+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-0491 | ||||||||||||||||||||||||
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タイトル | hTRiC-hPFD Class5 | ||||||||||||||||||||||||
マップデータ | hTRiC-hPFD Class5 | ||||||||||||||||||||||||
試料 |
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キーワード | TRiC/CCT / PFD / CryoEM / Molecular Chaperone / Protein folding / CHAPERONE | ||||||||||||||||||||||||
機能・相同性 | 機能・相同性情報 RNA polymerase I assembly / RNA polymerase III assembly / prefoldin complex / positive regulation of cytoskeleton organization / zona pellucida receptor complex / scaRNA localization to Cajal body / chaperone mediated protein folding independent of cofactor / positive regulation of establishment of protein localization to telomere / positive regulation of protein localization to Cajal body / tubulin complex assembly ...RNA polymerase I assembly / RNA polymerase III assembly / prefoldin complex / positive regulation of cytoskeleton organization / zona pellucida receptor complex / scaRNA localization to Cajal body / chaperone mediated protein folding independent of cofactor / positive regulation of establishment of protein localization to telomere / positive regulation of protein localization to Cajal body / tubulin complex assembly / RNA polymerase II core complex assembly / BBSome-mediated cargo-targeting to cilium / RPAP3/R2TP/prefoldin-like complex / binding of sperm to zona pellucida / Folding of actin by CCT/TriC / Formation of tubulin folding intermediates by CCT/TriC / positive regulation of telomerase RNA localization to Cajal body / chaperonin-containing T-complex / Prefoldin mediated transfer of substrate to CCT/TriC / negative regulation of amyloid fibril formation / protein folding chaperone complex / RHOBTB1 GTPase cycle / intermediate filament cytoskeleton / WD40-repeat domain binding / pericentriolar material / beta-tubulin binding / Association of TriC/CCT with target proteins during biosynthesis / : / microtubule-based process / chaperone-mediated protein complex assembly / RHOBTB2 GTPase cycle / heterochromatin / chaperone-mediated protein folding / protein folding chaperone / positive regulation of telomere maintenance via telomerase / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / tubulin binding / acrosomal vesicle / cell projection / mRNA 3'-UTR binding / ATP-dependent protein folding chaperone / response to virus / negative regulation of canonical Wnt signaling pathway / cilium / mRNA 5'-UTR binding / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / transcription corepressor activity / G-protein beta-subunit binding / azurophil granule lumen / unfolded protein binding / melanosome / protein folding / retina development in camera-type eye / amyloid-beta binding / protein-folding chaperone binding / cell body / secretory granule lumen / ficolin-1-rich granule lumen / microtubule / cytoskeleton / protein stabilization / cadherin binding / intracellular membrane-bounded organelle / negative regulation of DNA-templated transcription / centrosome / ubiquitin protein ligase binding / Neutrophil degranulation / regulation of DNA-templated transcription / Golgi apparatus / ATP hydrolysis activity / mitochondrion / RNA binding / extracellular exosome / extracellular region / nucleoplasm / ATP binding / nucleus / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||||||||||||||||||||
生物種 | Homo sapiens (ヒト) | ||||||||||||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 8.5 Å | ||||||||||||||||||||||||
データ登録者 | Gestaut D / Roh SH | ||||||||||||||||||||||||
資金援助 | 米国, European Union, 7件
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引用 | ジャーナル: Cell / 年: 2019 タイトル: The Chaperonin TRiC/CCT Associates with Prefoldin through a Conserved Electrostatic Interface Essential for Cellular Proteostasis. 著者: Daniel Gestaut / Soung Hun Roh / Boxue Ma / Grigore Pintilie / Lukasz A Joachimiak / Alexander Leitner / Thomas Walzthoeni / Ruedi Aebersold / Wah Chiu / Judith Frydman / 要旨: Maintaining proteostasis in eukaryotic protein folding involves cooperation of distinct chaperone systems. To understand how the essential ring-shaped chaperonin TRiC/CCT cooperates with the ...Maintaining proteostasis in eukaryotic protein folding involves cooperation of distinct chaperone systems. To understand how the essential ring-shaped chaperonin TRiC/CCT cooperates with the chaperone prefoldin/GIMc (PFD), we integrate cryoelectron microscopy (cryo-EM), crosslinking-mass-spectrometry and biochemical and cellular approaches to elucidate the structural and functional interplay between TRiC/CCT and PFD. We find these hetero-oligomeric chaperones associate in a defined architecture, through a conserved interface of electrostatic contacts that serves as a pivot point for a TRiC-PFD conformational cycle. PFD alternates between an open "latched" conformation and a closed "engaged" conformation that aligns the PFD-TRiC substrate binding chambers. PFD can act after TRiC bound its substrates to enhance the rate and yield of the folding reaction, suppressing non-productive reaction cycles. Disrupting the TRiC-PFD interaction in vivo is strongly deleterious, leading to accumulation of amyloid aggregates. The supra-chaperone assembly formed by PFD and TRiC is essential to prevent toxic conformations and ensure effective cellular proteostasis. | ||||||||||||||||||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_0491.map.gz | 48.8 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-0491-v30.xml emd-0491.xml | 38.9 KB 38.9 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_0491_fsc.xml | 8.7 KB | 表示 | FSCデータファイル |
画像 | emd_0491.png | 123.8 KB | ||
Filedesc metadata | emd-0491.cif.gz | 9.3 KB | ||
その他 | emd_0491_additional.map.gz emd_0491_half_map_1.map.gz emd_0491_half_map_2.map.gz | 40.8 MB 40.9 MB 40.9 MB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-0491 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-0491 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_0491_validation.pdf.gz | 963.4 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_0491_full_validation.pdf.gz | 963 KB | 表示 | |
XML形式データ | emd_0491_validation.xml.gz | 14.3 KB | 表示 | |
CIF形式データ | emd_0491_validation.cif.gz | 20.3 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-0491 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-0491 | HTTPS FTP |
-関連構造データ
関連構造データ | 6nr9MC 0490C 0492C 0493C 0494C 0495C 0496C 6nr8C 6nraC 6nrbC 6nrcC 6nrdC C: 同じ文献を引用 (文献) M: このマップから作成された原子モデル |
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類似構造データ |
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_0491.map.gz / 形式: CCP4 / 大きさ: 52.7 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | hTRiC-hPFD Class5 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.7 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-追加マップ: hTRiC-hPFD Class5, additional map
ファイル | emd_0491_additional.map | ||||||||||||
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注釈 | hTRiC-hPFD Class5, additional map | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: hTRiC-hPFD Class5, half map 1
ファイル | emd_0491_half_map_1.map | ||||||||||||
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注釈 | hTRiC-hPFD Class5, half map 1 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: hTRiC-hPFD Class5, half map 2
ファイル | emd_0491_half_map_2.map | ||||||||||||
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注釈 | hTRiC-hPFD Class5, half map 2 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-試料の構成要素
+全体 : hTRiC-hPFD
+超分子 #1: hTRiC-hPFD
+超分子 #2: hTRiC
+超分子 #3: hPFD
+分子 #1: T-complex protein 1 subunit alpha
+分子 #2: T-complex protein 1 subunit beta
+分子 #3: T-complex protein 1 subunit gamma
+分子 #4: T-complex protein 1 subunit delta
+分子 #5: T-complex protein 1 subunit epsilon
+分子 #6: T-complex protein 1 subunit zeta
+分子 #7: T-complex protein 1 subunit eta
+分子 #8: T-complex protein 1 subunit theta
+分子 #9: Prefoldin subunit 1
+分子 #10: Prefoldin subunit 2
+分子 #11: Prefoldin subunit 3
+分子 #12: Prefoldin subunit 4
+分子 #13: Prefoldin subunit 5
+分子 #14: Prefoldin subunit 6
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.5 |
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グリッド | 詳細: unspecified |
凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | JEOL 3200FSC |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 1.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |