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- SASDHC2: PieE-FAD: 2,4-dichlorophenol 6-monooxygenase bound to flavin aden... -

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Basic information

Entry
Database: SASBDB / ID: SASDHC2
SamplePieE-FAD: 2,4-dichlorophenol 6-monooxygenase bound to flavin adenine dinucleotide
  • 2,4-dichlorophenol 6-monooxygenase (protein), PieE, Streptomyces sp. SCSIO 03032
  • Flavin adenine dinucleotide (other), FAD
Function / homologyAromatic-ring hydroxylase, C-terminal / FAD-binding domain / FAD binding domain / FAD binding / monooxygenase activity / FAD/NAD(P)-binding domain superfamily / 2,4-dichlorophenol 6-monooxygenase
Function and homology information
Biological speciesStreptomyces sp. SCSIO 03032 (bacteria)
Contact author
  • Normand Cyr (Département de biochimie, Université de Montréal, Montréal, Canada)

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Structure visualization

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Models

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Sample

SampleName: PieE-FAD: 2,4-dichlorophenol 6-monooxygenase bound to flavin adenine dinucleotide
Specimen concentration: 17 mg/ml / Entity id: 1962 / 1963
BufferName: 20 mM Tris, 150 mM NaCl, 5 mM DTT, 2% glycerol / pH: 7.5
Entity #1962Name: PieE / Type: protein / Description: 2,4-dichlorophenol 6-monooxygenase / Formula weight: 66.552 / Num. of mol.: 6 / Source: Streptomyces sp. SCSIO 03032 / References: UniProt: W0C4C9
Sequence: MGSSHHHHHH SSGLVPRGSH MTGSEGTAPD IRVPVLIVGG GPAGLTAALA LSRYGVPHLL VNRHHGTAHT PRAHLLNQRT GEIFRDLGIA DRVEAHATPG HLMANHVFMS TFAGPEVARI GAYGNGPDRI GEYRAASPSG LCNLPQHLLE PLLVEAVQEA CVGQLRFGHE ...Sequence:
MGSSHHHHHH SSGLVPRGSH MTGSEGTAPD IRVPVLIVGG GPAGLTAALA LSRYGVPHLL VNRHHGTAHT PRAHLLNQRT GEIFRDLGIA DRVEAHATPG HLMANHVFMS TFAGPEVARI GAYGNGPDRI GEYRAASPSG LCNLPQHLLE PLLVEAVQEA CVGQLRFGHE FVSLEQDEHG VTSRITDRRT GRDYTVRSDY LIGADGARSR VLAQLGIALD GATGIARAVT TWFEADLSRY SAHRPALLYM GAVPGSPPAD GRVFVSLRPW TEWLHLTFPP PTADVDVEDH EAVRAGIRES IGDPTVDVTI KNVSAWEVNS AVAPRYASGR VFCVGDAVHQ NPPTNGLGLN SAVADSFNLC WKLKLALEGL AGPGLLDTYH DERQPVGRQI VDRAFRSMVD LIGIPQALGF TEGQSPEEQW RLLDTLHEDT EEARQRRAAL AAATAAIHGQ ANAHGVELGY RYRTGALVPD GTPEPADERD PELYYRATTW PGARLPHAWL ENGRHRCSTL DVTGRGRFTL LTGPGGEPWR DAARDAALDT GVEVAVLPIG AGGGPRDPYG TWAELREVEE SGAVLVRPDG HVAWRARDHG HAKELPEVMA RVLHQPDPAA RRTGGPGA
Entity #1963Name: FAD / Type: other / Description: Flavin adenine dinucleotide / Formula weight: 0.786 / Num. of mol.: 6
Sequence:
C27H33N9O1 5P2

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Experimental information

BeamInstrument name: Université de Montréal Xenocs BioXolver L with MetalJet
City: Montréal / 国: Canada / Type of source: X-ray in house / Wavelength: 0.134 Å / Dist. spec. to detc.: 0.6 mm
DetectorName: Pilatus3 R 300K / Type: pixel counting / Pixsize x: 172 mm
Scan
Title: PieE-FAD: 2,4-dichlorophenol 6-monooxygenase bound to flavin adenine dinucleotide
Measurement date: Oct 22, 2019 / Storage temperature: 24 °C / Cell temperature: 20 °C / Exposure time: 30 sec. / Number of frames: 190 / Unit: 1/A /
MinMax
Q0.0107 0.3003
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 215 /
MinMax
Q0.0133653 0.298921
P(R) point1 215
R0 132
Result
Type of curve: sec /
ExperimentalPorod
MW390 kDa390 kDa
Volume-624 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.1042 0.0003 0.1043 0.0005
Radius of gyration, Rg4.73 nm0.01 4.81 nm0.04

MinMax
D-13.2
Guinier point2 12

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