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- EMDB-34544: Structure of the dimeric Xenopus tropical acid-sensitive outwardl... -
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Open data
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Basic information
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Title | Structure of the dimeric Xenopus tropical acid-sensitive outwardly rectifying channel ASOR trimer bound with tRNA (closed state) | |||||||||
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![]() | Chloride Channel tRNA Complex / ![]() | |||||||||
Function / homology | pH-gated chloride channel activity / TMEM206 protein / TMEM206 protein family / chloride transport / ![]() ![]() ![]() ![]() | |||||||||
Biological species | ![]() ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Chi P / Wang X / Li J / Li K / Zhang Y / Geng J / Wu J / Deng D | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure of the dimeric Xenopus tropical acid-sensitive outwardly rectifying channel ASOR trimer bound with tRNA (closed state) Authors: Chi P / Wang X / Li J / Li K / Zhang Y / Geng J / Wu J / Deng D | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 230.1 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 14.1 KB 14.1 KB | Display Display | ![]() |
Images | ![]() | 78.7 KB | ||
Filedesc metadata | ![]() | 5.7 KB | ||
Others | ![]() ![]() | 226.3 MB 226.3 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8h8eMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
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Voxel size | X=Y=Z: 0.83 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_34544_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_34544_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Complex of Xenopus tropical acid-sensitive outwardly rectifying c...
Entire | Name: Complex of Xenopus tropical acid-sensitive outwardly rectifying channel ASOR trimer and tRNA in digitonin |
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Components |
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-Supramolecule #1: Complex of Xenopus tropical acid-sensitive outwardly rectifying c...
Supramolecule | Name: Complex of Xenopus tropical acid-sensitive outwardly rectifying channel ASOR trimer and tRNA in digitonin type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() ![]() |
-Macromolecule #1: Proton-activated chloride channel
Macromolecule | Name: Proton-activated chloride channel / type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 41.031789 KDa |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: MEAIRKELSR SYQELNEEAE PVAIDPEEAE DEEKEQEEAA SAVAPDRDSD RSSPPVRFSR TCLKNFFSVL LILVYLLLMG VAVFLVYQT ITDFRDKLKH PVMSVSYKEV NMYDAPGIAL YPGKARLLSC EHHWYDHIPP LKDPGQPGEN TCVTQDISYI D PYTNKTMK ...String: MEAIRKELSR SYQELNEEAE PVAIDPEEAE DEEKEQEEAA SAVAPDRDSD RSSPPVRFSR TCLKNFFSVL LILVYLLLMG VAVFLVYQT ITDFRDKLKH PVMSVSYKEV NMYDAPGIAL YPGKARLLSC EHHWYDHIPP LKDPGQPGEN TCVTQDISYI D PYTNKTMK HALIVQGPRD VRRRELVFLQ FHLNETKQDF SAIDYLLFSS YEAFLKSHDQ VKFMQDCESS FSSWKFSGGF RT WVKMSLV KTKEEDGSQS VEFRQETSVV NFIDRRETPD KGDQLFFVVF EWKDPYIQEI QDIITANPWS MIALLCSVFL VLF KAADFA KLSVKWMIKV RRRHLKKRAR ELNHIS UniProtKB: Proton-activated chloride channel |
-Macromolecule #2: tRNA (75-MER)of Spodoptera frugiperda
Macromolecule | Name: tRNA (75-MER)of Spodoptera frugiperda / type: rna / ID: 2 / Number of copies: 1 |
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Source (natural) | Organism: ![]() ![]() ![]() |
Molecular weight | Theoretical: 24.108287 KDa |
Sequence | String: UCUUCGGUAG UAUAGUGGUC AGUAUCCCCG CCUGUCACGC GGGAGACCGG GGUUCGAUUC CCCGCCGGAG AGCCA |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 57.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: NONE |
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Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: ANGULAR RECONSTITUTION |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.81 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 182807 |