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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | ecCTPS filament bound with CTP, NADH, DON | |||||||||
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Function / homology | ![]() CTP synthase (glutamine hydrolysing) / ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Guo CJ / Liu JL | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Filamentation and inhibition of prokaryotic CTP synthase Authors: Guo CJ / Liu JL | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 44.4 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 14 KB 14 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 8.9 KB | Display | ![]() |
Images | ![]() | 51.3 KB | ||
Masks | ![]() | 59.6 MB | ![]() | |
Filedesc metadata | ![]() | 5.5 KB | ||
Others | ![]() ![]() | 44.7 MB 44.7 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8i9oMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 1.06 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Density Histograms |
-Half map: #2
File | emd_35278_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_35278_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : ecCTPS filament with CTP,NADH,DON
Entire | Name: ecCTPS filament with CTP,NADH,DON |
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Components |
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-Supramolecule #1: ecCTPS filament with CTP,NADH,DON
Supramolecule | Name: ecCTPS filament with CTP,NADH,DON / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() ![]() |
-Macromolecule #1: CTP synthase
Macromolecule | Name: CTP synthase / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() ![]() |
Molecular weight | Theoretical: 60.317801 KDa |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: MTTNYIFVTG GVVSSLGKGI AAASLAAILE ARGLNVTIMK LDPYINVDPG TMSPIQHGEV FVTEDGAETD LDLGHYERFI RTKMSRRNN FTTGRIYSDV LRKERRGDYL GATVQVIPHI TNAIKERVLE GGEGHDVVLV EIGGTVGDIE SLPFLEAIRQ M AVEIGREH ...String: MTTNYIFVTG GVVSSLGKGI AAASLAAILE ARGLNVTIMK LDPYINVDPG TMSPIQHGEV FVTEDGAETD LDLGHYERFI RTKMSRRNN FTTGRIYSDV LRKERRGDYL GATVQVIPHI TNAIKERVLE GGEGHDVVLV EIGGTVGDIE SLPFLEAIRQ M AVEIGREH TLFMHLTLVP YMAASGEVKT KPTQHSVKEL LSIGIQPDIL ICRSDRAVPA NERAKIALFC NVPEKAVISL KD VDSIYKI PGLLKSQGLD DYICKRFSLN CPEANLSEWE QVIFEEANPV SEVTIGMVGK YIELPDAYKS VIEALKHGGL KNR VSVNIK LIDSQDVETR GVEILKGLDA ILVPGGFGYR GVEGMITTAR FARENNIPYL GICLGMQVAL IDYARHVANM ENAN STEFV PDCKYPVVAL ITEWRDENGN VEVRSEKSDL GGTMRLGAQQ CQLVDDSLVR QLYNAPTIVE RHRHRYEVNN MLLKQ IEDA GLRVAGRSGD DQLVEIIEVP NHPWFVACQF HPEFTSTPRD GHPLFAGFVK AASEFQKRQA UniProtKB: ![]() |
-Macromolecule #2: ADENINE
Macromolecule | Name: ADENINE / type: ligand / ID: 2 / Number of copies: 4 / Formula: ADE |
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Molecular weight | Theoretical: 135.127 Da |
Chemical component information | ![]() ChemComp-ADE: |
-Macromolecule #3: 5-OXO-L-NORLEUCINE
Macromolecule | Name: 5-OXO-L-NORLEUCINE / type: ligand / ID: 3 / Number of copies: 4 / Formula: ONL |
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Molecular weight | Theoretical: 145.156 Da |
Chemical component information | ![]() ChemComp-ONL: |
-Macromolecule #4: CYTIDINE-5'-TRIPHOSPHATE
Macromolecule | Name: CYTIDINE-5'-TRIPHOSPHATE / type: ligand / ID: 4 / Number of copies: 4 / Formula: CTP |
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Molecular weight | Theoretical: 483.156 Da |
Chemical component information | ![]() ChemComp-CTP: |
-Macromolecule #5: MAGNESIUM ION
Macromolecule | Name: MAGNESIUM ION / type: ligand / ID: 5 / Number of copies: 4 / Formula: MG |
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Molecular weight | Theoretical: 24.305 Da |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | filament |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |