SASDFA6: Proteolytic fragment of phage flagella binding tail protein YSD1_...

Basic information

• Entry: Database: SASBDB / ID: SASDFA6

Sample

Proteolytic fragment of phage flagella binding tail protein YSD1_29 (amino acids 373-1296)

• Flagella binding tail protein (protein), YSD1_29, Salmonella virus Chi

• Function / homology: Tip attachment protein J / Putative phage tail protein / Minor tail protein
• Biological species: Salmonella virus Chi

Contact author

• Grinter Grinter (Monash University, Melbourne, Australia)

Models

• Model #2864: ; Type: dummy / Radius of dummy atoms: 5.00 A / Chi-square value: 0.504 / P-value: 0.000020; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: Proteolytic fragment of phage flagella binding tail protein YSD1_29 (amino acids 373-1296); Specimen concentration: 10 mg/ml
• Buffer: Name: 20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % glycerol / pH: 7.8

Entity #1532

Name: YSD1_29 / Type: protein / Description: Flagella binding tail protein / Formula weight: 102.637 / Num. of mol.: 1 / Source: Salmonella virus Chi / References: UniProt: M9NVD3_9CA
Sequence:

IRVGTVEDGT LPDGKIKVVA LQDQFAFQLN TFNQVEPPAG YEPNLTPAIA RRIVYEMPYV DLVQQLPDGE LNAVGPNDAF INSQAEKPTD MSAAYDMGIM AEGESGFDVR GNGDFGAFGS LASDIDYLST QIVLSEMTDM WDDVQPGFVA RIAKPVLDGQ RTQMEIAEEF VRIDAINGNV ITVARGVLDT IPFRHQKSEM LWVTTYDGGT DWQKYAGNES IDIKILPWTL GGGRFPIEDA PIDHLDMDFR QIRPYPPGNV QHYLASTSTL QRWYVPSALT YTANAGETPD TYTLTWAHRD RVLQGDKPVP HIDGDIGPEP GTTYTIRVYN QEGTLVRTES GINGTTWNWP YATAAADMNV EASLFDPVLA TLRLTSVRNG RESWEYYEMK VSVYKKPPQF VYDASLMQFA AQPYNATIDP NPPTPPMDGP YVSALMQFAA QPDGSEDFGP AESMNGPNVA LLPHQVTQTS SIITPLDTLL YETPYIQLSR DGRDLNTSKV SAYVARSSDR TVDSYTLFTK HETDANYTSA GTQPWTPWGM STVGLGFFTD EVTIDPTSDK DGVPVAPAKV GDLLLIDQEL VSITAINGRT YKIGRGVADT IPAQHYSSRP VWLISVGFGY SDMAFGDNEK AMVIIRPDTY GTDIPLSKLY PLQLQMQYRP KRPYPPGLMM IGGQPFFNTA SGLADDFNPY DNLKAKDVVC TYAHRNRVSQ STTARDHFAV GINPEPGVKY RVRIGYAYWS QSGSGFSLLS QFETEDAGFI MRAADLERWG RQAGYAQDAG GYSTLNVTVN AIRDGMLNWQ GYTMTVRVPS FPLPPGQKPG GGDGPWNPPG GGNTGGTTPP TEPPDRPDPS EPGTTDPSEP TKPDEPEEPQ PPVDPEDPDT DPPEPPEPPV DPTNVPGWSL SWDHGWAMTL PDFRYVPPAD EEPELEHHHH HH

Experimental information

• Beam: Instrument name: Australian Synchrotron SAXS/WAXS / City: Melbourne / 国: Australia / Shape: Point / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.10322 Å / Dist. spec. to detc.: 1.426 mm
• Detector: Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm

Scan

Title: Proteolytic fragment of phage flagella binding tail protein YSD1_29 (amino acids 373-1296)
Measurement date: Apr 4, 2017 / Storage temperature: 4 °C / Cell temperature: 23 °C / Exposure time: 1 sec. / Number of frames: 10 / Unit: 1/A /

	Min	Max
Q	0.0057	0.3169

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 350 /

	Min	Max
Q	0.006435	0.142516
P(R) point	1	350
R	0	273.8

Result

Type of curve: sec /

	Experimental	Porod
MW	94.26 kDa	97 kDa
Volume	-	155 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	0.279	0.27	-
Radius of gyration, Rg	6.13 nm	5.61 nm	0.06

	Min	Max
D	-	27.38
Guinier point	3	42