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基本情報
登録情報 | ![]() | |||||||||
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タイトル | Open Complex I from murine liver | |||||||||
![]() | combined map | |||||||||
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機能・相同性 | ![]() response to injury involved in regulation of muscle adaptation / Mitochondrial Fatty Acid Beta-Oxidation / Complex I biogenesis / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() ![]() | |||||||||
手法 | ![]() ![]() | |||||||||
![]() | Vercellino I / Sazanov LA | |||||||||
資金援助 | European Union, 1件
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![]() | ![]() タイトル: SCAF1 drives the compositional diversity of mammalian respirasomes. 著者: Irene Vercellino / Leonid A Sazanov / ![]() ![]() 要旨: Supercomplexes of the respiratory chain are established constituents of the oxidative phosphorylation system, but their role in mammalian metabolism has been hotly debated. Although recent studies ...Supercomplexes of the respiratory chain are established constituents of the oxidative phosphorylation system, but their role in mammalian metabolism has been hotly debated. Although recent studies have shown that different tissues/organs are equipped with specific sets of supercomplexes, depending on their metabolic needs, the notion that supercomplexes have a role in the regulation of metabolism has been challenged. However, irrespective of the mechanistic conclusions, the composition of various high molecular weight supercomplexes remains uncertain. Here, using cryogenic electron microscopy, we demonstrate that mammalian (mouse) tissues contain three defined types of 'respirasome', supercomplexes made of CI, CIII and CIV. The stoichiometry and position of CIV differs in the three respirasomes, of which only one contains the supercomplex-associated factor SCAF1, whose involvement in respirasome formation has long been contended. Our structures confirm that the 'canonical' respirasome (the C-respirasome, CICIIICIV) does not contain SCAF1, which is instead associated to a different respirasome (the CS-respirasome), containing a second copy of CIV. We also identify an alternative respirasome (A-respirasome), with CIV bound to the 'back' of CI, instead of the 'toe'. This structural characterization of mouse mitochondrial supercomplexes allows us to hypothesize a mechanistic basis for their specific role in different metabolic conditions. #1: ![]() 年: 2018 タイトル: Real-space refinement in PHENIX for cryo-EM and crystallography 著者: Afonine PV / Adams PD | |||||||||
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構造の表示
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-EMDBアーカイブ
マップデータ | ![]() | 6.6 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 66.8 KB 66.8 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 21.1 KB | 表示 | ![]() |
画像 | ![]() | 93.8 KB | ||
Filedesc metadata | ![]() | 14.6 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 8rgqMC ![]() 8pw5C ![]() 8pw6C ![]() 8pw7C ![]() 8rgpC ![]() 8rgrC ![]() 8rgtC C: 同じ文献を引用 ( M: このマップから作成された原子モデル |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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注釈 | combined map | ||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.06 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : Open Complex I from murine liver
+超分子 #1: Open Complex I from murine liver
+分子 #1: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
+分子 #2: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
+分子 #3: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
+分子 #4: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
+分子 #5: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
+分子 #6: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
+分子 #7: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
+分子 #8: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mit...
+分子 #9: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
+分子 #10: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
+分子 #11: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
+分子 #12: Acyl carrier protein, mitochondrial
+分子 #13: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
+分子 #14: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
+分子 #15: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
+分子 #16: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
+分子 #17: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial
+分子 #18: NADH-ubiquinone oxidoreductase chain 3
+分子 #19: NADH-ubiquinone oxidoreductase chain 1
+分子 #20: NADH-ubiquinone oxidoreductase chain 6
+分子 #21: NADH-ubiquinone oxidoreductase chain 4L
+分子 #22: NADH-ubiquinone oxidoreductase chain 5
+分子 #23: NADH-ubiquinone oxidoreductase chain 4
+分子 #24: NADH-ubiquinone oxidoreductase chain 2
+分子 #25: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mi...
+分子 #26: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
+分子 #27: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11
+分子 #28: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
+分子 #29: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
+分子 #30: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
+分子 #31: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial
+分子 #32: NADH dehydrogenase [ubiquinone] 1 subunit C2
+分子 #33: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
+分子 #34: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1
+分子 #35: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mit...
+分子 #36: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mito...
+分子 #37: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
+分子 #38: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mito...
+分子 #39: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
+分子 #40: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mito...
+分子 #41: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
+分子 #42: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
+分子 #43: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
+分子 #44: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
+分子 #45: IRON/SULFUR CLUSTER
+分子 #46: 1,2-Distearoyl-sn-glycerophosphoethanolamine
+分子 #47: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
+分子 #48: FE2/S2 (INORGANIC) CLUSTER
+分子 #49: FLAVIN MONONUCLEOTIDE
+分子 #50: POTASSIUM ION
+分子 #51: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
+分子 #52: ZINC ION
+分子 #53: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-b...
+分子 #54: CARDIOLIPIN
+分子 #55: 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE
+分子 #56: MAGNESIUM ION
-実験情報
-構造解析
手法 | ![]() |
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試料の集合状態 | particle |
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試料調製
濃度 | 0.3 mg/mL | ||||||||||||
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緩衝液 | pH: 7.7 構成要素:
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グリッド | モデル: Quantifoil R0.6/1 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: CONTINUOUS / 支持フィルム - Film thickness: 1 | ||||||||||||
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277.15 K / 装置: FEI VITROBOT MARK IV |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | C2レンズ絞り径: 50.0 µm / 最大 デフォーカス(補正後): 2.5 µm / 最小 デフォーカス(補正後): 0.5 µm / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD![]() |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER ホルダー冷却材: NITROGEN |
撮影 | フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) 撮影したグリッド数: 1 / 実像数: 9453 / 平均露光時間: 4.4 sec. / 平均電子線量: 80.0 e/Å2 |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |