquitin-ribosomal protein eL40A fusion protein

Macromolecule #75: Large ribosomal subunit protein eL41B

• Macromolecule: Name: Large ribosomal subunit protein eL41B / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 3.354243 KDa

Sequence

String:

MRAKWRKKRT RRLKRKRRKV RARSK

UniProtKB: Small ribosomal subunit protein eS32B

Macromolecule #76: 60S ribosomal protein L42-A

• Macromolecule: Name: 60S ribosomal protein L42-A / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 12.115462 KDa

Sequence

String:

VNVPKTRKTY CKGKTCRKHT QHKVTQYKAG KASLFAQGKR RYDRKQSGFG GQTKPVFHKK AKTTKKVVLR LECVKCKTRA QLTLKRCKH FELGGEKKQK GQALQF

UniProtKB: Large ribosomal subunit protein eL42A

Macromolecule #77: 60S ribosomal protein L43-A

• Macromolecule: Name: 60S ribosomal protein L43-A / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 9.981756 KDa

Sequence

String:

AKRTKKVGIT GKYGVRYGSS LRRQVKKLEI QQHARYDCSF CGKKTVKRGA AGIWTCSCCK KTVAGGAYTV STAAAATVRS TIRRLREMV EA

UniProtKB: Large ribosomal subunit protein eL43A

Macromolecule #78: STM1 isoform 1

• Macromolecule: Name: STM1 isoform 1 / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 12.767008 KDa

Sequence

String:

PPKEIVKSNT SSKKADVPPP SADPSKARKN RPRPSGNEGA IRDKTAGRRN NRSKDVTDSA TTKKSNTRRA TDRHSRTGKT DTKKKVNQG WGDDKKELSA EKEAQADAAA EIAEDAAEA

UniProtKB: STM1 isoform 1

Macromolecule #79: Eukaryotic translation initiation factor 5A

• Macromolecule: Name: Eukaryotic translation initiation factor 5A / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 15.741892 KDa

Sequence

String:

TADAGSSATY PMQCSALRKN GFVVIKSRPC KIVDMSTSKT G(5CT)HGHAKVHL VAIDIFTGKK LEDLSPSTHN MEVPVV KRN EYQLLDIDDG FLSLMNMDGD TKDDVKAPEG ELGDSLQTAF DEGKDLMVTI ISAMGEEAAI SFKEAA

UniProtKB: Eukaryotic translation initiation factor 5A

Macromolecule #80: Small ribosomal subunit protein eS4A

• Macromolecule: Name: Small ribosomal subunit protein eS4A / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 29.338133 KDa

Sequence

String:

ARGPKKHLKR LAAPHHWLLD KLSGCYAPRP SAGPHKLRES LPLIVFLRNR LKYALNGREV KAILMQRHVK VDGKVRTDTT YPAGFMDVI TLDATNENFR LVYDVKGRFA VHRITDEEAS YKLGKVKKVQ LGKKGVPYVV THDGRTIRYP DPNIKVNDTV K IDLASGKI TDFIKFDAGK LVYVTGGRNL GRIGTIVHKE RHDGGFDLVH IKDSLDNTFV TRLNNVFVIG EQGKPYISLP KG KGIKLSI AEERDRRRAQ QGL

UniProtKB: Small ribosomal subunit protein eS4A

Macromolecule #81: RPS31 isoform 1

• Macromolecule: Name: RPS31 isoform 1 / type: protein_or_peptide / ID: 81 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 8.101675 KDa

Sequence

String:

KKVYTTPKKI KHKHKKVKLA VLSYYKVDAE GKVTKLRREC SNPTCGAGVF LANHKDRLYC GKCHSVYKVN A

UniProtKB: RPS31 isoform 1

Macromolecule #11: 5S ribosomal RNA

• Macromolecule: Name: 5S ribosomal RNA / type: rna / ID: 11 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 38.951105 KDa

Sequence

String:

GGUUGCGGCC AUAUCUACCA GAAAGCACCG UUUCCCGUCC GAUCAACUGU AGUUAAGCUG GUAAGAGCCU GACCGAGUAG UGUAGUGGG UGACCAUACG CGAAACUCAG GUGCUGCAAU CU

GENBANK: GENBANK: CP022977.1

Macromolecule #12: 5.8S ribosomal RNA

• Macromolecule: Name: 5.8S ribosomal RNA / type: rna / ID: 12 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 50.682922 KDa

Sequence

String:

AAACUUUCAA CAACGGAUCU CUUGGUUCUC GCAUCGAUGA AGAACGCAGC GAAAUGCGAU ACGUAAUGUG AAUUGCAGAA UUCCGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCCC CUUGGUAUUC CAGGGGGCAU GCCUGUUUGA GCGUCAUUU

GENBANK: GENBANK: MG817620.1

Macromolecule #13: 25S ribosomal RNA

• Macromolecule: Name: 25S ribosomal RNA / type: rna / ID: 13 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 1.022006562 MDa

Sequence

String:

UUGACCUCAA AUCAGGUAGG AGUACCCGCU GAACUUAAGC AUAUCAAUAA GCGGAGGAAA AGAAACCAAC CGGGAUUGCC UUAGUAACG GCGAGUGAAG CGGCAAAAGC UCAAAUUUGA AAUCUGGUAC CUUCGGUGCC CGAGUUGUAA UUUGGAGAGG G CAACUUUG GGGCCGUUCC UUGUCUAUGU UCCUUGGAAC AGGACGUCAU AGAGGGUGAG AAUCCCGUGU GGCGAGGAGU GC GGUUCUU UGUAAAGUGC CUUCGAAGAG UCGAGUUGUU UGGGAAUGCA GCUCUAAGUG GGUGGUAAAU UCCAUCUAAA GCU AAAUAU UGGCGAGAGA CCGAUAGCGA ACAAGUACAG UGAUGGAAAG AUGAAAAGAA CUUUGAAAAG AGAGUGAAAA AGUA CGUGA AAUUGUUGAA AGGGAAGGGC AUUUGAUCAG ACAGGCCAGC AUCAGUUUUG GUGGCAGGAU AAAUCCAUAG GAAUG UAGC UUGCCUCGGU AAGUAUUAUA GCCUGUGGGA AUACUGCCAG CUGGGACUGA GGACUGCGAC GUAAGUCAAG GAUGCU GGC AUAAUGGUUA UAUGCCGCCC GUCUUGAAAC ACGGACCAAG GAGUCUAACG UCUAUGCGAG UGUUUGGGUG UAAAACC CA UACGCGUAAU GAAAGUGAAC GUAGGUUGGG GCCUCGCAAG AGGUGCACAA UCGACCGAUC CUGAUGUCUU CGGAUGGA U UUGAGUAAGA GCAUAGCUGU UGGGACCCGA AAGAUGGUGA ACUAUGCCUG AAUAGGGUGA AGCCAGAGGA AACUCUGGU GGAGGCUCGU AGCGGUUCUG ACGUGCAAAU CGAUCGUCGA AUUUGGGUAU AGGGGCGAAA GACUAAUCGA ACCAUCUAGU AGCUGGUUC CUGCCGAAGU UUCCCUCAGG AUAGCAGAAG CUCGUAUCAG UUUUAUGAGG UAAAGCGAAU GAUUAGAGGU U CCGGGGUC GAAAUGACCU UGACCUAUUC UCAAACUUUA AAUAUGUAAG AAGUCCUUGU UACUUAAUUG AACGUGGACA UU UGAAUGA AGAGCUUUUA GUGGGCCAUU UUUGGUAAGC AGAACUGGCG AUGCGGGAUG AACCGAACGU AGAGUUAAGG UGC CGGAAU ACACGCUCAU CAGACACCAC AAAAGGUGUU AGUUCAUCUA GACAGCCGGA CGGUGGCCAU GGAAGUCGGA AUCC GCUAA GGAGUGUGUA ACAACUCACC GGCCGAAUGA ACUAGCCCUG AAAAUGGAUG GCGCUCAAGC GUGUUACCUA UACUC UACC GUCAGGGUUG AUAUGAUGCC CUGACGAGUA GGCAGGCGUG GAGGUCAGUG ACGAAGCCUA GACCGUAAGG UCGGGU CGA ACGGCCUCUA GUGCAGAUCU UGGUGGUAGU AGCAAAUAUU CAAAUGAGAA CUUUGAAGAC UGAAGUGGGG AAAGGUU CC ACGUCAACAG CAGUUGGACG UGGGUUAGUC GAUCCUAAGA GAUGGGGAAG CUCCGUUUCA AAGGCCUGAU UUUAUGCA G GCCACCAUCG AAAGGGAAUC CGGUUAAGAU UCCGGAACCU GGAUAUGGAU UCUUCACGGU AACGUAACUG AAUGUGGAG ACGUCGGCGC GAGCCCUGGG AGGAGUUAUC UUUUCUUCUU AACAGCUUAU CACCCCGGAA UUGGUUUAUC CGGAGAUGGG GUCUUAUGG CUGGAAGAGG CCAGCACCUU UGCUGGCUCC GGUGCGCUUG UGACGGCCCG UGAAAAUCCA CAGGAAGGAA U AGUUUUCA UGCCAGGUCG UACUGAUAAC CGCAGCAGGU CUCCAAGGUG AACAGCCUCU AGUUGAUAGA AUAAUGUAGA UA AGGGAAG UCGGCAAAAU AGAUCCGUAA CUUCGGGAUA AGGAUUGGCU CUAAGGGUCG ACGAUCAACU UAGAACUGGU ACG GACAAG GGGAAUCUGA CUGUCUAAUU AAAACAUAGC AUUGCGAUGG UCAGAAAGUG AUGUUGACGC AAUGUGAUUU CUGC CCAGU GCUCUGAAUG UCAAAGUGAA GAAAUUCAAC CAAGCGCGGG UAAACGGCGG GAGUAACUAU GACUCUCUUA AGGUA GCCA AAUGCCUCGU CAUCUAAUUA GUGACGCGCA UGAAUGGAUU AACGAGAUUC CCACUGUCCC UAUCUACUAU CUAGCG AAA CCACAGCCAA GGGAACGGGC UUGGCAGAAU CAGCGGGGAA AGAAGACCCU GUUGAGCUUG ACUCUAGUUU GACAUUG UG AAGAGACAUA GAGGGCUACC UUUAUAGUUU CUUUACUUAU UCAAUGAAGC GGAGCUGGAA UUCAUUUUCC ACGUUCUA G CAUUCAAGGU CCCAUUCGGG GCUGAUCCGG GUUGAAGACA UUGUCAGGUG GGGAGUUUGG CUGGGGCGGC ACAUCUGUU AAACGAUAAC GCAGAUGUCC UAAGGGGGGC UCAUGGAGAA CAGAAAUCUC CAGUAGAACA AAAGGGUAAA AGCCCCCUUG AUUUUGAUU UUCAGUGUGA AUACAAACCA UGAAAGUGUG GCCUAUCGAU CCUUUAGUCC CUCGGAAUUU GAGGCUAGAG G UGCCAGAA AAGUUACCAC AGGGAUAACU GGCUUGUGGC AGUCAAGCGU UCAUAGCGAC AUUGCUUUUU GAUUCUUCGA UG UCGGCUC UUCCUAUCAU ACCGAAGCAG AAUUCGGUAA GCGUUGGAUU GUUCACCCAC UAAUAGGGAA CGUGAGCUGG GUU UAGACC GUCGUGAGAC AGGUUAGUUU UACCCUACUG AUGAAUGUUA CCGCAAUAGU AAUUGAACUU AGUACGAGAG GAAC AGUUC AUUCGGAUAA UUGGUUUUUG CGGCUGUCUG AUCAGGCAUU GCCGCGAAGC UACCAUCCGC UGGAUUAUGG CUGAA CGCC UCUAAGUCAG AAUCCAUGCU AGAACGCGGU GAUUUCUUUG CUCCACACAA UAUAGAUGGA UACGAAUAAG GCGUCC UUG UGGCGUCGCU GAACCAUAGC AGGCUAGCAA CGGUGCACUU GGCGGAAAGG CCUUGGGUGC UUGCUGGCGA AUUGCAA UG UCAUUUUGCG UGGGGAUAAA UCAUUUGUAU ACGACUUAGA UGUACAACGG GGUAUUGUAA GCAGUAGAGU AGCCUUGU U GUUACGAUCU GCUGAGAUUA AGCCUUUGUU GUCUGAUUUG U

Macromolecule #14: 18S ribosomal RNA

• Macromolecule: Name: 18S ribosomal RNA / type: rna / ID: 14 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 559.592188 KDa

Sequence

String:

UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGUAUAAC UGUGGUAAUU C UAGAGCUA AUACAUGCUU AAAAUCUCGA CCCUUUGGAA GAGAUGUAUU UAUUAGAUAA AAAAUCAAUG UCUUCGGACU CU UUGAUGA UUCAUAAUAA CUUUUCGAAU CGCAUGGCCU UGUGCUGGCG AUGGUUCAUU CAAAUUUCUG CCCUAUCAAC UUU CGAUGG UAGGAUAGUG GCCUACCAUG GUUUCAACGG GUAACGGGGA AUAAGGGUUC GAUUCCGGAG AGGGAGCCUG AGAA ACGGC UACCACAUCC AAGGAAGGCA GCAGGCGCGC AAAUUACCCA AUCCUAAUUC AGGGAGGUAG UGACAAUAAA UAACG AUAC AGGGCCCAUU CGGGUCUUGU AAUUGGAAUG AGUACAAUGU AAAUACCUUA ACGAGGAACA AUUGGAGGGC AAGUCU GGU GCCAGCAGCC GCGGUAAUUC CAGCUCCAAU AGCGUAUAUU AAAGUUGUUG CAGUUAAAAA GCUCGUAGUU GAACUUU GG GCCCGGUUGG CCGGUCGGAU UUCCAACGGG GCCUUUCCUU CUGGCUAACC UUGAGUCCUU GUGGCUCUUG GCGAACCA G GACUUUUACU UUGAAAAAAU UAGAGUGUUC AAAGCAGGCG UAUUGCUCGA AUAUAUUAGC AUGGAAUAAU AGAAUAGGA CGUUUGGUUC UAUUUUGUUG GUUUCUAGGA CCAUCGUAAU GAUUAAUAGG GACGGUCGGG GGCAUCAGUA UUCAAUUGUC AGAGGUGAA AUUCUUGGAU UUAUUGAAGA CUAACUACUG CGAAAGCAUU UGCCAAGGAC GUUUUCAUUA AUCAAGAACG A AAGUUAGG GGAUCGAAGA UGAUCAGAUA CCGUCGUAGU CUUAACCAUA AACUAUGCCG ACUAGGGAUC GGGUGCUUGC AC UCGGCAC CUUACGAGAA AUCAAAGUCU UUGGGUUCUG GGGGGAGUAU GGUCGCAAGG CUGAAACUUA AAGGAAUUGA CGG AAGGGC ACCACCAGGA GUGGAGCCUG CGGCUUAAUU UGACUCAACA CGGGGAAACU CACCAGGUCC AGACACAAUA AGGA UUGAC AGAUUGAGAG CUCUUUCUUG AUUUUGUGGG UGGUGGUGCA UGGCCGUUCU UAGUUGGUGG AGUGAUUUGU CUGCU UAAU UGCGAUAACG AACGAGACCU UAACCUACUA AAUAGUGGUG CUAGCAUUUG CUGGUUAUCC ACUUCUUAGA GGGACU AUC GGUUUCAAGC CGAUGGAAGU UUGAGGCAAU AACAGGUCUG UGAUGCCCUU AGACGUUCUG GGCCGCACGC GCGCUAC AC UGACGGAGCC AGCGAGUCUA ACCUUGGCCG AGAGGUCUUG GUAAUCUUGU GAAACUCCGU CGUGCUGGGG AUAGAGCA U UGUAAUUAUU GCUCUUCAAC GAGGAAUUCC UAGUAAGCGC AAGUCAUCAG CUUGCGUUGA UUACGUCCCU GCCCUUUGU ACACACCGCC CGUCGCUAGU ACCGAUUGAA UGGCUUAGUG AGGCCUCAGG AUCUGGGAGA AUUUGGACAA ACUUGGUCAU UUAGAGGAA CUAAAAGUCG UAACAAGGUU UCCGUAGGUG AACCUGCGGA AGGAUCAU

Macromolecule #82: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 82 / Number of copies: 220 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #83: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 83 / Number of copies: 72 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Macromolecule #84: 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethy...

• Macromolecule: Name: 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione; type: ligand / ID: 84 / Number of copies: 1 / Formula: 3HE
• Molecular weight: Theoretical: 281.347 Da

Chemical component information

ChemComp-3HE:
4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione / Cycloheximide

Macromolecule #85: SPERMIDINE

• Macromolecule: Name: SPERMIDINE / type: ligand / ID: 85 / Number of copies: 3 / Formula: SPD
• Molecular weight: Theoretical: 145.246 Da

Chemical component information

ChemComp-SPD:
SPERMIDINE / Spermidine

Macromolecule #86: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 86 / Number of copies: 7 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #87: water

• Macromolecule: Name: water / type: ligand / ID: 87 / Number of copies: 1810 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER / Water

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.5
• Grid: Model: Quantifoil / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Support film - Film thickness: 300 / Pretreatment - Type: GLOW DISCHARGE
• Vitrification: Cryogen name: ETHANE-PROPANE
• Details: 80S peak from Sucrose gradient

Electron microscopy

• Microscope: FEI TALOS ARCTICA
• Electron beam: Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.6 µm
• Image recording: Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Detector mode: COUNTING / Average electron dose: 32.0 e/Ų
• Experimental equipment: Model: Talos Arctica / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 254202

Startup model

Type of model: PDB ENTRY
PDB model - PDB ID:

4u50

Details: Crystal structure of Verrucarin bound to the yeast 80S ribosome

• Initial angle assignment: Type: ANGULAR RECONSTITUTION
• Final 3D classification: Number classes: 5 / Software - Name: RELION (ver. 4.1)
• Final angle assignment: Type: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 4.1)
• Final reconstruction: Number classes used: 2 / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 2.4 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 4.1) / Number images used: 127945

Atomic model buiding 1

• Refinement: Protocol: RIGID BODY FIT / Target criteria: Correlation coefficient

Output model

PDB-8bn3:
Yeast 80S, ES7s delta, eIF5A, Stm1 containing