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    - PDB-3izd: Model of the large subunit RNA expansion segment ES27L-out based ... -

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    Basic information

    Entry
    Database: PDB / ID: 3izd
    TitleModel of the large subunit RNA expansion segment ES27L-out based on a 6.1 A cryo-EM map of Saccharomyces cerevisiae translating 80S ribosome. 3IZD is a small part (an expansion segment) which is in an alternative conformation to what is in already 3IZF.
    Descriptor151-MER
    KeywordsRIBOSOME / eukaryotic ribosome / homology modelling / de novo modeling / ribosomal RNA / rRNA / RNA expansion segments
    Specimen sourceSaccharomyces cerevisiae / yeast /
    MethodElectron microscopy (8.6 A resolution)
    AuthorsArmache, J.-P. / Jarasch, A. / Anger, A.M. / Villa, E. / Becker, T. / Bhushan, S. / Jossinet, F. / Habeck, M. / Dindar, G. / Franckenberg, S. / Marquez, V. / Mielke, T. / Thomm, M. / Berninghausen, O. / Beatrix, B. / Soeding, J. / Westhof, E. / Wilson, D.N. / Beckmann, R.
    CitationProc. Natl. Acad. Sci. U.S.A., 2010, 107, 19748-19753

    primary. Proc. Natl. Acad. Sci. U.S.A., 2010, 107, 19748-19753 StrPapers
    Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution.
    Jean-Paul Armache / Alexander Jarasch / Andreas M Anger / Elizabeth Villa / Thomas Becker / Shashi Bhushan / Fabrice Jossinet / Michael Habeck / Gülcin Dindar / Sibylle Franckenberg / Viter Marquez / Thorsten Mielke / Michael Thomm / Otto Berninghausen / Birgitta Beatrix / Johannes Söding / Eric Westhof / Daniel N Wilson / Roland Beckmann

    #1. Proc.Natl.Acad.Sci.USA, 2010, 107, 19754-19759 StrPapers
    Localization of eukaryote-specific ribosomal proteins in a 5.5-A cryo-EM map of the 80S eukaryotic ribosome.
    Armache, J.P. / Jarasch, A. / Anger, A.M. / Villa, E. / Becker, T. / Bhushan, S. / Jossinet, F. / Habeck, M. / Dindar, G. / Franckenberg, S. / Marquez, V. / Mielke, T. / Thomm, M. / Berninghausen, O. / Beatrix, B. / Soding, J. / Westhof, E. / Wilson, D.N. / Beckmann, R.

    DateDeposition: Oct 13, 2010 / Release: Dec 1, 2010 / Last modification: May 30, 2012

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    Assembly

    Deposited unit
    A: rRNA expansion segment ES27L in an "out" conformation

    48.7 kDa, 1 molecules
    Theoretical massNumber of molelcules
    Total
    (without water)
    48,7221
    Polyers48,7221
    Non-polymers00
    Water0

    Omokage search
    #1idetical with deposited unit / defined by author / Symmetry operations: (identity)x1
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    Components

    #1RNA chain / rRNA expansion segment ES27L in an "out" conformation / Source: Saccharomyces cerevisiae (gene. exp.) / References: GenBank: U53879.1

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    Experimental details

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    Experiment

    ExperimentMethod: ELECTRON MICROSCOPY

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    Sample preparation

    Sample preparationpH: 7.5

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    Electron microscopy imaging

    RadiationDiffraction protocol: SINGLE WAVELENGTH / Monochromatic or laue m l: M / Scattering type: x-ray
    Radiation wavelengthRelative weight: 1

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    Processing

    3D reconstructionResolution: 8.6 A
    Number of atoms included #LASTProtein: 0 / Nucleic acid: 3044 / Ligand: 0 / Solvent: 0 / Total: 3044

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