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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-21942 | |||||||||||||||||||||||||||||||||
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Title | KIF14[391-748] - AMP-PNP in complex with a microtubule | |||||||||||||||||||||||||||||||||
![]() | Main map. Locally refined single unit. Not helically averaged. | |||||||||||||||||||||||||||||||||
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Function / homology | ![]() cerebellar granular layer structural organization / regulation of cell maturation / cerebellar Purkinje cell layer structural organization / RHO GTPases activate CIT / RND2 GTPase cycle / negative regulation of integrin activation / RND1 GTPase cycle / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||||||||||||||||||||||||||
Biological species | ![]() ![]() ![]() ![]() ![]() ![]() | |||||||||||||||||||||||||||||||||
Method | helical reconstruction / ![]() | |||||||||||||||||||||||||||||||||
![]() | Benoit MPMH / Asenjo AB | |||||||||||||||||||||||||||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: Structural basis of mechano-chemical coupling by the mitotic kinesin KIF14. Authors: Matthieu P M H Benoit / Ana B Asenjo / Mohammadjavad Paydar / Sabin Dhakal / Benjamin H Kwok / Hernando Sosa / ![]() ![]() Abstract: KIF14 is a mitotic kinesin whose malfunction is associated with cerebral and renal developmental defects and several cancers. Like other kinesins, KIF14 couples ATP hydrolysis and microtubule binding ...KIF14 is a mitotic kinesin whose malfunction is associated with cerebral and renal developmental defects and several cancers. Like other kinesins, KIF14 couples ATP hydrolysis and microtubule binding to the generation of mechanical work, but the coupling mechanism between these processes is still not fully clear. Here we report 20 high-resolution (2.7-3.9 Å) cryo-electron microscopy KIF14-microtubule structures with complementary functional assays. Analysis procedures were implemented to separate coexisting conformations of microtubule-bound monomeric and dimeric KIF14 constructs. The data provide a comprehensive view of the microtubule and nucleotide induced KIF14 conformational changes. It shows that: 1) microtubule binding, the nucleotide species, and the neck-linker domain govern the transition between three major conformations of the motor domain; 2) an undocked neck-linker prevents the nucleotide-binding pocket to fully close and dampens ATP hydrolysis; 3) 13 neck-linker residues are required to assume a stable docked conformation; 4) the neck-linker position controls the hydrolysis rather than the nucleotide binding step; 5) the two motor domains of KIF14 dimers adopt distinct conformations when bound to the microtubule; and 6) the formation of the two-heads-bound-state introduces structural changes in both motor domains of KIF14 dimers. These observations provide the structural basis for a coordinated chemo-mechanical kinesin translocation model. | |||||||||||||||||||||||||||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 6.9 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 34.9 KB 34.9 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 14.7 KB | Display | ![]() |
Images | ![]() | 155.9 KB | ||
Masks | ![]() ![]() ![]() ![]() ![]() ![]() | 1.1 GB 274.6 MB 1.1 GB 1.1 GB 274.6 MB 274.6 MB | ![]() | |
Filedesc metadata | ![]() | 8.3 KB | ||
Others | ![]() ![]() ![]() ![]() ![]() | 836.1 MB 836 MB 204.4 MB 218.5 MB 218.4 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6wwoMC ![]() 6wweC ![]() 6wwfC ![]() 6wwgC ![]() 6wwhC ![]() 6wwiC ![]() 6wwjC ![]() 6wwkC ![]() 6wwlC ![]() 6wwmC ![]() 6wwnC ![]() 6wwpC ![]() 6wwqC ![]() 6wwrC ![]() 6wwsC ![]() 6wwtC ![]() 6wwuC ![]() 6wwvC ![]() 7lvqC ![]() 7lvrC C: citing same article ( M: atomic model generated by this map |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | Main map. Locally refined single unit. Not helically averaged. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.828 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
+Mask #1
+Mask #2
+Mask #3
+Mask #4
+Mask #5
+Mask #6
+Additional map: Helical reconstruction half map 2 (gold standard).
+Additional map: Helical reconstruction half map 1 (gold standard).
+Additional map: Helical reconstruction.
+Half map: Half map 2 (gold standard). Locally refined single...
+Half map: Half map 1 (gold standard). Locally refined single...
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Sample components
+Entire : Kif14[391-748] - AMP-PNP in complex with a microtubule
+Supramolecule #1: Kif14[391-748] - AMP-PNP in complex with a microtubule
+Supramolecule #2: KIF14[391-748] - AMP-PNP
+Supramolecule #3: Microtubule
+Macromolecule #1: Tubulin alpha-1B chain
+Macromolecule #2: Tubulin beta-2B chain
+Macromolecule #3: Kinesin-like protein KIF14
+Macromolecule #4: GUANOSINE-5'-TRIPHOSPHATE
+Macromolecule #5: MAGNESIUM ION
+Macromolecule #6: GUANOSINE-5'-DIPHOSPHATE
+Macromolecule #7: TAXOL
+Macromolecule #8: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
-Experimental details
-Structure determination
Method | ![]() |
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![]() | helical reconstruction |
Aggregation state | filament |
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Sample preparation
Buffer | pH: 6.8 Component:
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Grid | Model: UltrAuFoil / Mesh: 200 / Support film - Material: GOLD / Support film - topology: HOLEY / Pretreatment - Type: PLASMA CLEANING | ||||||||||||
Vitrification | Cryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Calibrated magnification: 60386 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Digitization - Frames/image: 1-40 / Average electron dose: 68.2 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
-Atomic model buiding 1
Refinement | Space: REAL / Protocol: FLEXIBLE FIT |
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Output model | ![]() PDB-6wwo: |