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Open data
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Basic information
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Title | Cryo-EM structure of apo state mTRPV4 | |||||||||
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![]() | mTRPV4 / ![]() ![]() | |||||||||
Function / homology | ![]() blood vessel endothelial cell delamination / osmosensor activity / vasopressin secretion / stretch-activated, monoatomic cation-selective, calcium channel activity / positive regulation of striated muscle contraction / calcium ion import into cytosol / positive regulation of macrophage inflammatory protein 1 alpha production / negative regulation of brown fat cell differentiation / positive regulation of microtubule depolymerization / hyperosmotic salinity response ...blood vessel endothelial cell delamination / osmosensor activity / vasopressin secretion / stretch-activated, monoatomic cation-selective, calcium channel activity / positive regulation of striated muscle contraction / calcium ion import into cytosol / positive regulation of macrophage inflammatory protein 1 alpha production / negative regulation of brown fat cell differentiation / positive regulation of microtubule depolymerization / hyperosmotic salinity response / hypotonic response / cortical microtubule organization / positive regulation of chemokine (C-X-C motif) ligand 1 production / positive regulation of chemokine (C-C motif) ligand 5 production / cartilage development involved in endochondral bone morphogenesis / cellular hypotonic response / regulation of response to osmotic stress / cellular hypotonic salinity response / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Zhen WX / Yang F | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis of ligand activation and inhibition in a mammalian TRPV4 ion channel. Authors: Wenxuan Zhen / Zhijun Zhao / Shenghai Chang / Xiaoying Chen / Yangzhuoqun Wan / Fan Yang / ![]() | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 51.4 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 13.3 KB 13.3 KB | Display Display | ![]() |
Images | ![]() | 37.2 KB | ||
Filedesc metadata | ![]() | 5.3 KB | ||
Others | ![]() ![]() | 95.1 MB 95.1 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8j1dMC ![]() 8j1bC ![]() 8j1fC ![]() 8j1hC ![]() 8jkmC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 0.93 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_35919_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_35919_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : apo state of mTRPV4
Entire | Name: apo state of mTRPV4 |
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Components |
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-Supramolecule #1: apo state of mTRPV4
Supramolecule | Name: apo state of mTRPV4 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() ![]() ![]() |
-Macromolecule #1: Transient receptor potential cation channel subfamily V member 4
Macromolecule | Name: Transient receptor potential cation channel subfamily V member 4 type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() ![]() |
Molecular weight | Theoretical: 75.666594 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: APAPQPPPIL KVFNRPILFD IVSRGSTADL DGLLSFLLTH KKRLTDEEFR EPSTGKTCLP KALLNLSNGR NDTIPVLLDI AERTGNMRE FINSPFRDIY YRGQTSLHIA IERRCKHYVE LLVAQGADVH AQARGRFFQP KDEGGYFYFG ELPLSLAACT N QPHIVNYL ...String: APAPQPPPIL KVFNRPILFD IVSRGSTADL DGLLSFLLTH KKRLTDEEFR EPSTGKTCLP KALLNLSNGR NDTIPVLLDI AERTGNMRE FINSPFRDIY YRGQTSLHIA IERRCKHYVE LLVAQGADVH AQARGRFFQP KDEGGYFYFG ELPLSLAACT N QPHIVNYL TENPHKKADM RRQDSRGNTV LHALVAIADN TRENTKFVTK MYDLLLLKCS RLFPDSNLET VLNNDGLSPL MM AAKTGKI GVFQHIIRRE VTDEDTRHLS RKFKDWAYGP VYSSLYDLSS LDTCGEEVSV LEILVYNSKI ENRHEMLAVE PIN ELLRDK WRKFGAVSFY INVVSYLCAM VIFTLTAYYQ PLEGTPPYPY RTTVDYLRLA GEVITLFTGV LFFFTSIKDL FTKK CPGVN SLFVDGSFQL LYFIYSVLVV VSAALYLAGI EAYLAVMVFA LVLGWMNALY FTRGLKLTGT YSIMIQKILF KDLFR FLLV YLLFMIGYAS ALVTLLNPCT NMKVCDEDQS NCTVPTYPAC RDSETFSAFL LDLFKLTIGM GDLEMLSSAK YPVVFI LLL VTYIILTFVL LLNMLIALMG ETVGQVSKES KHIWKLQWAT TILDIERSFP VFLRKAFRSG EMVTVGKSSD GTPDRRW CF RVDEVNWSHW NQNLGIINED PGK UniProtKB: Transient receptor potential cation channel subfamily V member 4 |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Concentration | 5 mg/mL |
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Buffer | pH: 8 |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 52.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: OTHER |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.59 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 201371 |