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- EMDB-12567: 39S mammalian mitochondrial large ribosomal subunit with mtRRF (p... -

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Basic information

Entry
Database: EMDB / ID: EMD-12567
Title39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2
Map data
Sample
  • Complex: 39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2
    • Complex: mtRRF (post) and mtEFG2
      • Protein or peptide: x 2 types
    • Complex: 39S mammalian mitochondrial large ribosomal subunit
      • Protein or peptide: x 51 types
      • RNA: x 2 types
  • Ligand: x 6 types
Function / homology
Function and homology information


Mitochondrial translation elongation / Mitochondrial translation termination / ribosome disassembly / mitochondrial translational termination / mitochondrial transcription / mitochondrial translational elongation / translation release factor activity, codon nonspecific / microprocessor complex / Mitochondrial translation termination / ribonuclease III activity ...Mitochondrial translation elongation / Mitochondrial translation termination / ribosome disassembly / mitochondrial translational termination / mitochondrial transcription / mitochondrial translational elongation / translation release factor activity, codon nonspecific / microprocessor complex / Mitochondrial translation termination / ribonuclease III activity / mitochondrial large ribosomal subunit / peptidyl-tRNA hydrolase / aminoacyl-tRNA hydrolase activity / mitochondrial ribosome / mitochondrial translation / ribosomal large subunit binding / organelle membrane / RNA processing / rescue of stalled ribosome / double-stranded RNA binding / large ribosomal subunit / cell junction / negative regulation of translation / rRNA binding / nuclear body / ribosome / mitochondrial matrix / structural constituent of ribosome / cell cycle / ribonucleoprotein complex / translation / protein domain specific binding / nucleotide binding / mRNA binding / intracellular membrane-bounded organelle / GTPase activity / synapse / GTP binding / nucleolus / positive regulation of DNA-templated transcription / mitochondrion / RNA binding / nucleoplasm / nucleus / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Ribosome-releasing factor 2, mitochondrial / Ribosome recycling factor / Ribosome recycling factor domain / RRF superfamily / Ribosome recycling factor / Elongation factor G, domain III / EFG, domain V / Ribosomal protein L37, mitochondrial / Ribosomal protein L55, mitochondrial / Mitochondrial ribosomal protein L48 ...Ribosome-releasing factor 2, mitochondrial / Ribosome recycling factor / Ribosome recycling factor domain / RRF superfamily / Ribosome recycling factor / Elongation factor G, domain III / EFG, domain V / Ribosomal protein L37, mitochondrial / Ribosomal protein L55, mitochondrial / Mitochondrial ribosomal protein L48 / 39S ribosomal protein L40, mitochondrial / Mitochondrial ribosomal protein L55 superfamily / Mitochondrial ribosomal protein L37 / Mitochondrial ribosomal protein L55 / Mitochondrial ribosomal protein L46 NUDIX / Ribosomal protein S30, mitochondrial / Ribosomal protein L53, mitochondrial / 39S ribosomal protein L53/MRP-L53 / 39S ribosomal protein L42, mitochondrial / Mitochondrial 28S ribosomal protein S32 / Ribosomal protein L28/L40, mitochondrial / Mitochondrial ribosomal protein L28 / Ribosomal protein L37/S30 / Ribosomal protein 63, mitochondrial / Growth arrest/ DNA-damage-inducible protein-interacting protein 1 / Ribosomal protein L51, mitochondrial / Mitochondrial 28S ribosomal protein S30 (PDCD9) / Growth arrest and DNA-damage-inducible proteins-interacting protein 1 / Mitochondrial ribosomal subunit / Mitochondrial ribosome protein 63 / Growth arrest and DNA damage-inducible proteins-interacting protein 1 domain superfamily / Ribosomal protein L35, mitochondrial / 39S ribosomal protein L52, mitochondrial / Mitoribosomal protein mL52 / MRPL44, double-stranded RNA binding domain / Tim44-like domain / Ribosomal protein L7/L12, oligomerisation / Ribosomal protein L7/L12, oligomerisation domain superfamily / Tim44-like domain / Ribosomal protein L7/L12 dimerisation domain / Tim44 / Ribosomal protein L7/L12 / Ribosomal protein L7/L12, C-terminal / Ribosomal protein L7/L12 C-terminal domain / Ribosomal protein L49/IMG2 / Mitochondrial large subunit ribosomal protein (Img2) / Ribosomal protein L46, N-terminal / 39S mitochondrial ribosomal protein L46 / Ribosomal protein L50, mitochondria / Ribosomal protein L27/L41, mitochondrial / Mitochondrial ribosomal protein L27 / Ribosomal subunit 39S / 39S ribosomal protein L46, mitochondrial / Ribosomal protein L47, mitochondrial / MRP-L47 superfamily, mitochondrial / 39S ribosomal protein L43/54S ribosomal protein L51 / Mitochondrial 39-S ribosomal protein L47 (MRP-L47) / Phosphatidylethanolamine-binding protein / Phosphatidylethanolamine-binding protein / Phosphatidylethanolamine-binding protein, eukaryotic / Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like / PEBP-like superfamily / Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamily / TGS domain profile. / TGS / TGS-like / Peptide chain release factor class I / RF-1 domain / Ribonuclease III, endonuclease domain superfamily / Elongation Factor G, domain II / Elongation Factor G, domain III / Translation elongation factor EFG/EF2, domain IV / Elongation factor G, domain IV / Elongation factor G, domain IV / Elongation factor G C-terminus / Double stranded RNA-binding domain (dsRBD) profile. / Elongation factor EFG, domain V-like / Elongation factor G C-terminus / Double-stranded RNA-binding domain / EF-G domain III/V-like / : / Tr-type G domain, conserved site / Translational (tr)-type guanine nucleotide-binding (G) domain signature. / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Beta-grasp domain superfamily / NTF2-like domain superfamily / Translation elongation factor EFTu-like, domain 2 / Elongation factor Tu domain 2 / NUDIX hydrolase-like domain superfamily / Translational (tr)-type GTP-binding domain / Elongation factor Tu GTP binding domain / Translational (tr)-type guanine nucleotide-binding (G) domain profile. / Ribosomal protein L10-like domain superfamily / Ribosomal protein L10P / Ribosomal protein L10 / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily
Similarity search - Domain/homology
Large ribosomal subunit protein mL49 / Large ribosomal subunit protein uL3m / Large ribosomal subunit protein mL62 / Mitochondrial ribosomal protein L18 / Large ribosomal subunit protein bL28m / 39S ribosomal protein L20, mitochondrial isoform 1 / Large ribosomal subunit protein bL33m / Large ribosomal subunit protein mL64 / 39S ribosomal protein L23, mitochondrial / Large ribosomal subunit protein bL34m ...Large ribosomal subunit protein mL49 / Large ribosomal subunit protein uL3m / Large ribosomal subunit protein mL62 / Mitochondrial ribosomal protein L18 / Large ribosomal subunit protein bL28m / 39S ribosomal protein L20, mitochondrial isoform 1 / Large ribosomal subunit protein bL33m / Large ribosomal subunit protein mL64 / 39S ribosomal protein L23, mitochondrial / Large ribosomal subunit protein bL34m / Ribosomal protein / Mitochondrial ribosomal protein L12 / Mitochondrial ribosomal protein L41 / Large ribosomal subunit protein uL4m / Large ribosomal subunit protein mL51 / Large ribosomal subunit protein mL37 / Large ribosomal subunit protein uL14m / Mitochondrial ribosomal protein L57 / Large ribosomal subunit protein mL42 / Large ribosomal subunit protein mL53 / 39S ribosomal protein L48, mitochondrial / 39S ribosomal protein S30, mitochondrial / Large ribosomal subunit protein mL40 / Large ribosomal subunit protein uL24m / Large ribosomal subunit protein uL16m / Large ribosomal subunit protein bL17m / Large ribosomal subunit protein uL22m / Mitochondrial ribosomal protein S18A / Mitochondrial ribosomal protein L2 / 39S ribosomal protein L15, mitochondrial / 39S ribosomal protein L27, mitochondrial / Large ribosomal subunit protein uL11m / Large ribosomal subunit protein mL38 / Large ribosomal subunit protein uL10m / Large ribosomal subunit protein bL21m / Large ribosomal subunit protein mL43 / Large ribosomal subunit protein mL52 / Large ribosomal subunit protein uL29m / Mitochondrial ribosomal protein L39 / Large ribosomal subunit protein mL44 / Large ribosomal subunit protein mL46 / Large ribosomal subunit protein uL30m / Large ribosomal subunit protein bL35m / Large ribosomal subunit protein mL50 / Mitochondrial ribosomal protein L55 / Large ribosomal subunit protein mL45 / Large ribosomal subunit protein mL54 / Large ribosomal subunit protein bL19m / Large ribosomal subunit protein bL9m / Large ribosomal subunit protein bL32m / 39S ribosomal protein L13, mitochondrial / Ribosome-releasing factor 2, mitochondrial / Ribosome-recycling factor, mitochondrial
Similarity search - Component
Biological speciesHomo sapiens (human) / Sus scrofa (pig) / Pig (pig)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsKummer E / Schubert K / Ban N
Funding support Switzerland, 2 items
OrganizationGrant numberCountry
European Molecular Biology Organization (EMBO) Switzerland
Swiss National Science Foundation Switzerland
CitationJournal: Mol Cell / Year: 2021
Title: Structural basis of translation termination, rescue, and recycling in mammalian mitochondria.
Authors: Eva Kummer / Katharina Noel Schubert / Tanja Schoenhut / Alain Scaiola / Nenad Ban /
Abstract: The mitochondrial translation system originates from a bacterial ancestor but has substantially diverged in the course of evolution. Here, we use single-particle cryo-electron microscopy (cryo-EM) as ...The mitochondrial translation system originates from a bacterial ancestor but has substantially diverged in the course of evolution. Here, we use single-particle cryo-electron microscopy (cryo-EM) as a screening tool to identify mitochondrial translation termination mechanisms and to describe them in molecular detail. We show how mitochondrial release factor 1a releases the nascent chain from the ribosome when it encounters the canonical stop codons UAA and UAG. Furthermore, we define how the peptidyl-tRNA hydrolase ICT1 acts as a rescue factor on mitoribosomes that have stalled on truncated messages to recover them for protein synthesis. Finally, we present structural models detailing the process of mitochondrial ribosome recycling to explain how a dedicated elongation factor, mitochondrial EFG2 (mtEFG2), has specialized for cooperation with the mitochondrial ribosome recycling factor to dissociate the mitoribosomal subunits at the end of the translation process.
History
DepositionMar 7, 2021-
Header (metadata) releaseMay 5, 2021-
Map releaseMay 5, 2021-
UpdateDec 15, 2021-
Current statusDec 15, 2021Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.028
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.028
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7nsh
  • Surface level: 0.028
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_12567.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.087 Å
Density
Contour LevelBy AUTHOR: 0.028 / Movie #1: 0.028
Minimum - Maximum-0.14436242 - 0.283887
Average (Standard dev.)-0.0006301594 (±0.008191228)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 521.76 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0871.0871.087
M x/y/z480480480
origin x/y/z0.0000.0000.000
length x/y/z521.760521.760521.760
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS480480480
D min/max/mean-0.1440.284-0.001

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Supplemental data

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Mask #1

Fileemd_12567_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Additional map: postprocessed and masked map

Fileemd_12567_additional_1.map
Annotationpostprocessed and masked map
Projections & Slices
AxesZYX

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Additional map: 5x supersampled map

Fileemd_12567_additional_2.map
Annotation5x supersampled map
Projections & Slices
AxesZYX

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Additional map: full map from 3D gold-standard refinement

Fileemd_12567_additional_3.map
Annotationfull map from 3D gold-standard refinement
Projections & Slices
AxesZYX

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Half map: #2

Fileemd_12567_half_map_1.map
Projections & Slices
AxesZYX

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Half map: #1

Fileemd_12567_half_map_2.map
Projections & Slices
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Sample components

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Entire : 39S mammalian mitochondrial large ribosomal subunit with mtRRF (p...

EntireName: 39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2
Components
  • Complex: 39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2
    • Complex: mtRRF (post) and mtEFG2
      • Protein or peptide: Ribosome-releasing factor 2, mitochondrial
      • Protein or peptide: Ribosome-recycling factor, mitochondrial
    • Complex: 39S mammalian mitochondrial large ribosomal subunit
      • Protein or peptide: Mitochondrial ribosomal protein L27
      • Protein or peptide: uL24m
      • Protein or peptide: Mitochondrial ribosomal protein L37
      • Protein or peptide: Mitochondrial ribosomal protein L35
      • Protein or peptide: Mitochondrial ribosomal protein L38
      • Protein or peptide: Mitochondrial ribosomal protein L39
      • Protein or peptide: Mitochondrial ribosomal protein L40
      • Protein or peptide: Mitochondrial ribosomal protein L41
      • Protein or peptide: mL42
      • Protein or peptide: Mitochondrial ribosomal protein L43
      • Protein or peptide: mL44
      • Protein or peptide: Mitochondrial ribosomal protein L45
      • Protein or peptide: Mitochondrial ribosomal protein L46
      • Protein or peptide: 39S ribosomal protein L48, mitochondrialRibosome
      • Protein or peptide: Mrpl34
      • Protein or peptide: Mitochondrial ribosomal protein L50
      • Protein or peptide: Mitochondrial ribosomal protein L51
      • Protein or peptide: mL52
      • Protein or peptide: mL53
      • Protein or peptide: mL54
      • Protein or peptide: Mitochondrial ribosomal protein L57
      • Protein or peptide: mL62 (ICT1)
      • Protein or peptide: mL64Microsoft Macro Assembler
      • Protein or peptide: 39S ribosomal protein S30, mitochondrialRibosome
      • Protein or peptide: Mitochondrial ribosomal protein S18A
      • Protein or peptide: Mitochondrial ribosomal protein L12
      • Protein or peptide: Mitochondrial ribosomal protein L28
      • Protein or peptide: Ribosomal protein
      • RNA: 16S rRNA
      • Protein or peptide: Mitochondrial ribosomal protein L47
      • Protein or peptide: uL30m
      • Protein or peptide: bL31m
      • Protein or peptide: bL32m
      • Protein or peptide: bL33m
      • Protein or peptide: Mitochondrial ribosomal protein L34
      • RNA: CP tRNAPhe
      • Protein or peptide: uL2m
      • Protein or peptide: uL16m
      • Protein or peptide: ICT1
      • Protein or peptide: Mitochondrial ribosomal protein L4
      • Protein or peptide: Mitochondrial ribosomal protein L9
      • Protein or peptide: Mitochondrial ribosomal protein L10
      • Protein or peptide: Mitochondrial ribosomal protein L11
      • Protein or peptide: uL13m
      • Protein or peptide: uL14m
      • Protein or peptide: uL15m
      • Protein or peptide: bL17m
      • Protein or peptide: Mitochondrial ribosomal protein L18
      • Protein or peptide: Mitochondrial ribosomal protein L19
      • Protein or peptide: Mitochondrial ribosomal protein L20
      • Protein or peptide: Mitochondrial ribosomal protein L21
      • Protein or peptide: uL22m
      • Protein or peptide: uL23m
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: GUANOSINE-5'-MONOPHOSPHATE
  • Ligand: SPERMINE
  • Ligand: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
  • Ligand: water

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Supramolecule #1: 39S mammalian mitochondrial large ribosomal subunit with mtRRF (p...

SupramoleculeName: 39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#55

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Supramolecule #3: mtRRF (post) and mtEFG2

SupramoleculeName: mtRRF (post) and mtEFG2 / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #37, #39
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Supramolecule #2: 39S mammalian mitochondrial large ribosomal subunit

SupramoleculeName: 39S mammalian mitochondrial large ribosomal subunit / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#36, #38, #40-#55
Source (natural)Organism: Sus scrofa (pig)

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Macromolecule #1: Mitochondrial ribosomal protein L27

MacromoleculeName: Mitochondrial ribosomal protein L27 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 15.943688 KDa
SequenceString:
MALAVLALRT RAAVTALLSP PQAAALAVRY ASKKTGGSSK NLGGKSPGKR FGIKKMEGHY VHAGNILATQ RHFRWHPGAH VGLGKNKCL YALEEGVVRY TKEVYVPNPS NSEAVDLVTR LPQGAVLYKT FVHVVPAKPE GTFKLVAML

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Macromolecule #2: uL24m

MacromoleculeName: uL24m / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 24.893529 KDa
SequenceString: MRLSALLALA SKVTLPPNYR YGMSRPGSLS DKRKNPPGTR RRRVPVEPIS DEDWHLFCGD KVEILEGKDA GKQGKVVQVI RQRNWVVVE GLNTHYRYVG KTVDYRGTMI PSEAPLLHNQ VKLVDPTDRK PTDVEWRFTE AGERVRVSTR SGRIIPKPEF P RADGIVPE ...String:
MRLSALLALA SKVTLPPNYR YGMSRPGSLS DKRKNPPGTR RRRVPVEPIS DEDWHLFCGD KVEILEGKDA GKQGKVVQVI RQRNWVVVE GLNTHYRYVG KTVDYRGTMI PSEAPLLHNQ VKLVDPTDRK PTDVEWRFTE AGERVRVSTR SGRIIPKPEF P RADGIVPE TWIDGPKDTS VEDALEKTYV PRLKTLEEEV MEAMGIQETR RHKKVYWY

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Macromolecule #3: Mitochondrial ribosomal protein L37

MacromoleculeName: Mitochondrial ribosomal protein L37 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 47.917988 KDa
SequenceString: MALASGPARR ALARLGRLGL GGCGAPRRGA YEWGVRSTRK PEPPPLDRVY EIPGLEPITY AGKMHFMPGL ARPVFPPWDP GWTHPKFRR LPPLHEHPLY KDQACYVFHQ RCRLLEGVKQ ALWLTKTQLI EGLPEKVLRL ADDPRNHIEN QDERVLNAIS H ARLWHSTE ...String:
MALASGPARR ALARLGRLGL GGCGAPRRGA YEWGVRSTRK PEPPPLDRVY EIPGLEPITY AGKMHFMPGL ARPVFPPWDP GWTHPKFRR LPPLHEHPLY KDQACYVFHQ RCRLLEGVKQ ALWLTKTQLI EGLPEKVLRL ADDPRNHIEN QDERVLNAIS H ARLWHSTE DIPKRETYCP VIVDSLIQLC KSQILKHPSL ARRICAQNNT LSATWNRESI LLQVHGSSGA RLNAKDPLPP VA SQEEVEA TKNHVLETFY PISPTMGLQE CNVYDVNDDT GFQEGYPYPC PHTLYFLESA NLRPRRFQPD QLRAKMILFA FGS ALAQAR LLYGNDSKVL EQPVVVQSVG TDGRLFQFLV LQLNTTDLAS DEGVKNLAWV DSDQLLYQHF WCLPVIKKKV VVEP VGPIG FQPETFRKFL ALYLHGAV

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Macromolecule #4: Mitochondrial ribosomal protein L35

MacromoleculeName: Mitochondrial ribosomal protein L35 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 21.548543 KDa
SequenceString:
MAASAFAGAV RAATGILRPL HILASSAYQN CAKNACLSSV LSSRHFSHIQ TSALLSAPRL ITSVRNLMCG PTAPVLNRVA PLLPHVLKP PVRTVTYFSS RKGKRKTVKA VIYRFLRLHS GLWLRRKAGY KKKLWKKTAA RKRRLREFVF CNKTQSKLLD K MTTSFWKR RNWYADDPYQ KYQDRTNLKV

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Macromolecule #5: Mitochondrial ribosomal protein L38

MacromoleculeName: Mitochondrial ribosomal protein L38 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 44.656387 KDa
SequenceString: MAAPWWRAAL CASRRWRGFS TSAALSRRAA PLGPMPNEDI DVSDLERLKK YRSFDRYRRR AEQEARKPHW WRTYREHFGE ESGPKDRVD IGLPPPKVSR TQQLLERKQA LRELRANVEE ERAARLQTAR IPLEAVRAEW ERTCGPYHKQ RLAEYCGLYR D LFHGATFV ...String:
MAAPWWRAAL CASRRWRGFS TSAALSRRAA PLGPMPNEDI DVSDLERLKK YRSFDRYRRR AEQEARKPHW WRTYREHFGE ESGPKDRVD IGLPPPKVSR TQQLLERKQA LRELRANVEE ERAARLQTAR IPLEAVRAEW ERTCGPYHKQ RLAEYCGLYR D LFHGATFV PRVPLHVAYA VGEDDLMPVY HGNEVTPTEA AQAPEVTYEA DEGSLWTLLL TNLDGHLLEP DAEYVHWLVT NI PGNRVTE GQETCPYLPP FPARGSGFHR FAFLLFKQDK RIDFSGDTRP SPCYQLAQRT FHTFDFYKKH QDAMTPAGLA FFQ CRWDDS VTRVFHQLLD MREPVFEFVR PPPYHPKQKR FPHRQPLRYL DRYRDSHEPT YGIY

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Macromolecule #6: Mitochondrial ribosomal protein L39

MacromoleculeName: Mitochondrial ribosomal protein L39 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 38.523422 KDa
SequenceString: MAMGAWGLRL WRAVPRGEAG WRLIATSPAS QLSPTELIEM QNDLFNKEKN RQLSLTPRTE KIEVKHVGKT DPGTVFVMNK NISTPYSCA MHLSEWYCRK SILALVDGQP WDMYKPLTKS CEIKFLTFKD DDPGEVNKAY WRSCAMMMGC VIERAFKDEY V VSLVRAPE ...String:
MAMGAWGLRL WRAVPRGEAG WRLIATSPAS QLSPTELIEM QNDLFNKEKN RQLSLTPRTE KIEVKHVGKT DPGTVFVMNK NISTPYSCA MHLSEWYCRK SILALVDGQP WDMYKPLTKS CEIKFLTFKD DDPGEVNKAY WRSCAMMMGC VIERAFKDEY V VSLVRAPE VPVIAGAFCY DVVLDKRLDE WMPTKENLHS FTKDARALIY KDLPFETLEV EAKVALEIFQ HNKYKLDFIE EK ASQNPER IVKLHRFGDF IDVSEGPLIP RTSICFQYEV SAVHNLQTQS SLVRRFQGLS LPVHLRAHFT IWNKLLERSR KMV TEDKTK PTEESAST

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Macromolecule #7: Mitochondrial ribosomal protein L40

MacromoleculeName: Mitochondrial ribosomal protein L40 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 24.075658 KDa
SequenceString: MAAAALGAAS RTLRPASRLV GAWPTQTRDA HERGSLFSFW GLVPMRAEPL RKKKKVDPKK DQAAKDRLKK RIRRLEKASQ ELIPIEDFI TPVKFLNKER QRPPVELPFE ESERRALLLK RWSLYKQREH EMERSAIRSL LEAQEEALQE LRLSSPELHA E ATKRDPSL ...String:
MAAAALGAAS RTLRPASRLV GAWPTQTRDA HERGSLFSFW GLVPMRAEPL RKKKKVDPKK DQAAKDRLKK RIRRLEKASQ ELIPIEDFI TPVKFLNKER QRPPVELPFE ESERRALLLK RWSLYKQREH EMERSAIRSL LEAQEEALQE LRLSSPELHA E ATKRDPSL FPFERQGPDY TPPISDYQPP EGRYQDITKV YTQVEFKK

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Macromolecule #8: Mitochondrial ribosomal protein L41

MacromoleculeName: Mitochondrial ribosomal protein L41 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 15.073404 KDa
SequenceString:
MGLLSGAARA LVRGADRMSK WTSKRGPRTF CKGRGAKGTG FHGRDGKFVQ IKEMIPELVV PELAGFKLKP YVNYRAPEGT DTPLTAKQL FLETAAPAIE KDFKAGTFDP EHLEKYGFEP TQEGKLFQLY PKNFPR

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Macromolecule #9: mL42

MacromoleculeName: mL42 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 16.680178 KDa
SequenceString:
MALAAVKWVI SSRTILKHLF PIQNSASYCV CHKSTYSSLP DDYNCKVELA LTSDGRTIVC YHPSVDIPYE HTKPIPRPDP VHNIEETHD LVLKTRLEEK GEHLEQGPMI EQLSKMFFTT KHRWYPRGQY HRRRRKPNPP KDR

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Macromolecule #10: Mitochondrial ribosomal protein L43

MacromoleculeName: Mitochondrial ribosomal protein L43 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 17.728109 KDa
SequenceString:
MTARGTASRF LTSVLHNGLG RYVQQLQRLS FSLSRDAPSS RGAREFVERE VTDFARRNPG VVIYVNPRPC CVPRVVAEYL NGAVREESI HCKSVEEIAA LVQKLADQSG LDVIRIRKPF HTDSPSIQGQ WHPFTNKPTT LGGLRPREVQ DPAPAQVQAQ

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Macromolecule #11: mL44

MacromoleculeName: mL44 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 37.527195 KDa
SequenceString: MASGLVRLLQ WGPRRLLAPA APTLAPPVRG AKKGFRAAYR FQKELERWRL LRCPPPPVRR SEKPNWDYHA EIQAFGHRLQ ETFSLDLLK TAFVNSCYIK SEEAKRQKLG IDKEAALLNL KDNQELSEQG ISFSQTCLTQ FFEDAFPDLP TEGVTSLVDF L TSEEVVCH ...String:
MASGLVRLLQ WGPRRLLAPA APTLAPPVRG AKKGFRAAYR FQKELERWRL LRCPPPPVRR SEKPNWDYHA EIQAFGHRLQ ETFSLDLLK TAFVNSCYIK SEEAKRQKLG IDKEAALLNL KDNQELSEQG ISFSQTCLTQ FFEDAFPDLP TEGVTSLVDF L TSEEVVCH VARNLAVEQL ALSAEFPVPP PVLRQTFFAV IGALLQSSGP ERTALFIRDF LITQMTGKEL FEMWTITNPM GL LVEELKK RKISAPESRL TRQSGSTTAL PVYFVGLYCD RKLIAEGPGE TVLVAEEEAA RVALRKLFGF TENRRPWDYS KPK EHVRAE KTITAS

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Macromolecule #12: Mitochondrial ribosomal protein L45

MacromoleculeName: Mitochondrial ribosomal protein L45 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 35.331805 KDa
SequenceString: MAAPVTRGLS CLPRVLGWWS RQPVLVTQST AVVPVRTKKR FTPPTYQPKY KSEKEFVEHA RKAGLVIPHE RLERPIHLAC TAGIFDAYV PPEGDARISS LSKEGLAQRA ERLKKNVASQ LSIRKIRESD PNFKIKDFPE KAKDIFIEAH LCLNNSDHDR L HTLVTENC ...String:
MAAPVTRGLS CLPRVLGWWS RQPVLVTQST AVVPVRTKKR FTPPTYQPKY KSEKEFVEHA RKAGLVIPHE RLERPIHLAC TAGIFDAYV PPEGDARISS LSKEGLAQRA ERLKKNVASQ LSIRKIRESD PNFKIKDFPE KAKDIFIEAH LCLNNSDHDR L HTLVTENC FPDMVWDIRY KTVRWSFVES LEPPQVVQVR CSSLMNQGNI YGQVTVRMHT RQTLAIYDRF GRLMYGQEDV PR DVLEYVV FEKHLVDPYG SWRMHGKIIP PWAPPKQPIL KTVMIPGPQL KPWEEFEEPQ GEVHKPQPA

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Macromolecule #13: Mitochondrial ribosomal protein L46

MacromoleculeName: Mitochondrial ribosomal protein L46 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 32.215998 KDa
SequenceString: MAAPVRRTML RVVRGWRRFE GPWAHSLGSR NLALAVAPSS SSSPWRLLGA LCLQRPPLVT KPLTPLQEEM ADLLQQIEIE RSLYSDHEL RALDEAQQLA KKKSDLYEEE DEQNILLAQD LEDMWEQKFL HFKLGARLTE ADKKDDRTSL HRKLDRNLIL L VREKLGDQ ...String:
MAAPVRRTML RVVRGWRRFE GPWAHSLGSR NLALAVAPSS SSSPWRLLGA LCLQRPPLVT KPLTPLQEEM ADLLQQIEIE RSLYSDHEL RALDEAQQLA KKKSDLYEEE DEQNILLAQD LEDMWEQKFL HFKLGARLTE ADKKDDRTSL HRKLDRNLIL L VREKLGDQ DIWMLPQSDW QPGETLRQTA ERTLATLSEN NMEAKFLGNA PCGHYKFKFP QAMRTETSLG AKVFFFKALL LT GDFSQAG KKGHHVWVSK EELGDYLKPK YLAQVRRFLL DL

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Macromolecule #14: 39S ribosomal protein L48, mitochondrial

MacromoleculeName: 39S ribosomal protein L48, mitochondrial / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 29.942523 KDa
SequenceString: MSEAAGTKVL RELNASRSDI VTGPDQGRSV SIFRPPGIAA AWADLQLARL HTTRQQRMNG ALGKALCLRN DTVLKQALSL IRVRASGES PICSAGGILL STSRHYRSKP THGIGRYKHL VKAQEPKKKK GKVEVRPINL GTDYEYGVLN IHLIAYDMAL A ESYAQYVH ...String:
MSEAAGTKVL RELNASRSDI VTGPDQGRSV SIFRPPGIAA AWADLQLARL HTTRQQRMNG ALGKALCLRN DTVLKQALSL IRVRASGES PICSAGGILL STSRHYRSKP THGIGRYKHL VKAQEPKKKK GKVEVRPINL GTDYEYGVLN IHLIAYDMAL A ESYAQYVH NLCNHLAIKV EESYAMPTKT MEVLQLQEQG SKMFLDAVLT THERVVQISG LSATFAEIFL EIIHSNLPEG VK LSVREHT EEDFKGRFKA RPELEELLAK LN

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Macromolecule #15: Mrpl34

MacromoleculeName: Mrpl34 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 18.957779 KDa
SequenceString:
MAATVLCGVL RAWRTGVPLG CGLRRLSQTQ GTPEYPSFVE SVDEYHFVER LLPPASIPRP PKHEHYPTPS GWQPPRDPAP SLPYFVRRS RMHNIPVYRD ITHGNRQMTV IRKVEGDIWA LQKDVEDFLS PLLGKTPVTQ VNEVTGTLRV KGYFDQQLKA W LLEKGF

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Macromolecule #16: Mitochondrial ribosomal protein L50

MacromoleculeName: Mitochondrial ribosomal protein L50 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 22.785357 KDa
SequenceString: MVRLRGKREC LTLSLRLIGW SWRVTAGGGR CFVSKFSSKM AARWVSGLAR RSLTCAVSGA PRREFWSPFR KEKQPVVAET VEEVKKEPI LVCPPIQSRT YIPPEDLQSR LESHVKEVFG SSVPSNWQDI SLEDVHLKFS FLARLANDLR HAVPNSRLHQ M CRVRDVLD ...String:
MVRLRGKREC LTLSLRLIGW SWRVTAGGGR CFVSKFSSKM AARWVSGLAR RSLTCAVSGA PRREFWSPFR KEKQPVVAET VEEVKKEPI LVCPPIQSRT YIPPEDLQSR LESHVKEVFG SSVPSNWQDI SLEDVHLKFS FLARLANDLR HAVPNSRLHQ M CRVRDVLD FYNVPVQDRS KFDELIASNL PHNLKITWGY

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Macromolecule #17: Mitochondrial ribosomal protein L51

MacromoleculeName: Mitochondrial ribosomal protein L51 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 15.249048 KDa
SequenceString:
MAGSLSWVTG RGLWGRLPLT CRSFSLGIPR LFHVRVTLPP RKVVDRWNEK RAMFGVYDNI GILGNFEKHP KELIKGPIWL RGWKGNELQ RCIRKKRMVG NRMFIDDLHN LNKRISYLYK HFNRHGKYR

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Macromolecule #18: mL52

MacromoleculeName: mL52 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 13.813705 KDa
SequenceString:
MAAWGILLST GVRRLHCGTA AQAGSQWRLQ QGFAANPSGY GPLTELPDWS YADGRPAPPM KGQLRRKAQR EKFARRVVLL SQEMDAGLQ AWQLRQQEKL QEEEGKQKNA LKSKGALLQN PQPSQ

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Macromolecule #19: mL53

MacromoleculeName: mL53 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 12.015747 KDa
SequenceString:
MAAALARLGL RAVKQVRVQF CPFEKNVEST RTFLQAVSSE KVRCTNLNCS VIADVRHDGS EPCVDVLFGD GHRLIMRGAH LTAQEMLTA FASHIQARGA AGSGDKPSAS TGR

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Macromolecule #20: mL54

MacromoleculeName: mL54 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 15.957357 KDa
SequenceString:
MAARRLFGAA RSWAAWRAWE LSDAAVSGRL HVRNYAKRPV IKGGKGGKGA VVGEALKDPE VCTDPFRLTT HAMGVNIYKE GQDVVLKPD SEYPEWLFEM NVGPPKKLEE LDPETREYWR LLRKHNIWRH NRLSKNRKF

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Macromolecule #21: Mitochondrial ribosomal protein L57

MacromoleculeName: Mitochondrial ribosomal protein L57 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 12.001915 KDa
SequenceString:
MFLTALLRRN RIPGRQWIGK HRRPRPVSAQ AKQNMIRRLE TEAENQYWLS RPFLTAEQER GHAAVRRAAA FQALKAAQAA RFPAHRRLE EQLGHLLVTR KWS

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Macromolecule #22: mL62 (ICT1)

MacromoleculeName: mL62 (ICT1) / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 23.545873 KDa
SequenceString: MAAARCLRWG LNRAGAWLLP SPTRYPRRAL HKQVEGTEFQ SIYSLDKLYP ESRGSDTAWR LPDDAKQAND IPVDRLTISY CRSSGPGGQ NVNKVNSKAE VRFHLASADW IAEPVRLKLA VKHKNRINRS GELILTSECS RYQFRNLADC LQKLRDMIAE A SQPAKEPS ...String:
MAAARCLRWG LNRAGAWLLP SPTRYPRRAL HKQVEGTEFQ SIYSLDKLYP ESRGSDTAWR LPDDAKQAND IPVDRLTISY CRSSGPGGQ NVNKVNSKAE VRFHLASADW IAEPVRLKLA VKHKNRINRS GELILTSECS RYQFRNLADC LQKLRDMIAE A SQPAKEPS REDAELRRSR IENMNRERLR KKRISSAIKT SRRVDVD

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Macromolecule #23: mL64

MacromoleculeName: mL64 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 25.815031 KDa
SequenceString: MAAPVQQART LLRLVTTLGQ GSRGYRARPP PRRSQEPRWP DPDDPLTPRW QLSPRYAAKQ FARHGAASGV AAGSLWPSQE QLRELEAEE REWYPSLAAM QESLRVQQLA EEQKRQAREQ LIEECMAKMP QMIENWRQQQ QERREKEQAD KDRRARLQAE A QERLGYHV ...String:
MAAPVQQART LLRLVTTLGQ GSRGYRARPP PRRSQEPRWP DPDDPLTPRW QLSPRYAAKQ FARHGAASGV AAGSLWPSQE QLRELEAEE REWYPSLAAM QESLRVQQLA EEQKRQAREQ LIEECMAKMP QMIENWRQQQ QERREKEQAD KDRRARLQAE A QERLGYHV DPRSARFQEL LQDMEKQHRK RLKEEKQRKK KEARAAAMAA AAAQDPADSE TPSS

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Macromolecule #24: 39S ribosomal protein S30, mitochondrial

MacromoleculeName: 39S ribosomal protein S30, mitochondrial / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 49.100504 KDa
SequenceString: MAAARCWRFP LRGAGLSLHT AAKAAVTAPE VTGPDVPATP VARYPPIVAS LTAKSKAARQ RRVEQWQATV HAAKSVDEKL RILTKMQFM KYVVYPQTFA LNADNWYQSF TKTVFLSGLP PTPAKLEPEP TLDITALREA VCDCLLQEHF FLRRKKRAPV I QDREAIAS ...String:
MAAARCWRFP LRGAGLSLHT AAKAAVTAPE VTGPDVPATP VARYPPIVAS LTAKSKAARQ RRVEQWQATV HAAKSVDEKL RILTKMQFM KYVVYPQTFA LNADNWYQSF TKTVFLSGLP PTPAKLEPEP TLDITALREA VCDCLLQEHF FLRRKKRAPV I QDREAIAS PFLDQLVASL TGLLSVHNPV LAAAALDCKR PVHFFWLRGE EIIPRGHRKG RVDALRYQIN DKPHNQIRIS RQ LPEFVPL DYSIPIEVPV MSCKPDKLPL FKRQYENTIF IGSKTADPLC YGHTQFHLLP DKLKREKLLK QNCADQIEVV FRA NAIASL FAWTGAQAMY QGFWSEADVT RPFVSQGVIT DGKYFSFFCY QLNTLALTAQ ADQNNPRKNI CWGTQSKPLY ETIE DNNVK GFNDDVLLQL VQFLLNRPKE DKSQLLEN

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Macromolecule #25: Mitochondrial ribosomal protein S18A

MacromoleculeName: Mitochondrial ribosomal protein S18A / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 22.273084 KDa
SequenceString: MVGLNVLVSG CGRLLRGLLA GPAATSWARP PSRGFREVVE ILDGKTTIIE GRITETPQES PNPPNPTGQC PICRWNLKHK YNYEDVLLL SQFIRPHGGM LPRRITGLCQ EEHLKIEECV KMAHRAGLLP NHRPKLPEGF VPKSKPRLNR YLTRWSPRSV K PIYNKGHR ...String:
MVGLNVLVSG CGRLLRGLLA GPAATSWARP PSRGFREVVE ILDGKTTIIE GRITETPQES PNPPNPTGQC PICRWNLKHK YNYEDVLLL SQFIRPHGGM LPRRITGLCQ EEHLKIEECV KMAHRAGLLP NHRPKLPEGF VPKSKPRLNR YLTRWSPRSV K PIYNKGHR WNKVRMPVGS PLLKDNVSYS GRPLLLYH

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Macromolecule #26: Mitochondrial ribosomal protein L12

MacromoleculeName: Mitochondrial ribosomal protein L12 / type: protein_or_peptide / ID: 26 / Number of copies: 7 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 21.268975 KDa
SequenceString: MLPAAASSLW GPCFGLRAAA LRVARHQGPR LCGVRLMRCS SHRKGEALAG APLDNAPKEY PPKIQQLVQD IASLTLLEIS DLNELLKKT LKIQDVGFMP MGAVAPGAPP AAAAPEAAEE DLPKRKEQTH FTVRLTEAKP VDKVKLIKEI KSHIQGINLV Q AKKLVESL ...String:
MLPAAASSLW GPCFGLRAAA LRVARHQGPR LCGVRLMRCS SHRKGEALAG APLDNAPKEY PPKIQQLVQD IASLTLLEIS DLNELLKKT LKIQDVGFMP MGAVAPGAPP AAAAPEAAEE DLPKRKEQTH FTVRLTEAKP VDKVKLIKEI KSHIQGINLV Q AKKLVESL PQEIKANVPK AEAEKIKAAL EAVGGTVVLE

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Macromolecule #27: Mitochondrial ribosomal protein L28

MacromoleculeName: Mitochondrial ribosomal protein L28 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 30.148953 KDa
SequenceString: MPLHKVPVGL WKQLRLWEGI YSRLPRHYLR SLEEARTPTP VHYRPHGAKF KINPKNWQRE RVEDVPIPVH YPPESQLGLW GGEGWVLGH RYVNNDKLSK RVRKVWKPQL FQRELYSEIL DKRFTVTVTM RTLDLIDQAC GFDFYILKTP KEDLCSKFGM D LKRGMLLR ...String:
MPLHKVPVGL WKQLRLWEGI YSRLPRHYLR SLEEARTPTP VHYRPHGAKF KINPKNWQRE RVEDVPIPVH YPPESQLGLW GGEGWVLGH RYVNNDKLSK RVRKVWKPQL FQRELYSEIL DKRFTVTVTM RTLDLIDQAC GFDFYILKTP KEDLCSKFGM D LKRGMLLR LARQDPQLHP DDPARRAAIY DRYKAFVIPE AEAEWVGLTL DEAVEKQRLL EEKDPIPLFK IFVEELLGQL QQ QALSEPA VVQTRASRK

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Macromolecule #28: Ribosomal protein

MacromoleculeName: Ribosomal protein / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 10.841016 KDa
SequenceString:
MATAFLRTVL SAVGPLLHLG GRPLSTFAAG PPRAALAVGA QPSPAAALLS ARPLLGPQPA LGFKTKGVLK KRCRDCYLVK RRGRWFIYC KTNPKHKQRQ M

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Macromolecule #30: Mitochondrial ribosomal protein L47

MacromoleculeName: Mitochondrial ribosomal protein L47 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 30.085088 KDa
SequenceString: MAAAGLAVFC RRVSAALKAC RLLIRPQAPP STSCRFSPSL LPKNTPNVTS FHQFRIFHTT FSRRGLEEFF DDPKNWGEEK VKSGASWTC QQLRNKSNED LHKLWYVLLK ERNMLLTLEQ EAKRQRLPMP SPERLEKVVD SMDALDKVVQ EREDALRLLQ T GQEKARPG ...String:
MAAAGLAVFC RRVSAALKAC RLLIRPQAPP STSCRFSPSL LPKNTPNVTS FHQFRIFHTT FSRRGLEEFF DDPKNWGEEK VKSGASWTC QQLRNKSNED LHKLWYVLLK ERNMLLTLEQ EAKRQRLPMP SPERLEKVVD SMDALDKVVQ EREDALRLLQ T GQEKARPG AWRRDIFGRI IWHKFKQWPI PWYLNKKYNR KRFFAMPYVE RFVRMRIEKQ ARIKARKRSL ERKKEKFLQE KF PHLSETQ KSSHV

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Macromolecule #31: uL30m

MacromoleculeName: uL30m / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 18.9211 KDa
SequenceString:
HPPRQDFLVR RQPHRNRTVT KGMESLICTD WIRHKFTRSR IPDKVFQPSP EDHEKYGGDP QYPHKLHIVT RIKSTKRRPY WEKDIIKML GLEKAHTPQV HKNIPSVNAK LKVVKHLIRI KPLKLPQGLP TEEDMANTCL KSNGELVVRW LLNPANQEAR K S

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Macromolecule #32: bL31m

MacromoleculeName: bL31m / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 15.156537 KDa
SequenceString:
MAAKGSLLRL LWQRVVTGAA PESCRHLYTS SWRADCNRAL LTRLHRQTYA RLYPVLLVKQ DGSTIHIRYR EPRRMLTMPV DLDSLSPEE RRARFRKREA KFKEKKEEPE LSDDFDVEQY KRFWTKK

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Macromolecule #33: bL32m

MacromoleculeName: bL32m / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 21.53793 KDa
SequenceString:
MASAMLVLVV PPWPAARGLL RNWWEQLQRK LQHNRLGLPL HPWGPALAVQ GPAICTEPAN DTNGSKAISS LLDSVFWMAA PKNRRSIEV NRCRRRNPQK LIKVKNNIDV CPECGHLKQK HILCGYCYEK VRKETAEIRR QMGKQEGGPF RAPTTETVVL Y SGETPSEQ DQGKRIIERE RKRPSWFTQN

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Macromolecule #34: bL33m

MacromoleculeName: bL33m / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 7.508097 KDa
SequenceString:
MFLSAVTFAK SKSKTILVKM MSQAGTGFSF NTKRSRLREK LTLLHYDPVV KKKVLFVEQK KIRSL

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Macromolecule #35: Mitochondrial ribosomal protein L34

MacromoleculeName: Mitochondrial ribosomal protein L34 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 10.871729 KDa
SequenceString:
MAFLARSVGR LLDPVSRSAA LVGGRWLQPQ AWLGFPDTWG LPAMQQTRGK ARGNEYQPSN IKRKHKHGWV RRLRTPTGVQ VILRRMHKG RKSLSH

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Macromolecule #37: Ribosome-releasing factor 2, mitochondrial

MacromoleculeName: Ribosome-releasing factor 2, mitochondrial / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 83.848656 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MHHHHHHENL YFQSGGSGSG CSSLPGLIGN DIKSLHSIIN PPIAKIRNIG IMAHIDAGKT TTTERILYYS GYTRSLGDVD DGDTVTDFM AQERERGITI QSAAVTFDWK GYRVNLIDTP GHVDFTLEVE RCLRVLDGAV AVFDASAGVE AQTLTVWRQA D KHNIPRIC ...String:
MHHHHHHENL YFQSGGSGSG CSSLPGLIGN DIKSLHSIIN PPIAKIRNIG IMAHIDAGKT TTTERILYYS GYTRSLGDVD DGDTVTDFM AQERERGITI QSAAVTFDWK GYRVNLIDTP GHVDFTLEVE RCLRVLDGAV AVFDASAGVE AQTLTVWRQA D KHNIPRIC FLNKMDKTGA SFKYAVESIR EKLKAKPLLL QLPIGEAKTF KGVVDVVMKE KLLWNCNSND GKDFERKPLL EM NDPELLK ETTEARNALI EQVADLDDEF ADLVLEEFSE NFDLLPAEKL QTAIHRVTLA QTAVPVLCGS ALKNKGIQPL LDA VTMYLP SPEERNYEFL QWYKDDLCAL AFKVLHDKQR GPLVFMRIYS GTIKPQLAIH NINGNCTERI SRLLLPFADQ HVEI PSLTA GNIALTVGLK HTATGDTIVS SKSSALAAAR RAEREGEKKH RQNNEAERLL LAGVEIPEPV FFCTIEPPSL SKQPD LEHA LKCLQREDPS LKVRLDPDSG QTVLCGMGEL HIEIIHDRIK REYGLETYLG PLQVAYRETI LNSVRATDTL DRTLGD KRH LVTVEVEARP IETSSVMPVI EFEYAESINE GLLKVSQEAI ENGIHSACLQ GPLLGSPIQD VAITLHSLTI HPGTSTT MI SACVSRCVQK ALKKADKQVL EPLMNLEVTV ARDYLSPVLA DLAQRRGNIQ EIQTRQDNKV VIGFVPLAEI MGYSTVLR T LTSGSATFAL ELSTYQAMNP QDQNTLLNRR SGLT

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Macromolecule #38: uL2m

MacromoleculeName: uL2m / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 33.270934 KDa
SequenceString: MALRVLTRAL SSLSLTPRIA VAPGLNLLPA VQVTNNVLLT LPSGLMSLPC RPILTSVALS ATSVSWKSRT KYTVMPVKMR KSGGRNHTG QIQVHGIGGG HKQRYRMIDF LRFRPEHESK PGPFEEKVIA VRYDPCRSAD IALVAGGNRK RWIIATENMK A GDTVLNSD ...String:
MALRVLTRAL SSLSLTPRIA VAPGLNLLPA VQVTNNVLLT LPSGLMSLPC RPILTSVALS ATSVSWKSRT KYTVMPVKMR KSGGRNHTG QIQVHGIGGG HKQRYRMIDF LRFRPEHESK PGPFEEKVIA VRYDPCRSAD IALVAGGNRK RWIIATENMK A GDTVLNSD HIGRMAVAAR EGDAHPLGAL PVGTLINNVE SEPGRGAQYI RAAGTCGVLL RKVNGTAIIQ LPSKRQMQVL ET CIATVGR VSNVDHNKRV IGKAGRNRWL GKRPNSGLWQ RKGGWAGRKI RPLPPMKSYV KLPSAAAQS

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Macromolecule #39: Ribosome-recycling factor, mitochondrial

MacromoleculeName: Ribosome-recycling factor, mitochondrial / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 28.689992 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MHHHHHHENL YFQSGGSGSG VSEVTLKTVH ERQHGHRQYM AYSAVPVRHF ATKKAKAKGK GQSQTRVNIN AALVEDIINL EEVNEEMKS VIEALKDNFN KTLNIRTSPG SLDKIAVVTA DGKLALNQIS QISMKSPQLI LVNMASFPEC TAAAIKAIRE S GMNLNPEV ...String:
MHHHHHHENL YFQSGGSGSG VSEVTLKTVH ERQHGHRQYM AYSAVPVRHF ATKKAKAKGK GQSQTRVNIN AALVEDIINL EEVNEEMKS VIEALKDNFN KTLNIRTSPG SLDKIAVVTA DGKLALNQIS QISMKSPQLI LVNMASFPEC TAAAIKAIRE S GMNLNPEV EGTLIRVPIP QVTREHREML VKLAKQNTNK AKDSLRKVRT NSMNKLKKSK DTVSEDTIRL IEKQISQMAD DT VAELDRH LAVKTKELLG

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Macromolecule #40: uL16m

MacromoleculeName: uL16m / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 28.680531 KDa
SequenceString: MWRLLARACA PLLRAPLSDS WAAPSATAGF KTLLPVPTFE DVAIPEKPKL RFVERVPLVP KVRRERKNLS DIRGPSTEAT EFTEGRFAI LALGGGYLHW GHFEMMRLTI NRSMDPKNMF ALWRVPAPFK PITRKGMGQR MGGGKGAIDH YVTPVKAGRL I VEMGGRCE ...String:
MWRLLARACA PLLRAPLSDS WAAPSATAGF KTLLPVPTFE DVAIPEKPKL RFVERVPLVP KVRRERKNLS DIRGPSTEAT EFTEGRFAI LALGGGYLHW GHFEMMRLTI NRSMDPKNMF ALWRVPAPFK PITRKGMGQR MGGGKGAIDH YVTPVKAGRL I VEMGGRCE FKEVQGFLDL VAHKLPFPAK AVSRETLEKM RKDQEERERN NQNPWTFERI ATANMLGIRK VLSPYDLTHK GR YWGKFYT PERV

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Macromolecule #41: ICT1

MacromoleculeName: ICT1 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 43.70827 KDa
SequenceString: MAPAALLPSW GQRGAAAGGG GKAWEGRRRN ALKAEWRGAQ RCSGRALLGL TMPGWGLLGR AGARVLGCGA DGLGASRGLG NRTDICLLV RSLHGKSVTW WDEHLSEENV PFVKQLVSDE NKAQLASKLC PLKDEPWPIH PWEPGSSRVG LIALKLGMMP L WTKDGQKH ...String:
MAPAALLPSW GQRGAAAGGG GKAWEGRRRN ALKAEWRGAQ RCSGRALLGL TMPGWGLLGR AGARVLGCGA DGLGASRGLG NRTDICLLV RSLHGKSVTW WDEHLSEENV PFVKQLVSDE NKAQLASKLC PLKDEPWPIH PWEPGSSRVG LIALKLGMMP L WTKDGQKH VVTLLQVQDC HVLKYTPKEN HNGRMAALTV GGKTVSHFHK SASILEFYQE LGLPPKQKVK IFNVTENAVI KP GTPLYAA HFRPGQYVDV TAKTIGKGFQ GVMRRWGFKG QPATHGQTKT HRRPGAISTG DVARVWPGTK MPGQLGNIDR TAF GLKVWR INTKHNIIYV NGSVPGHKNC LVKIKDSKLP AYKDFCKNLP FPTYFPDGDE EALPEDLYDE NVCQPGAPSI TFT

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Macromolecule #42: Mitochondrial ribosomal protein L4

MacromoleculeName: Mitochondrial ribosomal protein L4 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 33.251469 KDa
SequenceString: MLQLVRAGAR TWFRPSGCRG LNTLAEEAVQ QAEKPESVAS LGLQPPVLRK CELPVPAHRR PVQAWIESLR GYEQERVGLT ELHPDVFST APRLDILHQV AIWQKNFKRI SYAKTKTRAE VRGGGRKPWV QKGSGRARHG SIRSPIWRGG GVAHGPRGPT S YYYMLPMK ...String:
MLQLVRAGAR TWFRPSGCRG LNTLAEEAVQ QAEKPESVAS LGLQPPVLRK CELPVPAHRR PVQAWIESLR GYEQERVGLT ELHPDVFST APRLDILHQV AIWQKNFKRI SYAKTKTRAE VRGGGRKPWV QKGSGRARHG SIRSPIWRGG GVAHGPRGPT S YYYMLPMK VRVQGLKVAL TVKLAQDDLH IVDSLELPTA DPQYLIELAR YRRWGDSVLL VDLEHEDMPQ NVVAATSGLK TF NLVPAVG LNVHSMLKHQ TLVLTLPTVA FLEEKLLWHN SRYTPLYPFR LPYCDFP

+
Macromolecule #43: Mitochondrial ribosomal protein L9

MacromoleculeName: Mitochondrial ribosomal protein L9 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 30.373449 KDa
SequenceString: MAAAACTAAG GGLLRAGLER LLRGGVRALL RPRLEGVTPR SERAFSLSLS RSTVIVERWW KVPLAGEGRK PRLHRRHRVY KLVEDTKHR PKDNLELVLT QSVEELGVRG DLVSVKKSVG RNRLLPQGLA VYASPENKKL FEEEKLLRQE GKLEKLQTKA G EATVKFLR ...String:
MAAAACTAAG GGLLRAGLER LLRGGVRALL RPRLEGVTPR SERAFSLSLS RSTVIVERWW KVPLAGEGRK PRLHRRHRVY KLVEDTKHR PKDNLELVLT QSVEELGVRG DLVSVKKSVG RNRLLPQGLA VYASPENKKL FEEEKLLRQE GKLEKLQTKA G EATVKFLR RCHLEVGMKN NVKWELNPEI VARHFLRNLG VVVAPHALKL PEEPITQRGE YWCEVTVNGL DTVRVPMSVV NF ERPKTKR YKYWLAQQAA KGDVPTSSQM I

+
Macromolecule #44: Mitochondrial ribosomal protein L10

MacromoleculeName: Mitochondrial ribosomal protein L10 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 29.449689 KDa
SequenceString: MAAVVAGMLR GGLLPQAGRL PTFQTVRYGS KAVTRHRRVM HFERQKLMAV TEYIPPKPTI NPRCLPPPPT PPQEETGLVR LLRREIAAV FRDNRMIAVC QHVALSAEDK LLLRHQLRKH KILMKIFPNQ VLKPFLEESK YQNLLPLFVG HNLLLVSEEP K VKEMVRIL ...String:
MAAVVAGMLR GGLLPQAGRL PTFQTVRYGS KAVTRHRRVM HFERQKLMAV TEYIPPKPTI NPRCLPPPPT PPQEETGLVR LLRREIAAV FRDNRMIAVC QHVALSAEDK LLLRHQLRKH KILMKIFPNQ VLKPFLEESK YQNLLPLFVG HNLLLVSEEP K VKEMVRIL KGIPFLPLLG GCIDDTILSR QGFINYSKLP SLALVQGELV GGLTFLTAQT YSMLQHQPRQ LTALLDQYVK QQ QEGHPAV PASGQPEPPD PVPDP

+
Macromolecule #45: Mitochondrial ribosomal protein L11

MacromoleculeName: Mitochondrial ribosomal protein L11 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 20.809271 KDa
SequenceString: MSKLSRATRA LKKPEASGMI RAIVRAGQAR PGPPLGPILG QRGVSINQFC KEFNEKTKDI KEGIPLPTKI FVKPDRTFEI KIGQPTVSY FLKAAAGIEK GARHTGKEVA GLVTLKHVYE IARVKAQDDA FALQDVPLSS VVRSIIGSAR SLGIRVVKDL S SEELAAFQ ...String:
MSKLSRATRA LKKPEASGMI RAIVRAGQAR PGPPLGPILG QRGVSINQFC KEFNEKTKDI KEGIPLPTKI FVKPDRTFEI KIGQPTVSY FLKAAAGIEK GARHTGKEVA GLVTLKHVYE IARVKAQDDA FALQDVPLSS VVRSIIGSAR SLGIRVVKDL S SEELAAFQ KERALFLAAQ READLAAQAE AAKK

+
Macromolecule #46: uL13m

MacromoleculeName: uL13m / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 20.633801 KDa
SequenceString:
MSSFSKAPQQ WATFARVWYL LDGKMQPPGK LAAMASVKLQ GLHKPVYHQL SDCGDHVVIM NTRHIAFSGN KWEQKVYSSH TGYPGGFRQ VTAAQLHQKD PVAIVKLAIY GMLPKNLHRR TMMQRLHLFP DEDIPEDILK NLVEELPQPR KVPRRLDEYT Q EEIEAFPR VWSPPEDYRL

+
Macromolecule #47: uL14m

MacromoleculeName: uL14m / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 15.955632 KDa
SequenceString:
MAFCTGLRGP FAQVSRALSQ RCFSTTGSLS AIQKMTRVRV VDNSALGTTP YHRPPRCIHV YNKTGVGKVG DRILLAIRGQ KKKALIVGH RMPGPRMTPR FDSNNVVLLE DNGNPVGTRI KTPIPSSLRQ REGEFSKVLA IAQNFV

+
Macromolecule #48: uL15m

MacromoleculeName: uL15m / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 33.481723 KDa
SequenceString: MGGPVQGGGP RALDLLRALP RVSLANLKPN PGSRKPERRP RGRRRGRKCG RGHKGERQRG TRPRLGFEGG QTPFYLRIPK YGFNEGHSF RHQYQPLSLN RLQYLIDLGR VDPTQPIDLT QLVNGRGVTI QPSKRDYGVQ LVEEGADTFK AKVNIEVQMA S ELAIAAIE ...String:
MGGPVQGGGP RALDLLRALP RVSLANLKPN PGSRKPERRP RGRRRGRKCG RGHKGERQRG TRPRLGFEGG QTPFYLRIPK YGFNEGHSF RHQYQPLSLN RLQYLIDLGR VDPTQPIDLT QLVNGRGVTI QPSKRDYGVQ LVEEGADTFK AKVNIEVQMA S ELAIAAIE KNGGVVTTAF YDPRSLEILC KPVPFFLRGQ PIPKRMLPPE ALVPYYTDAK NRGYLADPAR FPEARLELAR KY GYVLPDI TKDELFKMLS TRKDPRQIFF GLAPGWVVNM ADKKILKPTD ENLLKYYSS

+
Macromolecule #49: bL17m

MacromoleculeName: bL17m / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 19.268178 KDa
SequenceString:
MRLSVAAAIS HGRVFRRLGL GPESRIHLLQ NLLTGLVRHE RIEASWARVD ELRGYAEKLI DYGKLGDTNE RAMRMADFWL TEKDLIPKL FQVLAPRFQG QNGGYTRMLQ IPNRNEQDRA KMAVIEYKGN CLPPLPLPRR DSNLTLLNQL LQGLRQDQEA S SHTAQTPA V

+
Macromolecule #50: Mitochondrial ribosomal protein L18

MacromoleculeName: Mitochondrial ribosomal protein L18 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 20.582568 KDa
SequenceString:
MALRSRFWAL LSICRNPGCR AAALSTSCKP ATNPETDPVE NEAVAPEFTN RNPRNLELLA VARKERGWGT VWPSREFWHR LRVIRTQHH IEALVEHRNG QVVVSASTRE WAIKKHLYST RNVVACESVG RVLAERCLEA GINFMVYHPT PWEAASDSIK R LQHAMTEG GVVLREPRRI YE

+
Macromolecule #51: Mitochondrial ribosomal protein L19

MacromoleculeName: Mitochondrial ribosomal protein L19 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 33.441539 KDa
SequenceString: MAASITRGSW AAVGLGQSFP PTRALLPAPA SVICRTLAGS GRQQVTGSSE PGAFQPPPKP VIVDKRRPQR PETRFLSPEF IPPRGRTNP LKFQIERKDM LERRKILHIP EFYVGSILRV TTADPYASAK TSQFLGICIQ RSGSGLGATF ILRNTIEGQG V EICFELYN ...String:
MAASITRGSW AAVGLGQSFP PTRALLPAPA SVICRTLAGS GRQQVTGSSE PGAFQPPPKP VIVDKRRPQR PETRFLSPEF IPPRGRTNP LKFQIERKDM LERRKILHIP EFYVGSILRV TTADPYASAK TSQFLGICIQ RSGSGLGATF ILRNTIEGQG V EICFELYN PRIQEIQVVK LEKRLDNSLL YLRDALPEYS TFDVNMKPIT QEFNQEVPVN QLKVKMKPKP WSKRWERPKF NI KGIRFDL CLTEEQMREA QKWSQPWLEF DMMREYDTSK IEAAIWDEIE ASKNS

+
Macromolecule #52: Mitochondrial ribosomal protein L20

MacromoleculeName: Mitochondrial ribosomal protein L20 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 17.554793 KDa
SequenceString:
MVFLTVPLWL RSRVTDRYWR VQEVLKHARH FRGRKNRCYR LAVRAVTRAF VKCTRARRLK KRSLRTLWIN RITAASQEHG LKYPAFIIN LIKCQVELNR KVLADLAIYE PKTFKSLAAL AKRRREEGFA AALGDGKEPD GIFSRVVQHR

+
Macromolecule #53: Mitochondrial ribosomal protein L21

MacromoleculeName: Mitochondrial ribosomal protein L21 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 23.154861 KDa
SequenceString: MAAAVAASVL QGTFGRLVSA YSRSVLRTSR PGTAPPWSAP RRLSSQHTSS LRGCVPKTSL GSPPWPDIVL PDPVEETRHH AEVVGQVNE LIAAGRYGRL FAVVHFAGHQ WKVTAEDLIL IENELDVACG ERIRLEKVLL VGADNFTLLG KPLLGKELVR V EATVIEKT ...String:
MAAAVAASVL QGTFGRLVSA YSRSVLRTSR PGTAPPWSAP RRLSSQHTSS LRGCVPKTSL GSPPWPDIVL PDPVEETRHH AEVVGQVNE LIAAGRYGRL FAVVHFAGHQ WKVTAEDLIL IENELDVACG ERIRLEKVLL VGADNFTLLG KPLLGKELVR V EATVIEKT ESWPKINMKF QKRKNYQRKR ITVNPQTVLR INSIEIAPCL C

+
Macromolecule #54: uL22m

MacromoleculeName: uL22m / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 24.313357 KDa
SequenceString: MAAAVLERLG GLWMQNLRGK LALRLLSSHS ILPQSHIHTS ASVEISRKWE KKNKIVYPPQ LPGEPRRPAE IYHCRRQIKY SKDKMWYLA KLIRGMSIDQ ALAQLEFSDK KGAQIIKEVL LEAQDMAVRD HNVEFRSNLY VAESTSGRGQ YLKRIRYHGR G RFGIMEKV ...String:
MAAAVLERLG GLWMQNLRGK LALRLLSSHS ILPQSHIHTS ASVEISRKWE KKNKIVYPPQ LPGEPRRPAE IYHCRRQIKY SKDKMWYLA KLIRGMSIDQ ALAQLEFSDK KGAQIIKEVL LEAQDMAVRD HNVEFRSNLY VAESTSGRGQ YLKRIRYHGR G RFGIMEKV FCHYFVKLVE GPPPPREAPK TAVAHAREYI QELRNRTITH AL

+
Macromolecule #55: uL23m

MacromoleculeName: uL23m / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 17.643205 KDa
SequenceString:
MARNVLYPLY QLGNPQLRVF RTNFFIQLVR PGTAQPEDTV QFRIPMEMTR VDLRNYLERI YNVPVAAVRT RVQYGSNRRR DHRNIRIKK PDYKVAYVQL ALGQTFTFPD LFPERKGASV DVDVRDQVLE DQRQKHSPDP RRGGVPGWFG L

+
Macromolecule #29: 16S rRNA

MacromoleculeName: 16S rRNA / type: rna / ID: 29 / Number of copies: 1
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 504.852031 KDa
SequenceString: ACCAAAGCUA GCUCAACAUA CUAAACAAAU ACAAAAAUAC ACCAAAAUAA AAUAAAACAU UCACCUAACA UUAAAGUAUA GGAGAUAGA AAUUUUUAUC CUGACGCUAU AGAGAUAGUA CCGUAAGGGA AAGAUGAAAG AAUAAAAUAA AAGUAAAAAA A AGCAAAGA ...String:
ACCAAAGCUA GCUCAACAUA CUAAACAAAU ACAAAAAUAC ACCAAAAUAA AAUAAAACAU UCACCUAACA UUAAAGUAUA GGAGAUAGA AAUUUUUAUC CUGACGCUAU AGAGAUAGUA CCGUAAGGGA AAGAUGAAAG AAUAAAAUAA AAGUAAAAAA A AGCAAAGA UUACCCCUUC UACCUUUUGC AUAAUGGUUU AACCAGAAAA AAUCUAACAA AGAGAACUUU AGCUAGAUAC CC CGAAACC AGACGAGCUA CCCAUGAGCA GUUUAAAAGA ACCAACUCAU CUAUGUGGCA AAAUAGUGAG AAGACUUGUA GGU AGAGGU GAAAAGCCUA ACGAGCCUGG UGAUAGCUGG UUGUCCGAGA AAGAAUUUUA GUUCAACCUU AAAAAUACCC CAAA AACCC UAAAUUCCAA UGUAUUUUUA AGAGAUAGUC UAAAAAGGUA CAGCUUUUUA GAAACGGAUA CAACCUUGAC UAGAG AGUA AAAUCUUAAU ACUACCAUAG UAGGCCUAAA AGCAGCCAUC AAUUGAGAAA GCGUUAAAGC UCAACAAAUU CACCAA CAU AAUCCCAAAA ACUAAUAACA AACUCCUAGC CCAAUACCGG ACUAAUCUAU UGAAACAUAG AAGCAAUAAU GUUAAUA UG AGUAACAAGA AGCCUUUCUC CUCGCACACG CUUACAUCAG UAACUAAUAA UAUACUGAUA AUUAACAACC AAUAAACC A AAACAACACU AAAACGUUUA UUAAUUACAU UGUUAACCCA ACACAGGAGU GCACCAAGGA AAGAUUAAAA GAAGUAAAA GGAACUCGGC AAACACAAAC CCCGCCUGUU UACCAAAAAC AUCACCUCUA GCAUUACUAG UAUUAGAGGC AAUGCCUGCC CAGUGACAC CAGUUUAACG GCCGCGGUAU UCUGACCGUG CAAAGGUAGC AUAAUCACUU GUUCUCCAAA UAAGGACUUG U AUGAAUGG CCACACGAGG GUUUUACUGU CUCUUACUUC CAAUCAGUGA AAUUAACCUU CCCGUGAAGA GGCGGGAAUA AA AAAAUAA GACGAGAAGA CCCUAUGGAG CUUUAAUUAA CUAUUCCAAA AGUUAAACAA CUCAACCACA AAGGGAUAAA ACA UAACUU AACAUGGACU AGCAAUUUCG GUUGGGGUGA CCUCGGAGUA CAAAAAACCC UCCGAGUGAU UUUAAUCUAG ACAA ACCAG UCAAAAUAAC CAUAACAUCA CUUAUUGAUC CAAAAUUUUG AUCAACGGAA CAAGUUACCC UAGGGAUAAC AGCGC AAUC CUGUUCUAGA GUUCCUAUCG ACAAUAGGGU UUACGACCUC GAUGUUGGAU CAGGACACCC AAAUGGUGCA ACCGCU AUU AAAGGUUCGU UUGUUCAACG AUUAAAGUCC UACGUGAUCU GAGUUCAGAC CGGAGCAAUC CAGGUCGGUU UCUAUCU AU UAUAAAUUUC UCCCAGUACG AAAGGACAAG AGAAAUGGGA CCAACCUCAC AAACGCGUCU CAGAGAUAAU UAAUGAUU U AAUCUUAACC UAAUUAACUC AUAAUAAAUC CAGCCCUAGA ACAGGGCACA

+
Macromolecule #36: CP tRNAPhe

MacromoleculeName: CP tRNAPhe / type: rna / ID: 36 / Number of copies: 1
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 23.402018 KDa
SequenceString:
GUUAAUGUAG CUUAAAUUAU CAAAGCAAGG CACUGAAAAU GCCUAGAUGA GCCUCACAGC UCCAUAAACA CCA

+
Macromolecule #56: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 56 / Number of copies: 221 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #57: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 57 / Number of copies: 3 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #58: GUANOSINE-5'-MONOPHOSPHATE

MacromoleculeName: GUANOSINE-5'-MONOPHOSPHATE / type: ligand / ID: 58 / Number of copies: 2 / Formula: 5GP
Molecular weightTheoretical: 363.221 Da
Chemical component information

ChemComp-5GP:
GUANOSINE-5'-MONOPHOSPHATE / Guanosine monophosphate

+
Macromolecule #59: SPERMINE

MacromoleculeName: SPERMINE / type: ligand / ID: 59 / Number of copies: 2 / Formula: SPM
Molecular weightTheoretical: 202.34 Da
Chemical component information

ChemComp-SPM:
SPERMINE / Spermine

+
Macromolecule #60: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER

MacromoleculeName: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / type: ligand / ID: 60 / Number of copies: 1 / Formula: GNP
Molecular weightTheoretical: 522.196 Da
Chemical component information

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM / 5'-Guanylyl imidodiphosphate

+
Macromolecule #61: water

MacromoleculeName: water / type: ligand / ID: 61 / Number of copies: 2 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

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Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.6
VitrificationCryogen name: ETHANE-PROPANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Average electron dose: 40.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 224731

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