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Yorodumi- EMDB-10136: Double-stranded helical ESCRT-III filament formed from Snf7/Vps24... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-10136 | |||||||||||||||
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Title | Double-stranded helical ESCRT-III filament formed from Snf7/Vps24/Vps2 on a helical membrane bicelle | |||||||||||||||
Map data | Masked double-stranded helical ESCRT-III filament formed from Snf7/Vps24/Vps2 on a helical bicelle | |||||||||||||||
Sample |
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Biological species | Saccharomyces cerevisiae (brewer's yeast) | |||||||||||||||
Method | helical reconstruction / cryo EM / Resolution: 14.1 Å | |||||||||||||||
Authors | Frost A / Johnson I / Talledge N | |||||||||||||||
Funding support | United States, 4 items
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Citation | Journal: Nat Commun / Year: 2020 Title: Anisotropic ESCRT-III architecture governs helical membrane tube formation. Authors: Joachim Moser von Filseck / Luca Barberi / Nathaniel Talledge / Isabel E Johnson / Adam Frost / Martin Lenz / Aurélien Roux / Abstract: ESCRT-III proteins assemble into ubiquitous membrane-remodeling polymers during many cellular processes. Here we describe the structure of helical membrane tubes that are scaffolded by bundled ESCRT- ...ESCRT-III proteins assemble into ubiquitous membrane-remodeling polymers during many cellular processes. Here we describe the structure of helical membrane tubes that are scaffolded by bundled ESCRT-III filaments. Cryo-ET reveals how the shape of the helical membrane tube arises from the assembly of two distinct bundles of helical filaments that have the same helical path but bind the membrane with different interfaces. Higher-resolution cryo-EM of filaments bound to helical bicelles confirms that ESCRT-III filaments can interact with the membrane through a previously undescribed interface. Mathematical modeling demonstrates that the interface described above is key to the mechanical stability of helical membrane tubes and helps infer the rigidity of the described protein filaments. Altogether, our results suggest that the interactions between ESCRT-III filaments and the membrane could proceed through multiple interfaces, to provide assembly on membranes with various shapes, or adapt the orientation of the filaments towards the membrane during membrane remodeling. | |||||||||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_10136.map.gz | 3.7 MB | EMDB map data format | |
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Header (meta data) | emd-10136-v30.xml emd-10136.xml | 15.9 KB 15.9 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_10136_fsc.xml | 8 KB | Display | FSC data file |
Images | emd_10136.png | 40.8 KB | ||
Others | emd_10136_additional.map.gz emd_10136_additional_1.map.gz emd_10136_half_map_1.map.gz emd_10136_half_map_2.map.gz | 36.1 MB 36.1 MB 31.1 MB 31.1 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-10136 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-10136 | HTTPS FTP |
-Related structure data
Related structure data | C: citing same article (ref.) |
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Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_10136.map.gz / Format: CCP4 / Size: 40.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Masked double-stranded helical ESCRT-III filament formed from Snf7/Vps24/Vps2 on a helical bicelle | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 2.76 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Additional map: Unmasked double-stranded helical ESCRT-III filament formed from Snf7/Vps24/Vps2...
File | emd_10136_additional.map | ||||||||||||
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Annotation | Unmasked double-stranded helical ESCRT-III filament formed from Snf7/Vps24/Vps2 on a helical bicelle | ||||||||||||
Projections & Slices |
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Density Histograms |
-Additional map: Unmasked double-stranded helical ESCRT-III filament formed from Snf7/Vps24/Vps2...
File | emd_10136_additional_1.map | ||||||||||||
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Annotation | Unmasked double-stranded helical ESCRT-III filament formed from Snf7/Vps24/Vps2 on a helical bicelle | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_10136_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_10136_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Double-stranded helical ESCRT-III filament formed from Snf7/Vps24...
Entire | Name: Double-stranded helical ESCRT-III filament formed from Snf7/Vps24/Vps2 on a helical bicelle |
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Components |
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-Supramolecule #1: Double-stranded helical ESCRT-III filament formed from Snf7/Vps24...
Supramolecule | Name: Double-stranded helical ESCRT-III filament formed from Snf7/Vps24/Vps2 on a helical bicelle type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Saccharomyces cerevisiae (brewer's yeast) |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Molecular weight | Experimental: 30 KDa |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | helical reconstruction |
Aggregation state | filament |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy |
Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 67.2 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |