SASDGW5: Suppressor of Fused from Drosophila (Suppressor of fused homolog,...

Basic information

• Entry: Database: SASBDB / ID: SASDGW5

Sample

Suppressor of Fused from Drosophila

• Suppressor of fused homolog (protein), dSuFu, Drosophila melanogaster

Function / homology

Function:

cytoplasmic sequestering of transcription factor / negative regulation of nucleocytoplasmic transport / Hedgehog 'off' state / Degradation of GLI1 by the proteasome / GLI3 is processed to GLI3R by the proteasome / Hedgehog 'on' state / nuclear localization sequence binding / transcription factor binding / negative regulation of smoothened signaling pathway / protein sequestering activity / negative regulation of DNA-binding transcription factor activity / protein-containing complex / nucleus / cytosol / cytoplasm

Domain/homology:

Suppressor of fused / Suppressor of fused, eukaryotic / Suppressor of fused C-terminal / Suppressor of fused, N-terminal / Suppressor of fused, C-terminal domain superfamily / Suppressor of Fused Gli/Ci N terminal binding domain / Suppressor of fused-like domain / Suppressor of fused protein (SUFU)

Component:

Suppressor of fused homolog

• Biological species: Drosophila melanogaster (fruit fly)

Contact author

• Valerie Biou (IBPC-CNRS)

Models

• Model #3798: ; Type: atomic / Symmetry: P1; Comment: Model built with Dadimodo using 2 domains as rigid bodies and a 5-residue flexible linker.; Chi-square value: 3.415104
• Model #3799: ; Type: atomic / Symmetry: P1; Comment: Model built with Dadimodo using 2 domains as rigid bodies and a 5-residue flexible linker.; Chi-square value: 3.090564

Sample

• Sample: Name: Suppressor of Fused from Drosophila / Specimen concentration: 10 mg/ml
• Buffer: Name: 50 mM bis-TRIS pH 5.5, 200 mM NaCl, 10% glycerol / pH: 5.5

Entity #1903

Name: dSuFu / Type: protein / Description: Suppressor of fused homolog / Formula weight: 52.787 / Num. of mol.: 1 / Source: Drosophila melanogaster / References: UniProt: q9vg38
Sequence:

MAEANLDKKP EVKPPPGLKA IIDHLGQVYP NQPNPLQVTT LLKYWLGGQD PLDYISMYNY PGDVDRNVPP HWHYISFGLS DLHGDERVHL REEGVTRSGM GFELTFRLAK TEIELKQQIE NPEKPQRPPT WPANLLQAIG RYCFQTGNGL CFGDNIPWRK SLDGSTTSKL QNLLVAQDPQ LGCIDTPTGT VDFCQIVGVF DDELEQASRW NGRGVLNFLR QDMQTGGDWL VTNMDRQMSV FELFPETLLN LQDDLEKQGS DLAGVNADFT FRELKPTKEV KEEVDFQALS EKCANDENNR QLTDTQMKRE EPSFPQSMSM SSNSLHKSCP LDFQAQAPNC ISLDGIEITL APGVAKYLLL AIKDRIRHGR HFTFKAQHLA LTLVAESVTG SAVTVNEPYG VLGYWIQVLI PDELVPRLME DFRSAGLDEK CEPKERLELE WPDKNLKLII DQPEPVLPMS LDAAPLKM

Experimental information

• Beam: Instrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 1.804 mm
• Detector: Name: AVIEX PCCD170170 / Type: CCD

Scan

Title: Suppressor of Fused from Drosophila / Measurement date: May 7, 2012 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Number of frames: 160 / Unit: 1/A /

	Min	Max
Q	0.0057	0.621

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 1091 /

	Min	Max
Q	0.00566257	0.620449
P(R) point	1	1091
R	0	88

Result

Type of curve: sec /

	Experimental	Porod
MW	51 kDa	-
Volume	-	11.7 nm3

	P(R)	P(R) error	Guinier	Guinier error
Forward scattering, I0	1.745	0.000546	1.74	0.0008437
Radius of gyration, Rg	2.73 nm	0.012	2.72 nm	0.002

	Min	Max	Error
D	-	8.8	0.2
Guinier point	23	76	-