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- SASDHB2: Soluble guanylate cyclase (nitric oxide, NO, bound) -

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Basic information

Entry
Database: SASBDB / ID: SASDHB2
SampleSoluble guanylate cyclase (nitric oxide, NO, bound)
  • Soluble guanylyl cyclase alpha-1 subunit (protein), Manduca sexta
  • Soluble guanylyl cyclase beta-1 subunit (protein), Manduca sexta
Function / homology
Function and homology information


guanylate cyclase / guanylate cyclase activity / intracellular signal transduction / heme binding / GTP binding / cytoplasm
Similarity search - Function
Haem NO binding associated domain superfamily / Haem NO binding associated / Heme NO binding associated / Heme NO-binding / H-NOX domain superfamily / Haem-NO-binding / Adenylyl cyclase class-4/guanylyl cyclase, conserved site / Guanylate cyclase signature. / NO signalling/Golgi transport ligand-binding domain superfamily / Adenylyl- / guanylyl cyclase, catalytic domain ...Haem NO binding associated domain superfamily / Haem NO binding associated / Heme NO binding associated / Heme NO-binding / H-NOX domain superfamily / Haem-NO-binding / Adenylyl cyclase class-4/guanylyl cyclase, conserved site / Guanylate cyclase signature. / NO signalling/Golgi transport ligand-binding domain superfamily / Adenylyl- / guanylyl cyclase, catalytic domain / Adenylyl cyclase class-3/4/guanylyl cyclase / Adenylate and Guanylate cyclase catalytic domain / Guanylate cyclase domain profile. / Nucleotide cyclase
Similarity search - Domain/homology
guanylate cyclase / Guanylate cyclase soluble subunit beta-1
Similarity search - Component
Biological speciesManduca sexta (tobacco hornworm)
Contact author
  • Daniel Rosenberg (Lawrence Berkeley National laboratory, Berkeley, CA, USA)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #3917
Type: atomic / Chi-square value: 2.28674286997
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Soluble guanylate cyclase (nitric oxide, NO, bound) / Specimen concentration: 7 mg/ml / Entity id: 1956 / 1957
BufferName: 50 mM KH2PO4,150 mM NaCl, 2% glycerol, 500 µM NO, / pH: 7.4
Entity #1956Type: protein / Description: Soluble guanylyl cyclase alpha-1 subunit / Formula weight: 78.49 / Num. of mol.: 1 / Source: Manduca sexta / References: UniProt: O77105
Sequence: MTCPFRRASS QHQFANGGSS APKKPEFRSR TSSVHLTGPE EEDGERNTLT LKHMSEALQL LTAPSNECLH AAVTSLTKNQ SDHYHKYNCL RRLPDDVKTC RNYAYLQEIY DAVRATDSVN TKDFMAKLGE YLILTAFSHN CRLERAFKCL GTNLTEFLTT LDSVHDVLHD ...Sequence:
MTCPFRRASS QHQFANGGSS APKKPEFRSR TSSVHLTGPE EEDGERNTLT LKHMSEALQL LTAPSNECLH AAVTSLTKNQ SDHYHKYNCL RRLPDDVKTC RNYAYLQEIY DAVRATDSVN TKDFMAKLGE YLILTAFSHN CRLERAFKCL GTNLTEFLTT LDSVHDVLHD QDTPLKDETM EYEANFVCTT SQEGKIQLHL TTESEPVAYL LVGSLKAIAK RLYDTQTDIR LRSYTNDPRR FRYEINAVPL HQKSKEDSCE LVNEAASVAT STKVTDLKIG VASFCKAFPW HFITDKRLEL VQLGAGFMRL FGTHLATHGS SLGTYFRLLR PRGVPLDFRE ILKRVNTPFM FCLKMPGSTA LAEGLEIKGQ MVFCAESDSL LFVGSPFLDG LEGLTGRGLF ISDIPLHDAT RDVILVGEQA RAQDGLRRRM DKLKNSIEEA SKAVDKEREK NVSLLHLIFP PHIAKRLWLG EKIEAKSHDD VTMLFSDIVG FTSICATATP MMVIAMLEDL YSVFDIFCEE LDVYKVETIG DAYCVASGLH RKVETHAPQI AWMALRMVET CAQHLTHEGN PIKMRIGLHT GTVLAGVVGK TMLKYCLFGH NVTLANKFES GSEPLKINVS PTTYEWLIKF PGFDMEPRDR SCLPNSFPKD IHGTCYFLHK YTHPGTDPGE PQVKHIREAL KDYGIGQANS TDVDTEEPT
Entity #1957Type: protein / Description: Soluble guanylyl cyclase beta-1 subunit / Formula weight: 68.098 / Num. of mol.: 1 / Source: Manduca sexta / References: UniProt: O77106
Sequence: MYGFVNYALE LLVMKTFDEE TWETIKKKAD VAMEGSFLVR QIYEDEITYN LITAAVEVLQ IPADAILELF GKTFFEFCQD SGYDKILQVL GATPRDFLQN LDGLHDHLGT LYPGMRSPSF RCTERPEDGA LVLHYYSDRP GLEHIVIGIV KTVASKLHNT EVKVEILKTK ...Sequence:
MYGFVNYALE LLVMKTFDEE TWETIKKKAD VAMEGSFLVR QIYEDEITYN LITAAVEVLQ IPADAILELF GKTFFEFCQD SGYDKILQVL GATPRDFLQN LDGLHDHLGT LYPGMRSPSF RCTERPEDGA LVLHYYSDRP GLEHIVIGIV KTVASKLHNT EVKVEILKTK EECDHVQFLI TETSTTGRVS APEIAEIETL SLEPKVSPAT FCRVFPFHLM FDRDLNIVQA GRTVSRLLPR VTRPGCKITD VLDTVRPHLE MTFANVLAHI NTVYVLKTKP EEMSVTDPHE EIASLRLKGQ MLYIPETDVV VFQCYPSVTN LDDLTRRGLC IADIPLHDAT RDLVLMSEQF EADYKLTQNL EVLTDKLQQT FRELELEKQK TDRLLYSVLP ISVATELRHR RPVPARRYDT VTLLFSGIVG FANYCARNSD HKGAMKIVRM LNDLYTAFDV LTDPKRNPNV YKVETVGDKY MAVSGLPEYE VAHAKHISLL ALDMMDLSQT VTVDGEPVGI TIGIHSGEVV TGVIGHRMPR YCLFGNTVNL TSRCETTGVP GTINVSEDTY NYLMREDNHD EQFELTYRGH VTMKGKAEPM QTWFLTRKIH

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Experimental information

BeamInstrument name: Advanced Light Source (ALS) 12.3.1 (SIBYLS)
City: Berkeley, CA / 国: USA / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.1127 Å / Dist. spec. to detc.: 2.1 mm
DetectorName: Pilatus3 X 2M / Pixsize x: 172 mm
Scan
Title: Soluble guanylate cyclase (nitric oxide, NO, bound) / Measurement date: Dec 3, 2018 / Storage temperature: 4 °C / Cell temperature: 20 °C / Exposure time: 3 sec. / Number of frames: 20 / Unit: 1/A /
MinMax
Q0.0142 0.3804
Distance distribution function P(R)
Sofotware P(R): GNOM 4.6 / Number of points: 329 /
MinMax
Q0.01505 0.3804
P(R) point1 329
R0 142
Result
Type of curve: sec /
ExperimentalPorod
MW130.7 kDa136 kDa
Volume-218 nm3

P(R)GuinierGuinier error
Forward scattering, I0147.4 145.01 0.7
Radius of gyration, Rg4.5 nm4.37 nm0.02

MinMax
D-14.2
Guinier point1 29

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