EMDB-26624: Composite cryo-EM density map of the 48-nm repeat of the human re...

Basic information

Entry

Database: EMDB / ID: EMD-26624

• Title: Composite cryo-EM density map of the 48-nm repeat of the human respiratory doublet microtubule
• Map data: phenix auto-sharpened map

Sample

• Complex: Doublet microtubule and associated proteins
  • Protein or peptide: x 35 types
• Ligand: x 3 types

Function / homology

Function:

outer acrosomal membrane / epithelial cilium movement involved in determination of left/right asymmetry / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium / regulation of calcineurin-NFAT signaling cascade / sperm axoneme assembly / inner dynein arm assembly / cilium-dependent cell motility / protein polyglutamylation / regulation of microtubule nucleation / positive regulation of feeding behavior / cerebrospinal fluid circulation / sperm principal piece / regulation of cilium beat frequency involved in ciliary motility / cilium movement involved in cell motility / 9+2 motile cilium / regulation of store-operated calcium entry / cilium movement / Transferases; Transferring phosphorus-containing groups / calcium ion sensor activity / acrosomal membrane / axoneme assembly / Post-chaperonin tubulin folding pathway / flagellated sperm motility / cilium organization / axonemal microtubule / left/right axis specification / Carboxyterminal post-translational modifications of tubulin / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / gamma-tubulin ring complex / organelle transport along microtubule / glial cell differentiation / forebrain morphogenesis / cytoskeleton-dependent intracellular transport / Sealing of the nuclear envelope (NE) by ESCRT-III / Intraflagellar transport / neuron projection arborization / manchette / positive regulation of cilium assembly / Gap junction assembly / cerebellar cortex morphogenesis / Formation of tubulin folding intermediates by CCT/TriC / dentate gyrus development / COPI-independent Golgi-to-ER retrograde traffic / pyramidal neuron differentiation / natural killer cell mediated cytotoxicity / Prefoldin mediated transfer of substrate to CCT/TriC / UTP biosynthetic process / Kinesins / CTP biosynthetic process / Assembly and cell surface presentation of NMDA receptors / motile cilium / positive regulation of cell motility / determination of left/right symmetry / GTP biosynthetic process / centrosome cycle / intermediate filament / motor behavior / COPI-dependent Golgi-to-ER retrograde traffic / microtubule organizing center / response to L-glutamate / ciliary base / smoothened signaling pathway / intercellular bridge / regulation of synapse organization / beta-tubulin binding / regulation of neuron projection development / AMP binding / regulation of cell division / startle response / spermatid development / Recycling pathway of L1 / locomotory exploration behavior / microtubule polymerization / cerebral cortex cell migration / axoneme / single fertilization / mitotic cytokinesis / alpha-tubulin binding / cilium assembly / sperm flagellum / cellular response to UV-C / RHO GTPases activate IQGAPs / cellular response to unfolded protein / MHC class I protein binding / Hedgehog 'off' state / response to tumor necrosis factor / cytoplasmic microtubule / microtubule-based process / COPI-mediated anterograde transport / Activation of AMPK downstream of NMDARs / response to mechanical stimulus / Mitotic Prometaphase / homeostasis of number of cells within a tissue / EML4 and NUDC in mitotic spindle formation / condensed chromosome / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome

Domain/homology:

Protein of unknown function DUF1143 / Protein of unknown function DUF4541 / Protein of unknown function (DUF1143) / Domain of unknown function (DUF4541) / FAM183 family / FAM183A and FAM183B related / Sperm-associated antigen 8 / Sperm acrosome-associated protein 9 / Cilia- and flagella-associated protein 95 / NDPK7, first NDPk domain / Cilia- and flagella-associated protein 107 / : / Sperm acrosome-associated protein 9 / Protein CFAP95 / Tektin / Cilia- and flagella-associated protein 276 / Tektin family / Protein of unknown function (DUF3695) / Cilia- and flagella-associated protein 77 / Cilia- and flagella-associated protein 77 / Uncharacterised protein FAM166/UPF0605 / Protein Flattop / Protein of unknown function (DUF2475) / Nucleoside diphosphate kinase 7 / Meiosis-specific nuclear structural protein 1 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / Calmodulin-binding / Enkurin domain profile. / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / Parkin co-regulated protein / Parkin co-regulated protein / Nucleoside diphosphate kinase (NDPK)-like domain profile. / Nucleoside diphosphate kinase / Nucleoside diphosphate kinase-like domain / Nucleoside diphosphate kinase / NDK / Nucleoside diphosphate kinase-like domain superfamily / Tubulin-beta mRNA autoregulation signal. / Alpha tubulin / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily

Component:

Cilia- and flagella-associated protein 90 / Ciliary microtubule inner protein 2C / Cilia- and flagella-associated protein 144 / Ciliary microtubule inner protein 2B / Piercer of microtubule wall 2 protein / Tubulin beta-4B chain / Cilia- and flagella- associated protein 210 / Piercer of microtubule wall 1 protein / EF-hand domain-containing family member C2 / EF-hand domain-containing protein 1 / Cilia- and flagella-associated protein 276 / Protein Flattop / Cilia- and flagella-associated protein 95 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 161 / Cilia- and flagella-associated protein 77 / Tubulin alpha-1A chain / Cilia- and flagella-associated protein 107 / Cilia- and flagella-associated protein 52 / EF-hand domain-containing family member B / Meiosis-specific nuclear structural protein 1 / Enkurin / Tektin-4 / Tektin-1 / Sperm acrosome-associated protein 9 / Cilia- and flagella-associated protein 53 / Parkin coregulated gene protein / Sperm-associated antigen 8 / Tektin-3 / RIB43A-like with coiled-coils protein 2 / Cilia- and flagella-associated protein 68 / Tektin-2 / Cilia- and flagella-associated protein 45 / Nucleoside diphosphate kinase homolog 7 / Cilia- and flagella-associated protein 20

• Biological species: Homo sapiens (human) / human (human)
• Method: single particle reconstruction / cryo EM / Resolution: 3.6 Å
• Authors: Gui M / Croft JT / Zabeo D / Acharya V / Kollman JM / Burgoyne T / Hoog JL / Brown A

Funding support

United States, Sweden, 6 items

Organization	Grant number	Country
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	GM141109	United States
Swedish Research Council	2015-05427	Sweden
Swedish Research Council	2019-04004	Sweden
Smith Family Foundation		United States
Pew Charitable Trusts		United States
Charles A. King Trust	Postdoctoral Research Fellowship	United States

• Citation: Journal: Proc Natl Acad Sci U S A / Year: 2022; Title: SPACA9 is a lumenal protein of human ciliary singlet and doublet microtubules.; Authors: Miao Gui / Jacob T Croft / Davide Zabeo / Vajradhar Acharya / Justin M Kollman / Thomas Burgoyne / Johanna L Höög / Alan Brown / ; Abstract: The cilium-centrosome complex contains triplet, doublet, and singlet microtubules. The lumenal surfaces of each microtubule within this diverse array are decorated by microtubule inner proteins (MIPs). Here, we used single-particle cryo-electron microscopy methods to build atomic models of two types of human ciliary microtubule: the doublet microtubules of multiciliated respiratory cells and the distal singlet microtubules of monoflagellated human spermatozoa. We discover that SPACA9 is a polyspecific MIP capable of binding both microtubule types. SPACA9 forms intralumenal striations in the B tubule of respiratory doublet microtubules and noncontinuous spirals in sperm singlet microtubules. By acquiring new and reanalyzing previous cryo-electron tomography data, we show that SPACA9-like intralumenal striations are common features of different microtubule types in animal cilia. Our structures provide detailed references to help rationalize ciliopathy-causing mutations and position cryo-EM as a tool for the analysis of samples obtained directly from ciliopathy patients.

History

Deposition	Apr 11, 2022	-
Header (metadata) release	Oct 5, 2022	-
Map release	Oct 5, 2022	-
Update	Oct 19, 2022	-
Current status	Oct 19, 2022	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_26624.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: phenix auto-sharpened map
• Voxel size: X=Y=Z: 1.37 Å

Density

Contour Level	By AUTHOR: 1.5
Minimum - Maximum	-13.574953 - 24.73336
Average (Standard dev.)	-1.2295373e-12 (±1.0)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 512 512 512 Spacing 512 512 512
Cell	A=B=C: 701.44 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_26624_msk_1.map

Additional map: local masked map

• File: emd_26624_additional_1.map
• Annotation: local masked map

Additional map: stitched unsharpened map

• File: emd_26624_additional_10.map
• Annotation: stitched unsharpened map

Additional map: consensus refined map

• File: emd_26624_additional_11.map
• Annotation: consensus refined map

Additional map: local masked map

• File: emd_26624_additional_12.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_13.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_14.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_15.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_16.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_17.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_18.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_19.map
• Annotation: local masked map

Additional map: deepEMhancer sharpened map

• File: emd_26624_additional_2.map
• Annotation: deepEMhancer sharpened map

Additional map: local masked map

• File: emd_26624_additional_20.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_21.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_22.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_23.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_24.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_25.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_26.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_27.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_28.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_29.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_3.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_30.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_31.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_32.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_33.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_4.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_5.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_6.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_7.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_8.map
• Annotation: local masked map

Additional map: local masked map

• File: emd_26624_additional_9.map
• Annotation: local masked map

Half map: #2

• File: emd_26624_half_map_1.map

Half map: #1

• File: emd_26624_half_map_2.map

Sample components

Entire : Doublet microtubule and associated proteins

• Entire: Name: Doublet microtubule and associated proteins

Components

• Complex: Doublet microtubule and associated proteins
  • Protein or peptide: Protein CFAP95
  • Protein or peptide: EF-hand domain-containing family member B
  • Protein or peptide: Cilia- and flagella-associated protein 53
  • Protein or peptide: Nucleoside diphosphate kinase 7Nucleoside-diphosphate kinase
  • Protein or peptide: Protein CFAP107
  • Protein or peptide: Protein CFAP141
  • Protein or peptide: Tektin-1
  • Protein or peptide: Tubulin alpha-1A chain
  • Protein or peptide: Tubulin beta-4B chain
  • Protein or peptide: Meiosis-specific nuclear structural protein 1
  • Protein or peptide: Tektin-2
  • Protein or peptide: Tektin-3
  • Protein or peptide: Sperm-associated antigen 8
  • Protein or peptide: Tektin-4
  • Protein or peptide: Cilia- and flagella-associated protein 161
  • Protein or peptide: Sperm acrosome-associated protein 9
  • Protein or peptide: Uncharacterized protein C15orf65
  • Protein or peptide: Protein FAM166B
  • Protein or peptide: UPF0686 protein C11orf1
  • Protein or peptide: Isoform 2 of Cilia- and flagella-associated protein 77
  • Protein or peptide: Protein FAM183A
  • Protein or peptide: Uncharacterized protein C5orf49
  • Protein or peptide: Protein FAM166C
  • Protein or peptide: RIB43A-like with coiled-coils protein 2
  • Protein or peptide: EF-hand domain-containing protein 1
  • Protein or peptide: EF-hand domain-containing family member C2
  • Protein or peptide: Cilia- and flagella-associated protein 20
  • Protein or peptide: Parkin coregulated gene protein
  • Protein or peptide: Cilia- and flagella-associated protein 45
  • Protein or peptide: Cilia- and flagella-associated protein 52
  • Protein or peptide: Enkurin
  • Protein or peptide: Protein Flattop
  • Protein or peptide: Protein CFAP210
  • Protein or peptide: Protein CFAP276
  • Protein or peptide: UPF0691 protein C9orf116
• Ligand: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate
• Ligand: MAGNESIUM ION
• Ligand: GUANOSINE-5'-DIPHOSPHATE

Supramolecule #1: Doublet microtubule and associated proteins

• Supramolecule: Name: Doublet microtubule and associated proteins / type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: #1-#35
• Source (natural): Organism: Homo sapiens (human)

Macromolecule #1: Protein CFAP95

• Macromolecule: Name: Protein CFAP95 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 26.483875 KDa

Sequence

String:

MDSLDRSCQD WCDRKQHWLE IGPPDLVERK GSLTLRSHHK KYSKPVLVYS WHRDREAFPK GYDIEGPEKV KKLCNSTYRR LGTDESPIW TSETHEKLSQ MCLNTEWVEM KSKALLNEET VSSGIIERVT GLPATGFGAV FPRHPPDWSK MCALTTYSED Y VPPYDYQP HAYPCQDDYS IVHRKCRSQF TDLNGSKRFG INTWHDESGI YANSDVKQKL YPLTSGPIVP I

Macromolecule #2: EF-hand domain-containing family member B

• Macromolecule: Name: EF-hand domain-containing family member B / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 93.940008 KDa

Sequence

String:

MNMEIGHPHE GKDDLGDKRV IMGTKFPMEL GIRVGLGKED SRCGESPVVS NKCEGRMAPP ETKFPLSKGL EMGLERQNIS RTVMQRGSL GVDSVSASQG TKPSLLPGRM GLENESLLAG YTHERIIQPP LGRVCGSSQA AGSRRAPLAS GPEGVEELVG K PAFVMEPR QEMEKESTCV LMKPNTEIKL PVEVDIGLTQ AEGPDETKNT EPQMGLVIEP PQCQFAQQHE QRKEAGNIES GV EPPDRIR PIYSGKFFDR TPCWPSAGKV IPVGYRVATC LTEKLPRLIT PPEAKKYFNF RYPPAGVERV FYGRANDPQI APY LTHGIR SKISVLANTL INPQPITTFQ QKIKDKKESI YLSNRRAPLG KSHDQAPGLP KGMDTTNTTF GTAVIKEYSA KDVV NPPKS YEEVFKEGNE GHDLYVVSHN DYYAGEAKNR KYNPSSFHRC SVYGVPTPHF NDGRAMAKSL YWLHELQMKR GAKFV SKRA DDFKEKFQHK LGRVLDPIAE TMNVPPDCTF GACLRPEEYG VGDLIHNRLP DEYLRGKDRQ RALIAAVRHH LKKVNY QKF DTLLAAFRHY DKKGDGMIDK DELQEACDQA NLSLDDKLLD QLFDYCDVDN DGFINYLEFA NFLNWKDKML LKEYEER VI IKGRKPDCVN PTEANVEEPE QTLLIKPEDI VLKEAGSTEK TLRTLLRPSD KVSNYYKTTS SEINAIVGAI PSTCYPIC G VPTIRSDIPA PRIRRISDRT NYGEEGSAYS LLYPTIFARK GVFERDFFKT RSKEEIAEIL CNIGVKLSDE EFENVWNLA SKKHHRGEVC VENIRNVLDE LRHADRIKCK TLM

Macromolecule #3: Cilia- and flagella-associated protein 53

• Macromolecule: Name: Cilia- and flagella-associated protein 53 / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 61.960844 KDa

Sequence

String:

MYSQRFGTVQ REVKGPTPKV VIVRSKPPKG QGAEHHLERI RRSHQKHNAI LASIKSSERD RLKAEWDQHN DCKILDSLVR ARIKDAVQG FIINIEERRN KLRELLALEE NEYFTEMQLK KETIEEKKDR MREKTKLLKE KNEKERQDFV AEKLDQQFRE R CEELRVEL LSIHQKKVCE ERKAQIAFNE ELSRQKLVEE QMFSKLWEED RLAKEKREAQ EARRQKELME NTRLGLNAQI TS IKAQRQA TQLLKEEEAR LVESNNAQIK HENEQDMLKK QKAKQETRTI LQKALQERIE HIQQEYRDEQ DLNMKLVQRA LQD LQEEAD KKKQKREDMI REQKIYHKYL AQRREEEKAQ EKEFDRILEE DKAKKLAEKD KELRLEKEAR RQLVDEVMCT RKLQ VQEKL QREAKEQEER AMEQKHINES LKELNCEEKE NFARRQRLAQ EYRKQLQMQI AYQQQSQEAE KEEKRREFEA GVAAN KMCL DKVQEVLSTH QVLPQNIHPM RKACPSKLPP

Macromolecule #4: Nucleoside diphosphate kinase 7

• Macromolecule: Name: Nucleoside diphosphate kinase 7 / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO / EC number: nucleoside-diphosphate kinase
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 42.545707 KDa

Sequence

String:

MNHSERFVFI AEWYDPNASL LRRYELLFYP GDGSVEMHDV KNHRTFLKRT KYDNLHLEDL FIGNKVNVFS RQLVLIDYGD QYTARQLGS RKEKTLALIK PDAISKAGEI IEIINKAGFT ITKLKMMMLS RKEALDFHVD HQSRPFFNEL IQFITTGPII A MEILRDDA ICEWKRLLGP ANSGVARTDA SESIRALFGT DGIRNAAHGP DSFASAAREM ELFFPSSGGC GPANTAKFTN CT CCIVKPH AVSEGLLGKI LMAIRDAGFE ISAMQMFNMD RVNVEEFYEV YKGVVTEYHD MVTEMYSGPC VAMEIQQNNA TKT FREFCG PADPEIARHL RPGTLRAIFG KTKIQNAVHC TDLPEDGLLE VQYFFKILDN

Macromolecule #5: Protein CFAP107

• Macromolecule: Name: Protein CFAP107 / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 23.105348 KDa

Sequence

String:

MFLTAVNPQP LSTPSWQIET KYSTKVLTGN WMEERRKFTR DTDKTPQSIY RKEYIPFPDH RPDQISRWYG KRKVEGLPYK HLITHHQEP PHRYLISTYD DHYNRHGYNP GLPPLRTWNG QKLLWLPEKS DFPLLAPPTN YGLYEQLKQR QLTPKAGLKQ S TYTSSYPR PPLCAMSWRE HAVPVPPHRL HPFPHF

Macromolecule #6: Protein CFAP141

• Macromolecule: Name: Protein CFAP141 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 12.151156 KDa

Sequence

String:

MSVEKMTKVE ESFQKAMGLK KTVDRWRNSH THCLWQMALG QRRNPYATLR MQDTMVQELA LAKKQLLMVR QAALHQLFEK EHQQYQQEL NQMGKAFYVE RF

Macromolecule #7: Tektin-1

• Macromolecule: Name: Tektin-1 / type: protein_or_peptide / ID: 7 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 48.360113 KDa

Sequence

String:

MAKLLQPPPK FLPSEWHIAN KNQYHRADAQ RSRSERLVAE SQRLVDEIEK TTRKSQSDVN KKLEQRLEEV QFWKKELDDK LEQLVNVTD DLLIYKIRLE KALETLKEPL HITETCLAYR EKRIGIDLVH DTVEHELIKE AEIIQGIMAL LTRTLEEASE Q IRMNRSAK YNLEKDLKDK FVALTIDDIC FSLNNNSPNI RYSENAVRIE PNSVSLEDWL DFSSTNVEKA DKQRNNSLML KA LVDRILS QTANDLRKQC DVVDTAFKNG LKDTKDARDK LADHLAKVME EIASQEKNIT ALEKAILDQE GPAKVAHTRL ETR THRPNV ELCRDVAQYR LMKEVQEITH NVARLKETLA QAQAELKGLH RRQLALQEEI QVKENTIYID EVLCMQMRKS IPLR DGEDH GVWAGGLRPD AVC

Macromolecule #8: Tubulin alpha-1A chain

• Macromolecule: Name: Tubulin alpha-1A chain / type: protein_or_peptide / ID: 8 / Number of copies: 149 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 50.188441 KDa

Sequence

String:

MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK HVPRAVFVDL EPTVIDEVRT GTYRQLFHP EQLITGKEDA ANNYARGHYT IGKEIIDLVL DRIRKLADQC TGLQGFLVFH SFGGGTGSGF TSLLMERLSV D YGKKSKLE FSIYPAPQVS TAVVEPYNSI LTTHTTLEHS DCAFMVDNEA IYDICRRNLD IERPTYTNLN RLIGQIVSSI TA SLRFDGA LNVDLTEFQT NLVPYPRIHF PLATYAPVIS AEKAYHEQLS VAEITNACFE PANQMVKCDP RHGKYMACCL LYR GDVVPK DVNAAIATIK TKRTIQFVDW CPTGFKVGIN YQPPTVVPGG DLAKVQRAVC MLSNTTAIAE AWARLDHKFD LMYA KRAFV HWYVGEGMEE GEFSEAREDM AALEKDYEEV GVDSVEGEGE EEGEEY

Macromolecule #9: Tubulin beta-4B chain

• Macromolecule: Name: Tubulin beta-4B chain / type: protein_or_peptide / ID: 9 / Number of copies: 153 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 49.877824 KDa

Sequence

String:

MREIVHLQAG QCGNQIGAKF WEVISDEHGI DPTGTYHGDS DLQLERINVY YNEATGGKYV PRAVLVDLEP GTMDSVRSGP FGQIFRPDN FVFGQSGAGN NWAKGHYTEG AELVDSVLDV VRKEAESCDC LQGFQLTHSL GGGTGSGMGT LLISKIREEY P DRIMNTFS VVPSPKVSDT VVEPYNATLS VHQLVENTDE TYCIDNEALY DICFRTLKLT TPTYGDLNHL VSATMSGVTT CL RFPGQLN ADLRKLAVNM VPFPRLHFFM PGFAPLTSRG SQQYRALTVP ELTQQMFDAK NMMAACDPRH GRYLTVAAVF RGR MSMKEV DEQMLNVQNK NSSYFVEWIP NNVKTAVCDI PPRGLKMSAT FIGNSTAIQE LFKRISEQFT AMFRRKAFLH WYTG EGMDE MEFTEAESNM NDLVSEYQQY QDATAEEEGE FEEEAEEEVA

Macromolecule #10: Meiosis-specific nuclear structural protein 1

• Macromolecule: Name: Meiosis-specific nuclear structural protein 1 / type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 60.687531 KDa

Sequence

String:

MGSKRRNLSC SERHQKLVDE NYCKKLHVQA LKNVNSQIRN QMVQNENDNR VQRKQFLRLL QNEQFELDME EAIQKAEENK RLKELQLKQ EEKLAMELAK LKHESLKDEK MRQQVRENSI ELRELEKKLK AAYMNKERAA QIAEKDAIKY EQMKRDAEIA K TMMEEHKR IIKEENAAED KRNKAKAQYY LDLEKQLEEQ EKKKQEAYEQ LLKEKLMIDE IVRKIYEEDQ LEKQQKLEKM NA MRRYIEE FQKEQALWRK KKREEMEEEN RKIIEFANMQ QQREEDRMAK VQENEEKRLQ LQNALTQKLE EMLRQREDLE QVR QELYQE EQAEIYKSKL KEEAEKKLRK QKEMKQDFEE QMALKELVLQ AAKEEEENFR KTMLAKFAED DRIELMNAQK QRMK QLEHR RAVEKLIEER RQQFLADKQR ELEEWQLQQR RQGFINAIIE EERLKLLKEH ATNLLGYLPK GVFKKEDDID LLGEE FRKV YQQRSEICEE K

Macromolecule #11: Tektin-2

• Macromolecule: Name: Tektin-2 / type: protein_or_peptide / ID: 11 / Number of copies: 10 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 49.743176 KDa

Sequence

String:

MATLSVKPSR RFQLPDWHTN SYLLSTNAQL QRDASHQIRQ EARVLRNETN NQTIWDEHDN RTRLVERIDT VNRWKEMLDK CLTDLDAEI DALTQMKESA EQNLQAKNLP LDVAIECLTL RESRRDIDVV KDPVEDELHK EVEVIEATKK ALQQKVSQAF E QLCLLQEV QQQLNSDHRG KMETLEIDRG CLSLNLRSPN ISLKVDPTRV PDGSTTLQQW DDFSRFNKDR AEAEMKAATE LR EATALTI AETNNELEAQ RVATEFAFRK RLREMEKVYS ELKWQEKNTL EEIAELQEDI RHLEEDLRTK LLSLKLSHTR LEA RTYRPN VELCRDQAQY GLTDEVHQLE ATIAALKQKL AQAQDALDAL CKHLARLQAD IACKANSMLL DTKCMDTRRK LTVP AERFV PEVDTFTRTT NSTLSPLKSC QLELA

Macromolecule #12: Tektin-3

• Macromolecule: Name: Tektin-3 / type: protein_or_peptide / ID: 12 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 56.717902 KDa

Sequence

String:

MERVGCTLTT TYAHPRPTPT NFLPAISTMA SSYRDRFPHS NLTHSLSLPW RPSTYYKVAS NSPSVAPYCT RSQRVSENTM LPFVSNRTT FFTRYTPDDW YRSNLTNYQE SNTSRHNSEK LRVDTSRLIQ DKYQQTRKTQ ADTTQNLGER VNDIGFWKSE I IHELDEMI GETNALTDVK KRLERALMET EAPLQVAREC LFHREKRMGI DLVHDEVEAQ LLTEVDTILC CQERMKLHLD KA IAQLAAN RASQHELEKD LSDKQTAYRI DDKCHHLRNT SDGVGYFRGV ERVDATVSVP ESWAKFTDDN ILRSQSERAA SAK LRDDIE NLLVVTANEM WNQFNKVNLS FTNRIAETAD AKNKIQTHLA KTLQEIFQTE MTIESIKKAI KDKTAFLKVA QTRL DERTR RPNIELCRDM AQLRLVNEVH EVDDTIQTLQ QRLRDAEDTL QSLVHIKATL EYDLAVKANS LYIDQEKCMS MRKSY PNTL RLVGFC

Macromolecule #13: Sperm-associated antigen 8

• Macromolecule: Name: Sperm-associated antigen 8 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 51.187598 KDa

Sequence

String:

METNESTEGS RSRSRSLDIQ PSSEGLGPTS EPFPSSDDSP RSALAAATAA AAAAASAAAA TAAFTTAKAA ALSTKTPAPC SEFMEPSSD PSLLGEPCAG PGFTHNIAHG SLGFEPVYVS CIAQDTCTTT DHSSNPGPVP GSSSGPVLGS SSGAGHGSGS G SGPGCGSV PGSGSGPGPG SGPGSGPGHG SGSHPGPASG PGPDTGPDSE LSPCIPPGFR NLVADRVPNY TSWSQHCPWE PQ KQPPWEF LQVLEPGARG LWKPPDIKGK LMVCYETLPR GQCLLYNWEE ERATNHLDQV PSMQDGSESF FFRHGHRGLL TMQ LKSPMP SSTTQKDSYQ PPGNVYWPLR GKREAMLEML LQHQICKEVQ AEQEPTRKLF EVESVTHHDY RMELAQAGTP APTK PHDYR QEQPETFWIQ RAPQLPGVSN IRTLDTPFRK NCSFSTPVPL SLGKLLPYEP ENYPYQLGEI SSLPCPGGRL GGGGG RMTP F

Macromolecule #14: Tektin-4

• Macromolecule: Name: Tektin-4 / type: protein_or_peptide / ID: 14 / Number of copies: 8 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 50.725926 KDa

Sequence

String:

MAQTVPPCEL PCKEYDVARN TGAYTSSGLA TASFRTSKYL LEEWFQNCYA RYHQAFADRD QSERQRHESQ QLATETQALA QRTQQDSTR TVGERLQDTH SWKSELQREM EALAAETNLL LAQKQRLERA LDATEVPFSI TTDNLQCRER REHPNLVRDH V ETELLKEA ELIRNIQELL KRTIMQAVSQ IRLNREHKET CEMDWSDKME AYNIDETCGR HHSQSTEVQA HPYSTTFQES AS TPETRAK FTQDNLCRAQ RERLASANLR VLVDCILRDT SEDLRLQCDA VNLAFGRRCE ELEDARYKLH HHLHKTLREI TDQ EHNVAA LKQAIKDKEA PLHVAQTRLY LRSHRPNMEL CRDAAQFRLL SEVEELNMSL TALREKLLEA EQSLRNLEDI HMSL EKDIA AMTNSLFIDR QKCMAHRTRY PTILQLAGYQ

Macromolecule #15: Cilia- and flagella-associated protein 161

• Macromolecule: Name: Cilia- and flagella-associated protein 161 / type: protein_or_peptide / ID: 15 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 34.339973 KDa

Sequence

String:

MAQNVYGPGV RIGNWNEDVY LEEELMKDFL EKRDKGKLLI QRSRRLKQNL LRPMQLSVTE DGYIHYGDKV MLVNPDDPDT EADVFLRGD LSLCMTPDEI QSHLKDELEV PCGLSAVQAK TPIGRNTFII LSVHRDATGQ VLRYGQDFCL GITGGFDNKM L YLSSDHRT LLKSSKRSWL QEVYLTDEVS HVNCWQAAFP DPQLRLEYEG FPVPANAKIL INHCHTNRGL AAHRHLFLST YF GKEAEVV AHTYLDSHRV EKPRNHWMLV TGNPRDASSS MLDLPKPPTE DTRAMEQAMG LDTQ

Macromolecule #16: Sperm acrosome-associated protein 9

• Macromolecule: Name: Sperm acrosome-associated protein 9 / type: protein_or_peptide / ID: 16 / Number of copies: 21 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 25.208977 KDa

Sequence

String:

MNEVKESLRS IEQKYKLFQQ QQLTFTAALE HCRENAHDKI RPISSIGQVQ SYMEHYCNSS TDRRVLLMFL DICSELNKLC QHFEAVHSG TPVTNNLLEK CKTLVSQSND LSSLRAKYPH DVVNHLSCDE ARNHYGGVVS LIPLILDLMK EWIAHSEKLP R KVLQHVSE PQAHQESTRG AARPAQAIGT QPRATKHKCR QLTKASLKPR GCSKPPWRPP GGKL

Macromolecule #17: Uncharacterized protein C15orf65

• Macromolecule: Name: Uncharacterized protein C15orf65 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 13.778468 KDa

Sequence

String:

MTDRNRDKKS TSPSNSDTEM KSEQLPPCVN PGNPVFSCML DPKTLQTATS LSKPQMIMYK TNSSHYGEFL PIPQFFPCNY TPKEQVFSS HIRATGFYQN NTLNTAPDRT RTLDFPNIQH TL

Macromolecule #18: Protein FAM166B

• Macromolecule: Name: Protein FAM166B / type: protein_or_peptide / ID: 18 / Number of copies: 7 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 30.610637 KDa

Sequence

String:

MAVASTFIPG LNPQNPHYIP GYTGHCPLLR FSVGQTYGQV TGQLLRGPPG LAWPPVHRTL LPPIRPPRSP EVPRESLPVR RGQERLSSS MIPGYTGFVP RAQFIFAKNC SQVWAEALSD FTHLHEKQGS EELPKEAKGR KDTEKDQVPE PEGQLEEPTL E VVEQASPY SMDDRDPRKF FMSGFTGYVP CARFLFGSSF PVLTNQALQE FGQKHSPGSA QDPKHLPPLP RTYPQNLGLL PN YGGYVPG YKFQFGHTFG HLTHDALGLS TFQKQLLA

Macromolecule #19: UPF0686 protein C11orf1

• Macromolecule: Name: UPF0686 protein C11orf1 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 17.806924 KDa

Sequence

String:

MAASQCLCCS KFLFQRQNLA CFLTNPHCGS LVNADGHGEV WTDWNNMSKF FQYGWRCTTN ENTYSNRTLM GNWNQERYDL RNIVQPKPL PSQFGHYFET TYDTSYNNKM PLSTHRFKRE PHWFPGHQPE LDPPRYKCTE KSTYMNSYSK P

Macromolecule #20: Isoform 2 of Cilia- and flagella-associated protein 77

• Macromolecule: Name: Isoform 2 of Cilia- and flagella-associated protein 77; type: protein_or_peptide / ID: 20 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 33.037211 KDa

Sequence

String:

MPEARSSGPD LTRWRKQQQP VRRTVSQVCP PPRRPLTVAD IRSGMENERL GVVRDSMFQN PLIVKAELGK PRERSYSLPG INFNYGLYI RGLDGGVPEA IGRWNVFKQQ PTCPHELTRN YIAMNRGAVK AGLVTARENL LYRQLNDIRI SDQDDRRMKK E PPPLPPNM TFGIRARPST PFFDLLQHRY LQLWVQEQKA TQKAIKLEKK QKVVLGKLYE TRSSQLRKYK PPVKLDTLWH MP HFQKVGR HLDTFPTEAD RQRALKAHRE ECAVRQGTLR MGNYTHP

Macromolecule #21: Protein FAM183A

• Macromolecule: Name: Protein FAM183A / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 16.028189 KDa

Sequence

String:

MAGHPKEKVI PDEVHQNQIL RELYLKELRT QKLYTQYHVN PLRKIHTVTR KPMSWHDNLE EPADARFLNL IHHAAQGPRK KYPETQTEN QEVGWDLEPL INPERHDRRL NHFRVCSDIT LYKAKTWGLG DDHHK

Macromolecule #22: Uncharacterized protein C5orf49

• Macromolecule: Name: Uncharacterized protein C5orf49 / type: protein_or_peptide / ID: 22 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 17.01998 KDa

Sequence

String:

MEDDEEETTA STLRGKPRPP PVSAQSAFSY IPPRRLDPKE HSYYYRPART GIISLYDCIF KRRLDYDQKL HRDDREHAKS LGLHVNEEE QERPVGVLTS SVYGKRINQP IEPLNRDFGR ANHVQADFYR KNDIPSLKEP GFGHIAPS

Macromolecule #23: Protein FAM166C

• Macromolecule: Name: Protein FAM166C / type: protein_or_peptide / ID: 23 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 23.457842 KDa

Sequence

String:

MASRSAGTLL TEFNAAYVPP GLMPGYQGHV PTVAFSFGAP YGTTTLKYFQ DHRNRAMEKS HTPFSQGGHF PTIFSTNPNL LLMERASTR DRWLHKPSYT RFNLDSHRST ELTNFYQMVQ QHRKYYQDKT GTVPRVPYFA MPVREPERYP LPTVLPPLCP K KKWHLLRL APENLKTYQT FPSGKRVSPQ ERKKRDCYFE FRA

Macromolecule #24: RIB43A-like with coiled-coils protein 2

• Macromolecule: Name: RIB43A-like with coiled-coils protein 2 / type: protein_or_peptide / ID: 24 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 45.510234 KDa

Sequence

String:

MGSQTMAVAL PRDLRQDANL AKRRHAELCR QKRVFNARNR IIGGDTEAWD VQVHDQKIKE ATEKARHETF AAEMRQNDKI MCILENRKK RDRKNLCRAI NDFQQSFQKP ETRREFDLSD PLALKKDLPA RQSDNDVRNT ISGMQKFMGE DLNFHERKKF Q EEQNREWS LQQQREWKNA RAEQKCAEAL YTETRLQFDE TAKHLQKLES TTRKAVCASV KDFNKSQAIE SVERKKQEKK QE QEDNLAE ITNLLRGDLL SENPQQAASS FGPHRVVPDR WKGMTQEQLE QIRLVQKQQI QEKLRLQEEK RQRDLDWDRR RIQ GARATL LFERQQWRRQ RDLRRALDSS NLSLAKEQHL QKKYMNEVYT NQPTGDYFTQ FNTGSR

Macromolecule #25: EF-hand domain-containing protein 1

• Macromolecule: Name: EF-hand domain-containing protein 1 / type: protein_or_peptide / ID: 25 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 74.084102 KDa

Sequence

String:

MVSNPVHGLP FLPGTSFKDS TKTAFHRSQT LSYRNGYAIV RRPTVGIGGD RLQFNQLSQA ELDELASKAP VLTYGQPKQA PPADFIPAH VAFDKKVLKF DAYFQEDVPM STEEQYRIRQ VNIYYYLEDD SMSVIEPVVE NSGILQGKLI KRQRLAKNDR G DHYHWKDL NRGINITIYG KTFRVVDCDQ FTQVFLESQG IELNPPEKMA LDPYTELRKQ PLRKYVTPSD FDQLKQFLTF DK QVLRFYA IWDDTDSMYG ECRTYIIHYY LMDDTVEIRE VHERNDGRDP FPLLMNRQRV PKVLVENAKN FPQCVLEISD QEV LEWYTA KDFIVGKSLT ILGRTFFIYD CDPFTRRYYK EKFGITDLPR IDVSKREPPP VKQELPPYNG FGLVEDSAQN CFAL IPKAP KKDVIKMLVN DNKVLRYLAV LESPIPEDKD RRFVFSYFLA TDMISIFEPP VRNSGIIGGK YLGRTKVVKP YSTVD NPVY YGPSDFFIGA VIEVFGHRFI ILDTDEYVLK YMESNAAQYS PEALASIQNH VRKREAPAPE AESKQTEKDP GVQELE ALI DTIQKQLKDH SCKDNIREAF QIYDKEASGY VDRDMFFKIC ESLNVPVDDS LVKELIRMCS HGEGKINYYN FVRAFSN

Macromolecule #26: EF-hand domain-containing family member C2

• Macromolecule: Name: EF-hand domain-containing family member C2 / type: protein_or_peptide / ID: 26 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 87.516828 KDa

Sequence

String:

MALPLLPGNS FNRNVGKEKF HKSQHWGFCN NVMMLVSDEK PGIGGEPLLG QKIKPKCSIY PKGDGSDVPS WVAFDKQVLS FDAYLEEEV LDKSQTNYRI RYYKIYFYPE DDTIQVNEPE VKNSGLLQGT SIRRHRITLP PPDEDQFYTV YHFNVGTEVV F YGRTFKIY DCDAFTRNFL RKIGVKVNPP VQCPEDPYMK IRREVVEHVE PLRPYESLDT LKQFLQYHGK ILCFFCLWDD SV SMFGDRR ELILHYFLCD DTIEIKELLP HSSGRDALKM FLRRSKLPKN CPPRVYQPGQ ITDRAVLNSY GDFIKNQADG YLF DRYKLG KVDQEFYKDS DLSLGVTINV WGRKVLLYDC DEFTKSYYKS KYGIENFTSV SCKPPSPPPK IERKFPPYNG FGSE EDSLR NCIDLKPTPH RRNFKKFMEK DSYGSKSNIL RFFAKLVTDK CVDLDRMFVI SYYLGDDTIS VFEPIERNSG IAGGM FLKR SRVKKPGQEV FKSELSEYIK AEELYIGVTV NVNGYLFRLL NADEYTLNYM EQNTDKYPFS NLKLALQKLK QEEGKS REL KQVFKAADSK HTNMVDYNTF RDILMSLTVG NLAEQEFVTI ARHYRVPEGT CSDMDFLIAL AHEKFKKNMF ENFDTFI YS CVYEDREKKN VLPTKDIKRL CKSSRLPLSD DLLESLLSRF EDSEKQIDYK SFFSALNWRK NPVPELQPAS YLKERCED V WLGMPSPIPA KYIDYWTFLK DAFGLEEE

Macromolecule #27: Cilia- and flagella-associated protein 20

• Macromolecule: Name: Cilia- and flagella-associated protein 20 / type: protein_or_peptide / ID: 27 / Number of copies: 7 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 22.807469 KDa

Sequence

String:

MFKNTFQSGF LSILYSIGSK PLQIWDKKVR NGHIKRITDN DIQSLVLEIE GTNVSTTYIT CPADPKKTLG IKLPFLVMII KNLKKYFTF EVQVLDDKNV RRRFRASNYQ STTRVKPFIC TMPMRLDDGW NQIQFNLLDF TRRAYGTNYI ETLRVQIHAN C RIRRVYFS DRLYSEDELP AEFKLYLPVQ NKAKQ

Macromolecule #28: Parkin coregulated gene protein

• Macromolecule: Name: Parkin coregulated gene protein / type: protein_or_peptide / ID: 28 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 29.352184 KDa

Sequence

String:

MVAEKETLSL NKCPDKMPKR TKLLAQQPLP VHQPHSLVSE GFTVKAMMKN SVVRGPPAAG AFKERPTKPT AFRKFYERGD FPIALEHDS KGNKIAWKVE IEKLDYHHYL PLFFDGLCEM TFPYEFFARQ GIHDMLEHGG NKILPVLPQL IIPIKNALNL R NRQVICVT LKVLQHLVVS AEMVGKALVP YYRQILPVLN IFKNMNVNSG DGIDYSQQKR ENIGDLIQET LEAFERYGGE NA FINIKYV VPTYESCLLN

Macromolecule #29: Cilia- and flagella-associated protein 45

• Macromolecule: Name: Cilia- and flagella-associated protein 45 / type: protein_or_peptide / ID: 29 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 65.858109 KDa

Sequence

String:

MPLSTAGILS SSSAASNRSR NKARYRTKAV SSEVDESLFG DIKSPAQGQS DSPIVLLRDK HTLQKTLTAL GLDRKPETIQ LITRDMVRE LIVPTEDPSG ESLIISPEEF ERIKWASHVL TREELEARDQ AFKKEKEATM DAVMTRKKIM KQKEMVWNNN K KLSDLEEV AKERAQNLLQ RANKLRMEQE EELKDMSKII LNAKCHAIRD AQILEKQQIQ KELDTEEKRL DQMMEVERQK SI QRQEELE RKRREERIRG RRQIVEQMEK NQEERSLLAE QREQEKEQML EYMEQLQEED LKDMERRQQQ KLKMQAEIKR IND ENQKQK AELLAQEKLA DQMVMEFTKK KMAREAEFEA EQERIRREKE KEIARLRAMQ EKAQDYQAEQ DALRAKRNQE VADR EWRRK EKENARKKME TEAELRKSRL EQVAFKEHAL AVQVQRDRDE FERILRAQRE QIEKERLEEE KKATGRLQHA NELRR QVRE NQQKEVQNRI ATFEEGRRLK EEAQKRRERI DEIKRKKLEE LRATGLPEKY CIEAERKANI LPATSVN

Macromolecule #30: Cilia- and flagella-associated protein 52

• Macromolecule: Name: Cilia- and flagella-associated protein 52 / type: protein_or_peptide / ID: 30 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 68.379062 KDa

Sequence

String:

MDNKISPEAQ VAELELDAVI GFNGHVPTGL KCHPDQEHMI YPLGCTVLIQ AINTKEQNFL QGHGNNVSCL AISRSGEYIA SGQVTFMGF KADIILWDYK NRELLARLSL HKGKIEALAF SPNDLYLVSL GGPDDGSVVV WSIAKRDAIC GSPAAGLNVG N ATNVIFSR CRDEMFMTAG NGTIRVWELD LPNRKIWPTE CQTGQLKRIV MSIGVDDDDS FFYLGTTTGD ILKMNPRTKL LT DVGPAKD KFSLGVSAIR CLKMGGLLVG SGAGLLVFCK SPGYKPIKKI QLQGGITSIT LRGEGHQFLV GTEESHIYRV SFT DFKETL IATCHFDAVE DIVFPFGTAE LFATCAKKDI RVWHTSSNRE LLRITVPNMT CHGIDFMRDG KSIISAWNDG KIRA FAPET GRLMYVINNA HRIGVTAIAT TSDCKRVISG GGEGEVRVWQ IGCQTQKLEE ALKEHKSSVS CIRVKRNNEE CVTAS TDGT CIIWDLVRLR RNQMILANTL FQCVCYHPEE FQIITSGTDR KIAYWEVFDG TVIRELEGSL SGSINGMDIT QEGVHF VTG GNDHLVKVWD YNEGEVTHVG VGHSGNITRI RISPGNQYIV SVSADGAILR WKYPYTS

Macromolecule #31: Enkurin

• Macromolecule: Name: Enkurin / type: protein_or_peptide / ID: 31 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 29.510348 KDa

Sequence

String:

MDPTCSSECI YNLIPSDLKE PPQPPRYISI FKATVKDDMQ KAKTAMKTMG PAKVEVPSPK DFLKKHSKEK TLPPKKNFDR NVPKKPAVP LKTDHPVMGI QSGKNFINTN AADIIMGVAK KPKPIYVDKR TGDKHDLEPS GLVPKYINKK DYGVTPEYIC K RNEEIKKA QEDYDRYIQE NLKKAAMKRL SDEEREAVLQ GLKKNWEEVH KEFQSLSVFI DSIPKKIRKQ RLEEEMKQLE HD IGIIEKH KIIYIANNA

Macromolecule #32: Protein Flattop

• Macromolecule: Name: Protein Flattop / type: protein_or_peptide / ID: 32 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 19.320871 KDa

Sequence

String:

MATNYSANQY EKAFSSKYLQ NWSPTKPTKE SISSHEGYTQ IIANDRGHLL PSVPRSKANP WGSFMGTWQM PLKIPPARVT LTSRTTAGA ASLTKWIQKN PDLLKASNGL CPEILGKPHD PDSQKKLRKK SITKTVQQAR SPTIIPSSPA ANLNSPDELQ S SHPSAGHT PGPQRPAKS

Macromolecule #33: Protein CFAP210

• Macromolecule: Name: Protein CFAP210 / type: protein_or_peptide / ID: 33 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 66.544008 KDa

Sequence

String:

MDTSSEMLVR FGRRCGRAKE STEIRNSEED QVLYLPLLPS KVDLQQVTII PHDEWKRIQD SLDRLTREAA CLRAERKAKK EMHLRSQEV VKHWTNTYAG MKEQKLEAKK KRDEEIEAER QILDLEEEIY KQGKRKKAIE NAKQYQFYQT ERVKNFHSGL L LSRVMKER DAQIEFRKSK IKSDKKWEEQ LKLNIEKAFK EEQEKAEKRH RERVALAKDH LKQIKEHEEE EERRKKYEEK DA EEIKRQN ALYEIEMRKK LEKKREEMHE SRRRFLEHMQ DKHIIKAVEQ QQQEEEDEKM RKFIKAKKRL IQMGKEKEAE THR LMEKRR ERIHNFLSEL LKEKLDNEDM IIARDIAEAE AEWEKREREK DEKNKAELKT IAEYRAIVMK NKEEEERQRK IEAK EQLLA VMKADQIFWE HEKEKKCKAD KEHQEVQDAH IQQMAKNKFN AKQAKQAELD YCRLTEALVA EKEKEFQDYA REVIE LESE TTNKYIYPLV KAVQEGPGGG RGPVFVDRGG LRPSYQANDV TGVQLPFYNS QGPKYNFQKS KRRLGFTW

Macromolecule #34: Protein CFAP276

• Macromolecule: Name: Protein CFAP276 / type: protein_or_peptide / ID: 34 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 19.376688 KDa

Sequence

String:

MPPTRDPFQQ PTLDNDDSYL GELRASKKLP YKNPTHLAQQ QEPWSRLNST PTITSMRRDA YYFDPEIPKD DLDFRLAALY NHHTGTFKN KSEILLNQKT TQDTYRTKIQ FPGEFLTPPT PPITFLANIR HWINPKKESI HSIQGSIVSP HTAATNGGYS R KKDGGFFS T

Macromolecule #35: UPF0691 protein C9orf116

• Macromolecule: Name: UPF0691 protein C9orf116 / type: protein_or_peptide / ID: 35 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 15.278101 KDa

Sequence

String:

MAEECPRACA EPVAPKATAP PERTSDYYRV SADLPGRFNN PGWFRGYRTQ KAVSVYRTSN QAYGSRAPTV HEMPKVFYPN SNKFSQQLA AGGMFRNNTL NVYLEKSIVT GPDNCITSCD RLNFHPSYNI NRPSICD

Macromolecule #36: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 36 / Number of copies: 149 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

Macromolecule #37: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 37 / Number of copies: 149 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #38: GUANOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 38 / Number of copies: 153 / Formula: GDP
• Molecular weight: Theoretical: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: filament

Sample preparation

• Buffer: pH: 7.3
• Grid: Model: Quantifoil R2/1 / Pretreatment - Type: GLOW DISCHARGE
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 %

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.8 µm / Nominal magnification: 64000
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 60.0 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

Startup model

Type of model: EMDB MAP
EMDB ID:

24664

• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 208558