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基本情報
登録情報 | ![]() | |||||||||||||||
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タイトル | Cryo-EM structure of the PSI-LHCI-Lhcp supercomplex from Ostreococcus tauri | |||||||||||||||
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機能・相同性 | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() 類似検索 - 分子機能 | |||||||||||||||
生物種 | ![]() ![]() | |||||||||||||||
手法 | ![]() ![]() | |||||||||||||||
![]() | Shan J / Sheng X / Ishii A / Watanabe A / Song C / Murata K / Minagawa J / Liu Z | |||||||||||||||
資金援助 | ![]() ![]()
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![]() | ![]() タイトル: The photosystem I supercomplex from a primordial green alga harbors three light-harvesting complex trimers. 著者: Asako Ishii / Jianyu Shan / Xin Sheng / Eunchul Kim / Akimasa Watanabe / Makio Yokono / Chiyo Noda / Chihong Song / Kazuyoshi Murata / Zhenfeng Liu / Jun Minagawa / ![]() ![]() 要旨: As a ubiquitous picophytoplankton in the ocean and an early-branching green alga, is a model prasinophyte species for studying the functional evolution of the light-harvesting systems in ...As a ubiquitous picophytoplankton in the ocean and an early-branching green alga, is a model prasinophyte species for studying the functional evolution of the light-harvesting systems in photosynthesis. Here, we report the structure and function of the photosystem I (PSI) supercomplex in low light conditions, where it expands its photon-absorbing capacity by assembling with the light-harvesting complexes I (LHCI) and a prasinophyte-specific light-harvesting complex (Lhcp). The architecture of the supercomplex exhibits hybrid features of the plant-type and the green algal-type PSI supercomplexes, consisting of a PSI core, an Lhca1-Lhca4-Lhca2-Lhca3 belt attached on one side and an Lhca5-Lhca6 heterodimer associated on the other side between PsaG and PsaH. Interestingly, nine Lhcp subunits, including one Lhcp1 monomer with a phosphorylated amino-terminal threonine and eight Lhcp2 monomers, oligomerize into three trimers and associate with PSI on the third side between Lhca6 and PsaK. The Lhcp1 phosphorylation and the light-harvesting capacity of PSI were subjected to reversible photoacclimation, suggesting that the formation of PSI-LHCI-Lhcp supercomplex is likely due to a phosphorylation-dependent mechanism induced by changes in light intensity. Notably, this supercomplex did not exhibit far-red peaks in the 77 K fluorescence spectra, which is possibly due to the weak coupling of the chlorophyll 603-609 pair in Lhca1-4. | |||||||||||||||
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構造の表示
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-EMDBアーカイブ
マップデータ | ![]() | 202.7 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 48.3 KB 48.3 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 13.5 KB | 表示 | ![]() |
画像 | ![]() | 179 KB | ||
その他 | ![]() ![]() | 171.4 MB 171.4 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.04 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-ハーフマップ: #2
ファイル | emd_33737_half_map_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #1
ファイル | emd_33737_half_map_2.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
+全体 : photosystem I-light-harvesting complex I-prasinophyte-specific Lh...
+超分子 #1: photosystem I-light-harvesting complex I-prasinophyte-specific Lh...
+分子 #1: Lhca1
+分子 #2: Chlorophyll a-b binding protein, chloroplastic
+分子 #3: Chlorophyll a-b binding protein, chloroplastic
+分子 #4: Chlorophyll a-b binding protein, chloroplastic
+分子 #5: Chlorophyll a-b binding protein, chloroplastic
+分子 #6: Lhca6
+分子 #7: Photosystem I P700 chlorophyll a apoprotein A1
+分子 #8: Photosystem I P700 chlorophyll a apoprotein A2
+分子 #9: Photosystem I iron-sulfur center
+分子 #10: Photosystem I reaction center subunit II, chloroplastic
+分子 #11: Photosystem I reaction centre subunit IV
+分子 #12: Photosystem I reaction center subunit III
+分子 #13: PsaG
+分子 #14: Photosystem I PsaH, reaction centre subunit VI
+分子 #15: Photosystem I reaction center subunit VIII
+分子 #16: Photosystem I reaction center subunit IX
+分子 #17: Photosystem I PsaG/PsaK protein
+分子 #18: PSI subunit V
+分子 #19: Photosystem I reaction center subunit XII
+分子 #20: Photosystem I PsaN, reaction centre subunit N
+分子 #21: PsaO
+分子 #22: Chlorophyll a-b binding protein, chloroplastic
+分子 #23: Chlorophyll a-b binding protein, chloroplastic
+分子 #24: CHLOROPHYLL B
+分子 #25: CHLOROPHYLL A
+分子 #26: (3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-1-[(1~{S},4...
+分子 #27: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BE...
+分子 #28: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
+分子 #29: (1~{S})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E}...
+分子 #30: BETA-CAROTENE
+分子 #31: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
+分子 #32: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
+分子 #33: CHLOROPHYLL A ISOMER
+分子 #34: PHYLLOQUINONE
+分子 #35: IRON/SULFUR CLUSTER
+分子 #36: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
+分子 #37: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY...
+分子 #38: Chlorophyll c2
+分子 #39: water
-実験情報
-構造解析
手法 | ![]() |
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試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 6.5 |
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グリッド | モデル: Quantifoil R1.2/1.3 |
凍結 | 凍結剤: NITROGEN / チャンバー内湿度: 100 % / チャンバー内温度: 277 K |
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電子顕微鏡法
顕微鏡 | FEI TITAN |
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電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: OTHER / 撮影モード: BRIGHT FIELD![]() |
撮影 | フィルム・検出器のモデル: GATAN K2 BASE (4k x 4k) 平均電子線量: 60.0 e/Å2 |
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画像解析
-原子モデル構築 1
初期モデル | (Chain: PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model) |
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詳細 | Initial local fitting was done using Chimera and then Wincoot was usd for flexible fitting |
精密化 | 空間: REAL / プロトコル: RIGID BODY FIT / 温度因子: 110.967 / 当てはまり具合の基準: Correlation coefficient |
得られたモデル | ![]() PDB-7yca: |