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- SASDEX6: Cold-active AMS8 lipase without polyhistidine affinity tag -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDEX6
SampleCold-active AMS8 lipase without polyhistidine affinity tag
  • LipAMS8 (protein), AMS8, Pseudomonas sp. AMS8
Function / homology
Function and homology information


triacylglycerol lipase / triglyceride lipase activity / calcium ion binding
Similarity search - Function
Hemolysin-type calcium-binding conserved site / Hemolysin-type calcium-binding region signature. / RTX calcium-binding nonapeptide repeat / RTX calcium-binding nonapeptide repeat (4 copies) / Serralysin-like metalloprotease, C-terminal / Alpha/Beta hydrolase fold
Similarity search - Domain/homology
Biological speciesPseudomonas sp. AMS8 (bacteria)
Contact author
  • Norhayati Yaacob (Synchrotron Light Research Institue)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2567
Type: dummy / Software: (r10552) / Radius of dummy atoms: 2.50 A / Symmetry: P1 / Chi-square value: 1.921 / P-value: 0.033735

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Sample

SampleName: Cold-active AMS8 lipase without polyhistidine affinity tag
Specimen concentration: 2 mg/ml
BufferName: 50 mM Tris HCl, 5 mM CaCl2 / pH: 8
Entity #1054Name: AMS8 / Type: protein / Description: LipAMS8 / Formula weight: 50.019 / Num. of mol.: 1 / Source: Pseudomonas sp. AMS8 / References: UniProt: E1B2U7
Sequence: MGVFDYKNLG TEGSKALFAD AMAITLYSYH NLDNGFAVGY QHNGFGLGLP ATLVGALLGS TDSQGVIPGI PWNPDSEKAA LEAVNKAGWT PISASTLGYG GKVDARGTFF GEKAGYTTAQ VEVLGKYDGD GKLLEIGIGF RGTSGPRETL ITDSIGDLVS DLLAALGPKD ...Sequence:
MGVFDYKNLG TEGSKALFAD AMAITLYSYH NLDNGFAVGY QHNGFGLGLP ATLVGALLGS TDSQGVIPGI PWNPDSEKAA LEAVNKAGWT PISASTLGYG GKVDARGTFF GEKAGYTTAQ VEVLGKYDGD GKLLEIGIGF RGTSGPRETL ITDSIGDLVS DLLAALGPKD YAKNYAGEAF GTLLKDVAAY AGSHGLTGKD VVVSGHSLGG LAVNSMADLS GNKWSGFYKD SNYVAYASPT QSSGDKVLNI GYENDPVFRA LDGSSFNFSS LGVHDKPHES TTDNIVSFND HYASTLWNVL PFSIVNVPTW LSHLPTGYGD GLTRVLDSKF YDLTSRDSTI IVANLSDPAR ANTWVQDLNR NAEPHKGNTF IIGSDGNDLI QGGKGVDFIE GGKGNDTIRD NSGHNTFLFG GQFGQDRVIG YQSTDKLVFK DVEGSADWRD HAKVVGGDTV LSFGADSVTL VGVGLAGVGG DGISIS

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Experimental information

BeamInstrument name: Synchrotron Light Research Institute (SLRI) BL1.3W
City: Nakhon Ratchasima / 国: Thailand / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.138 Å / Dist. spec. to detc.: 1.7 mm
DetectorName: MAR 345 Image Plate
Scan
Title: AMS8 lipase without polyhistidine affinity tag / Measurement date: Mar 6, 2018 / Storage temperature: 10 °C / Cell temperature: 25 °C / Exposure time: 600 sec. / Number of frames: 1 / Unit: 1/A /
MinMax
Q0.0214 0.3742
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1137 /
MinMax
Q0.0311753 0.273381
P(R) point1 1137
R0 98
Result
Type of curve: single_conc
ExperimentalStandardPorod
MW50 kDa40.1 kDa54 kDa
Volume--86.8 nm3

P(R)GuinierGuinier error
Forward scattering, I081.58 77.71 0.52
Radius of gyration, Rg3.04 nm2.859 nm0.021

MinMax
D-9.8
Guinier point37 115

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