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- EMDB-10263: Structure of Coxsackievirus A10 complexed with its receptor KREMEN1 -

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Basic information

Entry
Database: EMDB / ID: EMD-10263
TitleStructure of Coxsackievirus A10 complexed with its receptor KREMEN1
Map data
Sample
  • Complex: Coxsackievirus A10
    • Complex: Coxsackievirus A10
      • Protein or peptide: Capsid protein VP1
      • Protein or peptide: Coxsackievirus VP2
      • Protein or peptide: Capsid protein VP3
      • Protein or peptide: Coxsackievirus VP4
    • Complex: KREMEN1
      • Protein or peptide: Kremen protein 1
  • Ligand: SPHINGOSINE
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
Function / homology
Function and homology information


Signaling by LRP5 mutants / Negative regulation of TCF-dependent signaling by WNT ligand antagonists / negative regulation of axon regeneration / cell communication / negative regulation of ossification / regulation of canonical Wnt signaling pathway / limb development / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MDA-5 activity / picornain 2A / symbiont-mediated suppression of host mRNA export from nucleus ...Signaling by LRP5 mutants / Negative regulation of TCF-dependent signaling by WNT ligand antagonists / negative regulation of axon regeneration / cell communication / negative regulation of ossification / regulation of canonical Wnt signaling pathway / limb development / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MDA-5 activity / picornain 2A / symbiont-mediated suppression of host mRNA export from nucleus / symbiont genome entry into host cell via pore formation in plasma membrane / picornain 3C / T=pseudo3 icosahedral viral capsid / TCF dependent signaling in response to WNT / host cell cytoplasmic vesicle membrane / negative regulation of canonical Wnt signaling pathway / cytoplasmic vesicle membrane / endocytosis involved in viral entry into host cell / Wnt signaling pathway / : / nucleoside-triphosphate phosphatase / protein complex oligomerization / monoatomic ion channel activity / RNA helicase activity / induction by virus of host autophagy / RNA-directed RNA polymerase / symbiont-mediated suppression of host gene expression / viral RNA genome replication / cysteine-type endopeptidase activity / RNA-dependent RNA polymerase activity / neuronal cell body / DNA-templated transcription / apoptotic process / virion attachment to host cell / structural molecule activity / ATP hydrolysis activity / proteolysis / RNA binding / ATP binding / membrane / metal ion binding / plasma membrane
Similarity search - Function
Kremen / Carbohydrate-binding WSC / WSC domain / WSC domain profile. / present in yeast cell wall integrity and stress response component proteins / CUB domain / Domain first found in C1r, C1s, uEGF, and bone morphogenetic protein. / CUB domain / CUB domain profile. / Spermadhesin, CUB domain superfamily ...Kremen / Carbohydrate-binding WSC / WSC domain / WSC domain profile. / present in yeast cell wall integrity and stress response component proteins / CUB domain / Domain first found in C1r, C1s, uEGF, and bone morphogenetic protein. / CUB domain / CUB domain profile. / Spermadhesin, CUB domain superfamily / Kringle domain / Kringle / Kringle, conserved site / Kringle superfamily / Kringle domain signature. / Kringle domain profile. / Kringle domain / Kringle-like fold / Poliovirus 3A protein-like / Poliovirus 3A protein like / Picornavirus 2B protein / Poliovirus core protein 3a, soluble domain / Picornavirus 2B protein / Peptidase C3, picornavirus core protein 2A / Picornavirus core protein 2A / Picornavirus coat protein VP4 / Picornavirus coat protein (VP4) / Picornavirales 3C/3C-like protease domain / Picornavirales 3C/3C-like protease domain profile. / Peptidase C3A/C3B, picornaviral / 3C cysteine protease (picornain 3C) / Picornavirus capsid / picornavirus capsid protein / Helicase, superfamily 3, single-stranded RNA virus / Superfamily 3 helicase of positive ssRNA viruses domain profile. / Helicase, superfamily 3, single-stranded DNA/RNA virus / RNA helicase / Picornavirus/Calicivirus coat protein / Viral coat protein subunit / RNA-directed RNA polymerase, C-terminal domain / Viral RNA-dependent RNA polymerase / Reverse transcriptase/Diguanylate cyclase domain / RNA-directed RNA polymerase, catalytic domain / RdRp of positive ssRNA viruses catalytic domain profile. / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / Peptidase S1, PA clan, chymotrypsin-like fold / Peptidase S1, PA clan / DNA/RNA polymerase superfamily / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Genome polyprotein / Kremen protein 1
Similarity search - Component
Biological speciesCoxsackievirus A10 / Homo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.9 Å
AuthorsZhao Y / Zhou D / Ni T / Karia D / Kotecha A / Wang X / Rao Z / Jones EY / Fry EE / Ren J / Stuart DI
Funding support United Kingdom, 3 items
OrganizationGrant numberCountry
Medical Research Council (United Kingdom)MR/N00065X/1 United Kingdom
Cancer Research UKC375/A17721 United Kingdom
Wellcome Trust101122/Z/13/Z United Kingdom
CitationJournal: Nat Commun / Year: 2020
Title: Hand-foot-and-mouth disease virus receptor KREMEN1 binds the canyon of Coxsackie Virus A10.
Authors: Yuguang Zhao / Daming Zhou / Tao Ni / Dimple Karia / Abhay Kotecha / Xiangxi Wang / Zihe Rao / E Yvonne Jones / Elizabeth E Fry / Jingshan Ren / David I Stuart /
Abstract: Coxsackievirus A10 (CV-A10) is responsible for an escalating number of severe infections in children, but no prophylactics or therapeutics are currently available. KREMEN1 (KRM1) is the entry ...Coxsackievirus A10 (CV-A10) is responsible for an escalating number of severe infections in children, but no prophylactics or therapeutics are currently available. KREMEN1 (KRM1) is the entry receptor for the largest receptor-group of hand-foot-and-mouth disease causing viruses, which includes CV-A10. We report here structures of CV-A10 mature virus alone and in complex with KRM1 as well as of the CV-A10 A-particle. The receptor spans the viral canyon with a large footprint on the virus surface. The footprint has some overlap with that seen for the neonatal Fc receptor complexed with enterovirus E6 but is larger and distinct from that of another enterovirus receptor SCARB2. Reduced occupancy of a particle-stabilising pocket factor in the complexed virus and the presence of both unbound and expanded virus particles suggests receptor binding initiates a cascade of conformational changes that produces expanded particles primed for viral uncoating.
History
DepositionAug 27, 2019-
Header (metadata) releaseSep 4, 2019-
Map releaseJan 15, 2020-
UpdateJul 29, 2020-
Current statusJul 29, 2020Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.01
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  • Surface view colored by radius
  • Surface level: 0.01
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  • Surface view with fitted model
  • Atomic models: PDB-6snw
  • Surface level: 0.01
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-6snw
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_10263.png

Downloads & links

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Map

FileDownload / File: emd_10263.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.35 Å
Density
Contour LevelBy AUTHOR: 0.01 / Movie #1: 0.01
Minimum - Maximum-0.05129708 - 0.097188205
Average (Standard dev.)0.0006619525 (±0.0049746973)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 540.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.351.351.35
M x/y/z400400400
origin x/y/z0.0000.0000.000
length x/y/z540.000540.000540.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS400400400
D min/max/mean-0.0510.0970.001

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Supplemental data

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Sample components

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Entire : Coxsackievirus A10

EntireName: Coxsackievirus A10
Components
  • Complex: Coxsackievirus A10
    • Complex: Coxsackievirus A10
      • Protein or peptide: Capsid protein VP1
      • Protein or peptide: Coxsackievirus VP2
      • Protein or peptide: Capsid protein VP3
      • Protein or peptide: Coxsackievirus VP4
    • Complex: KREMEN1
      • Protein or peptide: Kremen protein 1
  • Ligand: SPHINGOSINE
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose

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Supramolecule #1: Coxsackievirus A10

SupramoleculeName: Coxsackievirus A10 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#5

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Supramolecule #2: Coxsackievirus A10

SupramoleculeName: Coxsackievirus A10 / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#4
Source (natural)Organism: Coxsackievirus A10 / Strain: Kowalik

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Supramolecule #3: KREMEN1

SupramoleculeName: KREMEN1 / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #5
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Homo sapiens (human) / Recombinant cell: HEK293S

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Macromolecule #1: Capsid protein VP1

MacromoleculeName: Capsid protein VP1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: picornain 2A
Source (natural)Organism: Coxsackievirus A10
Molecular weightTheoretical: 33.191422 KDa
SequenceString: GDPVEDIIHD ALSSTVRRAI TSGQDVNTAA GTAPSSHRLE TGRVPALQAA ETGATSNATD ENMIETRCVM NRNGVLEATI SHFFSRSGL VGVVNLTDGG TDTTGYAVWD IDIMGFVQLR RKCEMFTYMR FNAEFTFVTT TENGEARPFM LQYMYVPPGA P KPTGRDAF ...String:
GDPVEDIIHD ALSSTVRRAI TSGQDVNTAA GTAPSSHRLE TGRVPALQAA ETGATSNATD ENMIETRCVM NRNGVLEATI SHFFSRSGL VGVVNLTDGG TDTTGYAVWD IDIMGFVQLR RKCEMFTYMR FNAEFTFVTT TENGEARPFM LQYMYVPPGA P KPTGRDAF QWQTATNPSV FVKLTDPPAQ VSVPFMSPAS AYQWFYDGYP TFGQHPETSN TTYGQCPNNM MGTFAVRVVS RV ASQLKLQ TRVYMKLKHV RAWIPRPIRS QPYLLKNFPN YDSSKITYSA RDRASIKQAN M

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Macromolecule #2: Coxsackievirus VP2

MacromoleculeName: Coxsackievirus VP2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: picornain 2A
Source (natural)Organism: Coxsackievirus A10
Molecular weightTheoretical: 27.72998 KDa
SequenceString: SPSVEACGYS DRVAQLTVGN SSITTQEAAN IVLAYGEWPE YCPDTDATAV DKPTRPDVSV NRFYTLDSKM WQENSTGWYW KFPDVLNKT GVFGQNAQFH YLYRSGFCLH VQCNASKFHQ GALLVAVIPE FVLAGRGSNT KPNEAPHPGF NTTFPGTAGA S FNDPYVLD ...String:
SPSVEACGYS DRVAQLTVGN SSITTQEAAN IVLAYGEWPE YCPDTDATAV DKPTRPDVSV NRFYTLDSKM WQENSTGWYW KFPDVLNKT GVFGQNAQFH YLYRSGFCLH VQCNASKFHQ GALLVAVIPE FVLAGRGSNT KPNEAPHPGF NTTFPGTAGA S FNDPYVLD SGVPLSQSLI YPHQWINLRT NNCATIIVPY INAVPFDSAI NHSNFGLIVV PVSPLKYSSG ATTAIPITVT IA PLNSEFG GLRQAVSQ

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Macromolecule #3: Capsid protein VP3

MacromoleculeName: Capsid protein VP3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: picornain 2A
Source (natural)Organism: Coxsackievirus A10
Molecular weightTheoretical: 26.219672 KDa
SequenceString: GLPTELRPGT NQFLTTEDDT AAPILPGFSP TPSIHIPGEV RSLLELCRVE TILEVNNTTD ATGLNRLLIP VSAQNKADEL CAAFMVDPG RIGPWQSTLV GQICRYYTQW SGSLKVTFMF TGSFMATGKM LIAYSPPGSA QPANRETAML GTHVIWDFGL Q SSVSLVIP ...String:
GLPTELRPGT NQFLTTEDDT AAPILPGFSP TPSIHIPGEV RSLLELCRVE TILEVNNTTD ATGLNRLLIP VSAQNKADEL CAAFMVDPG RIGPWQSTLV GQICRYYTQW SGSLKVTFMF TGSFMATGKM LIAYSPPGSA QPANRETAML GTHVIWDFGL Q SSVSLVIP WISNTHFRTA KTGGNYDYYT AGVVTLWYQT NYVVPPETPG EAYIIAMGAA QDNFTLKICK DTDEVTQQAV LQ

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Macromolecule #4: Coxsackievirus VP4

MacromoleculeName: Coxsackievirus VP4 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: picornain 2A
Source (natural)Organism: Coxsackievirus A10
Molecular weightTheoretical: 7.464065 KDa
SequenceString:
MGAQVSSQRS GSHETGNVAT GGSTINFTNI NYYKDSYAAS ASRQDFTQDP KKFTQPVLDS IRELSAPLN

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Macromolecule #5: Kremen protein 1

MacromoleculeName: Kremen protein 1 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 41.786293 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: ETGAPSPGLG PGPECFTANG ADYRGTQNWT ALQGGKPCLF WNETFQHPYN TLKYPNGEGG LGEHNYCRNP DGDVSPWCYV AEHEDGVYW KYCEIPACQM PGNLGCYKDH GNPPPLTGTS KTSNKLTIQT CISFCRSQRF KFAGMESGYA CFCGNNPDYW K YGEAASTE ...String:
ETGAPSPGLG PGPECFTANG ADYRGTQNWT ALQGGKPCLF WNETFQHPYN TLKYPNGEGG LGEHNYCRNP DGDVSPWCYV AEHEDGVYW KYCEIPACQM PGNLGCYKDH GNPPPLTGTS KTSNKLTIQT CISFCRSQRF KFAGMESGYA CFCGNNPDYW K YGEAASTE CNSVCFGDHT QPCGGDGRII LFDTLVGACG GNYSAMSSVV YSPDFPDTYA TGRVCYWTIR VPGASHIHFS FP LFDIRDS ADMVELLDGY THRVLARFHG RSRPPLSFNV SLDFVILYFF SDRINQAQGF AVLYQAVKEE GSENLYFQGG SLP QERPAV NQTVAEVITE QANLSVSAAR SSKVLYVITT SPSHPPQTVP GTHHHHHHHH HH

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Macromolecule #6: SPHINGOSINE

MacromoleculeName: SPHINGOSINE / type: ligand / ID: 6 / Number of copies: 1 / Formula: SPH
Molecular weightTheoretical: 299.492 Da
Chemical component information

ChemComp-SPH:
SPHINGOSINE / Sphingosine

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Macromolecule #7: 2-acetamido-2-deoxy-beta-D-glucopyranose

MacromoleculeName: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 7 / Number of copies: 2 / Formula: NAG
Molecular weightTheoretical: 221.208 Da
Chemical component information

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI POLARA 300
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Number grids imaged: 1 / Average electron dose: 35.0 e/Å2
Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company

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Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

5c4w

Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 1597

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-6snw:
Structure of Coxsackievirus A10 complexed with its receptor KREMEN1

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