SASDBL2: MBP-PICK1 fusion (Maltose Binding Protein fused to Protein Intera...

基本情報

• 登録情報: データベース: SASBDB / ID: SASDBL2

試料

MBP-PICK1 fusion

• Maltose Binding Protein fused to Protein Interacting with C kinase 1 (protein), MBP-PICK1, Homo sapiens

機能・相同性

分子機能:

membrane curvature sensor activity / postsynaptic early endosome / glial cell development / neuronal ion channel clustering / cellular response to decreased oxygen levels / Arp2/3 complex binding / Trafficking of GluR2-containing AMPA receptors / regulation of Arp2/3 complex-mediated actin nucleation / epigenetic programming of gene expression / negative regulation of Arp2/3 complex-mediated actin nucleation / ゲノム刷り込み / monoamine transport / protein kinase C-activating G protein-coupled receptor signaling pathway / retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum / dendritic spine organization / long-term synaptic depression / dendritic spine maintenance / receptor clustering / regulation of insulin secretion / positive regulation of receptor internalization / cellular response to glucose starvation / trans-Golgi network membrane / G protein-coupled receptor binding / protein kinase C binding / Cell surface interactions at the vascular wall / intracellular protein transport / phospholipid binding / endocytic vesicle membrane / actin filament binding / シナプス小胞 / presynaptic membrane / postsynaptic density / 細胞骨格 / neuron projection / protein domain specific binding / protein phosphorylation / signaling receptor binding / シナプス / perinuclear region of cytoplasm / ゴルジ体 / identical protein binding / metal ion binding / 細胞膜 / 細胞質基質 / 細胞質

ドメイン・相同性:

PICK1, BAR domain / Arfaptin homology (AH) domain / Arfaptin family / Arfaptin-like domain / Arfaptin homology (AH) domain profile. / Arfaptin-like domain / AH/BAR domain superfamily / PDZドメイン / PDZ domain profile. / Domain present in PSD-95, Dlg, and ZO-1/2. / PDZドメイン / PDZ superfamily

構成要素:

PRKCA-binding protein

• 生物種: Homo sapiens (ヒト)
• 引用: ジャーナル: Mol Biol Cell / 年: 2015; タイトル: PICK1 is implicated in organelle motility in an Arp2/3 complex-independent manner.; 著者: Yadaiah Madasu / Changsong Yang / Malgorzata Boczkowska / Kelley A Bethoney / Adam Zwolak / Grzegorz Rebowski / Tatyana Svitkina / Roberto Dominguez / ; 要旨: PICK1 is a modular scaffold implicated in synaptic receptor trafficking. It features a PDZ domain, a BAR domain, and an acidic C-terminal tail (ACT). Analysis by small- angle x-ray scattering suggests a structural model that places the receptor-binding site of the PDZ domain and membrane-binding surfaces of the BAR and PDZ domains adjacent to each other on the concave side of the banana-shaped PICK1 dimer. In the model, the ACT of one subunit of the dimer interacts with the PDZ and BAR domains of the other subunit, possibly accounting for autoinhibition. Consistently, full-length PICK1 shows diffuse cytoplasmic localization, but it clusters on vesicle-like structures that colocalize with the trans-Golgi network marker TGN38 upon deletion of either the ACT or PDZ domain. This localization is driven by the BAR domain. Live-cell imaging further reveals that PICK1-associated vesicles undergo fast, nondirectional motility in an F-actin-dependent manner, but deleting the ACT dramatically reduces vesicle speed. Thus the ACT links PICK1-associated vesicles to a motility factor, likely myosin, but, contrary to previous reports, PICK1 neither binds nor inhibits Arp2/3 complex.

登録者

• Roberto Dominguez (Penn, University of Pennsylvania, Philadelphia, PA, United States)

モデル

• モデル #402: ; タイプ: dummy / ソフトウェア: crysol / ダミー原子の半径: 1.90 A / カイ2乗値: 1.1881; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: MBP-PICK1 fusion / 試料濃度: 0.46-7.50
• バッファ: 名称: 50 mM Tris / pH: 7.5 / 組成: 300 mM NaCl, 1 mM maltose, 1 mM EGTA, 2 mM DTT

要素 #266

名称: MBP-PICK1 / タイプ: protein
記述: Maltose Binding Protein fused to Protein Interacting with C kinase 1
分子量: 87.089 / 分子数: 2 / 由来: Homo sapiens / 参照: UniProt: Q9NRD5
配列:

MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDA ALAAAQTNAA AMFADLDYDI EEDKLGIPTV PGKVTLQKDA QNLIGISIGG GAQYCPCLYI VQVFDNTPAA LDGTVAAGDE ITGVNGRSIK GKTKVEVAKM IQEVKGEVTI HYNKLQADPK QGMSLDIVLK KVKHRLVENM SSGTADALGL SRAILCNDGL VKRLEELERT AELYKGMTEH TKNLLRAFYE LSQTHRAFGD VFSVIGVREP QPAASEAFVK FADAHRSIEK FGIRLLKTIK PMLTDLNTYL NKAIPDTRLT IKKYLDVKFE YLSYCLKVKE MDDEEYSCIA LGEPLYRVST GNYEYRLILR CRQEARARFS QMRKDVLEKM ELLDQKHVQD IVFQLQRLVS TMSKYYNDCY AVLRDADVFP IEVDLAHTTL AYGLNQEEFT DGEEEEEEED TAAGEPSRDT RGAAGPLDKG GSWCDS

実験情報

• ビーム: 設備名称: Cornell High Energy Synchrotron Source (CHESS) G1; 地域: Ithaca, NY / 国: USA / 線源: X-ray synchrotronシンクロトロン
• 検出器: 名称: Finger Lakes CCD / タイプ: CCD

スキャン

タイトル: MPP-PICK1 fusion at 3.75mg/ml / 測定日: 2015年10月13日 / セル温度: 4 °C / 照射時間: 40 sec. / フレーム数: 20 / 単位: 1/nm /

	Min	Max
Q	0.1034	2.9352

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 151 /

	Min	Max
Q	0.00790906	0.0991546
P(R) point	1	151
R	0	276

結果

D max: 27.6 / カーブのタイプ: single_conc
コメント: This dataset was collected at a concentration of 3.75 mg/ml.

	実験値	Porod
分子量	172.2 kDa	-
体積	-	483 nm3

	P(R)	Guinier
前方散乱 I0	41.58	41.42
慣性半径, Rg	8.7 nm	8.38 nm