+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-30536 | ||||||||||||
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Title | Photosystem I-LHCI-LHCII of Chlamydomonas reinhardtii | ||||||||||||
Map data | |||||||||||||
Sample |
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Function / homology | Function and homology information plastoglobule / photosynthesis, light harvesting / chloroplast thylakoid lumen / photosynthesis, light harvesting in photosystem I / photosystem I reaction center / : / photosystem I / chloroplast envelope / photosystem I / photosystem II ...plastoglobule / photosynthesis, light harvesting / chloroplast thylakoid lumen / photosynthesis, light harvesting in photosystem I / photosystem I reaction center / : / photosystem I / chloroplast envelope / photosystem I / photosystem II / photosynthetic electron transport in photosystem I / chlorophyll binding / chloroplast thylakoid membrane / response to light stimulus / photosynthesis / 4 iron, 4 sulfur cluster binding / electron transfer activity / oxidoreductase activity / magnesium ion binding / metal ion binding Similarity search - Function | ||||||||||||
Biological species | Chlamydomonas reinhardtii (plant) / Chlamydomonas smithii (plant) | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.42 Å | ||||||||||||
Authors | Wang WD / Shen LL / Huang ZH / Han GY / Zhang X / Shen JR | ||||||||||||
Funding support | China, 3 items
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Citation | Journal: Science / Year: 2018 Title: Structure of the maize photosystem I supercomplex with light-harvesting complexes I and II. Authors: Xiaowei Pan / Jun Ma / Xiaodong Su / Peng Cao / Wenrui Chang / Zhenfeng Liu / Xinzheng Zhang / Mei Li / Abstract: Plants regulate photosynthetic light harvesting to maintain balanced energy flux into photosystems I and II (PSI and PSII). Under light conditions favoring PSII excitation, the PSII antenna, light- ...Plants regulate photosynthetic light harvesting to maintain balanced energy flux into photosystems I and II (PSI and PSII). Under light conditions favoring PSII excitation, the PSII antenna, light-harvesting complex II (LHCII), is phosphorylated and forms a supercomplex with PSI core and the PSI antenna, light-harvesting complex I (LHCI). Both LHCI and LHCII then transfer excitation energy to the PSI core. We report the structure of maize PSI-LHCI-LHCII solved by cryo-electron microscopy, revealing the recognition site between LHCII and PSI. The PSI subunits PsaN and PsaO are observed at the PSI-LHCI interface and the PSI-LHCII interface, respectively. Each subunit relays excitation to PSI core through a pair of chlorophyll molecules, thus revealing previously unseen paths for energy transfer between the antennas and the PSI core. | ||||||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_30536.map.gz | 168.2 MB | EMDB map data format | |
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Header (meta data) | emd-30536-v30.xml emd-30536.xml | 42.2 KB 42.2 KB | Display Display | EMDB header |
Images | emd_30536.png | 75.4 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-30536 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-30536 | HTTPS FTP |
-Related structure data
Related structure data | 7d0jMC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_30536.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Voxel size | X=Y=Z: 1.307 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
+Entire : Photosystem I-LHCI-LHCII supercomplex of Chlamydomonas reinhardtii
+Supramolecule #1: Photosystem I-LHCI-LHCII supercomplex of Chlamydomonas reinhardtii
+Supramolecule #2: Photosystem I
+Supramolecule #3: LHCI
+Supramolecule #4: LHCII
+Macromolecule #1: Photosystem I P700 chlorophyll a apoprotein A1
+Macromolecule #2: Photosystem I P700 chlorophyll a apoprotein A2
+Macromolecule #3: Photosystem I iron-sulfur center
+Macromolecule #4: Photosystem I reaction center subunit II, chloroplastic
+Macromolecule #5: Photosystem I reaction center subunit IV, chloroplastic
+Macromolecule #6: Photosystem I reaction center subunit F, Photosystem I reaction c...
+Macromolecule #7: Photosystem I reaction center subunit V, chloroplastic
+Macromolecule #8: Photosystem I reaction center subunit VI, chloroplastic
+Macromolecule #9: Photosystem I reaction center subunit VIII
+Macromolecule #10: Photosystem I reaction center subunit IX
+Macromolecule #11: Photosystem I reaction center subunit psaK, chloroplastic
+Macromolecule #12: PSI subunit V
+Macromolecule #13: Photosystem I subunit O
+Macromolecule #14: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #15: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #16: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #17: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #18: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #19: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #20: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #21: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #22: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #23: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #24: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #25: CHLOROPHYLL A
+Macromolecule #26: PHYLLOQUINONE
+Macromolecule #27: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
+Macromolecule #28: BETA-CAROTENE
+Macromolecule #29: IRON/SULFUR CLUSTER
+Macromolecule #30: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
+Macromolecule #31: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
+Macromolecule #32: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
+Macromolecule #33: CHLOROPHYLL B
+Macromolecule #34: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
+Macromolecule #35: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BE...
+Macromolecule #36: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY...
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELDBright-field microscopy |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 50.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
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Final angle assignment | Type: MAXIMUM LIKELIHOOD |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.42 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 283763 |