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Yorodumi- EMDB-15452: Cryo-EM structure of the nitrogen-fixation associated NADH:ferred... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-15452 | |||||||||||||||
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Title | Cryo-EM structure of the nitrogen-fixation associated NADH:ferredoxin oxidoreductase RNF from Azotobacter vinelandii | |||||||||||||||
Map data | unsharpened map | |||||||||||||||
Sample |
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Keywords | metalloprotein / flavoprotein / electron transfer and Na+/H+ translocation / MEMBRANE PROTEIN | |||||||||||||||
Function / homology | Function and homology information Translocases / prenyltransferase activity / metabolic process / electron transport chain / transmembrane transport / FMN binding / 4 iron, 4 sulfur cluster binding / electron transfer activity / metal ion binding / plasma membrane Similarity search - Function | |||||||||||||||
Biological species | Azotobacter vinelandii DJ (bacteria) | |||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.11 Å | |||||||||||||||
Authors | Zhang L / Einsle O | |||||||||||||||
Funding support | European Union, Germany, 4 items
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Citation | Journal: To Be Published Title: Architecture of the NADH:ferredoxin oxidoreductase RNF that drives Biological Nitrogen Fixation Authors: Zhang L / Einsle O | |||||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_15452.map.gz | 75.5 MB | EMDB map data format | |
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Header (meta data) | emd-15452-v30.xml emd-15452.xml | 25.6 KB 25.6 KB | Display Display | EMDB header |
Images | emd_15452.png | 151.9 KB | ||
Filedesc metadata | emd-15452.cif.gz | 7 KB | ||
Others | emd_15452_additional_1.map.gz emd_15452_half_map_1.map.gz emd_15452_half_map_2.map.gz | 78.1 MB 77.8 MB 77.8 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-15452 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-15452 | HTTPS FTP |
-Related structure data
Related structure data | 8ahxMC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_15452.map.gz / Format: CCP4 / Size: 83.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | unsharpened map | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.82 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: sharpened map
File | emd_15452_additional_1.map | ||||||||||||
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Annotation | sharpened map | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: halp map A
File | emd_15452_half_map_1.map | ||||||||||||
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Annotation | halp map A | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: halp map B
File | emd_15452_half_map_2.map | ||||||||||||
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Annotation | halp map B | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
+Entire : Heptameric complex of NADH:ferredoxin oxidoreductase RNF
+Supramolecule #1: Heptameric complex of NADH:ferredoxin oxidoreductase RNF
+Macromolecule #1: Ion-translocating oxidoreductase complex subunit A
+Macromolecule #2: Ion-translocating oxidoreductase complex subunit B
+Macromolecule #3: Ion-translocating oxidoreductase complex subunit C
+Macromolecule #4: Ion-translocating oxidoreductase complex subunit D
+Macromolecule #5: Ion-translocating oxidoreductase complex subunit E
+Macromolecule #6: Ion-translocating oxidoreductase complex subunit G
+Macromolecule #7: Protein RnfH
+Macromolecule #8: FE2/S2 (INORGANIC) CLUSTER
+Macromolecule #9: DODECYL-BETA-D-MALTOSIDE
+Macromolecule #10: IRON/SULFUR CLUSTER
+Macromolecule #11: FLAVIN MONONUCLEOTIDE
+Macromolecule #12: RIBOFLAVIN
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | cell |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 47.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: INSILICO MODEL |
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Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: ANGULAR RECONSTITUTION |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.11 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 94807 |