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- SASDA97: PlaB -

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Basic information

Entry
Database: SASBDB / ID: SASDA97
SamplePlaB
  • PlaB (protein), Legionella pneumophila
Biological speciesLegionella pneumophila (bacteria)
CitationJournal: Sci Rep / Year: 2015
Title: Automated pipeline for purification, biophysical and x-ray analysis of biomacromolecular solutions.
Authors: Melissa A Graewert / Daniel Franke / Cy M Jeffries / Clement E Blanchet / Darja Ruskule / Katja Kuhle / Antje Flieger / Bernd Schäfer / Bernd Tartsch / Rob Meijers / Dmitri I Svergun /
Abstract: Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed ...Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed an all-in-one system combining in-line sample component separation with parallel biophysical and SAXS characterization of the separated components. The system coupled to an automated data analysis pipeline provides a novel tool to study difficult samples at the P12 synchrotron beamline (PETRA-3, EMBL/DESY, Hamburg).
Contact author
  • Melissa Graewert (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Models

Model #247
Type: dummy / Software: DAMMIN / Radius of dummy atoms: 3.75 A / Symmetry: P1
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: PlaB / Specimen concentration: 0.10-4.50
BufferName: Tris / Concentration: 100.00 mM / pH: 7.5 / Composition: 100 mM Nacl
Entity #146Type: protein / Description: PlaB / Formula weight: 55 / Num. of mol.: 4 / Source: Legionella pneumophila

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Experimental information

BeamInstrument name: PETRA III P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.12 Å / Dist. spec. to detc.: 3.1 mm
DetectorName: Pilatus 2M
Scan
Title: Lpn PlaB / Measurement date: Nov 15, 2013 / Exposure time: 1 sec. / Number of frames: 160 / Unit: 1/nm /
MinMax
Q0.0844 3.2452
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 781 /
MinMax
Q0.134424 2.18895
P(R) point1 781
R0 10.8
Result
D max: 10.5 / Type of curve: merged
ExperimentalStandardPorod
MW225 kDa225 kDa170 kDa
Volume--270 nm3

GuinierP(R)
Forward scattering, I01380.54 -
Radius of gyration, Rg3.95 nm3.9 nm

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