+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-33396 | |||||||||
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タイトル | Hemichannel-focused structure of C-terminal truncated connexin43/Cx43/GJA1 gap junction intercellular channel in POPE nanodiscs (GCN-TM1i conformation) | |||||||||
マップデータ | unsharpened map | |||||||||
試料 |
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機能・相同性 | 機能・相同性情報 gap junction channel activity involved in cardiac conduction electrical coupling / negative regulation of gonadotropin secretion / positive regulation of striated muscle tissue development / 授乳 / positive regulation of morphogenesis of an epithelium / positive regulation of mesodermal cell differentiation / atrial ventricular junction remodeling / cell communication by chemical coupling / cell communication by electrical coupling / neuroblast migration ...gap junction channel activity involved in cardiac conduction electrical coupling / negative regulation of gonadotropin secretion / positive regulation of striated muscle tissue development / 授乳 / positive regulation of morphogenesis of an epithelium / positive regulation of mesodermal cell differentiation / atrial ventricular junction remodeling / cell communication by chemical coupling / cell communication by electrical coupling / neuroblast migration / gap junction hemi-channel activity / columnar/cuboidal epithelial cell maturation / negative regulation of trophoblast cell migration / regulation of bone remodeling / microtubule-based transport / monoatomic ion transmembrane transporter activity / SARS-CoV-2 targets PDZ proteins in cell-cell junction / glutathione transmembrane transporter activity / regulation of ventricular cardiac muscle cell membrane depolarization / gap junction channel activity involved in cell communication by electrical coupling / contractile muscle fiber / Oligomerization of connexins into connexons / Transport of connexins along the secretory pathway / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / gap junction assembly / cellular response to pH / atrial cardiac muscle cell action potential / Regulation of gap junction activity / skeletal muscle tissue regeneration / cardiac conduction system development / connexin complex / regulation of atrial cardiac muscle cell membrane depolarization / Formation of annular gap junctions / Golgi-associated vesicle membrane / Gap junction degradation / fascia adherens / bone remodeling / cell-cell contact zone / Gap junction assembly / gap junction channel activity / ギャップ結合 / export across plasma membrane / adult heart development / regulation of bone mineralization / glutamate secretion / xenobiotic transport / regulation of ventricular cardiac muscle cell membrane repolarization / 密着結合 / blood vessel morphogenesis / lens development in camera-type eye / cell communication by electrical coupling involved in cardiac conduction / 中間径フィラメント / embryonic digit morphogenesis / maintenance of blood-brain barrier / beta-tubulin binding / heart looping / positive regulation of stem cell proliferation / RHOJ GTPase cycle / RHOQ GTPase cycle / efflux transmembrane transporter activity / establishment of mitotic spindle orientation / 介在板 / alpha-tubulin binding / lateral plasma membrane / T cell proliferation / monoatomic ion transmembrane transport / positive regulation of vascular associated smooth muscle cell proliferation / tubulin binding / protein tyrosine kinase binding / neuron migration / 骨 / protein localization / negative regulation of cell growth / beta-catenin binding / osteoblast differentiation / cellular response to amyloid-beta / male gonad development / 細胞結合 / cell-cell signaling / positive regulation of cold-induced thermogenesis / heart development / scaffold protein binding / 精子形成 / positive regulation of canonical NF-kappaB signal transduction / in utero embryonic development / 脂質ラフト / apical plasma membrane / ゴルジ体 / negative regulation of gene expression / signaling receptor binding / intracellular membrane-bounded organelle / focal adhesion / endoplasmic reticulum membrane / positive regulation of gene expression / ゴルジ体 / シグナル伝達 / ミトコンドリア / 核質 / 細胞核 / 細胞膜 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.8 Å | |||||||||
データ登録者 | Lee HJ / Cha HJ / Jeong H / Lee SN / Lee CW / Woo JS | |||||||||
資金援助 | 韓国, 1件
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引用 | ジャーナル: Nat Commun / 年: 2023 タイトル: Conformational changes in the human Cx43/GJA1 gap junction channel visualized using cryo-EM. 著者: Hyuk-Joon Lee / Hyung Jin Cha / Hyeongseop Jeong / Seu-Na Lee / Chang-Won Lee / Minsoo Kim / Jejoong Yoo / Jae-Sung Woo / 要旨: Connexin family proteins assemble into hexameric hemichannels in the cell membrane. The hemichannels dock together between two adjacent membranes to form gap junction intercellular channels (GJIChs). ...Connexin family proteins assemble into hexameric hemichannels in the cell membrane. The hemichannels dock together between two adjacent membranes to form gap junction intercellular channels (GJIChs). We report the cryo-electron microscopy structures of Cx43 GJICh, revealing the dynamic equilibrium state of various channel conformations in detergents and lipid nanodiscs. We identify three different N-terminal helix conformations of Cx43-gate-covering (GCN), pore-lining (PLN), and flexible intermediate (FIN)-that are randomly distributed in purified GJICh particles. The conformational equilibrium shifts to GCN by cholesteryl hemisuccinates and to PLN by C-terminal truncations and at varying pH. While GJIChs that mainly comprise GCN protomers are occluded by lipids, those containing conformationally heterogeneous protomers show markedly different pore sizes. We observe an α-to-π-helix transition in the first transmembrane helix, which creates a side opening to the membrane in the FIN and PLN conformations. This study provides basic structural information to understand the mechanisms of action and regulation of Cx43 GJICh. | |||||||||
履歴 |
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-構造の表示
添付画像 |
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-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_33396.map.gz | 48.1 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-33396-v30.xml emd-33396.xml | 14.3 KB 14.3 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_33396_fsc.xml | 9.2 KB | 表示 | FSCデータファイル |
画像 | emd_33396.png | 8.7 KB | ||
その他 | emd_33396_half_map_1.map.gz emd_33396_half_map_2.map.gz | 48.4 MB 48.4 MB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-33396 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-33396 | HTTPS FTP |
-関連構造データ
関連構造データ | 7xqhMC 7f92C 7f93C 7f94C 7xq9C 7xqbC 7xqdC 7xqfC 7xqgC 7xqiC 7xqjC M: このマップから作成された原子モデル C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_33396.map.gz / 形式: CCP4 / 大きさ: 64 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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注釈 | unsharpened map | ||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 0.848 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-ハーフマップ: half map
ファイル | emd_33396_half_map_1.map | ||||||||||||
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注釈 | half map | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: half map
ファイル | emd_33396_half_map_2.map | ||||||||||||
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注釈 | half map | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-試料の構成要素
-全体 : Dodecameric complex of C-terminal deletion mutant of human Cx43/G...
全体 | 名称: Dodecameric complex of C-terminal deletion mutant of human Cx43/GJA1 (Cx43-M257) in lipid nanodiscs (POPE) |
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要素 |
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-超分子 #1: Dodecameric complex of C-terminal deletion mutant of human Cx43/G...
超分子 | 名称: Dodecameric complex of C-terminal deletion mutant of human Cx43/GJA1 (Cx43-M257) in lipid nanodiscs (POPE) タイプ: complex / ID: 1 / キメラ: Yes / 親要素: 0 / 含まれる分子: all 詳細: Hemichannel of Human Cx43-M257 gap junction channel with gate-covering NTH and TM1-intermediate conformation |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
-分子 #1: C-terminal deletion mutant of gap junction alpha-1 protein (Cx43-M257)
分子 | 名称: C-terminal deletion mutant of gap junction alpha-1 protein (Cx43-M257) タイプ: protein_or_peptide / ID: 1 / コピー数: 6 / 光学異性体: LEVO |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 29.396547 KDa |
組換発現 | 生物種: Homo sapiens (ヒト) |
配列 | 文字列: MGDWSALGKL LDKVQAYSTA GGKVWLSVLF IFRILLLGTA VESAWGDEQS AFRCNTQQPG CENVCYDKSF PISHVRFWVL QIIFVSVPT LLYLAHVFYV MRKEEKLNKK EEELKVAQTD GVNVDMHLKQ IEIKKFKYGI EEHGKVKMRG GLLRTYIISI L FKSIFEVA ...文字列: MGDWSALGKL LDKVQAYSTA GGKVWLSVLF IFRILLLGTA VESAWGDEQS AFRCNTQQPG CENVCYDKSF PISHVRFWVL QIIFVSVPT LLYLAHVFYV MRKEEKLNKK EEELKVAQTD GVNVDMHLKQ IEIKKFKYGI EEHGKVKMRG GLLRTYIISI L FKSIFEVA FLLIQWYIYG FSLSAVYTCK RDPCPHQVDC FLSRPTEKTI FIIFMLVVSL VSLALNIIEL FYVFFKGVKD RV KGKSDPY HATSGALSPA |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 8 |
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凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELDBright-field microscopy / 最大 デフォーカス(公称値): 2.4 µm / 最小 デフォーカス(公称値): 0.9 µm |
撮影 | フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) 平均電子線量: 72.42 e/Å2 |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |