EMDB-0231: Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosom...

Basic information

• Entry: Database: EMDB / ID: EMD-0231
• Title: Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the large mitoribosomal subunit
• Map data: map of the T. brucei mitoribosome large subunit

Sample

• Complex: large subunit of the T. brucei mitoribosome
  • Protein or peptide: x 85 types
  • RNA: x 1 types
• Ligand: x 3 types

Function / homology

Function:

: / organellar large ribosomal subunit / mitochondrial mRNA editing complex / mitochondrial RNA processing / kinetoplast / nuclear lumen / mitochondrial large ribosomal subunit / ciliary plasm / mitochondrial ribosome / cyclosporin A binding / mitochondrial translation / post-transcriptional regulation of gene expression / ciliary basal body / peptidylprolyl isomerase / peptidyl-prolyl cis-trans isomerase activity / large ribosomal subunit rRNA binding / large ribosomal subunit / protein folding / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / mitochondrion / RNA binding / metal ion binding / nucleus / cytoplasm

Domain/homology:

Pentatricopeptide (PPR) repeat profile. / LIM domain / Pentatricopeptide repeat / Zinc-binding domain present in Lin-11, Isl-1, Mec-3. / Zinc finger, LIM-type / Ribosomal protein L46, N-terminal / 39S mitochondrial ribosomal protein L46 / LIM domain profile. / 39S ribosomal protein L46, mitochondrial / DnaJ domain / Ribosomal protein L47, mitochondrial / MRP-L47 superfamily, mitochondrial / 39S ribosomal protein L43/54S ribosomal protein L51 / Mitochondrial 39-S ribosomal protein L47 (MRP-L47) / Phosphatidylethanolamine-binding protein, eukaryotic / Rhodanese-like domain superfamily / PEBP-like superfamily / DnaJ molecular chaperone homology domain / dnaJ domain profile. / Chaperone J-domain superfamily / DnaJ domain / Cyclophilin-type peptidyl-prolyl cis-trans isomerase / ANL, N-terminal domain / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain profile. / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain / Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD / Cyclophilin-like domain superfamily / AMP-dependent synthetase/ligase / AMP-binding enzyme / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / NUDIX hydrolase-like domain superfamily / Zinc finger C2H2 superfamily / Ribosomal protein L11, N-terminal / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, N-terminal domain / Ribosomal protein L11, RNA binding domain / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / : / Ribosomal protein L16 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L21 / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein / Ribosomal protein L24 / Ribosomal protein L3, bacterial/organelle-type / Ribosomal protein L15, bacterial-type / 50S ribosomal protein uL4 / Prokaryotic membrane lipoprotein lipid attachment site profile. / Ribosomal protein L10e/L16 / Ribosomal protein L10e/L16 superfamily / Ribosomal protein L16p/L10e / Tetratricopeptide-like helical domain superfamily / Ribosomal protein L25/L23 / Ribosomal protein L30, ferredoxin-like fold domain superfamily / Ribosomal protein L26/L24, KOW domain / Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A / Ribosomal protein L22/L17 / Ribosomal protein L22/L17 superfamily / Ribosomal protein L3 / Ribosomal protein L13 / Ribosomal protein L13 / Ribosomal protein L13 superfamily / Ribosomal protein L3 / Ribosomal protein L22p/L17e / Ribosomal protein L4/L1e / Ribosomal protein L4 domain superfamily / Ribosomal protein L18e/L15P / Ribosomal L18e/L15P superfamily / Ribosomal protein L4/L1 family / Alpha/Beta hydrolase fold / Translation protein SH3-like domain superfamily / Ribosomal protein L23/L15e core domain superfamily / Thioredoxin-like superfamily / Translation protein, beta-barrel domain superfamily / Ribosomal protein L2, domain 2 / Nucleotide-binding alpha-beta plait domain superfamily

Component:

Large ribosomal subunit protein uL4m / Replication restart DNA helicase PriA / 39S ribosomal protein L55, mitochondrial / KOW domain-containing protein / 39S ribosomal protein L32, mitochondrial / LETM1 domain-containing protein / Uncharacterized protein / Chaperone protein DNAj, putative / TPR_REGION domain-containing protein / Large ribosomal subunit protein uL22c / Large ribosomal subunit protein bL21m / MRP-L46 domain-containing protein / T. brucei spp.-specific protein / Uncharacterized protein / Ribosomal protein L30 ferredoxin-like fold domain-containing protein / Uncharacterized protein / Uncharacterized protein / T. brucei spp.-specific protein / T. brucei spp.-specific protein / Uncharacterized protein / Uncharacterized protein / Mitochondrial RNA binding protein / T. brucei spp.-specific protein / T. brucei spp.-specific protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Ribosomal protein S24/S35 mitochondrial conserved domain-containing protein / Large ribosomal subunit protein bL20c / Uncharacterized protein / Peptidyl-prolyl cis-trans isomerase / Uncharacterized protein / Large ribosomal subunit protein uL23m / Zn_Tnp_IS91 domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Mitochondrial RNA binding complex 1 subunit / Large ribosomal subunit protein uL29m / Rhodanese domain-containing protein / Uncharacterized protein / Uncharacterized protein / Large ribosomal subunit protein uL15/eL18 domain-containing protein / Ribosomal protein L9 domain-containing protein / 50S ribosomal protein L16, putative / Peptidyl-prolyl cis-trans isomerase / Uncharacterized protein / Uncharacterized protein / AB hydrolase-1 domain-containing protein / Uncharacterized protein / 50S ribosomal protein L17, putative / Uncharacterized protein / 50S ribosomal protein L13, putative / Ribosomal protein L3 mitochondrial, putative / C2H2-type domain-containing protein / Uncharacterized protein / Large ribosomal subunit protein mL43 / Uncharacterized protein / AMP-binding domain-containing protein / Uncharacterized protein / LIM zinc-binding domain-containing protein / 39S ribosomal protein L28, mitochondrial / Uncharacterized protein / Large ribosomal subunit protein uL11m / Uncharacterized protein

• Biological species: Trypanosoma brucei brucei (eukaryote)
• Method: single particle reconstruction / cryo EM / Resolution: 3.39 Å
• Authors: Ramrath DJF / Niemann M / Leibundgut M / Bieri P / Prange C / Horn K / Leitner A / Boehringer D / Schneider A / Ban N

Funding support

Switzerland, 1 items

Organization	Grant number	Country
Swiss National Science Foundation		Switzerland

• Citation: Journal: Science / Year: 2018; Title: Evolutionary shift toward protein-based architecture in trypanosomal mitochondrial ribosomes.; Authors: David J F Ramrath / Moritz Niemann / Marc Leibundgut / Philipp Bieri / Céline Prange / Elke K Horn / Alexander Leitner / Daniel Boehringer / André Schneider / Nenad Ban / ; Abstract: Ribosomal RNA (rRNA) plays key functional and architectural roles in ribosomes. Using electron microscopy, we determined the atomic structure of a highly divergent ribosome found in mitochondria of , a unicellular parasite that causes sleeping sickness in humans. The trypanosomal mitoribosome features the smallest rRNAs and contains more proteins than all known ribosomes. The structure shows how the proteins have taken over the role of architectural scaffold from the rRNA: They form an autonomous outer shell that surrounds the entire particle and stabilizes and positions the functionally important regions of the rRNA. Our results also reveal the "minimal" set of conserved rRNA and protein components shared by all ribosomes that help us define the most essential functional elements.

History

Deposition	Aug 31, 2018	-
Header (metadata) release	Sep 26, 2018	-
Map release	Sep 26, 2018	-
Update	Nov 25, 2020	-
Current status	Nov 25, 2020	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_0231.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: map of the T. brucei mitoribosome large subunit
• Voxel size: X=Y=Z: 1.39 Å

Density

Contour Level	By AUTHOR: 0.1 / Movie #1: 0.1
Minimum - Maximum	-0.2243354 - 0.53609085
Average (Standard dev.)	0.0019741254 (±0.01902842)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 320 320 320 Spacing 320 320 320
Cell	A=B=C: 444.8 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.39	1.39	1.39
M x/y/z	320	320	320
origin x/y/z	0.000	0.000	0.000
length x/y/z	444.800	444.800	444.800
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	320	320	320
D min/max/mean	-0.224	0.536	0.002

Supplemental data

Half map: half map (odd) of the T. brucei mitoribosome large subunit

• File: emd_0231_half_map_1.map
• Annotation: half map (odd) of the T. brucei mitoribosome large subunit

Half map: half map (even) of the T. brucei mitoribosome large subunit

• File: emd_0231_half_map_2.map
• Annotation: half map (even) of the T. brucei mitoribosome large subunit

Sample components

Entire : large subunit of the T. brucei mitoribosome

• Entire: Name: large subunit of the T. brucei mitoribosome

Components

• Complex: large subunit of the T. brucei mitoribosome
  • Protein or peptide: bl27m
  • Protein or peptide: bl28m
  • Protein or peptide: ul29m
  • Protein or peptide: ul30m
  • Protein or peptide: bl31m
  • Protein or peptide: bl32m
  • Protein or peptide: bl33m
  • Protein or peptide: bl35m
  • Protein or peptide: bl36m
  • Protein or peptide: Ribosomal protein L3 mitochondrial, putativeRibosome
  • Protein or peptide: Ribosomal protein L4/L1 family, putativeRibosome
  • Protein or peptide: bl9m
  • Protein or peptide: ul10m
  • Protein or peptide: Ribosomal protein L11, putativeRibosome
  • Protein or peptide: 50S ribosomal protein L13, putativeRibosome
  • Protein or peptide: ul15m
  • Protein or peptide: 50S ribosomal protein L16, putativeRibosome
  • Protein or peptide: 50S ribosomal protein L17, putativeRibosome
  • Protein or peptide: bl19m
  • Protein or peptide: bl20m
  • Protein or peptide: bl21m
  • Protein or peptide: ul22m
  • Protein or peptide: ul23m
  • Protein or peptide: ul24m
  • Protein or peptide: ml38
  • Protein or peptide: ml40
  • Protein or peptide: ml41
  • Protein or peptide: ml42
  • Protein or peptide: ml43
  • Protein or peptide: ml46
  • Protein or peptide: ml49
  • Protein or peptide: ml52
  • Protein or peptide: ml53
  • Protein or peptide: ml63
  • Protein or peptide: ml64Microsoft Macro Assembler
  • Protein or peptide: bL12m
  • Protein or peptide: bL12m
  • Protein or peptide: bL12m
  • RNA: 12S rRNAMT-RNR1
  • Protein or peptide: ml67
  • Protein or peptide: ml68
  • Protein or peptide: ml69
  • Protein or peptide: mL70
  • Protein or peptide: ml71
  • Protein or peptide: ml72
  • Protein or peptide: ml73
  • Protein or peptide: ml74
  • Protein or peptide: ml75
  • Protein or peptide: ml76
  • Protein or peptide: Chaperone protein DNAj, putative
  • Protein or peptide: ml78
  • Protein or peptide: ml79
  • Protein or peptide: ml80
  • Protein or peptide: ml81
  • Protein or peptide: ml82
  • Protein or peptide: Peptidyl-prolyl cis-trans isomeraseProlyl isomerase
  • Protein or peptide: ml84
  • Protein or peptide: ml85
  • Protein or peptide: ml86
  • Protein or peptide: ml87
  • Protein or peptide: ml88
  • Protein or peptide: ml89
  • Protein or peptide: ml90
  • Protein or peptide: ml91
  • Protein or peptide: Peptidyl-prolyl cis-trans isomeraseProlyl isomerase
  • Protein or peptide: ml93
  • Protein or peptide: ml94
  • Protein or peptide: ml95
  • Protein or peptide: ml96
  • Protein or peptide: ml97
  • Protein or peptide: ml98
  • Protein or peptide: ml99
  • Protein or peptide: ml100
  • Protein or peptide: UNK
  • Protein or peptide: UNK
  • Protein or peptide: UNK
  • Protein or peptide: UNK
  • Protein or peptide: UNK
  • Protein or peptide: UNK
  • Protein or peptide: UNK
  • Protein or peptide: UNK
  • Protein or peptide: UNK
  • Protein or peptide: UNK
  • Protein or peptide: UNK
  • Protein or peptide: UNK
  • Protein or peptide: UNK
• Ligand: ZINC ION
• Ligand: NICOTINAMIDE-ADENINE-DINUCLEOTIDE
• Ligand: MAGNESIUM ION

Supramolecule #1: large subunit of the T. brucei mitoribosome

• Supramolecule: Name: large subunit of the T. brucei mitoribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#86
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Experimental: 1.9 MDa

Macromolecule #1: bl27m

• Macromolecule: Name: bl27m / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 21.793092 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLKCSLCYLA SKVTAGNAKN QAGHPRRKAK LFHVVPGTPV TPFEKLKEQR RRFGQDRHSR LPEYRPGNNV RMDPNTYTLY ATKKGVMTI RESRINPKYK WLDVEPDIQK VYRSRELRRA LQEREMASMA VGENSNYRVE LDLLLEPDWR ERVMHVPKAT E RFKDPNLF TRGVVNELSP LDRYSYT

Macromolecule #2: bl28m

• Macromolecule: Name: bl28m / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 28.051373 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLRNTDVRLF STFALNPETS VAPHGPPRGL VNRYVSMGLP PWAAWCNKVN RYSLYRMSGV TQRSFLPKPP QEMDVIWLNE RVRERVRTS RQVQNVYRQL KYPYVKTGIH YSDVLDHWVQ VPMVEAAMFE VEKDGGFDNF ILKRSGPELR STYGERIRRH I LVRQKEIQ KNFVLQKQAQ MLVESMEKEI LPMEDGKKVE EVLEKYGIDK EQLLRDIARA AVAKKQQLAS AVPQPSHVEV ST N

Macromolecule #3: ul29m

• Macromolecule: Name: ul29m / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 54.045367 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MQRNLTKLSA AAFYEFVDNN FLNNKRPPVP GGSWTVEVLR NKSLADLQHI WFLLLKERNM LKSMKEHYLR HQEELGAMPA PSRLKMIDE SMRNIKRVVK ERDEEATARA VEIFKERLKR GIYRYPPGPP PPPGAHDKTS VVKVELSCYV EEERLRELFG R YDVFEPHK GIVRVELKLP DEVLKQKEEA EQLWTQYMAE CSDVKAYHQW STAAPSAYDY TEVELAPGIF ANDAISDKGG KH SGDTETH EGVIVAARVP VPPPKEKQPP PKNPLERLKA ERRSYLARTT IQLGYFPNVT LPPPRYETVE AVPRPVHPDE IEG PWEAYI TYDREDGLSY AQSLGITTIG VATVLGLTEH VREPQPYAVV DPVYCEALRR ERAREETLMK WPHVPEWKYE YSTY TRKHL ADIVQYNYTN VVDYVDREVL LTGKSVWECP IHIDHTCGGS KTVPPHAKKP VRYMDAGIAN VGVTDI

Macromolecule #4: ul30m

• Macromolecule: Name: ul30m / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 25.204334 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MMRRMWVPTF SAQQQHLHHA MVLKMSTYAS TPSGNGVTSA ASNEEMNLHD AAAIAPGPYR RVGNIFIVHC DDHPFKHSWE VNRMLRELR LEFKGQTTIV PDIPQVRKRI WRVRHIVKVD VLDLDEAKAL IGVPEHISFT DLASQLPPSF GRVKAVPSPV I RSKMNFMK LRRMRLRDVL HRDALELRLL ELKRSAMKNA EQQKHAQKDE RTDNKEGEKC

Macromolecule #5: bl31m

• Macromolecule: Name: bl31m / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 21.083229 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MVLHKWAVVS RSAPPPRGLR PIARTIPTHP RLRPVDYKIP YVLRTFIKDR HTSEVQHLEN RGMFAEELSI ERSRFPRFHS TFTIQTDGS LNEREFEFAV PPIVTLFHDR LSAHRERQLE LAKIGKLRKE RNWETEQKGE ESVSMACNAL AFPYCIPKNM L KRSRVVDP LNSKSSTQGV TSGGG

Macromolecule #6: bl32m

• Macromolecule: Name: bl32m / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 9.74571 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MFRRTFFTPM IAQPTLLMLG NKGGTPKRKK NPMQLRRKVY GLHFKEKYLK MEEWYYCPLC AEPKKPGEWC RREDCRQIKP

Macromolecule #7: bl33m

• Macromolecule: Name: bl33m / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 13.04274 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLSSTALLLR GQYKTRLKKR MVGFIPKVIP RKIKNNMVAL RSEANTGHME GYLKTETERL DATGRKVQKV LWDPVLQRHC LFKETKIKG PFMTKSAIAK KVDFPIGGAE KMLKK

Macromolecule #8: bl35m

• Macromolecule: Name: bl35m / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 21.661453 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MGSEESNNIC AYKRTISLAK IYIVLLVKTA MLRYSRLCFP KMGCEEITRK ARRVQLQPTE YLAQHRMQVW QLRFKEMGPP FSRVWVALG GKMRRRRVGR QVDVKDMRYY WRPIEPQYQR LYMSRLRIRD HSNKLRQPMR LRATNADIGS GSSSIEWERA S NRKYGAML APPKRQDFEF RVV

Macromolecule #9: bl36m

• Macromolecule: Name: bl36m / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 21.795227 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MRGTTKPMLF LCSTAVRLNF FPLGLSAGPL NSHILLPRNN FDGRTTHGMK KIQGQSKDPQ MVMSRDELKL RCEYCRFEWI HDTLCVRCP AQPSHDQREM WLHSTWMWGK QQPYKYYKYM PAVRNPRTGM PMAREDARSM NNERRGQGLT TRTLNLEKER R GISRDVSR IGQYNTRWKT RFPFPT

Macromolecule #10: Ribosomal protein L3 mitochondrial, putative

• Macromolecule: Name: Ribosomal protein L3 mitochondrial, putative / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 55.073699 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MRSLIAMRRR FVSGTWTIAV STQQRHRSGS GPGDKRIRTD WYRCYPSLMR EKDRDMYHCY YPYLFDHGDK MSLYPKIPEN PREWQPEQL QTTYDAIRED KYDAFIRLRE KFPELYQDTR AWDNPPPFGE FNMFYSVRFG MVGVKAFTCK DYDELGNQFD C TAFWFPDN QVVKHSTRNG EVGTDKVYVG AMNVPVEFHK PHVAAFYKAA GVPVKHVCAG FPITPDAYAP VGTKLDVRHF KP GQEVTIT FQNTDYGFRG VMFRHGFDGG YVWLGDSRWQ RRPGAMGTEG QKRIYPGHRM AGQTGAAAET YQGVPVWRID YKN SLIYLP TLLDADVGTY VRFSDTINTK GLTLWNEHRG LPAFPTFIPP EDEDLSKLAT DECQLKSPPL YMYFRDEFPA TQLV SQADV EDAKSAKPAT APPKKKVYDM KKYYEARKKY RQSMQKARKY KLMGLRTKAH EKQEEARRAK ILKYKRMK

Macromolecule #11: Ribosomal protein L4/L1 family, putative

• Macromolecule: Name: Ribosomal protein L4/L1 family, putative / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 53.156664 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MRRVSFSVVL ATFGKRHSSI FSPRYDWRTS GVHDIAPRDE GDFLYQGPQH VLPGAHPLPL HHPHNTITRP VISPYIPSPQ RSHPYFTAP LPELPHFSTT KPIVYTYGTM KERIIAPVFN LKNEVIYTRE LDPFIFGMYP EVEELSKNLT YWMVRCQNFA S KWDYETRE IWRKAKKNWP NTGMGMPRVG NRKNHLYTWG GRTKPSKPWN MLMPTMDVKT WSKSNRMMLT LKMLQGRLQV VD RLTLEEP TQECYLELCR NMSWDVRHTG GGVLFMDGGS RITPSSEFDR AFFFGSFFNG RNKIVRPTVL CDEQYDYNKT AAK QRMKGP KGAKNPIPIN RFNAYDAMKH DRLVITEGAL MQLEDELYEH KLQILPPHIR NQLPEYGYLD SEALGDCVPS LKTI QMEAA ARTEEAESDM YKSFIDNPYN PWKDNMDASY AVDGADGTVQ KFVDGKKVSW SMLS

Macromolecule #12: bl9m

• Macromolecule: Name: bl9m / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 31.032697 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MRRTVVQRVA PWVPPPRHDI KVTMPPPPGG EVGGRFGVSQ GYSDRLARTP YWKRMALSTY KLRMMENATR YPMSEHRPGE YDIRYLPTP YPCTIRNRPL LEVGEPRQIP SIRIPVIFLV NLFDEAKGCW FGRRYETVYV ERQFMREELM PQRYAIYATP E AYKLLGLP VVNHHTHEEI PKTPREYEKL LERQRYDEER WKYTIEYLFR KYEDGPPELL DRPEDGWDGS EEIALSSVAG AG RDGVVSQ RKGPVKQRKA RKIKLF

Macromolecule #13: ul10m

• Macromolecule: Name: ul10m / type: protein_or_peptide / ID: 13 / Details: residues 106-129 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 20.283566 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MSPASPLPVA ALSRLRITHR SFLTRSRGRH VCRSAVGVEY RPEQQKKVLD HSYARVINAE VVHGDEQKFW GERRTFYTQR NIFFPMWDR CAQALILITR EVPRVPQEMA FRLMAVFLKL MLLPRLMMNT ELMLPMWIAS NAEGAMAAAK DGSKGKEQSS K QQGESKDD AKKEGDNTK

Macromolecule #14: Ribosomal protein L11, putative

• Macromolecule: Name: Ribosomal protein L11, putative / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 40.25275 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLRACGVLQL RARPKTVCVE PGSNRLPEAL VVEKARDIFG RPEFPGKRVL HNWRFFIKAG KAATGPPVGQ EFSKLGLKAM DFAKVFNDR TKPHFKEDVE LIVRIQVYFD KSYLFTIEPP PTAWFILRAL RKKRRETGPV PLRGHYCALM TLEMAYEIAK M KPLCWGRP EYPLLETRVR RVVGQARRMG VCFIGVDTPY SSPVKDMTEQ QYTEECERYR RIHMEQYTTL RQRELEEAPL IE RLHRPNM SPLTDEQIEE GLRDPCLLDT LWRASHPLSP YHRDLREREL ARRYLNARGW VKDMTPEEMR IVFMNYRLPE GEK RKQMDE AAMSGEVYWT RDGAQL

Macromolecule #15: 50S ribosomal protein L13, putative

• Macromolecule: Name: 50S ribosomal protein L13, putative / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 24.341309 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MQRSTRIVMA PFKSVLRRHK HHPEGLPLYN DMSKQWLCRE GERWWLLDAR GEQLPRVAAV AAQYITGQHR PDFTPGMITG DHVVIVNIK DVVMVGDQWI RVPITWQTAY PGGKYRIRLT DMYERDPCML MWWFLKDEVN RHFVRKLKTR IAPLEKAWLY E DSIHPHAD KNPRPLCWTD PEVKGWKYRD PQFIRRWKPN EFMH

Macromolecule #16: ul15m

• Macromolecule: Name: ul15m / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 43.780016 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLSVSPPLRR YRLFHPRREA IPMHMCPAKT IFPLINSNNL LVKTRNSWED FTGRKEFDED HPLPVVGSRL NGRTTQHKWN HWDQYLNPQ ITQSIKDLTP TPEYVGMRCG HNMIKMGWMK IGGSWKYSRG YNDRRRVFAR GQWQERKMTP RFMLAPRVSA G GPRNRYEG KLVFSPLRLS KLLWAIDTGR INPNEVITLY HLRQANVVGE REIVWPGFVL ISNGVRRVPY PIHIELQNAS AE SIRLIEE AGGSFTCVYM THEGLYQELH PEEYPIFMDQ ELPERRGLES LATNPSKRGW LTRWYEDSSK YAHPAAGRRY SHY LKPPTE RDFPATVEEY EMVKHHQKWH LNQPGTGTLL PWHSYNTADL VKRAAGRL

Macromolecule #17: 50S ribosomal protein L16, putative

• Macromolecule: Name: 50S ribosomal protein L16, putative / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 19.158145 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MPSPFQQTLL ARAYHYVHPK TGRPIRQFRR YGDPRYINNE AALIRGQWGL ITADFGMVTQ SQMENARLAI LRRLPRGSFT LTMHTDYEE FPVVKKSPES RMGAGKANIH HFAFKFTTGV PLFEIMPISA RRLNQAEAEG IFLAGRPFIP LQTVVVPQGR V DEYHVFK

Macromolecule #18: 50S ribosomal protein L17, putative

• Macromolecule: Name: 50S ribosomal protein L17, putative / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 35.117824 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLRITLLVVG RAPPVIGCTR KARYAGIDDN PTITYKPWDT TEPLMADYGW TRGKLPKFRA RSPFHRQQIA RRMVTEMIRK DYCIVGGAR APALRILADH VVELAKAGDT DSRQQLAYFL HDPLMVDKAF DEYPRRFKDM NAKYAMMTRL KSRRRTDAVA M YFVEYKNR DMSDNHKGED YSAGPERFFL PPRIVETEKG IQRPPHMQMA FDRWASKFKT EEFHHWWRLR HAKLRYWGVR NV PHPSDVD PLWTEKEEEE WHNEMLANTG EYEDLDLDDE SYTAAGEGGE PSPAGDGPQP QRVG

Macromolecule #19: bl19m

• Macromolecule: Name: bl19m / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 16.931455 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MGYTRERTNR HFFVARANAF FSRLPIARVQ RSLAMEAVRE GRMRPWKYTK EQILGAPVTC NFEYNPRPVR LIGTVMDAHT EETSIKGGL KVYARNEETN MMLWIPPGNP KLRHEVTSTK GSFQHYLDER DKWDEAWITG RARMK

Macromolecule #20: bl20m

• Macromolecule: Name: bl20m / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 24.393943 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLRRTVCVQH YRAKLELDRI RSMLRGRARL ERKVGLKRLF FLMRTQTRYR VEQQAHWERA IVRKNVDSAA REHGTGWQHL RNELGRQNV MLLPRSQQLL AQYEPLAFRA VVELCASRIP PPPPPVVASV PEESYTLWPP ASHDNSECAS TDGSDAPHGQ Q QSLSHPAA RVELRCGVER VLRRGPSGLG NNVNELIDAW KEFDVSPLRK GEVNK

Macromolecule #21: bl21m

• Macromolecule: Name: bl21m / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 20.895268 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MPSLANGLRC PDALITRTLN PLLNGPKFAV IYCGNHQYKV AVGDVIAVQR LRVEIGSHIA LKKVLMVGGP RFTAIGRPLL EGVRVTAQV EEQKRMRNVV SLFATPGRRH VRWVDAPHAA TIIRICEIFY SPNVVGELDK YKGELLETFT PGQHTNPVYP V DDGYDVFR KKDKEAMENA TTFLDLLGGV

Macromolecule #22: ul22m

• Macromolecule: Name: ul22m / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 32.083285 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MPRPAPRFAM GHMLPHRSNV GSQFLHTQRH GSRSTWYKKH YFSLRPFAIQ RHHGTTPRIL LDRSLWKSLW ITKLQLPDIN RWERVVNSR RVTEDRYAFV EEEGVMHKVN WGLYCERLET ELTVTQERLP QHTLLMKAVP SSWKKLDIDI SVIRGLSLRE A MAQCKLSL RKGHQIVFRA LEMAQQGAEA KGLDKEHLRV AHISCYPGPT DKQIDIRSKG YYAWKTKKSS HLVLTLAEDP EM VLPDRTC LPYSSLMSMK RAGLSAQPTV IDVPAITADG I

Macromolecule #23: ul23m

• Macromolecule: Name: ul23m / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 28.822936 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MVVSLRRFHS YANSSRGGAT TCLITGGGKG HFALTVSTRG RFYRPLVDDG INLWRRRMGR IHKGWRTWEY QHTRPDPRPF PDPPVNDYF GRSRIWNPIA CKLGYVRKKA DEWGWPNKPP PPTGLRHSQE FFPFFFERYF PDAEVRLLLD SVLNNETKRP V FQIPCSMS KVELVNYLKN IYGIDNVVRV EVRNRRGRRY KNEVGEIKMM DDYKVAVVEL DTPVSVELKQ IKGTEDTSDN RP QERITQ

Macromolecule #24: ul24m

• Macromolecule: Name: ul24m / type: protein_or_peptide / ID: 24 / Details: residues 313-340 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 43.999047 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MYARFRTRSK FYFRPARPAL AYNVDPNVMR RPKVKRGLLK GTYSDETVDL RDRERLELLE SMRHPRERDF YQDHTYHNQW LRRDLEKHQ KQQLAARYKY FAPDFEISPW IWYPGDIVEV VSGEGIGQRG TIIAVIKYKN EIVVQNINVQ DVVIPASESR P EQIVQREH PISVTRVRHV DPSTNEICNI EMVKVRNKET GEMEEKRMSL ESGILMSIPP VNDELEVGDP LKDTPIQDAD EA TYDREAE QAVLVDKRLE AMEEHFVQSL KQSYEFHEPL RRKNAEDMRQ FQTDVIDMAC AMLGERLLDT VNASDTSSFP AEW QEAIAM HVEEIEAEME EVAAKEMAEA KEGGAAENEQ HDDDLDDDED ELDTSEESVK HV

Macromolecule #25: ml38

• Macromolecule: Name: ml38 / type: protein_or_peptide / ID: 25 / Details: residues 41-53 and 294-311 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 58.437895 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MVMRRCSYNV HPTWYMMPLR AYHMPGWKTK VSTKDVRTRE QREADRKVRQ IEKLPRPQAL HDFQYPYEKT VLSDTMFQYP VYENELDTP HLFNITEPPD FFVYWNVSDF ERTSYPPLPE EDHRLLLPLS GSAPWKQHRK RSLKYLRKHE ILPNYLPHVR Q DVNLSVVF PGVYATRARL CEETGEPLPP PPPVSRMTHR NFWMTAHCGN YIELADLQHP PSIFFLDTVS ATGQGKAVEK DA TEEGSDE VWYTLIIASP DYPFRVPASV DASTQRGFFL NYMMSNLKGG GNSTVLEEYE SEQRQNNQKR QHLEELVRNP API AKEGDV IVSYTPPLPT EDAGTTRHIC MLFKQRSYVS GASCALDDSK ASFAARANFR LHAQHRDGIP SSSVEMLSRI EQVL PPDPS AVTFFQTKWD IQVQEFYESR GMLEPAAPLD EEIEAILAYH ARKPSELRVR ARHRPDGSTN VGDDPNFWAQ AEPTR MMDG SMLSLWSRRT TLGANGVPIT YRR

Macromolecule #26: ml40

• Macromolecule: Name: ml40 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 33.646066 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MVLPRKLVGQ CCLLLQKKQT AASYMANAGK VGNEEKWAQA AMEYLHEKRH CNDSRKRQHD VDNERRMAFA FDRYCSVNEK IFTERLSRL SDRMTEALET IKQLGMDHAL EEALMLSSEQ PPLNFRRPTL TPPVAGYEPG FGLDVPQLRS RQAEYPPVGR P TDAMEFGE EKDPSFPLVE SFRVEDLTTQ CLNELEERHG EIREAAPTTG VEGEAWEAYV ALQKKALARQ QLIFELCNNG EL RERYDSD VAFRQRVWEE RGMLPLEIER ERLHEEPRHY AQEPAYHPFR KM

Macromolecule #27: ml41

• Macromolecule: Name: ml41 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 22.556877 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLRCSCACRR GVYHNAPSVY PFVKPFHDTP YDQDRGRHDS VGQRYRKNSW PEWMDNGADG TGYGIGLHRT HPLSRLRGNL KRSPSHVPR VLGMMIQGVW HKSGVKLYFR GGKPPNPSVH PYLTGEPCPL YGWKVTDESV IRQFNMPSID GTNFRYKPYV A LQERKIMG VQVPSDSVSL ASGRTTESKP LAKRLFFWR

Macromolecule #28: ml42

• Macromolecule: Name: ml42 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 21.602639 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLRLCRVSLR VQSHQKKRAQ HPNAGTRFGR VYNRGFIRYG FGGFGMSVYT PKKDRRFRVQ PLPSLHANSL ADDTPLVTTT RTLSPNFRA FALQDGGVFF THPSHEQVMR VGQNILAEET KATGMTSMDT YVNSRIQSII AENTVENVAL SHWRRRHMWN L VRTHGKLQ RHWGVSDATK GARSNIYGRP S

Macromolecule #29: ml43

• Macromolecule: Name: ml43 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 31.300406 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MSRNGELCLK KVIISYCPSN GAPNTRQFLA THLPHFHAKY PSVSIDLRPR LWPEMAITGV YRDGSERSYN TKNLSPMGIF LRLNNLVST ANDYDQPFCA SHLHFQRRSV QGTWNPWLWN YETERRRTEA PQWRRKLSEK EWDYYVGQYS AQMKQEEDEI Q RRVADRTC VQEQSTREVQ ERWKRHVVPR MQTDLEFNLS HFKRQHARGQ LQQRPVTMGE YRLFSVPDPR ELGQDAVDTM RR RESHNME VWWRKRKEQL KPP

Macromolecule #30: ml46

• Macromolecule: Name: ml46 / type: protein_or_peptide / ID: 30 / Details: residues 149-159 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 33.984199 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MRPVARLPLS RVLSGCRGFH SQHRTPVLPQ VPAAEVQQAA SESKVRVAYL LHRQPVVKPT PHPLEMEMAF LLQREHQRYS RHESSESAT HFMAQRGQSI DALNRTDPRQ IQSNFFGLEL YQDAMRVVLQ RYKPERRVTP RDLWDPATYG SSNANSNASS P PTRHSLHR KLDDYLHLIV RDEASGKWTV PQTELRGRET LRMAAERAIA TDNGEGLDCY VWSNAPQATV PNANDGSWLF IY VATYLSG RPKFSEFRPK TIDHAWVTRH EMMQYEENFQ SPELVRVLLD ISADSTFES

Macromolecule #31: ml49

• Macromolecule: Name: ml49 / type: protein_or_peptide / ID: 31 / Details: residues 102-109 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 24.501486 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLNRSRLRRV WLGGTAANTS LGRDVMPSTG KYINIVSPTD VAANISAGAM PPEANVAAAL PVPRIQYSKW NRALRSDVYD ELLKLPLRY KLHDFAKLCP PQRCQEEPEA VGVVGRDSAV GYLPPLGPAD PLDTIPFFVH RSSNGVLPGK VFSMHPRNLM P AFYLRIQQ VEGDMFRFEE ELLKIFPTKK IFVRSHSIYV YNVALDGRMV LHHWLLGLGF

Macromolecule #32: ml52

• Macromolecule: Name: ml52 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 29.175984 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MRLHTVRAPI ITRAAMRGYS EARSNYDGTS LPAWPAPGKK PTYPAALSEL RLPQPRMRKT RTEWMYYHGH GGCPGKYGPS REIADFEYA DGTPASISGR RFAFKHHQDH LLVQLIRAAA TVERYDASGL LPRIPGTAEQ RNWDPAIPLF LDDVDEQGRP A PLRTAGDA PGTMVSHVCS RVVDERMGTP THTPNELANR HEGETLEANT MFATNDPSAF VSDTVKLRDD KRPYWSRRRW AL TDKFLVP KSPKPKNTIK DE

Macromolecule #33: ml53

• Macromolecule: Name: ml53 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 35.656383 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLNPPKHYSV ESLRTVGLLP AQLALSRKPR LRPHVGNLKG LVYPLPYYAM WRGNHNKYTY NKSTVCLWGE GDTRSMYHQH YAHAKCPTD YGRGGREFEY LTVKRGKMLQ KPLPRVQYVA EGSKPVWLFK SWHTPLSSPS MWEREVQYAE HTPEHIGAKR P LAVVAPRT MHRYLFLMHM EKVTITVSPL LFGYGHTIQK AVLDFYRRAI SARSPFPKDK VFLFYAIDHI TPRIEVTWLD GT SYVPPVL EGASSQDLIQ MVMEEAWLAA DRMAAEGRVL NPLAIDDYKW DQLVVFKKVR DKEASKGGGR KK

Macromolecule #34: ml63

• Macromolecule: Name: ml63 / type: protein_or_peptide / ID: 34 / Details: residues 64-73 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 18.213924 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLRHCTAHRR YRTAWRELLH PLPVRARKME WLKRDAVEEN EEILRRPYYT IKSYALPPAV GRQESIHNSN NIRGGMHSSH SLDLIMRQP RRVKTPEQLR ALRDRLRFIG VTGPMPQATS VSTKSYTDTY GSRLRPRYPE SWDTVPPHQP SRELL

Macromolecule #35: ml64

• Macromolecule: Name: ml64 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 28.545977 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLRGTRGFLA VSPGVGIAPE TTPVKYTPMM LNIQNMMWWN GKRNLYRATY REKTWYEISR TGAFTKGRRP VMRQKYSREA LQAALAMVP PGFEVADVPR PPQRILAQSE GIVGRWYSNY WTLHSMRYQC LLAGVEWPLG ERQRPRTNYD EPFFFADFEE S KAIRDYRS RWINVNRSLV GMTKRMKEAE EEARYMQFRK LQDTFWSNRK VLVNRVKSMY NQGARTSAKD MPIKTINIKA FL SE

Macromolecule #36: bL12m

• Macromolecule: Name: bL12m / type: protein_or_peptide / ID: 36 / Details: residues built as UNK / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 3.99837 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAA

Macromolecule #37: bL12m

• Macromolecule: Name: bL12m / type: protein_or_peptide / ID: 37 / Details: residues built as UNK / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 2.008194 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

AAAAAAAAAA AAAAAAAAAA AAAAAAAA

Macromolecule #38: bL12m

• Macromolecule: Name: bL12m / type: protein_or_peptide / ID: 38 / Details: residues built as UNK / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 1.937116 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

AAAAAAAAAA AAAAAAAAAA AAAAAAA

Macromolecule #40: ml67

• Macromolecule: Name: ml67 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 93.569273 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLRRFALTSS VALRLRFERD SGHNTVRYRP IPESMQPKHL EDNFTPFPLP KFDESLEYGP VRLRNIPDIE AAKERRRGSR LAATEVLLQ ETLQEENQFA TSGKGDGNMA IAITERHTED VTTPAADSRF PSQTMSPCSH EEEMRGYVVS RDYPLIDRLH C TRSIEELV AQFEDRPQIE SRVAALADMA STVSFRSDEE LLRMFTAISA PFSVDGRGLN FLTVKVSKFG RPYYVPNSLL PA YVNLVDA TTIALVREQP WRLSASPALF IQVLQFMALI KVFEPNKWFT FSDHAPSNRA DYRHAIGVNH STAFWGTGEE LYD FMVELL RVEDDGRIPT MLDLCTREQM VDLLSGFCGV MPCGKAVGDV FKTITDAFLR RVRNDISGPW SAHDWAIVER MYLV TVLCD AGNNEILQLL LSDTASPRGP DFFAAVSRTK DTPTKKRALC LLQEAIDNAS AKADKVTLLG LLESGSEFLL SLVDK GVAH TFATQNLFDY RILNSFLHCS LVADRLRVEQ SVITSLIPSS LRDVQVQMLM SNERNALNPL TSSLPGNSGA IATAPK LKR PLMTMLSQLE YLNSIDSVFI LHSSLMATST DQLVSAVRRL PSGKDSLIVT MSCLRALSVK SLTSPSMKER IACARAL EI VSYELEKGRA VLLPFSEEIL LHDAGAYCDE DLMLWTVAAF LARELPLVKV HTLMHSNCTA RTPYRFLKGG HNLLVSSR S LYDKGAPLLS SLHSKELRLV THNVRLRTPV RDRKCTLQYY NPIRARFVYR RDKPLFDKYH VTARNLAPGF SRGALKHDW RALGVYTPDH PQVPYHPLQT WMLGETTVEA E

Macromolecule #41: ml68

• Macromolecule: Name: ml68 / type: protein_or_peptide / ID: 41 / Details: residues 264-343 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 62.806293 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLYTRRLMTT GGSATADGAV SYSKGSYHIV PKKYTVGKRI AVRSYLDRNR TELSDRTYMP QKAWFEPYTP KKFDMEHQRI SHNFYNLET KLIWTAFDTP ELIGILLHDE TIKGAPHLYD AEFLESAVHW TRESRYWRCI GITKPFYNKT TLRAQCWHDR G LQVGTLVF SQAMRDALMD LERAVRRKEL GLEPNYVWDR WGPVGFIDGA RTDHLPRFAH NPYVDPDGVE VTEVDIAPFN TH EQIKERY GAFIDPDLRP FEGVFRAPSH GALTLDDVPH QEAVRLYRDL MEKADMPVML GNGAEIPPMD MRALFHLSAN PER MKAASE LSSWREVRGM LAPVQEVCDE KVEALRLMEN TRHDAARVRT FYEEKCGFSD FMRTPDKVIT AAVLCYLQEL QRIC TETDW GKPLARCLTD LERVNVMGKD AFLVYRHIED AILDKKRRVW ATRFAGEANE ESTLDYLLEN FGRRTEQTRN VGTTG TEFD REQEPIGRQV QRRVLDSDKA SKLAEVRQKR GKMWSNKKSV FDSLHQKQLQ NVTYGVH

Macromolecule #42: ml69

• Macromolecule: Name: ml69 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 59.010855 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLRRLAGCVA FRKNVVRPFM NSTALLSSPP AAVSAPVIPK SGNEGKDYSV ASGSVDTPVL DQLVGYYVNI QSRKMYYKDF LRIEHQAVR WAFADIRKNS EALAHGLLQT GLRPGQRVLA IQPCNCETFV LQLACAKIGA LLAVVPHHNI SADKLRFYLN E FQPHHLIA REWIVVPEVK QGVLVERNMH FWDMIYNVIP ELGLSYPGQR FQWVHSQEFF FLKKVVITDH NMNLLGVTPM RK MLVWGPF SYYEQRLRRL SALLHPDDPI LALEDPHPAL EDKLHITYSH RNCINAGFLF GNLMGLKAES RFGVMPNHHV DPV GAIVAP YAALTTGAVL VHIHSDMFTD DHAINGIEKL CVEEVVGMLG KKSDFDLLLR HAGNFDADQY EHLKWVVLFE DASE PTVGD EYLQKLAKEL GLEDVFVFRG PLESAYMVMW RSLKRARWEM VPHVEAKVVG DRGTADARIL AANTRGNLKL KGPHI SAGY YNNAGLLTEL VDERGFCSTS REAVMDERGQ LTLHGTQLY

Macromolecule #43: mL70

• Macromolecule: Name: mL70 / type: protein_or_peptide / ID: 43 / Details: residues built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 62.44241 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MCLKRKAPHL FCFCLWSIFL SFRCFCFRSY AIMLPLLSFP TIQISIFLSF KLPITTFLLS PCFVFVFVFA IRYCGELTLN AQLVLFLLY HCAQTQRGPL KEGEMPICPG LCGELAAVPF RVFLGTLPTL AVEERFLRQL QPVFAWYSSR KRVKEQANEF I EIDLASCD AELLLRYSHI YYVRRQLFDE LIERQMTLLD SGKAPKMAEP SLLQCLAGCN MTIADRLQLE IRQLGAAKRA AS VPGRREL DPVARLEVYD YACMMRLVEE DAGAVGDAEM KARAYLPREV IESKLGHLTQ LLLGSDARAA LDKKDVKLLN RMI PPDYTR VGCVEKLRPF DVTAYFRFYG ERINNVKVEN YFKRALWGHV YRRFATTPSF LSGVSTYWAR HSGLDASFTT TTMP QEVAV AVCDQQIQFP AIKFRAQYVY TSPETARQLW RTDAAVPLMR LFPLMGSRTA EDLAAGVLTD AFWMHLGLSE EENLL QDSL LLKVRRFVDE VGDMYETNID SVLKRVDDNF KQVVPQLKAE DLQVDAPLQD GEGEDTVRET VAA

Macromolecule #44: ml71

• Macromolecule: Name: ml71 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 50.313797 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLFRSVSCKN YQRGGWSPGS KHQKHMTLNP TLYLYRFPGP HGPGPYTMKY WWTLGCFPTG MEVPFRLHEF LSTYQQEHVP VEVEEWLRC YIKDPLSELV NASNDFFKAV EVYPEVESAR GYKTLQPSIA PLLVPMKKFE EQLGVKISPV GLRSVLSNPV L KDRFLDDL FDYKSYVEKG GSTPHRRLAR SRFEGSLSVL GECEKCLPEQ HQVEISESLG TFIGATVSPA ETTADDERSL IL LLTTISE GCINAGNYSD AASVLADALM FCHDPDSQAT THANISFASL LNADFKGAEY NGREAALLQP QVKPTSTACA RGY VGWAAA AAYQDDFEKA EAIVKDGLTL YVGNEHLEKL ANKLQALREE QPSVYKQVPR SLRESRSHLP SQQSRGLLSG SGKG FSNEF DWVEFKNKLY PSKMDPRNNE MGSVFRRVGD LGSFISTSRS MERL

Macromolecule #45: ml72

• Macromolecule: Name: ml72 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 49.407 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MSRSAFSSRG ALLFSSAPLQ CQRRWVTRLY TSYYTGVLYP NQLVQPKQRL PADVSVSAIL QKRSEPEAAA ERGSNKGDKV DVSKGEKTP RVSWDDADST DVMRVDNTQQ LKEAPQVERP YVPLGEVAKL ELQGDYYMEG GMFQEALEHY GVVAKAYNYA Y PENHAQRI GIRIKLSAAF RQTGRLESSL ANIEEVLRML DASTRPSLEL ICEALLELGI TREALGMKRE ATEAYEEALE VV NSFHNWG ESHRMLRLLP RLGRRFNYNF EEKFVYFSPF DYDRTFALVD QCLERAETIF NEIGDVEGAI RVLQQRKEMI DKK FFNMRD FAGRIHTMRG HWKRRAQHLT NAPTPDELLR YSPTIHQVHR DFKYELTAPI GREKEVMPGV NRLVLDMGNP YRRR GRLSN KMLKDADHKF ANYVRQKEYN E

Macromolecule #46: ml73

• Macromolecule: Name: ml73 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 41.857875 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MRLARTLHHV ASATTGGGQM LEGLVNDGPE VAHKQHASFT PFSIQPWQAR CVGASRRKLL PQMLMYHGAR LGPRPLIILD HSTKGEAGV AEAARKYESI LSQLSWDYGA VYIPLHAQCT DSSKDLLEQS CQRICAVMDA LDVRWTHFLT YSYGALVAVR M ASSQEFPH RVGTLMSLDT PLVTREFLRN MEQREDIAKA ERDINVPEDG LAFAKQALLS SLEGPLPCPA AEDESLYRDY LF DPNRIFG AGGLVRDESR YVPLKSLLGV RHPVQLIVPS ANPLSDAAAH SEVFGHRRPA VVKCCQRHED LFKESAAKEV AGV LGAWMR RFEPDCFISK RYEQAANEMG QLMLSTAQVS SESAGKGGGE PRKKKEKKKS KA

Macromolecule #47: ml74

• Macromolecule: Name: ml74 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 39.765176 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLVPGLSLTR RAVTSSCCRP LHVVRGFSTT CTLFGLEQLQ DVPTSTSRRP TGLHRGPGKR QTSEREAAQY KFIRRWELQM RDEWDQLEP FKGLPKPKRQ FGNEAAEVIW PYALLLERVV KVHPFTKSIY VYYAQRQSTA RGKLAAEIAR SFAREFLIPI T FHNSQVYT EAEMLLEYSE TPWVVLHSLD NGQKPRILPV APVEGTPAHT AVEQLLAEVV QGCEALGASV ADPVTATRVL NE RPLQNQY VRVDYQWFGD TPDERASHLV RWEFEPEQIE PKIRHRTRHV LDWLNYDGNL PTHRAVHVNA MREKARQKAP RTV AGPRTF YNSAGSRANA RSSRFGGQAA VGK

Macromolecule #48: ml75

• Macromolecule: Name: ml75 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 39.841617 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLRRSDLLLK KGWTHNPGRT RRGGKNLAWR PKMSERTLEQ FVPLHLAFPR RHPNSWQERQ FHLLGYVKWP KEIGFYNAGD NFELTPQAA YRIYKQNCDE TFWTRLHNEK TIIHLLPLVE QDPGTNMVLV DDIFRHHLKR FGADHYIYNA VMQAAAFAKD F PRCEQLLA EMRGLGLEPN AQSYVNMMLG ARLTGKPRDQ AEAFFREGIK TGAISAVMRL DTEFQMWMNQ LERLGSFKAK VG YLSVNEE GASPMPRDMW ALWGWHRTEA KFISRKQMIS EQVQNRVRSG KELVGTVYQK ARRQPWAKYN GMFPYDYNGP ARR PAASFV DAPTPTHNAE VCGTAYA

Macromolecule #49: ml76

• Macromolecule: Name: ml76 / type: protein_or_peptide / ID: 49 / Details: residues 168-176 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 39.638914 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLRLSSWNLK SQHHNVLRRS RPHIHKYREL NRWQRQAQGI SKWDQSHSHR PLPYVERFNP ESVGLTRGTS AFAWKWWHTQ YPWLPNVPP EAAQIDEAQK QERRSHRPPA WDDEFAKVVL NMNDAEIREY LMSKLTDVIF LETQRDGYEL RRLDFEGKPL T SLPEPRII ENFVLEEETI RERVIYQVVE GVFRLSPTSA DRRELRSVAN IIDYVLTHVR AARPTDRERR QERPITSAAL AV MQKCPIQ PQLGFVHALP HDTRDALLQE WERMHHLDWQ FGKAVYTPRS KENVRGNLTW LREDRHYDQR MKFMQEVESG EAR AKHMKL IAEAAGN

Macromolecule #50: Chaperone protein DNAj, putative

• Macromolecule: Name: Chaperone protein DNAj, putative / type: protein_or_peptide / ID: 50 / Details: residues 248-281 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 43.819699 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MYVFRFHISS FHSFLVNFLQ PSRGVGEPSR LLHHLETHPS YMRRVTSTAV HRTVSSVLSP ALLPSFNGTL LLSVCFASCS AAPVRNLYK RLGLDHKATS EEVKAAYRQR ALECHPDVVD DNQKAQAEVD FRAVSEAYDV LIDPQKRKEH DKALGLERTV P PAKKQQEG EGVGCDSKGS FNRGVTRPRN RKPFVRGDAD RNFREAFHGM SLDQVLFRER LRQRRMQKQM EEKAKEGDDG SP AGREESI RRVAAAAAER FAEKVRRQYG PGMLRHARVY TSLSRDPQPP PSDYMPFRPF HGWTVPNGVR TPPEPTLGPT AKV EDVKDV QLAEPAVGDA SHQRKLPKHF PVVQASDGSS LLREETIACM ERERRLPHNM GKLYSYHRPY

Macromolecule #51: ml78

• Macromolecule: Name: ml78 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 35.104535 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MGASAGLIRR GGGVFPDAVS LTLTPSRRVY GGSGRGDLLY ENPDARRHSG RALGVLNGVR HSSQATMPES GQLYYRKLIL HSRPPNGSC AGLQRHCHDT CNWSYLIPSL HRCAESAISA KLWEKMCQLG LEDRSKAWVN LTQYERQRVR DGQNLYRYEV H QRLPLLEE SIGWAQLDDL LGWFRSARRA WVRLPTSVTL SRESSEAGVA SVVSPSSAMS CRLEGHADSR DTTPGRNQVF DT PERVEQL TEATVHRIRE ELQRLNRSER SDCEGSAAMR ASARRLARDE ELSRCVEEEL GWHGVALQHR IPVPK

Macromolecule #52: ml79

• Macromolecule: Name: ml79 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 33.079617 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MRPTFPALGS RAKGYENRVM VYAHRRHRAW YLPPKLAHAR SPLANKSPDE YGNTWDPRTG VEWYHRLRRR GAYRHWPWAR WNDDPVRQH QELSCRRTFS AAVTGANEGV PLWNYYAEVG QEYGLPSHFP LSFMAPFIHQ YTSRAWSRKE IERHLKVVEE R TGLRTIQQ ACDATSELLE WGEEEMGVVP HGLLQHVVML AEDIVLQNKK KAYRKAAHER GILRTTTMER YYALPHLRTG PP MPTTLEQ PSGEFPWGKF STMVGGTRIH PLYRPDGFFK DNMYPA

Macromolecule #53: ml80

• Macromolecule: Name: ml80 / type: protein_or_peptide / ID: 53 / Details: residues 145-174 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 34.883273 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MCCLYTSDVF FWSSLVVSPL PRIVRCVSAP QIRSIPCGSG DFSVMKRSLI ARWQSGVHTP HGVVYRGAKM KNWPEQRIPE NFKFTEEQR FRTKAIPRDV GTIPRNFVLG VLYRHQPCEV GGLWEHCTND PEIVLDSKRH LREVLKQARE EGFVTFERDA I SNEWLCFL TRERYEEVQR IVTAKSEAVD THSGLRGAAA TETSTYAEKF REMNVEAKEA HARRLEEEVA NTTRYLRRFQ QR EIDYLPY TDLNGKVNFM WWYETRDVQQ RADEVLTDSS SSKALAGEEE HKAGSQLEAT TASTS

Macromolecule #54: ml81

• Macromolecule: Name: ml81 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 29.284467 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MKRLFPSAGV SVVLTSSSIV MSCPCNHIFT SRRAYYWPYN EDFVPEGAET SRFQSSGSPG TRRRVLQEYA LSPLFGARVP CCVVGTLRT AKEIIVLKRD IQSLLCELME LPQGSVTLGP LQQLREMMLY RHGTPLSPRL GDERQLNMDA YARRPIAART M MDVFLAED MSLDEIVNFG RLSLGKLQIA LNNLRKENAE KSSADCEDGS AVEPAQLLVD RMGISYCEIP SLDESDYVFA EV GGVAVTD EEAERVAQRW AERCE

Macromolecule #55: ml82

• Macromolecule: Name: ml82 / type: protein_or_peptide / ID: 55 / Details: residues 153-162 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 29.283676 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MRRCAACLTS LPVSSPTGAA VGAPAAPLKT QAPSNRSLLG YPLRRAAAME MLYGGICIQH LAQPPFPLRK IQSESLPPPS LQGERDDLE LEVKDSTGNV MGYRLFPVNI GIRARTESVR VRSEDCYKRF LAQKHCAAAG VPLQFPAPSS ITNSNCLATP R AASHFHPP SSSLSLFTRP ADSQGGDVGR TTPADVAAYH PRAWRPYQML KPMPHNWGPA VRSSGVRGPH MQLLQERIDK KG FGWKRKS RSLWQQDIST AGFRPKRYF

Macromolecule #56: Peptidyl-prolyl cis-trans isomerase

• Macromolecule: Name: Peptidyl-prolyl cis-trans isomerase / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 25.579004 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLHRTRLAQL INGRILQYFV PYERDEVNPV VFMDVTVEGD ALGRVSVELF HDIVPKTTEN FRSLCTGERG YSQCPLFYKG IPFHRVIPG FIVQGGDILL KDGRGNVSVF GFPFPDESFE GKAGKHLRGT VAMAHSAPNQ NGSQFFFNLA RNDHLDGKFV V CGQVIDGW EVVDRVAVLS GSSCGTPVSR AWITECGQSS GDKLEDTERA LSGERALHTM PGKEVLDILS PRY

Macromolecule #57: ml84

• Macromolecule: Name: ml84 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 24.773643 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLRFTRLFRE MAPELQLEYM PVLFTRTILG PQGGFAGEER LVKLEVARKY MEAGHAVTPT EELRRGLWCY NPDTDKYDCF IERNEEFLD FAARKRQWLD VYWRVNTGYL LFGRQSWGQG FLINCPLRKR DVAQKLWEQY KVRIDPRLIE FREKDRRTGI Q ELGHNWCW LYLPGAEELG INREVYDNKR VKVRIHVRKM NSMFALY

Macromolecule #58: ml85

• Macromolecule: Name: ml85 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 22.657951 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MRRLGVFCRG QRNSPLLTRG IATGGRVTNE DRRWWLVHLE CAPDITPGTF IAWLDCCGTH TCKKLIERNI WTIEQVAALD SDQVDELKY REGCLKMDVV WEHARTIITP LRQREVTGGV ESELQGRIME LRKKRELERR REEILKERAN VSEQREETLR K LREAIAAK KAAM(UNK)QKKQA ASEAYGGSSD GGARKEGAEE

Macromolecule #59: ml86

• Macromolecule: Name: ml86 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 22.376191 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MRRCIPARGG FTMKYKKGTG LWDEDHVNDY KTNRYLSARA TMRWYQEMER HQTRNSLNAR RATQSHNNNR GLHHTGRGAF ERELERRGV QVEKYPLTTT TGTMRVAELV ILRRMELEKR AEEALAEQRG ELQKKNPTPS EWYDESKGPL NPNFLRSMRS H YEVDIANL PDTPLIRGQR EFFIGEERGN GAA

Macromolecule #60: ml87

• Macromolecule: Name: ml87 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 22.337521 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLNPTFSLYR KTLQSYPVPP KIRHYDRRWS GSRTNPYNRQ YWRVIMNENY SRPSFWVSDF RHRYLMRTGT DYQGQVPSSP QPGLYQGFS DVHKLLANHP KPQRESRHLP VLPMTPRIVF EHANEKRIDT AKRMRRDRRR IEELKTLEFW GWYMKLQRVR G RWCREQGV SSRGVYGPAV DAAELWG

Macromolecule #61: ml88

• Macromolecule: Name: ml88 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 22.25559 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLQINAFKLV RATPFLLKRT GKPADTPDYK QVYLPYDAAP TERELERERR RFKQAYHGRM EHRKLVEVKE VPLNVYTYGK EGMSLPIAI FKDQKDPVIG PEWTYPGIYE NKIAAQHWYT EELFDKESKE AFESPWQQQI LDNQVKRRMA KVMFRMRQVN M KAVDLFQK ERGSSRRSGG AGEKGKDGGG KK

Macromolecule #62: ml89

• Macromolecule: Name: ml89 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 22.213406 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MSGAFGCGSY RSVVAGTQNV PRRMTFYPSA YELIQLHKAH REVIRHFYVR DKIFDNKFPG NALANGLFKF VPNRRENYHM RELMESIRR RSIWMHRIKQ QREINAKVVE NMEVKYGKKA AASMLCFTTP DSNAYFAPHR YQDVANSWPN YWQHPSVNHV V PKPRWRRH RELGGITRVE DPFAVQASDY

Macromolecule #63: ml90

• Macromolecule: Name: ml90 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 22.123414 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLRSRIPCVV VTLVQCRAMH GKPTQSHKVR TQHSRRWWTQ SKARHLTAMP HDECKSRPHF PAYNEDVDRP MVVPPDAICF NCDKPIDAG DVNSYVWIPA GNATVPTPQG YFFHFGCFKC NRCKYRLGHN KFYSKDGKAW CIPCALGRDV RVPTRRWHTS Y VNTHRTGS RLTGHFFPRH SHQMEFLFDP NS

Macromolecule #64: ml91

• Macromolecule: Name: ml91 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 20.61852 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MLFTRCLLAV TTINSSTASA AGRLIRIRKK SKWIDRRSKR IPHNGKDVWQ FGEQPSCALC HVRFRFKQDY EAHKESELHQ NRLRWVETM KWWEEIGEPH HQQHAASEWE WFRQRVLPAK AAAMGLSEED AARELRRAVM HETPRWYSRI QPPNARSEIK E PRDQRWPS SPKW

Macromolecule #65: Peptidyl-prolyl cis-trans isomerase

• Macromolecule: Name: Peptidyl-prolyl cis-trans isomerase / type: protein_or_peptide / ID: 65 / Details: residues 46-64 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 20.542291 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MRISERAFMD IAIGTKAPRR IVFKLFPRKC PSAVKNFIEL CSGNVSTDTY ESGNRDKLIS ESALPQLTYK NSTFHRVEKG YLIQGGDIV TGRGTEQLSI YGGTFSAPEE VRASVFDKPG LVGTASSSPN AHGSQFFILT AKEANHLNGT CICFGQVADG L DVVQEIEQ VPIDPSGFPS LKVSIVDCGV LE

Macromolecule #66: ml93

• Macromolecule: Name: ml93 / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 18.929691 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MFRVTGLQLK NPVVFKQGQG MFSHQLKRLL QKKSIHRYNW DPLPMYDPRK LVHASRHMDV ETWREVPDPH WDERSYLVPD QMFYNIPVP PEYKDAYWWR ELQARRVQCP VEWVSHRMYN KGDRQRYDFQ DLAFRKKFEF SYEEVVKNAK DMRS

Macromolecule #67: ml94

• Macromolecule: Name: ml94 / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 17.830959 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MAQWVPKTAW KVSNLNKRYG PGYITSGFSS ENTLGYTHMV HDSHKLREKL SSRKGIFVID VRDPGERKLN PVPRSVALHH HDLLSGASC PILPQQKSAA ELFVLASNES RGLNSAAALR RWGYDSVVQV DYNTLVEAGY VSRCDYATAG TKDSAEQAVS G ED

Macromolecule #68: ml95

• Macromolecule: Name: ml95 / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 17.803377 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MFRPTTAIAD SFKEHYHRVH LPRRLALQRY ARQQSLRNAA KGNVKAEEVP YKYNRWWVNE EHEFVHQYAF VEDPEVTKAK RETLPPVTR ENIWKEPQQT FFLPFAPYVR VVDYPKDPDA KFLKPVNIPR WKDYMQRTKP VIPRTWY

Macromolecule #69: ml96

• Macromolecule: Name: ml96 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 16.387654 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MNDIYARRLA QATMFHQLMR CHGTLWAATQ VTKEQMDYNF IREEFMRVNG RRAMPLLLGA AANENLHQLH LSHLSEHCAW GESARALAV QRQTPLSQRV AALGRMAETI HQVKTASTVQ NLFNEQISCM EGISSFEEEP LIEGE

Macromolecule #70: ml97

• Macromolecule: Name: ml97 / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 12.974785 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MSSRFFQKYF IRCGNCQTIQ RYAKGYKPIP NPILFDSDAH CRSYHRERRD CTGLTGTLVT CRCDKCARVH SHWTVMDFQE FLDAKLVMT PEERTALLWP GAGSRAEPSS GTSN

Macromolecule #71: ml98

• Macromolecule: Name: ml98 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 12.968644 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MVLRGVRLRS VAVSCYGSSL TAATRCLSVR TEDFFSKEAI SHARRVSWAP HTTEKKQGAF AKLARSNFGD PLPSSFAQEP YFEEEIEAH RKHHRPDVYI YKYNVSPTHF SLRE

Macromolecule #72: ml99

• Macromolecule: Name: ml99 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 12.334969 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MYQRTRFLWS SWRDYPLGSR DRRGRFNMDE AAAALQLNPA YAAALYRPLN YTFHIRGQLY PAQKGRPSRP GSLAASQGRM FPLYQRNDR LDKELFRLNS RGLTTE

Macromolecule #73: ml100

• Macromolecule: Name: ml100 / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 10.600362 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

MALFSCFRCG YMYEFAVSNS YCRKLTLRND HCPRCDQLTL FRFMSVSGMV GNMPFKPIGV PGPSYATLWW RKTREGKEAS APLDAVCKS DRW

Macromolecule #74: UNK

• Macromolecule: Name: UNK / type: protein_or_peptide / ID: 74 / Details: unassigned residues built as UNK / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 3.932839 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #75: UNK

• Macromolecule: Name: UNK / type: protein_or_peptide / ID: 75 / Details: unassigned residues built as UNK / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 3.422209 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

Macromolecule #76: UNK

• Macromolecule: Name: UNK / type: protein_or_peptide / ID: 76 / Details: unassigned residues built as UNK / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 1.039273 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

Macromolecule #77: UNK

• Macromolecule: Name: UNK / type: protein_or_peptide / ID: 77 / Details: unassigned residues built as UNK / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 15.081565 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

Macromolecule #78: UNK

• Macromolecule: Name: UNK / type: protein_or_peptide / ID: 78 / Details: unassigned residues built as UNK / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 1.890321 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

Macromolecule #79: UNK

• Macromolecule: Name: UNK / type: protein_or_peptide / ID: 79 / Details: unassigned residues built as UNK / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 2.060531 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #80: UNK

• Macromolecule: Name: UNK / type: protein_or_peptide / ID: 80 / Details: unassigned residues built as UNK / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 1.464797 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

Macromolecule #81: UNK

• Macromolecule: Name: UNK / type: protein_or_peptide / ID: 81 / Details: unassigned residues built as UNK / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 869.063 Da
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #82: UNK

• Macromolecule: Name: UNK / type: protein_or_peptide / ID: 82 / Details: unassigned residues built as UNK / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 1.294587 KDa
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #83: UNK

• Macromolecule: Name: UNK / type: protein_or_peptide / ID: 83 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 528.644 Da
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #84: UNK

• Macromolecule: Name: UNK / type: protein_or_peptide / ID: 84 / Details: unassigned residues built as UNK / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 954.168 Da
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

Macromolecule #85: UNK

• Macromolecule: Name: UNK / type: protein_or_peptide / ID: 85 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 698.854 Da
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #86: UNK

• Macromolecule: Name: UNK / type: protein_or_peptide / ID: 86 / Details: unassigned residues built as UNK / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 613.749 Da
• Recombinant expression: Organism: Trypanosoma brucei brucei (eukaryote)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #39: 12S rRNA

• Macromolecule: Name: 12S rRNA / type: rna / ID: 39 / Number of copies: 1
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Theoretical: 376.7485 KDa

Sequence

String:

AUUUUACCAA UUAAGAAGAA UAUUAUAAUA AUGGGUGUCU UAUAUUUUAA AUAAAUAUUU AAAUUCCGUG UAGUAAAUUU AUUAUUUGU AUUAUUUAUA UAAUAGGUGU AUUAUAUUUA AAUUUUAAAU UUGUUGUUUU AUAUUUAGAU ACAUAUUUAU A GAUUAAUA UAUUUAAAUA AUAUUUUAAA AUUUAUUGAA CUGUAAUUAU UAGUUUAAUA UUUUUAGUUU GAUGUUGAAA UA UUUAAUU AAAGAUGUUA CAGUUGUUCU AUAUGUACCA AAUAAAUAUA GUAAGAUUAU UUUAGUUGAA UUAAUAAAUA AAU AUUUAU UUUUCUUUGU AAAUAUUAUG AACAAUUUAA AAAUUAAUCU GUUUAACUAA AAUGUUAUAU AUAAUAAUCU AAGU UAAUU UGAAUAUUAA AAGUACAAGU AUAAUUUGUA AUUCUAAAGU AUAUUAAUGG UAUAUUUUUA GUAGGUAAAU GAAAA GUAU AAAUGGAUAU AACUUAAUAU UUAAUAUUUG UUUAAUGAAA AGUAUUUUAU UAUUAUAUUG UAUAGUAUUA UUAUAG UGU AUAGUUUUUU AAAAAUAUAA AAAUAUUGUU AAUAAAAUUA UCGUAUUUUA AGUGCGUUAA UUAAAUGCGU UUAUCUA AG AUAAUUAUUU AAGAUUAUUC UUGUAAAUAU AUUUAAAUAU UAAUAAUUCU UAAAAUAAAG AAACAUCCUC AAUUGCAA U AUUAUUGUAG CAUAGUAAUU UCUUAACUAA GUAUUUAAUU UUUCCAUAGA AAAUUUUUAA AUUACAAGAA AGAAAAUAA AGUAUGAAUU AAUAUCAAAA UUUUAAUAAA AAUUAAAAAA UUAAAAUAGG GCAAGUCCUA CUCUCCUUUA CAAAAGAAAC AUUAUGAUA UGUAAUUGUA UGUUUGAUUG GGGCAAUACU AUAUUUAUUU AUAUAGCAUA AGAACUAUAU UCUUUGAAAU U AUAAAAGG UUCGAGCAGG UUAACAAGCA UUAAAAAUAA AUGUGUUUCA UCGUCUACUU AUUACCAUGA UUGAUUGUUC AU CAAAAUA GUAAUUCGUU AGUUGGGUUA AAAUCGUUGU AAAGCAGAUU UGUUUAUAUA UUUAAUUUUU AUAAUUAAUA AUA AUUAAU AUAAGUACGC AAGGAUUGAU UAUUGAAAAA AGAAAGAAGA AUAUAAUUUA UA

Macromolecule #87: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 87 / Number of copies: 6 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #88: NICOTINAMIDE-ADENINE-DINUCLEOTIDE

• Macromolecule: Name: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / type: ligand / ID: 88 / Number of copies: 1 / Formula: NAD
• Molecular weight: Theoretical: 663.425 Da

Chemical component information

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM / Nicotinamide adenine dinucleotide

Macromolecule #89: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 89 / Number of copies: 7 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Vitrification: Cryogen name: ETHANE-PROPANE / Chamber humidity: 98 % / Chamber temperature: 278 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
• Image recording: Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Average electron dose: 40.0 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Software - Name: CTFFIND
• Startup model: Type of model: OTHER / Details: initial model from 2D class averages in EMAN
• Initial angle assignment: Type: OTHER
• Final 3D classification: Software - Name: RELION
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION
• Final reconstruction: Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.39 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 31619

Atomic model buiding 1

• Details: We used Coot and O ofr initial model building and refined the structure using PHENIX
• Refinement: Protocol: AB INITIO MODEL / Overall B value: 52.2

Output model

PDB-6hix:
Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the large mitoribosomal subunit