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- EMDB-13479: Structural basis of Integrator-mediated transcription regulation -

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Basic information

Entry
Database: EMDB / ID: EMD-13479
TitleStructural basis of Integrator-mediated transcription regulation
Map data
Sample
  • Complex: PEC-Integrator-PP2A complex
    • Complex: Pol II-EC
      • Protein or peptide: x 13 types
      • DNA: x 2 types
      • RNA: x 1 types
    • Complex: human Integrator-PP2A complex
      • Protein or peptide: x 12 types
    • Complex: NELF
      • Protein or peptide: x 4 types
    • Complex: DSIF
      • Protein or peptide: x 1 types
    • Complex: Non-template DNA
    • Complex: RNA
    • Complex: Template DNA
  • Ligand: x 3 types
Function / homology
Function and homology information


U2 snRNA 3'-end processing / NELF complex / positive regulation of protein modification process / snRNA processing / integrator complex / snRNA 3'-end processing / meiotic spindle elongation / Integration of energy metabolism / PP2A-mediated dephosphorylation of key metabolic factors / regulation of microtubule binding ...U2 snRNA 3'-end processing / NELF complex / positive regulation of protein modification process / snRNA processing / integrator complex / snRNA 3'-end processing / meiotic spindle elongation / Integration of energy metabolism / PP2A-mediated dephosphorylation of key metabolic factors / regulation of microtubule binding / NTRK3 as a dependence receptor / negative regulation of DNA-templated transcription, elongation / MASTL Facilitates Mitotic Progression / mitotic sister chromatid separation / regulation of meiotic cell cycle process involved in oocyte maturation / protein phosphatase type 2A complex / meiotic sister chromatid cohesion, centromeric / peptidyl-serine dephosphorylation / DSIF complex / regulation of transcription elongation by RNA polymerase II / peptidyl-threonine dephosphorylation / : / positive regulation of microtubule binding / negative regulation of tyrosine phosphorylation of STAT protein / Regulation of glycolysis by fructose 2,6-bisphosphate metabolism / Inhibition of replication initiation of damaged DNA by RB1/E2F1 / female meiotic nuclear division / protein antigen binding / protein phosphatase regulator activity / ceramide metabolic process / GABA receptor binding / negative regulation of epithelial to mesenchymal transition / APC truncation mutants have impaired AXIN binding / AXIN missense mutants destabilize the destruction complex / Truncations of AMER1 destabilize the destruction complex / B-WICH complex positively regulates rRNA expression / RNA Polymerase I Transcription Initiation / RNA Polymerase I Promoter Escape / RNA Polymerase I Transcription Termination / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / Initiation of Nuclear Envelope (NE) Reformation / Formation of RNA Pol II elongation complex / Formation of the Early Elongation Complex / Transcriptional regulation by small RNAs / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / FGFR2 alternative splicing / RNA polymerase II transcribes snRNA genes / mRNA Capping / mRNA Splicing - Major Pathway / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Elongation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Pol II CTD phosphorylation and interaction with CE / Estrogen-dependent gene expression / ERKs are inactivated / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / negative regulation of stem cell differentiation / positive regulation of extrinsic apoptotic signaling pathway in absence of ligand / Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters / nuclear lumen / Beta-catenin phosphorylation cascade / Signaling by GSK3beta mutants / CTNNB1 S33 mutants aren't phosphorylated / CTNNB1 S37 mutants aren't phosphorylated / CTNNB1 S45 mutants aren't phosphorylated / CTNNB1 T41 mutants aren't phosphorylated / : / Abortive elongation of HIV-1 transcript in the absence of Tat / regulation of Wnt signaling pathway / positive regulation of DNA-templated transcription, elongation / Disassembly of the destruction complex and recruitment of AXIN to the membrane / regulation of growth / : / transcription elongation-coupled chromatin remodeling / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / inner cell mass cell proliferation / negative regulation of glycolytic process through fructose-6-phosphate / positive regulation of NLRP3 inflammasome complex assembly / myosin phosphatase activity / protein serine/threonine phosphatase activity / CTLA4 inhibitory signaling / RNA Pol II CTD phosphorylation and interaction with CE during HIV infection / RNA Pol II CTD phosphorylation and interaction with CE / Platelet sensitization by LDL / Formation of the Early Elongation Complex / Formation of the HIV-1 Early Elongation Complex / mRNA Capping / negative regulation of MAPK cascade / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / protein-serine/threonine phosphatase / regulation of cell differentiation
Similarity search - Function
Domain of unknown function DUF3677 / Integrator complex subunit 2, metazoa / Integrator complex subunit 2 / Integrator complex subunit 5, C-terminal / Integrator complex subunit 5, N-terminal / Integrator complex subunit 7 / Integrator complex subunit 8 / Integrator complex subunit 1 / Integrator complex subunit 5 / Protein of unknown function (DUF3677) ...Domain of unknown function DUF3677 / Integrator complex subunit 2, metazoa / Integrator complex subunit 2 / Integrator complex subunit 5, C-terminal / Integrator complex subunit 5, N-terminal / Integrator complex subunit 7 / Integrator complex subunit 8 / Integrator complex subunit 1 / Integrator complex subunit 5 / Protein of unknown function (DUF3677) / Integrator complex subunit 2 / Integrator complex subunit 5 N-terminus / Integrator complex subunit 5 C-terminus / INTS6/SAGE1/DDX26B/CT45, C-terminal / INTS6/SAGE1/DDX26B/CT45 C-terminus / Cofactor of BRCA1 / Cofactor of BRCA1 (COBRA1) / : / : / Integrator IntS9, C-terminal domain / Integrator IntS11, C-terminal domain / TH1 protein / Integrator complex subunit 9 / Integrator complex subunit 11, MBL-fold / TH1 protein / Sister chromatid cohesion protein PDS5 protein / Hepatitis delta antigen (HDAg) domain / Hepatitis delta antigen (HDAg) domain profile. / Metallo-beta-lactamase superfamily domain / : / Beta-Casp domain / Beta-Casp domain / Beta-Casp domain / Spt5, KOW domain repeat 6 / Zn-dependent metallo-hydrolase, RNA specificity domain / Zn-dependent metallo-hydrolase RNA specificity domain / HEAT repeat / Spt5 C-terminal domain / HEAT repeat / Spt5 C-terminal nonapeptide repeat binding Spt4 / Transcription elongation factor Spt5, eukaryote / Spt5 transcription elongation factor, N-terminal / Spt5, KOW domain repeat 2 / Spt5, KOW domain repeat 3 / Spt5, KOW domain repeat 5 / Spt5 transcription elongation factor, acidic N-terminal / NGN domain, eukaryotic / Spt5, KOW domain repeat 1 / Spt5, KOW domain repeat 4 / NGN domain / Transcription elongation factor SPT5 / Early transcription elongation factor of RNA pol II, NGN section / Serine/threonine specific protein phosphatases signature. / Protein phosphatase 2A homologues, catalytic domain. / RNA-binding domain, S1 / Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase / HEAT repeat profile. / HEAT, type 2 / von Willebrand factor type A domain / HEAT repeats / NusG, N-terminal / In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold. / NusG, N-terminal domain superfamily / Calcineurin-like phosphoesterase domain, ApaH type / Calcineurin-like phosphoesterase / DNA-directed RNA polymerase II subunit Rpb4-like / RNA polymerase Rpb4/RPC9, core / DNA-directed RNA-polymerase II subunit / RNA polymerase II, heptapeptide repeat, eukaryotic / RNA polymerase Rpb1, domain 6 / Metallo-dependent phosphatase-like / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb1 C-terminal repeat / Eukaryotic RNA polymerase II heptapeptide repeat. / RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb1, domain 7 superfamily / RNA polymerase Rpb1, domain 7 / Rpb4/RPC9 superfamily / Pol II subunit B9, C-terminal zinc ribbon / RNA polymerase RBP11 / RNA polymerase subunit Rpb4/RPC9 / RNA polymerase Rpb4 / Zinc finger TFIIS-type signature. / RNA polymerase subunit Rpb7-like / RNA polymerase Rpb7-like , N-terminal / RNA polymerase Rpb7-like, N-terminal domain superfamily / SHS2 domain found in N terminus of Rpb7p/Rpc25p/MJ0397 / HRDC-like superfamily / RNA polymerase Rpb2, domain 4 / RNA polymerase Rpb2, domain 4 / RNA polymerase Rpb2, domain 5 / RNA polymerase Rpb2, domain 5 / DNA-directed RNA polymerase, M/15kDa subunit / RNA polymerases M/15 Kd subunit / RNA polymerase subunit 9 / DNA-directed RNA polymerase subunit RPABC5/Rpb10 / RNA polymerases, subunit N, zinc binding site / RNA polymerase subunit RPB10 / RNA polymerases N / 8 kDa subunit / RNA polymerases N / 8 Kd subunits signature.
Similarity search - Domain/homology
DNA-directed RNA polymerase subunit beta / RNA polymerase II subunit D / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerase II subunit RPB11-a / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerase subunit / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerase subunit / DNA-directed RNA polymerase II subunit RPB7 ...DNA-directed RNA polymerase subunit beta / RNA polymerase II subunit D / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerase II subunit RPB11-a / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerase subunit / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerase subunit / DNA-directed RNA polymerase II subunit RPB7 / RNA polymerase II, I and III subunit K / DNA-directed RNA polymerase II subunit E / Transcription elongation factor SPT5 / Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform / DNA-directed RNA polymerase II subunit RPB9 / Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform / Integrator complex subunit 11 / Integrator complex subunit 5 / Integrator complex subunit 8 / Negative elongation factor C/D / Integrator complex subunit 1 / Negative elongation factor B / Integrator complex subunit 4 / Integrator complex subunit 2 / Negative elongation factor A / Integrator complex subunit 9 / Integrator complex subunit 7 / Integrator complex subunit 6
Similarity search - Component
Biological speciesSus scrofa domesticus (domestic pig) / Homo sapiens (human) / Human immunodeficiency virus 1 / domestic pig (domestic pig)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsFianu I / Chen Y / Dienemann C / Cramer P
Funding supportEuropean Union, 2 items
OrganizationGrant numberCountry
German Research Foundation (DFG)EXC 2067/1-390729940European Union
European Research Council (ERC)882357European Union
CitationJournal: Science / Year: 2021
Title: Structural basis of Integrator-mediated transcription regulation.
Authors: Isaac Fianu / Ying Chen / Christian Dienemann / Olexandr Dybkov / Andreas Linden / Henning Urlaub / Patrick Cramer /
Abstract: Integrator and protein phosphatase 2A (PP2A) form a complex that dephosphorylates paused RNA polymerase II (Pol II), cleaves the nascent RNA, and terminates transcription. We report the structure of ...Integrator and protein phosphatase 2A (PP2A) form a complex that dephosphorylates paused RNA polymerase II (Pol II), cleaves the nascent RNA, and terminates transcription. We report the structure of the pretermination complex containing the human Integrator-PP2A complex bound to paused Pol II. Integrator binds Pol II and the pausing factors DSIF and NELF to exclude binding of the elongation factors SPT6 and PAF1 complex. Integrator also binds the C-terminal domain of Pol II and positions PP2A to counteract Pol II phosphorylation and elongation. The Integrator endonuclease docks to the RNA exit site and opens to cleave nascent RNA about 20 nucleotides from the Pol II active site. Integrator does not bind the DNA clamps formed by Pol II and DSIF, enabling release of DNA and transcription termination.
History
DepositionAug 26, 2021-
Header (metadata) releaseDec 8, 2021-
Map releaseDec 8, 2021-
UpdateDec 8, 2021-
Current statusDec 8, 2021Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7pks
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_13479.png

Downloads & links

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Map

FileDownload / File: emd_13479.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.05 Å
Density
Contour LevelBy AUTHOR: 0.025 / Movie #1: 0.025
Minimum - Maximum-0.056292966 - 0.20347115
Average (Standard dev.)0.001005423 (±0.006069143)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 503.99997 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.051.051.05
M x/y/z480480480
origin x/y/z0.0000.0000.000
length x/y/z504.000504.000504.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS480480480
D min/max/mean-0.0560.2030.001

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Supplemental data

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Mask #1

Fileemd_13479_msk_1.map
Projections & Slices
AxesZYX

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Additional map: #1

Fileemd_13479_additional_1.map
Projections & Slices
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Half map: #2

Fileemd_13479_half_map_1.map
Projections & Slices
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Half map: #1

Fileemd_13479_half_map_2.map
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Sample components

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Entire : PEC-Integrator-PP2A complex

EntireName: PEC-Integrator-PP2A complex
Components
  • Complex: PEC-Integrator-PP2A complex
    • Complex: Pol II-EC
      • Protein or peptide: DNA-directed RNA polymerase subunitPolymerase
      • Protein or peptide: DNA-directed RNA polymerase subunit betaPolymerase
      • Protein or peptide: DNA-directed RNA polymerase II subunit RPB3Polymerase
      • Protein or peptide: RNA polymerase II subunit D
      • Protein or peptide: DNA-directed RNA polymerase II subunit EPolymerase
      • Protein or peptide: DNA-directed RNA polymerase II subunit FPolymerase
      • Protein or peptide: DNA-directed RNA polymerase II subunit RPB7Polymerase
      • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC3RNA polymerase
      • Protein or peptide: DNA-directed RNA polymerase II subunit RPB9Polymerase
      • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC5RNA polymerase
      • Protein or peptide: RNA_pol_L_2 domain-containing protein
      • Protein or peptide: RNA polymerase II subunit K
      • Protein or peptide: RPBI C-terminal domain peptide
      • DNA: Non-template DNA
      • RNA: TAR RNA
      • DNA: DNA Template
    • Complex: human Integrator-PP2A complex
      • Protein or peptide: Integrator complex subunit 1
      • Protein or peptide: Integrator complex subunit 2
      • Protein or peptide: Integrator complex subunit 4
      • Protein or peptide: Integrator complex subunit 5
      • Protein or peptide: Integrator complex subunit 6
      • Protein or peptide: Integrator complex subunit 7
      • Protein or peptide: Integrator complex subunit 8
      • Protein or peptide: Integrator complex subunit 9
      • Protein or peptide: Integrator complex subunit 11
      • Protein or peptide: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
      • Protein or peptide: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
      • Protein or peptide: Unknown
    • Complex: NELF
      • Protein or peptide: Negative elongation factor A
      • Protein or peptide: Negative elongation factor B
      • Protein or peptide: Negative elongation factor C/D
      • Protein or peptide: Negative elongation factor E
    • Complex: DSIF
      • Protein or peptide: Transcription elongation factor SPT5
    • Complex: Non-template DNA
    • Complex: RNA
    • Complex: Template DNA
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: MANGANESE (II) ION

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Supramolecule #1: PEC-Integrator-PP2A complex

SupramoleculeName: PEC-Integrator-PP2A complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#33
Molecular weightTheoretical: 1.5 MDa

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Supramolecule #2: Pol II-EC

SupramoleculeName: Pol II-EC / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#16
Source (natural)Organism: Sus scrofa domesticus (domestic pig)

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Supramolecule #3: human Integrator-PP2A complex

SupramoleculeName: human Integrator-PP2A complex / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #22-#33
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)

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Supramolecule #4: NELF

SupramoleculeName: NELF / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #17-#20
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)

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Supramolecule #5: DSIF

SupramoleculeName: DSIF / type: complex / ID: 5 / Parent: 1 / Macromolecule list: #21
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)

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Supramolecule #6: Non-template DNA

SupramoleculeName: Non-template DNA / type: complex / ID: 6 / Parent: 1 / Macromolecule list: #14
Source (natural)Organism: Human immunodeficiency virus 1

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Supramolecule #7: RNA

SupramoleculeName: RNA / type: complex / ID: 7 / Parent: 1 / Macromolecule list: #15
Source (natural)Organism: Human immunodeficiency virus 1
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)

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Supramolecule #8: Template DNA

SupramoleculeName: Template DNA / type: complex / ID: 8 / Parent: 1 / Macromolecule list: #16
Source (natural)Organism: Human immunodeficiency virus 1
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)

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Macromolecule #1: DNA-directed RNA polymerase subunit

MacromoleculeName: DNA-directed RNA polymerase subunit / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: domestic pig (domestic pig)
Molecular weightTheoretical: 217.450078 KDa
SequenceString: MHGGGPPSGD SACPLRTIKR VQFGVLSPDE LKRMSVTEGG IKYPETTEGG RPKLGGLMDP RQGVIERTGR CQTCAGNMTE CPGHFGHIE LAKPVFHVGF LVKTMKVLRC VCFFCSKLLV DSNNPKIKDI LAKSKGQPKK RLTHVYDLCK GKNICEGGEE M DNKFGVEQ ...String:
MHGGGPPSGD SACPLRTIKR VQFGVLSPDE LKRMSVTEGG IKYPETTEGG RPKLGGLMDP RQGVIERTGR CQTCAGNMTE CPGHFGHIE LAKPVFHVGF LVKTMKVLRC VCFFCSKLLV DSNNPKIKDI LAKSKGQPKK RLTHVYDLCK GKNICEGGEE M DNKFGVEQ PEGDEDLTKE KGHGGCGRYQ PRIRRSGLEL YAEWKHVNED SQEKKILLSP ERVHEIFKRI SDEECFVLGM EP RYARPEW MIVTVLPVPP LSVRPAVVMQ GSARNQDDLT HKLADIVKIN NQLRRNEQNG AAAHVIAEDV KLLQFHVATM VDN ELPGLP RAMQKSGRPL KSLKQRLKGK EGRVRGNLMG KRVDFSARTV ITPDPNLSID QVGVPRSIAA NMTFAEIVTP FNID RLQEL VRRGNSQYPG AKYIIRDNGD RIDLRFHPKP SDLHLQTGYK VERHMCDGDI VIFNRQPTLH KMSMMGHRVR ILPWS TFRL NLSVTTPYNA DFDGDEMNLH LPQSLETRAE IQELAMVPRM IVTPQSNRPV MGIVQDTLTA VRKFTKRDVF LERGEV MNL LMFLSTWDGK VPQPAILKPR PLWTGKQIFS LIIPGHINCI RTHSTHPDDE DSGPYKHISP GDTKVVVENG ELIMGIL CK KSLGTSAGSL VHISYLEMGH DITRLFYSNI QTVINNWLLI EGHTIGIGDS IADSKTYQDI QNTIKKAKQD VIEVIEKA H NNELEPTPGN TLRQTFENQV NRILNDARDK TGSSAQKSLS EYNNFKSMVV SGAKGSKINI SQVIAVVGQQ NVEGKRIPF GFKHRTLPHF IKDDYGPESR GFVENSYLAG LTPTEFFFHA MGGREGLIDT AVKTAETGYI QRRLIKSMES VMVKYDATVR NSINQVVQL RYGEDGLAGE SVEFQNLATL KPSNKAFEKK FRFDYTNERA LRRTLQEDLV KDVLSNAHIQ NELEREFERM R EDREVLRV IFPTGDSKVV LPCNLLRMIW NAQKIFHINP RLPSDLHPIK VVEGVKELSK KLVIVNGDDP LSRQAQENAT LL FNIHLRS TLCSRRMAEE FRLSGEAFDW LLGEIESKFN QAIAHPGEMV GALAAQSLGE PATQMTLNTF HYAGVSAKNV TLG VPRLKE LINISKKPKT PSLTVFLLGQ SARDAERAKD ILCRLEHTTL RKVTANTAIY YDPNPQSTVV AEDQEWVNVY YEMP DFDVA RISPWLLRVE LDRKHMTDRK LTMEQIAEKI NAGFGDDLNC IFNDDNAEKL VLRIRIMNSD ENKMQEEEEV VDKMD DDVF LRCIESNMLT DMTLQGIEQI SKVYMHLPQT DNKKKIIITE DGEFKALQEW ILETDGVSLM RVLSEKDVDP VRTTSN DIV EIFTVLGIEA VRKALERELY HVISFDGSYV NYRHLALLCD TMTCRGHLMA ITRHGVNRQD TGPLMKCSFE ETVDVLM EA AAHGESDPMK GVSENIMLGQ LAPAGTGCFD LLLDAEKCKY GMEIPTNIPG LGAAGPTGMF FGSAPSPMGG ISPAMTPW N QGATPAYGAW SPSVGSGMTP GAAGFSPSAA SDASGFSPGY SPAWSPTPGS PGSPGPSSPY IPSPGGAMSP SYSPTSPAY EPRSPGGYTP QSPSYSPTSP SYSPTSPSYS PTSPNYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPS YSPTSPSYSP TSPSYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPS YSPTSPSYSP TSPSYSPTSP SYSPTSPNYS PTSPNYTPTS P SYSPTSPS YSPTSPNYTP TSPNYSPTSP SYSPTSPSYS PTSPSYSPSS PRYTPQSPTY TPSSPSYSPS SPSYSPTSPK YT PTSPSYS PSSPEYTPTS PKYSPTSPKY SPTSPKYSPT SPTYSPTTPK YSPTSPTYSP TSPVYTPTSP KYSPTSPTYS PTS PKYSPT SPTYSPTSPK GSTYSPTSPG YSPTSPTYSL TSPAISPDDS DEEN

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Macromolecule #2: DNA-directed RNA polymerase subunit beta

MacromoleculeName: DNA-directed RNA polymerase subunit beta / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: domestic pig (domestic pig) / Tissue: Thymus
Molecular weightTheoretical: 134.041422 KDa
SequenceString: MYDADEDMQY DEDDDEITPD LWQEACWIVI SSYFDEKGLV RQQLDSFDEF IQMSVQRIVE DAPPIDLQAE AQHASGEVEE PPRYLLKFE QIYLSKPTHW ERDGAPSPMM PNEARLRNLT YSAPLYVDIT KTVIKEGEEQ LQTQHQKTFI GKIPIMLRST Y CLLNGLTD ...String:
MYDADEDMQY DEDDDEITPD LWQEACWIVI SSYFDEKGLV RQQLDSFDEF IQMSVQRIVE DAPPIDLQAE AQHASGEVEE PPRYLLKFE QIYLSKPTHW ERDGAPSPMM PNEARLRNLT YSAPLYVDIT KTVIKEGEEQ LQTQHQKTFI GKIPIMLRST Y CLLNGLTD RDLCELNECP LDPGGYFIIN GSEKVLIAQE KMATNTVYVF AKKDSKYAYT GECRSCLENS SRPTSTIWVS ML ARGGQGA KKSAIGQRIV ATLPYIKQEV PIIIVFRALG FVSDRDILEH IIYDFEDPEM MEMVKPSLDE AFVIQEQNVA LNF IGSRGA KPGVTKEKRI KYAKEVLQKE MLPHVGVSDF CETKKAYFLG YMVHRLLLAA LGRRELDDRD HYGNKRLDLA GPLL AFLFR GMFKNLLKEV RIYAQKFIDR GKDFNLELAI KTRIISDGLK YSLATGNWGD QKKAHQARAG VSQVLNRLTF ASTLS HLRR LNSPIGRDGK LAKPRQLHNT LWGMVCPAET PEGHAVGLVK NLALMAYISV GSQPSPILEF LEEWSMENLE EISPAA IAD ATKIFVNGCW VGIHKDPEQL MNTLRKLRRQ MDIIVSEVSM IRDIREREIR IYTDAGRICR PLLIVEKQKL LLKKRHI DQ LKEREYNNYS WQDLVASGVV EYIDTLEEET VMLAMTPDDL QEKEVAYCST YTHCEIHPSM ILGVCASIIP FPDHNQSP R NTYQSAMGKQ AMGVYITNFH VRMDTLAHVL YYPQKPLVTT RSMEYLRFRE LPAGINSIVA IASYTGYNQE DSVIMNRSA VDRGFFRSVF YRSYKEQESK KGFDQEEVFE KPTRETCQGM RHAIYDKLDD DGLIAPGVRV SGDDVIIGKT VTLPENEDEL EGTNRRYTK RDCSTFLRTS ETGIVDQVMV TLNQEGYKFC KIRVRSVRIP QIGDKFASRH GQKGTCGIQY RQEDMPFTCE G ITPDIIIN PHAIPSRMTI GHLIECLQGK VSANKGEIGD ATPFNDAVNV QKISNLLSDY GYHLRGNEVL YNGFTGRKIT SQ IFIGPTY YQRLKHMVDD KIHSRARGPI QILNRQPMEG RSRDGGLRFG EMERDCQIAH GAAQFLRERL FEASDPYQVH VCN LCGIMA IANTRTHTYE CRGCRNKTQI SLVRMPYACK LLFQELMSMS IAPRMMSV

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Macromolecule #3: DNA-directed RNA polymerase II subunit RPB3

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: domestic pig (domestic pig) / Tissue: Thymus
Molecular weightTheoretical: 31.439074 KDa
SequenceString: MPYANQPTVR ITELTDENVK FIIENTDLAV ANSIRRVFIA EVPIIAIDWV QIDANSSVLH DEFIAHRLGL IPLTSDDIVD KLQYSRDCT CEEFCPECSV EFTLDVRCNE DQTRHVTSRD LISNSPRVIP VTSRNRDNDP SDYVEQDDIL IVKLRKGQEL R LRAYAKKG ...String:
MPYANQPTVR ITELTDENVK FIIENTDLAV ANSIRRVFIA EVPIIAIDWV QIDANSSVLH DEFIAHRLGL IPLTSDDIVD KLQYSRDCT CEEFCPECSV EFTLDVRCNE DQTRHVTSRD LISNSPRVIP VTSRNRDNDP SDYVEQDDIL IVKLRKGQEL R LRAYAKKG FGKEHAKWNP TAGVAFEYDP DNALRHTVYP KPEEWPKSEY SELDEDESQA PYDPNGKPER FYYNVESCGS LR PETIVLS ALSGLKKKLS DLQTQLSHEI QSDVLTIN

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Macromolecule #4: RNA polymerase II subunit D

MacromoleculeName: RNA polymerase II subunit D / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: domestic pig (domestic pig) / Tissue: Thymus
Molecular weightTheoretical: 16.331255 KDa
SequenceString:
MAAGGSDPRA GDVEEDASQL IFPKEFETAE TLLNSEVHML LEHRKQQNES AEDEQELSEV FMKTLNYTAR FSRFKNRETI ASVRSLLLQ KKLHKFELAC LANLCPETAE ESKALIPSLE GRFEDEELQQ ILDDIQTKRS FQY

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Macromolecule #5: DNA-directed RNA polymerase II subunit E

MacromoleculeName: DNA-directed RNA polymerase II subunit E / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: domestic pig (domestic pig)
Molecular weightTheoretical: 24.644318 KDa
SequenceString: MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQFGDKPSE GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKV YCQRMQEENI TRALIVVQQG MTPSAKQSLV DMAPKYILEQ FLQQELLINI TEHELVPEHV VMTKEEVTEL L ARYKLREN ...String:
MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQFGDKPSE GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKV YCQRMQEENI TRALIVVQQG MTPSAKQSLV DMAPKYILEQ FLQQELLINI TEHELVPEHV VMTKEEVTEL L ARYKLREN QLPRIQAGDP VARYFGIKRG QVVKIIRPSE TAGRYITYRL VQ

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Macromolecule #6: DNA-directed RNA polymerase II subunit F

MacromoleculeName: DNA-directed RNA polymerase II subunit F / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: domestic pig (domestic pig) / Tissue: thymus
Molecular weightTheoretical: 14.477001 KDa
SequenceString:
MSDNEDNFDG DDFDDVEEDE GLDDLENAEE EGQENVEILP SGERPQANQK RITTPYMTKY ERARVLGTRA LQIAMCAPVM VELEGETDP LLIAMKELKA RKIPIIIRRY LPDGSYEDWG VDELIISD

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Macromolecule #7: DNA-directed RNA polymerase II subunit RPB7

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB7 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: domestic pig (domestic pig)
Molecular weightTheoretical: 19.314283 KDa
SequenceString:
MFYHISLEHE ILLHPRYFGP NLLNTVKQKL FTEVEGTCTG KYGFVIAVTT IDNIGAGVIQ PGRGFVLYPV KYKAIVFRPF KGEVVDAVV TQVNKVGLFT EIGPMSCFIS RHSIPSEMEF DPNSNPPCYK TMDEDIVIQQ DDEIRLKIVG TRVDKNDIFA I GSLMDDYL GLVS

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Macromolecule #8: DNA-directed RNA polymerases I, II, and III subunit RPABC3

MacromoleculeName: DNA-directed RNA polymerases I, II, and III subunit RPABC3
type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: domestic pig (domestic pig) / Tissue: Thymus
Molecular weightTheoretical: 17.162273 KDa
SequenceString:
MAGILFEDIF DVKDIDPEGK KFDRVSRLHC ESESFKMDLI LDVNIQIYPV DLGDKFRLVI ASTLYEDGTL DDGEYNPTDD RPSRADQFE YVMYGKVYRI EGDETSTEAA TRLSAYVSYG GLLMRLQGDA NNLHGFEVDS RVYLLMKKLA F

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Macromolecule #9: DNA-directed RNA polymerase II subunit RPB9

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB9 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: domestic pig (domestic pig) / Tissue: Thymus
Molecular weightTheoretical: 14.541221 KDa
SequenceString:
MEPDGTYEPG FVGIRFCQEC NNMLYPKEDK ENRILLYACR NCDYQQEADN SCIYVNKITH EVDELTQIIA DVSQDPTLPR TEDHPCQKC GHKEAVFFQS HSARAEDAMR LYYVCTAPHC GHRWTE

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Macromolecule #10: DNA-directed RNA polymerases I, II, and III subunit RPABC5

MacromoleculeName: DNA-directed RNA polymerases I, II, and III subunit RPABC5
type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: domestic pig (domestic pig) / Tissue: Thymus
Molecular weightTheoretical: 7.655123 KDa
SequenceString:
MIIPVRCFTC GKIVGNKWEA YLGLLQAEYT EGDALDALGL KRYCCRRMLL AHVDLIEKLL NYAPLEK

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Macromolecule #11: RNA_pol_L_2 domain-containing protein

MacromoleculeName: RNA_pol_L_2 domain-containing protein / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: domestic pig (domestic pig) / Tissue: thymus
Molecular weightTheoretical: 13.310284 KDa
SequenceString:
MNAPPAFESF LLFEGEKKIT INKDTKVPNA CLFTINKEDH TLGNIIKSQL LKDPQVLFAG YKVPHPLEHK IIIRVQTTPD YSPQEAFTN AITDLISELS LLEERFRVAI KDKQEGIE

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Macromolecule #12: RNA polymerase II subunit K

MacromoleculeName: RNA polymerase II subunit K / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: domestic pig (domestic pig) / Tissue: thymus
Molecular weightTheoretical: 7.018244 KDa
SequenceString:
MDTQKDVQPP KQQPMIYICG ECHTENEIKS RDPIRCRECG YRIMYKKRTK RLVVFDAR

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Macromolecule #13: RPBI C-terminal domain peptide

MacromoleculeName: RPBI C-terminal domain peptide / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: domestic pig (domestic pig) / Tissue: thymus
Molecular weightTheoretical: 1.356393 KDa
SequenceString:
SPSYSPTSPS YSP

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Macromolecule #17: Negative elongation factor A

MacromoleculeName: Negative elongation factor A / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 57.343598 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MASMRESDTG LWLHNKLGAT DELWAPPSIA SLLTAAVIDN IRLCFHGLSS AVKLKLLLGT LHLPRRTVDE MKGALMEIIQ LASLDSDPW VLMVADILKS FPDTGSLNLE LEEQNPNVQD ILGELREKVG ECEASAMLPL ECQYLNKNAL TTLAGPLTPP V KHFQLKRK ...String:
MASMRESDTG LWLHNKLGAT DELWAPPSIA SLLTAAVIDN IRLCFHGLSS AVKLKLLLGT LHLPRRTVDE MKGALMEIIQ LASLDSDPW VLMVADILKS FPDTGSLNLE LEEQNPNVQD ILGELREKVG ECEASAMLPL ECQYLNKNAL TTLAGPLTPP V KHFQLKRK PKSATLRAEL LQKSTETAQQ LKRSAGVPFH AKGRGLLRKM DTTTPLKGIP KQAPFRSPTA PSVFSPTGNR TP IPPSRTL LRKERGVKLL DISELDMVGA GREAKRRRKT LDAEVVEKPA KEETVVENAT PDYAAGLVST QKLGSLNNEP ALP STSYLP STPSVVPASS YIPSSETPPA PSSREASRPP EEPSAPSPTL PAQFKQRAPM YNSGLSPATP TPAAPTSPLT PTTP PAVAP TTQTPPVAMV APQTQAPAQQ QPKKNLSLTR EQMFAAQEMF KTANKVTRPE KALILGFMAG SRENPCQEQG DVIQI KLSE HTEDLPKADG QGSTTMLVDT VFEMNYATGQ WTRFKKYKPM TNVS

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Macromolecule #18: Negative elongation factor B

MacromoleculeName: Negative elongation factor B / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 68.672789 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)MFAG LQDLGVANGE ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)MFAG LQDLGVANGE DLKETLTNCT EPLKAIEQFQ TENGVLLPSL QSALPFLDLH GTPRLEFHQS VFDELR DKL LERVSAIASE GKAEERYKKL EDLLEKSFSL VKMPSLQPVV MCVMKHLPKV PEKKLKLVMA DKELYRACAV EVKRQIW QD NQALFGDEVS PLLKQYILEK ESALFSTELS VLHNFFSPSP KTRRQGEVVQ RLTRMVGKNV KLYDMVLQFL RTLFLRTR N VHYCTLRAEL LMSLHDLDVG EICTVDPCHK FTWCLDACIR ERFVDSKRAR ELQGFLDGVK KGQEQVLGDL SMILCDPFA INTLALSTVR HLQELVGQET LPRDSPDLLL LLRLLALGQG AWDMIDSQVF KEPKMEVELI TRFLPMLMSF LVDDYTFNVD QKLPAEEKA PVSYPNTLPE SFTKFLQEQR MACEVGLYYV LHITKQRNKN ALLRLLPGLV ETFGDLAFGD IFLHLLTGNL A LLADEFAL EDFCSSLFDG FFLTASPRKE NVHRHALRLL IHLHPRVAPS KLEALQKALE PTGQSGEAVK ELYSQLGEKL EQ LDHRKPS PAQAAETPAL ELPLPSVPAP APL

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Macromolecule #19: Negative elongation factor C/D

MacromoleculeName: Negative elongation factor C/D / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 68.544078 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)TMAG AVPGAIMDED YYGSAAEWGD EADGGQQEDD S GEGEDDAE VQQECLHKFS ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)TMAG AVPGAIMDED YYGSAAEWGD EADGGQQEDD S GEGEDDAE VQQECLHKFS TRDYIMEPSI FNTLKRYFQA GGSPENVIQL LSENYTAVAQ TVNLLAEWLI QTGVEPVQVQ ET VENHLKS LLIKHFDPRK ADSIFTEEGE TPAWLEQMIA HTTWRDLFYK LAEAHPDCLM LNFTVKLISD AGYQGEITSV STA CQQLEV FSRVLRTSLA TILDGGEENL EKNLPEFAKM VCHGEHTYLF AQAMMSVLAQ EEQGGSAVRR IAQEVQRFAQ EKGH DASQI TLALGTAASY PRACQALGAM LSKGALNPAD ITVLFKMFTS MDPPPVELIR VPAFLDLFMQ SLFKPGARIN QDHKH KYIH ILAYAASVVE TWKKNKRVSI NKDELKSTSK AVETVHNLCC NENKGASELV AELSTLYQCI RFPVVAMGVL KWVDWT VSE PRYFQLQTDH TPVHLALLDE ISTCHQLLHP QVLQLLVKLF ETEHSQLDVM EQLELKKTLL DRMVHLLSRG YVLPVVS YI RKCLEKLDTD ISLIRYFVTE VLDVIAPPYT SDFVQLFLPI LENDSIAGTI KTEGEHDPVT EFIAHCKSNF IMVN

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Macromolecule #20: Negative elongation factor E

MacromoleculeName: Negative elongation factor E / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 1.890321 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

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Macromolecule #21: Transcription elongation factor SPT5

MacromoleculeName: Transcription elongation factor SPT5 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 121.145477 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSDSEDSNFS EEEDSERSSD GEEAEVDEER RSAAGSEKEE EPEDEEEEEE EEEYDEEEEE EDDDRPPKKP RHGGFILDEA DVDDEYEDE DQWEDGAEDI LEKEEIEASN IDNVVLDEDR SGARRLQNLW RDQREEELGE YYMKKYAKSS VGETVYGGSD E LSDDITQQ ...String:
MSDSEDSNFS EEEDSERSSD GEEAEVDEER RSAAGSEKEE EPEDEEEEEE EEEYDEEEEE EDDDRPPKKP RHGGFILDEA DVDDEYEDE DQWEDGAEDI LEKEEIEASN IDNVVLDEDR SGARRLQNLW RDQREEELGE YYMKKYAKSS VGETVYGGSD E LSDDITQQ QLLPGVKDPN LWTVKCKIGE ERATAISLMR KFIAYQFTDT PLQIKSVVAP EHVKGYIYVE AYKQTHVKQA IE GVGNLRL GYWNQQMVPI KEMTDVLKVV KEVANLKPKS WVRLKRGIYK DDIAQVDYVE PSQNTISLKM IPRIDYDRIK ARM SLKDWF AKRKKFKRPP QRLFDAEKIR SLGGDVASDG DFLIFEGNRY SRKGFLFKSF AMSAVITEGV KPTLSELEKF EDQP EGIDL EVVTESTGKE REHNFQPGDN VEVCEGELIN LQGKILSVDG NKITIMPKHE DLKDMLEFPA QELRKYFKMG DHVKV IAGR FEGDTGLIVR VEENFVILFS DLTMHELKVL PRDLQLCSET ASGVDVGGQH EWGELVQLDP QTVGVIVRLE RETFQV LNM YGKVVTVRHQ AVTRKKDNRF AVALDSEQNN IHVKDIVKVI DGPHSGREGE IRHLFRSFAF LHCKKLVENG GMFVCKT RH LVLAGGSKPR DVTNFTVGGF APMSPRISSP MHPSAGGQRG GFGSPGGGSG GMSRGRGRRD NELIGQTVRI SQGPYKGY I GVVKDATEST ARVELHSTCQ TISVDRQRLT TVGSRRPGGM TSTYGRTPMY GSQTPMYGSG SRTPMYGSQT PLQDGSRTP HYGSQTPLHD GSRTPAQSGA WDPNNPNTPS RAEEEYEYAF DDEPTPSPQA YGGTPNPQTP GYPDPSSPQV NPQYNPQTPG TPAMYNTDQ FSPYAAPSPQ GSYQPSPSPQ SYHQVAPSPA GYQNTHSPAS YHPTPSPMAY QASPSPSPVG YSPMTPGAPS P GGYNPHTP GSGIEQNSSD WVTTDIQVKV RDTYLDTQVV GQTGVIRSVT GGMCSVYLKD SEKVVSISSE HLEPITPTKN NK VKVILGE DREATGVLLS IDGEDGIVRM DLDEQLKILN LRFLGKLLEA

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Macromolecule #22: Integrator complex subunit 1

MacromoleculeName: Integrator complex subunit 1 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 244.574922 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MNRAKPTTVR RPSAAAKPSG HPPPGDFIAL GSKGQANESK TASTLLKPAP SGLPSERKRD AAAALSSASA LTGLTKRPKL SSTPPLSAL GRLAEAAVAE KRAISPSIKE PSVVPIEVLP TVLLDEIEAA ELEGNDDRIE GVLCGAVKQL KVTRAKPDST L YLSLMYLA ...String:
MNRAKPTTVR RPSAAAKPSG HPPPGDFIAL GSKGQANESK TASTLLKPAP SGLPSERKRD AAAALSSASA LTGLTKRPKL SSTPPLSAL GRLAEAAVAE KRAISPSIKE PSVVPIEVLP TVLLDEIEAA ELEGNDDRIE GVLCGAVKQL KVTRAKPDST L YLSLMYLA KIKPNIFATE GVIEALCSLL RRDASINFKA KGNSLVSVLA CNLLMAAYEE DENWPEIFVK VYIEDSLGER IW VDSPHCK TFVDNIQTAF NTRMPPRSVL LQGEAGRVAG DLGAGSSPHP SLTEEEDSQT ELLIAEEKLS PEQEGQLMPR YEE LAESVE EYVLDMLRDQ LNRRQPIDNV SRNLLRLLTS TCGYKEVRLL AVQKLEMWLQ NPKLTRPAQD LLMSVCMNCN THGS EDMDV ISHLIKIRLK PKVLLNHFML CIRELLSAHK DNLGTTIKLV IFNELSSARN PNNMQVLYTA LQHSSELAPK FLAMV FQDL LTNKDDYLRA SRALLREIIK QTKHEINFQA FCLGLMQERK EPQYLEMEFK ERFVVHITDV LAVSMMLGIT AQVKEA GIA WDKGEKRNLE VLRSFQNQIA AIQRDAVWWL HTVVPSISKL APKDYVHCLH KVLFTEQPET YYKWDNWPPE SDRNFFL RL CSEVPILEDT LMRILVIGLS RELPLGPADA MELADHLVKR AAAVQADDVE VLKVGRTQLI DAVLNLCTYH HPENIQLP P GYQPPNLAIS TLYWKAWPLL LVVAAFNPEN IGLAAWEEYP TLKMLMEMVM TNNYSYPPCT LTDEETRTEM LNRELQTAQ REKQEILAFE GHLAAASTKQ TITESSSLLL SQLTSLDPQG PPRRPPPHIL DQVKSLNQSL RLGHLLCRSR NPDFLLHIIQ RQASSQSMP WLADLVQSSE GSLDVLPVQC LCEFLLHDAV DDAASGEEDD EGESKEQKAK KRQRQQKQRQ LLGRLQDLLL G PKADEQTT CEVLDYFLRR LGSSQVASRV LAMKGLSLVL SEGSLRDGEE KEPPMEEDVG DTDVLQGYQW LLRDLPRLPL FD SVRSTTA LALQQAIHME TDPQTISAYL IYLSQHTPVE EQAQHSDLAL DVARLVVERS TIMSHLFSKL SPSAASDAVL SAL LSIFSR YVRRMRQSKE GEEVYSWSES QDQVFLRWSS GETATMHILV VHAMVILLTL GPPRADDSEF QALLDIWFPE EKPL PTAFL VDTSEEALLL PDWLKLRMIR SEVLRLVDAA LQDLEPQQLL LFVQSFGIPV SSMSKLLQFL DQAVAHDPQT LEQNI MDKN YMAHLVEVQH ERGASGGQTF HSLLTASLPP RRDSTEAPKP KSSPEQPIGQ GRIRVGTQLR VLGPEDDLAG MFLQIF PLS PDPRWQSSSP RPVALALQQA LGQELARVVQ GSPEVPGITV RVLQALATLL SSPHGGALVM SMHRSHFLAC PLLRQLC QY QRCVPQDTGF SSLFLKVLLQ MLQWLDSPGV EGGPLRAQLR MLASQASAGR RLSDVRGGLL RLAEALAFRQ DLEVVSST V RAVIATLRSG EQCSVEPDLI SKVLQGLIEV RSPHLEELLT AFFSATADAA SPFPACKPVV VVSSLLLQEE EPLAGGKPG ADGGSLEAVR LGPSSGLLVD WLEMLDPEVV SSCPDLQLRL LFSRRKGKGQ AQVPSFRPYL LTLFTHQSSW PTLHQCIRVL LGKSREQRF DPSASLDFLW ACIHVPRIWQ GRDQRTPQKR REELVLRVQG PELISLVELI LAEAETRSQD GDTAACSLIQ A RLPLLLSC CCGDDESVRK VTEHLSGCIQ QWGDSVLGRR CRDLLLQLYL QRPELRVPVP EVLLHSEGAA SSSVCKLDGL IH RFITLLA DTSDSRALEN RGADASMACR KLAVAHPLLL LRHLPMIAAL LHGRTHLNFQ EFRQQNHLSC FLHVLGLLEL LQP HVFRSE HQGALWDCLL SFIRLLLNYR KSSRHLAAFI NKFVQFIHKY ITYNAPAAIS FLQKHADPLH DLSFDNSDLV MLKS LLAGL SLPSRDDRTD RGLDEEGEEE SSAGSLPLVS VSLFTPLTAA EMAPYMKRLS RGQTVEDLLE VLSDIDEMSR RRPEI LSFF STNLQRLMSS AEECCRNLAF SLALRSMQNS PSIAAAFLPT FMYCLGSQDF EVVQTALRNL PEYALLCQEH AAVLLH RAF LVGMYGQMDP SAQISEALRI LHMEAVM

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Macromolecule #23: Integrator complex subunit 2

MacromoleculeName: Integrator complex subunit 2 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 134.451625 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MKDQQTVIMT ECTSLQFVSP FAFEAMQKVD VVCLASLSDP ELRLLLPCLV RMALCAPADQ SQSWAQDKKL ILRLLSGVEA VNSIVALLS VDFHALEQDA SKEQQLRHKL GGGSGESILV SQLQHGLTLE FEHSDSPRRL RLVLSELLAI MNKVSESNGE F FFKSSELF ...String:
MKDQQTVIMT ECTSLQFVSP FAFEAMQKVD VVCLASLSDP ELRLLLPCLV RMALCAPADQ SQSWAQDKKL ILRLLSGVEA VNSIVALLS VDFHALEQDA SKEQQLRHKL GGGSGESILV SQLQHGLTLE FEHSDSPRRL RLVLSELLAI MNKVSESNGE F FFKSSELF ESPVYLEEAA DVLCILQAEL PSLLPIVDVA EALLHVRNGA WFLCLLVANV PDSFNEVCRG LIKNGERQDE ES LGGRRRT DALRFLCKMN PSQALKVRGM VVEECHLPGL GVALTLDHTK NEACEDGVSD LVCFVSGLLL GTNAKVRTWF GTF IRNGQQ RKRETSSSVL WQMRRQLLLE LMGILPTVRS TRIVEEADVD MEPNVSVYSG LKEEHVVKAS ALLRLYCALM GIAG LKPTE EEAEQLLQLM TSRPPATPAG VRFVSLSFCM LLAFSTLVST PEQEQLMVVW LSWMIKEEAY FESTSGVSAS FGEML LLVA MYFHSNQLSA IIDLVCSTLG MKIVIKPSSL SRMKTIFTQE IFTEQVVTAH AVRVPVTSNL SANITGFLPI HCIYQL LRS RSFTKHKVSI KDWIYRQLCE TSTPLHPQLL PLIDVYINSI LTPASKSNPE ATNQPVTEQE ILNIFQGVIG GDNIRLN QR FSITAQLLVL YYILSYEEAL LANTKTLAAM QRKPKSYSSS LMDQIPIKFL IRQAQGLQQE LGGLHSALLR LLATNYPH L CIVDDWICEE EITGTDALLR RMLLTNNAKN HSPKQLQEAF SAVPVNNTQV MQIIEHLTLL SASELIPYAE VLTSNMSQL LNSGVPRRIL QTVNKLWMVL NTVMPRRLWV MTVNALQPSI KFVRQQKYTQ NDLMIDPLIV LRCDQRVHRC PPLMDITLHM LNGYLLASK AYLSAHLKET EQDRPSQNNT IGLVGQTDAP EVTREELKNA LLAAQDSAAV QILLEICLPT EEEKANGVNP D SLLRNVQS VITTSAPNKG MEEGEDNLLC NLREVQCLIC CLLHQMYIAD PNIAKLVHFQ GYPCELLPLT VAGIPSMHIC LD FIPELIA QPELEKQIFA IQLLSHLCIQ YALPKSLSVA RLAVNVMGTL LTVLTQAKRY AFFMPTLPSL VSFCRAFPPL YED IMSLLI QIGQVCASDV ATQTRDIDPI ITRLQQIKEK PSGWSQICKD SSYKNGSRDT GSMDPDVQLC HCIERTVIEI INMS VSGI

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Macromolecule #24: Integrator complex subunit 4

MacromoleculeName: Integrator complex subunit 4 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 108.306758 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MAAHLKKRVY EEFTKVVQPQ EEIATKKLRL TKPSKSAALH IDLCKATSPA DALQYLLQFA RKPVEAESVE GVVRILLEHY YKENDPSVR LKIASLLGLL SKTAGFSPDC IMDDAINILQ NEKSHQVLAQ LLDTLLAIGT KLPENQAIQM RLVDVACKHL T DTSHGVRN ...String:
MAAHLKKRVY EEFTKVVQPQ EEIATKKLRL TKPSKSAALH IDLCKATSPA DALQYLLQFA RKPVEAESVE GVVRILLEHY YKENDPSVR LKIASLLGLL SKTAGFSPDC IMDDAINILQ NEKSHQVLAQ LLDTLLAIGT KLPENQAIQM RLVDVACKHL T DTSHGVRN KCLQLLGNLG SLEKSVTKDA EGLAARDVQK IIGDYFSDQD PRVRTAAIKA MLQLHERGLK LHQTIYNQAC KL LSDDYEQ VRSAAVQLIW VVSQLYPESI VPIPSSNEEI RLVDDAFGKI CHMVSDGSWV VRVQAAKLLG SMEQVSSHFL EQT LDKKLM SDLRRKRTAH ERAKELYSSG EFSSGRKWGD DAPKEEVDTG AVNLIESGAC GAFVHGLEDE MYEVRIAAVE ALCM LAQSS PSFAEKCLDF LVDMFNDEIE EVRLQSIHTM RKISNNITLR EDQLDTVLAV LEDSSRDIRE ALHELLCCTN VSTKE GIHL ALVELLKNLT KYPTDRDSIW KCLKFLGSRH PTLVLPLVPE LLSTHPFFDT AEPDMDDPAY IAVLVLIFNA AKTCPT MPA LFSDHTFRHY AYLRDSLSHL VPALRLPGRK LVSSAVSPSI IPQEDPSQQF LQQSLERVYS LQHLDPQGAQ ELLEFTI RD LQRLGELQSE LAGVADFSAT YLRCQLLLIK ALQEKLWNVA APLYLKQSDL ASAAAKQIME ETYKMEFMYS GVENKQVV I IHHMRLQAKA LQLIVTARTT RGLDPLFGMC EKFLQEVDFF QRYFIADLPH LQDSFVDKLL DLMPRLMTSK PAEVVKILQ TMLRQSAFLH LPLPEQIHKA SATIIEPAGE SDNPLRFTSG LVVALDVDAT LEHVQDPQNT VKVQVLYPDG QAQMIHPKPA DFRNPGPGR HRLITQVYLS HTAWTEACQV EVRLLLAYNS SARIPKCPWM EGGEMSPQVE TSIEGTIPFS KPVKVYIMPK P ARR

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Macromolecule #25: Integrator complex subunit 5

MacromoleculeName: Integrator complex subunit 5 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 108.115227 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSALCDPPGA PGPPGPAPAT HGPAPLSAQE LSQEIKAFLT GVDPILGHQL SAREHARCGL LLLRSLPPAR AAVLDHLRGV FDESVRAHL AALDETPVAG PPHLRPPPPS HVPAGGPGLE DVVQEVQQVL SEFIRANPKA WAPVISAWSI DLMGQLSSTY S GQHQRVPH ...String:
MSALCDPPGA PGPPGPAPAT HGPAPLSAQE LSQEIKAFLT GVDPILGHQL SAREHARCGL LLLRSLPPAR AAVLDHLRGV FDESVRAHL AALDETPVAG PPHLRPPPPS HVPAGGPGLE DVVQEVQQVL SEFIRANPKA WAPVISAWSI DLMGQLSSTY S GQHQRVPH ATGALNELLQ LWMGCRATRT LMDIYVQCLS ALIGSCPDAC VDALLDTSVQ HSPHFDWVVA HIGSSFPGTI IS RVLSCGL KDFCVHGGAG GGAGSSGGSS SQTPSTDPFP GSPAIPAEKR VPKIASVVGI LGHLASRHGD SIRRELLRMF HDS LAGGSG GRSGDPSLQA TVPFLLQLAV MSPALLGTVS GELVDCLKPP AVLSQLQQHL QGFPREELDN MLNLAVHLVS QASG AGAYR LLQFLVDTAM PASVITTQGL AVPDTVREAC DRLIQLLLLH LQKLVHHRGG SPGEGVLGPP PPPRLVPFLD ALKNH VGEL CGETLRLERK RFLWQHQLLG LLSVYTRPSC GPEALGHLLS RARSPEELSL ATQLYAGLVV SLSGLLPLAF RSCLAR VHA GTLQPPFTAR FLRNLALLVG WEQQGGEGPA ALGAHFGESA SAHLSDLAPL LLHPEEEVAE AAASLLAICP FPSEALS PS QLLGLVRAGV HRFFASLRLH GPPGVASACQ LLTRLSQTSP AGLKAVLQLL VEGALHRGNT ELFGGQVDGD NETLSVVS A SLASASLLDT NRRHTAAVPG PGGIWSVFHA GVIGRGLKPP KFVQSRNQQE VIYNTQSLLS LLVHCCSAPG GTECGECWG APILSPEAAK AVAVTLVESV CPDAAGAELA WPPEEHARAT VERDLRIGRR FREQPLLFEL LKLVAAAPPA LCYCSVLLRG LLAALLGHW EASRHPDTTH SPWHLEASCT LVAVMAEGSL LPPALGNMHE VFSQLAPFEV RLLLLSVWGF LREHGPLPQK F IFQSERGR FIRDFSREGG GEGGPHLAVL HSVLHRNIDR LGLFSGRFQA PSPSTLLRQG T

+
Macromolecule #26: Integrator complex subunit 6

MacromoleculeName: Integrator complex subunit 6 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 100.527078 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MPILLFLIDT SASMNQRSHL GTTYLDTAKG AVETFMKLRA RDPASRGDRY MLVTFEEPPY AIKAGWKENH ATFMNELKNL QAEGLTTLG QSLRTAFDLL NLNRLVTGID NYGQGRNPFF LEPAIIITIT DGSKLTTTSG VQDELHLPLN SPLPGSELTK E PFRWDQRL ...String:
MPILLFLIDT SASMNQRSHL GTTYLDTAKG AVETFMKLRA RDPASRGDRY MLVTFEEPPY AIKAGWKENH ATFMNELKNL QAEGLTTLG QSLRTAFDLL NLNRLVTGID NYGQGRNPFF LEPAIIITIT DGSKLTTTSG VQDELHLPLN SPLPGSELTK E PFRWDQRL FALVLRLPGT MSVESEQLTG VPLDDSAITP MCEVTGGRSY SVCSPRMLNQ CLESLVQKVQ SGVVINFEKA GP DPSPVED GQPDISRPFG SQPWHSCHKL IYVRPNPKTG VPIGHWPVPE SFWPDQNSPT LPPRTSHPVV KFSCTDCEPM VID KLPFDK YELEPSPLTQ FILERKSPQT CWQVYVSNSA KYSELGHPFG YLKASTALNC VNLFVMPYNY PVLLPLLDDL FKVH KAKPT LKWRQSFESY LKTMPPYYLG PLKKAVRMMG APNLIADSME YGLSYSVISY LKKLSQQAKI ESDRVIGSVG KKVVQ ETGI KVRSRSHGLS MAYRKDFQQL LQGISEDVPH RLLDLNMKEY TGFQVALLNK DLKPQTFRNA YDIPRRNLLD HLTRMR SNL LKSTRRFLKG QDEDQVHSVP IAQMGNYQEY LKQVPSPLRE LDPDQPRRLH TFGNPFKLDK KGMMIDEADE FVAGPQN KH KRPGEPNMQG IPKRRRCMSP LLRGRQQNPV VNNHIGGKGP PAPTTQAQPD LIKPLPLHKI SETTNDSIIH DVVENHVA D QLSSDITPNA MDTEFSASSP ASLLERPTNH MEALGHDHLG TNDLTVGGFL ENHEEPRDKE QCAEENIPAS SLNKGKKLM HCRSHEEVNT ELKAQIMKEI RKPGRKYERI FTLLKHVQGS LQTRLIFLQN VIKEASRFKK RMLIEQLENF LDEIHRRANQ INHINSN

+
Macromolecule #27: Integrator complex subunit 7

MacromoleculeName: Integrator complex subunit 7 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 106.952617 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MASNSTKSFL ADAGYGEQEL DANSALMELD KGLRSGKLGE QCEAVVRFPR LFQKYPFPIL INSAFLKLAD VFRVGNNFLR LCVLKVTQQ SEKHLEKILN VDEFVKRIFS VIHSNDPVAR AITLRMLGSL ASIIPERKNA HHSIRQSLDS HDNVEVEAAV F AAANFSAQ ...String:
MASNSTKSFL ADAGYGEQEL DANSALMELD KGLRSGKLGE QCEAVVRFPR LFQKYPFPIL INSAFLKLAD VFRVGNNFLR LCVLKVTQQ SEKHLEKILN VDEFVKRIFS VIHSNDPVAR AITLRMLGSL ASIIPERKNA HHSIRQSLDS HDNVEVEAAV F AAANFSAQ SKDFAVGICN KISEMIQGLA TPVDLKLKLI PILQHMHHDA ILASSARQLL QQLVTSYPST KMVIVSLHTF TL LAASSLV DTPKQIQLLL QYLKNDPRKA VKRLAIQDLK LLANKTPHTW SRENIQALCE CALQTPYDSL KLGMLSVLST LSG TIAIKH YFSIVPGNVS SSPRSSDLVK LAQECCYHNN RGIAAHGVRV LTNITVSCQE KDLLALEQDA VFGLESLLVL CSQD DSPGA QATLKIALNC MVKLAKGRPH LSQSVVETLL TQLHSAQDAA RILMCHCLAA IAMQLPVLGD GMLGDLMELY KVIGR SATD KQQELLVSLA TVIFVASQKA LSVESKAVIK QQLESVSNGW TVYRIARQAS RMGNHDMAKE LYQSLLTQVA SEHFYF WLN SLKEFSHAEQ CLTGLQEENY SSALSCIAES LKFYHKGIAS LTAASTPLNP LSFQCEFVKL RIDLLQAFSQ LICTCNS LK TSPPPAIATT IAMTLGNDLQ RCGRISNQMK QSMEEFRSLA SRYGDLYQAS FDADSATLRN VELQQQSCLL ISHAIEAL I LDPESASFQE YGSTGTAHAD SEYERRMMSV YNHVLEEVES LNRKYTPVSY MHTACLCNAI IALLKVPLSF QRYFFQKLQ STSIKLALSP SPRNPAEPIA VQNNQQLALK VEGVVQHGSK PGLFRKIQSV CLNVSSTLQS KSGQDYKIPI DNMTNEMEQR VEPHNDYFS TQFLLNFAIL GTHNITVESS VKDANGIVWK TGPRTTIFVK SLEDPYSQQI RLQQQQAQQP LQQQQQRNAY T RF

+
Macromolecule #28: Integrator complex subunit 8

MacromoleculeName: Integrator complex subunit 8 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 113.219859 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSAEAADREA ATSSRPCTPP QTCWFEFLLE ESLLEKHLRK PCPDPAPVQL IVQFLEQASK PSVNEQNQVQ PPPDNKRNRI LKLLALKVA AHLKWDLDIL EKSLSVPVLN MLLNELLCIS KVPPGTKHVD MDLATLPPTT AMAVLLYNRW AIRTIVQSSF P VKQAKPGP ...String:
MSAEAADREA ATSSRPCTPP QTCWFEFLLE ESLLEKHLRK PCPDPAPVQL IVQFLEQASK PSVNEQNQVQ PPPDNKRNRI LKLLALKVA AHLKWDLDIL EKSLSVPVLN MLLNELLCIS KVPPGTKHVD MDLATLPPTT AMAVLLYNRW AIRTIVQSSF P VKQAKPGP PQLSVMNQMQ QEKELTENIL KVLKEQAADS ILVLEAALKL NKDLYVHTMR TLDLLAMEPG MVNGETESST AG LKVKTEE MQCQVCYDLG AAYFQQGSTN SAVYENAREK FFRTKELIAE IGSLSLHCTI DEKRLAGYCQ ACDVLVPSSD STS QQLTPY SQVHICLRSG NYQEVIQIFI EDNLTLSLPV QFRQSVLREL FKKAQQGNEA LDEICFKVCA CNTVRDILEG RTIS VQFNQ LFLRPNKEKI DFLLEVCSRS VNLEKASESL KGNMAAFLKN VCLGLEDLQY VFMISSHELF ITLLKDEERK LLVDQ MRKR SPRVNLCIKP VTSFYDIPAS ASVNIGQLEH QLILSVDPWR IRQILIELHG MTSERQFWTV SNKWEVPSVY SGVILG IKD NLTRDLVYIL MAKGLHCSTV KDFSHAKQLF AACLELVTEF SPKLRQVMLN EMLLLDIHTH EAGTGQAGER PPSDLIS RV RGYLEMRLPD IPLRQVIAEE CVAFMLNWRE NEYLTLQVPA FLLQSNPYVK LGQLLAATCK ELPGPKESRR TAKDLWEV V VQICSVSSQH KRGNDGRVSL IKQRESTLGI MYRSELLSFI KKLREPLVLT IILSLFVKLH NVREDIVNDI TAEHISIWP SSIPNLQSVD FEAVAITVKE LVRYTLSINP NNHSWLIIQA DIYFATNQYS AALHYYLQAG AVCSDFFNKA VPPDVYTDQV IKRMIKCCS LLNCHTQVAI LCQFLREIDY KTAFKSLQEQ NSHDAMDSYY DYIWDVTILE YLTYLHHKRG ETDKRQIAIK A IGQTELNA SNPEEVLQLA AQRRKKKFLQ AMAKLYF

+
Macromolecule #29: Integrator complex subunit 9

MacromoleculeName: Integrator complex subunit 9 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 73.891219 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MKLYCLSGHP TLPCNVLKFK STTIMLDCGL DMTSTLNFLP LPLVQSPRLS NLPGWSLKDG NAFLDKELKE CSGHVFVDSV PEFCLPETE LIDLSTVDVI LISNYHCMMA LPYITEHTGF TGTVYATEPT VQIGRLLMEE LVNFIERVPK AQSASLWKNK D IQRLLPSP ...String:
MKLYCLSGHP TLPCNVLKFK STTIMLDCGL DMTSTLNFLP LPLVQSPRLS NLPGWSLKDG NAFLDKELKE CSGHVFVDSV PEFCLPETE LIDLSTVDVI LISNYHCMMA LPYITEHTGF TGTVYATEPT VQIGRLLMEE LVNFIERVPK AQSASLWKNK D IQRLLPSP LKDAVEVSTW RRCYTMQEVN SALSKIQLVG YSQKIELFGA VQVTPLSSGY ALGSSNWIIQ SHYEKVSYVS GS SLLTTHP QPMDQASLKN SDVLVLTGLT QIPTANPDGM VGEFCSNLAL TVRNGGNVLV PCYPSGVIYD LLECLYQYID SAG LSSVPL YFISPVANSS LEFSQIFAEW LCHNKQSKVY LPEPPFPHAE LIQTNKLKHY PSIHGDFSND FRQPCVVFTG HPSL RFGDV VHFMELWGKS SLNTVIFTEP DFSYLEALAP YQPLAMKCIY CPIDTRLNFI QVSKLLKEVQ PLHVVCPEQY TQPPP AQSH RMDLMIDCQP PAMSYRRAEV LALPFKRRYE KIEIMPELAD SLVPMEIKPG ISLATVSAVL HTKDNKHLLQ PPPRPA QPT SGKKRKRVSD DVPDCKVLKP LLSGSIPVEQ FVQTLEKHGF SDIKVEDTAK GHIVLLQEAE TLIQIEEDST HIICDND EM LRVRLRDLVL KFLQKF

+
Macromolecule #30: Integrator complex subunit 11

MacromoleculeName: Integrator complex subunit 11 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
EC number: Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 67.770586 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MPEIRVTPLG AGQDVGRSCI LVSIAGKNVM LDCGMHMGFN DDRRFPDFSY ITQNGRLTDF LDCVIISHFH LDHCGALPYF SEMVGYDGP IYMTHPTQAI CPILLEDYRK IAVDKKGEAN FFTSQMIKDC MKKVVAVHLH QTVQVDDELE IKAYYAGHVL G AAMFQIKV ...String:
MPEIRVTPLG AGQDVGRSCI LVSIAGKNVM LDCGMHMGFN DDRRFPDFSY ITQNGRLTDF LDCVIISHFH LDHCGALPYF SEMVGYDGP IYMTHPTQAI CPILLEDYRK IAVDKKGEAN FFTSQMIKDC MKKVVAVHLH QTVQVDDELE IKAYYAGHVL G AAMFQIKV GSESVVYTGD YNMTPDRHLG AAWIDKCRPN LLITESTYAT TIRDSKRCRE RDFLKKVHET VERGGKVLIP VF ALGRAQE LCILLETFWE RMNLKVPIYF STGLTEKANH YYKLFIPWTN QKIRKTFVQR NMFEFKHIKA FDRAFADNPG PMV VFATPG MLHAGQSLQI FRKWAGNEKN MVIMPGYCVQ GTVGHKILSG QRKLEMEGRQ VLEVKMQVEY MSFSAHADAK GIMQ LVGQA EPESVLLVHG EAKKMEFLKQ KIEQELRVNC YMPANGETVT LPTSPSIPVG ISLGLLKREM AQGLLPEAKK PRLLH GTLI MKESNFRLVS SEQALKELGL AEHQLRFTCR VHLHDTRKEQ ETALRVYSHL KSVLKDHCVQ HLPDGSVTVE SVLLQA AAP SEDPGTKVLL VSWTYQDEEL GSFLTSLLKK GLPQAPS

+
Macromolecule #31: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit...

MacromoleculeName: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 65.378344 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MAAADGDDSL YPIAVLIDEL RNEDVQLRLN SIKKLSTIAL ALGVERTRSE LLPFLTDTIY DEDEVLLALA EQLGTFTTLV GGPEYVHCL LPPLESLATV EETVVRDKAV ESLRAISHEH SPSDLEAHFV PLVKRLAGGD WFTSRTSACG LFSVCYPRVS S AVKAELRQ ...String:
MAAADGDDSL YPIAVLIDEL RNEDVQLRLN SIKKLSTIAL ALGVERTRSE LLPFLTDTIY DEDEVLLALA EQLGTFTTLV GGPEYVHCL LPPLESLATV EETVVRDKAV ESLRAISHEH SPSDLEAHFV PLVKRLAGGD WFTSRTSACG LFSVCYPRVS S AVKAELRQ YFRNLCSDDT PMVRRAAASK LGEFAKVLEL DNVKSEIIPM FSNLASDEQD SVRLLAVEAC VNIAQLLPQE DL EALVMPT LRQAAEDKSW RVRYMVADKF TELQKAVGPE ITKTDLVPAF QNLMKDCEAE VRAAASHKVK EFCENLSADC REN VIMSQI LPCIKELVSD ANQHVKSALA SVIMGLSPIL GKDNTIEHLL PLFLAQLKDE CPEVRLNIIS NLDCVNEVIG IRQL SQSLL PAIVELAEDA KWRVRLAIIE YMPLLAGQLG VEFFDEKLNS LCMAWLVDHV YAIREAATSN LKKLVEKFGK EWAHA TIIP KVLAMSGDPN YLHRMTTLFC INVLSEVCGQ DITTKHMLPT VLRMAGDPVA NVRFNVAKSL QKIGPILDNS TLQSEV KPI LEKLTQDQDV DVKYFAQEAL TVLSLA

+
Macromolecule #32: Serine/threonine-protein phosphatase 2A catalytic subunit alpha i...

MacromoleculeName: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO / EC number: protein-serine/threonine phosphatase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 35.635168 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MDEKVFTKEL DQWIEQLNEC KQLSESQVKS LCEKAKEILT KESNVQEVRC PVTVCGDVHG QFHDLMELFR IGGKSPDTNY LFMGDYVNR GYYSVETVTL LVALKVRYRE RITILRGNHE SRQITQVYGF YDECLRKYGN ANVWKYFTDL FDYLPLTALV D GQIFCLHG ...String:
MDEKVFTKEL DQWIEQLNEC KQLSESQVKS LCEKAKEILT KESNVQEVRC PVTVCGDVHG QFHDLMELFR IGGKSPDTNY LFMGDYVNR GYYSVETVTL LVALKVRYRE RITILRGNHE SRQITQVYGF YDECLRKYGN ANVWKYFTDL FDYLPLTALV D GQIFCLHG GLSPSIDTLD HIRALDRLQE VPHEGPMCDL LWSDPDDRGG WGISPRGAGY TFGQDISETF NHANGLTLVS RA HQLVMEG YNWCHDRNVV TIFSAPNYCY RCGNQAAIME LDDTLKYSFL QFDPAPRRGE PHVTRRTPDY FL

+
Macromolecule #33: Unknown

MacromoleculeName: Unknown / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 2.416951 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)W(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

+
Macromolecule #14: Non-template DNA

MacromoleculeName: Non-template DNA / type: dna / ID: 14 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Human immunodeficiency virus 1
Molecular weightTheoretical: 14.712466 KDa
SequenceString:
(DG)(DT)(DA)(DC)(DA)(DC)(DT)(DA)(DG)(DT) (DA)(DC)(DC)(DT)(DA)(DC)(DT)(DC)(DG)(DA) (DG)(DT)(DG)(DA)(DG)(DC)(DT)(DT)(DA) (DA)(DG)(DC)(DC)(DT)(DC)(DA)(DA)(DT)(DA) (DA) (DA)(DG)(DC)(DT)(DT)(DG)(DC)(DC)

+
Macromolecule #16: DNA Template

MacromoleculeName: DNA Template / type: dna / ID: 16 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Human immunodeficiency virus 1
Molecular weightTheoretical: 14.910556 KDa
SequenceString:
(DG)(DG)(DC)(DA)(DA)(DG)(DC)(DT)(DT)(DT) (DA)(DT)(DT)(DG)(DA)(DG)(DG)(DC)(DT)(DT) (DA)(DA)(DG)(DC)(DA)(DG)(DT)(DG)(DG) (DG)(DT)(DT)(DC)(DC)(DA)(DG)(DG)(DT)(DA) (DC) (DT)(DA)(DG)(DT)(DG)(DT)(DA)(DC)

+
Macromolecule #15: TAR RNA

MacromoleculeName: TAR RNA / type: rna / ID: 15 / Number of copies: 1
Source (natural)Organism: Human immunodeficiency virus 1
Molecular weightTheoretical: 5.4103 KDa
SequenceString:
UAACUAGGGA ACCCACU

+
Macromolecule #34: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 34 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #35: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 35 / Number of copies: 10 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #36: MANGANESE (II) ION

MacromoleculeName: MANGANESE (II) ION / type: ligand / ID: 36 / Number of copies: 2 / Formula: MN
Molecular weightTheoretical: 54.938 Da

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.4
GridModel: Quantifoil R3.5/1 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2.7 nm / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 %

-
Electron microscopy

MicroscopeFEI TITAN
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: OTHER
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Number real images: 45000 / Average exposure time: 2.21 sec. / Average electron dose: 46.18 e/Å2

-
Image processing

Particle selectionNumber selected: 4437434
CTF correctionSoftware - Name: Warp
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final 3D classificationSoftware - Name: RELION
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 614283

-
Atomic model buiding 1

Initial model(PDB ID:

6gml

,

7cun

,

7bfp

,

3dw8

)
RefinementSpace: REAL / Protocol: AB INITIO MODEL
Output model

PDB-7pks:
Structural basis of Integrator-mediated transcription regulation

+
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