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- EMDB-11727: Cryo-EM structure of the mycoplasma MIB-MIP proteins in complex w... -

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Basic information

Entry
Database: EMDB / ID: EMD-11727
TitleCryo-EM structure of the mycoplasma MIB-MIP proteins in complex with a goat Fab
Map data
Sample
  • Complex: Mycoplasma MIB-MIP proteins in complex with a goat Fab
    • Protein or peptide: Putative immunoglobulin-blocking virulence protein
Function / homologyMycoplasma virulence, signal domain / Putative immunoglobulin-blocking virulence protein / Mycoplasma virulence signal region (Myco_arth_vir_N) / IgG-blocking virulence domain / Putative immunoglobulin-blocking virulence protein
Function and homology information
Biological speciesMycoplasma mycoides subsp. capri str. GM12 (bacteria) / Mycoplasma mycoides subsp. capri (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.8 Å
AuthorsNottelet P / Bataille L / Gourgues G / Anger R / Lartigue C / Sirand-Pugnet P / Marza E / Fronzes R / Arfi Y
Funding support France, 1 items
OrganizationGrant numberCountry
French National Research Agency France
CitationJournal: Sci Adv / Year: 2021
Title: The mycoplasma surface proteins MIB and MIP promote the dissociation of the antibody-antigen interaction.
Authors: Pierre Nottelet / Laure Bataille / Geraldine Gourgues / Robin Anger / Carole Lartigue / Pascal Sirand-Pugnet / Esther Marza / Remi Fronzes / Yonathan Arfi /
Abstract: Mycoplasma immunoglobulin binding (MIB) and mycoplasma immunoglobulin protease (MIP) are surface proteins found in the majority of mycoplasma species, acting sequentially to capture antibodies and ...Mycoplasma immunoglobulin binding (MIB) and mycoplasma immunoglobulin protease (MIP) are surface proteins found in the majority of mycoplasma species, acting sequentially to capture antibodies and cleave off their V domains. Cryo-electron microscopy structures show how MIB and MIP bind to a Fab fragment in a "hug of death" mechanism. As a result, the orientation of the V and V domains is twisted out of alignment, disrupting the antigen binding site. We also show that MIB-MIP has the ability to promote the dissociation of the antibody-antigen complex. This system is functional in cells and protects mycoplasmas from antibody-mediated agglutination. These results highlight the key role of the MIB-MIP system in immunity evasion by mycoplasmas through an unprecedented mechanism, and open exciting perspectives to use these proteins as potential tools in the antibody field.
History
DepositionSep 15, 2020-
Header (metadata) releaseApr 7, 2021-
Map releaseApr 7, 2021-
UpdateApr 7, 2021-
Current statusApr 7, 2021Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 3
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 3
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7adj
  • Surface level: 4
  • Imaged by UCSF Chimera
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-7adj
  • Imaged by Jmol
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Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_11727.png

Downloads & links

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Map

FileDownload / File: emd_11727.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 0.83 Å
Density
Contour LevelBy AUTHOR: 3.0 / Movie #1: 3
Minimum - Maximum-24.763588 - 29.809916
Average (Standard dev.)3.5810315e-12 (±1.0)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 212.48 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z0.830.830.83
M x/y/z256256256
origin x/y/z0.0000.0000.000
length x/y/z212.480212.480212.480
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ256256256
MAP C/R/S321
start NC/NR/NS000
NC/NR/NS256256256
D min/max/mean-24.76429.8100.000

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Supplemental data

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Half map: #2

Fileemd_11727_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_11727_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Mycoplasma MIB-MIP proteins in complex with a goat Fab

EntireName: Mycoplasma MIB-MIP proteins in complex with a goat Fab
Components
  • Complex: Mycoplasma MIB-MIP proteins in complex with a goat Fab
    • Protein or peptide: Putative immunoglobulin-blocking virulence protein

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Supramolecule #1: Mycoplasma MIB-MIP proteins in complex with a goat Fab

SupramoleculeName: Mycoplasma MIB-MIP proteins in complex with a goat Fab
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Mycoplasma mycoides subsp. capri str. GM12 (bacteria)
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria) / Recombinant strain: Rosetta

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Macromolecule #1: Putative immunoglobulin-blocking virulence protein

MacromoleculeName: Putative immunoglobulin-blocking virulence protein / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma mycoides subsp. capri (bacteria)
Molecular weightTheoretical: 82.922141 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: MGSSHHHHHH SSGLVPRGSH ISFDTSSNGI TDAELAPINN AINDAIVSNR DNKLKPSEEK IIKETEKKIE EKIIIPPAKK EEKIEAAKP IPKPVVRKPE TKITSPKITR RKQTITIAGI EVEAEIEGPP GFVTHQRDKD RKISNPTKPY QNHTVNKILS V KVTDKLKE ...String:
MGSSHHHHHH SSGLVPRGSH ISFDTSSNGI TDAELAPINN AINDAIVSNR DNKLKPSEEK IIKETEKKIE EKIIIPPAKK EEKIEAAKP IPKPVVRKPE TKITSPKITR RKQTITIAGI EVEAEIEGPP GFVTHQRDKD RKISNPTKPY QNHTVNKILS V KVTDKLKE QVAKDALSGG NGYDEGVGLF NNSIFNVFKE EFNSGKELND ILSSLESVAR QNSGAFQNTL ERYKKMLDSN NV INFLKSE AQKEYPKLKS KFQTKNQEYI WLIANLDQSK FTKIASTSEK YLEKGLTISP RSAFINEAGE IDSNGWGPPD EYN TVTSRL RRDNSEYRVF DYDEYYSRSS DRIANGTYPG WVKEDVSEPY SKKYNFKASD GIRFSKLERI NPNPAKGKLN SGLV LDLDV SNDEAYRRSK ELIEKLQKDG EQITSYRIKN MGEKNSDQAF KDILGALPKD IQQLELFFSD KATNTASLIA LENKN IKEL SLYTSGNSLK KAWSYNPLAL RNTTWINTID YNVSAEYSSH DKITTRITFN TLAFDQEDFS NGSYERINDG LRMVYY ARN NEPFFQGGHG PGLEPDKKLG QNSYPTGLDF SRVTGIKSLK GLRFDDDLDT SNEPRKITEL TLYNNESYFE ISSDELN EA NLQHLSTGEG NPEKPKIHFS NGNNTTSIRI SGKTLLSDEG RRNLDKYFEY NESLRNSGKQ IQIPNGSDEL KKQLEGWG Y KVSTASDRSF T

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
GridModel: C-flat / Material: COPPER / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 4 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 1.45 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: Gctf
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 255911

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