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- EMDB-0909: core region reconstruction. -

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ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-0909
Titlecore region reconstruction.
Map datacore region reconstruction
Sample
  • Complex: Cytoplasmic Ring Subunit of Xenopus laevis NPC
    • Protein or peptide: x 18 types
Function / homology
Function and homology information


macromolecule localization / nitrogen compound transport / GATOR2 complex / nephron development / organic substance transport / macromolecule metabolic process / nuclear pore organization / nuclear pore outer ring / attachment of mitotic spindle microtubules to kinetochore / structural constituent of nuclear pore ...macromolecule localization / nitrogen compound transport / GATOR2 complex / nephron development / organic substance transport / macromolecule metabolic process / nuclear pore organization / nuclear pore outer ring / attachment of mitotic spindle microtubules to kinetochore / structural constituent of nuclear pore / nucleocytoplasmic transport / mitotic metaphase chromosome alignment / cellular response to nutrient levels / intracellular transport / mRNA transport / nuclear pore / positive regulation of TORC1 signaling / kinetochore / protein transport / nuclear membrane / lysosomal membrane / cell division / structural molecule activity / metal ion binding / nucleus / cytosol
Similarity search - Function
Nuclear pore complex protein NUP98-NUP96 / Nucleoporin Nup37 / Nucleoporin Nup85-like / Nucleoporin Nup120/160 / Nup85 Nucleoporin / Nuclear pore protein 84/107 / Nuclear pore protein 84 / 107 / Nuclear pore complex protein Nup133-like / Nup358/RanBP2 E3 ligase domain / Nup358/RanBP2 E3 ligase domain ...Nuclear pore complex protein NUP98-NUP96 / Nucleoporin Nup37 / Nucleoporin Nup85-like / Nucleoporin Nup120/160 / Nup85 Nucleoporin / Nuclear pore protein 84/107 / Nuclear pore protein 84 / 107 / Nuclear pore complex protein Nup133-like / Nup358/RanBP2 E3 ligase domain / Nup358/RanBP2 E3 ligase domain / Nucleoporin Nup186/Nup192/Nup205 / Nuclear pore complex scaffold, nucleoporins 186/192/205 / Nucleoporin, Nup133/Nup155-like, C-terminal / Nucleoporin FG repeat / Nucleoporin FG repeat region / Non-repetitive/WGA-negative nucleoporin C-terminal / Nucleoporin, Nup133/Nup155-like, N-terminal / Nup133 N terminal like / Sec13/Seh1 family / Nuclear pore complex protein NUP96, C-terminal domain / Nuclear protein 96 / Nuclear pore complex protein Nup98-Nup96-like, autopeptidase S59 domain / Nuclear pore complex protein Nup98-Nup96-like, autopeptidase S59 domain superfamily / Nucleoporin autopeptidase / NUP C-terminal domain profile. / Nucleoporin peptidase S59-like / Ran binding protein RanBP1-like / Ran binding domain / RanBP1 domain / Ran binding domain type 1 profile. / Ran-binding domain / Zinc finger domain / Zn-finger in Ran binding protein and others / Zinc finger RanBP2 type profile. / Zinc finger RanBP2-type signature. / Zinc finger, RanBP2-type superfamily / Zinc finger, RanBP2-type / TPR repeat profile. / Tetratricopeptide repeats / Tetratricopeptide repeat / Tetratricopeptide-like helical domain superfamily / PH-like domain superfamily / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
Similarity search - Domain/homology
Uncharacterized protein / Nuclear pore complex protein Nup133 / Nuclear pore complex protein Nup98-Nup96 / RANBP2-like and GRIP domain-containing protein 3 isoform X2 / Nuclear pore complex protein / MGC154553 protein / Nucleoporin SEH1-A / MGC83295 protein / MGC83926 protein / Nuclear pore complex protein Nup85 / Protein SEC13 homolog
Similarity search - Component
Biological speciesXenopus laevis (African clawed frog) / African clawed frog (African clawed frog)
Methodsingle particle reconstruction / cryo EM / Resolution: 5.5 Å
AuthorsShi Y / Huang G / Yan C / Zhang Y
Funding support China, 2 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)31430020 China
National Natural Science Foundation of China (NSFC)31621092 China
CitationJournal: Cell Res / Year: 2020
Title: Structure of the cytoplasmic ring of the Xenopus laevis nuclear pore complex by cryo-electron microscopy single particle analysis.
Authors: Gaoxingyu Huang / Yanqing Zhang / Xuechen Zhu / Chao Zeng / Qifan Wang / Qiang Zhou / Qinghua Tao / Minhao Liu / Jianlin Lei / Chuangye Yan / Yigong Shi /
Abstract: The nuclear pore complex (NPC) exhibits structural plasticity and has only been characterized at local resolutions of up to 15 Å for the cytoplasmic ring (CR). Here we present a single-particle ...The nuclear pore complex (NPC) exhibits structural plasticity and has only been characterized at local resolutions of up to 15 Å for the cytoplasmic ring (CR). Here we present a single-particle cryo-electron microscopy (cryo-EM) structure of the CR from Xenopus laevis NPC at average resolutions of 5.5-7.9 Å, with local resolutions reaching 4.5 Å. Improved resolutions allow identification and placement of secondary structural elements in the majority of the CR components. The two Y complexes in each CR subunit interact with each other and associate with those from flanking subunits, forming a circular scaffold. Within each CR subunit, the Nup358-containing region wraps around the stems of both Y complexes, likely stabilizing the scaffold. Nup205 connects the short arms of the two Y complexes and associates with the stem of a neighboring Y complex. The Nup214-containing region uses an extended coiled-coil to link Nup85 of the two Y complexes and protrudes into the axial pore of the NPC. These previously uncharacterized structural features reveal insights into NPC assembly.
History
DepositionDec 18, 2019-
Header (metadata) releaseJul 21, 2021-
Map releaseJul 21, 2021-
UpdateJul 21, 2021-
Current statusJul 21, 2021Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6lk8
  • Surface level: 0.03
  • Imaged by UCSF Chimera
  • Download
  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-6lk8
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_0909.png

Downloads & links

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Map

FileDownload / File: emd_0909.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationcore region reconstruction
Voxel sizeX=Y=Z: 2.222 Å
Density
Contour LevelBy AUTHOR: 0.03 / Movie #1: 0.03
Minimum - Maximum-0.0906904 - 0.15094903
Average (Standard dev.)6.626427e-05 (±0.0058456706)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 568.832 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z2.2222.2222.222
M x/y/z256256256
origin x/y/z0.0000.0000.000
length x/y/z568.832568.832568.832
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS256256256
D min/max/mean-0.0910.1510.000

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Supplemental data

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Sample components

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Entire : Cytoplasmic Ring Subunit of Xenopus laevis NPC

EntireName: Cytoplasmic Ring Subunit of Xenopus laevis NPC
Components
  • Complex: Cytoplasmic Ring Subunit of Xenopus laevis NPC
    • Protein or peptide: MGC83295 protein
    • Protein or peptide: Nuclear pore complex protein Nup85Nuclear pore
    • Protein or peptide: MGC154553 protein
    • Protein or peptide: Nucleoporin SEH1-A
    • Protein or peptide: outer Nup160
    • Protein or peptide: MGC83926 protein
    • Protein or peptide: Nuclear pore complex protein Nup96Nuclear pore
    • Protein or peptide: GATOR complex protein SEC13
    • Protein or peptide: Nuclear pore complex proteinNuclear pore
    • Protein or peptide: outer Nup133
    • Protein or peptide: Nup358 complex, clamps
    • Protein or peptide: Nup214 complex Coiled-coil region 1, helix 1
    • Protein or peptide: Nup214 complex coiled coil region 1, helix 2
    • Protein or peptide: Nup214 complex coiled coil region 1, helix 3
    • Protein or peptide: Nup214 complex Coiled coil region 2, helix 1
    • Protein or peptide: Nup214 complex Coiled coil region 2, helix 2
    • Protein or peptide: Nup214 complex Coiled coil region 2, helix 3
    • Protein or peptide: bridge domain

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Supramolecule #1: Cytoplasmic Ring Subunit of Xenopus laevis NPC

SupramoleculeName: Cytoplasmic Ring Subunit of Xenopus laevis NPC / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Xenopus laevis (African clawed frog)

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Macromolecule #1: MGC83295 protein

MacromoleculeName: MGC83295 protein / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: African clawed frog (African clawed frog)
Molecular weightTheoretical: 227.854141 KDa
SequenceString: MAAQLALNSE ASLWGPYREI WQTVLSALIK RQPEAVHSLD IVLKKYKPDF ISLFKNPPKS AQQHERVQKA STEGIPIKGT QRTRILEEQ LIKEAFILSD LYNIGEIAAV ELLLIGEQQQ PTFHGLTRGL VAILLYWDGK SCMAESLLHL IQARKGKTFT L DHSPEVVS ...String:
MAAQLALNSE ASLWGPYREI WQTVLSALIK RQPEAVHSLD IVLKKYKPDF ISLFKNPPKS AQQHERVQKA STEGIPIKGT QRTRILEEQ LIKEAFILSD LYNIGEIAAV ELLLIGEQQQ PTFHGLTRGL VAILLYWDGK SCMAESLLHL IQARKGKTFT L DHSPEVVS MVTRFTDDLM EQGLTNKILT LISQIDVNNE FDKLKKERGL GNKKHRKEVS DLIKECQQSL AHSLYSWSCQ TP LNREDTL LLIGYLEKVT VEGDGSLDKV NLTLLMSLLY CLDVGFLEQG TDDREELMKQ ASMFMDRQYI AAIHNRLQNT QPW KSPGMQ ATVRLAWALA LRGISQFSEV LEFSEADEPM AEIAIGGNVF LFLTEAVVGS ESFCTDEFFI RRIHKLVTDF PTLM PMKVK QLRNRAEEDA RLIQMSMQMG NEPPASLRRD LEHLLLLIGE LYRKDPFHLE LALEYWCPTE PLQSTSLMGS FLGVA HQRP PQRQVLLSKF VRQMSDLLPA TLYLPYLKML RGLASGPQCA HYCFSLLKAN GGSSAENLQA AGGSPVSWDH FFHSLM LYH EHLRRDLPNT DNIHQRHPPL RGITQRELDG LIACLQLTCT IIDWSESARL ALCEHAQWMP VVVILGLLQC SIPPLLK AE LLKTLAAFGK SPEIAASLWQ SLEYTQILQT VRATGLRQGV GIEVELNEIE SRCEEYPLTR AFCQLISTLV ESSFPTNL G AGLRAPGFEP YLQFLRDTVF LRYRTRAYRR AAEKWEVAEA VLDVFYKLLK DYEPQPEDFV DQYVELQGEE RVAFKPPGF SLMHHLLNES PMLELCLSLM EEGVTQLDTY APFPGKKHLE KAVAYCFMLL NLTLQKENRF MDLLRESHLS MIVTPLEQLL QGINPRSKK ADNVVNIARY LCHGNSNAEL AFESAKILCS ISCNSKIQEK IVGDFTQDQN VSQKLMVGFV SCLDSEEAEE L LDSEKEAE DQVKQTNIRY MTKIHILNLL ITSLEMKAPN LAMFLLGYEL KKPVSTTNLQ DSGVLGCPRT CLHSILDILR KG TDVRAGP VAVWDTPHLA ELCYQVIYQL CACADTSGPT MRYLRTSQDF LFSQLQHLPF SVEESEISAM NQMSWLMKTA TIE LRITSL NRQRSHTQRL LHLLLDDMPT RPYSADGEGG MEDESRSLSG FLHFDTTSKV RRKILRILDS IQFSNEIPEP LQLD FFDRS QIEQVIANCE HKNRRGQTVC NVKLLHRVLV AEVNALQGMA AIGQRPLLME EINTILQYVV ERNKLLQCLH AKRHA LESW RQLVEIILTA CPQDLIPTEH RQLIIRDLLQ DLHVKILDDD AAQELMPIVA GAVFTLTAHL SQSVRTELKQ PMTASG LGQ SQYVQMLDGS FAAPPGTENI SAGFASIGDS SLHMILRNLL EFILKTGGGF QRVRAHLYGS LLYYLQIAQR PDEPDTL ES AHKSMWERLT APEDVFSKLQ RDNLSIFESY GTALMEVVCR DACDGHDIGR MLALALLDRI VSVDRQQQWL LYLSNSGY L KVLVDSLAED DVVLRNLLTP QPPLLKALYI YESKMAFLTR VAKSSQGAIE LLRSGVIVRL AQCQVYDMRP ETDPHGVFG MRETPVFIPA PVERYRQILL PALQICQLIL TSSTAQHLQA AGQVLQFLVA HSDTIQAILR SQEGSLGSLQ ELALLTGIIS KAALPGVLN ELDIGLNDGS MMELQGHIGR FQRQCLALLN RFGGSDRLRQ LSLQDDSSRL DGVSKKDDME LAMQQICSNV M EYCQALMI QNSPSFQQTV CLFTPSLKES ASRDGTRQDS QVSILPSWRL PSLGVVIHLL KQSANNFFTY YDIHRQSVGK LQ NVEQLPP DEIKELCQSE MPVGADKIST TQKYGLARRR LVKLINSRAK LLSLCSYIIE TCLYILWRHL EYYLLHCTTS DSQ DPVFSN MTFGNRRFQD TFNTDPNMDP RNLRQNKVSQ QDVDTLLREG ANSFGESLQK RLLDIESLYC KVRSRHSFIQ ALVR RIRGL LRVSRV

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Macromolecule #2: Nuclear pore complex protein Nup85

MacromoleculeName: Nuclear pore complex protein Nup85 / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: African clawed frog (African clawed frog)
Molecular weightTheoretical: 75.160047 KDa
SequenceString: MEELDVDPAE TPIPGLGQQN RHIGFSWGPG DLLLYETLYQ KQGNSETAAR CPFMYLVRSD EDIYSPVLRK LFNESHSIFV GLQKSAEEA SGKSRKAQLV QVSRNYRSVL RACMEEMHTL SESTRETAQK YISQISILSA MELSWNLCEI LFIESAPAGP L LILLLEWV ...String:
MEELDVDPAE TPIPGLGQQN RHIGFSWGPG DLLLYETLYQ KQGNSETAAR CPFMYLVRSD EDIYSPVLRK LFNESHSIFV GLQKSAEEA SGKSRKAQLV QVSRNYRSVL RACMEEMHTL SESTRETAQK YISQISILSA MELSWNLCEI LFIESAPAGP L LILLLEWV RLHVCEVDNI VQDVLRSEKP TEHEKFWDGV TGYVLQGRMN EARQLLAKEA STSASARSMC RVLDDLLKKM PM LHTGGTQ TLTEFELKWQ HWREECERHL QNGTFSSNVH MEAVCRVLLG DEEVLLEKRD LMTTWYHFLV SRLLFKHPTV KPT ELHFYA QSSLDMFLAG DSCPEPLDNI LLAAFEFDIH QVIKEFSIVS SNWWFVAHLT DLLDHCQLFQ AHNLYFGANM REFL LLDYA SGLFSHHSLW QLGVDYFDYC PNLGREYLKL HMERIPLSTE KKALKALRIC EQRQMTEQVR SICKTMAMQS LCNRR LGSA LSWSIRAKDA AFATLISDRF LKEYCERGNF TDLDLIDNLG SAMLLSDRLT FLGKYREFHR MYSQEQFSEA ASLLLS LMT ARIAPCSFWL TLLLDALPLL EQKQVIFSAE QTYELMRCLE DRMAAKLEST SPDEIQKQDS SIDNTKVEML RLALARN LA RAIVTEGALQ E

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Macromolecule #3: MGC154553 protein

MacromoleculeName: MGC154553 protein / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: African clawed frog (African clawed frog)
Molecular weightTheoretical: 41.744512 KDa
SequenceString: MADKFAAKFV SHKISRTRWR PVSASSLQQP DVFATGSWDN EENKVCVWAT SDFGATSLDE EYQGDPKQLC DIKHPGDVMD MQFLDKERI VTGSSTGTVT IFRHHENNQT LSVNQRWEQA HYHVGSNMRA PCTAIVCSSP EIVSVGEDGR INCFRAESRD V LRTIDDAD ...String:
MADKFAAKFV SHKISRTRWR PVSASSLQQP DVFATGSWDN EENKVCVWAT SDFGATSLDE EYQGDPKQLC DIKHPGDVMD MQFLDKERI VTGSSTGTVT IFRHHENNQT LSVNQRWEQA HYHVGSNMRA PCTAIVCSSP EIVSVGEDGR INCFRAESRD V LRTIDDAD SSTMHGVTFL RTTEILTVNS VGQLKLWDLR KQGNDPTQIF SVTGERVPLH CVDRHPNQQH VVATGGQDGM LC IWDVRHG KMPMSLLNAH EAEMWEVHFH PSNPDHLFTC SEDGSLWHWD ASADSEKPTF LLGGRSTFNI SRSSIAPPNA NQS LACAWL STDPTKGQLE ITNLLPSSTL SVNSLDVLGQ NLVCGTDAEA IYVTRRLFS

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Macromolecule #4: Nucleoporin SEH1-A

MacromoleculeName: Nucleoporin SEH1-A / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: African clawed frog (African clawed frog)
Molecular weightTheoretical: 36.037664 KDa
SequenceString: MFVARSIAAD HKDLIHDVSF DFHGRRMATC SSDQSVKVWD KSENGNWHCT ASWKTHSGSV WRVTWAHPEF GQVLASCSFD RTAAVWEEI VGESNDKLRG QSHWVKRTTL VDSRTSVTDV KFAPKHMGLM LATCSADGVV RIYEAPDVMN LSQWSLQHEI S CKLSCSCI ...String:
MFVARSIAAD HKDLIHDVSF DFHGRRMATC SSDQSVKVWD KSENGNWHCT ASWKTHSGSV WRVTWAHPEF GQVLASCSFD RTAAVWEEI VGESNDKLRG QSHWVKRTTL VDSRTSVTDV KFAPKHMGLM LATCSADGVV RIYEAPDVMN LSQWSLQHEI S CKLSCSCI SWNPSSSRAH SPMIAVGSDD SSPNIMGKVQ IYEYNENTRK YAKAETLMSV SDPVHDIAFA PNLGRSFHIL AV ATKDVRI FTMKPLRKEL SSSGGVTKFE IHTVAQFDNH NSQVWRVSWN ITGTVLASSG DDGTVRLWKA NYMDNWKCIG VLK GD

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Macromolecule #5: outer Nup160

MacromoleculeName: outer Nup160 / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: African clawed frog (African clawed frog)
Molecular weightTheoretical: 162.658234 KDa
SequenceString: MAAAERHMTP FQAIDWAGSI TLPMVQRVGG FTRAIMAASV NLERSYMELI GAERETSRRN FRDLSLRPDV NLVIGGPKYA DCAGGYCYS ESSSLLSATR NRFLHWTSYA DTLELVEISL DINLVNNAVR LRILNCSILP GGVHICETPN NIVVLILTNQ T VHRLILPH ...String:
MAAAERHMTP FQAIDWAGSI TLPMVQRVGG FTRAIMAASV NLERSYMELI GAERETSRRN FRDLSLRPDV NLVIGGPKYA DCAGGYCYS ESSSLLSATR NRFLHWTSYA DTLELVEISL DINLVNNAVR LRILNCSILP GGVHICETPN NIVVLILTNQ T VHRLILPH PSRMYRSEII SDSHIQSIFT DIGKTNFHDP SNTYVIPAIP GRAPNTTAST AWLSSDGEAL FALPSISGGI LV IKMPPHD MEGLVTIAEL KQSSVMQRLL TGWMPSSIRG DQGPAHLPVS LAVHTLDHDS YLFALCQDHK LRMWSYKDQM CLM VADMLE YVPVSKDIRQ TAGTGHKLRL AFSETLGILY LGVYLHTPKQ GQFCVFQLMC AESNRYSLDH ISSIFTNQET LIDF TFTLT SMDIWALWLD DDNQTVVKHI NFEENQAGQW NPVFVNPLPE DDLAISDEQE PQEAYLECLF APGRFTIAAV QKAIQ ILRK GSGRVLDLSW EELRKDVTLT VENEIQNAVI DYDVSQEEFR QINIENWCKF YTCCLQYQET LSRPLALLVH PDTNMV CLL RKGFLSFLAP CSLVEHLYLV PAEHLLTVDE SVISDDIDAA SDIVNLIQCL RMIADYISED MAYLMESACC HLQSPER VA EQILEDLIAN DIDNIMENIQ NKLQDTRNPI RAIGFLLQNM DYETNADMEQ PQPNTRLNLS TLYGSITASS VVCQAICK I SATRFLICRD LLILQHLLLR LGDMALIGAG QLLHSQQELI PRAAQLLLSY YMIRWGSQCL ACAVPVDILE SNLQHLSVL ELSDSQVEKR RYTSGIQTIV ELFFEDVARK HFPHVFIQSG ASQLQEPLNW SDLIKRITNY LLQLLWPSNP NFQFAECLMR NCQYTQLQE YVRLLLPWCQ VNVGSCHFML AQCYLVAGEG HKALDCFSQA ASEVEREDFL EKLIRVEEGE SVSPRLQYYN R VLRLLEDV GLPELVIQLA TIAIGEASDD WRSQAALRTR IFKHHLDMGH NNQAYDALTQ IPDPSRQLDC LRQLVVVLCE RS QLQDLVE FPYVNLHNEV VGIIESRARA VDLMTHNYYE LLYAFHIYRH NYRKAGSVMF EYGMRLGREV RTLRGLQKQV NSY LACLNC LRLIRPEYAW IVQPVSGAVY ERPGASPKRN YDGESSAVPS SSQIEILELR DLEKEYVLAQ TRLTLAKHNP STAA IAGSS AAEEMVALLV QAGLFDTAIS LCQTFKLALT SVFEGLACKC IRLQQGGEAA QAEAWEWLAA NQLATVITTK ESSAT DEAW RLMISYLDKY EAKNTLYHHC IINKLLSHGV PLPNWLINRY KAMDAAELLR LYLKYDLLEE AAELVLEYVD ALLGKG HQY FGIQAPLSAT SQLVWFPYSA IDHLRQALGE NESNQHNQAI LSKLQRKMDE YFQKLKKATD DYKKLVQKPL RA

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Macromolecule #6: MGC83926 protein

MacromoleculeName: MGC83926 protein / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: African clawed frog (African clawed frog)
Molecular weightTheoretical: 36.588625 KDa
SequenceString: MKQDSASNAT YTVDCEDYVH VVEFNPFDSG EAGSLLAYGG ISYVVIASCR FQEEDSTVEG IEFKTLKTFH HGERVVAIAW SPETRCDAL LPLLRFATAA GDKKIRIFTS DFQDKNEYKV IEGHSGYIND LVFCSPEGTD IASVGDDHTC RIWDLDGKQI A MFILRSPG ...String:
MKQDSASNAT YTVDCEDYVH VVEFNPFDSG EAGSLLAYGG ISYVVIASCR FQEEDSTVEG IEFKTLKTFH HGERVVAIAW SPETRCDAL LPLLRFATAA GDKKIRIFTS DFQDKNEYKV IEGHSGYIND LVFCSPEGTD IASVGDDHTC RIWDLDGKQI A MFILRSPG MSVAWHPEGA FKLMVAEKTG TIRFYDLTTH QAILSLESVQ VPLMSADWCV RNTLRIGAVA GNDWIIWEMP RS SYPQDNK PAHADRARMF RWSKCNENVF ATTGYPGKMK SQIAIHHLAH PQPILIGTAP VGSGLSWHRR LPLCVVGGYR KLF FWLTEM

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Macromolecule #7: Nuclear pore complex protein Nup96

MacromoleculeName: Nuclear pore complex protein Nup96 / type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: African clawed frog (African clawed frog)
Molecular weightTheoretical: 105.079148 KDa
SequenceString: SKYGLQDSDE EDDQLNNAEA KKLKAAPVPP QGKPPPLQQA TLPGKVTPPP QSPAVDQLDR VLELDSDMAD ITQDQDLDSV AEEQDITEE QEPLSASSHI ASSLGINPHA LQVMKASLLL EEEDGEMINR FSSFPSSMDP YPDVRSPRLF PSSHAKRTSS M GLLQSKFA ...String:
SKYGLQDSDE EDDQLNNAEA KKLKAAPVPP QGKPPPLQQA TLPGKVTPPP QSPAVDQLDR VLELDSDMAD ITQDQDLDSV AEEQDITEE QEPLSASSHI ASSLGINPHA LQVMKASLLL EEEDGEMINR FSSFPSSMDP YPDVRSPRLF PSSHAKRTSS M GLLQSKFA SPSISRISET AQGSHSPRIL PVTPWSVPAP LAPTFVIPRP APETHLRTVG TRRQQELVPL EKSVTHGRGS LL IDMGLFM GRSFRVGWGP NWTLVHNGDK LTERLNAEED QNMDTIDYGF LPKPTSAKSL TESPFKVHME KLSLEQKSRE LQS YLMPLE IELKNSSVDR SAQCPHFKPN AGVAAIHDYA GWVRNLSNEA GELEAVVKQW GLTWTLCESL WGQLKELEAS LDEP NEYVR NLERRKAFSH WLAHTAEERI EEEVSLYGPE RHVEAVFSFL TGGRISDACR LAQKSGDHRL SLLLSQMVGS QEMRE LISL QLVDWNKLQV DHYIQEERLR VFCLLSGTPV WRSSDNRSIN VCSQLDWKRT LAVHLWYMLP PTATIAQALR LYERAF QEH EEGEPYACYP LPPYLEDCSI SLGDEPSAKF SSLQRDVCVH LLKLYSERQY DLCQLLDPSS VTPDPLDYRL SWHLWMV LQ ALNYTHLSEH RQGTLHASYA AQLENVGLWE WAIFVLLHIP HPHIREAGVR ELLNRQCVVR ESPESLAKEN FLIHRLCV P AQWVHEAKAI RSRRDGDRHK EALYLLKGHQ WNPCHKLVTR HLAADAVINE NYRYLQSFLG ELSNPEHCKH IQDWETAGK VYLDYIRVID MLNLIQQDES SGCELEKLHT KVMSLCKWVE LIHCYTAKDR LAQSEMAKRV ANILRVVLSL QQPPESMSDS SEPRVPLRL LAPHIGRLPM PEDYALEELR GLTQSYLREL ICDS

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Macromolecule #8: GATOR complex protein SEC13

MacromoleculeName: GATOR complex protein SEC13 / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: African clawed frog (African clawed frog)
Molecular weightTheoretical: 35.315285 KDa
SequenceString: MVSVINTVDT SHEDMIHDAQ MDYYGIRLAT CSSDRSVKIF DVKNGGQILI ADLRGHDGPV WQVAWAHPMY GNILASCSYD RKVIIWKEE NGTWEKTYEY TGHDSSVNSV CWAPHDFGLV LACGSSDGAI SILTFTGDGP WEVKKISNAH TIGCNAVSWA P SVIPGSLV ...String:
MVSVINTVDT SHEDMIHDAQ MDYYGIRLAT CSSDRSVKIF DVKNGGQILI ADLRGHDGPV WQVAWAHPMY GNILASCSYD RKVIIWKEE NGTWEKTYEY TGHDSSVNSV CWAPHDFGLV LACGSSDGAI SILTFTGDGP WEVKKISNAH TIGCNAVSWA P SVIPGSLV DQPSSQKPNY IKRFVSGGCD NLVKIWREED GQWKEDQKLE AHSDWVRDVA WAPSIGLPTS TIASCSQDGR VY IWTSDDA ATNCWTPKLL HKFNDVVWHV SWSITANILA VSGGDNKVTL WKESVDGQWA CISDVNKGQG AVSTVTEGQL NDQ

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Macromolecule #9: Nuclear pore complex protein

MacromoleculeName: Nuclear pore complex protein / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: African clawed frog (African clawed frog)
Molecular weightTheoretical: 105.398547 KDa
SequenceString: MDMLSPVVRE AEVSRAARRQ SSNRKNPADE SWSNATPTRG PSSRTTGQTL FRQHMTPQTW NSSRPPDVSA ILGTVGRSPR LLQTPGRLA NLSMMSNPDD SVWTTTFSPG RTGMYTTLDS PSFTEDITLS AVMLQEEDPG EAATMSMYPD FLKSFLEHPS S AVFELIEQ ...String:
MDMLSPVVRE AEVSRAARRQ SSNRKNPADE SWSNATPTRG PSSRTTGQTL FRQHMTPQTW NSSRPPDVSA ILGTVGRSPR LLQTPGRLA NLSMMSNPDD SVWTTTFSPG RTGMYTTLDS PSFTEDITLS AVMLQEEDPG EAATMSMYPD FLKSFLEHPS S AVFELIEQ YEATCNTQIT LLKKIVKRVT PGQQKFSKTA SILWLLQQEM VTWRLIAALY RDRIQSALEE ENMFEIAAPN AS EKTIVDK LFQRDTLVRQ SQLVVDWLES IAKDEVGDFS DNIEYYAKSV YWENTLHTLK QRSMLSLGSS RPLVSELDPD API RQKLPL DDLDREDDIR LLKYLFTLIR AGMTDEAQRL CKRCGQAWRA ATLEGWKLYH DANINGGTEL QAVEGNPYRC VWKT CCWRM AEDEQFNKYE RAIYATLSGN LKQLLPVCES WEDTVWAHFK VMVDSLVEQE IRASIISFNE ANELPREYLE ANWTL DSVF EELQATDKKR VLEENREHYH IIQKFVILAD VDGLMDEFSE WLSNGKNLLL GHLLRFMTHL LLFFRTLGLQ AKEEVS VEV LKTYIQRLIN EKQIELIAFY VSHLPQELAI SQYAVFLENI TDPDQRQRCL ELAKEAGLDV ASITKTVVEN TRKKDAG EF AHHDFAPALD SGTSEEDRAK IDVIDWLVFD PAQRAEALKQ SNAIMRKFLA SKKHEAAKEV FAKIPQDSIA EIYSQWEE Q AMDSALPAED DNAIREHLCI RAYLESHEAF NEWFKHINSP PQKPTLVGQA SFTEKVAHEH KEKKYEMDFG IWKGHLDAL TSDVKEKIYN VLLFVDGGWM VDVREDTEED PERSHQMVLL RRLCLPMMCF LLHTVLHNTK QYKDCLRLAD IVSSENQKLY TVFSKTEMR NLLQKLRESS LMLLDLQLDP LGYEIQS

+
Macromolecule #10: outer Nup133

MacromoleculeName: outer Nup133 / type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: African clawed frog (African clawed frog)
Molecular weightTheoretical: 127.55125 KDa
SequenceString: MFPSPRAQGM GSARRPFNSR LTGGRKALGP GVTASSSPSA LYSPVGRRVS ASGARSTPSR VYLHPAASET VNYNVQLFGS SLPVKVMEA LSNASADEPM AACIHEGGWA WLACNDRLII WKISHSSSAK LMVCKELPLP LSDSEWSADL VDICAQTGDP A AAQSVALM ...String:
MFPSPRAQGM GSARRPFNSR LTGGRKALGP GVTASSSPSA LYSPVGRRVS ASGARSTPSR VYLHPAASET VNYNVQLFGS SLPVKVMEA LSNASADEPM AACIHEGGWA WLACNDRLII WKISHSSSAK LMVCKELPLP LSDSEWSADL VDICAQTGDP A AAQSVALM AATPEGSSRY WPNILHEGTY IESYTEFGSS LCAFVTAVKG NSFILSSEKN QLVRLTPDAS GKMNQRVLPQ GQ GMLSGIG RRVSTLFGIL SPAVESTLCS VLWDKGDCFY TLTDSSINKW DLDDTSESQV LNWDMSRVLR EYISDAIWGS ESD YDDIKA GININYLSLN QNCDGLVILS AAWHPGDNPC QIYYTLVTVK DEGYNISDEI TVEVTQFNPV FQARGMQLCQ LVVP NFSSQ ACYLYTQEMI FACSTGTGRS TLPQEKIPFE AQGDNIVGAG SCEGWPVFFI RKSGMLTVVA RETASVLPEH MEESL SSVS KSSRQAVVKD SRPDQIAHDD KTKHLKAAFL RYCRKDILGA QSMVDSLFSD SDMEPDDELD LAVNQISVDL IDDYPA SDP RWAESVPEEA AGFSNTSLIL LHQLEDKMKA HSFFVDFLHQ VGLFSRLSTC QTKGMLVATR LLLSEHAEKL SAAIVLK NH HAKLPVLVNS AIQLALDKRM CTVPQNLTAA DVYFREVSQM EIIFECLVDK EEADLESTSI DSVEWANIVV NVNTILKD M LHVACQYRQS KNSLYKNESG IQEPEHVPWT ASSGTAGIRS VVTRQHGIIL KVYPQADSGL RTILIEQLAA LLNYLLDDY VTQLKSIDKL ANEERYNILE MEYAQKRSEL LSPLLILGQY AWASNLAEKY CDFDILVQIC EMTDNQSRLQ RYMTLFAEQN FSDFLFRWY LEKGKRGKLL SQPASQHGQL AAFLQAHDHL SWLHELNSQE FEKAHRTLQT LANMETRYFC KKKTLLGLSK L AALASDFQ EDVLQEKVEE IAEQEHFLLH QETLPKKLLE EKQLDLNAMP VLAPFQLIQL YVCEENKRAN ENDFMKALDL LE YIGDDSE VDVEELKLEI LCKAIKRDEW SATDGKDDPI EATKDSIFVK VLQNLLNKGI ELKGYLPKAE TLLQSEELNS LKT NSYFEF SLKANYECYM KMQS

+
Macromolecule #11: Nup358 complex, clamps

MacromoleculeName: Nup358 complex, clamps / type: protein_or_peptide / ID: 11 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: African clawed frog (African clawed frog)
Molecular weightTheoretical: 322.784344 KDa
SequenceString: MRRSKAEIQR YVENAQNSAS SPREKSMKGF LFARLYYEAK EYELAKRSVS SYISVQERDP KAHRFLGQLF EIEGNVEKAV GCYKRSLEL NPTQKDLTLR IAELICTLNI KDGRAEYWVE RASKLFPGSP EIYRLKEQLL SSQGEAGWNQ LFDLIQAELF A RPNDVYVN ...String:
MRRSKAEIQR YVENAQNSAS SPREKSMKGF LFARLYYEAK EYELAKRSVS SYISVQERDP KAHRFLGQLF EIEGNVEKAV GCYKRSLEL NPTQKDLTLR IAELICTLNI KDGRAEYWVE RASKLFPGSP EIYRLKEQLL SSQGEAGWNQ LFDLIQAELF A RPNDVYVN LKLVDLFLSN QRLEEAVLHC LKPERRALRT DIEWCSCVVR VFKEYLASKQ GQKNTNMRMI TKELLLAQCD VV FLTLSKK DVQKSKEALE RFDQALLSVK QSVSGTDASD LSVTFYEMRG HYYMHAGTLL LKMAQSCEVQ WKALIEPAAL CYL LAYQVP KPKSKPVKGD DNGQGFLEEL AFDRQSKSGH LLLTLSHGKQ NFISEIIETF ANQCGQSILL KFLFEDNLSM QDSF MGSDD ISYVENRVPD LSELSQHDNG SLRIHNGDLQ HLTWLGLQWH FLSTLPPLRK WLKQIFPRVP QETSRLESNI PESIC LLDL EVFLLAVVQT SYLQLQDNNT TADPNRPRCL PLPICKQLFT DRQRSWWDAV YSLITKKALP GTSAKLRSVI QHDLTT LRA QEKHGLQPAV LVNWARGLHK TGYSLNSFYD QKEYMGRCVH YWKKLLPLLD LVKQKKSIPE PVDPLFKHFH NKDIKVS EV KDLEDEACIA FATLDLVDGK TEDAIIAFES VKNVVAYWNL ALIYQRKAEE IENDCLPAEE QEEFQECLLK CKGFLKMI C DEYSAYPSIA TSLPVPVETV FEMLDSVKQS LGEAMDDHSP AFMENHSVLT TSAIKHSTPS PTKLTISPSK SARFSPKTP PRWAEDQKSL LEVLCNKVEA LKKEVQELKH NNSNANVSPH RWPNEGFESD TVADSYQGTQ NFYTVPLTVS TSGPAAYYGQ SPAYNSQHL LRPAANITPT KTSVYAMNRL PPQQHMYTYP QQMHTPPTQQ SSAGCVFPQE IYGPPLRFES PAAAILSPHN E EFYNYNVP PASTNPPLPE PGYFTKPSTA MQHSKQEVPK VSDFGKGCLG QSTSEGQKPS PFTVPMQSTP ASSTFKFNSN FK SNDGDFT FSSSHAGASS AYTGSESLLG LLTSDRPTQE QGKKSDFENI ASDEKNMFRF GEKSFSPGFT VTGTQSQDKN PLV FGQSEN IFTFKTPGKS TFKPPTFGTQ TKDAHNHSVE SDAGSEHVAD DDGPHFEPII PLPEKVEVKT GEEDEEEMFC NRAK LFRFD AETKEWKERG IGNVKILRHR LSGKIRLLMR REQVLKICAN HYINADMKLK PNATSDKSYV WHAYDYADEM PKPEQ LAIR FKTVDEAAHF KAKFEEAQRL LAMAEAPAIS AQHKNAKDNL KLDASKVKEA PLPFGSQFIL KRGEWQCDCC LATNAP TST SCVCCQTPNK NKSSSISSVC ISAPSFTFVK ESATNKLAFG QQLLKDKDQW TCSKCSQKND AGVSHCSSCQ TQSQAKA GI SQPNIASSGF TNNTSAQGDN LAAVFGKKAG QWDCDVCYVR NEPSANKCIS CQNTKPLSKV SGTQAASFSF AAGADNSQ K NFGAQFAKKE GQWDCDACYV RNEPLATKCI SCQNTKLLSK TTGTQAASFS FAAAADNSQK NFGGQFAKKE GQWDCSSCL VRNEASAPNC VACHSANPQI TNKDVVPPAL TPSGFKFGHN AEVGKTQQSL SAMFSCKQGQ WECSTCLVIN DAAKDTCAAC QAAKPGSSA SQSKEVPSTF GIKANSSQNF GQPAAGFNCG FSAKGFKFGI SDEKASASNF TFKAPATNEE TKMVKDGFNF P VSAGSLSF KFGISEPDKT KEMSTGFMKG TSTNNKGSET AETTAQAEKI QQSPDKVLGQ SVQSFSFADI AKSTEGIEFG KA DPNFKGF SGAGQKLFTS SNQVNASNAQ EAADDLYKTE ERDDIHFEPI VQLPDKVDLI TGEEDEKTLY SQRVKLYRFD ATS GQWKER GVGNLKILKN EVNGKLRVLM RREQVLKVCA NHWITTTMNL KPLTGSDRAW MWLANDFSEG DAKLEQLAVK FKTP EQAEE FKIKFDQCQC LLLDIPLQTP HKLVDTGRTA HLIQKAEEMK TGLKDLKTFL TDKAKPLDDS NAINTTDLEK QALAD GTEP TYEWDTYDMR GDAHEETLDD SVYASPLASS PEKKNLFRFG DLSTSGFNFS FQPEPSPSKS PTKLNHSRVS VGTDEE SDV TQEEERDGQY FEPVVPLPDL VEVTSGEENE QAIFCHRAKL YRFDKDSNQW KERGIGDLKI LQRLDNKSAR VVMRRDQ VL KLCANHRITT DINLQPMKGA ERAWVWTAHD FSEGEGKVEC FAVRFKLQEA ADLFKEVFEE AKEAQAKDCL LTPVSSRG T TPRAASCGKA AIAILEETTK ERTDQQPEED TSLTEASTPS PTDQPAKALV SPANFTFGSD VVKNIFGSEK QVPFAFGNT SSTGSLFGFS FNASQSQGQQ VQKQPPKVTL DFNATFKDAE TTNALQKPSQ SSGQSPIVSS LSSSSSSSSS TLMQPMPARD KAADVPDAD SSSDVLIVYV ATPTPEQKAL AETLLLPLTF FCYKNKPGYV SDESDIDDED FETAVKNLNG ILYTEDKKDK A SSRLSGCS KEPTAESDQD CIIVWEKKPT PEEKAKADSL KLPPTFFCGL GSDTDEDKDN LEDFDTEVRK VKEAKGVPEA DV TSSPEAA IVSAAETSVS LPPKQEPDST TSISQEPVDL SSKQELPKTD SKGFSTPSFS FGLGEVSGVS FADLASTNSG DFA FGSKDT NFQWANTGAA VFGTLSQNKK GEDADGSDEE VVHSDDVHFE PIVSLPEVEV KSGEEDEEIL FKERAKLYRW DRAV GQWKE RGVGDIKILF HKEKGYYRVL MRRDQVLKVC ANHVISTEIK ISTLSTSNNS LVWTATDYSD GEGKVEQLAV RFKTK ELTD SFQNKFEECQ HNLQEESNPQ H

+
Macromolecule #12: Nup214 complex Coiled-coil region 1, helix 1

MacromoleculeName: Nup214 complex Coiled-coil region 1, helix 1 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 5.890252 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)

+
Macromolecule #13: Nup214 complex coiled coil region 1, helix 2

MacromoleculeName: Nup214 complex coiled coil region 1, helix 2 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 6.826406 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

+
Macromolecule #14: Nup214 complex coiled coil region 1, helix 3

MacromoleculeName: Nup214 complex coiled coil region 1, helix 3 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 6.230672 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

+
Macromolecule #15: Nup214 complex Coiled coil region 2, helix 1

MacromoleculeName: Nup214 complex Coiled coil region 2, helix 1 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 2.656265 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

+
Macromolecule #16: Nup214 complex Coiled coil region 2, helix 2

MacromoleculeName: Nup214 complex Coiled coil region 2, helix 2 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 2.996685 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

+
Macromolecule #17: Nup214 complex Coiled coil region 2, helix 3

MacromoleculeName: Nup214 complex Coiled coil region 2, helix 3 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 2.230741 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

+
Macromolecule #18: bridge domain

MacromoleculeName: bridge domain / type: protein_or_peptide / ID: 18 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 33.293949 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statetissue

-
Sample preparation

BufferpH: 7.4
GridModel: Quantifoil / Material: COPPER
VitrificationCryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 75.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 5.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 616547

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-6lk8:
Structure of Xenopus laevis Cytoplasmic Ring subunit.

+
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