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- EMDB-12874: CryoEM structure of the outer membrane secretin pore pIV from the... -

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Basic information

Entry
Database: EMDB / ID: EMD-12874
TitleCryoEM structure of the outer membrane secretin pore pIV from the f1 filamentous bacteriophage.
Map dataPostprocessed masked map from Relion
Sample
  • Organelle or cellular component: Virion export protein pIV
    • Protein or peptide: Virion export protein
  • Ligand: 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE
Function / homology
Function and homology information


viral extrusion / protein secretion / host cell membrane / membrane => GO:0016020
Similarity search - Function
GspD/PilQ family / Bacterial type II secretion system protein D signature. / Type II secretion system protein GspD, conserved site / NolW-like superfamily / Type II/III secretion system / Bacterial type II and III secretion system protein
Similarity search - Domain/homology
Virion export protein
Similarity search - Component
Biological speciesInoviridae sp. (virus) / Enterobacteria phage f1 (virus)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.7 Å
AuthorsConners R / Gold VAM
Funding support United Kingdom, 1 items
OrganizationGrant numberCountry
Wellcome Trust210363/Z/18/Z United Kingdom
CitationJournal: Nat Commun / Year: 2021
Title: CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage.
Authors: Rebecca Conners / Mathew McLaren / Urszula Łapińska / Kelly Sanders / M Rhia L Stone / Mark A T Blaskovich / Stefano Pagliara / Bertram Daum / Jasna Rakonjac / Vicki A M Gold /
Abstract: The Ff family of filamentous bacteriophages infect gram-negative bacteria, but do not cause lysis of their host cell. Instead, new virions are extruded via the phage-encoded pIV protein, which has ...The Ff family of filamentous bacteriophages infect gram-negative bacteria, but do not cause lysis of their host cell. Instead, new virions are extruded via the phage-encoded pIV protein, which has homology with bacterial secretins. Here, we determine the structure of pIV from the f1 filamentous bacteriophage at 2.7 Å resolution by cryo-electron microscopy, the first near-atomic structure of a phage secretin. Fifteen f1 pIV subunits assemble to form a gated channel in the bacterial outer membrane, with associated soluble domains projecting into the periplasm. We model channel opening and propose a mechanism for phage egress. By single-cell microfluidics experiments, we demonstrate the potential for secretins such as pIV to be used as adjuvants to increase the uptake and efficacy of antibiotics in bacteria. Finally, we compare the f1 pIV structure to its homologues to reveal similarities and differences between phage and bacterial secretins.
History
DepositionMay 5, 2021-
Header (metadata) releaseNov 3, 2021-
Map releaseNov 3, 2021-
UpdateNov 17, 2021-
Current statusNov 17, 2021Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0226
  • Imaged by UCSF Chimera
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  • Surface view colored by cylindrical radius
  • Surface level: 0.0226
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7ofh
  • Surface level: 0.0226
  • Imaged by UCSF Chimera
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-7ofh
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_12874.png

Downloads & links

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Map

FileDownload / File: emd_12874.map.gz / Format: CCP4 / Size: 42.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationPostprocessed masked map from Relion
Voxel sizeX=Y=Z: 1.072 Å
Density
Contour LevelBy AUTHOR: 0.0226 / Movie #1: 0.0226
Minimum - Maximum-0.10828287 - 0.17869703
Average (Standard dev.)0.00061690575 (±0.007471375)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions224224224
Spacing224224224
CellA=B=C: 240.128 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0721.0721.072
M x/y/z224224224
origin x/y/z0.0000.0000.000
length x/y/z240.128240.128240.128
α/β/γ90.00090.00090.000
start NX/NY/NZ799196
NX/NY/NZ149138117
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS224224224
D min/max/mean-0.1080.1790.001

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Supplemental data

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Additional map: Postprocessed map after DeepEMhancer

Fileemd_12874_additional_1.map
AnnotationPostprocessed map after DeepEMhancer
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Half map1

Fileemd_12874_additional_2.map
AnnotationHalf map1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Half map2

Fileemd_12874_additional_3.map
AnnotationHalf map2
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Virion export protein pIV

EntireName: Virion export protein pIV
Components
  • Organelle or cellular component: Virion export protein pIV
    • Protein or peptide: Virion export protein
  • Ligand: 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE

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Supramolecule #1: Virion export protein pIV

SupramoleculeName: Virion export protein pIV / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1 / Details: From Inoviridae sp. f1
Source (natural)Organism: Inoviridae sp. (virus)
Molecular weightTheoretical: 669.7 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli) / Recombinant strain: TG1 / Recombinant plasmid: pPMR132

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Macromolecule #1: Virion export protein

MacromoleculeName: Virion export protein / type: protein_or_peptide / ID: 1
Details: S9P, D49N, I66N are naturally occurring polymorphisms.
Number of copies: 15 / Enantiomer: LEVO
Source (natural)Organism: Enterobacteria phage f1 (virus)
Molecular weightTheoretical: 44.657707 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: QVIEMNNSPL RDFVTWYSKQ TGESVIVSPD VKGTVTVYSS DVKPENLRNF FISVLRANNF DMVGSNPSII QKYNPNNQDY IDELPSSDN QEYDDNSAPS GGFFVPQNDN VTQTFKINNV RAKDLIRVVE LFVKSNTSKS SNVLSVDGSN LLVVSAPKDI L DNLPQFLS ...String:
QVIEMNNSPL RDFVTWYSKQ TGESVIVSPD VKGTVTVYSS DVKPENLRNF FISVLRANNF DMVGSNPSII QKYNPNNQDY IDELPSSDN QEYDDNSAPS GGFFVPQNDN VTQTFKINNV RAKDLIRVVE LFVKSNTSKS SNVLSVDGSN LLVVSAPKDI L DNLPQFLS TVDLPTDQIL IEGLIFEVQQ GDALDFSFAA GSQRGTVAGG VNTDRLTSVL SSAGGSFGIF NGDVLGLSVR AL KTNSHSK ILSVPRILTL SGQKGSISVG QNVPFITGRV TGESANVNNP FQTVERQNVG ISMSVFPVAM ASAHHHHHHH GGN IVLDIT IKADSLSSST QASDVITNQR SIATTVNLRD GQTLLLGGLT DYKNTSQDSG VPFLSKIPLI GLLFSSRSDS NEES TLYVL VKATIVRAL

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Macromolecule #2: 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE

MacromoleculeName: 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE
type: ligand / ID: 2 / Number of copies: 30 / Formula: CPS
Molecular weightTheoretical: 614.877 Da
Chemical component information

ChemComp-CPS:
3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE / detergent*YM / CHAPS detergent

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.7 mg/mL
BufferpH: 8
Component:
ConcentrationFormulaName
1.0 % (w/v)C32H58N2O7SCHAPS (3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate)
25.0 mMC8H17N2NaO4Ssodium hepes
500.0 mMNaClSodium chloridesodium chloride
0.5 mMC10H16N2O8EDTAEthylenediaminetetraacetic acid
GridModel: PELCO Ultrathin Carbon with Lacey Carbon / Mesh: 300 / Support film - Material: GRAPHENE OXIDE / Support film - topology: LACEY
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV / Details: blot force 0, blot time 4sec.

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Electron microscopy

MicroscopeTFS KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal magnification: 81000
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average exposure time: 3.52 sec. / Average electron dose: 42.059 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: Warp
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION
Final 3D classificationSoftware - Name: RELION
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1beta)
Final reconstructionApplied symmetry - Point group: C15 (15 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 2.7 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1beta) / Number images used: 111679
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

5w68

RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-7ofh:
CryoEM structure of the outer membrane secretin pore pIV from the f1 filamentous bacteriophage.

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