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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-0777 | |||||||||
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Title | The ClassA RSC-Nucleosome Complex | |||||||||
![]() | The ClassA RSC-Nucleosome Complex | |||||||||
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![]() | chromatin remodeler / ![]() | |||||||||
Function / homology | ![]() regulation of sporulation resulting in formation of a cellular spore / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Ye YP / Wu H | |||||||||
![]() | ![]() Title: Structure of the RSC complex bound to the nucleosome. Authors: Youpi Ye / Hao Wu / Kangjing Chen / Cedric R Clapier / Naveen Verma / Wenhao Zhang / Haiteng Deng / Bradley R Cairns / Ning Gao / Zhucheng Chen / ![]() ![]() Abstract: The RSC complex remodels chromatin structure and regulates gene transcription. We used cryo-electron microscopy to determine the structure of yeast RSC bound to the nucleosome. RSC is delineated into ...The RSC complex remodels chromatin structure and regulates gene transcription. We used cryo-electron microscopy to determine the structure of yeast RSC bound to the nucleosome. RSC is delineated into the adenosine triphosphatase motor, the actin-related protein module, and the substrate recruitment module (SRM). RSC binds the nucleosome mainly through the motor, with the auxiliary subunit Sfh1 engaging the H2A-H2B acidic patch to enable nucleosome ejection. SRM is organized into three substrate-binding lobes poised to bind their respective nucleosomal epitopes. The relative orientations of the SRM and the motor on the nucleosome explain the directionality of DNA translocation and promoter nucleosome repositioning by RSC. Our findings shed light on RSC assembly and functionality, and they provide a framework to understand the mammalian homologs BAF/PBAF and the Sfh1 ortholog INI1/BAF47, which are frequently mutated in cancers. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 20.8 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 38.3 KB 38.3 KB | Display Display | ![]() |
Images | ![]() | 72.2 KB | ||
Filedesc metadata | ![]() | 12.4 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6kw3MC ![]() 0778C ![]() 9905C ![]() 6k15C ![]() 6kw4C M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | The ClassA RSC-Nucleosome Complex | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 2.14 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
+Entire : RSC
+Supramolecule #1: RSC
+Macromolecule #1: Histone H4
+Macromolecule #2: Histone H3.2
+Macromolecule #3: Histone H2A
+Macromolecule #6: Nuclear protein STH1/NPS1
+Macromolecule #7: Actin-related protein 7
+Macromolecule #8: Regulator of Ty1 transposition protein 102
+Macromolecule #9: Chromatin structure-remodeling complex subunit RSC7
+Macromolecule #10: Chromatin structure-remodeling complex protein RSC8
+Macromolecule #11: Chromatin structure-remodeling complex subunit RSC9
+Macromolecule #12: Chromatin structure-remodeling complex protein RSC6
+Macromolecule #13: Chromatin structure-remodeling complex subunit SFH1
+Macromolecule #14: Chromatin structure-remodeling complex protein RSC58
+Macromolecule #15: High temperature lethal protein 1
+Macromolecule #16: Chromatin structure-remodeling complex protein RSC30
+Macromolecule #17: Chromatin structure-remodeling complex protein RSC3
+Macromolecule #18: Chromatin structure-remodeling complex subunit RSC4
+Macromolecule #19: Chromatin structure-remodeling complex subunit RSC2
+Macromolecule #20: Histone H4
+Macromolecule #21: Actin-like protein ARP9
+Macromolecule #4: DNA 167
+Macromolecule #5: DNA 167
+Macromolecule #22: ZINC ION
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | cell |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: DARK FIELD |
Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 2.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: EMDB MAP |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 7.13 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 45077 |