グアノシン三リン酸 / リボ核酸 / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein uS4A / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS11A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A ...グアノシン三リン酸 / リボ核酸 / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein uS4A / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS11A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A / Small ribosomal subunit protein eS4A / Small ribosomal subunit protein eS6A / Small ribosomal subunit protein eS8A / Small ribosomal subunit protein uS17A / Small ribosomal subunit protein uS9A / rRNA 2'-O-methyltransferase fibrillarin / Exosome complex component RRP43 / Ribosomal RNA-processing protein 7 / Periodic tryptophan protein 2 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS7A / U3 small nucleolar ribonucleoprotein protein IMP3 / Small ribosomal subunit protein eS1A / Protein SOF1 / U3 small nucleolar RNA-associated protein 11 / U3 small nucleolar RNA-associated protein 20 / Small ribosomal subunit protein eS27A / Essential nuclear protein 1 / Exosome complex component RRP4 / Exosome complex protein LRP1 / U3 small nucleolar RNA-associated protein 9 / 13 kDa ribonucleoprotein-associated protein / U3 small nucleolar RNA-associated protein 7 / U3 small nucleolar RNA-associated protein 18 / Regulator of rDNA transcription protein 14 / U3 small nucleolar RNA-associated protein 10 / Exosome complex component SKI6 / ATP-dependent RNA helicase DOB1 / U3 small nucleolar RNA-associated protein MPP10 / Exosome complex component MTR3 / U3 small nucleolar RNA-associated protein 22 / Exosome complex component RRP46 / U3 small nucleolar RNA-associated protein 8 / M-phase phosphoprotein 6 homolog / Exosome complex component CSL4 / U3 small nucleolar ribonucleoprotein protein IMP4 / U3 small nucleolar RNA-associated protein 6 / NET1-associated nuclear protein 1 / U3 small nucleolar RNA-associated protein 5 / U3 small nucleolar RNA-associated protein 15 / U3 small nucleolar RNA-associated protein 14 / rRNA biogenesis protein RRP5 / rRNA-processing protein FCF1 / Exosome complex component RRP45 / U3 small nucleolar RNA-associated protein 13 / U3 small nucleolar RNA-associated protein 21 / Ribosomal RNA small subunit methyltransferase NEP1 / Ribosomal RNA-processing protein 9 / Nucleolar complex protein 4 / U3 small nucleolar RNA-associated protein 4 / RNA 3'-terminal phosphate cyclase-like protein / Exosome complex exonuclease DIS3 / Exosome complex component RRP40 / U3 small nucleolar RNA-associated protein 16 / Ribosome biogenesis protein BMS1 / rRNA-processing protein FCF2 / Something about silencing protein 10 / Exosome complex exonuclease RRP6 / U3 small nucleolar RNA-associated protein 12 / Exosome complex component RRP42 / Nucleolar protein 56 / Nucleolar protein 58 / Small ribosomal subunit protein eS28A / Small ribosomal subunit protein uS8B / Nucleolar complex protein 14 類似検索 - 構成要素
National Natural Science Foundation of China (NSFC)
91940302, 91540201, 31430024, 31325007
中国
Chinese Academy of Sciences
XDB08010203
中国
引用
ジャーナル: Science / 年: 2020 タイトル: Cryo-EM structure of 90 small ribosomal subunit precursors in transition states. 著者: Yifei Du / Weidong An / Xing Zhu / Qi Sun / Jia Qi / Keqiong Ye / 要旨: The 90 preribosome is a large, early assembly intermediate of small ribosomal subunits that undergoes structural changes to give a pre-40 ribosome. Here, we gained insight into this transition by ...The 90 preribosome is a large, early assembly intermediate of small ribosomal subunits that undergoes structural changes to give a pre-40 ribosome. Here, we gained insight into this transition by determining cryo-electron microscopy structures of intermediates in the path from the 90 to the pre-40 The full transition is blocked by deletion of RNA helicase Dhr1. A series of structural snapshots revealed that the excised 5' external transcribed spacer (5' ETS) is degraded within 90, driving stepwise disassembly of assembly factors and ribosome maturation. The nuclear exosome, an RNA degradation machine, docks on the 90 through helicase Mtr4 and is primed to digest the 3' end of the 5' ETS. The structures resolved between 3.2- and 8.6-angstrom resolution reveal key intermediates and the critical role of 5' ETS degradation in 90 progression.
NET1-associatednuclearprotein1 / Utp17 / U three protein 17 / t-17 / U3 protein 17 required for transcription / U3 small nucleolar ...Utp17 / U three protein 17 / t-17 / U3 protein 17 required for transcription / U3 small nucleolar RNA-associated protein 17 / U3 snoRNA-associated protein 17
rRNAbiogenesisproteinRRP5 / Ribosomal RNA-processing protein 5 / U3 small nucleolar RNA-associated protein RRP5 / U3 snoRNA- ...Ribosomal RNA-processing protein 5 / U3 small nucleolar RNA-associated protein RRP5 / U3 snoRNA-associated protein RRP5
U3smallnucleolarRNA-associatedprotein10 / Utp10 / U3 snoRNA-associated protein 10 / U three protein 10 / U3 protein 10 required for ...Utp10 / U3 snoRNA-associated protein 10 / U three protein 10 / U3 protein 10 required for transcription / t-UTP10
U3smallnucleolarRNA-associatedprotein15 / Utp15 / U3 snoRNA-associated protein 15 / U three protein 15 / U3 protein 15 required for ...Utp15 / U3 snoRNA-associated protein 15 / U three protein 15 / U3 protein 15 required for transcription / t-UTP15