EMDB-7109: S. cerevisiae spliceosomal post-catalytic P complex

Basic information

• Entry: Database: EMDB / ID: EMD-7109
• Title: S. cerevisiae spliceosomal post-catalytic P complex
• Map data: Spliceosomal post-catalytic P complex

Sample

• Complex: Spliceosomal P complex
  • RNA: x 5 types
  • Protein or peptide: x 31 types
• Ligand: x 4 types

Function / homology

Function:

U2-type post-spliceosomal complex / U2-type post-mRNA release spliceosomal complex / spliceosomal complex disassembly / mRNA branch site recognition / cellular bud site selection / pre-mRNA 3'-splice site binding / post-mRNA release spliceosomal complex / generation of catalytic spliceosome for first transesterification step / nuclear mRNA surveillance / cis assembly of pre-catalytic spliceosome / splicing factor binding / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / U4/U6 snRNP / Prp19 complex / 7-methylguanosine cap hypermethylation / pICln-Sm protein complex / pre-mRNA binding / U2-type catalytic step 1 spliceosome / small nuclear ribonucleoprotein complex / SMN-Sm protein complex / spliceosomal tri-snRNP complex / U2-type spliceosomal complex / mRNA cis splicing, via spliceosome / commitment complex / U2-type prespliceosome assembly / U2-type catalytic step 2 spliceosome / poly(U) RNA binding / U4 snRNP / U2 snRNP / U1 snRNP / U2-type prespliceosome / precatalytic spliceosome / Formation of TC-NER Pre-Incision Complex / generation of catalytic spliceosome for second transesterification step / spliceosomal complex assembly / DNA replication origin binding / Gap-filling DNA repair synthesis and ligation in TC-NER / mRNA 5'-splice site recognition / regulation of RNA splicing / protein K63-linked ubiquitination / mRNA 3'-splice site recognition / Dual incision in TC-NER / spliceosomal tri-snRNP complex assembly / DNA replication initiation / U5 snRNA binding / U5 snRNP / U2 snRNA binding / U6 snRNA binding / spliceosomal snRNP assembly / positive regulation of cell cycle / pre-mRNA intronic binding / U1 snRNA binding / U4/U6 x U5 tri-snRNP complex / catalytic step 2 spliceosome / nuclear periphery / positive regulation of RNA splicing / RNA polymerase II transcription regulatory region sequence-specific DNA binding / spliceosomal complex / RING-type E3 ubiquitin transferase / mRNA splicing, via spliceosome / ubiquitin-protein transferase activity / metallopeptidase activity / ubiquitin protein ligase activity / RNA helicase activity / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA helicase / cell cycle / DNA repair / GTPase activity / mRNA binding / chromatin binding / chromatin / GTP binding / ATP hydrolysis activity / mitochondrion / RNA binding / zinc ion binding / ATP binding / identical protein binding / nucleus / metal ion binding / cytosol / cytoplasm

Domain/homology:

Prp18 / Pre-mRNA-splicing factor 18 / Prp18 domain / Pre-mRNA-splicing factor SLU7 domain / Pre-mRNA-splicing factor SLU7 / Pre-mRNA splicing Prp18-interacting factor / DHX8/ Prp22, DEXH-box helicase domain / : / : / Slt11, RNA recognition motif / cwf21 / Torus domain / Pre-mRNA-splicing factor Cwc2, RNA recognition motif / Torus domain / mRNA splicing factor SYF2 / SYF2 splicing factor / mRNA splicing factor Cwf21 domain / cwf21 domain / Pre-mRNA-processing factor 17 / : / STL11, N-terminal / RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) / Pre-mRNA-splicing factor 19 / Pre-mRNA-processing factor 19 / Prp19/Pso4-like / WD repeat Prp46/PLRG1-like / BUD31/G10-related, conserved site / : / : / G10 protein signature 1. / G10 protein signature 2. / SKI-interacting protein SKIP, SNW domain / SKI-interacting protein, SKIP / SKIP/SNW domain / Pre-mRNA-splicing factor Cwf15/Cwc15 / HAT (Half-A-TPR) repeat / Cwf15/Cwc15 cell cycle control protein / Pre-mRNA-splicing factor Cwc2/Slt11 / G10 protein / Pre-mRNA-splicing factor BUD31 / Pre-mRNA splicing factor component Cdc5p/Cef1, C-terminal / pre-mRNA splicing factor component / Initiation factor eIF-4 gamma, MA3 / MA3 domain / MI domain profile. / Domain in DAP-5, eIF4G, MA-3 and other proteins. / U-box domain profile. / Middle domain of eukaryotic initiation factor 4G (eIF4G) / MIF4G-like, type 3 / Modified RING finger domain / U-box domain / U2A'/phosphoprotein 32 family A, C-terminal / occurring C-terminal to leucine-rich repeats / Leucine-rich repeat / : / Helicase associated domain (HA2), ratchet-like / Pre-mRNA-splicing factor Syf1-like / Snu114, GTP-binding domain / DEAD-box helicase, OB fold / Oligonucleotide/oligosaccharide-binding (OB)-fold / Helicase-associated domain / Helicase associated domain (HA2), winged-helix / Helicase associated domain (HA2) Add an annotation / 116kDa U5 small nuclear ribonucleoprotein component, N-terminal / 116kDa U5 small nuclear ribonucleoprotein component, C-terminal / 116 kDa U5 small nuclear ribonucleoprotein component N-terminus / Small nuclear ribonucleoprotein Sm D3 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein F / Sm-like protein Lsm7/SmG / Myb-type HTH DNA-binding domain profile. / Like-Sm (LSM) domain containing protein, LSm4/SmD1/SmD3 / Zinc finger, CCCH-type / Zinc finger C3H1-type profile. / Sm-like protein Lsm6/SmF / LSM domain / LSM domain, eukaryotic/archaea-type / snRNP Sm proteins / HAT (Half-A-TPR) repeat / HAT (Half-A-TPR) repeats / Myb domain / : / Sm domain profile. / DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site / DEAH-box subfamily ATP-dependent helicases signature. / Myb-like DNA-binding domain / LSM domain superfamily / Translation elongation factor EFG/EF2, domain IV / Elongation factor G, domain IV / Elongation factor G, domain IV / Elongation factor G C-terminus / Quinoprotein alcohol dehydrogenase-like superfamily / Elongation factor EFG, domain V-like / Elongation factor G C-terminus / EF-G domain III/V-like / SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains / SANT/Myb domain / S1 domain profile.

Component:

Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22 / Pre-mRNA-splicing factor BUD31 / Pre-mRNA-processing protein 45 / Pre-mRNA-processing factor 19 / Pre-mRNA-splicing factor 8 / Pre-mRNA-splicing factor 18 / Pre-mRNA-splicing factor SNU114 / Pre-mRNA-splicing factor SLT11 / Small nuclear ribonucleoprotein-associated protein B / Small nuclear ribonucleoprotein G / U2 small nuclear ribonucleoprotein B'' / Pre-mRNA-processing factor 17 / Small nuclear ribonucleoprotein Sm D3 / Pre-mRNA-splicing factor SYF2 / Pre-mRNA-splicing factor CWC22 / Small nuclear ribonucleoprotein F / Small nuclear ribonucleoprotein Sm D1 / Pre-mRNA-splicing factor SLU7 / Pre-mRNA-splicing factor CWC21 / Pre-mRNA-splicing factor CEF1 / Pre-mRNA-splicing factor CWC15 / Pre-mRNA-splicing factor SYF1 / Pre-mRNA-splicing factor SNT309 / Small nuclear ribonucleoprotein Sm D2 / U2 small nuclear ribonucleoprotein A' / Pre-mRNA-splicing factor CWC2 / Pre-mRNA-splicing factor CLF1 / Small nuclear ribonucleoprotein E / Pre-mRNA-splicing factor PRP46

• Biological species: Saccharomyces cerevisiae (brewer's yeast) / Baker's yeast (brewer's yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 3.3 Å
• Authors: Liu S / Li X / Zhou ZH / Zhao R

Funding support

United States, 3 items

Organization	Grant number	Country
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	GM114178	United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)	AI094386	United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	GM071940	United States

• Citation: Journal: Science / Year: 2017; Title: Structure of the yeast spliceosomal postcatalytic P complex.; Authors: Shiheng Liu / Xueni Li / Lingdi Zhang / Jiansen Jiang / Ryan C Hill / Yanxiang Cui / Kirk C Hansen / Z Hong Zhou / Rui Zhao / ; Abstract: The spliceosome undergoes dramatic changes in a splicing cycle. Structures of B, B, C, C*, and intron lariat spliceosome complexes revealed mechanisms of 5'-splice site (ss) recognition, branching, and intron release, but lacked information on 3'-ss recognition, exon ligation, and exon release. Here we report a cryo-electron microscopy structure of the postcatalytic P complex at 3.3-angstrom resolution, revealing that the 3' ss is mainly recognized through non-Watson-Crick base pairing with the 5' ss and branch point. Furthermore, one or more unidentified proteins become stably associated with the P complex, securing the 3' exon and potentially regulating activity of the helicase Prp22. Prp22 binds nucleotides 15 to 21 in the 3' exon, enabling it to pull the intron-exon or ligated exons in a 3' to 5' direction to achieve 3'-ss proofreading or exon release, respectively.

History

Deposition	Nov 7, 2017	-
Header (metadata) release	Feb 21, 2018	-
Map release	Feb 21, 2018	-
Update	Oct 14, 2020	-
Current status	Oct 14, 2020	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_7109.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Spliceosomal post-catalytic P complex
• Voxel size: X=Y=Z: 1.36 Å

Density

Contour Level	By AUTHOR: 0.06 / Movie #1: 0.06
Minimum - Maximum	-0.19713637 - 0.40362555
Average (Standard dev.)	-0.00004110106 (±0.007809853)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 400 400 400 Spacing 400 400 400
Cell	A=B=C: 544.0 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.36	1.36	1.36
M x/y/z	400	400	400
origin x/y/z	0.000	0.000	0.000
length x/y/z	544.000	544.000	544.000
α/β/γ	90.000	90.000	90.000
start NX/NY/NZ	0	0	0
NX/NY/NZ	22	44	128
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	400	400	400
D min/max/mean	-0.197	0.404	-0.000

Supplemental data

Sample components

Entire : Spliceosomal P complex

• Entire: Name: Spliceosomal P complex

Components

• Complex: Spliceosomal P complex
  • RNA: U2 snRNAU2 spliceosomal RNA
  • RNA: U5 snRNAU5 spliceosomal RNA
  • RNA: U6 snRNAU6 spliceosomal RNA
  • RNA: RNA (34-MER)
  • RNA: RNA (59-MER)
  • Protein or peptide: Pre-mRNA-splicing factor Prp8
  • Protein or peptide: Pre-mRNA-splicing factor SNU114
  • Protein or peptide: Pre-mRNA-splicing factor PRP46
  • Protein or peptide: Pre-mRNA-processing protein 45
  • Protein or peptide: Pre-mRNA-splicing factor SLT11
  • Protein or peptide: Pre-mRNA-splicing factor CWC2
  • Protein or peptide: Pre-mRNA-splicing factor CWC15
  • Protein or peptide: Pre-mRNA-splicing factor BUD31
  • Protein or peptide: Pre-mRNA-splicing factor CWC21
  • Protein or peptide: Pre-mRNA-splicing factor CWC22
  • Protein or peptide: Pre-mRNA-processing factor Prp17
  • Protein or peptide: Pre-mRNA-splicing factor Prp18
  • Protein or peptide: Pre-mRNA-splicing factor SLU7
  • Protein or peptide: Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22
  • Protein or peptide: Pre-mRNA-splicing factor SYF2
  • Protein or peptide: Pre-mRNA-splicing factor CEF1
  • Protein or peptide: Pre-mRNA-splicing factor CLF1
  • Protein or peptide: Pre-mRNA-splicing factor SYF1
  • Protein or peptide: Unknown protein fragment
  • Protein or peptide: Unknown protein fragment
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D2
  • Protein or peptide: Small nuclear ribonucleoprotein F
  • Protein or peptide: Small nuclear ribonucleoprotein E
  • Protein or peptide: Small nuclear ribonucleoprotein G
  • Protein or peptide: Small nuclear ribonucleoprotein-associated protein B
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D3
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D1
  • Protein or peptide: Lea1
  • Protein or peptide: Msl1
  • Protein or peptide: Pre-mRNA-processing factor Prp19
  • Protein or peptide: Pre-mRNA-splicing factor SNT309
• Ligand: MAGNESIUM ION
• Ligand: INOSITOL HEXAKISPHOSPHATEPhytic acid
• Ligand: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate
• Ligand: ZINC ION

Supramolecule #1: Spliceosomal P complex

• Supramolecule: Name: Spliceosomal P complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#36
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)

Macromolecule #1: U2 snRNA

• Macromolecule: Name: U2 snRNA / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 376.267406 KDa

Sequence

String:

ACGAAUCUCU UUGCCUUUUG GCUUAGAUCA AGUGUAGUAU CUGUUCUUUU CAGUGUAACA ACUGAAAUGA CCUCAAUGAG GCUCAUUAC CUUUUAAUUU GUUACAAUAC ACAUUUUUUG GCACCCAAAA UAAUAAAAUG GACGGGAAGA GACUUUUUAA G CAAGUUGU UUUCCGCUAA UGUCAGGUCU CACUACUUUU UGCUGCUAUU UUUCUUCGCU CAUGGUUUCU UCAUAAGGCG UU UUUAUGA UGGUUUUUCG AAAUUGGUUU UUGAGACGAC GGUUGCUCAA GGUUAUUGUU UUUGUUUUCU UCUGGUUGUU UUC UAUUUU CUUUUUUUUA GCUUUCUGUU UCUCCCUUAG UUUGGCUUUU UGCUUCAUAC UCUUCCCUGU CUUUCCGAGC CGUU UAUGU CCAACGCGGG AUUUGGUUUU UCUUUAUCGA UGGGAAGAAA UGGUGCUAUA GUAGGUUGGG AGAUAAUAUU UAUGG UAUG GGGUGCUAGU GCGGAUGGGG CGCUCUUAUU GUUGAUUUCU UCGCUCGUCU UCUUUUUCUG GUGGCGCUGC AAGAGG AAG UUUUUCGACU UUGUUAUGAU UUUUGGUUUG CAAGGAAAGG UGUCUUACGA UUCUUUUUUU GAUGUAAUAG GAUAAGC UU GCUUAUCCCC CAAGUAUCGG CCAAAGUUGU UGAUUUUCCU UUUGAAGUGU CCUCGGUUUG AGGGGGUGUA GGGUGGGG U UGGUCUACAA UAAGAGUGUU CCAUUGUUAA CGUGCUGGCG UCUUUUACUA UAUUUUUUUU CCCAGUUUAU UUUGUGCUU AUUUUCUCAU UGAGGAGAAG GAGCUCUUCU CGCAGGAUAU AAAUGGAGGU UUGCUAAAGG GGAGGAGAUG UGUUUGUGAG AAUACUGCU GAGAGAGUUC UGGAAGAGAA AAAAAGGAGG CAAUGGAAGG CGUUUGCUGG GAAAAGAGAA GAGCCAUGAC U GCAUCUGU UGUUUCAAGG CCAGUUUUAU UAACCGCCUA UGUCAUAGAG GCGUUUUUUU UGGAGGGAUU UGAAGAAUGC CG GCGGCAU CAAGAAACGG ACUUGAUGGU UGACGCCUGU UUUUAAAGUU AGAGACGUCG CGACCCUCGC ACUUGUGGAG UCG UUCUUG ACUUUUACUU UGGUCGCUUG AUGUUUCUCU CGUCUUCCCG UUCGCUCUU

Macromolecule #2: U5 snRNA

• Macromolecule: Name: U5 snRNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 68.643344 KDa

Sequence

String:

AAGCAGCUUU ACAGAUCAAU GGCGGAGGGA GGUCAACAUC AAGAACUGUG GGCCUUUUAU UGCCUAUAGA ACUUAUAACG AACAUGGUU CUUGCCUUUU ACCAGAACCA UCCGGGUGUU GUCUCCAUAG AAACAGGUAA AGCUGUCCGU UACUGUGGGC U UGCCAUAU UUUUUGGAAC UUUUCUGCCC UUUUUCUCAA UGAGUAAGGA GGGCGU

Macromolecule #3: U6 snRNA

• Macromolecule: Name: U6 snRNA / type: rna / ID: 3 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 35.883176 KDa

Sequence

String:

GUUCGCGAAG UAACCCUUCG UGGACAUUUG GUCAAUUUGA AACAAUACAG AGAUGAUCAG CAGUUCCCCU GCAUAAGGAU GAACCGUUU UACAAAGAGA UUUAUUUCGU UUU

Macromolecule #4: RNA (34-MER)

• Macromolecule: Name: RNA (34-MER) / type: rna / ID: 4 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 10.680198 KDa

Sequence

String:

UUUAAUAAAA AAAGUAUAUU UUUUUUUUUU UUAU

Macromolecule #5: RNA (59-MER)

• Macromolecule: Name: RNA (59-MER) / type: rna / ID: 5 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 18.717949 KDa

Sequence

String:

GUAUGUAUUU AUUUUAGAAC UAGUUACUAA CAUUUUUUUU UUAAAAAAUA AUUAUAUAG

Macromolecule #6: Pre-mRNA-splicing factor Prp8

• Macromolecule: Name: Pre-mRNA-splicing factor Prp8 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 279.867469 KDa

Sequence

String:

MSGLPPPPPG FEEDSDLALP PPPPPPPGYE IEELDNPMVP SSVNEDTFLP PPPPPPSNFE INAEEIVDFT LPPPPPPPGL DELETKAEK KVELHGKRKL DIGKDTFVTR KSRKRAKKMT KKAKRSNLYT PKAEMPPEHL RKIINTHSDM ASKMYNTDKK A FLGALKYL PHAILKLLEN MPHPWEQAKE VKVLYHTSGA ITFVNETPRV IEPVYTAQWS ATWIAMRREK RDRTHFKRMR FP PFDDDEP PLSYEQHIEN IEPLDPINLP LDSQDDEYVK DWLYDSRPLE EDSKKVNGTS YKKWSFDLPE MSNLYRLSTP LRD EVTDKN YYYLFDKKSF FNGKALNNAI PGGPKFEPLY PREEEEDYNE FNSIDRVIFR VPIRSEYKVA FPHLYNSRPR SVRI PWYNN PVSCIIQNDE EYDTPALFFD PSLNPIPHFI DNNSSLNVSN TKENGDFTLP EDFAPLLAEE EELILPNTKD AMSLY HSPF PFNRTKGKMV RAQDVALAKK WFLQHPDEEY PVKVKVSYQK LLKNYVLNEL HPTLPTNHNK TKLLKSLKNT KYFQQT TID WVEAGLQLCR QGHNMLNLLI HRKGLTYLHL DYNFNLKPTK TLTTKERKKS RLGNSFHLMR ELLKMMKLIV DTHVQFR LG NVDAFQLADG IHYILNHIGQ LTGIYRYKYK VMHQIRACKD LKHIIYYKFN KNLGKGPGCG FWQPAWRVWL NFLRGTIP L LERYIGNLIT RQFEGRSNEI VKTTTKQRLD AYYDLELRNS VMDDILEMMP ESIRQKKART ILQHLSEAWR CWKANIPWD VPGMPAPIKK IIERYIKSKA DAWVSAAHYN RERIKRGAHV EKTMVKKNLG RLTRLWIKNE QERQRQIQKN GPEITPEEAT TIFSVMVEW LESRSFSPIP FPPLTYKNDT KILVLALEDL KDVYASKVRL NASEREELAL IEEAYDNPHD TLNRIKKYLL T QRVFKPVD ITMMENYQNI SPVYSVDPLE KITDAYLDQY LWYEADQRKL FPNWIKPSDS EIPPLLVYKW TQGINNLSEI WD VSRGQSA VLLETTLGEM AEKIDFTLLN RLLRLIVDPN IADYITAKNN VVINFKDMSH VNKYGLIRGL KFASFIFQYY GLV IDLLLL GQERATDLAG PANNPNEFMQ FKSKEVEKAH PIRLYTRYLD RIYMLFHFEE DEGEELTDEY LAENPDPNFE NSIG YNNRK CWPKDSRMRL IRQDVNLGRA VFWEIQSRVP TSLTSIKWEN AFVSVYSKNN PNLLFSMCGF EVRILPRQRM EEVVS NDEG VWDLVDERTK QRTAKAYLKV SEEEIKKFDS RIRGILMASG STTFTKVAAK WNTSLISLFT YFREAIVATE PLLDIL VKG ETRIQNRVKL GLNSKMPTRF PPAVFYTPKE LGGLGMISAS HILIPASDLS WSKQTDTGIT HFRAGMTHED EKLIPTI FR YITTWENEFL DSQRVWAEYA TKRQEAIQQN RRLAFEELEG SWDRGIPRIS TLFQRDRHTL AYDRGHRIRR EFKQYSLE R NSPFWWTNSH HDGKLWNLNA YRTDVIQALG GIETILEHTL FKGTGFNSWE GLFWEKASGF EDSMQFKKLT HAQRTGLSQ IPNRRFTLWW SPTINRANVY VGFLVQLDLT GIFLHGKIPT LKISLIQIFR AHLWQKIHES IVFDICQILD GELDVLQIES VTKETVHPR KSYKMNSSAA DITMESVHEW EVSKPSLLHE TNDSFKGLIT NKMWFDVQLR YGDYDSHDIS RYVRAKFLDY T TDNVSMYP SPTGVMIGID LAYNMYDAYG NWFNGLKPLI QNSMRTIMKA NPALYVLRER IRKGLQIYQS SVQEPFLNSS NY AELFNND IKLFVDDTNV YRVTVHKTFE GNVATKAING CIFTLNPKTG HLFLKIIHTS VWAGQKRLSQ LAKWKTAEEV SAL VRSLPK EEQPKQIIVT RKAMLDPLEV HMLDFPNIAI RPTELRLPFS AAMSIDKLSD VVMKATEPQM VLFNIYDDWL DRIS SYTAF SRLTLLLRAL KTNEESAKMI LLSDPTITIK SYHLWPSFTD EQWITIESQM RDLILTEYGR KYNVNISALT QTEIK DIIL GQNIKAPSVK RQKMAELEAA RSEKQNDEEA AGASTVMKTK TINAQGEEIV VVASADYESQ TFSSKNEWRK SAIANT LLY LRLKNIYVSA DDFVEEQNVY VLPKNLLKKF IEISDVKIQV AAFIYGMSAK DHPKVKEIKT VVLVPQLGHV GSVQISN IP DIGDLPDTEG LELLGWIHTQ TEELKFMAAS EVATHSKLFA DKKRDCIDIS IFSTPGSVSL SAYNLTDEGY QWGEENKD I MNVLSEGFEP TFSTHAQLLL SDRITGNFII PSGNVWNYTF MGTAFNQEGD YNFKYGIPLE FYNEMHRPVH FLQFSELAG DEELEAEQID VFS

Macromolecule #7: Pre-mRNA-splicing factor SNU114

• Macromolecule: Name: Pre-mRNA-splicing factor SNU114 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 114.174008 KDa

Sequence

String:

MEGDDLFDEF GNLIGVDPFD SDEEESVLDE QEQYQTNTFE GSGNNNEIES RQLTSLGSKK ELGISLEHPY GKEVEVLMET KNTQSPQTP LVEPVTERTK LQEHTIFTQL KKNIPKTRYN RDYMLSMANI PERIINVGVI GPLHSGKTSL MDLLVIDSHK R IPDMSKNV ELGWKPLRYL DNLKQEIDRG LSIKLNGSTL LCTDLESKSR MINFLDAPGH VNFMDETAVA LAASDLVLIV ID VVEGVTF VVEQLIKQSI KNNVAMCFVI NKLDRLILDL KLPPMDAYLK LNHIIANINS FTKGNVFSPI DNNIIFASTK LGF TFTIKE FVSYYYAHSI PSSKIDDFTT RLWGSVYYHK GNFRTKPFEN VEKYPTFVEF ILIPLYKIFS YALSMEKDKL KNLL RSNFR VNLSQEALQY DPQPFLKHVL QLIFRQQTGL VDAITRCYQP FELFDNKTAH LSIPGKSTPE GTLWAHVLKT VDYGG AEWS LVRIYSGLLK RGDTVRILDT SQSESRQKRQ LHDISKTETS NEDEDEDDET PSCEVEEIGL LGGRYVYPVH EAHKGQ IVL IKGISSAYIK SATLYSVKSK EDMKQLKFFK PLDYITEAVF KIVLQPLLPR ELPKLLDALN KISKYYPGVI IKVEESG EH VILGNGELYM DCLLYDLRAS YAKIEIKISD PLTVFSESCS NESFASIPVS NSISRLGEEN LPGLSISVAA EPMDSKMI Q DLSRNTLGKG QNCLDIDGIM DNPRKLSKIL RTEYGWDSLA SRNVWSFYNG NVLINDTLPD EISPELLSKY KEQIIQGFY WAVKEGPLAE EPIYGVQYKL LSISVPSDVN IDVMKSQIIP LMKKACYVGL LTAIPILLEP IYEVDITVHA PLLPIVEELM KKRRGSRIY KTIKVAGTPL LEVRGQVPVI ESAGFETDLR LSTNGLGMCQ LYFWHKIWRK VPGDVLDKDA FIPKLKPAPI N SLSRDFVM KTRRRKGIST GGFMSNDGPT LEKYISAELY AQLRENGLVP

Macromolecule #8: Pre-mRNA-splicing factor PRP46

• Macromolecule: Name: Pre-mRNA-splicing factor PRP46 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 50.771289 KDa

Sequence

String:

MDGNDHKVEN LGDVDKFYSR IRWNNQFSYM ATLPPHLQSE MEGQKSLLMR YDTYRKESSS FSGEGKKVTL QHVPTDFSEA SQAVISKKD HDTHASAFVN KIFQPEVAEE LIVNRYEKLL SQRPEWHAPW KLSRVINGHL GWVRCVAIDP VDNEWFITGS N DTTMKVWD LATGKLKTTL AGHVMTVRDV AVSDRHPYLF SVSEDKTVKC WDLEKNQIIR DYYGHLSGVR TVSIHPTLDL IA TAGRDSV IKLWDMRTRI PVITLVGHKG PINQVQCTPV DPQVVSSSTD ATVRLWDVVA GKTMKVLTHH KRSVRATALH PKE FSVASA CTDDIRSWGL AEGSLLTNFE SEKTGIINTL SINQDDVLFA GGDNGVLSFY DYKSGHKYQS LATREMVGSL EGER SVLCS TFDKTGLRLI TGEADKSIKI WKQDETATKE SEPGLAWNPN LSAKRF

Macromolecule #9: Pre-mRNA-processing protein 45

• Macromolecule: Name: Pre-mRNA-processing protein 45 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 42.548727 KDa

Sequence

String:

MFSNRLPPPK HSQGRVSTAL SSDRVEPAIL TDQIAKNVKL DDFIPKRQSN FELSVPLPTK AEIQECTART KSYIQRLVNA KLANSNNRA SSRYVTETHQ APANLLLNNS HHIEVVSKQM DPLLPRFVGK KARKVVAPTE NDEVVPVLHM DGSNDRGEAD P NEWKIPAA VSNWKNPNGY TVALERRVGK ALDNENNTIN DGFMKLSEAL ENADKKARQE IRSKMELKRL AMEQEMLAKE SK LKELSQR ARYHNGTPQT GAIVKPKKQT STVARLKELA YSQGRDVSEK IILGAAKRSE QPDLQYDSRF FTRGANASAK RHE DQVYDN PLFVQQDIES IYKTNYEKLD EAVNVKSEGA SGSHGPIQFT KAESDDKSDN YGA

Macromolecule #10: Pre-mRNA-splicing factor SLT11

• Macromolecule: Name: Pre-mRNA-splicing factor SLT11 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 40.98859 KDa

Sequence

String:

MNDEINEPPP NICEQCLGDE ANIRMTKIPQ GSECKICTLP FTLYHFKTSK RSNNIIKTLI CVRCATQRNI CQCCMLDSRW HIPIQLRDH LISLVNEENV MTEEAKNDMM KRFLSLKNVK LGGAQITSDP SEADNIVDKL KNILLRATSD GPSTPLIKNT T ALYKNEKG ANEVKNLEKY ASVDISHILK KLPLNESFLK NPSTKSFFLY NIDASIPEWK ITDTVSQLLG IKKWKDGNSL SL IVNHKAK CGGLRFQSSE LGERFVSKIS ETLVTPKGLK RGVLLIDRFR IFIIPWSSGF SAASFGTNTA ENIKLSLSLN KLI QLELGL SFPTKSTDNA KNDKKKTSKK VHKDRSKKSK PRANKLTI

Macromolecule #11: Pre-mRNA-splicing factor CWC2

• Macromolecule: Name: Pre-mRNA-splicing factor CWC2 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 38.486562 KDa

Sequence

String:

MTSWRDKSAK VQVKESELPS SIPAQTGLTF NIWYNKWSQG FAGNTRFVSP FALQPQLHSG KTRGDNDGQL FFCLFFAKGM CCLGPKCEY LHHIPDEEDI GKLALRTEVL DCFGREKFAD YREDMGGIGS FRKKNKTLYV GGIDGALNSK HLKPAQIESR I RFVFSRLG DIDRIRYVES KNCGFVKFKY QANAEFAKEA MSNQTLLLPS DKEWDDRREG TGLLVKWANE DPDPAAQKRL QE ELKLESL NMMVHLINNN TNSAGTEVNN KNNERLDRTF PEASVDNVKK RLLPLDNGME SDDFIEKLKK VKKNISRENI SSK PSVGKL GGPLLDYLSS DED

Macromolecule #12: Pre-mRNA-splicing factor CWC15

• Macromolecule: Name: Pre-mRNA-splicing factor CWC15 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 19.975195 KDa

Sequence

String:

MTTSHRPQLE ARSGAKAAAY TPTGIEHARL LPGHTTLKYR KFKEEENLRA NCAQEDRSND KSLEEAVMNE EKQDVVGSGN LQETRSEKD QKDSLQELLV TQKNKVEDKA ELEGNEQLKG GNSSRRSWRK GTAFGRHKVT KETNIKEHAT KKSASGYIND M TKSEYHQE FLHKHVR

Macromolecule #13: Pre-mRNA-splicing factor BUD31

• Macromolecule: Name: Pre-mRNA-splicing factor BUD31 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 18.484502 KDa

Sequence

String:

MPRIKTRRSK PAPDGFEKIK PTLTDFEIQL RDAQKDKSSK LAAKSNEQLW EIMQLHHQRS RYIYTLYYKR KAISKDLYDW LIKEKYADK LLIAKWRKTG YEKLCCLRCI QKNETNNGST CICRVPRAQL EEEARKKGTQ VSFHQCVHCG CRGCASTD

Macromolecule #14: Pre-mRNA-splicing factor CWC21

• Macromolecule: Name: Pre-mRNA-splicing factor CWC21 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.793596 KDa

Sequence

String:

MSYNGIGLKS AKGSSTSGHV QRSLASNNRR RPQGSQQQRQ QRQNAIKKAS HDKASRPLAV QKQIETHMEK REIEVQVSEL RDRLEEEET LSEEQIDKKC EALRAKLTNE WQEQQRMSSL YTPRKARLTE EQHRHE

Macromolecule #15: Pre-mRNA-splicing factor CWC22

• Macromolecule: Name: Pre-mRNA-splicing factor CWC22 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 67.386062 KDa

Sequence

String:

MSTATIQDED IKFQRENWEM IRSHVSPIIS NLTMDNLQES HRDLFQVNIL IGRNIICKNV VDFTLNKQNG RLIPALSALI ALLNSDIPD IGETLAKELM LMFVQQFNRK DYVSCGNILQ CLSILFLYDV IHEIVILQIL LLLLEKNSLR LVIAVMKICG W KLALVSKK THDMIWEKLR YILQTQELSS TLRESLETLF EIRQKDYKSG SQGLFILDPT SYTVHTHSYI VSDEDEANKE LG NFEKCEN FNELTMAFDT LRQKLLINNT SDTNEGSNSQ LQIYDMTSTN DVEFKKKIYL VLKSSLSGDE AAHKLLKLKI ANN LKKSVV DIIIKSSLQE STFSKFYSIL SERMITFHRS WQTAYNETFE QNYTQDIEDY ETDQLRILGK FWGHLISYEF LPMD CLKII KLTEEESCPQ GRIFIKFLFQ ELVNELGLDE LQLRLNSSKL DGMFPLEGDA EHIRYSINFF TAIGLGLLTE DMRSR LTII QEVEDAEEEE KKLREEEELE KLRKKARESQ PTQGPKIHES RLFLQKDTRE NSRSRSPFTV ETRKRARSRT PPRGSR NHR NRSRTPPARR QRHR

Macromolecule #16: Pre-mRNA-processing factor Prp17

• Macromolecule: Name: Pre-mRNA-processing factor Prp17 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 52.128762 KDa

Sequence

String:

MGLVDGYDTS SDSDLNFDEG KSVHEKKNGN LHEDTSYEPS SNNIHKRKSH FTKSELKRRR KTRKGDGPWG SWSSSDDETS QASETQKED QDIFVHALAE DNLDSEQIEV EEVSHFYGKS EKDYQGRGYL YPPNDVDVDL REERISFRCY LPKKVIRNYP G HPEGTTAL KFLPKTGHLI LSGGNDHTIK IWDFYHDYEC LRDFQGHNKP IKALRFTEDC QSFLSSSFDR SVKIWDTETG KV KTRLHLN STPADVESRP TNPHEFIVGL SNSKILHYDD RVSENQGLVQ TYDHHLSSIL ALKYFPDGSK FISSSEDKTV RIW ENQINV PIKQISDTAQ HSMPFLNVHP SQNYFCAQSM DNRIYSFSLK PKYKRHPKKI FKGHSSAGYG ISLAFSGDGR YICS GDSKS RLFTWDWNTS RLLNNIKIPG NKPITQVDWH PQETSKVICS GAAGKIYVCD

Macromolecule #17: Pre-mRNA-splicing factor Prp18

• Macromolecule: Name: Pre-mRNA-splicing factor Prp18 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 28.414391 KDa

Sequence

String:

MDLDLASILK GEISKKKKEL ANSKGVQPPC TEKFQPHESA NIDETPRQVE QESTDEENLS DNQSDDIRTT ISKLENRPER IQEAIAQDK TISVIIDPSQ IGSTEGKPLL SMKCNLYIHE ILSRWKASLE AYHPELFLDT KKALFPLLLQ LRRNQLAPDL L ISLATVLY HLQQPKEINL AVQSYMKLSI GNVAWPIGVT SVGIHARSAH SKIQGGRNAA NIMIDERTRL WITSIKRLIT FE EWYTSNH DSLA

Macromolecule #18: Pre-mRNA-splicing factor SLU7

• Macromolecule: Name: Pre-mRNA-splicing factor SLU7 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 44.722875 KDa

Sequence

String:

MNNNSRNNEN RSTINRNKRQ LQQAKEKNEN IHIPRYIRNQ PWYYKDTPKE QEGKKPGNDD TSTAEGGEKS DYLVHHRQKA KGGALDIDN NSEPKIGMGI KDEFKLIRPQ KMSVRDSHSL SFCRNCGEAG HKEKDCMEKP RKMQKLVPDL NSQKNNGTVL V RATDDDWD SRKDRWYGYS GKEYNELISK WERDKRNKIK GKDKSQTDET LWDTDEEIEL MKLELYKDSV GSLKKDDADN SQ LYRTSTR LREDKAAYLN DINSTESNYD PKSRLYKTET LGAVDEKSKM FRRHLTGEGL KLNELNQFAR SHAKEMGIRD EIE DKEKVQ HVLVANPTKY EYLKKKREQE ETKQPKIVSI GDLEARKVDG TKQSEEQRNH LKDLYG

Macromolecule #19: Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22

• Macromolecule: Name: Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22; type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 130.187359 KDa

Sequence

String:

MSDISKLIGA IVGSDDPVII EFVLNIINKS GNLQEFIRNI QKLDAGISYE DSIKMYNAFL GKQEEEKVRN KVKSSPLSQK INQVLKDDV NLDDPVVTEF VLSILNKSKS ITEFQEQLNL MQSGLDNETI FKIYQIASPP VMKEEVSVLP STKIPAKIEA K IEEEVQKI ESLDPSPVLH KVYEGKVRNI TTFGCFVQIF GTRMKNCDGL VHISEMSDQR TLDPHDVVRQ GQHIFVEVIK IQ NNGKISL SMKNIDQHSG EIRKRNTESV EDRGRSNDAH TSRNMKNKIK RRALTSPERW EIRQLIASGA ASIDDYPELK DEI PINTSY LTAKRDDGSI VNGNTEKVDS KLEEQQRDET DEIDVELNTD DGPKFLKDQQ VKGAKKYEMP KITKVPRGFM NRSA INGSN AIRDHREEKL RKKREIEQQI RKQQSFDDPT KNKKDSRNEI QMLKNQLIVT EWEKNRMNES ISYGKRTSLP ISAQR QTLP VYAMRSELIQ AVRDNQFLVI VGETGSGKTT QITQYLDEEG FSNYGMIGCT QPRRVAAVSV AKRVAEEVGC KVGHDV GYT IRFEDVTGPD TRIKYMTDGM LQREALLDPE MSKYSVIMLD EAHERTVATD VLFALLKKAA IKRPELKVIV TSATLNS AK FSEYFLNCPI INIPGKTFPV EVLYSQTPQM DYIEAALDCV IDIHINEGPG DILVFLTGQE EIDSCCEILY DRVKTLGD S IGELLILPVY SALPSEIQSK IFEPTPKGSR KVVFATNIAE TSITIDGIYY VVDPGFAKIN IYNARAGIEQ LIVSPISQA QANQRKGRAG RTGPGKCYRL YTESAFYNEM LENTVPEIQR QNLSHTILML KAMGINDLLK FDFMDPPPKN LMLNALTELY HLQSLDDEG KLTNLGKEMS LFPMDPTLSR SLLSSVDNQC SDEIVTIISM LSVQNVFYRP KDRQLEADSK KAKFHHPYGD H LTLLNVYT RWQQANYSEQ YCKTNFLHFR HLKRARDVKS QISMIFKKIG LKLISCHSDP DLIRKTFVSG FFMNAAKRDS QV GYKTING GTEVGIHPSS SLYGKEYEYV MYHSIVLTSR EYMSQVTSIE PQWLLEVAPH FYKAGDAESQ SRKKAKIIPL HNK FAKDQN SWRLSSIRQS RERALGIKR

Macromolecule #20: Pre-mRNA-splicing factor SYF2

• Macromolecule: Name: Pre-mRNA-splicing factor SYF2 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 24.850719 KDa

Sequence

String:

MDFYKLDEKL KELKRKRVDV SIKSRKLADR EIQEVSANRK PRVYSMEDVN DADESVGDTE SPEKEKAFHY TVQEYDAWER RHPQGKTGQ SQRGGISYDQ LAKLSYEKTL RNLATQTQNS SKQDSSADEE DNKNVPKKGR IGKVQKDTKT GKITIADDDK L VNKLAVSL QSESKKRYEA RKRQMQNAKT LYGVESFIND KNKQFNEKLS RESKGSE

Macromolecule #21: Pre-mRNA-splicing factor CEF1

• Macromolecule: Name: Pre-mRNA-splicing factor CEF1 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 67.837773 KDa

Sequence

String:

MPPVPIYVKG GVWTNVEDQI LKAAVQKYGT HQWSKVASLL QKKTARQSEL RWNEYLNPKL NFTEFSKEED AQLLDLAREL PNQWRTIAD MMARPAQVCV ERYNRLLESE DSGGAALSTG VTDLKAGDIN PNAETQMARP DNGDLEDEEK EMLAEARARL L NTQGKKAT RKIRERMLEE SKRIAELQKR RELKQAGINV AIKKPKKKYG TDIDYNEDIV YEQAPMPGIY DTSTEDRQIK KK FEQFERK VNRKGLDGNK DKPSKKNKDK KRKHDENEHV EKAALGESTT LTDEYKKPKL ILSAPGTKQG KVTYKKKLES KRQ KLIEAQ ATGTVLTPKE LLPHDSGQED NERSNIKSGK QLKSRIRKFL VQMFASLPSP KNDFEIVLSE DEKEEDAEIA EYEK EFENE RAMNEEDNFI EPPSQNDAPR VSLVAVPLAY STLPIPEFKN NPQSAIDNKY NLLVANAINK EPHMVPEDTV DFLKE VESR MQHITQGRTS MKIQFKTAMP PTEVLLESIQ SKVESIEQLQ RKLQHVQPLE QQNNEMCSTL CHHSLPALIE GQRKYY ADY YAYRQEIRSL EGRRKRLQAM LNSSSSI

Macromolecule #22: Pre-mRNA-splicing factor CLF1

• Macromolecule: Name: Pre-mRNA-splicing factor CLF1 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 82.555859 KDa

Sequence

String:

MDTLEPTAVD THVSAEQILR DVYKKGQKAR GSTNIDILDL EELREYQRRK RTEYEGYLKR NRLDMGQWIR YAQFEIEQHD MRRARSIFE RALLVDSSFI PLWIRYIDAE LKVKCINHAR NLMNRAISTL PRVDKLWYKY LIVEESLNNV EIVRSLYTKW C SLEPGVNA WNSFVDFEIR QKNWNGVREI YSKYVMAHPQ MQTWLKWVRF ENRHGNTEFT RSVYSLAIDT VANLQNLQIW SD MEVAKLV NSFAHWEAAQ QEYERSSALY QIAIEKWPSN QLLKAGLLDF EKQFGDINSI EETISYKRKM EYETILSNNA YDY DTWWLY LDLISESFPK QIMQTFEKAI VDSRPKELSK NVQWKRYIYL WMRYICYVEL ELENSLLEEE LFQRLIDDII PHKH FTFSK IWLMYAKFLI RHDDVPKARK ILGKAIGLCP KAKTFKGYIE LEVKLKEFDR VRKIYEKFIE FQPSDLQIWS QYGEL EENL GDWDRVRGIY TIALDENSDF LTKEAKIVLL QKYITFETES QEFEKARKLY RRYLELNQYS PQSWIEFAMY QTSTPT EQQ LLDLAKLQSE NVDEDIEFEI TDENKLEARK VFEEAIVFFK EKDDKQGRLS ILEALKDYEE TYGTELDQET VKKRFPK VI KKVRLQNGVE EEFVDYIFPD DIDDDKPKPS KFLELAKKWK QEQAL

Macromolecule #23: Pre-mRNA-splicing factor SYF1

• Macromolecule: Name: Pre-mRNA-splicing factor SYF1 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 100.344016 KDa

Sequence

String:

MSAYIAMKGV ITNVDENIRN DEDVAFEYEI QKTPQNILTW KRYIEYWKEE GRTDKQIRWL YERFCSQFVT DTSIWEDYIR WESTKEVVE TSRIFWLFQR CLKSCVRDCD RICLSYLELA IEQYDLAMIR HALASSLMKM EREMHRKVWD PVIKFVEEKV L PLTQLDST QEDEEESTDE AELINVLLVK GFTKGGFISE EISENGSRGD IWSSHILERY LKVAPQQKRN ESLATLALTR DN ITIKSVY EKYLPQDENS GKYLPSSELP FELNFNYLAS LEKLGLDNQY EEFMRQMNGI YPDKWLFLIL SLAKYYISRG RLD SCGDLL KKSLQQTLRY SDFDRIYNFY LLFEQECSQF ILGKLKENDS KFFNQKDWTE KLQAHMATFE SLINLYDIYL NDVA LRQDS NLVETWMKRV SLQKSAAEKC NVYSEAILKI DPRKVGTPGS FGRLWCSYGD LYWRSNAIST ARELWTQSLK VPYPY IEDL EEIYLNWADR ELDKEGVERA FSILEDALHV PTNPEILLEK YKNGHRKIPA QTVLFNSLRI WSKYIDYLEA YCPKDA NSS DKIFNKTKMA YNTVIDLRLI TPAMAENFAL FLQNHYEVME SFQVYEKTIP LFPPEIQYEL WIEYLEVATS HQLSSLS PE HIRFLFEKAL KNLCSNGIDC KTIFIAYSVF EERISGLISK SIEILRRGAV IGTVSVSTHL ESRLQLWRMC ISKAESTL G PSVTRELYQE CIQILPNSKA VEFVIKFSDF ESSIGETIRA REILAYGAKL LPPSRNTELW DSFEIFELKH GDKETYKDM LKMKKVLESN MLIDSASVSH EEGNINFVAA ATSHAPNSHT LTQSTSSYSI NPDEIELDI

Macromolecule #24: Unknown protein fragment

• Macromolecule: Name: Unknown protein fragment / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 18.65593 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

Macromolecule #25: Unknown protein fragment

• Macromolecule: Name: Unknown protein fragment / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 1.379692 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #26: Small nuclear ribonucleoprotein Sm D2

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D2 / type: protein_or_peptide / ID: 26 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 12.876066 KDa

Sequence

String:

MSSQIIDRPK HELSRAELEE LEEFEFKHGP MSLINDAMVT RTPVIISLRN NHKIIARVKA FDRHCNMVLE NVKELWTEKK GKNVINRER FISKLFLRGD SVIVVLKTPV E

Macromolecule #27: Small nuclear ribonucleoprotein F

• Macromolecule: Name: Small nuclear ribonucleoprotein F / type: protein_or_peptide / ID: 27 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 9.669945 KDa

Sequence

String:

MSESSDISAM QPVNPKPFLK GLVNHRVGVK LKFNSTEYRG TLVSTDNYFN LQLNEAEEFV AGVSHGTLGE IFIRCNNVLY IRELPN

Macromolecule #28: Small nuclear ribonucleoprotein E

• Macromolecule: Name: Small nuclear ribonucleoprotein E / type: protein_or_peptide / ID: 28 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 10.385098 KDa

Sequence

String:

MSNKVKTKAM VPPINCIFNF LQQQTPVTIW LFEQIGIRIK GKIVGFDEFM NVVIDEAVEI PVNSADGKED VEKGTPLGKI LLKGDNITL ITSAD

Macromolecule #29: Small nuclear ribonucleoprotein G

• Macromolecule: Name: Small nuclear ribonucleoprotein G / type: protein_or_peptide / ID: 29 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 8.490809 KDa

Sequence

String:

MVSTPELKKY MDKKILLNIN GSRKVAGILR GYDIFLNVVL DDAMEINGED PANNHQLGLQ TVIRGNSIIS LEALDAI

Macromolecule #30: Small nuclear ribonucleoprotein-associated protein B

• Macromolecule: Name: Small nuclear ribonucleoprotein-associated protein B / type: protein_or_peptide / ID: 30 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 22.42699 KDa

Sequence

String:

MSKIQVAHSS RLANLIDYKL RVLTQDGRVY IGQLMAFDKH MNLVLNECIE ERVPKTQLDK LRPRKDSKDG TTLNIKVEKR VLGLTILRG EQILSTVVED KPLLSKKERL VRDKKEKKQA QKQTKLRKEK EKKPGKIAKP NTANAKHTSS NSREIAQPSS S RYNGGNDN IGANRSRFNN EAPPQTRKFQ PPPGFKRK

Macromolecule #31: Small nuclear ribonucleoprotein Sm D3

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 31 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 11.240139 KDa

Sequence

String:

MTMNGIPVKL LNEAQGHIVS LELTTGATYR GKLVESEDSM NVQLRDVIAT EPQGAVTHMD QIFVRGSQIK FIVVPDLLKN APLFKKNSS RPMPPIRGPK RR

Macromolecule #32: Small nuclear ribonucleoprotein Sm D1

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D1 / type: protein_or_peptide / ID: 32 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 16.296798 KDa

Sequence

String:

MKLVNFLKKL RNEQVTIELK NGTTVWGTLQ SVSPQMNAIL TDVKLTLPQP RLNKLNSNGI AMASLYLTGG QQPTASDNIA SLQYINIRG NTIRQIILPD SLNLDSLLVD QKQLNSLRRS GQIANDPSKK RRRDFGAPAN KRPRRGL

Macromolecule #33: Lea1

• Macromolecule: Name: Lea1 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 12.850944 KDa

Sequence

String:

MVEPARKKQR IDRDTHHTVA EPVTEAKNTL YVSQLNEKIN MQRLRVNLFL LFATFGEVLK VSMNFKKQRG QAFITMRTID QASLAQISL NGERFFGKPL KVEFSKSETK TL

Macromolecule #34: Msl1

• Macromolecule: Name: Msl1 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 27.232252 KDa

Sequence

String:

MKFTPSIVID APQYYVDHFN GKYNVDKCVI LRDLQLETDS ESMPSSLKHL TKPTHILDLT NNDLIMIPDL SRRDDIHTLL LGRNNIVEV DGRLLPMNVQ NLTLSNNSIR RFEDLQRLRR APRTLKNLTL IGNQVCHLAN YREHVLRLVP HLETLDFQNV T AEERKSAM SFPRQADGDT LGPVNTAIRD NGSRDKTMEI MNLVVSKMTV ERRNELKKQL AEATSLEEIA RLEKLLSGGV

Macromolecule #35: Pre-mRNA-processing factor Prp19

• Macromolecule: Name: Pre-mRNA-processing factor Prp19 / type: protein_or_peptide / ID: 35 / Number of copies: 4 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 56.629777 KDa

Sequence

String:

MLCAISGKVP RRPVLSPKSR TIFEKSLLEQ YVKDTGNDPI TNEPLSIEEI VEIVPSAQQA SLTESTNSAT LKANYSIPNL LTSLQNEWD AIMLENFKLR STLDSLTKKL STVMYERDAA KLVAAQLLME KNEDSKDLPK SSQQAVAITR EEFLQGLLQS S RDFVARGK LKAPKWPILK NLELLQAQNY SRNIKTFPYK ELNKSMYYDK WVCMCRCEDG ALHFTQLKDS KTITTITTPN PR TGGEHPA IISRGPCNRL LLLYPGNQIT ILDSKTNKVL REIEVDSANE IIYMYGHNEV NTEYFIWADN RGTIGFQSYE DDS QYIVHS AKSDVEYSSG VLHKDSLLLA LYSPDGILDV YNLSSPDQAS SRFPVDEEAK IKEVKFADNG YWMVVECDQT VVCF DLRKD VGTLAYPTYT IPEFKTGTVT YDIDDSGKNM IAYSNESNSL TIYKFDKKTK NWTKDEESAL CLQSDTADFT DMDVV CGDG GIAAILKTND SFNIVALTP

Macromolecule #36: Pre-mRNA-splicing factor SNT309

• Macromolecule: Name: Pre-mRNA-splicing factor SNT309 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 20.741455 KDa

Sequence

String:

MDGLSFVDKG KIPDGYKNEI DQLVKKEFAN IKREPVHPEI RGILAKRKGA DNSVSTLTNA LYTEYLKQRN NKKRRTPDFN DDDDTLFLE EYRRKYPRID TSRYIPNESS EVSLLGIVDS YLKHQEIVLD TLLPQTVSNQ WRINNDYIRQ TCTIVEEMNI Q QRKQINDL EIYRKRL

Macromolecule #37: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 37 / Number of copies: 5 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #38: INOSITOL HEXAKISPHOSPHATE

• Macromolecule: Name: INOSITOL HEXAKISPHOSPHATE / type: ligand / ID: 38 / Number of copies: 1 / Formula: IHP
• Molecular weight: Theoretical: 660.035 Da

Chemical component information

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE / Phytic acid

Macromolecule #39: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 39 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

Macromolecule #40: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 40 / Number of copies: 7 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.9
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
• Image recording: Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 2.0 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Software - Name: CTFFIND (ver. 4)
• Initial angle assignment: Type: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 2.1)
• Final 3D classification: Software - Name: RELION (ver. 2.1)
• Final angle assignment: Type: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 2.1)
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2.1) / Number images used: 212219

Atomic model buiding 1

• Details: The author states the following for the pre-mRNA model building: For regions in the intron or exon where no clear sequence preferences were observed (Spingola et al., PMID 10024174), U, A, U, and A-U are used to represent a generic nucleotide, purine, pyrimidine, and standard Watson-Crick basepair, respectively, based on the EM density. Nucleotides -7 to +7 in the exon are modeled mostly as the preferred nucleotides in each position (Spingola et al. PMID 10024174) unless the density suggests otherwise. Some nucleotides in the intron seem to form basepairs with defined U2 or U6 snRNA sequence based on EM densities, and are modeled as complementary nucleotides to the corresponding U2 or U6 sequence unless the density suggests otherwise.

Output model

PDB-6bk8:
S. cerevisiae spliceosomal post-catalytic P complex