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- EMDB-38396: The Cryo-EM structure of MPXV E5 C-terminal in complex with DNA -
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Open data
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Basic information
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Title | The Cryo-EM structure of MPXV E5 C-terminal in complex with DNA | |||||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Zhang W / Liu Y / Gao H / Gan J | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural and functional insights into the helicase protein E5 of Mpox virus. Authors: Gan J / Zhang W | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 79 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 14.4 KB 14.4 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 9.2 KB | Display | ![]() |
Images | ![]() | 26.3 KB | ||
Filedesc metadata | ![]() | 5.7 KB | ||
Others | ![]() ![]() | 77.6 MB 77.6 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8xj8MC ![]() 8xifC ![]() 8xigC ![]() 8xj6C ![]() 8xj7C M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.081 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_38396_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_38396_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Monkeypox virus
Entire | Name: ![]() ![]() |
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Components |
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-Supramolecule #1: Monkeypox virus
Supramolecule | Name: Monkeypox virus / type: virus / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 / NCBI-ID: 10244 / Sci species name: Monkeypox virus / Virus type: VIRION / Virus isolate: SPECIES / Virus enveloped: Yes / Virus empty: Yes |
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-Macromolecule #1: DNA (70-MER)
Macromolecule | Name: DNA (70-MER) / type: dna / ID: 1 / Number of copies: 1 / Classification: DNA |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 21.440668 KDa |
Sequence | String: (DA)(DA)(DC)(DG)(DA)(DG)(DT)(DC)(DA)(DA) (DG)(DC)(DG)(DC)(DA)(DT)(DC)(DC)(DC)(DG) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT) (DT)(DT)(DT)(DT)(DT)(DT) ...String: (DA)(DA)(DC)(DG)(DA)(DG)(DT)(DC)(DA)(DA) (DG)(DC)(DG)(DC)(DA)(DT)(DC)(DC)(DC)(DG) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DC)(DG)(DG)(DG)(DA)(DT)(DG)(DC) (DG)(DC) (DT)(DT)(DG)(DA)(DC)(DT)(DC) (DG)(DT)(DT) |
-Macromolecule #2: Monkeypox virus E5
Macromolecule | Name: Monkeypox virus E5 / type: protein_or_peptide / ID: 2 / Number of copies: 6 / Enantiomer: LEVO EC number: ![]() |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 53.282012 KDa |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: GNKLFNIAQR ILDTNSVLLT ERGDHIVWIN NSWKFNSEEP LITKLILSIR HQLPKEYSSE LLCPRKRKTV EANIRDMLVD SVETDTYPD KLPFKNGVLD LVDGMFYSGD DAKKYTCTVS TGFKFDDTKF VEDSPEMEEL MNIINDIQPL TDENKKNREL Y EKTLSSCL ...String: GNKLFNIAQR ILDTNSVLLT ERGDHIVWIN NSWKFNSEEP LITKLILSIR HQLPKEYSSE LLCPRKRKTV EANIRDMLVD SVETDTYPD KLPFKNGVLD LVDGMFYSGD DAKKYTCTVS TGFKFDDTKF VEDSPEMEEL MNIINDIQPL TDENKKNREL Y EKTLSSCL CGATKGCLTF FFGETATGKS TTKRLLKSAI GDLFVETGQT ILTDVLDKGP NPFIANMHLK RSVFCSELPD FA CSGSKKI RSDNIKKLTE PCVIGRPCFS NKINNRNHAT IIIDTNYKPV FDRIDNALMR RIAVVRFRTH FSQPSGREAA ENN DAYDKV KLLDEGLDGK IQNNRYRFAF LYLLVKWYKK YHIPIMKLYP TPEEIPDFAF YLKIGTLLVS SSVKHIPLMT DLSK KGYIL YDNVVTLPLT TFQQKISKYF NSRLFGHDIE SFINRHKKFA NVSDEYLQYI FIEDISSP UniProtKB: Uncoating factor OPG117 |
-Macromolecule #3: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
Macromolecule | Name: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / type: ligand / ID: 3 / Number of copies: 3 / Formula: ANP |
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Molecular weight | Theoretical: 506.196 Da |
Chemical component information | ![]() ChemComp-ANP: |
-Macromolecule #4: MAGNESIUM ION
Macromolecule | Name: MAGNESIUM ION / type: ligand / ID: 4 / Number of copies: 3 / Formula: MG |
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Molecular weight | Theoretical: 24.305 Da |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |