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- PDB-3izy: Mammalian mitochondrial translation initiation factor 2 -

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Basic information

Entry
Database: PDB / ID: 3izy
TitleMammalian mitochondrial translation initiation factor 2
DescriptorPROTEIN/RNA Complex
KeywordsRNA / RIBOSOMAL PROTEIN / translation initiation factor 2 / IF2 / E coli
Specimen sourceBos taurus / mammal / cow / ウシ /
MethodElectron microscopy (10.8 A resolution / Single particle / Vitreous ice (cryo EM))
AuthorsYassin, A.S. / Haque, E. / Datta, P.P. / Elmore, K. / Banavali, N.K. / Spremulli, L.L. / Agrawal, R.K.
CitationProc. Natl. Acad. Sci. U.S.A., 2011, 108, 3918-3923

Proc. Natl. Acad. Sci. U.S.A., 2011, 108, 3918-3923 StrPapers
Insertion domain within mammalian mitochondrial translation initiation factor 2 serves the role of eubacterial initiation factor 1.
Aymen S Yassin / Md Emdadul Haque / Partha P Datta / Kevin Elmore / Nilesh K Banavali / Linda L Spremulli / Rajendra K Agrawal

DateDeposition: Jan 19, 2011 / Release: Mar 23, 2011 / Last modification: Jan 21, 2015

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Assembly

Deposited unit
N: tRNA-Phe
P: Translation initiation factor IF-2, mitochondrial


Theoretical massNumber of molelcules
Total (without water)84,1222
Polyers84,1222
Non-polymers00
Water0
#1


TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

#1: RNA chaintRNA-Phe


Mass: 24518.730 Da / Num. of mol.: 1 / Source: (natural) Bos taurus / mammal / ウシ /
#2: Polypeptide(L)Translation initiation factor IF-2, mitochondrial / IF-2(Mt) / IF-2Mt / IF2(mt)


Mass: 59602.836 Da / Num. of mol.: 1 / Source: (natural) Bos taurus / mammal / ウシ / / References: UniProt: P46198

Cellular component

Molecular function

Biological process

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentReconstruction method: SINGLE PARTICLE / Specimen type: VITREOUS ICE (CRYO EM)

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Sample preparation

Assembly of specimenName: Mammalian mitochondrial translation initiation factor 2 interaction with tRNA
Aggregation state: PARTICLE

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Electron microscopy imaging

MicroscopyMicroscope model: Tecnai F20
Electron gunElectron source: FEG / Accelerating voltage: 200 kV
Electron lensNominal magnification: 50000 X / Calibrated magnification: 50760 X
CameraType: Kodak S0163
RadiationDiffraction protocol: SINGLE WAVELENGTH / Monochromatic or laue m l: M / Scattering type: x-ray
Radiation wavelengthRelative weight: 1

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Processing

EM single particle entitySymmetry type: ASYMMETRIC
3D reconstructionMethod: Back Projection / Software: Spider / Resolution: 10.8 A / Number of particles: 121742 / Actual pixel size: 2.76 A/pix / CTF correction method: Every Micrograph
Number of atoms included #LASTProtein: 4180 / Nucleic acid: 1622 / Ligand: 0 / Solvent: 0 / Total: 5802

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