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- PDB-8xou: Prohead portal vertex of bacteriophage lambda -

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Basic information

Entry
Database: PDB / ID: 8xou
TitleProhead portal vertex of bacteriophage lambda
Components
  • Major capsid protein
  • Portal protein B
KeywordsVIRAL PROTEIN / caudovirales / siphoviridae / portal vertex / portal / capsid / connector/neck / tail / delivery device / B-DNA / phage lambda
Function / homology
Function and homology information


symbiont genome ejection through host cell envelope, long flexible tail mechanism / viral portal complex / T=7 icosahedral viral capsid / virion assembly / viral capsid / host cell cytoplasm / structural molecule activity / DNA binding
Similarity search - Function
Phage portal protein, lambda family / Phage portal protein, lambda family / Major capsid protein GpE / Phage major capsid protein E
Similarity search - Domain/homology
Portal protein B / Major capsid protein
Similarity search - Component
Biological speciesEscherichia phage Lambda (virus)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 5.58 Å
AuthorsWang, J.W. / Gu, Z.W.
Funding support China, 2items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32371254 China
National Natural Science Foundation of China (NSFC)32171190 China
CitationJournal: J Virol / Year: 2024
Title: Structural morphing in the viral portal vertex of bacteriophage lambda.
Authors: Zhiwei Gu / Kexun Wu / Jiawei Wang /
Abstract: The portal protein of tailed bacteriophage plays essential roles in various aspects of capsid assembly, motor assembly, genome packaging, connector formation, and infection processes. After DNA ...The portal protein of tailed bacteriophage plays essential roles in various aspects of capsid assembly, motor assembly, genome packaging, connector formation, and infection processes. After DNA packaging is complete, additional proteins are assembled onto the portal to form the connector complex, which is crucial as it bridges the mature head and tail. In this study, we report high-resolution cryo-electron microscopy (cryo-EM) structures of the portal vertex from bacteriophage lambda in both its prohead and mature virion states. Comparison of these structures shows that during head maturation, in addition to capsid expansion, the portal protein undergoes conformational changes to establish interactions with the connector proteins. Additionally, the independently assembled tail undergoes morphological alterations at its proximal end, facilitating its connection to the head-tail joining protein and resulting in the formation of a stable portal-connector-tail complex. The B-DNA molecule spirally glides through the tube, interacting with the nozzle blade region of the middle-ring connector protein. These insights elucidate a mechanism for portal maturation and DNA translocation within the phage lambda system.
IMPORTANCE: The tailed bacteriophages possess a distinct portal vertex that consists of a ring of 12 portal proteins associated with a 5-fold capsid shell. This portal protein is crucial in multiple ...IMPORTANCE: The tailed bacteriophages possess a distinct portal vertex that consists of a ring of 12 portal proteins associated with a 5-fold capsid shell. This portal protein is crucial in multiple stages of virus assembly and infection. Our research focused on examining the structures of the portal vertex in both its preliminary prohead state and the fully mature virion state of bacteriophage lambda. By analyzing these structures, we were able to understand how the portal protein undergoes conformational changes during maturation, the mechanism by which it prevents DNA from escaping, and the process of DNA spirally gliding.
History
DepositionJan 2, 2024Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Apr 10, 2024Provider: repository / Type: Initial release
Revision 1.1May 8, 2024Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
B: Portal protein B
b: Portal protein B
B1: Portal protein B
b1: Portal protein B
B2: Portal protein B
b2: Portal protein B
B3: Portal protein B
b3: Portal protein B
B4: Portal protein B
b4: Portal protein B
B5: Portal protein B
b5: Portal protein B
A0: Major capsid protein
G0: Major capsid protein
C0: Major capsid protein
D0: Major capsid protein
E0: Major capsid protein
F0: Major capsid protein
A1: Major capsid protein
G1: Major capsid protein
C1: Major capsid protein
D1: Major capsid protein
E1: Major capsid protein
F1: Major capsid protein
A2: Major capsid protein
G2: Major capsid protein
C2: Major capsid protein
D2: Major capsid protein
E2: Major capsid protein
F2: Major capsid protein
A3: Major capsid protein
G3: Major capsid protein
C3: Major capsid protein
D3: Major capsid protein
E3: Major capsid protein
F3: Major capsid protein
A4: Major capsid protein
G4: Major capsid protein
C4: Major capsid protein
D4: Major capsid protein
E4: Major capsid protein
F4: Major capsid protein


Theoretical massNumber of molelcules
Total (without water)1,861,23042
Polymers1,861,23042
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1chain "G2"
d_2ens_1chain "A1"
d_3ens_1chain "A2"
d_4ens_1chain "A3"
d_5ens_1chain "A0"
d_6ens_1chain "C0"
d_7ens_1chain "C1"
d_8ens_1chain "C2"
d_9ens_1chain "C3"
d_10ens_1chain "C4"
d_11ens_1chain "D0"
d_12ens_1chain "D1"
d_13ens_1chain "D2"
d_14ens_1chain "D3"
d_15ens_1chain "D4"
d_16ens_1chain "E0"
d_17ens_1chain "E1"
d_18ens_1chain "E2"
d_19ens_1chain "E3"
d_20ens_1chain "E4"
d_21ens_1chain "F0"
d_22ens_1chain "F1"
d_23ens_1chain "F2"
d_24ens_1chain "F3"
d_25ens_1chain "F4"
d_26ens_1chain "G0"
d_27ens_1chain "G1"
d_28ens_1chain "A4"
d_29ens_1chain "G3"
d_30ens_1chain "G4"
d_1ens_2chain "b4"
d_2ens_2chain "B1"
d_3ens_2chain "B2"
d_4ens_2chain "B"
d_5ens_2chain "B4"
d_6ens_2chain "B5"
d_7ens_2chain "b"
d_8ens_2chain "b1"
d_9ens_2chain "b2"
d_10ens_2chain "b3"
d_11ens_2chain "B3"
d_12ens_2chain "b5"

NCS domain segments:

Component-ID: 1

Dom-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
d_1ens_1ALAALAALAALAG2Z7 - 3417 - 341
d_2ens_1ALAALAALAALAA1S7 - 3417 - 341
d_3ens_1ALAALAALAALAA2Y7 - 3417 - 341
d_4ens_1ALAALAALAALAA3EA7 - 3417 - 341
d_5ens_1ALAALAALAALAA0M7 - 3417 - 341
d_6ens_1ALAALAALAALAC0O7 - 3417 - 341
d_7ens_1ALAALAALAALAC1U7 - 3417 - 341
d_8ens_1ALAALAALAALAC2AA7 - 3417 - 341
d_9ens_1ALAALAALAALAC3GA7 - 3417 - 341
d_10ens_1ALAALAALAALAC4MA7 - 3417 - 341
d_11ens_1ALAALAALAALAD0P7 - 3417 - 341
d_12ens_1ALAALAALAALAD1V7 - 3417 - 341
d_13ens_1ALAALAALAALAD2BA7 - 3417 - 341
d_14ens_1ALAALAALAALAD3HA7 - 3417 - 341
d_15ens_1ALAALAALAALAD4NA7 - 3417 - 341
d_16ens_1ALAALAALAALAE0Q7 - 3417 - 341
d_17ens_1ALAALAALAALAE1W7 - 3417 - 341
d_18ens_1ALAALAALAALAE2CA7 - 3417 - 341
d_19ens_1ALAALAALAALAE3IA7 - 3417 - 341
d_20ens_1ALAALAALAALAE4OA7 - 3417 - 341
d_21ens_1ALAALAALAALAF0R7 - 3417 - 341
d_22ens_1ALAALAALAALAF1X7 - 3417 - 341
d_23ens_1ALAALAALAALAF2DA7 - 3417 - 341
d_24ens_1ALAALAALAALAF3JA7 - 3417 - 341
d_25ens_1ALAALAALAALAF4PA7 - 3417 - 341
d_26ens_1ALAALAALAALAG0N7 - 3417 - 341
d_27ens_1ALAALAALAALAG1T7 - 3417 - 341
d_28ens_1ALAALAALAALAA4KA7 - 3417 - 341
d_29ens_1ALAALAALAALAG3FA7 - 3417 - 341
d_30ens_1ALAALAALAALAG4LA7 - 3417 - 341
d_1ens_2GLNGLNPROPROb4J34 - 50734 - 507
d_2ens_2GLNGLNPROPROB1C34 - 50734 - 507
d_3ens_2GLNGLNPROPROB2E34 - 50734 - 507
d_4ens_2GLNGLNPROPROBA34 - 50734 - 507
d_5ens_2GLNGLNPROPROB4I34 - 50734 - 507
d_6ens_2GLNGLNPROPROB5K34 - 50734 - 507
d_7ens_2GLNGLNPROPRObB34 - 50734 - 507
d_8ens_2GLNGLNPROPROb1D34 - 50734 - 507
d_9ens_2GLNGLNPROPROb2F34 - 50734 - 507
d_10ens_2GLNGLNPROPROb3H34 - 50734 - 507
d_11ens_2GLNGLNPROPROB3G34 - 50734 - 507
d_12ens_2GLNGLNPROPROb5L34 - 50734 - 507

NCS ensembles :
ID
ens_1
ens_2

NCS oper:
IDCodeMatrixVector
1given(-0.503485874053, 0.683494769309, -0.528523296512), (-0.863867154973, -0.387368861672, 0.321992086186), (0.0153464389301, 0.618692383447, 0.785483431701)191.610244463, 375.960302695, -98.4765435246
2given(0.665922413522, 0.576903836874, -0.473000319417), (-0.745799649402, 0.530254270341, -0.403253384036), (0.0181720147602, 0.621298939147, 0.783362882765)-22.5735705485, 255.321784202, -99.2996125
3given(0.914993463171, -0.328747226076, 0.233906442196), (0.402855200638, 0.71244787889, -0.574565668296), (0.0222407210496, 0.619954257355, 0.784322681754)25.9758680885, 14.3415185732, -99.9406744938
4given(-0.977149374497, -0.156278783961, 0.144069572098), (0.211818116308, -0.772299433018, 0.598904559478), (0.0176687725821, 0.615735761056, 0.787754585535)372.532063875, 209.539255308, -98.6090990648
5given(-0.105215233297, 0.993000483155, 0.0536637227283), (-0.852414776535, -0.0622635411946, -0.519145740792), (-0.512170678023, -0.10036579045, 0.85299935796)30.6405735643, 341.034635561, 104.418542742
6given(0.780420104118, 0.366512920836, 0.50656958056), (-0.36341498098, 0.925169664614, -0.109501795767), (-0.508796631954, -0.0986375716306, 0.85521729214)-39.9906634631, 91.4117404407, 103.499304202
7given(0.589022983197, -0.766185156856, 0.256928454398), (0.629983975904, 0.634486359981, 0.44782502063), (-0.506134483482, -0.101918420377, 0.856411419948)175.589158905, -52.901678217, 103.507727453
8given(-0.414902209885, -0.839744261199, -0.350265230956), (0.754938359689, -0.532599699187, 0.382629891012), (-0.507862411783, -0.105674671565, 0.854931947285)379.456588652, 107.530524784, 104.432266926
9given(-0.84396479813, 0.24749177092, -0.475889948249), (-0.161233730389, -0.963214671451, -0.214991118157), (-0.511592712714, -0.104715424024, 0.852823297213)289.873349373, 350.996096509, 104.994971014
10given(0.562970300365, 0.664255898275, 0.49176065572), (-0.161063247436, 0.671777309703, -0.723031034254), (-0.810631279456, 0.327840430356, 0.485177885927)-34.8710778049, 133.66633399, 104.842210354
11given(0.330705020897, -0.43506092057, 0.837470109644), (0.485749072046, 0.839295648229, 0.244193885814), (-0.809124235306, 0.326044184515, 0.488889723337)136.979512999, -34.9749733526, 104.683382853
12given(-0.356221699028, -0.93408254091, 0.0244112250619), (0.464732632985, -0.154444863352, 0.871877493701), (-0.81063535636, 0.321926375048, 0.489115250292)350.470732735, 76.3494658831, 105.47646622
13given(-0.548503664008, -0.143177895374, -0.82379841032), (-0.195068289812, -0.936132335731, 0.292582658932), (-0.813075699354, 0.321179607501, 0.485542548956)310.565874052, 313.793935161, 106.125241799
14given(0.019589627093, 0.844649718359, -0.534960839488), (-0.581831136878, -0.425498658666, -0.693125832466), (-0.813073658822, 0.324834950011, 0.483108145845)72.4112299379, 349.217407721, 105.733183984
15given(0.515387701521, -0.315974472403, 0.796577460082), (0.625296773235, 0.774280072818, -0.097438771639), (-0.585985889337, 0.548316059975, 0.596632245081)138.52976686, -75.7229628241, 1.38505389944
16given(-0.432851825475, -0.836424982145, 0.336203132683), (0.683464337496, -0.0613122706911, 0.727404498772), (-0.587805917419, 0.544641216496, 0.59820577458)390.158300175, 65.2460762032, 2.35072340983
17given(-0.781189553086, -0.202554524977, -0.590520572512), (-0.200378215588, -0.814501551715, 0.544459174753), (-0.591262592142, 0.543653277987, 0.595692588898)333.84254236, 348.118935122, 3.10377901479
18given(-0.0482398239443, 0.709649057248, -0.702901938348), (-0.804798514808, -0.444411047055, -0.393443988159), (-0.591584541675, 0.546714767317, 0.592562817977)47.4116257882, 381.975830294, 2.60554451273
19given(0.753089304367, 0.639551161129, 0.154372315997), (-0.294502870031, 0.537510636064, -0.790158449719), (-0.588323515756, 0.549596787124, 0.593143163487)-73.2985281558, 120.026505286, 1.54309664288
20given(-0.312977004358, -0.651201454005, 0.691362467194), (0.917757237558, -0.0199875059712, 0.3966385666), (-0.244472999843, 0.758641658431, 0.603900477265)321.222339758, -3.44513423904, -78.7773811165
21given(-0.968471760996, -0.185555532747, -0.166227532077), (-0.0140176618224, -0.62560383568, 0.780014965203), (-0.248728674082, 0.757752588283, 0.603278593719)378.224986095, 261.342559888, -77.8403209617
22given(-0.284854315872, 0.534312342912, -0.795844418804), (-0.925368684758, -0.369898469063, 0.0828729138802), (-0.250101611348, 0.760056210288, 0.599803085357)144.010131438, 397.380434989, -77.913658807
23given(0.793140284607, 0.513566599588, -0.327380263181), (-0.556839359745, 0.393753812488, -0.731360282344), (-0.246694986516, 0.762369518631, 0.598276107403)-57.7443616384, 216.66846899, -78.8956905218
24given(0.775758913702, -0.219120538489, 0.591763717564), (0.582274537752, 0.610009724685, -0.537442553652), (-0.243216920666, 0.761494796701, 0.60080879163)51.778587336, -31.0554834784, -79.4296785283
25given(-0.809009037775, 0.587792824192, -0.0019931451711), (-0.587769326928, -0.808999821794, -0.00681957913892), (-0.00562095377009, -0.00434559156151, 0.999974760038)203.99540379, 402.149055764, 1.67054194233
26given(0.309019256645, 0.951054627651, 0.00148129847242), (-0.95104565867, 0.309022778868, -0.00413246493903), (-0.00438795487415, -0.000131771237817, 0.999990364198)-44.0923533646, 275.180910385, 0.756530033913
27given(-0.100481061296, -0.781880556359, 0.615277459289), (0.994697256976, -0.0925752755016, 0.0448016219662), (0.0219299631949, 0.616516515557, 0.787036506625)270.164575116, -13.9540174256, -99.5142406099
28given(0.309019836325, -0.951045465213, -0.00438906090004), (0.951054441536, 0.309023352452, -0.000129899017634), (0.00147986218511, -0.00413409448999, 0.999990359589)275.338416714, -43.103096881, 0.44689400119
29given(-0.809008923295, -0.587769474002, -0.00562205123884), (0.587792979398, -0.808999707276, -0.00434591731528), (-0.00199384027205, -0.00682048813597, 0.999974752453)401.414469196, 205.438492902, 1.47921460537
30given(-0.866233749962, 0.499586408771, -0.00724641975886), (-0.499527255179, -0.866255662338, -0.00858188795127), (-0.0105646467298, -0.00381413680976, 0.99993691831)229.10615342, 397.273306993, 2.42127965231
31given(-0.865613489083, -0.500596945917, -0.0107696451225), (0.500654335359, -0.865640845565, -0.00334110392227), (-0.00765009829079, -0.0082839741444, 0.999936423863)397.07001864, 229.353957507, 2.65979893816
32given(0.000659860091524, 0.999997810528, -0.00198583070215), (-0.999963745985, 0.000642976330675, -0.00849077720052), (-0.00848948176807, 0.00199136143283, 0.999961980867)-0.243637452439, 335.641692543, 1.08253343028
33given(0.866038702382, -0.499972161246, -0.00219179284802), (0.499975890999, 0.866037607939, 0.00172338459993), (0.00103653071211, -0.0025883613447, 0.999996112987)106.436102572, -61.3258242374, 0.264292967823
34given(0.865744625055, 0.500484733794, 0.00112934847084), (-0.500478714792, 0.865741986983, -0.00344499850986), (-0.00270189355127, 0.002417274072, 0.999993428257)-61.5644384557, 106.564145949, 0.0380619591809
35given(-0.500661551902, 0.865629095071, -0.00492749558685), (-0.865583698153, -0.500685769155, -0.00886690798484), (-0.0101425804529, -0.000174160059568, 0.999948547541)106.379641129, 397.238547252, 1.74542054495
36given(-0.999954256576, -0.000263845247988, -0.00956112656656), (0.000328425138128, -0.999977141046, -0.00675348219967), (-0.00955912613502, -0.00675631338659, 0.999931485321)335.518170838, 335.916095441, 2.74190550275
37given(-0.499272484906, -0.866389285472, -0.00982811452798), (0.866434795038, -0.499289595621, -0.000803524735947), (-0.00421091010656, -0.00891659818826, 0.999951380074)397.012398115, 106.518970924, 2.21463650282
38given(0.500697045378, -0.865604738897, -0.00555920398345), (0.86562239135, 0.500690958301, 0.00253768959118), (0.000586807033895, -0.00608278512655, 0.999981327517)229.223559939, -61.3510645415, 0.941177701407
39given(-0.000128814840256, -0.999964957448, -0.00837062018429), (0.999998753227, -0.000141984130489, 0.00157270011784), (-0.00157383350164, -0.00837040716091, 0.999963729008)335.745656066, 0.239957480368, 1.66777063319
40given(0.499901550875, 0.86608214182, 0.000403798902482), (-0.866068597503, 0.499896240957, -0.00537891218397), (-0.00486043733841, 0.00233920899364, 0.999985452019)-61.5789607979, 229.207299598, 0.444447828918

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Components

#1: Protein
Portal protein B / GpB / Minor capsid protein B


Mass: 59529.609 Da / Num. of mol.: 12
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia phage Lambda (virus) / Gene: B / Production host: Escherichia phage Lambda (virus) / References: UniProt: P03710
#2: Protein ...
Major capsid protein / Gene product E / gpE / Major head protein


Mass: 38229.160 Da / Num. of mol.: 30
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia phage Lambda (virus) / Gene: E / Production host: Escherichia phage Lambda (virus) / References: UniProt: P03713

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Escherichia phage Lambda / Type: VIRUS / Entity ID: all / Source: NATURAL
Source (natural)Organism: Escherichia phage Lambda (virus)
Details of virusEmpty: YES / Enveloped: NO / Isolate: STRAIN / Type: VIRION
Buffer solutionpH: 7
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: NITROGEN

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2000 nm / Nominal defocus min: 1000 nm
Image recordingElectron dose: 50 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k)

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Processing

CTF correctionType: NONE
3D reconstructionResolution: 5.58 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 50921 / Symmetry type: POINT
RefinementCross valid method: NONE
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
Displacement parametersBiso mean: 354.63 Å2
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.0042126138
ELECTRON MICROSCOPYf_angle_d0.95170706
ELECTRON MICROSCOPYf_chiral_restr0.051618330
ELECTRON MICROSCOPYf_plane_restr0.009222602
ELECTRON MICROSCOPYf_dihedral_angle_d6.944417418
Refine LS restraints NCS
Ens-IDDom-IDAsym-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2ZG2ELECTRON MICROSCOPYNCS constraints3.7268497101E-10
ens_1d_3ZG2ELECTRON MICROSCOPYNCS constraints6.7536465702E-11
ens_1d_4ZG2ELECTRON MICROSCOPYNCS constraints3.37770689455E-13
ens_1d_5ZG2ELECTRON MICROSCOPYNCS constraints2.56285863562E-11
ens_1d_6ZG2ELECTRON MICROSCOPYNCS constraints1.07542587476E-12
ens_1d_7ZG2ELECTRON MICROSCOPYNCS constraints8.86521759109E-11
ens_1d_8ZG2ELECTRON MICROSCOPYNCS constraints1.53718483024E-12
ens_1d_9ZG2ELECTRON MICROSCOPYNCS constraints5.7793781758E-13
ens_1d_10ZG2ELECTRON MICROSCOPYNCS constraints3.23028370234E-13
ens_1d_11ZG2ELECTRON MICROSCOPYNCS constraints3.20943597374E-13
ens_1d_12ZG2ELECTRON MICROSCOPYNCS constraints3.38971022399E-13
ens_1d_13ZG2ELECTRON MICROSCOPYNCS constraints2.95333980263E-10
ens_1d_14ZG2ELECTRON MICROSCOPYNCS constraints6.76239361598E-13
ens_1d_15ZG2ELECTRON MICROSCOPYNCS constraints5.04018332296E-13
ens_1d_16ZG2ELECTRON MICROSCOPYNCS constraints2.30100268447E-11
ens_1d_17ZG2ELECTRON MICROSCOPYNCS constraints1.61625108101E-10
ens_1d_18ZG2ELECTRON MICROSCOPYNCS constraints7.48756422628E-11
ens_1d_19ZG2ELECTRON MICROSCOPYNCS constraints7.93199340951E-13
ens_1d_20ZG2ELECTRON MICROSCOPYNCS constraints4.62303008248E-13
ens_1d_21ZG2ELECTRON MICROSCOPYNCS constraints8.48841434027E-11
ens_1d_22ZG2ELECTRON MICROSCOPYNCS constraints5.92816914564E-13
ens_1d_23ZG2ELECTRON MICROSCOPYNCS constraints6.83252122772E-13
ens_1d_24ZG2ELECTRON MICROSCOPYNCS constraints8.20085928914E-13
ens_1d_25ZG2ELECTRON MICROSCOPYNCS constraints4.16136389111E-11
ens_1d_26ZG2ELECTRON MICROSCOPYNCS constraints2.6577608974E-11
ens_1d_27ZG2ELECTRON MICROSCOPYNCS constraints3.32471027443E-10
ens_1d_28ZG2ELECTRON MICROSCOPYNCS constraints3.44796186641E-12
ens_1d_29ZG2ELECTRON MICROSCOPYNCS constraints5.78853117977E-13
ens_1d_30ZG2ELECTRON MICROSCOPYNCS constraints8.15714255985E-12
ens_2d_2Jb4ELECTRON MICROSCOPYNCS constraints3.45777884273E-11
ens_2d_3Jb4ELECTRON MICROSCOPYNCS constraints3.72947434633E-11
ens_2d_4Jb4ELECTRON MICROSCOPYNCS constraints5.68448489728E-12
ens_2d_5Jb4ELECTRON MICROSCOPYNCS constraints1.55575274643E-11
ens_2d_6Jb4ELECTRON MICROSCOPYNCS constraints2.86423235599E-13
ens_2d_7Jb4ELECTRON MICROSCOPYNCS constraints7.26109788393E-11
ens_2d_8Jb4ELECTRON MICROSCOPYNCS constraints5.76752425835E-12
ens_2d_9Jb4ELECTRON MICROSCOPYNCS constraints7.25448216678E-13
ens_2d_10Jb4ELECTRON MICROSCOPYNCS constraints1.23120159927E-10
ens_2d_11Jb4ELECTRON MICROSCOPYNCS constraints9.69328281573E-13
ens_2d_12Jb4ELECTRON MICROSCOPYNCS constraints4.91435204128E-11

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