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- SASDD65: Ubiquitinating/deubiquitinating enzyme SdeA 207 914 -

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Basic information

Entry
Database: SASBDB / ID: SASDD65
SampleUbiquitinating/deubiquitinating enzyme SdeA 207 914
  • Ubiquitinating/deubiquitinating enzyme SdeA (protein), SdeA, Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)
Function / homology
Function and homology information


NAD+-protein-arginine ADP-ribosyltransferase / deNEDDylase activity / NAD+-protein-arginine ADP-ribosyltransferase activity / protein deneddylation / Transferases; Acyltransferases; Aminoacyltransferases / K63-linked deubiquitinase activity / protein deubiquitination / cysteine-type peptidase activity / host cell / nucleotidyltransferase activity ...NAD+-protein-arginine ADP-ribosyltransferase / deNEDDylase activity / NAD+-protein-arginine ADP-ribosyltransferase activity / protein deneddylation / Transferases; Acyltransferases; Aminoacyltransferases / K63-linked deubiquitinase activity / protein deubiquitination / cysteine-type peptidase activity / host cell / nucleotidyltransferase activity / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / protein ubiquitination / nucleotide binding / proteolysis / extracellular region
Similarity search - Function
SidE, DUB domain / SidE, mono-ADP-ribosyltransferase domain / SidE mono-ADP-ribosyltransferase domain / SidE DUB domain / SidE, PDE domain / SidE phosphodiesterase (PDE) domain
Similarity search - Domain/homology
Ubiquitinating/deubiquitinating enzyme SdeA
Similarity search - Component
Biological speciesLegionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) (bacteria)
CitationDate: 2018 May 23
Title: Insights into catalysis and function of phosphoribosyl-linked serine ubiquitination
Authors: Kalayil S / Bhogaraju S / Bonn F / Shin D / Liu Y / Gan N / Basquin J / Grumati P / Luo Z
Contact author
  • Donghyuk Shin (IBCII, Goethe University-Medical Faculty)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #1883
Type: dummy / Radius of dummy atoms: 3.25 A / Chi-square value: 1.053 / P-value: 0.547735
Search similar-shape structures of this assembly by Omokage search (details)
Model #1884
Type: atomic / Chi-square value: 1.840
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Ubiquitinating/deubiquitinating enzyme SdeA 207 914 / Specimen concentration: 2.64 mg/ml
BufferName: 10 mM HEPES 150 mM NaCl 1 mM TCEP / pH: 7.5
Entity #1013Name: SdeA / Type: protein / Description: Ubiquitinating/deubiquitinating enzyme SdeA / Formula weight: 71.811 / Num. of mol.: 1
Source: Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)
References: UniProt: Q5ZTK4
Sequence: HGLSHTLRTM AYAELIVEEA RKAKLRGETL GKFKDGRTIA DVTPQELKKI MIAQAFFVAG RDDEASDAKN YQKYHEQSRD AFLKYVKDNE STLIPDVFKD QEDVNFYARV IEDKSHDWES TPAHVLINQG HMVDLVRVKQ PPESFLQRYF SSMQRWIGSQ ATEAVFGIQR ...Sequence:
HGLSHTLRTM AYAELIVEEA RKAKLRGETL GKFKDGRTIA DVTPQELKKI MIAQAFFVAG RDDEASDAKN YQKYHEQSRD AFLKYVKDNE STLIPDVFKD QEDVNFYARV IEDKSHDWES TPAHVLINQG HMVDLVRVKQ PPESFLQRYF SSMQRWIGSQ ATEAVFGIQR QFFHATYEVV AGFDSDNKEP HLVVSGLGRY VIGEDGQPIR EAPKKGQKEG DLKVFPQTYK LKENERLMRV DEFLKLPEIQ NTFPGSGKHL QGGMPGMNEM DYWNRLNSLN RARCENDVDF CLKQLQTAHD KAKIEPIKQA FQSSKGKERR QPNVDEIAAA RIIQQILANP DCIHDDHVLI NGQKLEQQFF RDLLAKCEMA VVGSLLNDTD IGNIDTLMRH EKDTEFHSTN PEAVPVKIGE YWINDQRINN SSGNITQKKH DLIFLMQNDA WYFSRVNAIA QNRDKGSTFK EVLITTLMTP LTSKALVDTS QAKPPTRLFR GLNLSEEFTK GLIDQANAMI ANTTERLFTD HSPEAFKQIK LNDLSKMSGR TNASTTTEIK LVKETWDSNV IFEMLDPDGL LHSKQVGRHG EGTESEFSVY LPEDVALVPV KVTLDGKTQK GENRYVFTFV AVKSPDFTPR H

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Experimental information

BeamInstrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3 mm
DetectorName: Pilatus 2M
Scan
Title: Ubiquitinating/deubiquitinating enzyme SdeA 207 914 / Measurement date: Nov 26, 2017 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 0.045 sec. / Number of frames: 20 / Unit: 1/nm /
MinMax
Q0.0614 3.8928
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 813 /
MinMax
Q0.072479 2.31877
P(R) point1 813
R0 11.2
Result
Type of curve: single_conc
ExperimentalStandardPorod
MW73 kDa80 kDa80 kDa
Volume--128 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.05686 7.55E-5 0.057 7.3E-5
Radius of gyration, Rg3.475 nm0.006 3.45 nm0.03

MinMax
D-11.2
Guinier point5 111

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