ジャーナル: Cell Rep / 年: 2020 タイトル: Structure of Human ATG9A, the Only Transmembrane Protein of the Core Autophagy Machinery. 著者: Carlos M Guardia / Xiao-Feng Tan / Tengfei Lian / Mitra S Rana / Wenchang Zhou / Eric T Christenson / Augustus J Lowry / José D Faraldo-Gómez / Juan S Bonifacino / Jiansen Jiang / Anirban Banerjee / 要旨: Autophagy is a catabolic process involving capture of cytoplasmic materials into double-membraned autophagosomes that subsequently fuse with lysosomes for degradation of the materials by lysosomal ...Autophagy is a catabolic process involving capture of cytoplasmic materials into double-membraned autophagosomes that subsequently fuse with lysosomes for degradation of the materials by lysosomal hydrolases. One of the least understood components of the autophagy machinery is the transmembrane protein ATG9. Here, we report a cryoelectron microscopy structure of the human ATG9A isoform at 2.9-Å resolution. The structure reveals a fold with a homotrimeric domain-swapped architecture, multiple membrane spans, and a network of branched cavities, consistent with ATG9A being a membrane transporter. Mutational analyses support a role for the cavities in the function of ATG9A. In addition, structure-guided molecular simulations predict that ATG9A causes membrane bending, explaining the localization of this protein to small vesicles and highly curved edges of growing autophagosomes.
#203 - 2016年11月 アミノペプチダーゼ1とオートファジー (Aminopeptidase 1 and Autophagy) 類似性 (1)
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C: Autophagy-related protein 9A F: Autophagy-related protein 9A B: Autophagy-related protein 9A A: Autophagy-related protein 9A E: Autophagy-related protein 9A D: Autophagy-related protein 9A ヘテロ分子