mRNA modification / Noc4p-Nop14p complex / rRNA acetylation involved in maturation of SSU-rRNA / rRNA cytidine N-acetyltransferase activity / tRNA acetylation / box H/ACA snoRNA binding / regulation of ribosomal protein gene transcription by RNA polymerase II / rRNA small subunit pseudouridine methyltransferase Nep1 / RNA fragment catabolic process / rRNA 2'-O-methylation ...mRNA modification / Noc4p-Nop14p complex / rRNA acetylation involved in maturation of SSU-rRNA / rRNA cytidine N-acetyltransferase activity / tRNA acetylation / box H/ACA snoRNA binding / regulation of ribosomal protein gene transcription by RNA polymerase II / rRNA small subunit pseudouridine methyltransferase Nep1 / RNA fragment catabolic process / rRNA 2'-O-methylation / Pwp2p-containing subcomplex of 90S preribosome / CURI complex / histone H2AQ104 methyltransferase activity / nuclear microtubule / endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / t-UTP complex / UTP-C complex / Mpp10 complex / snoRNA guided rRNA 2'-O-methylation / rRNA (pseudouridine) methyltransferase activity / rRNA modification / regulation of rRNA processing / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / box C/D sno(s)RNA binding / snRNA binding / box C/D sno(s)RNA 3'-end processing / positive regulation of RNA binding / septum digestion after cytokinesis / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rDNA heterochromatin / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / tRNA export from nucleus / rRNA methyltransferase activity / regulation of transcription by RNA polymerase I / positive regulation of rRNA processing / single-stranded telomeric DNA binding / U4/U6 snRNP / rRNA base methylation / 90S preribosome assembly / rRNA primary transcript binding / SUMOylation of RNA binding proteins / small nuclear ribonucleoprotein complex / O-methyltransferase activity / sno(s)RNA-containing ribonucleoprotein complex / protein localization to nucleolus / U4 snRNA binding / box C/D methylation guide snoRNP complex / mTORC1-mediated signalling / Protein hydroxylation / rRNA methylation / poly(U) RNA binding / U4 snRNP / U3 snoRNA binding / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / preribosome, small subunit precursor / poly(A)+ mRNA export from nucleus / establishment of cell polarity / snoRNA binding / precatalytic spliceosome / positive regulation of transcription by RNA polymerase I / spliceosomal complex assembly / Major pathway of rRNA processing in the nucleolus and cytosol / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / nucleolar large rRNA transcription by RNA polymerase I / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / L13a-mediated translational silencing of Ceruloplasmin expression / regulation of translational fidelity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal subunit export from nucleus / RNA processing / enzyme activator activity / 90S preribosome / vesicle-mediated transport / U4/U6 x U5 tri-snRNP complex / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / RNA endonuclease activity / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / nuclear periphery / maturation of LSU-rRNA / Transferases; Transferring one-carbon groups; Methyltransferases / maturation of SSU-rRNA / small-subunit processome / maintenance of translational fidelity / spliceosomal complex / mRNA splicing, via spliceosome / rRNA processing / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit rRNA binding / protein transport / ribosome biogenesis / small ribosomal subunit / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit Similarity search - Function
Regulator of rDNA transcription 14 / Regular of rDNA transcription protein 14 / Apoptosis-antagonizing transcription factor, C-terminal / AATF leucine zipper-containing domain / Protein AATF/Bfr2 / Apoptosis-antagonizing transcription factor, C-terminal / Apoptosis antagonizing transcription factor / NUC153 / Nucleolar protein 10/Enp2 / NUC153 domain ...Regulator of rDNA transcription 14 / Regular of rDNA transcription protein 14 / Apoptosis-antagonizing transcription factor, C-terminal / AATF leucine zipper-containing domain / Protein AATF/Bfr2 / Apoptosis-antagonizing transcription factor, C-terminal / Apoptosis antagonizing transcription factor / NUC153 / Nucleolar protein 10/Enp2 / NUC153 domain / U3 small nucleolar RNA-associated protein 8 / Utp8, N-terminal beta propeller / rRNA biogenesis protein Rrp5 / : / : / Possible tRNA binding domain / RNA cytidine acetyltransferase NAT10 / Possible tRNA binding domain / Helicase domain / tRNA(Met) cytidine acetyltransferase TmcA, N-terminal / TmcA/NAT10/Kre33 / : / : / : / Helicase / tRNA(Met) cytidine acetyltransferase TmcA, N-terminal / GNAT acetyltransferase 2 / KRR1 small subunit processome component, second KH domain / Ribosomal RNA assembly KRR1 / U3 small nucleolar RNA-associated protein 20, N-terminal / U3 snoRNA associated / Nucleolar complex protein 4 / U3 small nucleolar RNA-associated protein 20, C-terminal / U3 small nucleolar RNA-associated protein 20, N-terminal / U3 snoRNA associated / U3 small nucleolar RNA-associated protein 20, C-terminal / Nucleolar protein 14 / Nop14-like family / U3 small nucleolar RNA-associated protein 6 / rRNA-processing protein Fcf1, PIN domain / U3 small nucleolar RNA-associated protein 6 / NOL6/Upt22 / Small-subunit processome, Utp11 / Sof1-like protein / BING4, C-terminal domain / Fcf2 pre-rRNA processing, C-terminal / Nrap protein domain 1 / Nrap protein, domain 2 / Nrap protein, domain 3 / Nrap protein, domain 4 / Nrap protein, domain 5 / Nrap protein, domain 6 / Fcf2/DNTTIP2 / WD repeat-containing protein WDR46/Utp7 / Nrap protein domain 1 / Utp11 protein / Sof1-like domain / BING4CT (NUC141) domain / Fcf2 pre-rRNA processing / Nrap protein PAP/OAS-like domain / Nrap protein domain 3 / Nrap protein nucleotidyltransferase domain 4 / Nrap protein PAP/OAS1-like domain 5 / Nrap protein domain 6 / BING4CT (NUC141) domain / rRNA-processing protein Fcf1/Utp23 / Ribosomal RNA-processing protein 7, C-terminal domain / Ribosomal RNA-processing protein 7 / Rrp7, RRM-like N-terminal domain / Fcf1 / Ribosomal RNA-processing protein 7 (RRP7) C-terminal domain / Rrp7 RRM-like N-terminal domain / Small-subunit processome, Utp14 / U3 small nucleolar RNA-associated protein 15, C-terminal / Utp14 protein / UTP15 C terminal / Sas10 C-terminal domain / Sas10 C-terminal domain / Nucleolar protein 58/56, N-terminal / U3 small nucleolar RNA-associated protein 18 / NOP5NT (NUC127) domain / BP28, C-terminal domain / RNA 3'-terminal phosphate cyclase-like, conserved site / U3 small nucleolar RNA-associated protein 10, N-terminal / Ribosome biogenesis protein Bms1, N-terminal / U3 small nucleolar RNA-associated protein 10 / BP28CT (NUC211) domain / U3 small nucleolar RNA-associated protein 10 / RNA 3'-terminal phosphate cyclase signature. / BP28CT (NUC211) domain / U3 small nucleolar RNA-associated protein 13, C-terminal / Periodic tryptophan protein 2 / Utp13 specific WD40 associated domain / Small-subunit processome, Utp12 / Ribosomal RNA-processing protein Rrp9-like / Dip2/Utp12 Family / Sas10/Utp3/C1D / Small-subunit processome, Utp21 / U3 small nucleolar ribonucleoprotein complex, subunit Mpp10 / RNA 3'-terminal phosphate cyclase type 2 Similarity search - Domain/homology
GUANOSINE-5'-TRIPHOSPHATE / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein uS4A / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS11A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A ...GUANOSINE-5'-TRIPHOSPHATE / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein uS4A / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS11A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A / Small ribosomal subunit protein eS4A / Small ribosomal subunit protein eS6A / Small ribosomal subunit protein eS8A / Small ribosomal subunit protein uS17A / Small ribosomal subunit protein uS9A / Small ribosomal subunit protein uS13A / rRNA 2'-O-methyltransferase fibrillarin / Ribosomal RNA-processing protein 7 / KRR1 small subunit processome component / Periodic tryptophan protein 2 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS7A / U3 small nucleolar ribonucleoprotein protein IMP3 / Protein SOF1 / U3 small nucleolar RNA-associated protein 11 / U3 small nucleolar RNA-associated protein 20 / Ribosome biogenesis protein UTP30 / Essential nuclear protein 1 / U3 small nucleolar RNA-associated protein 9 / 13 kDa ribonucleoprotein-associated protein / U3 small nucleolar RNA-associated protein 7 / U3 small nucleolar ribonucleoprotein protein LCP5 / U3 small nucleolar RNA-associated protein 18 / Regulator of rDNA transcription protein 14 / Protein FAF1 / U3 small nucleolar RNA-associated protein 10 / U3 small nucleolar RNA-associated protein MPP10 / Ribosome biogenesis protein ENP2 / Small ribosomal subunit protein eS12 / U3 small nucleolar RNA-associated protein 22 / U3 small nucleolar RNA-associated protein 8 / RNA cytidine acetyltransferase / U3 small nucleolar ribonucleoprotein protein IMP4 / U3 small nucleolar RNA-associated protein 6 / NET1-associated nuclear protein 1 / U3 small nucleolar RNA-associated protein 5 / U3 small nucleolar RNA-associated protein 15 / U3 small nucleolar RNA-associated protein 14 / rRNA biogenesis protein RRP5 / rRNA-processing protein FCF1 / U3 small nucleolar RNA-associated protein 13 / U3 small nucleolar RNA-associated protein 21 / Ribosomal RNA small subunit methyltransferase NEP1 / Ribosomal RNA-processing protein 9 / Nucleolar complex protein 4 / Protein BFR2 / U3 small nucleolar RNA-associated protein 4 / RNA 3'-terminal phosphate cyclase-like protein / U3 small nucleolar RNA-associated protein 16 / Ribosome biogenesis protein BMS1 / rRNA-processing protein FCF2 / Something about silencing protein 10 / U3 small nucleolar RNA-associated protein 12 / Nucleolar protein 56 / Nucleolar protein 58 / Small ribosomal subunit protein eS28A / Small ribosomal subunit protein uS8B / Nucleolar complex protein 14 Similarity search - Component
National Natural Science Foundation of China (NSFC)
91940302, 91540201, 31430024, 31325007
China
Chinese Academy of Sciences
XDB08010203
China
Citation
Journal: Science / Year: 2020 Title: Cryo-EM structure of 90 small ribosomal subunit precursors in transition states. Authors: Yifei Du / Weidong An / Xing Zhu / Qi Sun / Jia Qi / Keqiong Ye / Abstract: The 90 preribosome is a large, early assembly intermediate of small ribosomal subunits that undergoes structural changes to give a pre-40 ribosome. Here, we gained insight into this transition by ...The 90 preribosome is a large, early assembly intermediate of small ribosomal subunits that undergoes structural changes to give a pre-40 ribosome. Here, we gained insight into this transition by determining cryo-electron microscopy structures of intermediates in the path from the 90 to the pre-40 The full transition is blocked by deletion of RNA helicase Dhr1. A series of structural snapshots revealed that the excised 5' external transcribed spacer (5' ETS) is degraded within 90, driving stepwise disassembly of assembly factors and ribosome maturation. The nuclear exosome, an RNA degradation machine, docks on the 90 through helicase Mtr4 and is primed to digest the 3' end of the 5' ETS. The structures resolved between 3.2- and 8.6-angstrom resolution reveal key intermediates and the critical role of 5' ETS degradation in 90 progression.
3A: U3 snoRNA 5A: 5' ETS SA: 18S pre-rRNA SF: 40S ribosomal protein S4-A SG: 40S ribosomal protein S5 SH: 40S ribosomal protein S6-A SI: 40S ribosomal protein S7-A SJ: 40S ribosomal protein S8-A SK: 40S ribosomal protein S9-A SM: 40S ribosomal protein S11-A SN: 40S ribosomal protein S12 SO: 40S ribosomal protein S13 SP: 40S ribosomal protein S14-A SR: 40S ribosomal protein S16-A ST: 40S ribosomal protein S18-A SX: 40S ribosomal protein S22-B SY: 40S ribosomal protein S23-A SZ: 40S ribosomal protein S24-A Sd: 40S ribosomal protein S28-A 3B: rRNA 2'-O-methyltransferase fibrillarin 3C: rRNA 2'-O-methyltransferase fibrillarin 3D: Nucleolar protein 56 3E: Nucleolar protein 58 3F: Ribosomal RNA-processing protein 9 3G: 13 kDa ribonucleoprotein-associated protein 3H: 13 kDa ribonucleoprotein-associated protein A4: U3 small nucleolar RNA-associated protein 4 A5: U3 small nucleolar RNA-associated protein 5 A8: U3 small nucleolar RNA-associated protein 8 A9: U3 small nucleolar RNA-associated protein 9 AE: U3 small nucleolar RNA-associated protein 10 AF: U3 small nucleolar RNA-associated protein 15 AG: NET1-associated nuclear protein 1 B1: Periodic tryptophan protein 2 B2: U3 small nucleolar RNA-associated protein 12 B3: U3 small nucleolar RNA-associated protein 13 B8: U3 small nucleolar RNA-associated protein 18 BE: U3 small nucleolar RNA-associated protein 21 B6: U3 small nucleolar RNA-associated protein 6 5B: Bud site selection protein 21 5C: U3 small nucleolar RNA-associated protein 7 5D: U3 small nucleolar RNA-associated protein 11 5E: U3 small nucleolar RNA-associated protein MPP10 5F: U3 small nucleolar ribonucleoprotein protein IMP3 5G: U3 small nucleolar ribonucleoprotein protein IMP4 5H: Something about silencing protein 10 5I: Protein SOF1 5J: rRNA-processing protein FCF2 5K: rRNA-processing protein FCF1 RA: Ribosome biogenesis protein ENP2 RB: U3 small nucleolar ribonucleoprotein protein LCP5 RC: KRR1 small subunit processome component RD: rRNA biogenesis protein RRP5 RE: U3 small nucleolar RNA-associated protein 22 RF: Ribosomal RNA-processing protein 7 RG: Ribosomal RNA small subunit methyltransferase NEP1 RH: Ribosomal RNA small subunit methyltransferase NEP1 RI: Ribosome biogenesis protein UTP30 RJ: Ribosome biogenesis protein BMS1 RK: RNA 3'-terminal phosphate cyclase-like protein RL: RNA cytidine acetyltransferase RM: RNA cytidine acetyltransferase RN: Nucleolar complex protein 14 RO: Nucleolar complex protein 4 RP: U3 small nucleolar RNA-associated protein 20 RQ: U3 small nucleolar RNA-associated protein 14 RS: Essential nuclear protein 1 RT: Pno1 RV: Protein FAF1 RW: Regulator of rDNA transcription protein 14 RY: Protein BFR2 X1: Unassigned helices hetero molecules
Mass: 7605.847 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q3E7X9
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Protein , 18 types, 22 molecules 3B3C3G3HAGB15B5H5IRCRDRGRHRKRLRMRSRTRVRWRYX1
#20: Protein
rRNA2'-O-methyltransferasefibrillarin / Nop1 / Histone-glutamine methyltransferase / U3 small nucleolar RNA-associated protein NOP1 / U3 ...Nop1 / Histone-glutamine methyltransferase / U3 small nucleolar RNA-associated protein NOP1 / U3 snoRNA-associated protein NOP1
Mass: 34525.418 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c References: UniProt: P15646, Transferases; Transferring one-carbon groups; Methyltransferases
#24: Protein
13kDaribonucleoprotein-associatedprotein / Snu13 / Small nuclear ribonucleoprotein-associated protein 1
Mass: 13582.855 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P39990
#31: Protein
NET1-associatednuclearprotein1 / Utp17 / U three protein 17 / t-17 / U3 protein 17 required for transcription / U3 small nucleolar ...Utp17 / U three protein 17 / t-17 / U3 protein 17 required for transcription / U3 small nucleolar RNA-associated protein 17 / U3 snoRNA-associated protein 17
Mass: 101341.734 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q02931
#32: Protein
Periodictryptophanprotein2 / Utp1 / U three protein 1 / U3 small nucleolar RNA-associated protein 1 / U3 snoRNA-associated protein 1
Mass: 104097.039 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P25635
#38: Protein
Budsiteselectionprotein21 / Bud21 / U three protein 16 / U3 small nucleolar RNA-associated protein 16 / U3 snoRNA-associated protein 16
Mass: 24431.016 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q08492
#44: Protein
Somethingaboutsilencingprotein10 / Sas10 / U three protein 3 / U3 small nucleolar RNA-associated protein 3 / U3 snoRNA-associated protein 11
Mass: 70364.398 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q12136
#45: Protein
ProteinSOF1 / Sof1 / U3 small nucleolar RNA-associated protein SOF1 / U3 snoRNA-associated protein SOF1
Mass: 56888.918 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P33750
#50: Protein
KRR1smallsubunitprocessomecomponent / / KRR-R motif-containing protein 1 / Ribosomal RNA assembly protein KRR1
Mass: 37226.254 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P25586
#51: Protein
rRNAbiogenesisproteinRRP5 / Ribosomal RNA-processing protein 5 / U3 small nucleolar RNA-associated protein RRP5 / U3 snoRNA- ...Ribosomal RNA-processing protein 5 / U3 small nucleolar RNA-associated protein RRP5 / U3 snoRNA-associated protein RRP5
Mass: 193411.422 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q05022
Mass: 119509.445 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c References: UniProt: P53914, Transferases; Acyltransferases; Transferring groups other than aminoacyl groups
#63: Protein
Essentialnuclearprotein1 / Enp1
Mass: 55207.422 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P38333
#64: Protein
Pno1 /
Mass: 36356.477 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae S288C (yeast)
#65: Protein
ProteinFAF1 / Forty S assembly factor
Mass: 38961.512 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P40546
#66: Protein
RegulatorofrDNAtranscriptionprotein14 / Rrt14
Mass: 23898.289 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P40470
#67: Protein
ProteinBFR2 / Brefeldin A resistance protein 2
Mass: 61285.039 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q06631
#68: Protein
Unassignedhelices
Mass: 29549.291 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
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Nucleolar protein ... , 2 types, 2 molecules 3D3E
#21: Protein
Nucleolarprotein56 / Nucleolus / Nop56 / Ribosome biosynthesis protein SIK1 / Suppressor of I kappa b protein 1
Mass: 56961.152 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q12460
#22: Protein
Nucleolarprotein58 / Nucleolus / Nucleolar protein 5
Mass: 57060.344 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q12499
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Ribosomal RNA-processing protein ... , 2 types, 2 molecules 3FRF
#23: Protein
RibosomalRNA-processingprotein9 / Rrp9
Mass: 65146.969 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q06506
#53: Protein
RibosomalRNA-processingprotein7
Mass: 34526.441 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P25368
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U3 small nucleolar RNA-associated protein ... , 17 types, 17 molecules A4A5A8A9AEAFB2B3B8BEB65C5D5ERERPRQ
#25: Protein
U3smallnucleolarRNA-associatedprotein4 / Utp4 / U3 snoRNA-associated protein 4 / U three protein 4 / U3 protein 4 required for transcription / t-UTP4
Mass: 87909.242 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q06679
#26: Protein
U3smallnucleolarRNA-associatedprotein5 / Utp5 / U3 snoRNA-associated protein 5 / U three protein 5 / U3 protein 5 required for transcription / t-UTP5
Mass: 72079.445 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q04177
#27: Protein
U3smallnucleolarRNA-associatedprotein8 / Utp8 / U3 snoRNA-associated protein 8 / U three protein 8 / U3 protein 8 required for transcription / t-UTP8
Mass: 80269.648 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P53276
#28: Protein
U3smallnucleolarRNA-associatedprotein9 / Utp9 / U3 snoRNA-associated protein 9 / U three protein 9 / U3 protein 9 required for transcription / t-UTP9
Mass: 65347.254 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P38882
#29: Protein
U3smallnucleolarRNA-associatedprotein10 / Utp10 / U3 snoRNA-associated protein 10 / U three protein 10 / U3 protein 10 required for ...Utp10 / U3 snoRNA-associated protein 10 / U three protein 10 / U3 protein 10 required for transcription / t-UTP10
Mass: 200298.984 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P42945
#30: Protein
U3smallnucleolarRNA-associatedprotein15 / Utp15 / U3 snoRNA-associated protein 15 / U three protein 15 / U3 protein 15 required for ...Utp15 / U3 snoRNA-associated protein 15 / U three protein 15 / U3 protein 15 required for transcription / t-UTP15
Mass: 57765.289 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q04305
#33: Protein
U3smallnucleolarRNA-associatedprotein12 / Utp12 / U3 snoRNA-associated protein 12 / DOM34-interacting protein 2 / U three protein 12
Mass: 106481.133 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q12220
#34: Protein
U3smallnucleolarRNA-associatedprotein13 / Utp13 / U3 snoRNA-associated protein 13 / U three protein 13
Mass: 91132.562 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q05946
#35: Protein
U3smallnucleolarRNA-associatedprotein18 / Utp18 / U3 snoRNA-associated protein 18 / U three protein 18
Mass: 66494.250 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P40362
#36: Protein
U3smallnucleolarRNA-associatedprotein21 / U3 snoRNA-associated protein 21 / U three protein 21
Mass: 104927.844 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q06078
#37: Protein
U3smallnucleolarRNA-associatedprotein6 / Utp6 / U3 snoRNA-associated protein 6 / U three protein 6
Mass: 52495.277 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q02354
#39: Protein
U3smallnucleolarRNA-associatedprotein7 / Utp7 / U3 snoRNA-associated protein 7 / U three protein 7
Mass: 62418.570 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P40055
#40: Protein
U3smallnucleolarRNA-associatedprotein11 / Utp11 / U3 snoRNA-associated protein 11 / U three protein 11
Mass: 29806.348 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P34247
#41: Protein
U3smallnucleolarRNA-associatedproteinMPP10 / Mpp10 / U3 snoRNA-associated protein MPP10 / M phase phosphoprotein 10
Mass: 67042.492 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P47083
#52: Protein
U3smallnucleolarRNA-associatedprotein22 / U3 snoRNA-associated protein 22 / U three protein 22
Mass: 140660.141 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P53254
#61: Protein
U3smallnucleolarRNA-associatedprotein20 / U3 snoRNA-associated protein 20 / U three protein 20
Mass: 287915.500 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P35194
#62: Protein
U3smallnucleolarRNA-associatedprotein14 / Utp14 / U3 snoRNA-associated protein 14 / U three protein 14
Mass: 103189.750 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q04500
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U3 small nucleolar ribonucleoprotein protein ... , 3 types, 3 molecules 5F5GRB
#42: Protein
U3smallnucleolarribonucleoproteinproteinIMP3 / Imp3 / U3 snoRNP protein IMP3 / Interacting with MPP10 protein 3
Mass: 21928.529 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P32899
#43: Protein
U3smallnucleolarribonucleoproteinproteinIMP4 / Imp4 / U3 snoRNP protein IMP4 / Interacting with MPP10 protein 4
Mass: 33536.168 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P53941
Electron dose: 50 e/Å2 / Detector mode: SUPER-RESOLUTION / Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Num. of grids imaged: 10 / Num. of real images: 18028
Image scans
Movie frames/image: 32 / Used frames/image: 1-32
-
Processing
EM software
ID
Name
Version
Category
2
SerialEM
imageacquisition
4
CTFFIND
4.1.8
CTFcorrection
7
UCSF Chimera
modelfitting
8
Coot
modelfitting
10
RELION
initialEulerassignment
11
RELION
finalEulerassignment
12
RELION
classification
13
RELION
3Dreconstruction
CTF correction
Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Particle selection
Num. of particles selected: 382298
Symmetry
Point symmetry: C1 (asymmetric)
3D reconstruction
Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 26582 / Symmetry type: POINT
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