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- SASDFY3: Semi-synthetic nucleosome core particle (NCP) in complex with lys... -

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Entry
Database: SASBDB / ID: SASDFY3
SampleSemi-synthetic nucleosome core particle (NCP) in complex with lysine-specific demethylase (LSD2) and the dehydrogenase domain plus linker of cytokine-like nuclear factor (NPAC delta-205)
  • Lysyne-specific Demethylase LSD2 (protein), LSD2, Homo sapiens
  • NPAC linker+DH (delta-205) (protein), NPAC delta205, Homo sapiens
  • Histone H3 (protein), H3, Xenopus laevis
  • Histone H4 (protein), H4, Xenopus laevis
  • Histone H2a (protein), H2a, Xenopus laevis
  • Histone H2b (protein), H2b, Xenopus laevis
  • 147bp 601 Widom sequence (DNA), 601-147bp, synthetic construct
Biological speciesHomo sapiens (human)
Xenopus laevis (African clawed frog)
synthetic construct (others)

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Sample

SampleName: Semi-synthetic nucleosome core particle (NCP) in complex with lysine-specific demethylase (LSD2) and the dehydrogenase domain plus linker of cytokine-like nuclear factor (NPAC delta-205)
Specimen concentration: 1.8 mg/ml / Entity id: 1516 / 1519 / 1520 / 1521 / 1522 / 1523 / 1524
BufferName: 15 mM HEPES, 200 mM NaCl / pH: 7.3
Entity #1516Name: LSD2 / Type: protein / Description: Lysyne-specific Demethylase LSD2 / Formula weight: 88.832 / Num. of mol.: 1 / Source: Homo sapiens
Sequence: KKKATETTDE DEDGGSEKKY RKCEKAGCTA TCPVCFASAS ERCAKNGYTS RWYHLSCGEH FCNECFDHYY RSHKDGYDKY TTWKKIWTSN GKTEPSPKAF MADQQLPYWV QCTKPECRKW RQLTKEIQLT PQIAKTYRCG MKPNTAIKPE TSDHCSLPED LRVLEVSNHW ...Sequence:
KKKATETTDE DEDGGSEKKY RKCEKAGCTA TCPVCFASAS ERCAKNGYTS RWYHLSCGEH FCNECFDHYY RSHKDGYDKY TTWKKIWTSN GKTEPSPKAF MADQQLPYWV QCTKPECRKW RQLTKEIQLT PQIAKTYRCG MKPNTAIKPE TSDHCSLPED LRVLEVSNHW WYSMLILPPL LKDSVAAPLL SAYYPDCVGM SPSCTSTNRA AATGNASPGK LEHSKAALSV HVPGMNRYFQ PFYQPNECGK ALCVRPDVME LDELYEFPEY SRDPTMYLAL RNLILALWYT NCKEALTPQK CIPHIIVRGL VRIRCVQEVE RILYFMTRKG LINTGVLSVG ADQYLLPKDY HNKSVIIIGA GPAGLAAARQ LHNFGIKVTV LEAKDRIGGR VWDDKSFKGV TVGRGAQIVN GCINNPVALM CEQLGISMHK FGERCDLIQE GGRITDPTID KRMDFHFNAL LDVVSEWRKD KTQLQDVPLG EKIEEIYKAF IKESGIQFSE LEGQVLQFHL SNLEYACGSN LHQVSARSWD HNEFFAQFAG DHTLLTPGYS VIIEKLAEGL DIQLKSPVQC IDYSGDEVQV TTTDGTGYSA QKVLVTVPLA LLQKGAIQFN PPLSEKKMKA INSLGAGIIE KIALQFPYRF WDSKVQGADF FGHVPPSASK RGLFAVFYDM DPQKKHSVLM SVIAGEAVAS VRTLDDKQVL QQCMATLREL FKEQEVPDPT KYFVTRWSTD PWIQMAYSFV KTGGSGEAYD IIAEDIQGTV FFAGEATNRH FPQTVTGAYL SGVREASKIA AF
Entity #1519Name: NPAC delta205 / Type: protein / Description: NPAC linker+DH (delta-205) / Formula weight: 37.438 / Num. of mol.: 4 / Source: Homo sapiens
Sequence: TASEPVKDAD PHFHHFLLSQ TEKPAVCYQA ITKKLKICEE ETGSTSIQAA DSTAVNGSIT PTDKKIGFLG LGLMGSGIVS NLLKMGHTVT VWNRTAEKCD LFIQEGARLG RTPAEVVSTC DITFACVSDP KAAKDLVLGP SGVLQGIRPG KCYVDMSTVD ADTVTELAQV ...Sequence:
TASEPVKDAD PHFHHFLLSQ TEKPAVCYQA ITKKLKICEE ETGSTSIQAA DSTAVNGSIT PTDKKIGFLG LGLMGSGIVS NLLKMGHTVT VWNRTAEKCD LFIQEGARLG RTPAEVVSTC DITFACVSDP KAAKDLVLGP SGVLQGIRPG KCYVDMSTVD ADTVTELAQV IVSRGGRFLE APVSGNQQLS NDGMLVILAA GDRGLYEDCS SCFQAMGKTS FFLGEVGNAA KMMLIVNMVQ GSFMATIAEG LTLAQVTGQS QQTLLDILNQ GQLASIFLDQ KCQNILQGNF KPDFYLKYIQ KDLRLAIALG DAVNHPTPMA AAANEVYKRA KALDQSDNDM SAVYRAYIH
Entity #1520Name: H3 / Type: protein / Description: Histone H3 / Formula weight: 15.271 / Num. of mol.: 2 / Source: Xenopus laevis
Sequence:
ARTKQTARKS TGGKAPRKQL ATKAARKSAP ATGGVKKPHR YRPGTVALRE IRRYQKSTEL LIRKLPFQRL VREIAQDFKT DLRFQSSAVM ALQEASEAYL VALFEDTNLC AIHAKRVTIM PKDIQLARRI RGERA
Entity #1521Name: H4 / Type: protein / Description: Histone H4 / Formula weight: 11.236 / Num. of mol.: 2 / Source: Xenopus laevis
Sequence:
SGRGKGGKGL GKGGAKRHRK VLRDNIQGIT KPAIRRLARR GGVKRISGLI YEETRGVLKV FLENVIRDAV TYTEHAKRKT VTAMDVVYAL KRQGRTLYGF GG
Entity #1522Name: H2a / Type: protein / Description: Histone H2a / Formula weight: 13.95 / Num. of mol.: 2 / Source: Xenopus laevis
Sequence:
SGRGKQGGKT RAKAKTRSSR AGLQFPVGRV HRLLRKGNYA ERVGAGAPVY LAAVLEYLTA EILELAGNAA RDNKKTRIIP RHLQLAVRND EELNKLLGRV TIAQGGVLPN IQSVLLPKKT ESSKSAKSK
Entity #1523Name: H2b / Type: protein / Description: Histone H2b / Formula weight: 13.494 / Num. of mol.: 2 / Source: Xenopus laevis
Sequence:
AKSAPAPKKG SKKAVTKTQK KDGKKRRKTR KESYAIYVYK VLKQVHPDTG ISSKAMSIMN SFVNDVFERI AGEASRLAHY NKRSTITSRE IQTAVRLLLP GELAKHAVSE GTKAVTKYTS AK
Entity #1524Name: 601-147bp / Type: DNA / Description: 147bp 601 Widom sequence / Formula weight: 45.202 / Num. of mol.: 1 / Source: synthetic construct
Sequence:
ATCGAGAATC CCGGTGCCGA GGCCGCTCAA TTGGTCGTAG ACAGCTCTAG CACCGCTTAA ACGCACGTAC GCGCTGTCCC CCGCGTTTTA ACCGCCAAGG GGATTACTCC CTAGTCTCCA GGCACGTGTC AGATATATAC ATCCGAT

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Experimental information

BeamInstrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 2.87 mm
DetectorName: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm
ScanTitle: Semi-synthetic nucleosome core particle (NCP) in complex with lysine-specific demethylase (LSD2) and the dehydrogenase domain plus linker of cytokine-like nuclear factor (NPAC delta-205)
Measurement date: Dec 11, 2017 / Storage temperature: 4 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 70 / Unit: 1/nm /
MinMax
Q0.0808 2.8618
ResultType of curve: sec /
ExperimentalPorod
MW530 kDa-
Volume-1100 nm3

P(R)GuinierGuinier error
Forward scattering, I0162.2 157.8 0.2
Radius of gyration, Rg8.396 nm7.84 nm0.01

MinMax
D-30.35
Guinier point2 25

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