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Open data
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Basic information
Entry | Database: PDB / ID: 8xj8 | ||||||
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Title | The Cryo-EM structure of MPXV E5 C-terminal in complex with DNA | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhang, W. / Liu, Y. / Gao, H. / Gan, J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural and functional insights into the helicase protein E5 of Mpox virus. Authors: Gan, J. / Zhang, W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 404.8 KB | Display | ![]() |
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PDB format | ![]() | 323.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 38396MC ![]() 8xifC ![]() 8xigC ![]() 8xj6C ![]() 8xj7C M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
#1: DNA chain | Mass: 21440.668 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() ![]() | ||||||
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#2: Protein | Mass: 53282.012 Da / Num. of mol.: 6 / Fragment: C-terminal Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() References: UniProt: A0A7H0DN89, ![]() #3: Chemical | #4: Chemical | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: ![]() |
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Sample preparation
Component | Name: Monkeypox virus![]() |
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Source (natural) | Organism: ![]() ![]() |
Source (recombinant) | Organism: ![]() ![]() ![]() |
Details of virus | Empty: YES / Enveloped: YES / Isolate: SPECIES / Type: VIRION |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied![]() ![]() |
Vitrification![]() | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source![]() ![]() |
Electron lens | Mode: BRIGHT FIELD![]() |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
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Processing
EM software | Name: PHENIX / Version: 1.19.1_4122: / Category: model refinement | ||||||||||||||||||||||||
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CTF correction![]() | Type: NONE | ||||||||||||||||||||||||
3D reconstruction![]() | Resolution: 2.67 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 247898 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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