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- SASDAU4: uPAR H47C/N259C (Urokinase plasminogen activator surface receptor... -
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Open data
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Basic information
Entry | Database: SASBDB / ID: SASDAU4 |
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![]() | uPAR H47C/N259C
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function |
Biological species | ![]() ![]() |
![]() | ![]() Title: A flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR). Authors: Haydyn D T Mertens / Magnus Kjaergaard / Simon Mysling / Henrik Gårdsvoll / Thomas J D Jørgensen / Dmitri I Svergun / Michael Ploug / ![]() Abstract: The urokinase-type plasminogen activator receptor (uPAR) provides a rendezvous between proteolytic degradation of the extracellular matrix and integrin-mediated adhesion to vitronectin. These ...The urokinase-type plasminogen activator receptor (uPAR) provides a rendezvous between proteolytic degradation of the extracellular matrix and integrin-mediated adhesion to vitronectin. These processes are, however, tightly linked because the high affinity binding of urokinase regulates the binding of uPAR to matrix-embedded vitronectin. Although crystal structures exist to define the corresponding static bi- and trimolecular receptor complexes, it is evident that the dynamic property of uPAR plays a decisive role in its function. In the present study, we combine small angle x-ray scattering, hydrogen-deuterium exchange, and surface plasmon resonance to develop a structural model describing the allosteric regulation of uPAR. We show that the flexibility of its N-terminal domain provides the key for understanding this allosteric mechanism. Importantly, our model has direct implications for understanding uPAR-assisted cell adhesion and migration as well as for translational research, including targeted intervention therapy and non-invasive tumor imaging in vivo. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
-Data source
SASBDB page | ![]() |
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-Related structure data
Related structure data | C: citing same article ( |
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Similar structure data |
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External links
Related items in Molecule of the Month |
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-Models
Model #78 | ![]() Type: dummy / Software: dammif / Radius of dummy atoms: 1.90 A / Symmetry ![]() ![]() |
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Model #80 | ![]() Type: mix / Software: coral / Radius of dummy atoms: 1.90 A / Symmetry ![]() ![]() |
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Sample
![]() | Name: uPAR H47C/N259C / Sample MW: 36.98 kDa / Specimen concentration: 1.50-4.40 / Concentration method: A280 |
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Buffer | Name: Sodium Phosphate / Concentration: 25.00 mM / pH: 7.2 / Composition: Glycerol 5.000 %, NaSO4 50.000 mM |
Entity #66 | Name: uPAR![]() Description: Urokinase plasminogen activator surface receptor ![]() Formula weight: 36.98 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: Q03405 Sequence: MGHPPLLPLL LLLHTCVPAS WGLRCMQCKT NGDCRVEECA LGQDLCRTTI VRLWEEGEEL ELVEKSCTHS EKTNRTLSYR TGLKITSLTE VVCGLDLCNQ GNSGRAVTYS RSRYLECISC GSSDMSCERG RHQSLQCRSP EEQCLDVVTH WIQEGEEGRP KDDRHLRGCG ...Sequence: MGHPPLLPLL LLLHTCVPAS WGLRCMQCKT NGDCRVEECA LGQDLCRTTI VRLWEEGEEL ELVEKSCTHS EKTNRTLSYR TGLKITSLTE VVCGLDLCNQ GNSGRAVTYS RSRYLECISC GSSDMSCERG RHQSLQCRSP EEQCLDVVTH WIQEGEEGRP KDDRHLRGCG YLPGCPGSNG FHNNDTFHFL KCCNTTKCNE GPILELENLP QNGRQCYSCK GNSTHGCSSE ETFLIDCRGP MNQCLVATGT HEPKNQSYMV RGCATASMCQ HAHLGDAFSM NHIDVSCCTK SGCNHPDLDV QYRSGAAPQP GPAHLSLTIT LLMTARLWGG TLLWT |
-Experimental information
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Detector | Name: Pilatus 1M-W / Pixsize x: 0.172 mm | ||||||||||||||||||||||||||||||
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Distance distribution function P(R) |
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Result |
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