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- SASDCW4: Bovine β Cardiac Myosin S1 fragment (Myosin-7) MgADP.VO4+OM -

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ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDCW4
SampleBovine β Cardiac Myosin S1 fragment (Myosin-7) MgADP.VO4+OM
  • Bovine β Cardiac Myosin S1 fragment (protein), BCS1 (myosin-7), Bos taurus
Function / homology
Function and homology information


muscle filament sliding / myosin filament / myosin II complex / adult heart development / sarcomere organization / microfilament motor activity / myofibril / cardiac muscle contraction / sarcomere / actin filament binding ...muscle filament sliding / myosin filament / myosin II complex / adult heart development / sarcomere organization / microfilament motor activity / myofibril / cardiac muscle contraction / sarcomere / actin filament binding / calmodulin binding / ATP binding / cytoplasm
Similarity search - Function
DNA repair protein XRCC4-like, C-terminal / Myosin tail / Myosin tail / Myosin N-terminal SH3-like domain / Myosin S1 fragment, N-terminal / Myosin, N-terminal, SH3-like / Myosin N-terminal SH3-like domain profile. / Myosin head, motor domain / Myosin head (motor domain) / Myosin motor domain profile. ...DNA repair protein XRCC4-like, C-terminal / Myosin tail / Myosin tail / Myosin N-terminal SH3-like domain / Myosin S1 fragment, N-terminal / Myosin, N-terminal, SH3-like / Myosin N-terminal SH3-like domain profile. / Myosin head, motor domain / Myosin head (motor domain) / Myosin motor domain profile. / Myosin. Large ATPases. / IQ motif profile. / IQ motif, EF-hand binding site / Kinesin motor domain superfamily / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Biological speciesBos taurus (cattle)
CitationDate: 2017 Dec
Title: Mechanistic and structural basis for activation of cardiac myosin force production by omecamtiv mecarbil
Authors: Planelles-Herrero V / Hartman J / Robert-Paganin J / Malik F
Contact author
  • Vicente José Planelles Herrero (Institut Curie)

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Structure visualization

Downloads & links

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Models

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Sample

SampleName: Bovine β Cardiac Myosin S1 fragment (Myosin-7) MgADP.VO4+OM
Specimen concentration: 4 mg/ml
BufferName: 10 mM HEPES, 50 mM NaCl, 1 mM NaN3, 2.5 mM MgCl2, 2 mM ADP, 1 mM TCEP
pH: 7.5
Entity #621Name: BCS1 (myosin-7) / Type: protein / Description: Bovine β Cardiac Myosin S1 fragment / Formula weight: 116.582 / Num. of mol.: 1 / Source: Bos taurus / References: UniProt: Q9BE39
Sequence: MVDAEMAAFG EAAPYLRKSE KERLEAQTRP FDLKKDVFVP DDKEEFVKAT ILSREGGKVT AETEHGKTVT VKEDQVLQQN PPKFDKIEDM AMLTFLHEPA VLYNLKERYA SWMIYTYSGL FCVTINPYKW LPVYNAEVVA AYRGKKRSEA PPHIFSISDN AYQYMLTDRE ...Sequence:
MVDAEMAAFG EAAPYLRKSE KERLEAQTRP FDLKKDVFVP DDKEEFVKAT ILSREGGKVT AETEHGKTVT VKEDQVLQQN PPKFDKIEDM AMLTFLHEPA VLYNLKERYA SWMIYTYSGL FCVTINPYKW LPVYNAEVVA AYRGKKRSEA PPHIFSISDN AYQYMLTDRE NQSILITGES GAGKTVNTKR VIQYFAVIAA IGDRSKKEQA TGKGTLEDQI IQANPALEAF GNAKTVRNDN SSRFGKFIRI HFGATGKLAS ADIETYLLEK SRVIFQLKAE RDYHIFYQIL SNKKPELLDM LLITNNPYDY AFISQGETTV ASIDDAEELM ATDNAFDVLG FTTEEKNSMY KLTGAIMHFG NMKFKLKQRE EQAEPDGTEE ADKSAYLMGL NSADLLKGLC HPRVKVGNEY VTKGQNVQQV VYAKGALAKA VYERMFNWMV TRINATLETK QPRQYFIGVL DIAGFEIFDF NSFEQLCINF TNEKLQQFFN HHMFVLEQEE YKKEGIEWEF IDFGMDLQAC IDLIEKPMGI MSILEEECMF PKATDMTFKA KLFDNHLGKS SNFQKPRNIK GKPEAHFSLI HYAGTVDYNI IGWLQKNKDP LNETVVDLYK KSSLKMLSSL FANYAGFDTP IEKGKGKAKK GSSFQTVSAL HRENLNKLMT NLRSTHPHFV RCIIPNETKS PGVIDNPLVM HQLRCNGVLE GIRICRKGFP NRILYGDFRQ RYRILNPAAI PEGQFIDSRK GAEKLLGSLD IDHNQYKFGH TKVFFKAGLL GLLEEMRDER LSRIITRIQA QSRGVLSRME FKKLLERRDS LLIIQWNIRA FMGVKNWPMA PKKPDPKKDE AKAGAKAAAA PAPAPAPPPA PEPSKEPEFD PSKIKIEFTP EQIEEFKEAF TLFDRTPKCE MKITYGQCGD VLRALGQNPT QAEVLRVLGK PKQEELNSKM MDFDTFLPML QHISKNKDTG TYEDFVEGLR VFDKEGNGTV MGAELRHVLA TLGEKLTEDE VEKLMAGQED SNGCINYEAF VKHIMAG

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Experimental information

BeamInstrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.10332 Å / Dist. spec. to detc.: 1.786 mm
DetectorName: AVIEX / Type: CCD
Scan
Title: Bovine β Cardiac Myosin S1 fragment (Myosin-7) MgADP.VO4+OM
Measurement date: Mar 1, 2016 / Storage temperature: 4 °C / Exposure time: 0.5 sec. / Number of frames: 41 / Unit: 1/A /
MinMax
Q0.0199 0.614
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 264 /
MinMax
Q0.0199 0.222
P(R) point1 264
R0 126.4
Result
Type of curve: single_conc / Comments: BATCH mode
ExperimentalStandardPorod
MW112.91 kDa112.91 kDa100 kDa
Volume--162 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.1495 0.0006 0.15 4.8E-5
Radius of gyration, Rg3.68 nm0.002 3.6 nm0.03

MinMax
D-12.6
Guinier point1 29

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