+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 6kn7 | ||||||
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タイトル | Structure of human cardiac thin filament in the calcium free state | ||||||
要素 |
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キーワード | CONTRACTILE PROTEIN/ACTIN BINDING PROTEIN / Troponin (トロポニン) / Tropomyosin (トロポミオシン) / Actin (アクチン) / Thin filement / Muscle (骨格筋) / CONTRACTILE PROTEIN-ACTIN BINDING PROTEIN complex | ||||||
機能・相同性 | 機能・相同性情報 positive regulation of heart rate by epinephrine / muscle thin filament tropomyosin / regulation of systemic arterial blood pressure by ischemic conditions / troponin C binding / diaphragm contraction / regulation of ATP-dependent activity / regulation of muscle filament sliding speed / troponin T binding / cardiac Troponin complex / cardiac myofibril ...positive regulation of heart rate by epinephrine / muscle thin filament tropomyosin / regulation of systemic arterial blood pressure by ischemic conditions / troponin C binding / diaphragm contraction / regulation of ATP-dependent activity / regulation of muscle filament sliding speed / troponin T binding / cardiac Troponin complex / cardiac myofibril / regulation of smooth muscle contraction / トロポニン / bleb / negative regulation of vascular associated smooth muscle cell migration / regulation of muscle contraction / muscle filament sliding / transition between fast and slow fiber / negative regulation of ATP-dependent activity / ruffle organization / regulation of cardiac muscle contraction by calcium ion signaling / positive regulation of ATP-dependent activity / Striated Muscle Contraction / response to metal ion / regulation of heart contraction / structural constituent of muscle / sarcomere organization / cytoskeletal motor activator activity / ventricular cardiac muscle tissue morphogenesis / tropomyosin binding / heart contraction / myosin heavy chain binding / mesenchyme migration / troponin I binding / actin filament bundle / filamentous actin / negative regulation of vascular associated smooth muscle cell proliferation / actin filament bundle assembly / skeletal muscle thin filament assembly / striated muscle thin filament / skeletal muscle contraction / skeletal muscle myofibril / actin monomer binding / positive regulation of cell adhesion / Smooth Muscle Contraction / calcium channel inhibitor activity / 脈管形成 / skeletal muscle fiber development / stress fiber / cardiac muscle contraction / Ion homeostasis / titin binding / positive regulation of stress fiber assembly / cytoskeleton organization / actin filament polymerization / cytoskeletal protein binding / sarcomere / negative regulation of cell migration / filopodium / actin filament organization / マイクロフィラメント / 加水分解酵素; 酸無水物に作用; 酸無水物に作用・細胞または細胞小器官の運動に関与 / wound healing / structural constituent of cytoskeleton / intracellular calcium ion homeostasis / ruffle membrane / cellular response to reactive oxygen species / response to calcium ion / calcium-dependent protein binding / actin filament binding / マイクロフィラメント / lamellipodium / cell body / heart development / actin binding / regulation of cell shape / 細胞骨格 / hydrolase activity / protein heterodimerization activity / protein domain specific binding / calcium ion binding / positive regulation of gene expression / protein kinase binding / magnesium ion binding / protein homodimerization activity / ATP binding / identical protein binding / 細胞質基質 / 細胞質 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) Oryctolagus cuniculus (ウサギ) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 6.6 Å | ||||||
データ登録者 | Fujii, T. / Yamada, Y. / Namba, K. | ||||||
引用 | ジャーナル: Nat Commun / 年: 2020 タイトル: Cardiac muscle thin filament structures reveal calcium regulatory mechanism. 著者: Yurika Yamada / Keiichi Namba / Takashi Fujii / 要旨: Contraction of striated muscles is driven by cyclic interactions of myosin head projecting from the thick filament with actin filament and is regulated by Ca released from sarcoplasmic reticulum. ...Contraction of striated muscles is driven by cyclic interactions of myosin head projecting from the thick filament with actin filament and is regulated by Ca released from sarcoplasmic reticulum. Muscle thin filament consists of actin, tropomyosin and troponin, and Ca binding to troponin triggers conformational changes of troponin and tropomyosin to allow actin-myosin interactions. However, the structural changes involved in this regulatory mechanism remain unknown. Here we report the structures of human cardiac muscle thin filament in the absence and presence of Ca by electron cryomicroscopy. Molecular models in the two states built based on available crystal structures reveal the structures of a C-terminal region of troponin I and an N-terminal region of troponin T in complex with the head-to-tail junction of tropomyosin together with the troponin core on actin filament. Structural changes of the thin filament upon Ca binding now reveal the mechanism of Ca regulation of muscle contraction. | ||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | 分子: MolmilJmol/JSmol |
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 6kn7.cif.gz | 1.3 MB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb6kn7.ent.gz | 1 MB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 6kn7.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/kn/6kn7 ftp://data.pdbj.org/pub/pdb/validation_reports/kn/6kn7 | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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-要素
-タンパク質 , 4種, 21分子 ABCDEFGHIJKLMNOTaUbVc
#1: タンパク質 | 分子量: 41862.613 Da / 分子数: 15 / 由来タイプ: 天然 / 由来: (天然) Oryctolagus cuniculus (ウサギ) / 参照: UniProt: P68135 #4: タンパク質 | 分子量: 23023.039 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: TNNT2 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P45379 #5: タンパク質 | 分子量: 19639.691 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: TNNI3, TNNC1 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P19429 #6: タンパク質 | 分子量: 18288.287 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: TNNC1, TNNC / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P63316 |
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-Tropomyosin alpha-1 ... , 2種, 8分子 PQWXRSYZ
#2: タンパク質 | 分子量: 31555.053 Da / 分子数: 4 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: TPM1, C15orf13, TMSA / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P09493 #3: タンパク質・ペプチド | 分子量: 3528.104 Da / 分子数: 4 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: TPM1, C15orf13, TMSA / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P09493 |
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-非ポリマー , 1種, 15分子
#7: 化合物 | ChemComp-ADP / |
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-詳細
研究の焦点であるリガンドがあるか | N |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: FILAMENT / 3次元再構成法: 単粒子再構成法 |
-試料調製
構成要素 |
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由来(天然) |
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由来(組換発現) | 生物種: Escherichia coli (大腸菌) | ||||||||||||||||||||||||
緩衝液 | pH: 7.5 | ||||||||||||||||||||||||
試料 | 濃度: 0.05 mg/ml / 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES | ||||||||||||||||||||||||
急速凍結 | 凍結剤: ETHANE |
-電子顕微鏡撮影
顕微鏡 | モデル: JEOL CRYO ARM 200 |
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電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 200 kV / 照射モード: FLOOD BEAM |
電子レンズ | モード: BRIGHT FIELDBright-field microscopy |
撮影 | 電子線照射量: 65 e/Å2 フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) |
-解析
CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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3次元再構成 | 解像度: 6.6 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 21588 / 対称性のタイプ: POINT |