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- SASDDD9: Cardiac myosin binding protein C: tri-helix bundle from the motif... -
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Open data
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Basic information
Entry | Database: SASBDB / ID: SASDDD9 |
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![]() | Cardiac myosin binding protein C: tri-helix bundle from the motif with C2 domain
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Function / homology | ![]() C zone / regulation of muscle filament sliding / striated muscle myosin thick filament / cardiac myofibril / regulation of striated muscle contraction / positive regulation of ATP-dependent activity / Striated Muscle Contraction / A band / structural constituent of muscle / ventricular cardiac muscle tissue morphogenesis ...C zone / regulation of muscle filament sliding / striated muscle myosin thick filament / cardiac myofibril / regulation of striated muscle contraction / positive regulation of ATP-dependent activity / Striated Muscle Contraction / A band / structural constituent of muscle / ventricular cardiac muscle tissue morphogenesis / sarcomere organization / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function |
Biological species | human sequence obtained using E. coli expression |
![]() | ![]() Title: A Highly Conserved Yet Flexible Linker Is Part of a Polymorphic Protein-Binding Domain in Myosin-Binding Protein C. Authors: Katharine A Michie / Ann H Kwan / Chang-Shung Tung / J Mitchell Guss / Jill Trewhella / ![]() ![]() Abstract: The nuclear magnetic resonance (NMR) structure of the tri-helix bundle (THB) of the m-domain plus C2 (ΔmC2) of myosin-binding protein C (MyBP-C) has revealed a highly flexible seven-residue linker ...The nuclear magnetic resonance (NMR) structure of the tri-helix bundle (THB) of the m-domain plus C2 (ΔmC2) of myosin-binding protein C (MyBP-C) has revealed a highly flexible seven-residue linker between the structured THB and C2. Bioinformatics shows significant patterns of conservation across the THB-linker sequence, with the linker containing a strictly conserved serine in all MyBP-C isoforms. Clinically linked mutations further support the functional significance of the THB-linker region. NMR, small-angle X-ray scattering, and binding studies show the THB-linker plus the first ten residues of C2 undergo dramatic changes when ΔmC2 binds Ca-calmodulin, with the linker and C2 N-terminal residues contributing significantly to the affinity. Modeling of all available experimental data indicates that the THB tertiary structure must be disrupted to form the complex. These results are discussed in the context of the THB-linker and the N-terminal residues of C2 forming a polymorphic binding domain that could accommodate multiple binding partners in the dynamic sarcomere. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
-Data source
SASBDB page | ![]() |
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-Related structure data
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External links
Related items in Molecule of the Month |
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-Models
Model #2155 | ![]() Type: atomic / Comment: one state of 2-state MultiFoXS model, weight 0.753 / Chi-square value: 3.56671154143 / P-value: 0.820000 ![]() |
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Model #2156 | ![]() Type: atomic / Comment: one state of 2-state MultiFoXS model, weight 0.247 / Chi-square value: 3.56671154143 / P-value: 0.820000 ![]() |
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Sample
![]() | Name: Cardiac myosin binding protein C: tri-helix bundle from the motif with C2 domain Specimen concentration: 0.98-4.89 |
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Buffer | Name: 150 mM NaCl, 10 mM MES, 2 mM TCEP, 1 mM NaN3 at 4°C / pH: 6.5 |
Entity #1178 | Name: tri-helix bundle-C2 / Type: protein Description: cardiac myosin binding protein C: tri-helix bundle-C2 Formula weight: 15.474 / Num. of mol.: 1 / Source: human sequence obtained using E. coli expression / References: UniProt: Q14896 Sequence: GPGSEDVWEI LRQAPPSEYE RIAFQYGVTD LRGMLKRLKG MRRDEKKSTA FQKKLEPAYQ VSKGHKIRLT VELADHDAEV KWLKNGQEIQ MSGSKYIFES IGAKRTLTIS QCSLADDAAY QCVVGGEKCS TELFVKE |
-Experimental information
Beam | Instrument name: Australian Synchrotron SAXS/WAXS / City: Melbourne / 国: Australia ![]() ![]() ![]() | ||||||||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 1M / Pixsize x: 172 mm | ||||||||||||||||||||||||||||||||||||
Scan | Measurement date: Apr 18, 2015 / Cell temperature: 22 °C / Exposure time: 1 sec. / Number of frames: 26 / Unit: 1/A /
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Distance distribution function P(R) |
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Result | Comments: The concentration series SAXS data and EOM models are provided in the full entry zip archive.
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