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    - PDB-3izy: Mammalian mitochondrial translation initiation factor 2 -

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    Basic information

    Entry
    Database: PDB / ID: 3izy
    TitleMammalian mitochondrial translation initiation factor 2
    DescriptorProtein/RNA complex
    KeywordsRNA / RIBOSOMAL PROTEIN / translation initiation factor 2 / IF2 / E coli
    Specimen sourceBos taurus / mammal / cow /
    MethodElectron microscopy (10.8 A resolution / Single particle / Vitreous ice (cryo EM))
    AuthorsYassin, A.S. / Haque, E. / Datta, P.P. / Elmore, K. / Banavali, N.K. / Spremulli, L.L. / Agrawal, R.K.
    CitationProc. Natl. Acad. Sci. U.S.A., 2011, 108, 3918-3923

    Proc. Natl. Acad. Sci. U.S.A., 2011, 108, 3918-3923 StrPapers
    Insertion domain within mammalian mitochondrial translation initiation factor 2 serves the role of eubacterial initiation factor 1.
    Aymen S Yassin / Md Emdadul Haque / Partha P Datta / Kevin Elmore / Nilesh K Banavali / Linda L Spremulli / Rajendra K Agrawal

    DateDeposition: Jan 19, 2011 / Release: Mar 23, 2011 / Last modification: Jan 21, 2015

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    Assembly

    Deposited unit
    N: tRNA-Phe
    P: Translation initiation factor IF-2, mitochondrial

    84.1 kDa, 2 molecules
    Theoretical massNumber of molelcules
    Total
    (without water)
    84,1222
    Polyers84,1222
    Non-polymers00
    Water0

    Omokage search
    #1idetical with deposited unit / defined by author / Symmetry operations: (identity)x1
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    Components

    #1RNA chain / tRNA-Phe / Source: Bos taurus (gene. exp.)
    #2polypeptide(L) / Translation initiation factor IF-2, mitochondrial / IF-2(Mt), IF-2Mt, IF2(mt) / Source: Bos taurus (gene. exp.) / References: UniProt: P46198

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    Experimental details

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    Experiment

    ExperimentMethod: ELECTRON MICROSCOPY
    EM experimentReconstruction method: SINGLE PARTICLE / Specimen type: VITREOUS ICE (CRYO EM)

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    Sample preparation

    Assembly of specimenName: Mammalian mitochondrial translation initiation factor 2 interaction with tRNA
    Aggregation state: PARTICLE

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    Electron microscopy imaging

    MicroscopyMicroscope model: Tecnai F20
    Electron gunElectron source: FEG / Accelerating voltage: 200 kV
    Electron lensNominal magnification: 50000 X / Calibrated magnification: 50760 X
    CameraType: Kodak S0163
    RadiationDiffraction protocol: SINGLE WAVELENGTH / Monochromatic or laue m l: M / Scattering type: x-ray
    Radiation wavelengthRelative weight: 1

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    Processing

    EM single particle entitySymmetry type: ASYMMETRIC
    3D reconstructionMethod: Back Projection / Software: Spider / Resolution: 10.8 A / Number of particles: 121742 / Actual pixel size: 2.76 A/pix / CTF correction method: Every Micrograph
    Number of atoms included #LASTProtein: 4180 / Nucleic acid: 1622 / Ligand: 0 / Solvent: 0 / Total: 5802

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