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Open data
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Basic information
Entry | Database: PDB / ID: 7ylw | ||||||
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Title | yeast TRiC-plp2-tubulin complex at S3 closed TRiC state | ||||||
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Function / homology | ![]() negative regulation of chaperone-mediated protein folding / Association of TriC/CCT with target proteins during biosynthesis / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / response to pheromone / chaperone mediated protein folding independent of cofactor / chaperonin-containing T-complex / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Han, W.Y. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis of plp2-mediated cytoskeletal protein folding by TRiC/CCT. Authors: Wenyu Han / Mingliang Jin / Caixuan Liu / Qiaoyu Zhao / Shutian Wang / Yifan Wang / Yue Yin / Chao Peng / Yanxing Wang / Yao Cong / ![]() Abstract: The cytoskeletal proteins tubulin and actin are the obligate substrates of TCP-1 ring complex/Chaperonin containing TCP-1 (TRiC/CCT), and their folding involves co-chaperone. Through cryo-electron ...The cytoskeletal proteins tubulin and actin are the obligate substrates of TCP-1 ring complex/Chaperonin containing TCP-1 (TRiC/CCT), and their folding involves co-chaperone. Through cryo-electron microscopy analysis, we present a more complete picture of TRiC-assisted tubulin/actin folding along TRiC adenosine triphosphatase cycle, under the coordination of co-chaperone plp2. In the open S1/S2 states, plp2 and tubulin/actin engaged within opposite TRiC chambers. Notably, we captured an unprecedented TRiC-plp2-tubulin complex in the closed S3 state, engaged with a folded full-length -tubulin and loaded with a guanosine triphosphate, and a plp2 occupying opposite rings. Another closed S4 state revealed an actin in the intermediate folding state and a plp2. Accompanying TRiC ring closure, plp2 translocation could coordinate substrate translocation on the CCT6 hemisphere, facilitating substrate stabilization and folding. Our findings reveal the folding mechanism of the major cytoskeletal proteins tubulin/actin under the coordination of the biogenesis machinery TRiC and plp2 and extend our understanding of the links between cytoskeletal proteostasis and related human diseases. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1.5 MB | Display | ![]() |
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PDB format | ![]() | 1.2 MB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 33919MC ![]() 7yluC ![]() 7ylvC ![]() 7ylxC ![]() 7ylyC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Components
-T-complex protein 1 subunit ... , 8 types, 16 molecules AaBbDdEeGgHhQqZz
#1: Protein | Mass: 60557.566 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: ATCC 204508 / S288c / Gene: TCP1, CCT1, YDR212W, YD8142.13, YD8142B.04 / Production host: ![]() ![]() ![]() #2: Protein | Mass: 57276.254 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: ATCC 204508 / S288c / Gene: CCT2, BIN3, TCP2, YIL142W / Production host: ![]() ![]() ![]() #3: Protein | Mass: 57682.410 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Gene: CCT4 / Production host: ![]() ![]() ![]() #4: Protein | Mass: 61995.004 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: ATCC 204508 / S288c / Gene: CCT5, TCP5, YJR064W, J1752 / Production host: ![]() ![]() ![]() #5: Protein | Mass: 65423.387 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: fusion protein of T-complex protein 1 subunit gamma,rep-His-CBP and T-complex protein 1 subunit gamma Source: (gene. exp.) ![]() ![]() ![]() Strain: ATCC 204508 / S288c / Gene: CCT3, BIN2, TCP3, YJL014W, J1336 / Production host: ![]() ![]() ![]() #6: Protein | Mass: 59802.438 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Gene: CCT7 / Production host: ![]() ![]() ![]() #7: Protein | Mass: 61735.102 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: ATCC 204508 / S288c / Gene: CCT8, YJL008C, J1374 / Production host: ![]() ![]() ![]() #9: Protein | Mass: 59997.559 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: ATCC 204508 / S288c / Gene: CCT6, TCP20, TCP6, YDR188W, YD9395.21 / Production host: ![]() ![]() ![]() |
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-Protein , 2 types, 2 molecules Tp
#8: Protein | Mass: 50967.457 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Gene: TUB2, GI527_G0002040 / Production host: ![]() ![]() ![]() |
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#10: Protein | Mass: 32836.270 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: ATCC 204508 / S288c / Gene: PLP2, VIAF1, YOR281C / Production host: ![]() ![]() ![]() |
-Non-polymers , 5 types, 61 molecules ![](data/chem/img/ADP.gif)
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![](data/chem/img/GTP.gif)
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![](data/chem/img/MG.gif)
![](data/chem/img/AF3.gif)
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#11: Chemical | ChemComp-ADP / ![]() #12: Chemical | ChemComp-MG / #13: Chemical | ChemComp-AF3 / ![]() #14: Chemical | ChemComp-GTP / | ![]() #15: Water | ChemComp-HOH / | ![]() |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: ![]() |
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Sample preparation
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Source (recombinant) | Organism: ![]() ![]() ![]() | ||||||||||||||||||||||||
Buffer solution | pH: 7.5 | ||||||||||||||||||||||||
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied![]() ![]() | ||||||||||||||||||||||||
Vitrification![]() | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source![]() ![]() |
Electron lens | Mode: BRIGHT FIELD![]() |
Image recording | Electron dose: 38 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
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Processing
CTF correction![]() | Type: NONE |
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3D reconstruction![]() | Resolution: 3.39 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 28063 / Symmetry type: POINT |