- EMDB-10549: The structure of ABC transporter Rv1819c without addition of substrate -
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Basic information
Entry
Database: EMDB / ID: EMD-10549
Title
The structure of ABC transporter Rv1819c without addition of substrate
Map data
Sample
Complex: dimer of Rv1819c
Protein or peptide: ABC transporter ATP-binding protein/permease
Ligand: ADENOSINE-5'-TRIPHOSPHATE
Ligand: MAGNESIUM ION
Keywords
cobalamin / vitamin B12 / ABC transporter / exporter fold / import / tuberculosis / TRANSPORT PROTEIN
Function / homology
Function and homology information
response to host immune response / Translocases; Catalysing the translocation of other compounds; Linked to the hydrolysis of a nucleoside triphosphate / ATPase-coupled transmembrane transporter activity / ABC-type transporter activity / ATP hydrolysis activity / extracellular region / ATP binding / membrane / plasma membrane Similarity search - Function
ABC transporter transmembrane region 2 / ABC transporter type 1, transmembrane domain / ABC transporter integral membrane type-1 fused domain profile. / ABC transporter type 1, transmembrane domain superfamily / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain / ATP-binding cassette, ABC transporter-type domain profile. / ATPases associated with a variety of cellular activities ...ABC transporter transmembrane region 2 / ABC transporter type 1, transmembrane domain / ABC transporter integral membrane type-1 fused domain profile. / ABC transporter type 1, transmembrane domain superfamily / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain / ATP-binding cassette, ABC transporter-type domain profile. / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase Similarity search - Domain/homology
Multidrug ABC transporter ATP-binding protein / Hydrophilic compounds import ATP-binding/permease protein BacA Similarity search - Component
Biological species
Mycobacterium tuberculosis (bacteria)
Method
single particle reconstruction / cryo EM / Resolution: 4.3 Å
Journal: Nature / Year: 2020 Title: A mycobacterial ABC transporter mediates the uptake of hydrophilic compounds. Authors: S Rempel / C Gati / M Nijland / C Thangaratnarajah / A Karyolaimos / J W de Gier / A Guskov / D J Slotboom / Abstract: Mycobacterium tuberculosis (Mtb) is an obligate human pathogen and the causative agent of tuberculosis. Although Mtb can synthesize vitamin B (cobalamin) de novo, uptake of cobalamin has been linked ...Mycobacterium tuberculosis (Mtb) is an obligate human pathogen and the causative agent of tuberculosis. Although Mtb can synthesize vitamin B (cobalamin) de novo, uptake of cobalamin has been linked to pathogenesis of tuberculosis. Mtb does not encode any characterized cobalamin transporter; however, the gene rv1819c was found to be essential for uptake of cobalamin. This result is difficult to reconcile with the original annotation of Rv1819c as a protein implicated in the transport of antimicrobial peptides such as bleomycin. In addition, uptake of cobalamin seems inconsistent with the amino acid sequence, which suggests that Rv1819c has a bacterial ATP-binding cassette (ABC)-exporter fold. Here, we present structures of Rv1819c, which reveal that the protein indeed contains the ABC-exporter fold, as well as a large water-filled cavity of about 7,700 Å, which enables the protein to transport the unrelated hydrophilic compounds bleomycin and cobalamin. On the basis of these structures, we propose that Rv1819c is a multi-solute transporter for hydrophilic molecules, analogous to the multidrug exporters of the ABC transporter family, which pump out structurally diverse hydrophobic compounds from cells.
History
Deposition
Dec 16, 2019
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Header (metadata) release
Jan 29, 2020
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Map release
Apr 1, 2020
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Update
May 22, 2024
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Current status
May 22, 2024
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
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